Method for improving tobacco seed vigor based on nano-copper oxide initiation

Abstract

The application belongs to the technical field of seed treatment and relates to a method for improving tobacco seed activity based on nano copper oxide initiation, which comprises the following steps: S1, weighing nano copper particles, dispersing in water, and preparing a solution with a specific concentration; S2, fully stirring the solution, then putting it into an ultrasonic device for ultrasonic dispersion to prepare a nano copper oxide suspension; S3, taking tobacco seeds, immersing them in the nano copper oxide suspension, stirring, and initiating in the dark; S4, pouring the initiated tobacco seeds into a nylon mesh bag and washing them with clean water for 3-4 times; and S5, putting the washed tobacco seeds into an oven for air drying until no obvious moisture is present on the surface to obtain nano-initiated tobacco seeds. The method for improving tobacco seed activity based on nano copper oxide initiation proposed in the application disperses nano copper oxide particles with a specific concentration in an aqueous solution, prepares a nano suspension of the particles after ultrasonic oscillation, and then uses the solution for tobacco seed initiation to improve the germination rate and germination uniformity of tobacco seeds.

Description

Technical Field

[0001] This invention belongs to the field of seed treatment technology, and more specifically, relates to a method for improving tobacco seed vigor based on nano-copper oxide initiation. Background Technology

[0002] Tobacco is one of my country's important economic crops, and its healthy and stable development has a significant impact on the national rural economic development and farmers' income. Tobacco seeds are the source of the tobacco industry, and their quality is the foundation for the industry's healthy and stable growth. Seed vigor is an important indicator of seed quality and a key factor in increasing crop yield. High-vigor seeds have stronger resistance to adverse conditions, significant growth advantages, and production potential. Therefore, conducting research on technologies and theories to improve seed vigor has extremely important theoretical value and practical significance.

[0003] Seed priming treatment can effectively improve seed quality, accelerate seed germination, increase seed germination uniformity, and enhance seed adaptability to adverse environmental conditions. In recent years, with the development of nanotechnology and its application in agriculture, especially in seed treatment, it has become one of the most promising tools for promoting sustainable agricultural development. International multinational seed groups have begun research and patent planning on nano-priming technology. How to utilize nano-priming technology to improve tobacco seed vigor and its commercial applications is a hot topic in seed technology research and an important aspect of further improving tobacco seed quality, increasing germination rate, and promoting the development of tobacco seed technology.

[0004] In view of the above, this application proposes a method for improving tobacco seed vigor based on nano-copper oxide initiation. Summary of the Invention

[0005] The purpose of this invention is to propose a method for improving tobacco seed vigor based on nano-copper oxide initiation. The main objective is to propose a method that can induce tobacco seeds through nano-suspension, thereby improving the germination rate and uniformity of tobacco seeds. This aims to solve the technical problem of inconsistent germination rates during tobacco floating seedling cultivation, especially after encountering adverse conditions, resulting in uneven germination and increased costs for thinning and other seedling cultivation methods.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] A method for improving tobacco seed vigor based on nano-copper oxide initiation, the method comprising the following steps:

[0008] S1. Weigh out the nano-copper oxide particles, add water to disperse them, and prepare a solution of a specific concentration.

[0009] S2. Stir the above solution thoroughly, then place it in an ultrasonicator for ultrasonic dispersion to prepare copper oxide nano suspension;

[0010] S3. Take tobacco seeds, immerse them in copper oxide nano suspension, stir, and initiate in the dark;

[0011] S4. Pour the ignited tobacco seeds into a nylon mesh bag and rinse with clean water 3-4 times.

[0012] S5. Place the cleaned tobacco seeds in an oven and dry them with forced air until there is no obvious moisture on the surface to obtain nano-initiated tobacco seeds.

[0013] Furthermore, in step S1, the particle size of the nano-copper oxide particles is 50 nm, and the concentration of the prepared solution is 50–100 mg / L.

[0014] Furthermore, in step S2, the ultrasonic dispersion time after stirring the nanosolution is 30 min.

[0015] Furthermore, in step S3, the volume ratio of the nano-suspension to tobacco seeds should be greater than 2:1, and the light-shielding initiation time should be 22–24 h.

[0016] Furthermore, the air-drying time in step S5 is 4 to 6 hours.

[0017] Compared with the prior art, the present invention has at least the following beneficial effects:

[0018] This invention proposes a method for enhancing tobacco seed vigor based on nano-copper oxide initiation.

