用于鉴定铜绿假单胞菌的分子靶标及其定量检测方法
By using novel molecular targets and their primer sets for PCR and qPCR methods, the problems of long testing cycles and inaccurate results in the detection of Pseudomonas aeruginosa have been solved, enabling rapid and accurate identification and quantification of Pseudomonas aeruginosa, thus improving detection efficiency and accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
- Filing Date
- 2021-12-20
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for detecting Pseudomonas aeruginosa suffer from problems such as long testing cycles, inaccurate results, and poor specificity, making it difficult to quickly and accurately identify and quantify Pseudomonas aeruginosa.
Novel and highly specific molecular targets and their corresponding PCR and qPCR methods were used to screen molecular targets of SEQ ID NO.1 to SEQ ID NO.4 through pan-genome analysis, and corresponding primer sets were designed for the identification and quantitative detection of Pseudomonas aeruginosa.
It enables rapid and accurate detection of Pseudomonas aeruginosa with a coverage rate of up to 100%. The detection time is short, the cost is low, the results are easy to interpret, the specificity is high, the stability is good, and the detection time is shortened from 3 to 5 days in traditional methods to about 15 hours.
Smart Images
Figure CN117327817B_ABST