Plant tissue culture jar and control method thereof

Abstract

This invention discloses a plant tissue culture vessel and its control method. The plant tissue culture vessel has a shearing section inside its cavity for cutting adventitious roots, and a power unit outside the vessel. The power unit magnetically drives the shearing section to open and close the cut, separated by the vessel wall. In this invention, the use of a power unit to magnetically drive the shearing section eliminates the need for a traditional rotating shaft connection structure. This eliminates the need for a sealing structure between the plant tissue culture vessel and the power unit, improving the sealing of the vessel wall and completely preventing oil or microorganisms from the power unit from entering the vessel. This ensures that the nutrient solution inside the plant tissue culture vessel maintains a sterile environment for a long time, providing a stable and reliable environment for adventitious root growth, thereby significantly increasing the yield and quality of adventitious roots within the same culture cycle.

Description

Technical Field

[0001] This invention belongs to the technical field of plant tissue culture devices, specifically, it relates to a plant tissue culture vessel and its control method. Background Technology

[0002] With the continuous improvement of living standards, people are paying increasing attention to strengthening their constitution and maintaining health through the consumption of medicinal herbs with nourishing effects. Due to the huge population, the demand for medicinal plants has risen sharply. Furthermore, cultivating medicinal plants using traditional methods requires not only large amounts of land and a long growth cycle, but also a suitable climate. Any unsuitable conditions will limit and reduce the scale of medicinal plant cultivation and the yield of medicinal plants.

[0003] Therefore, researchers have developed a method and cultivation device for large-scale cultivation using isolated plant tissues or cells. By separating plant organs such as roots, stems, and leaves, and then placing them in a nutrient-rich culture medium while providing suitable temperature, light, and other environmental conditions, callus, adventitious buds, and adventitious roots are induced. Finally, these callus, adventitious buds, and adventitious roots are used as seeds for further cultivation in the device. This method overcomes the dependence on soil and climate conditions for medicinal plant cultivation and allows for large-scale cultivation using numerous devices, increasing the yield of medicinal plants.

[0004] In existing technologies, various cultivation tanks for cultivating adventitious roots have been designed and applied to the cultivation of adventitious roots of high-value medicinal materials such as ginseng and Panax notoginseng. To control the length of adventitious roots and prevent them from tangling, frequent trimming is necessary. Typically, a rotating shaft with a shearing blade at one end is inserted into the tank wall, and then connected to and driven from outside the tank.

[0005] A sealing ring is used between the rotating shaft and the tank wall to maintain the airtightness of the culture tank and prevent the liquid inside from being contaminated by the external environment. However, frequent rotation of the shaft can damage the elasticity of the sealing ring and wear it down, causing it to lose its sealing effect. This leads to the disruption of the sealed environment of the culture tank, contamination of the culture medium, and necrosis of adventitious roots.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] The purpose of this invention is to provide a plant tissue culture vessel to avoid the power unit from damaging the airtightness of the culture vessel.

[0008] The present invention also aims to provide a method for controlling plant tissue culture tanks, so as to accurately control the length of adventitious roots, ensure growth rate, and improve the yield and quality of adventitious roots.

[0009] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0010] A plant tissue culture vessel has a shearing part inside the vessel cavity for cutting adventitious roots, and a power unit outside the vessel. The power unit uses magnetic force to drive the shearing part to open and close the cut through the vessel wall.

[0011] Furthermore, the shearing section includes a fixed first blade and a second blade that can rotate relative to the first blade to form a cut.

[0012] The power unit is equipped with a magnetic suction device that can rotate around its central axis. The power unit and the second blade are arranged with their central axes coinciding. The magnetic suction device binds the second blade with a magnetic field through the can wall and rotates together.

[0013] Furthermore, the power unit also includes a stator and a rotor driven by the stator. The stator is fixed to the outer side of the tank wall, and the rotor is rotatably sleeved with the stator.

[0014] Furthermore, the rotor is configured in a ring shape and sleeved on the outer periphery of the stator, and the magnetic attractor is fixed in the rotor on the end face facing the tank wall.

[0015] Furthermore, the stator is configured as a ring shape and has through holes extending through both ends along its central axis, the rotor is configured as a long rod and sleeved in the through holes, and the magnetic attractor is fixed in the rotor at one end facing the tank wall.

[0016] Furthermore, the power unit is electromagnetically driven, and the rotor has uniformly distributed coils wound on it. Applying electricity to the coils generates magnetic poles on the rotor that rotate around its central axis.

[0017] The stator is made of magnetic material and generates a magnetic field with stable magnetic poles, which drives the rotor to rotate by the attraction and repulsion between the magnetic poles.

[0018] Furthermore, the power unit is driven by a high-pressure medium, the stator is provided with a plurality of chambers and a passage connecting the chambers and conveying the high-pressure medium, at least a portion of the rotor is located between the chambers and can rotate relative to the stator under the pressure of the high-pressure medium, and the high-pressure medium loses pressure after pushing the stator and flows from one chamber to another.

[0019] Preferably, the high-pressure medium includes high-pressure gas or high-pressure liquid.

[0020] The present invention also provides a control method applicable to the above-mentioned plant tissue culture vessel.

[0021] Furthermore, a standard value L0 for the indeterminate root is preset, and the length value L1 of the indeterminate root is detected every time interval t. The size of L1 is compared with that of L0. If L1 is greater than L0, the power unit is activated to cut the indeterminate root.

[0022] Furthermore, the detection of the length value L1 of the adventitious root includes: collecting several adventitious root samples from the tank, obtaining the actual length of the adventitious root samples, controlling the calculation of the average value of the actual length, and obtaining the length value L1 of the adventitious root.

[0023] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0024] 1. The power unit, which magnetically drives the shearing unit, eliminates the need for the traditional shaft connection structure. This eliminates the need for a sealing structure between the plant tissue culture tank and the power unit, improving the sealing of the tank wall and completely preventing oil or microorganisms from entering the tank. This ensures that the nutrient solution inside the tank remains sterile for a long time, providing a stable and reliable environment for adventitious root growth. Consequently, it greatly increases the yield and quality of adventitious roots within the same culture cycle.

[0025] 2. By setting the rotor of the power unit to a long rod shape, more parts can be installed on the rotor. One end is used to receive the driving force of the stator, and the other end is used to approach or extend into the shearing part, thereby increasing the attraction force of the shearing part. In addition, the simple transmission part can be used to reduce the size of the power unit.

[0026] 3. By regularly testing adventitious roots, the length of adventitious roots can be pruned and controlled, which solves the problem of adventitious roots easily tangling and causing necrosis. Furthermore, by pruning adventitious roots, callus tissue is induced to form, keeping adventitious roots in a high-speed growth state and increasing the yield of adventitious roots.

[0027] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0028] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0029] Figure 1 This is a schematic diagram of the bottom of a plant tissue culture vessel according to the present invention;

[0030] Figure 2 This is a schematic diagram of the cut structure of a plant tissue culture vessel according to the present invention;

[0031] Figure 3 This is a schematic diagram of the shearing section of a cutting structure according to the present invention.

[0032] Among them: 11. Tank wall; 20. Tank bottom; 90. Tank cavity; 31. Spacer; 311. Protruding column; 312. Flange edge; 32. Shearing part; 321. Pin shaft; 322. Blade assembly; 323. First blade; 324. Second blade; 325. Rotating end; 326. Blade end; 327. Closed side; 328. Unfolding side; 33. Transmission part; 331. Discharge section; 332. Drive section; 333. Discharge port; 34. Power unit; 35. Flow guide; 351. Inlet; 352. Outlet; 353. Circulation port.

[0033] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0035] In the description of this invention, it should be noted that the terms "inner" and "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0036] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," "contact," and "communication" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0037] In this invention, plant tissues include callus, adventitious buds, adventitious roots, etc., induced from isolated plant tissues or cells.

