Biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device

Abstract

This invention provides a biofilm bypass-enhanced anaerobic ammonia-oxidizing functional bacteria device. By controlling the conditions within the bypass enhancement device, anaerobic ammonia-oxidizing functional bacteria are enriched, forming a functional bacteria production workshop. These bacteria are then added to the biological treatment tank, improving the structure and function of the microorganisms, thereby enhancing wastewater treatment performance, increasing treatment capacity, improving effluent quality, and reducing operating costs. It is applicable to both above-ground and underground wastewater treatment plants, and can be used for non-stop retrofitting of existing water plant biological treatment units.

Description

Technical Field

[0001] This invention relates to the technical field of wastewater treatment, and more specifically, to a biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device. Background Technology

[0002] With the continuous expansion of urban areas and the increase in population, water pollution has become a key pollution problem that urgently needs to be addressed. In the operation of sewage treatment plants, the biological treatment tank is one of the core processes for removing pollutants, and the activated sludge in the biological treatment tank is a key factor affecting the treatment effect.

[0003] Compared to the main process improvements and technology of the biological treatment tank, improving performance through methods such as "bypass" and "side flow" is also a very important technical means.

[0004] Compared to the traditional "nitrifying bacteria-denitrifying bacteria" process, the anammox biochemical reaction process can save 100% of the carbon source, and the theoretical maximum nitrogen removal rate can reach 89%. An article in the journal *Science* points out that the application of anammox technology can promote the development of urban wastewater treatment plants towards energy self-sufficiency, thereby achieving sustainable development of urban wastewater treatment and reuse. However, its application in wastewater treatment plants faces many difficulties, especially in the main biological treatment process, where the abundance of anammox functional bacteria is relatively low. Furthermore, it is difficult to significantly increase the abundance of anammox functional bacteria in the main process due to limitations imposed by process parameters such as mixing state, dissolved oxygen, influent water quality, water temperature, and sludge age.

[0005] CN109851049A (Wastewater Treatment Unit and Its Usage and Application) was proposed by Guotou Xinkai Water Environment Investment Co., Ltd., and provides a method to improve the wastewater treatment effect by placing a biofilm reactor outside the biochemical tank, so that the wastewater in the biochemical tank flows through the biofilm reactor and undergoes further biochemical treatment.

[0006] Therefore, the application of anaerobic ammonia oxidation technology in the main process of biological treatment will help to further improve wastewater treatment and reuse. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the purpose of this invention is to provide a biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device.

[0008] According to the present invention, a biofilm bypass enhanced anaerobic ammonia oxidation functional bacteria device includes: a bypass enhancement device, a biological packing material, anaerobic ammonia oxidation functional bacteria, a side flow system, and a biochemical treatment main system;

[0009] The biological packing material is filled in a bypass enhancement device, in which anaerobic ammonia-oxidizing functional bacteria are domesticated. The flow measurement system is connected to the main biochemical treatment system, and the anaerobic ammonia-oxidizing functional bacteria enter the main biochemical treatment system through the flow measurement system.

[0010] Preferably, the bypass enhancement device adjusts and controls the parameters of flow rate, stirring, aeration, and added chemicals.

[0011] Preferably, the main biochemical treatment system includes, but is not limited to, AAO, AO, multi-stage AO, and SBR process types.

[0012] Preferably, the biological packing material is a carrier that promotes the growth of anaerobic ammonia-oxidizing bacteria.

[0013] Preferably, the side-flow system extracts a portion of the wastewater from the main biochemical treatment system and returns it to the reaction system of the main biochemical treatment system.

[0014] Preferably, the bypass enhancement device enriches anaerobic ammonia-oxidizing bacteria by controlling parameters such as influent flow rate, stirring, aeration, effluent flow rate, and the type and quantity of added chemicals.

[0015] Preferably, the anaerobic ammonia-oxidizing functional bacteria are domesticated and enriched through the following steps:

[0016] Step S1: Control the carbon source and NO3 inside and outside the bypass enhancement device. - -N concentration ratio is approximately COD / NO3 - -N = 6-9 mg / L, stir, reaction time 50-200 minutes;

[0017] Step S2: Add NH4 + -N, whose concentration is related to NO3 - The initial concentration ratio of -N is 0.8-1.5. Stir and react for 50-200 minutes.

