A synergistic sterilization method and apparatus

Abstract

To solve the technical problem of poor sterilization effect of the traditional sterilization method of the high-pressure corrosion experiment kettle, the embodiment of the present application provides a synergistic sterilization method and device, the method comprising: under the combined action of high pressure, ultraviolet light irradiation and a solution containing a trace amount of bactericide, killing microorganisms in the high-pressure corrosion experiment kettle; the synergistic sterilization device comprises: a high-pressure-resistant sealed transparent container for placing the solution containing a trace amount of bactericide in the high-pressure corrosion experiment kettle for generating high pressure, and an ultraviolet generating device is arranged in the high-pressure-resistant sealed transparent container. Thus, the embodiment of the present application realizes efficient sterilization of microorganisms in the high-pressure corrosion experiment kettle under the combined action of high pressure, ultraviolet light irradiation and a solution containing a trace amount of bactericide, significantly improves the sterilization efficiency at room temperature, and reduces the influence of microorganisms in the high-pressure kettle on the reaction and experiment in the high-pressure kettle.

Description

Technical Field

[0001] This invention relates to a synergistic sterilization method and apparatus. Background Technology

[0002] During oil and gas field development, pipeline metal materials face severe corrosion problems. The harsh corrosive environment of oil and gas fields exposes metal pipelines to threats from high temperatures, high pressures, CO2, H2S, and microorganisms, resulting in significant economic losses. The unique and severe environment of oil and gas fields presents enormous challenges to pipeline corrosion monitoring. Currently, the causes, influencing factors, and corrosion mechanisms of pipe materials remain not fully understood. The special environment of oil and gas fields makes it difficult to truly understand and derive the corrosion mechanisms of steel from data obtained in the field. Laboratory studies of steel corrosion behavior and mechanisms play a crucial role in revealing the corrosion mechanisms of steel in real-world environments and subsequent protection measures. Moreover, the controllable experimental conditions allow for a better understanding of the roles of various influencing factors in the metal corrosion process.

[0003] The unique high-pressure environment of oil and gas fields makes high-pressure equipment indispensable for laboratory research on the corrosion mechanisms and protection of steel. High-pressure corrosion reactors are essential for studying corrosion in oil and gas fields. Currently, using high-pressure corrosion reactors to study the corrosion behavior and mechanisms of metals and then applying this knowledge to real-world environments plays a crucial role in guiding metal corrosion and protection in oil and gas fields. However, it is often found that laboratory data deviates significantly from field data in oil and gas fields. This is primarily due to the introduction of additional microorganisms into the experimental system. Microorganisms are ubiquitous in the natural environment, and many bacteria, such as sulfate-reducing bacteria (SRB), saprophytic bacteria (TGB), and iron bacteria (IB), can cause metal corrosion. Therefore, the impact of microorganisms on metal corrosion must be considered in laboratory research. Especially when studying bacterial corrosion, eliminating additional bacteria introduced from the natural environment is essential. Currently, almost all laboratories sterilize experimental equipment and samples using autoclaves, disinfectants, or ultraviolet irradiation before conducting corrosion experiments. However, for high-pressure steam sterilizers, steam causes rapid oxidation and corrosion of the test pieces and specimens, affecting the accuracy of subsequent corrosion experiments. Furthermore, the corrosion test vessel contains power supplies, rubber components, and electronic control systems, which are easily damaged or malfunction under high-temperature, high-pressure steam conditions, making it unsuitable for high-pressure corrosion testing. For ultraviolet (UV) sterilization, the complex internal structures of the test pieces, specimens, and corrosion test vessel can lead to insufficient UV irradiation and poor sterilization, as well as poor sterilization of the solution within the vessel. As for bactericides, low concentrations are ineffective, while high concentrations can negatively impact the experiment, and uneven distribution also results in poor sterilization. Moreover, long-term use of UV light and bactericides can lead to bacterial resistance, further reducing sterilization effectiveness. Because these sterilization methods lack effective consideration of microbial influence and fail to effectively sterilize the apparatus and solutions, microorganisms introduced from outside the experimental system inevitably have a significant impact on the experimental results. Summary of the Invention

[0004] To address the technical problem of poor sterilization effect of traditional sterilization methods for high-pressure corrosion test vessels, this invention provides a synergistic sterilization method and apparatus.