[0038] A culture device for large-scale cultivation of adventitious roots is provided with at least two plant tissue culture tanks of different volumes.

[0039] Take a culture device with two plant tissue culture vessels as an example. For ease of distinction, the smaller plant tissue culture vessel is called an adventitious root seed vessel, and the larger plant tissue culture vessel is called an adventitious root fermentation vessel.

[0040] The plant tissue culture jars are all made of stainless steel. The height-to-diameter ratio of the jars is 1:1. The adventitious root seed jars have a volume of 200L, while the adventitious root fermentation jars have volumes of 1000L and 1500L.

[0041] Plant tissue culture vessels contain a nutrient solution, and adventitious roots grow much faster than native roots in these vessels. After a period of growth, a large number of adventitious roots will become entangled and even clump together, which will seriously affect the production rate and quality of adventitious roots. Therefore, it is necessary to prune adventitious roots regularly to accurately control their length, ensure growth rate, and improve yield and quality.

[0042] Example 1

[0043] like Figure 1 As shown, this embodiment introduces a plant tissue culture vessel. The plant tissue culture vessel is equipped with a cutting structure having a shearing part 32 and a power part 34. The power part 34 can drive the shearing part 32 to cut adventitious roots. The power part 34 and the shearing part 32 are not directly connected.

[0044] Specifically, the shearing section 32 is disposed within the cavity 90 of the plant tissue culture vessel and is used for shearing adventitious roots. The power unit 34 is disposed on the outside of the plant tissue culture vessel. Furthermore, the central axis of the power unit 34 coincides with the central axis of the shearing section 32.

[0045] The power unit 34 is equipped with a rotor that can magnetically drive the shearing part 32 to rotate through the tank wall 11 to open and close the cut. Specifically, a magnetic suction element made of magnetic material is fixedly installed on the rotor. The magnetic suction element can attract the shearing part 32 through the tank wall 11 of the plant tissue culture tank and rotate with the rotor around the central axis of the power unit 34.

[0046] In this embodiment, the power unit 34 is set to drive the shearing unit 32 with magnetic force, eliminating the traditional shaft connection structure. This eliminates the need for a sealing structure between the plant tissue culture tank and the power unit 34, which not only improves the sealing performance of the plant tissue culture tank wall 11, but also completely prevents oil or microorganisms from the power unit 34 from entering the interior of the plant tissue culture tank. This ensures that the nutrient solution inside the plant tissue culture tank can maintain a sterile environment for a long time, providing a stable and reliable environment for the growth of adventitious roots. Consequently, it greatly improves the yield and quality of adventitious roots within the same culture cycle.

[0047] like Figure 3 As shown, in another embodiment of the present invention, a shearing section 32 that can be used in plant tissue culture tanks is described.

[0048] The shearing section 32 includes a first blade 323 and a second blade 324. The first blade 323 and the second blade 324 are disposed close together and connected by a pivot perpendicular to the contact surface. Both the first blade 323 and the second blade 324 can rotate freely around the pivot, and the opposite sides of the two blades form continuously opening and closing slits, which can be used to shear adventitious roots.

[0049] Preferably, the shearing part 32 includes a fixed first blade 323 and a second blade 324 that can rotate relative to the first blade 323 to form a cut.

[0050] The power unit 34 is equipped with a magnetic attractor that can rotate around its central axis. The central axis of the power unit 34 is aligned with the central axis of the second blade 324. The magnetic attractor uses a magnetic field to hold the second blade 324 in place through the can wall 11, causing it to rotate together.

[0051] In this embodiment, the shearing part 32 is composed of two blades that can rotate relative to each other, and the two blades form a continuously opening and closing slit. The adventitious root is cut through the slit to make the adventitious root cut flush, avoiding dragging and tearing of the adventitious root, improving the success rate of adventitious root cutting, preventing the adventitious root from dying after being cut, and improving the yield and quality of adventitious roots.

[0052] like Figure 3 As shown, in another embodiment of the present invention, a power unit 34 is described. The power unit 34 includes a stator and a rotor that can be driven by the stator. The stator is fixed to the outer surface of the tank wall 11. The rotor and stator are fitted together, with a gap between them. Introducing energy into the rotor can generate a force exerted by the stator on the rotor. This force produces a torque effect relative to the central axis, thereby driving the rotor to rotate indefinitely around the stator.

[0053] Understandably, energy sources include power sources, high-pressure gases or high-pressure liquids, and so on.

[0054] In this embodiment, the power unit 34 is configured with a stator and a rotor to provide torque, which reduces the number of parts in the power unit 34, improves the functional conversion efficiency of the power unit 34, and also reduces the noise of the power unit 34 during operation.

[0055] Furthermore, in another embodiment of the present invention, an optimized power unit 34 is described. In this power unit 34, the rotor is configured as an annular shape. The stator is configured as a cylinder, and the diameter of the stator is smaller than the diameter of the annular hole in the rotor. The rotor is fitted onto the outer periphery of the stator.

[0056] The magnetic attractor is fixed in the rotor on the end face facing the tank wall 11. An easily attracted iron block is provided on the outer periphery of the second blade 324. The magnetic attractor rotates along the normal of the second blade 324 against the tank wall 11 and attracts the second blade 324 to rotate together.

[0057] In this embodiment, the rotor of the power unit 34 is optimized into a circular ring shape, so that the stator can apply force to the rotor in a balanced manner, which is beneficial to maintaining the stability of the rotation process, increasing the rotor speed and the torque of the power unit 34.

[0058] Furthermore, in another embodiment of the present invention, an optimized power unit 34 is described. The power unit 34 also includes a stator and a rotor. The stator is configured in a ring shape and is made of a strongly magnetic material. The middle portion of the stator has a through hole extending through both ends along its central axis.

[0059] The rotor is configured as a long rod. The rotor is fitted into a through-hole in the stator and can rotate freely within the through-hole. The magnetic attractor is fixed within the rotor at one end facing the tank wall 11.

[0060] Preferably, a coil is wound around one end of the rotor, and the other end is connected to the magnetic attractor. The magnetic attractor is cylindrical. The second blade 324 is also provided with a cylindrical transmission part 33, one end of which is connected to the second blade 324, and the other end is sleeved on the outer periphery of the magnetic attractor.

[0061] In this embodiment, the rotor of the power unit 34 is configured as a long rod shape, which allows for the installation of more parts on the rotor. One end is used to receive the driving force of the stator, and the other end is used to approach or extend into the shearing part 32, thereby increasing the force that attracts the shearing part 32. Furthermore, the simple transmission part 33 can be used, which helps to reduce the volume of the power unit 34.

[0062] Furthermore, in another embodiment of the present invention, an electrically driven power unit 34 is described.

[0063] The power unit 34 includes a stator and a rotor. Multiple coils are wound on the rotor. Applying electricity to the coils generates magnetic poles on the rotor that rotate about its central axis.

[0064] Specifically, multiple coils are evenly distributed around the rotor, and only one coil is energized. Then, by switching the energization between the coils in a clockwise or counterclockwise sequence, magnetic poles that continuously change direction around the rotor's central axis are generated.

[0065] The stator is made of magnetic material and generates a stable magnetic field. By energizing the rotor, rotating magnetic poles are generated, which in turn drive the rotor to rotate relative to the stator through the attraction and repulsion between the magnetic poles.

[0066] In this embodiment, coils are provided on the rotor, and magnetic poles are generated by energizing the rotor. Then, by utilizing the property that like poles repel and unlike poles attract, the energized coils are switched synchronously with the rotation of the rotor, so that the magnetic poles on the rotor are always inconsistent with the rotor's magnetic poles, thereby continuously receiving the torque applied to the rotor by the stator, which greatly improves the functional conversion efficiency of the power unit 34.