[0018] Step S3: The mud-water mixture in the bypass enhancement device is discharged into the main biochemical treatment system;

[0019] Step S4: Repeat steps S1-S3.

[0020] Preferably, in step S1:

[0021] NO3 - -N substances are obtained from the main biological treatment system and from the effluent of the secondary sedimentation tank; COD is controlled by adding external agents and is obtained by extraction from the main biological treatment system and from the end of the anoxic tank.

[0022] Preferably, in step S2:

[0023] NH4 + -N substances are controlled by external reagents and are obtained by extraction from the main biochemical treatment system through a design.

[0024] Preferably, the bypass enhancement device and the side flow system are associated with the main biochemical treatment system by adjusting the influent flow rate and effluent flow rate parameters of the bypass enhancement device, and transport the domesticated and enriched anaerobic ammonia-oxidizing functional bacteria into the main biochemical treatment system.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. This invention can upgrade and transform the mainstream process of biological treatment tanks, thereby improving the removal performance of biological treatment tanks;

[0027] 2. This invention uses an external bypass component that operates independently of the main biochemical treatment system, making its process parameters easier to control, more feasible, and easier to operate.

[0028] 3. This invention can enrich anaerobic ammonia-oxidizing functional bacteria that grow slowly and are difficult to cultivate, and then promote their application in emerging and cutting-edge wastewater treatment technologies in engineering practice, thereby accelerating the industrialization of advanced technologies.

[0029] 4. The upgrade and modification process of this invention can be carried out without interrupting production, and can achieve rapid start-up and stable operation in a short period of time. Attached Figure Description

[0030] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0031] Figure 1 Schematic diagram of a biofilm bypass device for enhancing anaerobic ammonia-oxidizing bacteria;

[0032] Figure 2 This is a schematic diagram of steps 2 and 3 in Example 2;

[0033] Figure 3 This is a schematic diagram of step 5 in Example 2;

[0034] Figure 4 This is a schematic diagram of step 6 in Example 2;

[0035] Figure 5 This is a schematic diagram of step 7 in Example 2;

[0036] Figure 6 This is a schematic diagram of step 13 in Example 2. Detailed Implementation

[0037] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0038] Example 1:

[0039] According to the present invention, a biofilm bypass-enhanced anaerobic ammonia oxidation functional bacteria device includes: a bypass enhancement device, biological packing material, anaerobic ammonia oxidation functional bacteria, a sideflow system, and a biochemical treatment main system; the biological packing material is filled in the bypass enhancement device, the anaerobic ammonia oxidation functional bacteria are acclimated in the bypass enhancement device, the flow measurement system is connected to the biochemical treatment main system, and the anaerobic ammonia oxidation functional bacteria enter the biochemical treatment main system through the flow measurement system.

[0040] The bypass enhancement device adjusts and controls parameters such as flow rate, mixing, aeration, and added chemicals; the main biological treatment system includes, but is not limited to, AAO, AO, multi-stage AO, and SBR process types; the biological packing material serves as a carrier to promote the growth of anaerobic ammonia-oxidizing bacteria; the side flow system extracts a portion of the wastewater from the main biological treatment system and returns it to the reaction system of the main biological treatment system.

[0041] The bypass enhancement device enriches anaerobic ammonia-oxidizing bacteria by controlling parameters such as influent flow rate, agitation, aeration, effluent flow rate, and the type and quantity of added chemicals. The anaerobic ammonia-oxidizing bacteria are acclimatized and enriched through the following steps:

[0042] Step S1: Control the carbon source and NO3 inside and outside the bypass enhancement device. - -N concentration ratio is approximately COD / NO3 - -N = 6-9, stir, reaction time 50-200 minutes; NO3 - -N substances are obtained from the main biological treatment system and from the effluent of the secondary sedimentation tank; COD is controlled by adding external agents and is obtained by extraction from the main biological treatment system and from the end of the anoxic tank.

[0043] Step S2: Add NH4 + -N, whose concentration is related to NO3 - The initial concentration ratio of -N is NH4 + -N / NO3 - -N = 0.8-1.5, stir, reaction time 50-200 minutes; NH4 + -N substances are controlled by external reagents and are obtained by extraction from the main biochemical treatment system through a design.

[0044] Step S3: The mud-water mixture in the bypass enhancement device is discharged into the main biochemical treatment system;

[0045] Step S4: Repeat steps S1-S3.

