A high-temperature conditioning agent, its preparation method and application

Abstract

This invention belongs to the field of agricultural planting technology, specifically relating to a high-temperature resistance conditioner, its preparation method, and its application. The high-temperature resistance conditioner provided by this invention is based on "traditional Chinese medicine concepts and basic principles of herbal compatibility," "the theory of chemical therapy for plant climate barriers," and "plant allelopathic and hormone regulation technology." Utilizing combinations of physiologically active substances, plant hormones, and nutrients extracted from relevant plants, it can mitigate yield and quality reductions caused by high-temperature damage, resulting in decreased grain filling rate, number of grains per ear, and grain weight in rice and corn. It can recover more than 80% of losses caused by high-temperature damage, while simultaneously increasing the rice milling rate by 15% and reducing the bleaching rate by more than 20%. In normal years without high-temperature damage, it ensures a significant yield increase of over 10% for both rice and corn.

Description

Technical Field

[0001] This invention belongs to the field of agricultural planting technology, specifically relating to a high-temperature resistant conditioning agent, its preparation method, and its application. Background Technology

[0002] Rice and corn are my country's main food crops, playing a vital role in the national economy. However, with rising global temperatures and increasingly frequent weather disasters, the production of rice and corn across the country has suffered significant losses in recent years due to adverse weather conditions such as high and low temperatures and insufficient sunlight. The yield and stability of rice and corn are crucial to food security, the development of the livestock industry, and the national economy and people's livelihoods, both domestically and globally. Therefore, improving the heat tolerance and grain-filling ability of rice and corn, and reducing the impact of natural disasters on their growth, development, yield, and quality, is extremely important and urgent.

[0003] Rice and corn often encounter various natural disasters during their growth and development. Especially when severe weather (such as high temperatures and cold damage) becomes the norm, it has the most significant impact on their growth, development, and yield. High temperatures, in particular, have a profound impact on the mid-to-late stages of rice and corn growth, development, yield, and quality. For rice, high-temperature damage in the mid-to-late stages typically refers to sustained temperatures above 35℃ during the booting stage, which can lead to incomplete development of the rice flower organs, poor pollen development, and sterility, resulting in empty grains. High temperatures during the heading and flowering stages can also affect anther dehiscence and pollen tube elongation, leading to flower sterility, increased empty grains, and ultimately, severe yield reduction and irreversible quality decline. Similarly, high temperatures and drought in the mid-to-late stages of corn growth have a significant impact on yield and quality. High-temperature damage in the mid-to-late stages of corn typically refers to corn pollen and silks encountering temperatures above the critical development temperature, preventing normal grain formation and causing severe yield reduction.

[0004] Currently, common preventative measures against high-temperature damage in the mid-to-late stages of rice and maize cultivation, both domestically and internationally, mainly involve using heat-resistant varieties, timely sowing to avoid high temperatures, and controlled fertilizer and water management. However, the number of heat-resistant varieties available for production is limited, and their yields are relatively low, thus their practical application is rare. Timely sowing to avoid high temperatures is also subject to crop rotation, limiting its effectiveness. Furthermore, high-temperature damage often occurs suddenly, and once it does, conventional cultivation management methods are largely ineffective. Therefore, improving the high-temperature resistance of rice and maize, increasing their yield and quality, and reducing losses are key issues that need to be addressed in this field. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies, resist the damage of high temperatures to crops, especially rice and corn, and improve crop yield and quality.

[0006] This invention provides a high-temperature resistant conditioning agent, which, by volume, comprises 20-70 parts of a concentrated extract of a traditional Chinese medicine composition, 15-20 parts of a first conditioning agent, and 15-20 parts of a second conditioning agent;

[0007] The herbal composition includes *Setaria viridis*, *Prunella vulgaris*, and *Coptis chinensis*; the solvent for the concentrated extract of the herbal composition is water and organophosphonic acid, with a density of 1.03–1.04 g / cm³. 3 ;

[0008] The first conditioning agent comprises GA3, NAA, 6BA, fulvic acid, and a solvent; the solvent comprises alcohol, acid, and water; the mass percentage of fulvic acid in the first conditioning agent is 0.5% to 0.75%; the mass ratio of GA3, NAA, and 6BA in the first conditioning agent is (2.2 to 2.5):1.5:1.3.

[0009] The second conditioning agent comprises, by weight parts: 1.8-2.7 parts of phytositol, 0.8-2.4 parts of threonine, 0.8-2.4 parts of serine, 0.45-1.8 parts of vitamin C, 0.45-1.8 parts of vitamin B1, 0.45-1.8 parts of vitamin B4, and 15-20 parts of water.

[0010] Preferably, the organophosphonic acid includes one or more of oxalic acid, ethylenediaminetetramethylene phosphoric acid, 2-chloroethyl phosphoric acid, and hydroxyethylidene diphosphate;

[0011] The alcohol includes ethanol; the acid includes hydrochloric acid.

[0012] Preferably, the method for preparing the extract concentrate of the traditional Chinese medicine composition includes: mixing foxtail grass, prunella vulgaris and coptis chinensis to obtain the traditional Chinese medicine composition; mixing water, organophosphonic acid and the traditional Chinese medicine composition, extracting, and concentrating to obtain the extract concentrate of the traditional Chinese medicine composition;

[0013] The extraction temperature is 50–60℃, and the time is 24–48 hours.

[0014] Preferably, the mass ratio of foxtail grass, prunella vulgaris, and coptis chinensis is 1.0:0.5:0.25;

[0015] The mass ratio of the traditional Chinese medicine composition, water, and organophosphonic acid is 2.0:12:0.5.

[0016] Preferably, the grain size of the foxtail grass, prunella vulgaris, and coptis chinensis is 60-80 mesh.

[0017] The present invention also provides a method for preparing the high-temperature conditioning agent described in the above technical solution, comprising the following steps:

[0018] GA3, NAA and alcohol in a solvent are mixed to obtain an alcohol solution;

[0019] Mix 6BA with the acid in the solvent to obtain an acid solution;

[0020] The alcohol solution, acid solution, fulvic acid, and water in the solvent are mixed to obtain the first conditioning agent;

[0021] The first conditioning agent, the second conditioning agent, and the concentrated extract of the traditional Chinese medicine composition are mixed to obtain the high-temperature resistant conditioning agent.

[0022] This invention also provides the application of the above-mentioned high-temperature conditioning agent in crop cultivation.

[0023] Preferably, the crop includes rice and / or corn.

[0024] Preferably, the high-temperature resistance conditioner is applied by diluting water during the late booting stage of the rice or 5 days before the occurrence of high-temperature damage.

[0025] The application method includes foliar spraying; the application amount is 100 mL / acre; when applying with water, the mass-to-volume ratio of water to the high-temperature resistance conditioner is 15 kg: 100 mL.

[0026] Preferably, the anti-high temperature conditioner is applied in water 5 days before the corn reaches the tasseling stage or before the occurrence of high temperature damage.

[0027] The application method includes foliar spraying; the application amount is 100 mL / acre; when applying with water, the mass-to-volume ratio of water to the high-temperature resistance conditioner is 15 kg: 100 mL.

[0028] Beneficial effects:

[0029] This invention provides a high-temperature resistant conditioning agent, comprising, by volume, 20-70 parts of a concentrated extract of a traditional Chinese medicine composition, 15-20 parts of a first conditioning agent, and 15-20 parts of a second conditioning agent; the traditional Chinese medicine composition includes *Setaria viridis*, *Prunella vulgaris*, and *Coptis chinensis*; the solvent of the concentrated extract of the traditional Chinese medicine composition is water and organophosphonic acid, with a density of 1.03-1.04 g / cm³. 3The first conditioning agent comprises GA3, NAA, 6BA, fulvic acid, and a solvent; the solvent comprises alcohol, acid, and water; the mass percentage of fulvic acid in the first conditioning agent is 0.5% to 0.75%; the mass ratio of GA3, NAA, and 6BA in the first conditioning agent is (2.2 to 2.5): 1.5: 1.3; the second conditioning agent comprises, by mass parts: 1.8 to 2.7 parts of aphrodisiac, 0.8 to 2.4 parts of threonine, 0.8 to 2.4 parts of serine, 0.45 to 1.8 parts of vitamin C, 0.45 to 1.8 parts of vitamin B1, 0.45 to 1.8 parts of vitamin B4, and 15 to 20 parts of water. This invention, based on the principles of traditional Chinese medicine and herbal compatibility, the theory of chemotherapeutic treatment of plant climate barriers, and plant allelopathic and hormone regulation technology, utilizes combinations of physiologically active substances, plant hormones, and nutrients extracted from relevant plants to mitigate yield and quality losses in rice and corn caused by high-temperature damage, resulting in decreased grain filling rate, number of grains per ear, and grain weight. It can recover over 80% of losses due to high-temperature damage, while simultaneously increasing the milled rice rate by 15% and reducing the bleaching rate of rice by over 20%. Even in years without high-temperature damage, it ensures a significant yield increase of over 10% for both rice and corn. Detailed Implementation

[0030] This invention provides a high-temperature resistant conditioning agent, which, by volume, comprises 20-70 parts of a concentrated extract of a traditional Chinese medicine composition, 15-20 parts of a first conditioning agent, and 15-20 parts of a second conditioning agent;

[0031] The herbal composition includes *Setaria viridis*, *Prunella vulgaris*, and *Coptis chinensis*; the solvent for the concentrated extract of the herbal composition is water and organophosphonic acid, with a density of 1.03–1.04 g / cm³. 3 ;

[0032] The first conditioning agent comprises GA3, NAA, 6BA, fulvic acid, and a solvent; the solvent comprises alcohol, acid, and water; the mass percentage of fulvic acid in the first conditioning agent is 0.5% to 0.75%; the mass ratio of GA3, NAA, and 6BA in the first conditioning agent is (2.2 to 2.5):1.5:1.3.

[0033] The second conditioning agent comprises, by weight parts: 1.8-2.7 parts of phytositol, 0.8-2.4 parts of threonine, 0.8-2.4 parts of serine, 0.45-1.8 parts of vitamin C, 0.45-1.8 parts of vitamin B1, 0.45-1.8 parts of vitamin B4, and 15-20 parts of water.

[0034] In this invention, the high-temperature resistant conditioning agent comprises, by volume, 20-70 parts of a concentrated extract of a traditional Chinese medicine composition, more preferably 30-60 parts, and even more preferably 40-50 parts. The concentrated extract of the traditional Chinese medicine composition provided by this invention can induce rice and corn to develop tolerance to high-temperature damage and protect cell membranes, improve the photosynthetic metabolism level of rice and corn under high-temperature conditions, and ensure the supply of nutrients and energy.

[0035] In this invention, the preferred method for preparing the extract concentrate of the traditional Chinese medicine composition includes: mixing foxtail grass, prunella vulgaris and coptis chinensis to obtain the traditional Chinese medicine composition; mixing water, organophosphonic acid and the traditional Chinese medicine composition, extracting and concentrating to obtain the extract concentrate of the traditional Chinese medicine composition.

[0036] The present invention preferably uses a mixture of foxtail grass, prunella vulgaris, and coptis chinensis to obtain a traditional Chinese medicine composition. In the present invention, the mass ratio of foxtail grass, prunella vulgaris, and coptis chinensis is preferably 1.0:0.5:0.25; the particle size of foxtail grass, prunella vulgaris, and coptis chinensis is preferably 60-80 mesh, more preferably 65-75 mesh, and even more preferably 70 mesh.