[0067] Further preferably, in another embodiment of the present invention, a power unit 34 that can be driven by a high-pressure medium is provided. The power unit 34 includes a stator and a rotor, the high-pressure medium flows between the stator and the rotor and does work on the rotor, causing the rotor to rotate relative to the stator.

[0068] Specifically, the stator is provided with passages for conveying high-pressure media and several chambers. The passages connect all the chambers and allow the high-pressure media to be conveyed between the chambers. At least a portion of the rotor is located between the chambers and blocks the passages. The high-pressure media flows from the passages to adjacent chambers, exerting a force on the rotor. The rotor, pushed by the high-pressure media, rotates relative to the stator.

[0069] The high-pressure medium pushes the stator from one chamber to another, losing some of its pressure. This pressure is then converted into the rotor's kinetic energy.

[0070] Preferably, the high-pressure medium includes high-pressure gas or high-pressure liquid.

[0071] In this embodiment, the power unit 34 is provided with a structure that can convert high-pressure potential energy into kinetic energy, so that it can be driven by high-pressure gas or high-pressure liquid, enabling the power unit 34 to be used in flammable and explosive environments, thus expanding the application range of the power unit 34.

[0072] Furthermore, in another embodiment of the present invention, a control method for a plant tissue culture vessel equipped with the aforementioned power unit 34 is described. The control method controls the start and stop of the power unit 34 by determining whether the length value of the adventitious roots meets set conditions, thereby pruning the adventitious roots.

[0073] Specifically, a standard value L0 for adventitious roots growing in a plant tissue culture vessel is preset. The length value L1 of the adventitious root is detected every time interval t, and the size of L1 is compared with L0. If L1 is greater than L0, the power unit 34 is activated to cut the adventitious root.

[0074] In this embodiment, the length of adventitious roots is pruned and controlled by periodically detecting them, which solves the problem of adventitious roots easily tangling and causing necrosis. Furthermore, pruning adventitious roots induces the formation of callus tissue, keeping them in a high-speed growth state and increasing the yield of adventitious roots.

[0075] Furthermore, in another embodiment of the present invention, an optimized control method is described.

[0076] A standard value L0 is preset for adventitious roots growing in plant tissue culture tanks. Several adventitious root samples are collected from the tank at time intervals t to obtain the actual length of the adventitious root samples.

[0077] The average value of the actual length is calculated to obtain the length value L1 of the adventitious root. The size of L1 is compared with that of L0. If L1 is greater than L0, the power unit 34 is activated to cut the adventitious root.

[0078] In this embodiment, by obtaining the average length of adventitious roots to control the cycle of adventitious root pruning, the operating status of the plant tissue culture tank can be controlled more precisely, providing more stable production conditions for adventitious roots. Furthermore, by pruning adventitious roots to induce the formation of callus tissue, the adventitious roots are always kept in a high-speed growth state, which improves the cultivation efficiency and quality of adventitious roots.

[0079] Example 2

[0080] like Figure 2 As shown in the illustration, this embodiment describes some cutting structures for plant tissue culture vessels. The cutting structure is detachably connected to the vessel wall 11 of the plant tissue culture vessel.

[0081] Specifically, the cutting structure includes a shearing section 32 and a power section 34, which drives the shearing section 32 to cut adventitious roots. The power section 34 and the shearing section 32 are not directly connected; the shearing section 32 is located inside the cavity 90 of the plant tissue culture vessel, while the power section 34 is located outside the plant tissue culture vessel. The power section 34 and the shearing section 32 are separated by the vessel wall 11 of the plant tissue culture vessel.

[0082] Specifically, the plant tissue culture vessel is also equipped with a spacer 31. The vessel wall 11 of the plant tissue culture vessel has a circular mounting hole, and the outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole on the vessel wall 11.

[0083] One side of the spacer 31 is connected to the shearing part 32, and the other side is connected to the power unit 34, serving to separate the shearing part 32 and the power unit 34 inside and outside the plant tissue culture vessel. The shearing part 32 is installed in the spacer 31 on the side facing the vessel cavity 90, and the power unit 34 is installed on the spacer 31 on the side facing the outside of the plant tissue culture vessel. By making the cross-section of the spacer 31 larger than the cross-section of the shearing part 32, the shearing part 32 can be directly inserted through the mounting hole, thereby allowing the cutting structure to be easily and quickly installed on the plant tissue culture vessel.

[0084] The spacer 31 has various styles. In some embodiments of the present invention, the spacer 31 is annular and has an annular groove. When the spacer 31 is annular, the middle part of the spacer 31 is a thin-walled plate and an annular groove is formed by recessing into one side of the plate surface on the outer circumference of the thin-walled plate.

[0085] In other embodiments, the spacer 31 is cylindrical and has a cylindrical groove.

[0086] The cavity opening of the spacer 31 is always located on the end face of the spacer 31. In some embodiments, the spacer 31 is integral with the tank wall 11 and is formed by the tank wall 11 recessing into the cavity of the tank body. In this case, the groove opening of the spacer 31 is formed on the surface of the tank body.

[0087] In other embodiments, the spacer 31 is a separate part that is separable from the tank wall 11. The spacer 31 can be detachably mounted to the tank wall 11 by fasteners, or it can be fixed to the tank wall 11 by means of bonding or welding.

[0088] In this embodiment, a cutting structure capable of cutting adventitious roots is provided in the plant tissue culture vessel. At the same time, a spacer 31 is provided between the cutting part 32 and the power part 34, so that the cutting structure is distributedly installed inside and outside the vessel wall 11 of the plant tissue culture vessel. This allows the length of adventitious roots to be controlled by the cutting structure to maintain and promote the cultivation efficiency of adventitious roots, and also prevents microorganisms or impurities from entering the plant tissue culture vessel through the cutting structure, thereby improving the sealing of the plant tissue culture vessel.

[0089] like Figure 2 As shown, in another embodiment of the present invention, a spacer 31 with a cutting structure is introduced. This spacer 31 enables the power unit 34 to better attract and drive the cutting unit 32 to perform the cutting action from one side of the spacer 31, which simplifies the structure of mutual attraction between the cutting unit 32 and the power unit 34, and increases the attraction force of the power unit 34 on the cutting unit 32.

[0090] Specifically, the spacer 31 protrudes from the surface of the plant tissue culture vessel wall 11 toward the vessel cavity 90 along the vertical direction of the vessel wall 11. Viewed from the outside of the plant tissue culture vessel wall 11, corresponding to the position where the spacer 31 protrudes into the vessel cavity 90, the spacer 31 forms a groove on the other side.

[0091] Specifically, the shearing part 32 is connected to the outer convex surface of the spacer 31, and the power unit 34 is installed in the groove. Preferably, the shearing part 32 is provided with a component that is sleeved on the outer periphery of the spacer 31 and can rotate relative to the spacer 31. The power unit 34 attracts and drives the component from the other side of the spacer 31 through the spacer 31, thereby driving the shearing part 32 to cut the adventitious root.

[0092] In this embodiment, by optimizing the shape of the spacer 31 so that the spacer 31 protrudes into the cavity 90 of the tank, the attraction and driving effect of the power unit 34 on the shearing unit 32 can be enhanced, ensuring that the cutting structure can better cut the adventitious roots, avoid the adventitious roots from tangling and clumping, and thus promote the faster growth of the adventitious roots.

[0093] like Figure 2 As shown, in another embodiment of the present invention, a spacer 31 with a different cutting structure is introduced. Unlike the spacer 31 in the previous embodiment, the outer circumference of this spacer 31 is provided with an annular groove recessed along its central axis, and the middle part of the spacer 31 is located between the bottom and top of the groove in the direction of the central axis of the annular groove.

