Use of a nanofiber electrochemical sensor in detecting glucose

Abstract

The application discloses an application of a nanofiber electrochemical sensor in glucose detection, and a preparation method of the nanofiber electrochemical sensor, which comprises the following steps: (1) preparing composite cerium nanoparticles by using cerous nitrate as raw material; (2) preparing a base solution; (3) obtaining a uniform solution; (4) obtaining calcined nanometer material; (5) obtaining electrospun filaments; (6) obtaining nanometer cerium-molybdenum composite carbon fibers; (7) obtaining a modification solution; and (8) adding the modification solution dropwise to the surface of a glassy carbon electrode and drying, so that the application of the nanofiber electrochemical sensor in glucose detection is provided. The prepared nanofiber electrochemical sensor is used for glucose detection, has a shorter response time, a lower detection limit and a wider linear range, can realize simpler, faster and high-sensitivity glucose detection, has more stable detection performance, and can still maintain high sensitivity after multiple cycle detections.

Description

Technical Field

[0001] This invention belongs to the field of glucose detection technology, specifically the application of a nanofiber electrochemical sensor in glucose detection. Background Technology

[0002] Diabetes mellitus is a chronic metabolic disease characterized primarily by hyperglycemia. In recent years, the number of people with diabetes worldwide has been steadily increasing, reaching 463 million by 2019, with an average annual growth rate of 51%. Therefore, early prevention and care of diabetes should be given high priority.

[0003] The components of human blood have always been important information for evaluating physical health, and blood glucose levels are a key indicator for measuring metabolic capacity and clinical diagnosis of diabetes. Therefore, in order to manage and control the condition of diabetic patients, it is particularly important to adjust daily diet and exercise through real-time and continuous monitoring of the condition.

[0004] The effective and rapid detection of glucose plays an important role in biology, food, chemistry, clinical diagnosis and environmental protection. There have been many reports on enzyme-free electrochemical detection of glucose because enzyme-free electrochemical sensors have the characteristics of good stability, repeatability and simplicity.

[0005] Currently, commonly used glucose sensors can be divided into enzyme sensors and non-enzyme sensors. Although enzyme sensors have the characteristics of high efficiency and specificity, the conditions for enzyme survival are harsh. They can only react under mild conditions suitable for enzyme survival. Moreover, they are expensive, have poor stability, and are troublesome to immobilize.

[0006] In response, enzyme-free sensors have embarked on a path of continuous development. However, existing enzyme-free sensors have relatively slow current response speeds and low response current intensities, resulting in significantly poor stability in glucose detection.

[0007] Therefore, further improvements to existing technologies are needed. Summary of the Invention

[0008] The purpose of this invention is to provide an application of a nanofiber electrochemical sensor in glucose detection, thereby overcoming the shortcomings of the prior art.

[0009] The technical solution adopted in this invention is as follows: An application of a nanofiber electrochemical sensor in glucose detection, wherein the preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; (2) (NH4)6Mo7O 24·Add 4H2O to deionized water, stir to dissolve, and prepare a matrix solution; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 10-15 min to obtain a homogeneous solution; (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 2-3 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. (5) Add the calcined nanomaterials prepared in step (4) to a polyvinyl alcohol solution, and after ultrasonic dispersion treatment for 15-20 min, a dispersion is obtained, and then electrospinning is performed to obtain electrospun fibers; (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:25-28. The mass fraction of the Nafion solution is 5%; The volume ratio of the Nafion solution to anhydrous ethanol is 3:1-1.2; (8) Add the modification solution to the surface of the glassy carbon electrode and dry it.

[0010] As a further technical solution: the method for preparing composite cerium nanoparticles in step (1) is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture and stirred for 15-20 minutes. Then, the mixture was centrifuged at high speed for 10 minutes, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles.

[0011] As a further technical solution: the concentration of cerium nitrate is 0.3-0.4 mol / L; The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:30-35.

[0012] As a further technical solution: the matrix solution in step (2) contains (NH4)6Mo7O 24 The concentration is 1.2-1.6 mol / L.

[0013] As a further technical solution: the mass ratio of the composite cerium nanoparticles and the matrix solution in step (3) is 1:35-40.

[0014] As a further technical solution: the calcination temperature in the resistance furnace in step (4) is 420-440℃.

[0015] As a further technical solution: the mass ratio of the calcined nanomaterial and the polyvinyl alcohol solution in step (5) is 1:32-36; The polyvinyl alcohol solution has a mass fraction of 35-38%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h.

[0016] As a further technical solution: the high-temperature carbonization in step (6) is as follows: Under an inert atmosphere, the temperature is 750-780℃, and the temperature is maintained for 2 hours.