[0019] A method was developed to disperse copper oxide nanoparticles of a specific concentration in an aqueous solution, prepare a nano-suspension by ultrasonic vibration, and then use the solution to initiate germination in tobacco seeds, thereby improving the germination rate and uniformity of tobacco seeds. Attached Figure Description

[0020] Figure 1 is a schematic diagram of the process of the method for improving tobacco seed vigor based on nano-copper oxide initiation according to the present invention. Detailed Implementation

[0021] As shown in Figure 1, to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and are not intended to limit the present invention.

[0022] The test materials used in the following examples were MS Yunyan 87 tobacco seeds from different years produced by Yuxi China Tobacco Seed Co., Ltd.

[0023] Example 1

[0024] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0025] The method, specifically, involves weighing 50 nm copper oxide, dispersing it in water to prepare a 10 mg / L aqueous solution, stirring thoroughly, and then ultrasonically dispersing it in an ultrasonic oscillator for 30 min to prepare the oxide solution.

[0026] Copper nano-suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned nano-copper oxide suspension. Stir thoroughly and initiate in the dark for 24 h. Pour the seeds into a nylon mesh bag and rinse with water 3-4 times to remove the nanomaterials on the seed surface. Place the seeds in a forced-air drying oven at 30℃ for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed. Perform standard germination tests on the above tobacco seeds according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". Count the germination results on the 5th and 7th days. The standard for normal seedlings is when the cotyledons are green at 5 days and when the two cotyledons have unfolded and the radicle is normal at 7 days.

[0027] Example 2

[0028] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0029] The method is as follows: Weigh 50 nm copper oxide, disperse it in water to prepare a 20 mg / L aqueous solution, stir thoroughly, and then sonicate it in an ultrasonic oscillator for 30 min to prepare a copper oxide nano suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned copper oxide nano suspension. Stir thoroughly and initiate the reaction in the dark for 24 h. Pour the seeds into a nylon mesh bag, rinse them 3-4 times with water to remove the nanomaterials on the seed surface, and place them in a forced-air drying oven at 30℃ for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed.

[0030] The above-mentioned tobacco seeds were tested for standard germination according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". The germination results on the 5th and 7th days were statistically analyzed. On the 5th day, the cotyledons were green and the seedlings were considered to be normal. On the 7th day, the seedlings were considered to be normal when the cotyledons were fully expanded and the radicle was normal.

[0031] Example 3

[0032] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0033] The method is as follows: Weigh 50 nm copper oxide, disperse it in water to prepare a 50 mg / L aqueous solution, stir thoroughly, and then ultrasonically disperse it in an ultrasonic shaker for 30 min to prepare a copper oxide nano suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned copper oxide nano suspension. Stir thoroughly and initiate the process in the dark for 24 h. Pour the seeds into a nylon mesh bag, rinse them 3-4 times with clean water to remove the nanomaterials on the seed surface, and place them in a forced-air drying oven at 30℃ for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed. Perform standard germination tests on the above tobacco seeds according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". Count the germination results of the seeds on the 5th and 7th days. The standard for normal seedlings is the appearance of green cotyledons on the 5th day and the standard for normal seedlings is the unfolding of two cotyledons and normal radicle on the 7th day.

[0034] Example 4

[0035] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0036] The method is as follows: Weigh 50 nm copper oxide, disperse it in water to prepare a 100 mg / L aqueous solution, stir thoroughly, and then sonicate it in an ultrasonic oscillator for 30 min to prepare a copper oxide nano suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned copper oxide nano suspension. Stir thoroughly and initiate the reaction in the dark for 24 h. Pour the seeds into a nylon mesh bag, rinse them 3-4 times with water to remove the nanomaterials on the seed surface, and place them in a forced-air drying oven at 30°C for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed.

[0037] The above-mentioned tobacco seeds were subjected to standard germination tests according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". The germination results on the 5th and 7th days were statistically analyzed. Germination on the 5th day was based on the cotyledons.

[0038] The standard for normal seedlings is the appearance of green leaves. The standard for normal seedlings is the unfolding of two cotyledons and the normal development of the radicle at 7 days.

[0039] Example 5

[0040] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0041] The method is as follows: Weigh 50 nm copper oxide, disperse it in water to prepare a 200 mg / L aqueous solution, stir thoroughly, and then sonicate it in an ultrasonic oscillator for 30 min to prepare a copper oxide nano suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned copper oxide nano suspension. Stir thoroughly and initiate the reaction in the dark for 24 h. Pour the seeds into a nylon mesh bag, rinse them 3-4 times with water to remove the nanomaterials on the seed surface, and place them in a forced-air drying oven at 30°C for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed.