[0094] Preferably, the middle part of the spacer 31 is flush with the top of the tank, that is, the middle part of the spacer 31 is flush with the surface of the tank wall 11 of the plant tissue culture tank.

[0095] Specifically, when the spacer 31 is installed on the plant tissue culture jar, the annular groove of the spacer 31 extends from the surface of the jar wall 11 to the jar cavity 90 along the vertical direction of the jar wall 11, and the groove opening of the annular groove is located on the surface of the jar wall 11 of the plant tissue culture jar, so that the spacer 31 forms an annular structure with an annular groove.

[0096] Preferably, the spacer 31 protrudes into the cavity 90 of the tank body from its middle portion, forming a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 31 so that it protrudes into the cavity 90 of the tank body, the attractive force and driving effect of the power unit 34 on the shearing unit 32 can be enhanced, ensuring that the cutting structure can better shear the adventitious roots, avoid the adventitious roots from tangling and clumping, and thus promote the faster growth of the adventitious roots.

[0097] like Figure 2As shown, in another embodiment of the present invention, a spacer 31 is detachably mounted on a plant tissue culture vessel. Mounting holes are provided on the vessel wall 11. If the spacer 31 is annular, it is fixed to the mounting hole by embedding its annular groove into the vessel cavity 90; if the spacer 31 is barrel-shaped, it is fixed to the mounting hole by embedding its bottom into the vessel cavity 90.

[0098] Specifically, a mounting hole is made in the wall 11 of the plant tissue culture vessel, and the outer circumferential surface of the spacer 31 is set to be cylindrical and the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole on the vessel wall 11.

[0099] In this embodiment, the spacer 31 is connected to the shearing part 32 on one side and to the power unit 34 on the other side, separating the shearing part 32 and the power unit 34 inside and outside the plant tissue culture vessel. The shearing part 32 is installed inside the vessel cavity 90, and the power unit 34 is installed outside the plant tissue culture vessel. The shearing part 32 can be directly inserted into the vessel cavity 90 of the plant tissue culture vessel through the mounting hole, thereby allowing the cutting structure to be easily and quickly installed on the plant tissue culture vessel.

[0100] like Figure 1 As shown, in another embodiment of the present invention, the installation position of a plant tissue culture vessel and a spacer 31 is described.

[0101] The plant tissue culture vessel is configured in the shape of a body of revolution, with its central axis aligned vertically. The vessel wall 11 includes at least a portion extending downwards and gradually sloping towards the central axis of the plant tissue culture vessel. This portion forms an inverted cone shape.

[0102] Preferably, the tank wall 11 also includes a cylindrical portion parallel to the central axis. The cylindrical portion and the inverted conical portion are connected to form the whole plant tissue culture tank, with the inverted conical portion located below and serving as the tank bottom 20. The spacer 31 is disposed on the inverted conical portion, that is, the spacer 31 is disposed on the tank bottom 20, thereby the cut structure is distributed on the tank bottom 20 of the plant tissue culture tank.

[0103] In this embodiment, the plant tissue culture tank has an inverted conical bottom 20, which reduces the pressure of the nutrient solution on the adventitious roots and promotes their growth. Furthermore, the inverted conical bottom 20 helps to gather the adventitious roots suspended in the nutrient solution, allowing the spacer 31 and the cutting structure to be installed on the inverted conical bottom 20, ensuring that the cutting structure can efficiently and effectively shear the adventitious roots.

[0104] like Figure 3As shown, in some other embodiments of the present invention, a cutting section 32 of a cutting structure is described. The cutting section 32 includes a first blade 323 and a second blade 324. The first blade 323 and the second blade 324 are disposed close together and connected in series by a rotating shaft perpendicular to the contact surface. Both the first blade 323 and the second blade 324 can rotate freely about the rotating shaft, and the opposite sides of the two blades form continuously opening and closing slits, which can be used to cut adventitious roots.

[0105] Another preferred connection method is that the first blade 323 is fixedly connected, and the second blade 324 rotates relative to the first blade 323 around the pivot.

[0106] More preferably, the first blade 323 is fixedly connected to the spacer 31, and the second blade 324 is sleeved on the spacer 31 and can rotate relative to the central axis of the spacer 31. Meanwhile, the power unit 34 is equipped with a rotor that performs circular motion within the groove. A magnetic attractor is mounted on the rotor, which can attract the second blade 324 through the spacer 31.

[0107] In this embodiment, the shearing part 32 is composed of two blades that can rotate relative to each other, and the two blades form a continuously opening and closing slit. The adventitious root is cut through the slit to make the adventitious root cut flush, avoiding dragging and tearing of the adventitious root, improving the success rate of adventitious root cutting, preventing the adventitious root from dying after being cut, and improving the yield and quality of adventitious roots.

[0108] like Figure 2 As shown, in another embodiment of the present invention, a cutting portion 32 capable of cutting out adventitious roots of a fixed length is introduced.

[0109] The shearing section 32 includes at least two blade sets 322 spaced apart in the axial direction. Each blade set 322 includes a first blade 323 and a second blade 324, with their sides in contact. The spacing between adjacent blade sets 322 is between 10 mm and 15 mm.

[0110] Specifically, in the blade assembly 322, all the first blades 323 are parallel to each other and fixed to the pivot, and all the second blades 324 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 323. The second blades 324 rotate relative to the first blades 323 to form continuously opening and closing cuts.

[0111] In this way, when the second blade 324 is driven to rotate relative to the first blade 323, the adjacent blade groups 322 simultaneously cut the adventitious roots that have entered the shearing section 32, so that the length of the cut adventitious roots is consistent with the interval between the adjacent blade groups 322.

[0112] In this embodiment, the shearing part 32 is provided with multiple sets of blades, and the blade sets 322 are arranged at fixed intervals, so that adventitious roots can be cut to obtain adventitious roots of uniform length, so that the adventitious roots in the plant tissue culture tank have the same growth state, which facilitates the periodic adjustment of nutrient content of nutrient solution and the pruning of adventitious roots, promotes nutrient solution circulation in plant tissue culture tank, maintains stable growth conditions, and improves cultivation efficiency and the quality of adventitious roots.

[0113] like Figure 2 As shown, in another embodiment of the present invention, a cutting structure for a plant tissue culture vessel is described. The cutting structure further includes a transmission part 33 between the cutting part 32 and the power part 34.

[0114] Specifically, the transmission part 33 is cylindrical and is sleeved on the spacer 31. The inner cylindrical surface of the transmission part 33 and the outer peripheral surface of the spacer 31 can be slidably connected or rotatably connected and connected together by bearings. In particular, the transmission part 33 can rotate around the spacer 31. The transmission part 33 is connected to the second blade 324 and rotates synchronously, and the power part 34 drives the shearing part 32 to cut the adventitious roots through the transmission part 33.

[0115] like Figure 3 As shown, in another embodiment of the present invention, a flow guide 35 for a trimmed structure is introduced. A trimmed structure is provided inside the plant tissue culture vessel. To better promote the circulation of adventitious roots within the vessel cavity 90 and to avoid the trimmed structure repeatedly trimming a portion of the adventitious roots, a flow guide 35 for guiding liquid flow is also provided on the trimmed structure.

[0116] Specifically, the flow guide 35 is a cylindrical thin-walled tube with cavities extending through both ends along its central axis.

[0117] A flow guide shroud 35 is fitted around the outer periphery of the shearing section 32. One end of the flow guide shroud 35 protrudes from the shearing section 32 and has an inlet 351 on its end face. The inlet 351 is parallel to the circular surface formed by the rotation of the second blade 324. The other end of the flow guide shroud 35 extends toward the tank wall 11 and has an outlet 352 on its end face. The outlet 352 is fitted around the outer periphery of the transmission section 33, so that the flow guide shroud 35 at least partially covers the transmission section 33.