[0046] The bypass enhancement device and side flow system are linked to the main biochemical treatment system by adjusting the influent and effluent flow parameters of the bypass enhancement device, and transport the domesticated and enriched anaerobic ammonia-oxidizing functional bacteria into the main biochemical treatment system.

[0047] Example 2:

[0048] This invention provides a biofilm bypass-enhanced anaerobic ammonia-oxidizing functional bacteria device and system. By controlling the conditions within the bypass enhancement device, anaerobic ammonia-oxidizing functional bacteria are enriched, forming a functional bacteria production workshop. These bacteria are then added to the biological treatment tank, improving the microbial structure and function, thereby enhancing wastewater treatment performance, increasing treatment capacity, improving effluent quality, and reducing operating costs. It is applicable to both above-ground and underground wastewater treatment plants, and can enable non-stop retrofitting of existing water plant biological treatment units.

[0049] A biofilm bypass enhancement device and system is provided. The bypass enhancement device 1 uses the biofilm characteristics on the biofilm in the biofilm packing 2 to domesticate and enrich anaerobic ammonia oxidizing bacteria 3. The bypass enhancement device 1 is connected to the main biochemical treatment system 5 through the side flow system 4, so that the cultured anaerobic ammonia oxidizing bacteria 4 can be continuously replenished to the main biochemical treatment system 5.

[0050] The bypass enhancement device 1 can control parameters such as flow rate, stirring, aeration, and added chemicals; the main biochemical treatment system 5 includes, but is not limited to, AAO, AO, multi-stage AO, SBR and other process types; the biological packing material 2 refers to the carrier that can realize the growth of anaerobic ammonia oxidation functional bacteria 3.

[0051] Sideflow system 4 refers to the reaction system that extracts a portion of the wastewater from the main biological treatment system 5 and returns it to the main biological treatment system 5. Sideflow system 4 allows the bypass enhancement device 1 to operate independently of the main biological treatment system 5, controlling parameters such as aeration and stirring.

[0052] The bypass enhancement device 1 can enrich anaerobic ammonia oxidizing bacteria 3 by controlling parameters such as influent flow rate, stirring, aeration, effluent flow rate, and the type and quantity of added chemicals.

[0053] For anaerobic ammonia-oxidizing functional bacteria 3, the following steps were performed for domestication and enrichment:

[0054] Step 1: Control the internal and external carbon sources and NO3 in the bypass enhancement device 1 - -N concentration ratio is approximately COD / NO3 --N = 6-9, reaction time approximately 50-200 minutes; NO3 - -N substances can be obtained from the main biological treatment system 5, more preferably from the effluent of its secondary sedimentation tank; COD can be controlled by adding chemicals, or it can be obtained by designing to extract it from the main biological treatment system 5, more preferably from the anoxic tank.

[0055] Step 2: Add NH4 + -N substance, stirring; NH4 + -N substances can be controlled by external reagents or obtained by designing extraction from the main biochemical treatment system 5.

[0056] Step 3: The mud-water mixture in the bypass enhancement device 1 is discharged into the main biochemical treatment system 5.

[0057] Step 4: Repeat steps 1 through 3.

[0058] The bypass enhancement device 1 and the side flow 4 can be connected with the main biological treatment system 5 by adjusting parameters such as the influent flow rate and effluent flow rate of the bypass enhancement device 1, and transport the anaerobic ammonia oxidation functional bacteria 3 into the main biological treatment system 5.

[0059] The bypass enhancement device 1 can be installed above the main process or pretreatment, advanced treatment, and other process structures, without requiring additional land. The volume of the bypass enhancement device 1 can be calculated based on the influent flow rate of the biological treatment tank and a retention time of 0.2-20 hours; more preferably, it can be calculated based on the influent flow rate of the main biological treatment system 5 and a retention time of 0.5-2 hours. The biological packing material 2 can be a hollow packing material made of materials such as plastic, polyurethane foam, volcanic rock, and activated carbon, with a specific surface area of ​​over 500 m² / m³. The volumetric filling rate of the biological packing material 2 within the external component is... 30%-90%; more preferably, the filling rate is 50%-70%; the biofilm thickness on the biological packing 2 is greater than or equal to 0.1μm, the biofilm grows continuously, and the excess biofilm falls off into the bypass enhancement device, which can be connected to the main biological treatment system 5 with the water flow; the treatment process adopted by the main biological treatment system 5 can be oxidation ditch, AO, AAO, modified AAO, UCT, multi-stage AO, SBR, CAST, etc. The present invention / utility model does not limit the process and technology adopted by the main biological treatment system 5.