[0037] In this invention, foxtail grass is neutral in nature, bland in taste, and non-toxic. In traditional Chinese medicine, it possesses properties such as clearing heat and dampness, dispelling wind and improving eyesight, detoxifying, and killing parasites. It can treat carbuncles, sores, and conjunctivitis. Foxtail grass contains substances with strong allelopathic effects on crops, and its competitive advantage in farmland may largely stem from its allelopathic effects on crops. Using foxtail grass as a raw material and rationally utilizing its allelopathic substances can induce rice and corn to develop resistance to high-temperature damage earlier. Simultaneously, foxtail grass is rich in polysaccharides and phenolic substances. These polyphenols and other substances in foxtail grass inhibit oxidation by providing hydrogen atoms or acting as chelating agents, effectively scavenging the large amounts of hydroxyl radicals, superoxide anion radicals, and DPPH radicals produced by rice and corn due to high-temperature damage, thereby mitigating the damage. Prunella vulgaris, a plant of the genus Prunella in the Lamiaceae family, is used medicinally as its dried fruit spikes, although the whole plant is sometimes also used. In traditional Chinese medicine, Prunella vulgaris is bitter and pungent in taste, cold in nature, and enters the liver and gallbladder meridians. It can clear liver heat, improve eyesight, and reduce swelling. It also possesses various pharmacological effects, including hypoglycemic, hypotensive, antibacterial, anti-inflammatory, immunosuppressive, free radical scavenging, antioxidant, antitumor, and viral growth inhibition. Prunella vulgaris contains various chemical components, including terpenes, phenolic acids, flavonoids, sterols, coumarins, organic acids, volatile oils, and sugars. Terpenes and flavonoids are its main active substances. In modern clinical practice, it is often used to treat goiter, lymph node tuberculosis, breast hyperplasia, and as a heatstroke prevention and cooling product. Prunella vulgaris is rich in triterpenoids, with oleanolic acid and ursolic acid being the most abundant. Prunella vulgaris extract mainly exerts its antibacterial and antiviral effects by affecting the permeability of the cell wall and cell membrane of tested bacteria, inhibiting viral replication, and suppressing the pathogenesis of normal cells. Prunella vulgaris also has a bidirectional regulatory effect on the immune system. The production of secondary metabolites in plants is a result of their long-term evolutionary adaptation to harsh environments. Applying these substances to specific crops can also enhance their adaptability to adverse conditions. Furthermore, *Prunella vulgaris* polysaccharides exhibit strong scavenging abilities against hydroxyl and DPPH free radicals; *Coptis chinensis* is bitter and cold in nature, possessing the effects of clearing heat and drying dampness, purging fire and detoxifying. Berberine, the main component of *Coptis chinensis*, not only has significant antibacterial effects but also exhibits various pharmacological activities such as antiarrhythmic, hypoglycemic, antitumor, and reversal of bacterial resistance. The alkaloids, flavonoids, and terpenoids contained in *Coptis chinensis* demonstrate various pharmacological activities in medicine, including hypoglycemic, antibacterial, anti-inflammatory, antioxidant, tumor growth inhibition, and improvement of myocardial ischemia. *Coptis chinensis* contains various types of compounds, including alkaloids, lignans, coumarins, flavonoids, terpenoids, steroids, organic acids, volatile oils, and polysaccharides. Coptis chinensis also contains many non-alkaloid components: pinoresinol, (+)-5'-methoxypinoresinol, (+)-lart resin alcohol, (+)-5'-methoxylart resin alcohol, (+)-isolart resin alcohol, chlorogenic acid, ferulic acid, Z-caffeic acid stearyl ester, rhamnine, baicalin, vanillic acid, etc.Coptis chinensis contains many substances that have certain regulatory effects on crops. This invention uses foxtail grass, prunella vulgaris, and Coptis chinensis as raw materials, which contain substances with strong allelopathic effects on crops, exhibiting significant "phytoalleopathic" effects and enhancing the crops' resistance to high temperatures.

[0038] After obtaining the traditional Chinese medicine composition, the present invention preferably mixes the traditional Chinese medicine composition, water, and organophosphonic acid, and extracts the extract to obtain an extract. In the present invention, the extraction temperature is preferably 50–60°C, more preferably 52–58°C, and even more preferably 55–56°C; the extraction time is preferably 24–48 h, more preferably 48 h. During the extraction process, the mixture is preferably stirred for 5 min every 2 h. The mass ratio of the traditional Chinese medicine composition, water, and organophosphonic acid is preferably 2.0:12:0.5. The organophosphonic acid preferably includes one or more of ethylenediphosphate, ethylenediaminetetramethylene phosphoric acid, 2-chloroethylphosphonic acid, and hydroxyethylidene diphosphate, more preferably ethylenediphosphate. The present invention uses water and organophosphonic acid as extraction agents. The extract obtained by extracting organophosphonic acid in combination with water has a significant "phytoallemetic" effect on crop heat resistance, and by limiting the extraction conditions, the heat resistance effect of the active ingredients can be further improved, resulting in a more effective resistance to heat damage compared to general extraction methods.

[0039] After obtaining the extract, the present invention preferably filters the extract, collects the filtrate, allows it to stand, and collects the supernatant. In the present invention, the filtration method is preferably pressure filtration. The standing time is preferably 12 hours to allow the filtrate to settle naturally.

[0040] After obtaining the supernatant, the present invention preferably concentrates the supernatant to obtain the concentrated extract of the traditional Chinese medicine composition. The concentration method of the present invention preferably includes negative pressure distillation. The present invention does not have strict requirements on the concentration parameters, aiming to obtain a concentrated extract of the traditional Chinese medicine composition with a density of 1.03–1.04 g / cm³. 3 That is, the pH of the concentrated extract of the traditional Chinese medicine composition is preferably adjusted to 1-2.

[0041] Based on the volume fraction of the concentrated extract of the traditional Chinese medicine composition, the high-temperature conditioning agent of the present invention further includes 15-20 parts of a first conditioning agent, preferably 16-18 parts. The first conditioning agent of the present invention includes GA3, NAA, 6BA, fulvic acid, and a solvent; the solvent includes alcohol, acid, and water; the mass percentage of fulvic acid in the first conditioning agent is 0.5%-0.75%; the mass ratio of GA3, NAA, and 6BA in the first conditioning agent is (2.2-2.5):1.5:1.3, preferably 2.2:1.5:1.3.

[0042] In this invention, the preferred mass percentages of GA3, NAA, and 6BA in the first conditioner are 6.6%, 4.5%, and 3.9%, respectively. The alcohol in this invention is preferably ethanol; the acid is preferably hydrochloric acid. The ratios of GA3 / ABA, IAA(NAA) / ABA, and 6-BA / ABA in leaves determine the degree of stomatal opening under high temperature (drought). GA3 has a significant yield-increasing effect, 6-BA can improve cell metabolism, slow down cell physiological aging, enhance photosynthetic efficiency, promote root vitality, and maintain a reasonable IAA(NAA) level, which can promote physiological metabolism and growth and development. The combined use of GA3, NAA, and 6BA in this invention, by limiting the mass ratios of GA3, NAA, and 6BA, can better regulate the ratios of GA3 / ABA, IAA(NAA) / ABA, and 6-BA / ABA, thereby regulating the root and stem vitality, stomatal conductance, and transpiration rate of rice and corn, enhancing the canopy heat dissipation effect of rice and corn, and thus resisting high temperatures; the fulvic acid can improve stability. This invention uses GA3, NAA, 6BA, and fulvic acid as the active ingredients of the first conditioner. It can significantly improve the ratio of cytokinin-like hormones, GA-like hormones, and NAA-like auxin-like hormones to ABA hormones in rice and maize when subjected to high-temperature damage. This promotes stomatal opening in leaves, increases transpiration efficiency, and prevents heat damage. Simultaneously, it promotes CO2 flow, thereby improving photosynthetic efficiency and ensuring energy supply, providing a material basis for high yield. It activates the activity of enzymes in the plant, catalyzing cells to accelerate the absorption of water and nutrients, regulating photosynthesis and transpiration, promoting new root growth, increasing root development, and improving vitality. This invention uses alcohol, acid, and water as solvents, which reduces costs.

[0043] Based on the volume fraction of the concentrated extract of the traditional Chinese medicine composition, the high-temperature conditioning agent of the present invention further includes 15-20 parts of a second conditioning agent. By mass fraction, the second conditioning agent of the present invention comprises: 1.8-2.7 parts of a tonic, 0.8-2.4 parts of threonine, 0.8-2.4 parts of serine, 0.45-1.8 parts of vitamin C, 0.45-1.8 parts of vitamin B1, 0.45-1.8 parts of vitamin B4, and 15-20 parts of water. This invention preferably uses 90% phytoalexin to provide phytoalexin for the anti-heat conditioning agent of this invention, and the mass fraction of the 90% phytoalexin is preferably 2 to 4 parts; this invention preferably uses 80% threonine to provide threonine for the anti-heat conditioning agent of this invention, and the mass fraction of the 80% threonine is preferably 1 to 3 parts based on the mass fraction of the 90% phytoalexin; this invention preferably uses 80% serine to provide serine for the anti-heat conditioning agent of this invention, and the mass fraction of the 80% serine is preferably 1 to 3 parts based on the mass fraction of the 90% phytoalexin; this invention preferably uses 90% The present invention provides VC for its heat-resistant conditioning agent, with the preferred mass percentage of the 90% VC being 0.5 to 2 parts based on the mass percentage of the 90% VC. The present invention also preferably uses 90% VB1 to provide VB1 for its heat-resistant conditioning agent, with the preferred mass percentage of the 90% VB1 being 0.5 to 2 parts based on the mass percentage of the 90% VB1. Furthermore, the present invention preferably uses 90% VB4 to provide VB4 for its heat-resistant conditioning agent, with the preferred mass percentage of the 90% VB4 being 0.5 to 2 parts based on the mass percentage of the 90% VB4. The present invention selects threonine, serine, and water-soluble vitamins that have significant effects on high-temperature heat damage. Vitamin C, Vitamin B1, and Vitamin B4 actively participate in cell repair in rice and maize under high-temperature stress. They also stimulate the activity of the secondary metabolite system in rice and maize, replenishing the loss of physiologically active substances (such as vitamins) caused by high temperatures. This accelerates the repair of high-temperature damage, enhances photosynthesis and the conversion of photosynthetic products, ensures the supply of organic matter needed for pollen (filaments) and grain filling under high temperatures, and increases the seed setting rate, thousand-grain weight, and yield of rice and maize. Threonine and serine are important osmotic regulators in rice and maize under high-temperature stress, improving their adaptation to high-temperature stress, promoting amino acid balance, protecting cell membranes, and reducing the decomposition of soluble proteins caused by high temperatures. The growth promoter (pix) described in this invention can increase the amount of chloroplasts and mitochondria, ensuring sufficient melatonin synthesis sites and thus increasing melatonin levels. Melatonin can significantly enhance cellular resistance to heat damage. Within the scope specified in this invention, Pix can increase the chlorophyll content in the functional leaves of rice and maize, enhance photosynthesis and the conversion capacity of photosynthetic products, ensure the supply of organic matter required for grain filling under high temperature, and increase the seed setting rate, thousand-grain weight, and yield.The second heat-resistant conditioning agent of the present invention contains amino acids and vitamins, which can significantly reduce the effects of high temperature.

[0044] In this invention, the high-temperature resistant conditioning agent preferably further includes water; the total volume fraction of the water, the concentrated extract of the traditional Chinese medicine composition, the first conditioning agent, and the second conditioning agent is preferably 100 parts.

[0045] The present invention preferably also provides a method for preparing the high-temperature conditioning agent described in the above technical solution, comprising the following steps:

[0046] The GA3, NAA, and alcohol in the solvent are mixed to obtain an alcohol solution;

[0047] The 6BA and the acid in the solvent are mixed to obtain an acid solution;

[0048] The alcohol solution, acid solution, fulvic acid, and water in the solvent are mixed to obtain the first conditioning agent;

[0049] The first conditioning agent, the second conditioning agent, and the concentrated extract of the traditional Chinese medicine composition are mixed to obtain the high-temperature resistant conditioning agent.

[0050] In this invention, GA3, NAA, and an alcohol in the solvent are mixed to obtain an alcohol solution. Preferably, the mass ratio of GA3, NAA, and alcohol in the alcohol solution is 6.6:4.5:50. More preferably, GA3 is first mixed with an alcohol to obtain an alcohol solution of GA3, and then NAA is dissolved to obtain an alcohol solution of GA3 and NAA. The alcohol used in this invention is preferably anhydrous ethanol.

[0051] This invention involves mixing the 6BA with an acid in the solvent to obtain an acid solution. In this invention, the preferred mass ratio of the 6BA to the acid is 3.9:20; the preferred acid is hydrochloric acid; and the preferred hydrochloric acid is analytical grade.

[0052] After obtaining the alcohol solution and acid solution, the present invention mixes the alcohol solution, acid solution, fulvic acid, and water from the solvent to obtain the first conditioning agent. The mass ratio of fulvic acid to GA3 in the present invention is preferably 0.5–0.75:6.6, more preferably 0.5:6.6. The total volume of the first conditioning agent in the present invention is preferably X, where X (mL) = mass of GA3 / 6.6 g * 100 mL.

[0053] After obtaining the first conditioning agent, the present invention preferably mixes the first conditioning agent, the second conditioning agent, and the concentrated extract of the traditional Chinese medicine composition to obtain the high-temperature resistant conditioning agent. The method for extracting the concentrated extract of the traditional Chinese medicine composition described in this invention has been described above and will not be repeated here. The present invention does not have strict requirements on the preparation method of the second conditioning agent; simply mixing the components of the second conditioning agent is sufficient.