[0017] As a further technical solution: the inert atmosphere is nitrogen.

[0018] As a further technical solution: the amount of the modification liquid added to the surface of the glassy carbon electrode in step (8) is 0.2-0.3 g / cm²; The glassy carbon electrode has a diameter of 3 mm.

[0019] When using glassy carbon electrodes, the surface is polished by applying 1.0, 0.35, and 0.05 μm α-Al2O3 powders sequentially. Then, the electrode is ultrasonically cleaned in deionized water and acetone solutions for 10 minutes each. After cleaning, the electrode is removed and dried with nitrogen gas.

[0020] The nano-cerium-molybdenum composite carbon fiber prepared in this invention has a larger specific surface area, which can provide more active sites, thereby improving its electrochemical performance after modification on the glassy carbon electrode surface. This enables better electrocatalytic oxidation of glucose, thus broadening the application of the prepared sensor.

[0021] The nano-cerium-molybdenum composite carbon fiber prepared by this invention contains multiple components, including cerium, molybdenum and carbon. The interaction of these three components can effectively promote electron transfer efficiency and improve the electrocatalytic performance of glucose.

[0022] This invention introduces nano-cerium-molybdenum composite carbon fibers, which have a three-dimensional interwoven structure on the surface of the glassy carbon electrode, significantly increasing the contact area with the electrolyte. The structure is more complex, and the nano-cerium-molybdenum composite carbon fibers contain a large number of nanoparticles from the outside to the inside, as well as a large number of nanoscale pores, further increasing the number of exposed active sites and thus improving the electrochemical performance of the sensor. Beneficial effects

[0023] This invention provides an application of a nanofiber electrochemical sensor in glucose detection. The prepared nanofiber electrochemical sensor for glucose detection has a shorter response time, a lower detection limit, and a wider linear range, enabling simpler, faster, and more sensitive glucose detection. It also has more stable detection performance, maintaining high sensitivity even after multiple detection cycles, making it suitable for large-scale application. Attached Figure Description

[0024] Figure 1 This is a graph comparing the effect of different operating voltages on the response current intensity. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example

[0026] An application of a nanofiber electrochemical sensor in glucose detection, wherein the preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; the preparation method of composite cerium nanoparticles is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture, and the mixture was stirred for 15 min. Then, it was centrifuged at high speed for 10 min, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles. The concentration of cerium nitrate was 0.3 mol / L. The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:30. (2) (NH4)6Mo7O24· 4H₂O was added to deionized water, stirred and dissolved to prepare a matrix solution; the matrix solution contained (NH₄)₆Mo₇O. 24 The concentration is 1.2 mol / L; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 10 min to obtain a homogeneous solution; the mass ratio of composite cerium nanoparticles to matrix solution is 1:35. (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 2 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. The calcination temperature in the resistance furnace is 420℃. (5) Add the calcined nanomaterials prepared in step (4) to the polyvinyl alcohol solution, and after ultrasonic dispersion treatment for 15 min, obtain a dispersion, and then perform electrospinning to obtain electrospun fibers; the mass ratio of calcined nanomaterials to polyvinyl alcohol solution is 1:32. The polyvinyl alcohol solution has a mass fraction of 35%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h. (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; the high-temperature carbonization is carried out at 750°C for 2 hours under an inert atmosphere. The inert atmosphere is nitrogen. (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:25. The mass fraction of the Nafion solution is 5%; The Nafion solution was mixed with anhydrous ethanol in a volume ratio of 3:1. (8) Add the modification solution to the surface of the glassy carbon electrode and dry it; the amount of modification solution added to the surface of the glassy carbon electrode is 0.2 g / cm². The glassy carbon electrode has a diameter of 3 mm. Example

[0027] An application of a nanofiber electrochemical sensor in glucose detection, wherein the preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; the preparation method of composite cerium nanoparticles is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture, and the mixture was stirred for 16 min. Then, it was centrifuged at high speed for 10 min, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles. The concentration of cerium nitrate was 0.35 mol / L. The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:32. (2) (NH4)6Mo7O 24· 4H₂O was added to deionized water, stirred and dissolved to prepare a matrix solution; the matrix solution contained (NH₄)₆Mo₇O. 24 The concentration is 1.3 mol / L; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 12 min to obtain a homogeneous solution; the mass ratio of composite cerium nanoparticles to matrix solution is 1:36. (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 2.5 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. The calcination temperature in the resistance furnace is 430℃. (5) The calcined nanomaterials prepared in step (4) are added to the polyvinyl alcohol solution and ultrasonically dispersed for 18 min to obtain a dispersion. Then, electrospinning is performed to obtain electrospun fibers. The mass ratio of calcined nanomaterials to polyvinyl alcohol solution is 1:33. The polyvinyl alcohol solution had a mass fraction of 36%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h. (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; the high-temperature carbonization is carried out at a temperature of 760°C for 2 hours under an inert atmosphere. The inert atmosphere is nitrogen. (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:26. The mass fraction of the Nafion solution is 5%; The Nafion solution and anhydrous ethanol were mixed in a volume ratio of 3:1.1. (8) Add the modification solution to the surface of the glassy carbon electrode and dry it; the amount of modification solution added to the surface of the glassy carbon electrode is 0.24 g / cm². The glassy carbon electrode has a diameter of 3 mm. Example