[0005] The embodiments of the present invention are achieved through the following technical solutions:

[0006] In a first aspect, embodiments of the present invention provide a synergistic sterilization method, comprising:

[0007] Microorganisms in the high-pressure corrosion test vessel are killed by the combined action of high pressure, ultraviolet light irradiation, and a solution containing trace amounts of bactericide.

[0008] Furthermore, the high voltage is 1-50 MPa, preferably 5 MPa; the intensity of the ultraviolet light irradiation is 2-400 μW / cm². 2 30μW / cm is preferred 2 The duration of ultraviolet light irradiation is 0.5-2 hours, preferably 1 hour.

[0009] Furthermore, the microorganisms include one or more of sulfate-reducing bacteria, iron bacteria, and / or saprophytic bacteria.

[0010] Furthermore, the bactericide in the solution containing trace amounts of bactericide is an aldehyde and / or a quaternary ammonium salt.

[0011] Furthermore, the bactericide is glyoxal.

[0012] Furthermore, the medium for the disinfectant is water.

[0013] Furthermore, the concentration of glyoxal in the solution containing trace amounts of glyoxal is 1-10 ppm, preferably 1 ppm.

[0014] Secondly, embodiments of the present invention provide a synergistic sterilization apparatus for implementing the method, comprising:

[0015] A high-pressure resistant, sealed, transparent container is used to place a solution containing trace amounts of bactericide into a high-pressure corrosion test vessel used to generate high pressure for sterilization. The high-pressure resistant, sealed, transparent container is equipped with an ultraviolet generator.

[0016] Furthermore, the high-pressure resistant sealed transparent container is made of transparent glass that can withstand high pressure of 1-20 MPa.

[0017] Furthermore, the ultraviolet generating device includes an ultraviolet lamp; the power of the ultraviolet lamp is 10W, and the irradiation intensity of the ultraviolet lamp is 30μW / cm². 2 The pressure of the high-pressure corrosion test vessel is 5 MPa.

[0018] Compared with the prior art, the embodiments of the present invention have the following advantages and beneficial effects:

[0019] The present invention discloses a synergistic sterilization method and apparatus that achieves highly efficient sterilization of microorganisms in a high-pressure corrosion test vessel through the combined action of high pressure, ultraviolet light irradiation, and a solution containing trace amounts of bactericide. It can significantly improve sterilization efficiency at room temperature and reduce the impact of microorganisms in the high-pressure vessel on the reaction and experiment inside the high-pressure vessel. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the exemplary embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the synergistic sterilization device inside the high-pressure corrosion test vessel.

[0022] The attached diagram shows the markings and corresponding component names:

[0023] 1-Wire, 2-Solution containing trace amounts of bactericide, 3-High-pressure corrosion test vessel, 4-High-pressure resistant sealed transparent container, 5-Ultraviolet generator. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of the present invention are only used to explain the present invention and are not intended to limit the present invention.

[0025] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the invention. However, it will be apparent to those skilled in the art that these specific details are not necessary to practice the invention. In other embodiments, well-known structures, circuits, materials, or methods have not been specifically described in order to avoid obscuring the invention.