[0054] In this invention, the preferred method for mixing the first conditioning agent, the second conditioning agent, and the concentrated extract of the traditional Chinese medicine composition includes: mixing the first conditioning agent and the second conditioning agent to obtain a mixture, and then mixing the mixture with the concentrated extract of the traditional Chinese medicine composition. This invention's specific mixing order further improves product stability.

[0055] This invention also provides the application of the high-temperature resistant conditioner described in the above-mentioned technical solution in crop cultivation. In this invention, the crop preferably includes rice and / or corn.

[0056] In this invention, when applied to rice cultivation, the heat-resistant conditioner is preferably applied by diluting water 5 days (inclusive) before the occurrence of high-temperature damage to rice or during the late booting stage of rice. The application method preferably includes foliar spraying. The preferred application amount of the heat-resistant conditioner is 100 mL / mu. When diluting water for application, the preferred mass-volume ratio of water to heat-resistant conditioner is 15 kg: 100 mL.

[0057] In this invention, when applied to corn planting, it is preferred that the anti-heat-temperature conditioner be diluted with water and applied 5 days (inclusive) before the corn's large trumpet stage or before the occurrence of high-temperature damage; the application method is preferably foliar spraying; the application amount of the anti-heat-temperature conditioner is preferably 100 mL / mu; when diluted with water, the mass-volume ratio of water to the anti-heat-temperature conditioner is preferably 15 kg: 100 mL.

[0058] The high-temperature resistance conditioner provided by this invention, when sprayed on the leaves of rice and corn 5 days (inclusive) before high-temperature damage, can enhance the adaptability and resistance of rice and corn plants to high-temperature adversity by stimulating the activity of enzymes in the plants. It promotes stomatal opening, improves transpiration and heat dissipation efficiency, reduces heat damage, increases root vitality, enhances water transport efficiency, and further promotes transpiration and heat dissipation. It also protects the integrity of cell membrane structure, improves intercellular material exchange, ensures vigorous physiological and biochemical metabolism, and promotes growth and development. The combined induction of heat shock proteins by plant allelopathic active substances significantly enhances the high-temperature resistance of pollen (filaments). This can prevent or significantly reduce yield losses caused by high temperatures affecting seed setting rate and thousand-grain weight, thereby increasing seed setting rate and the number of filled grains per ear, promoting photosynthesis, and accelerating the conversion and accumulation of photosynthetic substances to improve yield and quality. It also has a very significant yield-increasing effect when no high-temperature weather occurs, truly achieving an organic combination of disaster prevention and yield protection during high-temperature disasters and increased yield, quality, and efficiency during the absence of high-temperature disasters.

[0059] To further illustrate the present invention, the following detailed description, in conjunction with embodiments, provides a high-temperature conditioning agent, its preparation method, and its application, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0060] Example 1

[0061] A high-temperature conditioning agent

[0062] 1. Proportions of each component

[0063] The total volume is 100ml (parts) consisting of 45ml (parts by volume) of the concentrated extract of the traditional Chinese medicine composition, 18ml (parts by volume) of the first conditioning agent, 20ml (parts by volume) of the second conditioning agent, and the remainder water.

[0064] 2. Preparation method of high-temperature conditioning agent

[0065] The preparation method of 100mL of high-temperature resistant conditioning agent is used as an example for illustration. In specific applications, the scale can be increased proportionally according to actual needs:

[0066] (1) Preparation of concentrated extract of traditional Chinese medicine composition

[0067] The dried medicinal plants *Setaria viridis*, *Prunella vulgaris*, and *Coptis chinensis* were pulverized into 60-80 mesh and mixed at a mass ratio of *Setaria viridis*: *Prunella vulgaris*: *Coptis chinensis* = 1.0:0.5:0.25. The resulting dry powder was then mixed with water and ethylene diphosphonic acid at a mass ratio of 2.0:12:0.5 and extracted at 50-60℃ for 24 hours, stirring for 5 minutes every 2 hours. After the extraction time was up, the mixture was filtered through a filter cloth. The filtrate was allowed to stand for 12 hours to allow natural sedimentation, and the supernatant was then concentrated by negative pressure distillation to a specific gravity of 1.03-1.04 g / cm³. 3 To ensure the concentration of the extract, the pH of the extract is adjusted to be controlled between 1 and 2, thus obtaining a concentrated extract of the traditional Chinese medicine composition.

[0068] (2) Preparation of the first conditioning agent

[0069] First, dissolve 6.6g of GA3 in 50g of anhydrous ethanol, then dissolve 4.5g of NAA in the same solution, and dissolve 3.9g of 6BA in 20g of hydrochloric acid (analytical grade). Then combine the two solutions and add 0.5g of fulvic acid. Finally, dilute to 100mL with deionized water. The mass ratio of GA3:NAA:6BA is 2.2:1.5:1.3, thus obtaining the first conditioning agent.

[0070] (3) Preparation of the second conditioning agent

[0071] Take 20 mL of deionized water, and then dissolve 4 g of 90% growth promoter, 3 g of 80% threonine, 3 g of 80% serine, 2 g of 90% vitamin C, 2 g of 90% vitamin B1, and 2 g of 90% vitamin B4 in the deionized water to obtain the second conditioning agent;

[0072] (4) Preparation of high-temperature conditioning agent

[0073] Take 18 mL of the first conditioning agent obtained in step (2) and slowly pour it into the second conditioning agent obtained in step (3), stirring while pouring, to obtain the first premixed solution;

[0074] Take 45 mL of the extract concentrate of the Chinese herbal composition obtained in step (1), slowly pour it into the first premixed solution while stirring, and obtain the second premixed solution. Make up to 100 mL with deionized water to obtain the high temperature resistant conditioning agent.

[0075] Examples 2-6

[0076] The high-temperature resistant conditioning agent was prepared according to the method of Example 1. In Examples 2-6, compared with Example 1, the dosage of the concentrated extract of the traditional Chinese medicine composition and the first conditioning agent were different, the composition of the second conditioning agent was different, and other conditions were the same. In Examples 7-11, compared with Example 1, the dosage of the concentrated extract of the traditional Chinese medicine composition and the first conditioning agent were different, the composition of the second conditioning agent was the same, and other conditions were the same. See Table 1 for details.

[0077] Compared with Example 2, Examples 12, Comparative Examples 1-6 differ only in the ratio of the first conditioner, as detailed in Table 2;

[0078] Compared with Example 2, Examples 13-14, Comparative Examples 7-8, and Example 2 differed only in the concentration of the first conditioner, fulvic acid, as detailed in Table 3.

[0079] Compared with Example 2, Examples 15-16, Comparative Examples 9-11, and Example 2 differ only in the ratio of the first conditioner, as detailed in Table 4;

[0080] Compared with Comparative Examples 12-17 and Example 2, only the mass ratios of foxtail grass, prunella vulgaris, and coptis chinensis are different, as shown in Table 5.

[0081] Compared with Example 2, Examples 17-23 differ only in the type of organophosphonic acid or the extraction time when preparing the extract concentrate of the traditional Chinese medicine composition, as detailed in Table 6.

[0082] Compared with Example 2, Examples 24-29 differ only in the amount of the concentrated extract of the traditional Chinese medicine composition and the first conditioning agent, as detailed in Table 7.

[0083] Table 1. High-Temperature Conditioning Agents of Examples 1-11 and Comparative Example 1

[0084]

[0085] Table 2. High-Temperature Conditioning Agents of Examples 2, 12 and Comparative Examples 1-6

[0086]

[0087] Table 3. High-Temperature Conditioning Agents for Examples 2, 13-14 and Comparative Examples 9-10

[0088] First conditioning agent (mL) 20 20 20 20 20 Fulvic acid (g / 100mL first conditioning agent) 0.5 0.75 0.5 0.25 0.00

[0089] Table 4. High-Temperature Conditioning Agents for Examples 15-16 and Comparative Examples 9-11

[0090]

[0091]

[0092] Table 5. High-Temperature Conditioning Agents for Examples 2 and Comparative Examples 14-20

[0093] Comparative Example 12 0：0.5：0.25 Comparative Example 13 0.5：1.0：0.25 Example 2 1.0：0.5：0.25 Comparative Example 14 2.0：0.5：0.25 Comparative Example 15 2.0：1.0：2.5 Comparative Example 16 1.0：0.0：0.5 Comparative Example 17 1.0：1.0：0.0

[0094] Table 6. High-Temperature Conditioning Agents from Examples 2 and 17-24

[0095]

[0096] Table 7. High-Temperature Conditioning Agents in Examples 2 and 24-28

[0097] Example 24 20 20 Example 25 20 30 Example 26 20 40 Example 2 20 50 Example 27 20 60 Example 28 20 70

[0098] Comparative Example 18

[0099] Same as Example 2, except that the second conditioning agent does not contain growth promoters.

[0100] Comparative Example 19

[0101] Same as Example 2, except that the second conditioner does not contain VC, VB1 and VB4.

[0102] Comparative Example 20

[0103] Same as Example 2, except that the second conditioner does not contain threonine and serine.

[0104] Comparative Example 21

[0105] Same as Example 2, except that the second conditioning agent contains only a growth promoter and the remainder is deionized water.

[0106] Comparative Example 22

[0107] Same as Example 2, except that the second conditioner contains only VC, VB1, VB4 and the remainder deionized water.

[0108] Comparative Example 23

[0109] Same as Example 2, except that the second conditioner contains only threonine, serine and the remainder deionized water.

[0110] Comparative Example 24

[0111] Same as Example 2, except that the high-temperature conditioning agent does not contain the concentrated extract of the traditional Chinese medicine composition.

[0112] Comparative Example 25

[0113] Same as Example 2, except that the high-temperature conditioning agent does not contain the first conditioning agent.

[0114] Comparative Example 26

[0115] Same as Example 2, except that the high-temperature conditioning agent does not contain a second conditioning agent.

[0116] Comparative Example 27

[0117] Same as Example 2, except that the high-temperature conditioning agent does not contain the first conditioning agent and the second conditioning agent.

[0118] Comparative Example 28

[0119] Same as Example 2, except that the high-temperature conditioning agent does not contain the concentrated extract of the traditional Chinese medicine composition and the second conditioning agent.

[0120] Application Example 1

[0121] 1. Selection of test site and test varieties:

[0122] In 2022, Wu Moutian, a large-scale grain grower in Lianshui, conducted an experiment in his contracted land in Xuexing Village, Zhuma Town, Lianshui County, Jiangsu Province. The soil fertility was moderate to high, and the previous crop was wheat.

[0123] The rice varieties tested were indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308). Rice was sown on May 15, 2022, using plastic hard disk substrate for seedling raising, with 80 grams of seeds per tray. Transplanting took place on June 20. Row and plant spacing was 3cm × 13.3cm for indica rice (Q Liangyou 879), with 16,700 hills per mu (approximately 6,400 hectares) and a basic seedling density of 65,000 seedlings per mu (approximately 4,300 hectares); for japonica rice (Nanjing 9308), with 30cm × 10cm spacing, with 22,000 hills per mu (approximately 1,400 hectares) and a basic seedling density of 88,000 seedlings per mu (approximately 5,400 hectares). To ensure the uniformity of population size across treatments, the number of panicles in each treatment plot was strictly controlled: 180,000 panicles per mu for indica rice (Q Liangyou 879) and 220,000 panicles per mu for japonica rice (Nanjing 9308).

[0124] The above test sites and test varieties were randomly divided into 3 areas for field trials of Application Example 1, Application Example 2 and Application Example 3, with each application example being conducted simultaneously.

[0125] 2. Experimental grouping and treatment methods

[0126] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator treatment and control 1 (CK1) treatment; the high temperature treatment was randomly divided into regulator treatment and control 2 (CK2) treatment.

[0127] Room temperature treatment - regulator treatment: In the late booting stage of rice (before heading), take the high temperature resistance regulator obtained in Example 1 at a dosage of 100 mL / mu, dilute 100 mL of the high temperature resistance regulator with 15 kg of water, and spray it evenly on the rice leaves. After spraying, cultivate as usual.

[0128] Room temperature treatment - CK1 treatment: In the late booting stage of rice (before heading), spray water on the rice leaves. The amount of water sprayed is the same as that of the room temperature treatment - regulator treatment. After spraying, conventional cultivation is carried out.

[0129] High temperature treatment - regulator treatment: In the late booting stage of rice (before heading), take the high temperature resistance regulator obtained in Example 1 at a dosage of 100 mL / mu, dilute 100 mL of the high temperature resistance regulator with 15 kg of water, and spray it evenly on the rice leaves. On the fifth day after spraying the regulator, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to lower the temperature. At night, allow the temperature to drop naturally. Continue the treatment until the rice enters the grain filling stage.