[0118] In this embodiment, by setting a flow guide 35 on the outer periphery of the shearing part 32, the circulation flow area of ​​the nutrient solution in the plant tissue culture tank is increased, which promotes the large-scale circulation of adventitious roots in the tank cavity 90. This avoids the adventitious roots in the vicinity of the shearing part 32 from circulating only in a small range due to the influence of the shearing part 32, ensuring that the plant tissue culture tank is in a good circulation state, thereby ensuring that all adventitious roots can be pruned.

[0119] like Figure 1 As shown, in another embodiment of the present invention, a plant tissue culture vessel is introduced. This plant tissue culture vessel is provided with any of the cutting structures described in the above embodiments. Using this plant tissue culture vessel to cultivate adventitious roots promotes the circulation of nutrient solution within the vessel, provides stable growth conditions for adventitious roots, and improves the cultivation efficiency and quality of adventitious roots.

[0120] Example 3

[0121] like Figure 2 As shown, in one embodiment of the present invention, a cutting section 32 of a cutting structure is introduced.

[0122] The shearing section 32 includes a pin 321, a first blade 323, and a second blade 324 that is in contact with the first blade 323. The first blade 323 and the second blade 324 are connected together by the pin 321. The pin 321 is perpendicular to the plane of the first blade 323 and the second blade 324.

[0123] In one connection method: the first blade 323 is fixed to the pin 321. The second blade 324 is rotatable relative to the first blade 323 about the pin 321 as a central axis. The opposite sides of the two blades form continuously opening and closing slits, which can be used to cut adventitious roots.

[0124] Specifically, as the second blade 324 rotates, the edges of the two blades change from an interleaved state to an overlapping state, forming continuously opening and closing cuts on the opposite sides of the two blades.

[0125] In this embodiment, the shearing part 32 includes two blades that can rotate relative to each other, with the sides of the two blades touching each other. This allows the overlapping and intersecting edges of the two blades to form continuously opening and closing cuts. The pressure exerted on the adventitious roots by the cuts ensures that the adventitious roots are completely cut off, avoiding the problem of uncut adventitious roots or even entanglement with the shearing part 32 causing damage to the cutting structure. It also ensures the flatness of the adventitious root cuts, preventing the adventitious roots from dying after being cut, which is beneficial to improving the growth rate and quality of the adventitious roots.

[0126] like Figure 2 As shown, in one embodiment of the present invention, a cutting portion 32 capable of cutting out adventitious roots of the same length is introduced.

[0127] The shearing section 32 includes multiple blade sets 322 connected in series on the pin 321. The multiple blade sets 322 are distributed at equal intervals along the axial direction of the pin 321, forming a multi-layer structure. Each blade set 322 is composed of two first blades 323 and two second blades 324 mating together.

[0128] Specifically, in the blade assembly 322, all the first blades 323 are parallel to each other and fixed to the pivot, and all the second blades 324 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 323. The second blades 324 rotate relative to the first blades 323 to form continuously opening and closing cuts.

[0129] In this way, when the second blade 324 is driven to rotate relative to the first blade 323, the adjacent blade groups 322 simultaneously cut the adventitious roots that have entered the shearing section 32, so that the length of the cut adventitious roots is consistent with the interval between the adjacent blade groups 322.

[0130] In this embodiment, the shearing part 32 is provided with multiple sets of blades, and the blade sets 322 are arranged at fixed intervals, so that adventitious roots can be cut to obtain adventitious roots of uniform length, so that the adventitious roots in the plant tissue culture tank have the same growth state, which facilitates the periodic adjustment of nutrient content of nutrient solution and the pruning of adventitious roots, promotes nutrient solution circulation in plant tissue culture tank, maintains stable growth conditions, and improves cultivation efficiency and the quality of adventitious roots.

[0131] like Figure 2 As shown, in another embodiment of the present invention, a shearing section 32 provided with two blade sets 322 is described.

[0132] Two first blades 323 are spaced apart and fitted onto the pin 321 and are arranged parallel to each other. Two second blades 324 respectively abut against the outer side of the first blades 323 opposite to the space.

[0133] The two second blades 324 are arranged in parallel and connected at their ends along their length to form a frame structure. The second blades 324 can be embedded inside the first blade 323 or can be wrapped around the first blade 323 from the outside.

[0134] Preferably, the second blade 324 covers the outside of the first blade 323, that is, it is attached to the side of the first blade 323 that is opposite to the gap.

[0135] In this embodiment, the shearing part 32 is provided with two blade groups 322, and the second blades 324 are connected as a whole. It can be connected from either side of the shearing part 32 and the two second blades 324 can be driven to rotate simultaneously. This simplifies the structure of the shearing part 32, so that the shearing part 32 can not only cut out adventitious roots of the same length, but also keep the cuts of the two blade groups 322 opening and closing synchronously, thus improving the efficiency of trimming adventitious roots.

[0136] like Figure 3As shown, in another embodiment of the present invention, a second blade 324 of a shearing section 32 is described. The second blade 324 includes a rotating end 325 and a blade end 326.

[0137] One end of the blade end 326 is connected to the rotating end 325, and the other end extends along the diameter of the rotating end 325 and is suspended on the outer circumference to form a cantilever.

[0138] The rotating end 325 is sleeved on the pin 321. When the second blade 324 rotates relative to the first blade 323, the blade end 326 can rotate around the pin 321 to form a continuously opening and closing cut.

[0139] like Figure 3 As shown, in another embodiment of the present invention, a shearing section 32 capable of forming curved cuts is introduced. The first blade 323 and the second blade 324 have the same structure and shape, that is, both are composed of a rotating end 325 and a blade end 326.

[0140] The blade end 326 includes a closed side 327 for cutting and pressing and an extended side 328 opposite to the closed side 327. The closed side 327 and the extended side 328 extend radially from the rotating end 325 to the other end and gradually approach each other.

[0141] like Figure 3 As shown, in another embodiment of the present invention, a shearing section 32 capable of gathering adventitious roots is introduced. The two ends of the closed side 327 are located on the same diameter line. Furthermore, the closed side 327 extends along an arc from the rotating end 325 to the other end. Finally, an inwardly recessed notch is formed on the closed side 327 of the blade end 326.

[0142] When the second blade 324 rotates relative to the first blade 323, the cut gradually closes from both ends of the closed side 327 toward the notch.

[0143] Furthermore, the second blade 324 includes a plurality of blade ends 326 evenly distributed along the outer periphery of the rotating end 325. For example, three blade ends 326 are arranged at 120° intervals on the outer periphery of the rotating end 325. Four blade ends 326 are arranged at 90° intervals on the outer periphery of the rotating end 325.

[0144] Preferably, the second blade 324 has two blade ends 326 symmetrically distributed about the rotating end 325.

[0145] Specifically, the unfolded side 328 extends outward along an arc from the rotating end 325, and the closed side 327 also extends outward along an arc from the rotating end 325. Furthermore, the unfolded side 328 and the closed side 327 gradually converge at a point. The second blade 324 is S-shaped and consists of two blade ends 326 connected to the rotating end 325 and spaced 180° apart.

[0146] like Figure 3 As shown, in another embodiment of the present invention, a shear section 32 is provided that can promote the circulation of adventitious roots. The blade tip 326 gradually curves upward along the circumferential direction from the closed side 327 to the unfolded side 328 to form a curved surface.

[0147] Preferably, the blade end 326 is configured as a helical surface along the diameter direction of the rotating end 325.

[0148] In another embodiment, to better guide the adventitious roots to circulate within the culture vessel, the first blade 323 is elongated. Furthermore, the first blade 323 has pointed angles at both ends that are inclined to its diameter. The side of the pointed angle opposite to the closed side 327 is recessed inwards from the first blade 323.