[0028] An application of a nanofiber electrochemical sensor in glucose detection, wherein the preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; the preparation method of composite cerium nanoparticles is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture, and the mixture was stirred for 18 min. Then, it was centrifuged at high speed for 10 min, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles. The concentration of cerium nitrate was 0.36 mol / L. The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:33. (2) (NH4)6Mo7O 24· 4H₂O was added to deionized water, stirred and dissolved to prepare a matrix solution; the matrix solution contained (NH₄)₆Mo₇O. 24 The concentration is 1.5 mol / L; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 12 min to obtain a homogeneous solution; the mass ratio of composite cerium nanoparticles to matrix solution is 1:38. (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 2.5 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. The calcination temperature in the resistance furnace is 430℃. (5) The calcined nanomaterials prepared in step (4) are added to the polyvinyl alcohol solution and ultrasonically dispersed for 18 min to obtain a dispersion. Then, electrospinning is performed to obtain electrospun fibers. The mass ratio of calcined nanomaterials to polyvinyl alcohol solution is 1:35. The polyvinyl alcohol solution had a mass fraction of 36%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h. (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; the high-temperature carbonization is carried out at a temperature of 760°C for 2 hours under an inert atmosphere. The inert atmosphere is nitrogen. (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:27. The mass fraction of the Nafion solution is 5%; The Nafion solution and anhydrous ethanol were mixed in a volume ratio of 3:1.1. (8) Add the modification solution to the surface of the glassy carbon electrode and dry it; the amount of modification solution added to the surface of the glassy carbon electrode is 0.25 g / cm². The glassy carbon electrode has a diameter of 3 mm. Example

[0029] An application of a nanofiber electrochemical sensor in glucose detection, wherein the preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; the preparation method of composite cerium nanoparticles is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture, and the mixture was stirred for 20 min. Then, it was centrifuged at high speed for 10 min, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles. The concentration of cerium nitrate was 0.4 mol / L. The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:35. (2) (NH4)6Mo7O 24· 4H₂O was added to deionized water, stirred and dissolved to prepare a matrix solution; the matrix solution contained (NH₄)₆Mo₇O. 24 The concentration is 1.6 mol / L; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 15 min to obtain a homogeneous solution; the mass ratio of composite cerium nanoparticles to matrix solution is 1:40. (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 3 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. The calcination temperature in the resistance furnace is 440℃. (5) Add the calcined nanomaterials prepared in step (4) to the polyvinyl alcohol solution, and after ultrasonic dispersion treatment for 20 min, obtain a dispersion, and then perform electrospinning to obtain electrospun fibers; the mass ratio of calcined nanomaterials to polyvinyl alcohol solution is 1:36. The polyvinyl alcohol solution had a mass fraction of 38%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h. (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; the high-temperature carbonization is carried out at 780°C for 2 hours under an inert atmosphere. The inert atmosphere is nitrogen. (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:28. The mass fraction of the Nafion solution is 5%; The Nafion solution and anhydrous ethanol were mixed in a volume ratio of 3:1.2. (8) Add the modification solution to the surface of the glassy carbon electrode and dry it; the amount of modification solution added to the surface of the glassy carbon electrode is 0.3 g / cm². The glassy carbon electrode has a diameter of 3 mm.

[0030] Comparative Example 1: Based on Example 1, step (3) is adjusted so that no composite cerium nanoparticles are added, while the rest is the same as the technical solution in Example 1.

[0031] Comparative Example 2: Based on Example 1, step (5) is adjusted so that the calcined nanomaterial is replaced with an equal amount of composite cerium nanoparticles, and the rest of the technology is the same as the technical solution of Example 1.

[0032] Electrochemical tests were performed using a three-electrode system. In this invention and the examples, the glassy carbon electrode was used as the working electrode, the platinum wire as the counter electrode, and the calomel electrode as the reference electrode. The working voltage was set to 0.5 V, and the scan rate was 0.1 V / s. Electrochemical tests were conducted on the examples and comparative examples in 0.1 mol / L NaOH electrolyte, as shown in Table 1. Table 1

[0033] As can be seen from Table 1, the nanofiber electrochemical sensor prepared in this invention has a high response current intensity when performing electrochemical performance detection.