[0130] High-temperature treatment - CK2 treatment: In the late booting stage of rice (before heading), spray water on the rice leaves. The amount of water sprayed is the same as that of the normal temperature treatment - conditioner treatment. On the fifth day after spraying the conditioner, carry out high-temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to lower the temperature. At night, allow the temperature to drop naturally. Continue the treatment until the rice enters the grain filling stage.

[0131] All the above treatment groups were implemented with three replicates, and the plot area for each replicate was 5.0 × 2.0 = 10 m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0132] 3. Evaluation Indicators: Physiological and biochemical indicators include chlorophyll, SOD, MDA, leaf transpiration rate (Tr), stomatal conductance (Gs), net photosynthetic rate (Pn), and root damage flow in the later stage; yield composition indicators include ear number / mu, number of filled grains / ear, thousand-grain weight, seed setting rate, yield, milled rice rate, bleaching rate, and heat damage recovery rate. Among them, physiological and biochemical indicators were sampled and tested 30 days after conditioner treatment; yield composition was examined before harvest, and the test results are shown in Tables 8 and 9.

[0133] Recovery rate of heat damage (%) = (Production of regulator at high temperature - Production of CK2) / (Production of CK1 - Production of CK2) * 100

[0134] Table 8. Results of Physiological and Biochemical Tests

[0135]

[0136]

[0137] Table 9 Results of Production Structure Detection

[0138]

[0139] As shown in Tables 8-9, under high-temperature treatment, after spraying the high-temperature resistance conditioner of Example 1, compared with the control CK2, the following effects were observed in indica rice varieties (Q Liangyou 879) and japonica rice varieties (Nanjing 9308): the late-stage root and stem sap flow increased by 89.89% and 87.38%, respectively, with an average of 88.64%; the chlorophyll content of the flag leaf increased by 19.26% and 17.8%, respectively, with an average of 18.53%; the SOD activity increased by 42.85% and 44.02%, respectively, with an average of 43.44%; the MDA content decreased by 25.4% and 23.57%, respectively, with an average of 24.49%; the leaf transpiration rate Tr increased by 62.50% and 61.05%, respectively, with an average of 61.78%; and the stomatal conductance Gs increased by 63.33%, respectively. The yield increased by 62.96%, with an average of 63.15%; the net photosynthetic rate (Pn) increased by 29.10% and 32.84%, with an average of 30.97%; the thousand-grain weight increased by 3.04% and 5.88%, with an average of 4.46%; the seed setting rate increased by 30.66% and 18.61%, with an average of 24.64%; the number of filled grains per ear increased by 31.56% and 19.13%, with an average of 25.35%; the yield increased by 36.6% and 30.27%, with an average of 33.44%; the heat damage loss recovery rate increased by 83.56% and 84.71%, with an average of 84.14%; the milling rate increased by 20.83% and 6.56%, with an average of 13.70%; and the bleaching rate decreased by 22.99% and 23.19%, with an average of 23.09%.

[0140] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 1, compared with the control CK1, the following effects were observed in indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308): root and stem sap flow increased by 54.3% and 54.7% respectively, with an average of 54.5%; chlorophyll content in flag leaves increased by 12.77% and 16.92% respectively, with an average of 14.85%; SOD activity increased by 21.73% and 23.35% respectively, with an average of 22.54%; MDA content decreased by 30.34% and 30.46% respectively, with an average of 30.4%; and leaf transpiration rate (Tr) increased by 26.43% and 20.94% respectively, with an average of 23.69%. Stomatal conductance (Gs) increased by 21.05% and 24.24%, respectively, with an average of 22.65%; net photosynthetic rate (Pn) increased by 13.63% and 14.12%, respectively, with an average of 13.88%; thousand-grain weight increased by 0.36% and 3.04%, respectively, with an average of 1.7%; seed setting rate increased by 8.49% and 7.52%, respectively, with an average of 8.01%; number of grains per ear increased by 10.60% and 9.98%, respectively, with an average of 10.29%; yield increased by 12.1% and 10.35%, respectively, with an average of 11.23%; milling rate increased by 3.99% and 1.50%, respectively, with an average of 2.75%; and bleaching rate decreased by 7.44% and 2.12%, respectively, with an average of 4.78%.

[0141] The high-temperature resistance conditioner provided in Example 1 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of rice under high-temperature conditions.

[0142] Application Example 2

[0143] Same as Application Example 1, except that the high-temperature conditioner in Example 1 of Application Example 1 was replaced with the high-temperature conditioner in Example 2. The test results are shown in Tables 10-11.

[0144] Table 10 Results of Physiological and Biochemical Tests

[0145]

[0146] Table 11 Results of Production Structure Detection

[0147]

[0148]

[0149] As shown in Tables 10-11, under high-temperature treatment, compared with the control CK2, spraying the high-temperature resistance conditioner of Example 2 increased the root and stem sap flow by 98.41% and 97.11% respectively (average 97.76%) in indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308) in the later stages; increased the chlorophyll content of flag leaves by 20.52% and 19.35% respectively (average 19.94%); increased SOD activity by 45.15% and 44.87% respectively (average 45.01%); decreased MDA content by 20.8% and 23.29% respectively (average 22.05%); increased leaf transpiration rate Tr by 53.96% and 67.64% respectively (average 60.80%); and increased stomatal conductance Gs by 71.43%. The average increase was 66.64%; the net photosynthetic rate (Pn) increased by 30.73% and 41.54%, respectively, with an average of 36.14%; the thousand-grain weight increased by 4.65% and 4.47%, respectively, with an average of 4.56%; the seed setting rate increased by 29.92% and 19.97%, respectively, with an average of 24.95%; the number of grains per ear increased by 30.73% and 18.26%, respectively, with an average of 24.50%; the yield increased by 35.35% and 23.77%, respectively, with an average of 29.56%; the heat damage loss recovery rate increased by 87.29% and 76.67%, respectively, with an average of 81.98%; the milling rate increased by 25.34% and 7.75%, respectively, with an average of 16.55%; and the bleaching rate decreased by 19.89% and 20.36%, respectively, with an average of 20.13%.

[0150] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 2, compared with the control CK1, the following effects were observed in indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308): root and stem sap flow increased by 59.48% and 65.18% respectively, with an average of 62.33%; chlorophyll content in flag leaves increased by 16.07% and 15.93% respectively, with an average of 16.0%; SOD activity increased by 22.59% and 29.90% respectively, with an average of 26.25%; MDA content decreased by 31.20% and 29.86% respectively, with an average of 30.53%; and leaf transpiration rate (Tr) increased by 21.85% and 16.08% respectively, with an average of 18.97%. Stomatal conductance (Gs) increased by 21.62% and 30.77%, respectively, with an average of 26.20%; net photosynthetic rate (Pn) increased by 12.52% and 16.32%, respectively, with an average of 14.42%; thousand-grain weight increased by 2.22% and 1.89%, respectively, with an average of 2.06%; seed setting rate increased by 7.63% and 3.67%, respectively, with an average of 5.65%; number of filled grains per ear increased by 11.26% and 9.77%, respectively, with an average of 10.52%; yield increased by 12.07% and 1.26%, respectively, with an average of 11.67%; milling rate increased by 3.24% and 2.21%, respectively, with an average of 2.73%; and bleaching rate decreased by 10.61% and 7.53%, respectively, with an average of 9.07%.

[0151] The high-temperature resistance conditioner provided in Example 2 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of rice under high-temperature conditions.

[0152] Application Example 3

[0153] Same as Application Example 1, except that the high-temperature conditioner in Example 1 of Application Example 1 was replaced with the high-temperature conditioner in Example 3. The test results are shown in Tables 12-13.

[0154] Table 12 Results of Physiological and Biochemical Tests

[0155]

[0156] Table 13 Results of Production Structure Detection

[0157]

[0158] As shown in Tables 12 and 13, under high-temperature treatment, after spraying the high-temperature resistance conditioner of Example 3, compared with the control CK2, the following effects were observed in indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308): the root and stem sap flow increased by 89.07% and 92.30% respectively, with an average of 90.69%; the chlorophyll content of the flag leaf increased by 20.10% and 21.88% respectively, with an average of 20.99%; the SOD activity increased by 40.37% and 45.79% respectively, with an average of 43.08%; the MDA content decreased by 24.62% and 23.02% respectively, with an average of 23.82%; the leaf transpiration rate Tr increased by 55.68% and 66.20% respectively, with an average of 60.94%; and the stomatal conductance Gs increased by 74%. The percentages of rice yield and grain size increased by 72.0% and 72.0%, respectively, with an average of 73.04%; the net photosynthetic rate (Pn) increased by 28.99% and 37.53%, respectively, with an average of 33.26%; the thousand-grain weight increased by 6.23% and 0.39%, respectively, with an average of 3.31%; the seed setting rate increased by 31.13% and 20.03%, respectively, with an average of 25.58%; the number of grains per ear increased by 30.34% and 20.04%, respectively, with an average of 25.19%; the yield increased by 29.20% and 28.40%, respectively, with an average of 28.80%; the heat damage loss recovery rate increased by 82.8% and 80.31%, respectively, with an average of 81.56%; the milling rate increased by 23.8% and 6.89%, respectively, with an average of 15.35%; and the bleaching rate decreased by 20.89% and 21.29%, respectively, with an average of 21.09%.

[0159] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 3, compared with the control CK1, the following results were observed in indica rice (Q Liangyou 879) and japonica rice (Nanjing 9308): root and stem sap flow increased by 46.93% and 48.37% respectively, with an average of 47.65%; chlorophyll content in flag leaves increased by 10.17% and 16.74% respectively, with an average of 13.46%; SOD activity increased by 18.88% and 28.53% respectively, with an average of 23.71%; MDA content decreased by 35.76% and 33.29% respectively, with an average of 34.53%; and leaf transpiration rate (Tr) increased by 24.53% and 22.14% respectively, with an average of 23.34%. The percentages of grain yield increased; stomatal conductance (Gs) increased by 22.82% and 38.13%, respectively, with an average of 30.48%; net photosynthetic rate (Pn) increased by 13.72% and 14.60%, respectively, with an average of 14.16%; thousand-grain weight increased by -2.16% and 4.60%, respectively, with an average of 1.22%; grain setting rate increased by 7.39% and 7.13%, respectively, with an average of 7.26%; grains per ear increased by 11.15% and 7.08%, respectively, with an average of 9.12%; yield increased by 13.4% and 12.94%, respectively, with an average of 13.17%; milling rate increased by 3.69% and 2.44%, respectively, with an average of 3.07%; and bleaching rate decreased by 8.43% and 6.22%, respectively, with an average of 7.33%.

[0160] The high-temperature resistance conditioner provided in Example 3 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of rice under high-temperature conditions.

[0161] Application Example 4

[0162] 1. Selection of test site and test varieties:

[0163] In 2022, a large-scale grain grower, Mr. Ding, conducted an experiment on his dryland contracted land in Beiwuji Village, Huaiyin District (a dryland grain crop demonstration base in Huaiyin District, Huai'an City, Jiangsu Province). The soil was sandy loam with medium to low fertility, and the previous crop was wheat.

[0164] The tested maize varieties were Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant). The maize was sown on June 20, 2022, at a planting density of 5,000 plants per mu.

[0165] The above test sites and test varieties were randomly divided into 3 areas for field trials of Application Example 4, Application Example 5 and Application Example 6, with each application example being conducted simultaneously.

[0166] 2. Experimental grouping and treatment methods

[0167] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator treatment and control 1 (CK1) treatment; the high temperature treatment was randomly divided into regulator treatment and control 2 (CK2) treatment.

[0168] Room temperature treatment - regulator treatment: At the corn tasseling stage, take the high temperature resistance regulator obtained in Example 4 at a dosage of 100 mL / mu, dilute 100 mL of high temperature resistance regulator with 15 kg of water, and spray it evenly on the corn leaves. After spraying, cultivate as usual.

[0169] Normal temperature treatment - CK1 treatment: Spray water on corn leaves at the corn tasseling stage, with the same amount of water as the normal temperature treatment - regulator treatment, and then cultivate as usual after spraying.

[0170] High temperature treatment - regulator treatment: At the corn tasseling stage, take the high temperature resistance regulator obtained in Example 4 at a dosage of 100 mL / mu, dilute 100 mL of the high temperature resistance regulator with 15 kg of water, and spray it evenly on the rice leaves. On the fifth day after spraying the regulator, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. At night, allow the temperature to drop naturally. Continue the treatment until the corn flowers and pollinates.