[0042] The above-mentioned tobacco seeds were tested for standard germination according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". The germination results on the 5th and 7th days were statistically analyzed. On the 5th day, the cotyledons were green and the seedlings were considered to be normal. On the 7th day, the seedlings were considered to be normal when the cotyledons were fully expanded and the radicle was normal.

[0043] Example 6

[0044] The present invention describes a method for improving tobacco seed vigor based on nano-copper oxide initiation.

[0045] The method is as follows: Weigh 50 nm copper oxide, disperse it in water to prepare a 500 mg / L aqueous solution, stir thoroughly, and then sonicate it in an ultrasonic oscillator for 30 min to prepare a copper oxide nano suspension. Weigh 1 g of MS Yunyan 87 seeds and add them to 100 ml of the above-mentioned copper oxide nano suspension. Stir thoroughly and initiate the reaction in the dark for 24 h. Pour the seeds into a nylon mesh bag, rinse them 3-4 times with water to remove the nanomaterials on the seed surface, and place them in a forced-air drying oven at 30°C for 6 h until there is no moisture on the seed surface and the seeds can be easily dispersed.

[0046] The above-mentioned tobacco seeds were tested for standard germination according to the conditions specified in the national standard GB / T21138-2019 "Tobacco Seeds". The germination results on the 5th and 7th days were statistically analyzed. On the 5th day, the cotyledons were green and the seedlings were considered to be normal. On the 7th day, the seedlings were considered to be normal when the cotyledons were fully expanded and the radicle was normal.

[0047] Control group 1

[0048] The difference between Control Group 1 and Examples 1-6 is that Control Group 1 used uninitiated seeds to directly conduct a standard germination test.

[0049] Control group 2

[0050] The difference between control group 2 and examples 1-6 is that control group 2 was initiated with water.

[0051] The statistical results are shown in Table 1:

[0052] As can be seen from Table 1, compared with the undeveloped seeds in control group 1, after 10...

[0053] After priming with a suspension of 50 nm copper oxide particles at concentrations ranging from mg / L to 500 mg / L, the germination potential of tobacco seeds significantly increased by 13.67%–26.67% after 5 days. This increased germination rate, indicating improved uniformity of seed germination, demonstrates the effective priming effect of the copper oxide particle suspension. Compared to the control group primed with water, priming with 10 mg / L–20 mg / L copper oxide nanoparticles improved germination ability comparable to water, while priming with 50 mg / L–200 mg / L copper oxide nanoparticles was superior to water priming, increasing germination potential by 4.34%–7.67% after 5 days. In conclusion, priming with 50 nm copper oxide particles can improve the germination rate and uniformity of tobacco seeds.

[0054] This means improving seed vigor, thereby enhancing seedling quality and saving costs for tobacco floating seedling cultivation.

[0055] Table 1. Effects of different concentrations of nano-copper oxide on tobacco seed germination.

[0056]

[0057] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for improving tobacco seed vigor based on nano-copper oxide initiation, characterized in that: The method includes The following steps: S1. Weigh out the nano-copper oxide particles, add water to disperse them, and prepare a solution of a specific concentration. S2. Stir the above solution thoroughly, then place it in an ultrasonicator for ultrasonic dispersion to prepare copper oxide nano-suspension. liquid; S3. Take tobacco seeds, immerse them in copper oxide nano suspension, stir, and initiate in the dark; S4. Pour the ignited tobacco seeds into a nylon mesh bag and rinse with clean water 3-4 times. S5. Place the cleaned tobacco seeds in an oven and dry them with forced air until there is no obvious moisture on the surface, thus obtaining nano-initiators. Later tobacco seeds; In step S1, the particle size of the nano-copper oxide particles is 50 nm, and the concentration of the prepared solution is... 50–100 mg / L.

2. The method for improving tobacco seed vigor based on nano-copper oxide initiation according to claim 1, wherein... The characteristic is that the ultrasonic dispersion time of the nanosolution after stirring in step S2 is 30 min.

3. The method for improving tobacco seed vigor based on nano-copper oxide initiation according to claim 1, wherein... The characteristic is that, in step S3, the volume ratio of the nano-suspension to tobacco seeds should be greater than 2:1, and light-shielding should be used. The onset time is 22-24 hours.

4. The method for improving tobacco seed vigor based on nano-copper oxide initiation according to claim 1, wherein... The characteristic is that the air-drying time in step S5 is 4 to 6 hours.

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