[0034] Continue the experiment and compare the current response time of the example and the comparative example. Table 2

[0035] As can be seen from Table 2, the nanofiber electrochemical sensor prepared in this invention has a faster response speed.

[0036] The experiment continued, using Example 1 as the base sample, to compare the effect of different operating voltages on the response current intensity: Table 3

[0037] As can be seen from Table 3, different operating voltages have different effects on the response current intensity.

[0038] The experiment continued, using Example 1 as the base sample, to compare the effect of different operating voltages on the response current intensity, such as... Figure 1 .

[0039] The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the scope of implementation shown. Any changes made in accordance with the concept of the present invention, or equivalent embodiments modified to have equivalent changes, shall be within the protection scope of the present invention as long as they do not exceed the spirit covered by the specification.

Claims

1. An application of a nanofiber electrochemical sensor in glucose detection, characterized in that: The preparation method of the nanofiber electrochemical sensor includes the following steps: (1) Composite cerium nanoparticles were prepared using cerium nitrate as a raw material; (2) (NH4)6Mo7O 24· Add 4H2O to deionized water, stir to dissolve, and prepare a matrix solution; (3) Add the composite cerium nanoparticles prepared in step (1) to the matrix solution and sonicate for 10-15 min to obtain a homogeneous solution; (4) The homogeneous solution obtained in step (1) is dried by rotary evaporation, and then placed in a resistance furnace for calcination for 2-3 hours. After natural cooling, the material is discharged to obtain calcined nanomaterials. (5) Add the calcined nanomaterials prepared in step (4) to a polyvinyl alcohol solution, and after ultrasonic dispersion treatment for 15-20 min, a dispersion is obtained, and then electrospinning is performed to obtain electrospun fibers; (6) The electrospun fibers obtained in step (5) are subjected to high-temperature carbonization to obtain nano-cerium molybdenum composite carbon fibers; (7) Add the nano-cerium molybdenum composite carbon fiber obtained in step (6) to the Nafion solution, add anhydrous ethanol, and continue stirring until homogeneous to obtain the modified solution; The mass ratio of nano-cerium molybdenum composite carbon fiber to Nafion solution is 1:25-28. The Nafion solution has a mass fraction of 5%; The volume ratio of the Nafion solution to anhydrous ethanol is 3:1-1.2; (8) Add the modification solution to the surface of the glassy carbon electrode and dry it.

2. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The method for preparing composite cerium nanoparticles in step (1) is as follows: First, cerium nitrate is added to deionized water to obtain a cerium nitrate solution; 1,3,5-Pyromellitic acid was added to anhydrous ethanol solution to obtain an alcohol mixture solution; Cerium nitrate solution was added dropwise to an alcohol mixture and stirred for 15-20 minutes. Then, the mixture was centrifuged at high speed for 10 minutes, allowed to stand for 1 hour, filtered, washed, and dried to obtain composite cerium nanoparticles.

3. The application of the nanofiber electrochemical sensor according to claim 2 in glucose detection, characterized in that: The concentration of cerium nitrate is 0.3-0.4 mol / L; The 1,3,5-pyromellitic acid was mixed with an anhydrous ethanol solution of 45% by mass at a mass ratio of 1:

15. The volume ratio of the cerium nitrate solution to the alcohol mixture is 1:30-35.

4. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The matrix solution described in step (2) contains (NH4)6Mo7O 24 The concentration is 1.2-1.6 mol / L.

5. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: In step (3), the mass ratio of the composite cerium nanoparticles to the matrix solution is 1:35-40.

6. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The calcination temperature in the resistance furnace in step (4) is 420-440℃.

7. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The mass ratio of the calcined nanomaterials and polyvinyl alcohol solution in step (5) is 1:32-36; The polyvinyl alcohol solution has a mass fraction of 35-38%. The electrospinning process involves adding the dispersion to a 5 mL plastic syringe and performing electrospinning at 20 kV and a stainless steel needle insertion speed of 0.32 mL / h.

8. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The high-temperature carbonization in step (6) is as follows: Under an inert atmosphere, the temperature is 750-780℃, and the temperature is maintained for 2 hours.

9. The application of the nanofiber electrochemical sensor according to claim 8 in glucose detection, characterized in that: The inert atmosphere is nitrogen.

10. The application of the nanofiber electrochemical sensor according to claim 1 in glucose detection, characterized in that: The amount of the modification solution added to the surface of the glassy carbon electrode in step (8) is 0.2-0.3 g / cm². The glassy carbon electrode has a diameter of 3 mm.

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