[0171] High temperature treatment - CK2 treatment: Spray water on corn leaves during the corn tasseling stage. The amount of water sprayed is the same as that of the normal temperature treatment - conditioner treatment. On the fifth day after spraying the conditioner, carry out high temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue this treatment until the corn flowers and pollinates.

[0172] All the above treatment groups were implemented with three replicates, and the plot area for each replicate was 48m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0173] 3. Evaluation Indicators: Physiological and biochemical indicators include chlorophyll, SOD, MDA, leaf transpiration rate (Tr), stomatal conductance (Gs), net photosynthetic rate (Pn), and stem sap flow; yield composition indicators include row number / ear, grain number / row, filled grain number / ear, thousand-grain weight, barren tip length, yield, and heat damage recovery rate. Among these, physiological and biochemical indicators were sampled and tested 30 days after conditioner treatment; yield composition indicators were examined before harvest, and the test results are shown in Tables 14 and 15.

[0174] Recovery rate of heat damage (%) = (Production of regulator at high temperature - Production of CK2) / (Production of CK1 - Production of CK2) * 100

[0175] Table 14 Results of Physiological and Biochemical Tests

[0176]

[0177] Table 15 Results of Production Structure Detection

[0178]

[0179]

[0180] As shown in Tables 14-15, under high-temperature treatment, after spraying the high-temperature resistance conditioner of Example 4, compared with the control CK2, the following effects were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): the root and stem sap flow increased by 55.21% and 64.0% respectively, with an average of 59.61%; the chlorophyll content of ear leaves increased by 2.63% and 26.14% respectively, with an average of 14.39%; SOD activity increased by 49.53% and 54.87% respectively, with an average of 52.2%; MDA content decreased by 25.65% and 29.87% respectively, with an average of 27.76%; and leaf transpiration rate (Tr) increased by 40.91% and 53.66% respectively, with an average of 47.29%. Pore ​​conductance (Gs) increased by 130.0% and 156.25%, respectively, with an average of 143.13%; net photosynthetic rate (Pn) increased by 35.98% and 16.2%, respectively, with an average of 26.09%; thousand-grain weight increased by 0.29% and 0.28%, respectively, with an average of 0.285%; grain number per row increased by 20.08% and 36.19%, respectively, with an average of 28.14%; number of filled grains per ear increased by 20.61% and 34.74%, respectively, with an average of 27.68%; barren tip length decreased by 77.14% and 67.39%, respectively, with an average of 72.27%; yield increased by 24.24% and 36.21%, respectively, with an average of 30.23%; and the rate of recovery from heat damage increased by 82.7% and 84.1%, respectively, with an average of 83.4%.

[0181] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 4, compared with the control CK1, the following results were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): the late-stage root and stem root sap flow increased by 36.96% and 41.64%, respectively, with an average of 39.30%; the chlorophyll content of ear leaves increased by 14.64% and 10.88%, respectively, with an average of 12.76%; SOD activity increased by 26.79% and 28.45%, respectively, with an average of 27.62%; MDA content decreased by 26.79% and 28.35%, respectively, with an average of 27.57%; and the leaf transpiration rate (Tr) increased by 26.79%, respectively. The average increase was 29.51%, with a stomatal conductance (Gs) of 45.83% and 25.81%, respectively, averaging 35.82%; the average net photosynthetic rate (Pn) of 31.94% and 37.27%, respectively, averaging 34.61%; the average thousand-grain weight of 1.17% and 0.63%, respectively, averaging 0.90%; the average number of grains per row of 0.56% and 10.29%, respectively, averaging 5.43%; the average number of filled grains per ear of 0.83% and 9.94%, respectively, averaging 5.37%; the average length of barren tips of 45.45% and 26.23%, respectively, averaging 35.84%; and the average yield of 10.1% and 11.5%, respectively, averaging 10.8%.

[0182] The high-temperature resistance conditioner provided in Example 4 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of corn under high-temperature conditions.

[0183] Application Example 5

[0184] Similar to Application Example 4, the only difference is that the high-temperature conditioner in Example 1 of Application Example 1 was replaced with the high-temperature conditioner in Example 5. The test results are shown in Tables 16-17.

[0185] Table 16 Results of Physiological and Biochemical Tests

[0186]

[0187]

[0188] Table 17 Results of Production Structure Detection

[0189]

[0190] As shown in Tables 16 and 17, after spraying the high-temperature resistance conditioner of Example 5, compared with the control CK2, the following effects were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): the late-stage root and stem root sap flow increased by 66.41% and 79.0%, respectively, with an average of 72.71%; the chlorophyll content of ear leaves increased by 3.64% and 33.74%, respectively, with an average of 18.69%; SOD activity increased by 56.34% and 44.14%, respectively, with an average of 50.24%; MDA content decreased by 28.34% and 23.09%, respectively, with an average of 25.72%; leaf transpiration rate (Tr) increased by 64.29% and 62.79%, respectively, with an average of 63.54%; and stomatal conductance (G) increased... The growth rates of s and s increased by 163.16% and 138.89% respectively, with an average of 151.03%; the net photosynthetic rate (Pn) increased by 37.25% and 20.45% respectively, with an average of 28.85%; the thousand-grain weight increased by 0.29% and 0.29% respectively, with an average of 0.29%; the number of grains per row increased by 16.54% and 28.70% respectively, with an average of 22.62%; the number of filled grains per ear increased by 18.87% and 27.84% respectively, with an average of 23.36%; the length of barren tips decreased by 77.14% and 64.58% respectively, with an average of 70.86%; the yield increased by 21.56% and 30.69% respectively, with an average of 26.13%; and the rate of recovery from heat damage increased by 82.1% and 83.4% respectively, with an average of 82.75%.

[0191] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 5, compared with the control CK1, the following results were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): root and stem sap flow increased by 38.93% and 44.65% respectively, averaging 41.79%; chlorophyll content in ear leaves increased by 11.87% and 8.44% respectively, averaging 10.16%; SOD activity increased by 25.94% and 26.60% respectively, averaging 26.27%; MDA content decreased by 26.75% and 31.62% respectively, averaging 29.19%; and leaf transpiration rate (Tr) increased by 36.36%. The percentages of grain yield and net photosynthetic rate (Pn) increased by 31.75% and 39.78%, respectively, with an average of 35.12%; stomatal conductance (Gs) increased by 48.0% and 44.83%, respectively, with an average of 46.42%; the net photosynthetic rate (Pn) increased by 31.75% and 39.78%, respectively, with an average of 35.77%; the thousand-grain weight increased by 1.47% and 0.85%, respectively, with an average of 1.16%; the number of grains per row increased by 0.88% and 3.55%, respectively, with an average of 2.22%; the number of filled grains per ear increased by 0.2% and 4.7%, respectively, with an average of 2.45%; the length of the barren tip decreased by 44.44% and 70.83%, respectively, with an average of 57.64%; and the yield increased by 11.6% and 10.5%, respectively, with an average of 11.05%.

[0192] The high-temperature resistance conditioner provided in Example 5 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of corn under high-temperature conditions.

[0193] Application Example 6

[0194] Similar to Application Example 4, the only difference is that the high-temperature conditioner in Example 1 of Application Example 1 was replaced with the high-temperature conditioner in Example 6. The test results are shown in Tables 18-19.

[0195] Table 18 Results of Physiological and Biochemical Tests

[0196]

[0197] Table 19 Results of Production Structure Detection

[0198]

[0199]

[0200] As shown in Tables 18 and 19, under high-temperature treatment, after spraying the high-temperature resistance conditioner of Example 6, compared with the control CK2, the following effects were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): the root and stem sap flow increased by 53.01% and 78.06% respectively, with an average of 65.54%; the chlorophyll content of ear leaves increased by 3.53% and 29.34% respectively, with an average of 16.5%; SOD activity increased by 47.82% and 34.71% respectively, with an average of 41.27%; MDA content decreased by 20.24% and 26.03% respectively, with an average of 23.14%; and leaf transpiration rate (Tr) increased by 57.78% and 65.85% respectively, with an average of 61.82%. Stomatal conductance (Gs) increased by 128.57% and 186.67%, respectively, with an average of 157.62%; net photosynthetic rate (Pn) increased by 40.40% and 22.03%, respectively, with an average of 31.22%; thousand-grain weight increased by 1.19% and 0.57%, respectively, with an average of 0.88%; grain number per row increased by 20.08% and 25.86%, respectively, with an average of 22.97%; number of filled grains per ear increased by 20.78% and 26.05%, respectively, with an average of 23.42%; barren tip length decreased by 75.0% and 67.39%, respectively, with an average of 71.20%; yield increased by 21.47% and 28.68%, respectively, with an average of 25.08%; and the rate of recovery from heat damage increased by 83.8% and 85.2%, respectively, with an average of 84.50%.

[0201] Under normal field temperatures, after spraying the high-temperature resistance conditioner of Example 6, compared with the control CK2, the following results were observed in maize varieties Zhengdan 958 (relatively heat-resistant) and Denghai 618 (not heat-resistant): the late-stage root and stem root sap flow increased by 38.61% and 44.4%, respectively, with an average of 41.51%; the chlorophyll content of ear leaves increased by 22.42% and 6.05%, respectively, with an average of 14.24%; SOD activity increased by 26.13% and 33.97%, respectively, with an average of 30.05%; MDA content decreased by 29.32% and 31.1%, respectively, with an average of 30.21%; and the leaf transpiration rate (Tr) increased by 39.66%, respectively. The average increase was 38.29%; stomatal conductance (Gs) increased by 52.0% and 24.24%, respectively, with an average of 38.12%; net photosynthetic rate (Pn) increased by 33.71% and 36.33%, respectively, with an average of 35.02%; thousand-grain weight increased by 1.17% and 0.65%, respectively, with an average of 0.91%; grain number per row increased by 4.32% and 7.94%, respectively, with an average of 6.13%; number of filled grains per ear increased by 7.93% and 10.28%, respectively, with an average of 9.11%; barren tip length decreased by 50.0% and 18.49%, respectively, with an average of 34.23%; and yield increased by 10.7% and 11.9%, respectively, with an average of 11.30%.

[0202] The high-temperature resistance conditioner provided in Example 6 of this invention has significant high-temperature resistance and can improve the physiological and biochemical indicators and yield structure of corn under high-temperature conditions.

[0203] Application Example 7

[0204] 1. Selection of test site and test varieties:

[0205] In 2022, a large-scale grain grower, Mr. Ding, conducted an experiment on his dryland contracted land in Beiwuji Village, Huaiyin District (a dryland grain crop demonstration base in Huaiyin District, Huai'an City, Jiangsu Province). The soil was sandy loam with medium to low fertility, and the previous crop was wheat.

[0206] The maize variety tested was Kehua 66. The maize was sown on June 23, 2022, at a planting density of 4,500 plants / mu. Field fertilizer and water management, as well as pest and disease control measures, were carried out using conventional methods.

[0207] 2. Experimental grouping and treatment methods

[0208] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-6 treatments and a control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-6 treatments and a control (CK) treatment.

[0209] Room temperature treatment - Regulators 1-6: At the corn tasseling stage, apply 100 mL / mu of heat-resistant regulator diluted in 15 kg of water and spray evenly onto the corn leaves. After spraying, proceed with conventional cultivation. Specifically, Treatment 1 used the heat-resistant regulator from Example 7 (5 mL / mu), Treatment 2 used the heat-resistant regulator from Example 8 (10 mL / mu), Treatment 3 used the heat-resistant regulator from Example 2 (15 mL / mu), Treatment 4 used the heat-resistant regulator from Example 9 (20 mL / mu), Treatment 5 used the heat-resistant regulator from Example 10 (25 mL / mu), and Treatment 6 used the heat-resistant regulator from Example 11 (30 mL / mu).

[0210] Room temperature treatment - CK treatment: At the corn tasseling stage, spray clean water evenly onto the corn leaves at a rate of 100 mL / mu, and then cultivate as usual after spraying;

[0211] High-temperature treatment - Regulator 1-6 treatments: During the corn tasseling stage, take 100 mL / mu of high-temperature resistance conditioner, dilute 100 mL of high-temperature resistance conditioner with 15 kg of water, and spray it evenly onto the rice leaves. On the fifth day after spraying the conditioner, carry out high-temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue the treatment until the corn flowers and pollinates. The high-temperature resistance conditioner used in treatments 1-6 is the same as that used in normal temperature treatment - Regulator 1-6 treatments.