[0060] Design phase:

[0061] Step 1: Based on the influent flow rate, the hydraulic retention time (HRT) of the bypass enhancement device is designed to be 1.0 h.

[0062] Step 2: The bypass enhancement device needs to be equipped with 4 inlets, which are respectively connected to the secondary sedimentation tank of the main biological treatment system, the end of the anoxic tank of the main biological treatment system, external agent 1, and external agent 2.

[0063] Domestication and enrichment operation phases:

[0064] Step 3: The main substance in the effluent from the secondary sedimentation tank of the main biological treatment system is NO3. - -N, with a concentration of approximately 6-15 mg / L, was extracted into the bypass enhancement device, occupying approximately 2 / 3 of the device's volume.

[0065] Step 4: Add external reagent 1—sodium acetate—to ensure that COD and NO3 levels in the bypass enhancement device are maintained. - -N concentration ratio COD / NO3 - -N = 6-9. For example... Figure 2 As shown.

[0066] Step 5: Fill the bypass enhancement device with 2 / 3 volume of solution and biological packing material. Stir and control the dissolved oxygen level below 0.5 mg / L for approximately 50 minutes. At the end of stirring, the COD and NO3 levels in the reactor should be low. - -N, NO2 - The concentrations of substances such as -N are approximately 0-50 mg / L, 0-6 mg / L, and 0-6 mg / L, respectively. For example... Figure 3 As shown.

[0067] Step 6: Add external reagent 2—ammonia bicarbonate, and replenish the remaining 1 / 3 volume of water in the bypass enhancement device to ensure that the NH4+ in the reactor is sufficient after addition. + The -N concentration is approximately 5-10 mg / L, which is calculated in reverse for the NH4+ in added reagent 2. + -N concentration is approximately 10-20 mg / L. For example... Figure 4 As shown.

[0068] Step 7: Stir for 1.5 hours. Figure 5 As shown.

[0069] Step 8: Drain the water from the bypass enhancement device, leaving the biological filler inside.

[0070] Step 9: Repeat steps 3-9 for 30-120 days. Anaerobic ammonia-oxidizing bacteria will have formed on the biological packing material and can be measured and identified using methods such as PCR and high-throughput sequencing. At this point, anaerobic ammonia-oxidizing bacteria have accumulated in the reactor system, thereby forming NO3-. - -N→NO2 - -N"NO2" - -N+NH4 + The reaction chain of -N→N2”.

[0071] Normal operation phase:

[0072] Step 10: The main substances at the end of the anoxic zone of the main biochemical treatment system are slowly degrading COD and NH4. + -N concentrations of approximately 30-70 mg / L and 4-20 mg / L were drawn into the bypass enhancement unit, filling approximately one-third of its volume. Meanwhile, the effluent from the secondary sedimentation tank of the biological treatment system mainly consisted of recalcitrant COD and NO3. - -N, NO3, etc. - -N concentration of approximately 6-15 mg / L is drawn into the bypass enhancement unit, filling approximately 2 / 3 of the unit's volume. If necessary, the COD concentration within the bypass enhancement unit is adjusted by adding external reagent 1.

[0073] Step 11: Stir for 50-120 minutes.

[0074] Step 12: Discharge the mud-water mixture into the aerobic tank of the main biological treatment system. Anaerobic ammonia-oxidizing bacteria will also enter the main biological treatment system with the water flow, and the following will also form in the main system:

[0075] NO3 - -N→NO2 - -N"NO2" - -N+NH4 + The reaction chain "-N→N2" increases the total nitrogen removal performance.

[0076] Step 13: Repeat steps 10-13, the process is as follows. Figure 6 As shown.

[0077] Step 14: When the abundance of anaerobic ammonia-oxidizing bacteria decreases, the enrichment process can be carried out again by returning to steps 3-9 of the "second acclimatization and enrichment stage".

[0078] Those skilled in the art can understand this embodiment as a more specific description of Embodiment 1.