[0149] like Figure 1 As shown, in another embodiment of the present invention, a cutting structure for a plant tissue culture vessel is described.

[0150] The plant tissue culture vessel is configured in the shape of a body of revolution, with its central axis aligned vertically. The vessel wall 11 includes at least a portion extending downwards and gradually sloping towards the central axis of the plant tissue culture vessel. This portion forms an inverted cone shape.

[0151] Preferably, the tank wall 11 also includes a cylindrical portion parallel to the central axis. The cylindrical portion and the inverted conical portion connect to form the entire plant tissue culture tank, with the inverted conical portion located below and serving as the tank bottom 20. A cutting structure is provided in the inverted conical portion, that is, the spacer 31 is provided at the tank bottom 20.

[0152] In this embodiment, the cutting structure located at the bottom 20 of the container enables rapid cutting of adventitious roots, resulting in clean cuts and uniform growth of the adventitious roots within the plant tissue culture container. This facilitates regular pruning of the adventitious roots, promotes nutrient solution circulation within the plant tissue culture container, maintains favorable growth conditions for the adventitious roots, and improves the efficiency and quality of adventitious root cultivation within the container.

[0153] Example 4

[0154] like Figure 2As shown, in one embodiment of the present invention, a cutting structure having a transmission part 33 is described. The cutting structure includes a power part 34 and a shearing part 32 rotatably connected to a pin 321. The power part 34 and the shearing part 32 are not directly connected. The shearing part 32 is disposed within the cavity 90 of the plant tissue culture vessel, while the power part 34 is disposed outside the plant tissue culture vessel. The power part 34 and the shearing part 32 are separated by the vessel wall 11 of the plant tissue culture vessel.

[0155] Specifically, the cutting structure also includes a transmission part 33 sleeved on the pin 321. The pin 321 is fixed to the inner side of the wall 11 of the plant tissue culture vessel. One end of the transmission part 33 is connected to the shearing part 32, and the other end is disposed near the power part 34. The power part 34 transmits torque to the transmission part 33 through magnetic force, and can drive the transmission part 33 to rotate around the pin 321.

[0156] In this embodiment, a transmission part 33 is added between the shearing part 32 and the power part 34. The transmission part 33 can greatly increase the distance that the power part 34 drives the shearing part 32, optimize the layout of the shearing part 32 in the plant tissue culture tank, and allow the shearing part 32 to be close to the area with the highest adventitious root density, thereby improving the efficiency of the cutting structure in cutting adventitious roots, reducing the opening time of the cutting structure, greatly improving the environment inside the plant tissue culture tank, and promoting the improvement of adventitious root quality and yield.

[0157] like Figure 2 As shown, in one embodiment of the present invention, a transmission part 33 connected to a shearing part 32 is introduced. The shearing part 32 includes a first blade 323 and a second blade 324. The first blade 323 and the second blade 324 are disposed close together and connected in series by a pin 321 perpendicular to the contact surface. Both the first blade 323 and the second blade 324 can rotate freely around the pin 321, and the opposite sides of the two blades form continuously opening and closing slits, which can be used to shear adventitious roots.

[0158] Another preferred connection method: the first blade 323 is fixedly connected to the pin 321. The second blade 324 can rotate around the pin 321. The transmission part 33 is cylindrical and sleeved on the pin 321, and the transmission part 33 is also connected to the second blade 324.

[0159] Furthermore, in another embodiment of the present invention, a transmission part 33 connected to the second blade 324 is provided. The end of the second blade 324 in the longitudinal direction is connected to the inner circumferential surface of the transmission part 33.

[0160] Specifically, the transmission part 33 is cylindrical, with a through hole extending through both ends along its central axis. The second blade 324 is embedded and connected within the through hole of the transmission part 33. The two ends of the second blade 324 are connected to the inner surface of the through hole, and the length direction of the second blade 324 is parallel to the diameter of the through hole.

[0161] In this embodiment, the shearing part 32 is embedded in the through hole of the transmission part 33. The transmission part 33 is connected to the second blade 324 but isolated from the first blade 323. This allows the second blade 324 to rotate relative to the first blade 323 and pushes the adventitious root to circulate along the axis of the transmission part 33, thereby avoiding repeated cutting of the adventitious root, ensuring the length of the adventitious root, and improving the quality of the adventitious root.

[0162] like Figure 2 As shown, in another embodiment of the present invention, a cutting structure is described. The cutting structure includes a plurality of blade groups 322 connected in series on the pin 321. The plurality of blade groups 322 are distributed at equal intervals along the axial direction of the pin 321, forming a multi-layer structure. Each blade group 322 is composed of a first blade 323 and a second blade 324 mating together in pairs.

[0163] Preferably, the cutting structure is provided with two blade sets 322. Two first blades 323 are sleeved on the pin 321 in parallel and spaced apart, and two second blades 324 are arranged parallel to each other and sandwiched from the side of the first blades 323 away from the spaced distance.

[0164] Specifically, in the blade assembly 322, all the first blades 323 are parallel to each other and fixed to the pivot, and all the second blades 324 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 323. The second blades 324 rotate relative to the first blades 323 to form continuously opening and closing cuts.

[0165] In this way, when the second blade 324 is driven to rotate relative to the first blade 323, the adjacent blade groups 322 simultaneously cut the adventitious roots that have entered the shearing section 32, so that the length of the cut adventitious roots is consistent with the interval between the adjacent blade groups 322.

[0166] In this embodiment, the shearing part 32 is provided with multiple sets of blades, and the blade sets 322 are arranged at fixed intervals, so that adventitious roots can be cut to obtain adventitious roots of uniform length, so that the adventitious roots in the plant tissue culture tank have the same growth state, which facilitates the periodic adjustment of nutrient content of nutrient solution and the pruning of adventitious roots, promotes nutrient solution circulation in plant tissue culture tank, maintains stable growth conditions, and improves cultivation efficiency and the quality of adventitious roots.

[0167] like Figure 2As shown, in another embodiment of the present invention, a cutting structure capable of quick assembly and disassembly is described. The cutting structure further includes a spacer 31 with a cylindrical outer surface. The pin 321 is fixed to the top surface of the spacer 31, and the transmission part 33 is at least partially sleeved on the spacer 31.

[0168] Specifically, the plant tissue culture vessel wall 11 has a circular mounting hole, and the outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole on the vessel wall 11.

[0169] One side of the spacer 31 is connected to the shearing part 32, and the other side is connected to the power unit 34, serving to separate the shearing part 32 and the power unit 34 inside and outside the plant tissue culture vessel. The shearing part 32 is installed in the spacer 31 on the side facing the vessel cavity 90, and the power unit 34 is installed on the spacer 31 on the side facing the outside of the plant tissue culture vessel. By making the cross-section of the spacer 31 larger than the cross-section of the shearing part 32, the shearing part 32 can be directly inserted through the mounting hole, thereby allowing the cutting structure to be easily and quickly installed on the plant tissue culture vessel.

[0170] In this embodiment, a spacer 31 is provided in the plant tissue culture tank, so that the cutting structure is dispersedly installed inside and outside the tank wall 11 of the plant tissue culture tank. This allows the length of adventitious roots to be controlled through the cutting structure to maintain and promote the cultivation efficiency of adventitious roots, and also prevents microorganisms or impurities from entering the plant tissue culture tank through the cutting structure, thereby improving the airtightness of the plant tissue culture tank.

[0171] like Figure 2 As shown, in another embodiment of the present invention, a cutting structure in which a cavity is formed in the transmission part 33 is described.

[0172] The shearing section 32 and the spacer 31 are spaced apart along the axial direction of the pin 321. The end face of the shearing section 32 and the end face of the spacer 31 together with the inner circumferential surface of the transmission section 33 form a cavity for accommodating materials.