[0212] High-temperature treatment - CK treatment: During the corn tasseling stage, spray clean water evenly onto the corn leaves at a dosage of 100 mL / acre. After spraying, cultivate as usual. On the fifth day after spraying, carry out high-temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue this treatment until the corn flowers and pollinates.

[0213] All the above treatment groups were implemented with three replicates, and the plot area for each replicate was 48m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0214] 3. Evaluation indicators: The yield composition indicators include plant height, number of filled grains per ear, thousand-grain weight, barren tip length, yield, and heat damage loss recovery rate. These indicators are evaluated before harvest, and the test results are shown in Table 20.

[0215] Table 20 Results of Production Structure Detection

[0216]

[0217] Table 20 shows that the plant height of maize increased with the increase of the dosage of the first conditioner. Under normal temperature conditions, the plant height increase for treatments 1 to 4 was between 0.0% and 0.19%, while the plant height increases for treatments 5 and 6 were 5.1 cm and 10.6 cm, respectively, representing increases of 2.46% and 5.11%. Under high temperature conditions, the plant height of maize increased with the dosage, with increases of 0.09% to 0.47% for treatments 1 to 4. The increases for treatments 5 and 6 reached 3.7% and 5.93%, respectively, indicating that the first conditioner had a promoting effect on maize plant height, and this effect increased with the dosage. The number of filled kernels per ear increased with the dosage between 5 mL / mu and 20 mL / mu. However, with further increases in dosage, the number of filled grains per ear tended to decrease. Treatments 5 and 6 showed a significant reduction in the number of grains per ear compared to treatments 3 and 4, with a decrease of approximately 10 grains per ear. Under normal temperature conditions, the length of barren tips in each treatment was not significantly different from the control. However, under high temperature conditions, it significantly reduced the length of barren tips. The reduction increased with increasing dosage. The thousand-grain weight increased under both high-temperature and normal temperature conditions, but the increase was not significant. Under normal temperature conditions, yield generally increased with increasing dosage, with an increase of 4% to 12%. When the dosage reached 15 mL / mu, the yield increase was over 11%. In terms of recovering heat damage losses, a dosage of 15 mL to 20 mL / mu resulted in a recovery rate of over 83%, indicating that the dosage of the first regulator should be controlled between 15 mL / mu and 20 mL / mu.

[0218] Application Example 8

[0219] 1. Selection of test site and test varieties:

[0220] In 2021, Wu Moutian, a large-scale grain grower, conducted an experiment on his contracted land in Xuexing Village, Zhuma Town, Lianshui County, Jiangsu Province (Lianshui County Demonstration Base of Jiangsu Rice and Wheat Industry Technology System). The soil fertility was moderate to high, and the previous crop was wheat.

[0221] The rice variety used in the experiment was Nanjing 45. The rice was sown on May 19, 2021, and seedlings were raised in plastic hard disk substrate. Each tray contained 80 grams of seeds. The seedlings were transplanted on June 22. The row spacing was 30cm × 10cm, with 22,000 hills per mu and 88,000 seedlings per mu. The field fertilizer and water management and pest and disease control measures were carried out in accordance with conventional methods.

[0222] 2. Experimental grouping and treatment methods

[0223] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-7 treatment and control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-7 treatment and control (CK) treatment.

[0224] Room temperature treatment - Regulators 1-7: At the heading stage, apply 100 mL / mu of high-temperature resistance regulator diluted with 15 kg of water and spray evenly onto the rice leaves. After spraying, cultivate as usual. Specifically, treatment 1 used the high-temperature resistance regulator from Example 12 (denoted as ①), treatment 2 used the high-temperature resistance regulator from Comparative Example 1 (denoted as ②), treatment 3 used the high-temperature resistance regulator from Comparative Example 2 (denoted as ③), treatment 4 used the high-temperature resistance regulator from Comparative Example 3 (denoted as ④), treatment 5 used the high-temperature resistance regulator from Comparative Example 4 (denoted as ⑤), treatment 6 used the high-temperature resistance regulator from Comparative Example 5 (denoted as ⑥), and treatment 7 used the high-temperature resistance regulator from Comparative Example 6 (denoted as ⑦).

[0225] Room temperature treatment - CK treatment: At the heading stage, spray clean water evenly onto the rice leaves at a rate of 100 mL / mu, and then cultivate as usual after spraying;

[0226] High-temperature treatment - Regulator 1-7: At the heading stage, take 100 mL / mu of high-temperature resistance conditioner, dilute 100 mL of high-temperature resistance conditioner with 15 kg of water, and spray it evenly on the rice leaves. On the fifth day after spraying the conditioner, carry out high-temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue the treatment until the rice flowers and pollinates. The high-temperature resistance conditioner used in treatments 1-6 is the same as that used in normal temperature treatment - Regulator 1-7.

[0227] High temperature treatment - CK treatment: At the beginning of heading stage, spray clean water (15 kg / mu) evenly onto the rice leaves. After spraying, cultivate as usual. On the fifth day after spraying, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to lower the temperature. At night, allow the temperature to drop naturally. Continue the treatment until the rice flowers and pollinates.

[0228] All the above treatment groups were implemented with three replicates, and the plot area for each replicate was 18m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0229] 3. Evaluation indicators: The yield composition indicators include plant height, number of filled grains per ear, thousand-grain weight, seed setting rate, yield, and heat damage loss recovery rate. These indicators are evaluated before harvest, and the test results are shown in Table 21.

[0230] Table 21 Results of Production Structure Detection

[0231]

[0232]

[0233] As shown in Table 12, different compositions of the first growth regulator all increased plant height. Under high temperature conditions, the increase in plant height ranged from 0.19% to 7.73%, while under normal temperature conditions, the increase ranged from 0.19% to 3.53%. Under high temperature conditions, the different proportions had significant effects on the number of grains per ear, with increases ranging from 11.3% to 22.9%. Treatment 1 (GA3 / NAA / 6BA = 2.5 / 1.5 / 1.3) increased the number of grains per ear by 22.9% compared to the control; Treatment 5 increased by 21.4%, and the others all increased by less than 20%; while Treatment 7 only increased by 11.3%. The overall change in thousand-grain weight was not significant. Under normal temperature conditions, all treatments increased compared to the control, but under high temperature conditions, except for Treatments 1 and 5, the thousand-grain weight of the other treatments decreased compared to the control. Under high temperature conditions, all treatments increased yield compared to the control, but the increases varied considerably. Treatments 1, 3, and 5 increased yield by 25.51%, 20.86%, and 18.5%, respectively, while the remaining treatments showed increases between 7% and 10%. Under normal temperature conditions, except for treatment 1, which increased yield by 12.71% compared to the control, the increases in yield for the other treatments were all less than 10%. In terms of recovering yield losses from heat damage, treatment 1 achieved 83.96%, treatments 3 and 5 achieved 68.7% and 60.92%, respectively, and the rest were between 20% and 35%. Treatment 1 (GA3 / NAA / 6BA = 2.5 / 1.5 / 1.3) showed the most significant effects in increasing yield and resisting heat damage. This indicates that different ratios of GA3 / NAA / 6BA are closely related to ensuring increased yield under conditions of resisting heat damage and without heat damage, and that selecting a reasonable GA3 / NAA / 6BA ratio has a significant impact on product performance.

[0234] Application Example 9

[0235] The high-temperature modifiers obtained in Example 12 were randomly divided into 3 groups. The high-temperature modifiers obtained in the first group were stored at low temperature (minus 18°C) for 6 months (referred to as treatment 1). The high-temperature modifiers obtained in the second group were stored at high temperature (50°C) for 6 months (referred to as treatment 2). The high-temperature modifiers obtained in the third group were stored at room temperature (25°C) for 6 months (referred to as treatment 3). The appearance, including color and precipitation, packaging appearance, weight and volume were observed. The results are shown in Table 22.

[0236] Table 22 Product Changes Under Different Temperatures

[0237] Process 1 The packaging appearance remained unchanged, the color remained unchanged, there was no sediment, the volume remained unchanged, and the appearance was no different from that stored at room temperature. Process 2 The packaging appearance remained unchanged, the color remained unchanged, there was no sediment, the volume remained unchanged, and the appearance was no different from that stored at room temperature. Process 3 Light brownish-red, transparent liquid, without sediment, and unchanged in volume, weight, or packaging appearance.

[0238] As can be seen from Table 22, the high-temperature regulator provided by this invention showed no change in appearance or content of main components after being stored continuously for 6 months at low temperature (minus 18°C), high temperature (50°C), and room temperature, respectively, indicating good stability.

[0239] Application Example 10

[0240] The stability of the high-temperature resistance regulators in Examples 13-14 and Comparative Examples 7-8 was observed after 5 hours, 10 days, and 30 days of preparation; the stability was also observed after 2 years of preparation, and the contents of GA3, NAA, and 6-BA were detected. The results are shown in Table 23.

[0241] Table 23. Stability of GA3, NAA, and 6-BA contents at different fulvic acid dosages.

[0242]

[0243] Note: If no precipitation occurs, no color change, no weight change, and the content remains stable during the observation process, it is considered stable.

[0244] As can be seen from Table 23, when the concentration of fulvic acid in the first regulator of the high-temperature regulator provided by the present invention is 0.5 to 0.75 g / 100 mL, the appearance and physical properties of the high-temperature regulator and the contents of GA3, NAA, and 6-BA are stable for at least two years.

[0245] Application Example 11

[0246] The stability of the high-temperature resistance regulators in Examples 2, 15-16 and Comparative Examples 9-11 was observed after 5 hours, 10 days, 30 days and 2 years. The results are shown in Table 24.

[0247] Table 24 Stability of different treatment methods

[0248]

[0249] As can be seen from Table 24, the concentration of the first regulator GA3+NAA+6-BA in the high-temperature resistant regulator provided by the present invention cannot exceed 20% in order to ensure the stability of the product. When preparing the first regulator, the amount of solvent ethanol must not be less.

[0250] Application Example 12

[0251] 1. Selection of test site and test varieties:

[0252] In 2022, Wu Moutian, a large-scale grain grower, conducted an experiment on his contracted land in Xuexing Village, Zhuma Town, Lianshui County, Jiangsu Province (Lianshui County Demonstration Base of Jiangsu Rice and Wheat Industry Technology System). The soil fertility was moderate to high, and the previous crop was wheat.

[0253] The rice variety tested was Nanjing 9108. The rice was sown on May 15, 2022, and seedlings were raised in plastic hard disk substrate. Each tray contained 80 grams of seeds. The seedlings were transplanted on June 21. The row spacing was 30cm × 10cm, with 22,000 hills per mu and a basic seedling density of 88,000 per mu.

[0254] 2. Experimental grouping and treatment methods

[0255] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-7 treatment and control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-7 treatment and control (CK) treatment.

[0256] Room temperature treatment - Regulators 1-7: At the heading stage, 100 mL of high-temperature resistance regulator per mu was diluted with 15 kg of water and sprayed evenly onto the rice leaves. After spraying, conventional cultivation was carried out. Among them, treatment 1 used comparative example 12 high-temperature resistance regulator (referred to as ①), treatment 2 used comparative example 13 high-temperature resistance regulator (②), treatment 3 used example 2 high-temperature resistance regulator (③), treatment 4 used comparative example 14 high-temperature resistance regulator (④), treatment 5 used comparative example 15 high-temperature resistance regulator (⑤), treatment 6 used comparative example 16 high-temperature resistance regulator (referred to as ⑥), and treatment 7 used comparative example 17 high-temperature resistance regulator (referred to as ⑦).

[0257] Room temperature treatment - CK treatment: At the heading stage, spray clean water evenly onto the rice leaves at a rate of 100 mL / mu, and then cultivate as usual after spraying;

[0258] High temperature treatment - regulator 1 to 7 treatment: At the beginning of heading, the treatment is carried out according to the normal temperature treatment - regulator treatment. On the fifth day after the conditioner is sprayed, high temperature treatment is carried out: a plastic film shed is erected to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilation is carried out to lower the temperature. At night, the temperature is allowed to drop naturally. This treatment is carried out continuously until the end of rice flowering and pollination.

[0259] High temperature treatment - CK treatment: At the beginning of heading stage, spray clean water evenly onto the rice leaves at a dosage of 100mL / mu. On the fifth day after spraying, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue the treatment until the rice flowers and pollinates.