[0026] Throughout this specification, references to "an embodiment," "an example," or "an example" mean that a particular feature, structure, or characteristic described in connection with that embodiment or example is included in at least one embodiment of the invention. Therefore, the phrases "an embodiment," "an example," "an example," or "an example" appearing in various places throughout the specification do not necessarily refer to the same embodiment or example. Furthermore, specific features, structures, or characteristics can be combined in one or more embodiments or examples in any suitable combination and / or sub-combination. Moreover, those skilled in the art will understand that the illustrations provided herein are for illustrative purposes and are not necessarily drawn to scale. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0027] In the description of this invention, the terms "front", "rear", "left", "right", "up", "down", "vertical", "horizontal", "high", "low", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0028] Example

[0029] To address the technical problem of poor sterilization effect of traditional sterilization methods for high-pressure corrosion test vessels, in a first aspect, embodiments of the present invention provide a synergistic sterilization method, comprising:

[0030] Microorganisms in the high-pressure corrosion test vessel are killed by the combined action of high pressure, ultraviolet light irradiation, and a solution containing trace amounts of bactericide.

[0031] Therefore, the embodiments of the present invention achieve highly efficient sterilization of microorganisms in high-pressure corrosion test vessels through the combined action of high pressure, ultraviolet light irradiation, and a solution containing trace amounts of bactericide. The sterilization efficiency can be significantly improved at room temperature, reducing the impact of microorganisms in the high-pressure vessel on the reaction and experiment inside the high-pressure vessel.

[0032] Furthermore, the high voltage is 1-50 MPa, preferably 5 MPa; the intensity of the ultraviolet light irradiation is 2-400 μW / cm². 2 30μW / cm is preferred 2 The duration of ultraviolet light irradiation is 0.5-2 hours, preferably 1 hour.

[0033] Furthermore, the microorganisms include one or more of sulfate-reducing bacteria, iron bacteria, and / or saprophytic bacteria.

[0034] Furthermore, the bactericide in the solution containing trace amounts of bactericide is an aldehyde and / or a quaternary ammonium salt.

[0035] Furthermore, the bactericide is glyoxal.

[0036] Furthermore, the medium for the disinfectant is water.

[0037] Furthermore, the concentration of glyoxal in the solution containing trace amounts of glyoxal is 1-10 ppm, preferably 1 ppm.

[0038] Secondly, based on the above method, embodiments of the present invention provide a synergistic sterilization device for the method described above, referencing... Figure 1As shown, it includes: a high-pressure resistant, sealed, transparent container for placing into a solution containing trace amounts of bactericide in a high-pressure corrosion test vessel used to generate high pressure, and the high-pressure resistant, sealed, transparent container is equipped with an ultraviolet generator.

[0039] refer to Figure 1 As shown, the synergistic sterilization device includes an ultraviolet generator 5 and a high-pressure resistant sealed transparent container 4. The ultraviolet generator is located inside the high-pressure resistant sealed transparent container and is connected to an external power source through a wire 1. The high-pressure corrosion test vessel 3 is used to hold a solution 2 containing trace amounts of bactericide.

[0040] Optionally, the high-pressure resistant sealed transparent container is made of transparent glass that can withstand high pressure of 1-20 MPa.

[0041] Optionally, the ultraviolet generating device includes an ultraviolet lamp; the ultraviolet lamp has a power of 10W and an irradiation intensity of 30μW / cm². 2 The pressure of the high-pressure corrosion test vessel is 5 MPa.

[0042] Example 1

[0043] A synergistic sterilization method, comprising:

[0044] At a high pressure of 1 MPa and a pressure of 2 μW / cm 2 Microorganisms in the high-pressure corrosion test vessel were killed by the combined action of 0.5 hours of ultraviolet light irradiation and an aqueous solution containing 10 ppm of quaternary ammonium salt. The microorganisms were iron bacteria.

[0045] Example 2

[0046] A synergistic sterilization method, comprising:

[0047] Under a high pressure of 30 MPa and a pressure of 400 μW / cm 2 Microorganisms in the high-pressure corrosion test vessel were killed by the combined action of 0.5 hours of ultraviolet light irradiation and an aqueous solution containing 5 ppm aldehyde. The microorganisms were saprophytic bacteria.