[0173] Preferably, to facilitate the outflow of adventitious roots from the transmission section 33, in another embodiment of the invention, the transmission section 33 is further provided with a discharge port 333.

[0174] The discharge port 333 is formed on the side wall of the transmission part 33 and extends radially through the transmission part 33. Multiple discharge ports 333 may be formed on the transmission part 33. The discharge ports 333 are evenly distributed on the side wall of the transmission part 33 in a circumferential direction.

[0175] Specifically, in the axial direction of the transmission part 33, the discharge port 333 is located between the shearing part 32 and the spacer 31. That is, the length of the discharge port 333 along the axial direction of the transmission part 33 is less than the interval length between the shearing part 32 and the spacer 31.

[0176] To facilitate the assembly of the transmission section 33, another embodiment of the present invention introduces a multi-segment spliced ​​transmission section 33. The transmission section 33 is configured to be formed by the docking of a discharge section 331 and a drive section 332.

[0177] Specifically, the discharge section 331 is sleeved on the pin 321. The discharge section 331 has a discharge port 333 on its side wall. The drive section 332 is sleeved on the outside of the spacer 31, and one end of the drive section 332 is flush with the top surface of the spacer 31.

[0178] Furthermore, to prevent the adventitious roots from being blocked by the side wall of the transmission section 33, and to accelerate the flow of the adventitious roots out of the discharge port 333 after being cut by the shearing section 32, a plurality of discharge ports 333 are provided and evenly distributed along the circumferential direction on the side wall of the discharge section 331. In particular, the end of the second blade 324 is connected to the side wall between adjacent discharge ports 333, thereby aligning the cut on the shearing section 32 with the discharge port 333.

[0179] like Figure 1 As shown, in another embodiment of the present invention, a plant tissue culture vessel is introduced. This plant tissue culture vessel is provided with any of the cutting structures described in the above embodiments. Using this plant tissue culture vessel to cultivate adventitious roots can improve the utilization rate of the cutting structure, increase cutting efficiency, reduce the opening time of the cutting structure, better maintain the growth cycle of adventitious roots, and improve the cultivation efficiency and quality of adventitious roots.

[0180] Example 5

[0181] like Figure 1 and Figure 2 As shown, in one embodiment of the present invention, a cut structure with a flow guide 35 is described.

[0182] The cutting structure includes a shearing section 32 with a first blade 323 and a second blade 324. The first blade 323 and the second blade 324 are disposed close together and connected by a pin 321 perpendicular to the contact surface. Both the first blade 323 and the second blade 324 can rotate freely around the pin 321, and the opposite sides of the two blades form continuously opening and closing slits, which can be used to cut adventitious roots.

[0183] Another preferred connection method: the first blade 323 is fixedly connected to the pin 321. The second blade 324 can rotate around the pin 321.

[0184] Specifically, when the cutting structure is activated, the first blade 323 and / or the second blade 324 rotate around the pin 321 to form a circular surface centered on and perpendicular to the pin 321. To prevent the already cut adventitious roots from circulating in a small area near the blades and causing repeated cutting, the cutting structure is also provided with a flow guide 35. The flow guide 35 is made of a thin-walled material and is fitted around the outer periphery of the shearing section 32.

[0185] Specifically, the deflector 35 is also provided with parallel inlet 351 and outlet 352. Furthermore, inlet 351 and outlet 352 are parallel to the circular surface formed when the blade rotates.

[0186] In this embodiment, by providing a flow guide 35 around the outer periphery of the shearing section 32, adventitious roots can be promoted to flow from one side of the shearing section 32 to the other side, increasing the circulation flow rate of adventitious roots. At the same time, it can also increase and promote the circulation flow area of ​​nutrient solution in the plant tissue culture tank, ensuring that the cut adventitious roots flow quickly from the shearing section 32 to other areas, avoiding the adventitious roots circulating in a small area near the shearing section 32, thereby reducing the rate of repeated cutting of adventitious roots, preventing the problem of uneven length of adventitious roots, and effectively improving the quality of adventitious roots in the culture tank.

[0187] like Figure 2 As shown, in another embodiment of the present invention, a cutting structure with a spacer 31 is described. The cutting structure also includes a spacer 31. One side of the spacer 31 is connected to the cutting part 32, and the other side is connected to the power part 34, serving to separate the cutting part 32 and the power part 34.

[0188] The plant tissue culture vessel has a circular mounting hole in its wall 11, and the outer circumference of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole in the vessel wall 11.

[0189] The shearing part 32 is installed in the spacer 31 on the side facing the cavity 90 of the tank, and the power unit 34 is installed on the spacer 31 on the side facing the outside of the plant tissue culture tank. The shearing part 32 can be directly inserted through the mounting hole by making the cross-section of the spacer 31 larger than the cross-section of the shearing part 32.

[0190] Specifically, the spacer 31 extends into the flow deflector 35 from the outlet 352 and connects to the shear section 32. Correspondingly, the inlet 351 of the flow deflector 35 is located on the side of the shear section 32 opposite to the spacer 31 in the axial direction of the shear section 32.

[0191] In this embodiment, the flow guide 35 is sleeved on the outside of the spacer 31. By disassembling and assembling the spacer 31, the flow guide 35 can be easily and quickly installed on the plant tissue culture tank, making the flow guide 35 easy to maintain and replace.

[0192] like Figure 2 As shown, in another embodiment of the present invention, a spacer 31 for a plant tissue culture vessel is described. The spacer 31 includes a cylindrical protrusion 311. A flow guide 35 is provided with a cylindrical through hole and fits on the outer peripheral surface of the protrusion 311. There is a gap between the through hole surface of the flow guide 35 and the outer surface of the protrusion 311. This gap forms an annular channel.

[0193] In another embodiment, the spacer 31 is further provided with a flange edge 312 extending outward from the end face edge of the protrusion 311. The flange edge 312 is annular. The flow guide 35 is connected to the flange edge 312.

[0194] Preferably, the flow guide 35 is connected to the outer ring edge of the flange 312.

[0195] In another embodiment, the flow guide 35 is configured as a cylindrical thin-walled tube. The flow guide 35 also has cavities extending through both ends along its central axis. The inlet 351 and the outlet 352 communicate with the cavities and are located at both ends of the thin-walled tube, respectively.

[0196] In another embodiment, a circulation port 353 is also provided on the side wall of the flow guide 35. When the flow guide 35 is fitted onto the cutting structure, one end of the circulation port 353 is aligned with the shearing part 32 along the axial direction of the cutting structure, and the other end is close to the outlet 352.

[0197] In this embodiment, by setting a flow guide 35 on the outer periphery of the shearing part 32, the circulation flow area of ​​the nutrient solution in the plant tissue culture tank is increased, which promotes the large-scale circulation of adventitious roots in the tank cavity 90. This avoids the adventitious roots in the vicinity of the shearing part 32 from circulating only in a small range due to the influence of the shearing part 32, ensuring that the plant tissue culture tank is in a good circulation state, thereby ensuring that all adventitious roots can be pruned.

[0198] like Figure 2 As shown, in another embodiment of the present invention, a transmission part 33 is introduced, which is fitted inside the flow guide 35. The cutting structure further includes a transmission part 33 between the shearing part 32 and the power part 34. The transmission part 33 is used to pull the shearing part 32. One end of the transmission part 33 is connected to the shearing part 32, and the other end is rotatably fitted onto the spacer 31 and located within the cavity of the flow guide 35.

[0199] Specifically, the transmission part 33 is cylindrical and is sleeved on the spacer 31. The inner cylindrical surface of the transmission part 33 and the outer peripheral surface of the spacer 31 can be slidably connected or rotatably connected and connected together by bearings.