[0260] All the above treatment groups were designed with three replicates, with each treatment plot having an area of ​​18m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0261] 3. The rice was investigated, and the following indicators were investigated: (1) plant height (cm); (2) number of panicles per mu; (3) number of grains per panicle; (4) thousand-grain weight (g); (5) plot yield (kg / mu); (6) heat damage recovery rate ((yield of high temperature compound spray treatment - yield of high temperature control) / (yield of normal temperature control - yield of high temperature control) * 100). Among them, the physiological and biochemical indicators were sampled and tested 30 days after the conditioner treatment; the yield composition traits were investigated before harvest, and the test results are shown in Table 25.

[0262] Table 25 Results of Production Structure Detection

[0263]

[0264]

[0265] Table 25 shows that high-temperature treatment slightly increased plant height, but the plant height tended to decrease as the proportion of foxtail grass increased. Treatments ① and ② increased plant height by 1.7 cm and 1.9 cm respectively compared to treatment ⑧ (high-temperature control), representing increases of 2.1% and 2.3% respectively. At room temperature, plant height did not change significantly, but it tended to decrease as the proportion of foxtail grass increased. Treatments ①, ②, ③, and ④ increased plant height by 2.34 cm, 1.84 cm, 1.04 cm, and -0.86 cm respectively compared to the room-temperature control. The proportions of foxtail grass varied from 0.0 / 0.5 / 1.0 / 2.0. Different proportions significantly affected the grain / ear ratio and seed setting rate, with treatment ③ showing the best grain / ear ratio and seed setting rate, increasing by 139.4% and 98.2% respectively compared to the high-temperature control, and by 7.07% and 7.21% compared to the normal-temperature control. Under normal temperature, treatments ①, ②, and ③ showed yield increases of 13.5%, 12.1%, 11.3%, and 8.75% respectively compared to treatment ⑧ (normal-temperature control), with a clear trend of decreasing yield increase as the proportion of foxtail grass increased. The indicator of heat damage loss recovery rate clearly shows that treatment ③, with a foxtail grass / prunella vulgaris / coptis chinensis ratio of 1.0 / 0.5 / 0.25, had the best recovery rate, achieving 83.64% loss recovery. However, the loss recovery rate began to decrease as the proportion of foxtail grass increased. Treatment ④ (2.0 / 0.5 / 0.25) achieved a loss recovery rate of 78.1%, a decrease of 5.54 percentage points (6.6%) compared to treatment ③. Prunella vulgaris and Coptis chinensis played a crucial role in mitigating heat damage losses. Treatments ⑥ and ⑦, lacking Prunella vulgaris or Coptis chinensis, resulted in losses decreasing by 19.44 and 14.14 percentage points respectively compared to treatment ③, representing decreases of 23.24% and 16.91% respectively.

[0266] Application Example 13

[0267] 1. Selection of test site and test varieties:

[0268] In 2020, Wu Moutian, a large-scale grain grower, conducted an experiment on his contracted land in Xuexing Village, Zhuma Town, Lianshui County, Jiangsu Province (Lianshui County Demonstration Base of Jiangsu Rice and Wheat Industry Technology System). The soil fertility was moderate to high, and the previous crop was wheat.

[0269] The rice variety tested was Nanjing 9108. The rice was sown on May 18, 2020, and seedlings were raised in plastic hard disk substrate. Each tray contained 80 grams of seeds. The seedlings were transplanted on June 24. The row spacing was 30cm × 10cm, with 22,000 hills per mu and a basic seedling density of 88,000 per mu.

[0270] 2. Experimental grouping and treatment methods

[0271] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-7 treatment and control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-7 treatment and control (CK) treatment.

[0272] Room temperature treatment - Regulators 1-7: At the heading stage, 100 mL of high-temperature resistance regulator was diluted with 15 kg of water and sprayed evenly onto the rice leaves. After spraying, conventional cultivation was carried out. Among them, treatment 1 used the high-temperature resistance regulator of Example 17 (denoted as ①), treatment 2 used the high-temperature resistance regulator of Example 18 (②), treatment 3 used the high-temperature resistance regulator of Example 19 (③), treatment 4 used the high-temperature resistance regulator of Example 20 (④), treatment 5 used the high-temperature resistance regulator of Example 21 (⑤), treatment 6 used the high-temperature resistance regulator of Example 22 (denoted as ⑥), and treatment 7 used the high-temperature resistance regulator of Example 23 (denoted as ⑦).

[0273] Room temperature treatment - CK treatment: At the heading stage, spray water on the rice leaves at a rate of 100 mL / mu and cultivate in a conventional manner.

[0274] High temperature treatment - regulator 1 to 7 treatment: At the beginning of heading, the treatment is carried out according to the normal temperature treatment - regulator treatment. On the fifth day after the conditioner is sprayed, high temperature treatment is carried out: a plastic film shed is erected to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilation is carried out to lower the temperature. At night, the temperature is allowed to drop naturally. This treatment is carried out continuously until the end of rice flowering and pollination.

[0275] High temperature treatment - CK treatment: At the beginning of heading stage, spray water on the rice leaves at a dosage of 100mL / mu. On the fifth day after spraying, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to cool down. At night, allow the temperature to cool down naturally. Continue the treatment until the rice flowers and pollinates.

[0276] 3. Before harvest, the yield of rice and the rate of recovery from heat damage were investigated, and the results are shown in Table 26.

[0277] Table 26 Results of Yield and Recovery Rate of Heat Damage

[0278]

[0279] As shown in Table 26, the addition of organophosphonic acid during extraction significantly affects the recovery rate from high-temperature heat damage. Treatment ⑤ (without any organophosphonic acid added during extraction) had a recovery rate of only 31.6%, while the average recovery rate for treatments ①②③④ was 77.65%. Different organophosphonic acids showed variations, but ethylene diphosphonic acid was preferred, achieving a recovery rate of 82.5%. Furthermore, even at room temperature, there was a 10.56% yield increase, while the yield increases for the other three organophosphonic acids were 8.63%, 8.74%, and 7.58%, respectively. Extraction time had a highly significant impact on the results. Treatment ⑥ (extraction time of 12 hours) had a high-temperature damage recovery rate of only 64.7%, with an average yield increase of only 5.53% at room temperature. In contrast, treatments ① (extraction time of 24 hours) and ⑦ (extraction time of 48 hours) had recovery rates of 82.5% and 82.6%, respectively, and average yield increases of 10.56% and 10.5% at room temperature, respectively. This clearly demonstrates that an extraction time of up to 24 hours is perfectly adequate.

[0280] Application Example 14

[0281] 1. Selection of test site and test varieties:

[0282] In 2022, a large-scale grain grower, Mr. Ding, conducted an experiment on his dryland contracted land in Beiwuji Village, Huaiyin District (a dryland grain crop demonstration base in Huaiyin District, Huai'an City, Jiangsu Province). The soil was sandy loam with medium to low fertility, and the previous crop was wheat.

[0283] The maize variety tested was Kehua 66. The maize was sown on June 23, 2022, at a planting density of 4,500 plants / mu. Field fertilizer and water management, as well as pest and disease control measures, were carried out using conventional methods.

[0284] 2. Experimental grouping and treatment methods

[0285] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-6 treatments and a control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-6 treatments and a control (CK) treatment.

[0286] Room temperature treatment - Regulators 1-6: During the corn tasseling stage, apply 100 mL / mu of heat-resistant regulator diluted in 15 kg of water and spray evenly onto the corn leaves. After spraying, proceed with conventional cultivation. Specifically, Treatment 1 used the heat-resistant regulator from Example 24 (20 mL / mu), Treatment 2 used the heat-resistant regulator from Example 25 (10 mL / mu), Treatment 3 used the heat-resistant regulator from Example 26 (40 mL / mu), Treatment 4 used the heat-resistant regulator from Example 2 (50 mL / mu), Treatment 5 used the heat-resistant regulator from Example 27 (60 mL / mu), and Treatment 6 used the heat-resistant regulator from Example 28 (70 mL / mu).

[0287] Room temperature treatment - CK treatment: At the corn tasseling stage, take the high temperature resistance conditioner obtained in Comparative Example 1 at a dosage of 100 mL / mu, dilute 100 mL of high temperature resistance conditioner with 15 kg of water, and spray it evenly on the corn leaves. After spraying, cultivate as usual.

[0288] High-temperature treatment - Regulator 1-6 treatments: During the corn tasseling stage, take 100 mL / mu of high-temperature resistance conditioner, dilute 100 mL of high-temperature resistance conditioner with 15 kg of water, and spray it evenly onto the rice leaves. On the fifth day after spraying the conditioner, carry out high-temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue the treatment until the corn flowers and pollinates. The high-temperature resistance conditioner used in treatments 1-6 is the same as that used in normal temperature treatment - Regulator 1-6 treatments.

[0289] High temperature treatment - CK treatment: At the corn tasseling stage, take the high temperature resistance conditioner obtained in Comparative Example 1 at a dosage of 100 mL / mu, dilute 100 mL of the high temperature resistance conditioner with 15 kg of water, and spray it evenly on the corn leaves. After spraying, carry out conventional cultivation. On the fifth day after spraying the conditioner, carry out high temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. At night, allow the temperature to drop naturally. Continue the treatment until the corn flowers and pollinates.

[0290] All the above treatment groups were implemented with three replicates, and the plot area of ​​each replicate was 48m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0291] 3. The corn was investigated, and the following indicators were examined: in terms of yield composition, the plant height, number of filled kernels / ear, thousand-kernel weight, barren head length, yield, and heat damage recovery rate. Among them, when investigating the corn, the physiological and biochemical indicators were sampled and tested 30 days after the conditioner treatment; the ear traits were examined before harvest, and the test results are shown in Table 27.

[0292] Table 27 Results of Production Structure Detection

[0293]

[0294] As shown in Table 27, the plant height of maize decreased with increasing dosage. Under normal temperature conditions, the plant height of treatments 1-4 increased by 0.0% to -1.39% compared to the control, while treatments 5 and 6 decreased by 4.0 cm and 5.61 cm respectively, representing decreases of 1.92% and 2.69%. Under high temperature conditions, the plant height of treatments 1-4 increased by 1.25% to 0.33% compared to the control. Treatments 5 and 6 decreased by 0.91% and 2.29% respectively, indicating that the combined herbal extract and water extract had a certain inhibitory effect on maize plant height, and this effect increased with increasing dosage. Under high temperature conditions, with dosages ranging from 20 mL / mu to 50 mL / mu, the number of filled kernels per ear increased with increasing dosage. The number of filled kernels per ear in treatments 1-4 increased by 12.38%, 21.64%, 27.51%, and 29.81% respectively compared to the control. However, the increase tended to decrease with further increases in dosage. Under normal temperature conditions, the number of filled grains per ear was significantly increased compared to the control when the dosage was between 20 mL / mu and 50 mL / mu, with an increase of 10.88% to 11.98%. However, the change was significant when the dosage was further increased. Treatment 5 (60 mL / mu) showed a 4.9% increase in the number of filled grains per ear compared to the control, while treatment 6 (70 mL / mu) showed a -5.37% decrease. The length of the barren head was significantly reduced in all treatments compared to the control. The thousand-grain weight did not change much under both high-temperature and normal temperature conditions. The yield was increased compared to the control under normal temperature conditions, but the yield increase decreased significantly with the increase in dosage. The increases in treatments 1 to 6 compared to the control were 12.1%, 11.9%, 11.9%, 11.7%, 6.5%, and 4.1%, respectively. Under high-temperature treatment, all treatments resulted in increased yields compared to the control. The increases from treatment 1 to 6 were 15.7%, 24.8%, 30.8%, 31.2%, 31.4%, and 30.9%, respectively. In terms of recovering from heat damage, the recovery rates for treatments 1 to 6 were 41.2%, 65.3%, 80.9%, 82.1%, 82.5%, and 81.1%, respectively. With a dosage of 40 mL to 70 mL per acre, the recovery rate was over 80%.

[0295] Application Example 15

[0296] 1. Selection of test site and test varieties:

[0297] In 2020, Wu Moutian, a large-scale grain grower, conducted an experiment on his contracted land in Xuexing Village, Zhuma Town, Lianshui County, Jiangsu Province (Lianshui County Demonstration Base of Jiangsu Rice and Wheat Industry Technology System). The soil fertility was moderate to high, and the previous crop was wheat.

[0298] The rice variety tested was Nanjing 9108. The rice was sown on May 18, 2020, and seedlings were raised in plastic hard disk substrate. Each tray contained 80 grams of seeds. The seedlings were transplanted on June 24. The row spacing was 30cm × 10cm, with 22,000 hills per mu and a basic seedling density of 88,000 per mu.

[0299] 2. Experimental grouping and treatment methods

[0300] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-7 treatment and control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-7 treatment and control (CK) treatment.