[0048] Example 3

[0049] A synergistic sterilization method, comprising:

[0050] Under a high pressure of 5 MPa and a pressure of 30 μW / cm 2 Microorganisms in the high-pressure corrosion test vessel were killed by the combined action of 1 hour of ultraviolet light irradiation and an aqueous solution containing 1 ppm glyoxal. The microorganisms were sulfate-reducing bacteria.

[0051] To verify the effectiveness of high pressure, ultraviolet light irradiation, and solutions containing trace amounts of bactericides in killing microorganisms, the following experimental example is given.

[0052] Example 1: Construction of a Co-sterilization Device

[0053] Building such Figure 1 The synergistic sterilization device shown places an ultraviolet lamp inside a sealed, high-pressure resistant transparent glass container, which is then placed on one side of an autoclave. A solution containing a trace amount of bactericide is placed inside the autoclave, and a test piece containing bacteria, made of the same material as the autoclave wall, is placed inside to simulate the amount of viable bacteria on the autoclave wall. The high-pressure corrosion test utilizes the pressure inside the autoclave, ultraviolet light, and the bactericide to synergistically achieve sterilization at room temperature.

[0054] Distilled water was used for the test, and API RP-38 medium was used for the sulfate-reducing bacteria (SRB) culture medium. The bacterial content was tested using the stepwise dilution method.

[0055] Experiment Example 2: Sterilization effect test under ultraviolet (UV) irradiation only

[0056] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 After incubation under 10W UV light for 1 hour, the bacterial count was tested.

[0057] Experiment Example 3: Sterilization effect test under high pressure corrosion test vessel conditions only

[0058] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 The bacterial count was controlled at 5 MPa in the autoclave, and the bacterial content was tested after 1 hour of incubation.

[0059] Experiment 4 only tested the bactericidal effect of the bactericide.

[0060] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 Bacterial count / mL, glyoxal 4 mg, incubated for 1 hour and then tested for bacterial content.

[0061] Experiment Example 5: Test of the sterilization effect of ultraviolet light irradiation + high-pressure corrosion test vessel

[0062] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 The bacterial count was measured at 5 MPa in the autoclave, and the bacteria were cultured under 10W UV light for 1 hour before testing the bacterial content.

[0063] Experiment Example 6: Synergistic Bactericidal Effect Test of UV + Bactericide

[0064] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 After incubating at 4 mg glyoxal per mL under 10W UV irradiation for 1 hour, the bacterial count was tested.

[0065] Experiment Example 7: Test of the Synergistic Sterilization Effect of Pressure and Bactericide in a High-Pressure Corrosion Test Vessel

[0066] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 Bacterial count / mL, glyoxal 4mg, pressure controlled at 5MPa in autoclave, bacterial content tested after 1h.

[0067] Experiment Example 8: Test of the Synergistic Sterilization Effect of Pressure + Ultraviolet (UV) + Bactericide in a High-Pressure Corrosion Test Vessel

[0068] Based on Experiment 1, 5×10⁻⁶ particles were placed inside the high-pressure corrosion test vessel. 4 pcs / cm 2 A bacterial test strip and 4 liters of water were mixed with sulfate-reducing bacteria, resulting in an initial bacterial concentration of 25 × 10⁻⁶. 5 Bacterial count / mL, glyoxal 4 mg, 5 MPa pressure, 10 W UV light, bacterial content was tested after 1 hour. Table 1 shows the test results.