[0079] Those skilled in the art will understand that, besides implementing the system and its various devices, modules, and units provided by this invention in the form of purely computer-readable program code, the same functions can be achieved entirely through logical programming of the method steps, making the system and its various devices, modules, and units of this invention function in the form of logic gates, switches, application-specific integrated circuits, programmable logic controllers, and embedded microcontrollers. Therefore, the system and its various devices, modules, and units provided by this invention can be considered as a hardware component, and the devices, modules, and units included therein for implementing various functions can also be considered as structures within the hardware component; alternatively, the devices, modules, and units for implementing various functions can be considered as both software modules implementing the method and structures within the hardware component.

[0080] Specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. A biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device, characterized in that, include: Bypass enhancement device, biological packing material, anaerobic ammonia oxidation functional bacteria, side flow system and main biochemical treatment system; The biological packing material is filled in a bypass enhancement device, in which anaerobic ammonia-oxidizing functional bacteria are domesticated. The side flow system is connected to the main biochemical treatment system, and the anaerobic ammonia-oxidizing functional bacteria enter the main biochemical treatment system through the side flow system. The bypass enhancement device enriches anaerobic ammonia-oxidizing bacteria by controlling parameters such as influent flow rate, stirring, aeration, effluent flow rate, and the type and quantity of added chemicals. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device includes the following stages in its domestication and enrichment operation: Step 1: Obtain the main substance NO3 from the effluent of the secondary sedimentation tank of the main biological treatment system. - -N; Step 2: Add the first external reagent to control COD and NO3. - -N concentration ratio; Step 3: Stir; Step 4: Add a second external reagent to control NH4. + -N concentration; Step 5: Stir; Step 6: Drain the water from the bypass enhancement device, leaving the biological filler inside the bypass enhancement device; Step 7: Repeat steps 1-6 to form "NO3" - -N→NO2 - -N”"NO2 - -N+NH4 + The reaction chain of -N→N2”; The biofilm bypass enhanced anaerobic ammonia oxidation functional bacteria device includes the following during normal operation: Step 8: The substances at the end of the anoxic zone of the main biochemical treatment system are slowly degraded COD and NH4. + -N, the mud-water mixture is drawn into the bypass enhancement device; simultaneously, the effluent from the secondary sedimentation tank of the biological treatment system contains recalcitrant COD and NO3. - -N, pump water into the bypass enhancement device; Step 9: Stir; Step 10: Discharge the mud-water mixture into the aerobic tank of the main biological treatment system. Anaerobic ammonia-oxidizing bacteria will also enter the main biological treatment system with the water flow, and the following will also form in the main system: NO2 - -N+NH4 + The reaction chain of "-N→N2" increases the total nitrogen removal performance; Step 11: Repeat steps 8-10; Step 12: When the abundance of anaerobic ammonia-oxidizing bacteria decreases, return to steps 1-7 for enrichment.

2. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 1, characterized in that, The main biochemical treatment system includes AAO, AO, multi-stage AO, and SBR process types.

3. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 1, characterized in that, The biological packing material is a carrier that promotes the growth of anaerobic ammonia-oxidizing bacteria.

4. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 1, characterized in that, The anaerobic ammonia-oxidizing functional bacteria were domesticated and enriched through the following steps: Step 1: Control the carbon source and NO3 inside and outside the bypass enhancement device - -N concentration ratio COD / NO3 - -N=6-9, stir, reaction time 50-200 minutes; Step 2: Add NH4 + -N, whose concentration is related to NO3 - The initial concentration ratio of -N in NH4 + -N / NO3 - With -N=0.8-1.5, stir and react for 50-200 minutes; Step 3: The mud-water mixture in the bypass enhancement device is discharged into the main biochemical treatment system; Step 4: Repeat steps 1-3.

5. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 4, characterized in that, In step 1: NO3 - -N substances are obtained from the effluent of the secondary sedimentation tank of the main biological treatment system; COD is obtained by extracting it from the end of the anoxic tank of the main biological treatment system.

6. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 4, characterized in that, In step 2: NH4 + -N substances were obtained by designing extraction from the main biochemical treatment system.

7. The biofilm bypass enhanced anaerobic ammonia-oxidizing bacteria device according to claim 1, characterized in that, The bypass enhancement device and side flow system are linked to the main biochemical treatment system by adjusting the influent and effluent flow parameters of the bypass enhancement device, and transport the domesticated and enriched anaerobic ammonia-oxidizing functional bacteria into the main biochemical treatment system.

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