[0301] Room temperature treatment - Regulators 1-7: At the heading stage, 100 mL of high-temperature resistance regulator was diluted with 15 kg of water and sprayed evenly onto the rice leaves. After spraying, conventional cultivation was carried out. Among them, treatment 1 used the high-temperature resistance regulator of Example 2 (denoted as ①), treatment 2 used the high-temperature resistance regulator of Comparative Example 18 (②), treatment 3 used the high-temperature resistance regulator of Comparative Example 19 (③), treatment 4 used the high-temperature resistance regulator of Comparative Example 20 (④), treatment 5 used the high-temperature resistance regulator of Comparative Example 21 (⑤), treatment 6 used the high-temperature resistance regulator of Comparative Example 22 (denoted as ⑥), and treatment 7 used the high-temperature resistance regulator of Comparative Example 23 (denoted as ⑦).

[0302] Room temperature treatment - CK treatment: At the heading stage, spray water on the rice leaves at a rate of 100 mL / mu and cultivate in a conventional manner.

[0303] High temperature treatment - regulator 1 to 7 treatment: At the beginning of heading, the treatment is carried out according to the normal temperature treatment - regulator treatment. On the fifth day after the conditioner is sprayed, high temperature treatment is carried out: a plastic film shed is erected to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilation is carried out to lower the temperature. At night, the temperature is allowed to drop naturally. This treatment is carried out continuously until the end of rice flowering and pollination.

[0304] High temperature treatment - CK treatment: At the beginning of heading stage, spray water on the rice leaves at a dosage of 100mL / mu. On the fifth day after spraying, carry out high temperature treatment: build a plastic film shed to raise the temperature and ensure that the temperature inside the shed is above 34℃ during the day. When the temperature is ≥37℃, ventilate to cool down. At night, allow the temperature to cool down naturally. Continue the treatment until the rice flowers and pollinates.

[0305] 3. Before rice harvest, the yield of rice and the rate of recovery from heat damage were investigated, and the results are shown in Table 28.

[0306] Table 28 Results of Yield and Recovery Rate of Heat Damage

[0307]

[0308] Table 28 shows that adding Pix, vitamins (VC, VB1, VB4), and amino acids (threonine, serine) to the formula increases heat resistance and yield. Treatments ②, ③, and ④ showed loss recovery rates of 78.1%, 70.9%, and 60.4%, respectively, representing decreases of 6.01%, 14.68%, and 27.32% compared to treatment ①. This indicates that the amino acid combination (serine + threonine) had the greatest effect, followed by the vitamin combination (VC + VB1 + VB4), and lastly Pix. Spraying Pix alone or the vitamin combination (VC + VB4) alone... 11 Both VB4 and amino acid combinations (threonine + serine) have certain yield-increasing and heat-resistant effects. The yield increases at room temperature were 3.34%, 5.53%, and 6.05%, respectively; the loss reduction rates under high-temperature treatment were 41.6%, 44.7%, and 52.6%, respectively. The amino acid combination had the strongest effect, followed by the vitamin combination, and lastly, PIX.

[0309] Application Example 16

[0310] 1. Selection of test site and test varieties:

[0311] In 2022, a large-scale grain grower, Mr. Ding, conducted an experiment on his dryland contracted land in Beiwuji Village, Huaiyin District (a dryland grain crop demonstration base in Huaiyin District, Huai'an City, Jiangsu Province). The soil was sandy loam with medium to low fertility, and the previous crop was wheat.

[0312] The maize variety tested was Kehua 66. The maize was sown on June 23, 2022, at a planting density of 4,500 plants / mu. Field fertilizer and water management, as well as pest and disease control measures, were carried out using conventional methods.

[0313] 2. Experimental grouping and treatment methods

[0314] The experimental groups were divided into room temperature treatment and high temperature treatment groups. The room temperature treatment was randomly divided into regulator 1-6 treatments and a control (CK) treatment; the high temperature treatment was also randomly divided into regulator 1-6 treatments and a control (CK) treatment.

[0315] Room temperature treatment - Regulators 1-6: During the corn tasseling stage, apply 100 mL / mu (approximately 667 square meters) of heat-resistant conditioner diluted in 15 kg of water and spray evenly onto the corn leaves. After spraying, proceed with conventional cultivation. Specifically, Treatment 1 used the heat-resistant conditioner from Example 2 (referred to as ①), Treatment 2 used the heat-resistant conditioner from Comparative Example 24 (referred to as ②), Treatment 3 used the heat-resistant conditioner from Comparative Example 25 (referred to as ③), Treatment 4 used the heat-resistant conditioner from Comparative Example 26 (referred to as ④), Treatment 5 used the heat-resistant conditioner from Comparative Example 27 (referred to as ⑤), and Treatment 6 used the heat-resistant conditioner from Comparative Example 28 (referred to as ⑥).

[0316] Room temperature treatment - CK treatment: At the corn tasseling stage, spray clean water evenly onto the corn leaves at a rate of 100 mL / mu, and then cultivate as usual after spraying;

[0317] High-temperature treatment - Regulator 1-6 treatments: During the corn tasseling stage, take 100 mL / mu of high-temperature resistance conditioner, dilute 100 mL of high-temperature resistance conditioner with 15 kg of water, and spray it evenly onto the rice leaves. On the fifth day after spraying the conditioner, carry out high-temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue the treatment until the corn flowers and pollinates. The high-temperature resistance conditioner used in treatments 1-6 is the same as that used in normal temperature treatment - Regulator 1-6 treatments.

[0318] High-temperature treatment - CK treatment: During the corn tasseling stage, spray clean water evenly onto the corn leaves at a dosage of 100 mL / acre. After spraying, cultivate as usual. On the fifth day after spraying, carry out high-temperature treatment: build a plastic film greenhouse to raise the temperature and ensure that the temperature inside the greenhouse is above 35℃ during the day. When the temperature is ≥38℃, ventilate to lower the temperature. Allow the temperature to drop naturally at night. Continue this treatment until the corn flowers and pollinates.

[0319] All the above treatment groups were implemented with three replicates, and the plot area for each replicate was 48m². 2 Field fertilizer and water management, as well as pest and disease control measures, are the same as those for conventional high-yield cultivation.

[0320] 3. The corn was examined, and the following indicators were examined: in terms of yield composition, the plant height, number of filled kernels / ear, thousand-kernel weight, barren head length, yield, and heat damage loss recovery rate. Among them, the physiological and biochemical indicators were sampled and tested 30 days after the conditioner treatment; the ear traits were examined before harvest, and the test results are shown in Table 29.

[0321] Table 29 Results of Production Structure Detection

[0322]

[0323] As shown in Table 29, removing the combined extract of the herbal medicines (Foxtail Grass / Prunella Vulgaris / Coptis chinensis) from the formulation of Example 2 significantly reduced the rate of heat damage recovery after high-temperature treatment. Treatment ② showed a 54.58% decrease compared to Treatment ①, but the yield increase at room temperature was the largest, reaching 12.1%, which was 2.0% higher than Treatment ①. The yield increase was 22.2%. This indicates that the herbal extract has a significant effect on improving the rate of heat damage recovery. Removing the hormone components (GA3 / NAA / 6BA) from the formulation of Example 2 reduced the loss recovery rate, with Treatment ③ showing a 22.1% decrease compared to Treatment ①. However, the yield increase at room temperature also decreased significantly, with Treatment ③ showing a 63.64% decrease compared to Treatment ①. The results showed that the hormone components (GA3 / NAA / 6BA) had a significant yield-increasing effect at room temperature. Removing the (pix + vitamin components + amino acid components) components from the formulation in Example 2 had little effect on mitigating heat damage losses; treatment ④ only reduced the heat damage loss rate by 2.56% compared to treatment ①. However, the yield increase under room temperature conditions was significantly lower than the control (treatment ⑦), with treatment ④ decreasing by 45.45% compared to treatment ①. This indicates that the removed components had a significant yield-increasing effect at room temperature. Spraying the plant extract alone reduced heat damage losses by 43.7% under high-temperature treatment, but reduced yield by 4.5% at room temperature. This was mainly due to a decrease in thousand-grain weight and the number of grains per ear. Plant height decreased by 2.96%, indicating an inhibitory effect on maize growth. Spraying the hormone components alone reduced heat damage losses by only 20.1% under high-temperature treatment. However, it had a significant yield-increasing effect at room temperature, increasing yield by 6.2% compared to the control (treatment ⑦). It also promoted plant height. The plant height increased by 3.7 cm at room temperature and by 6.4 cm under high temperature treatment; the extract concentrate of the herbal composition of this invention has a significant synergistic effect with the plant hormone components.

[0324] As can be seen from the above, the high-temperature resistance regulator provided by this invention can mitigate the yield reduction and quality decline caused by the decrease in rice and corn seed setting rate, number of grains per ear, and grain weight due to high temperature damage, and can recover the losses caused by high temperature damage and increase yield.

[0325] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A high-temperature resistant conditioning agent, characterized in that, Based on volume, it consists of 20-70 parts of concentrated extract of traditional Chinese medicine composition, 15-20 parts of first conditioning agent and 15-20 parts of second conditioning agent; The herbal composition comprises *Setaria viridis*, *Prunella vulgaris*, and *Coptis chinensis*; the solvent for the concentrated extract of the herbal composition is water and organophosphonic acid, with a density of 1.03~1.04 g / cm³. 3 ; The first conditioning agent is GA3, NAA, 6BA, fulvic acid, and a solvent; the solvent is an alcohol, an acid, and water; the mass percentage of fulvic acid in the first conditioning agent is 0.5%~0.75%; the mass ratio of GA3, NAA, and 6BA in the first conditioning agent is (2.2~2.5):1.5:1.3; According to the mass fractions, the second conditioning agent consists of 1.8-2.7 parts of phytositol, 0.8-2.4 parts of threonine, 0.8-2.4 parts of serine, 0.45-1.8 parts of vitamin C, 0.45-1.8 parts of vitamin B1, 0.45-1.8 parts of vitamin B4 and 15-20 parts of water; The organophosphonic acid is one or more selected from oxalic acid, ethylenediaminetetramethylene phosphoric acid, 2-chloroethyl phosphoric acid and hydroxyethylidene diphosphate; The method for preparing the extract concentrate of the traditional Chinese medicine composition includes: mixing foxtail grass, prunella vulgaris and coptis chinensis to obtain the traditional Chinese medicine composition; mixing water, organophosphonic acid and the traditional Chinese medicine composition, extracting and concentrating to obtain the extract concentrate of the traditional Chinese medicine composition; The extraction temperature is 50~60℃, and the time is 24~48h; The mass ratio of foxtail grass, prunella vulgaris, and coptis chinensis is 1.0:0.5:0.25; The mass ratio of the traditional Chinese medicine composition, water, and organophosphonic acid is 2.0:12:0.5; The heat-resistant conditioning agent is targeted at rice and / or corn.

2. The high-temperature conditioning agent according to claim 1, characterized in that, The alcohol includes ethanol; the acid includes hydrochloric acid.

3. The high-temperature conditioning agent according to claim 1, characterized in that, The grain size of the foxtail grass, prunella vulgaris, and coptis chinensis is 60-80 mesh.

4. The method for preparing the high-temperature conditioning agent according to any one of claims 1 to 3, characterized in that, The process includes the following steps: mixing GA3, NAA, and an alcohol in a solvent to obtain an alcohol solution; Mix 6BA with the acid in the solvent to obtain an acid solution; The alcohol solution, acid solution, fulvic acid, and water in the solvent are mixed to obtain the first conditioning agent; The first conditioning agent, the second conditioning agent, and the concentrated extract of the traditional Chinese medicine composition are mixed to obtain the high-temperature resistant conditioning agent.

5. The application of the heat-resistant conditioner according to any one of claims 1 to 3 or the heat-resistant conditioner obtained by the preparation method according to claim 4 in crop cultivation; The crops mentioned are rice and / or corn.

6. The application according to claim 5, characterized in that, Apply a high-temperature resistance conditioner diluted in water 5 days before the rice enters the booting stage or before high-temperature damage occurs. The application method includes foliar spraying; the application amount of the heat-resistant conditioner is 100 mL / acre; when applying with water, the mass-to-volume ratio of water to heat-resistant conditioner is 15 kg: 100 mL.

7. The application according to claim 5, characterized in that, Apply a high-temperature resistance conditioner diluted with water 5 days before the corn reaches the large trumpet stage or before the occurrence of high-temperature damage. The application method includes foliar spraying; the application amount of the heat-resistant conditioner is 100 mL / acre; when applying with water, the mass-to-volume ratio of water to heat-resistant conditioner is 15 kg: 100 mL.

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