[0069] Table 1

[0070] Experimental Example 1 <![CDATA[25.0×10 5 ]]> <![CDATA[5.0×10 4 ]]> Experimental Example 2 <![CDATA[4.5×10 5 ]]> <![CDATA[4.5×10 3 ]]> Experimental Example 3 <![CDATA[9.5×10 5 ]]> <![CDATA[4.5×10 4 ]]> Experiment Example 4 <![CDATA[2.5×10 5 ]]> <![CDATA[2,5×10 4 ]]> Experimental Example 5 <![CDATA[4.5×10 5 ]]> <![CDATA[4.0×10 3 ]]> Experimental Example 6 <![CDATA[2.5×10 4 ]]> <![CDATA[2,5×10 3 ]]> Experimental Example 7 <![CDATA[2.5×10 5 ]]> <![CDATA[2,5×10 4 ]]> Experimental Example 8 <![CDATA[2.5×10 2 ]]> <![CDATA[2.5×10 1 ]]>

[0071] It can be seen that after 1 hour of UV irradiation alone, the number of viable bacteria in both the immobilized and solution decreased by one order of magnitude; after 1 hour of high-pressure sterilization alone, the number of viable bacteria in the immobilized state did not decrease by an order of magnitude, while the number of viable bacteria in the solution decreased by one order of magnitude; after 1 hour of sterilization with only a trace amount of sterilizing agent, the number of viable bacteria in the immobilized state did not decrease by an order of magnitude, while the number of viable bacteria in the solution decreased by one order of magnitude; after 1 hour of pressure + UV, the number of viable bacteria in both the immobilized and solution decreased by one order of magnitude; after 1 hour of pressure + sterilizing agent, the number of viable bacteria in the immobilized state did not decrease by an order of magnitude, while the number of viable bacteria in the solution decreased by one order of magnitude; after 1 hour of UV + sterilizing agent, the number of viable bacteria in the immobilized state decreased by one order of magnitude, while the number of viable bacteria in the solution decreased by two orders of magnitude; after 1 hour of the combined effects of 10W UV, 5MPa pressure, and 1ppm glyoxal, the number of viable bacteria in the immobilized state decreased by three orders of magnitude, while the number of viable bacteria in the solution decreased by four orders of magnitude. It can be seen that the best sterilization effect is achieved when high pressure, ultraviolet light irradiation, and trace amounts of bactericide work synergistically.

[0072] Therefore, the embodiments of the present invention achieve a highly efficient sterilization effect through the synergistic effect of ultraviolet light irradiation, the pressure inside the high-pressure corrosion test vessel, and the bactericide. The bacterial survival rate is reduced by 2-3 orders of magnitude compared with traditional methods. At the same time, by utilizing the synergistic effect of the three, the intensity of ultraviolet radiation is reduced, thereby reducing the impact of ultraviolet radiation intensity on the experiment inside the high-pressure vessel and improving the service life of the device. It has important application prospects in the field of microbial sterilization and control in high-pressure vessels simulating deep-sea environments and oil and gas field environments.

[0073] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A synergistic sterilization method, characterized in that, Used inside high-pressure corrosion test vessels, including: Microorganisms in the high-pressure corrosion test vessel are killed by the combined action of the working pressure generated by the high-pressure corrosion test vessel itself, ultraviolet light irradiation, and an aqueous solution containing trace amounts of bactericide. The working pressure is 5 MPa, the ultraviolet light irradiation intensity is 2-400 μW / cm², and the bactericide is glyoxal or quaternary ammonium salt, with a concentration of 1-10 ppm in the solution. The combined effect of pressure, ultraviolet light, and bactericide reduces the number of surviving microorganisms in the sterilized solution by 3-4 orders of magnitude.

2. The synergistic sterilization method as described in claim 1, characterized in that, The intensity of the ultraviolet light irradiation is preferably 30 μW / cm2; the irradiation time is 0.5-2h.

3. The synergistic sterilization method as described in claim 2, characterized in that, The ultraviolet light irradiation time was 1 hour.

4. The synergistic sterilization method as described in claim 2, characterized in that, The microorganisms include one or more of sulfate-reducing bacteria, iron bacteria, and / or saprophytic bacteria.

5. The synergistic sterilization method as described in claim 4, characterized in that, The medium for the disinfectant is water.

6. The synergistic sterilization method as described in claim 5, characterized in that, The concentration of glyoxal in the solution containing trace amounts of glyoxal is 1 ppm.

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