[0200] Specifically, the transmission unit 33 is capable of rotating around the spacer 31. The transmission unit 33 is connected to and rotates synchronously with the second blade 324, and the power unit 34 drives the shearing unit 32 to cut the adventitious roots through the transmission unit 33.

[0201] In another embodiment, to avoid interference when the transmission part 33 is simultaneously fitted with the shearing part 32 and the spacer 31, to reduce fit tolerances and reduce assembly difficulty, the transmission part 33 is configured as a structure consisting of a discharge section 331 and a drive section 332.

[0202] The discharge section 331 can be configured as a thin rod shape. The discharge section 331 has through holes extending through both ends along its central axis. The drive section 332 is a cylindrical shape with one open end and the other closed end. The discharge section 331 and the drive section 332 coincide on their central axes and are connected to the closed end face of the drive section 332. At this time, the guide shroud 35 is sleeved on the outside of the transmission part 33, and an annular cavity is formed between the discharge section 331 and the guide shroud 35, which can be used to temporarily store the cut adventitious roots.

[0203] Furthermore, the shearing section 32 includes a pin 321, a first blade 323 fixed to the pin 321, and a second blade 324 rotatable around the pin 321. The second blade 324 has two blade ends 326. The discharge section 331 is sleeved on the pin 321 and its end is connected to the second blade 324. The blade ends 326 are suspended in the cavity between the discharge section 331 and the guide shroud 35, and the blade ends 326 have a helical surface.

[0204] Preferably, the arc length of the circulation port 353 in the circumferential direction of the guide shroud 35 corresponds to the angle between the arc length and the blade tip 326 of the first blade 323. This aligns the interval between the circulation port 353 and the blade tip 326 of the first blade 323, eliminating the resistance of adventitious root circulation.

[0205] Another preferred embodiment is that the discharge section 331 is cylindrical. Furthermore, the diameter of the outer circumference of the discharge section 331 is the same as the diameter of the inner surface of the drive section 332, so that it can be embedded in the drive section 332 for connection.

[0206] In another embodiment of the present invention, a plant tissue culture vessel having the above-described cutting structure is described.

[0207] The plant tissue culture vessel is also equipped with a flow guide 35 in its cutting structure to guide the flow of liquid. By setting the flow guide 35, the circulation of adventitious roots within the vessel cavity 90 can be better promoted, the circulation area of ​​nutrient solution in the plant tissue culture vessel can be increased and promoted, the cutting structure can be used to repeatedly cut the adventitious roots that have already been cut, the length of adventitious roots can be made uniform, the growth cycle of adventitious roots can be better maintained, and the quality and yield of adventitious roots can be improved.

[0208] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A plant tissue culture vessel, comprising a cutting structure having a shearing section and a power section; the vessel cavity is provided with a shearing section for shearing adventitious roots, characterized in that, A power unit is also provided outside the tank, which uses magnetic force to drive the shearing part to open and close the cut through the tank wall; The shearing section includes a pin, a first blade, and a second blade that is in contact with the first blade; the first blade and the second blade are connected together by the pin; the pin is perpendicular to the plane of the first blade and the second blade. The first blade is fixed to the pin, and the second blade can rotate relative to the first blade with the pin as the central axis. The opposite sides of the two blades form a continuously opening and closing cut. The second blade includes a rotating end and a blade end; the rotating end is sleeved on the pin, one end of the blade end is connected to the rotating end, and the other end extends along the diameter of the rotating end and is suspended on the outer circumference to form a cantilever shape; The blade tip includes a closed side for cutting and a spread side opposite to the closed side; The unfolded side extends outward from the rotating end along an arc, and the closed side also extends outward from the rotating end along an arc, with the unfolded and closed sides gradually converging at a point; the second blade is S-shaped and has two blade ends that are connected to the rotating end and set at 180° intervals. The blade tip gradually curves upwards along the circumferential direction from the closed side to the unfolded side to form a curved surface; the blade tip is set as a helical surface along the diameter direction of the rotating end; The cutting structure is also equipped with a flow guide, which is made of thin-walled material and is fitted around the outer periphery of the shearing part; the flow guide is also provided with parallel inlets and outlets, and the inlets and outlets are parallel to the circular surface formed when the blade rotates; The cutting structure also includes a partition sleeve; the shearing part is installed in the partition sleeve on the side facing the cavity of the tank, and the power part is installed on the partition sleeve on the side facing the outside of the plant tissue culture tank; the partition sleeve passes through the outlet of the flow guide and is connected to the shearing part. The flow guide is set as a cylindrical thin-walled tube; a circulation port is also opened on the side wall of the flow guide; when the flow guide is fitted onto the cutting structure, along the axial direction of the cutting structure, one end of the circulation port is aligned with the shearing part and the other end is close to the outlet.

2. A plant tissue culture vessel according to claim 1, characterized in that, The plant tissue culture vessel is designed as a rotating body with its central axis in a vertical direction. The vessel wall includes a portion extending downwards and gradually tilting towards the central axis of the plant tissue culture vessel, forming an inverted cone shape. The vessel wall also includes a cylindrical portion parallel to the central axis. The cylindrical portion and the inverted cone portion are connected to form the entire plant tissue culture vessel, with the inverted cone portion located below and serving as the bottom of the plant tissue culture vessel. A partition and a cutting structure are installed at the bottom of the inverted cone shape. The power unit is equipped with a magnetic suction device that can rotate around its central axis. The power unit and the second blade are arranged with their central axes coinciding. The magnetic suction device binds the second blade with a magnetic field through the can wall and rotates together.

3. A plant tissue culture vessel according to claim 2, characterized in that, The power unit also includes a stator and a rotor driven by the stator. The stator is fixed to the outer side of the tank wall, and the rotor is rotatably sleeved with the stator.

4. A plant tissue culture vessel according to claim 3, characterized in that, The rotor is configured in a ring shape and is fitted around the outer periphery of the stator, and the magnetic attractor is fixed in the rotor on the end face facing the tank wall.

5. A plant tissue culture vessel according to claim 3, characterized in that, The stator is configured as a ring shape and has through holes extending through both ends along its central axis. The rotor is configured as a long rod and is sleeved in the through holes. The magnetic attractor is fixed in the rotor at one end facing the tank wall.

6. A plant tissue culture vessel according to any one of claims 3-5, characterized in that, The power unit is electromagnetically driven, and the rotor has uniformly distributed coils wound on it. When electricity is applied to the coils, magnetic poles rotating around the rotor's central axis are generated. The stator is made of magnetic material and generates a magnetic field with stable magnetic poles, which drives the rotor to rotate by the attraction and repulsion between the magnetic poles.

7. A plant tissue culture vessel according to any one of claims 3-5, characterized in that, The power unit is driven by a high-pressure medium. The stator is provided with several chambers and a passage connecting the chambers and conveying the high-pressure medium. At least a part of the rotor is located between the chambers and can rotate relative to the stator under the pressure of the high-pressure medium. After the high-pressure medium pushes the stator, it loses pressure and flows from one chamber to another. High-pressure media include high-pressure gas or high-pressure liquid.

8. A method for controlling a plant tissue culture vessel, characterized in that, Control the operation of the plant tissue culture vessel as described in any one of claims 1-7.

9. The control method for a plant tissue culture vessel according to claim 8, characterized in that, A standard value L0 for the indeterminate root is preset. The length value L1 of the indeterminate root is detected every time interval t. The size of L1 is compared with that of L0. If L1 is greater than L0, the power unit is activated to cut the indeterminate root.

10. The control method for a plant tissue culture vessel according to claim 9, characterized in that, The detection of the length value L1 of the adventitious root includes: collecting several adventitious root samples from the tank, obtaining the actual length of the adventitious root samples, controlling the calculation of the average value of the actual length, and obtaining the length value L1 of the adventitious root.

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