Oral care compositions comprising hyaluronic acid and polyphosphate and uses thereof

Abstract

The application discloses an oral care composition containing hyaluronic acid and polyphosphate and application thereof, and relates to the technical field of oral care, and specifically discloses an oral care composition containing hyaluronic acid and polyphosphate, which comprises: 1) hyaluronic acid; 2) polyphosphate; and 3) an orally acceptable carrier; wherein the polyphosphate is one or a combination of two or more of pyrophosphate, tripolyphosphate or hexametaphosphate; and the pH value of the oral care composition is 6-9. The hyaluronic acid in the oral care composition can improve the stability of the polyphosphate in the composition, and the hyaluronic acid has the effect of improving the stability of the polyphosphate in the oral care composition; and the oral care composition containing hyaluronic acid and polyphosphate has a good effect on tooth whitening.

Description

Technical Field

[0001] This invention relates to the field of oral care technology, and in particular to an oral care composition comprising hyaluronic acid and polyphosphate and its application. Background Technology

[0002] Polyphosphates such as pyrophosphate, tripolyphosphate, and hexametaphosphate have a long history of use in oral care compositions. They are commonly used as pH adjusters in product formulations or to deliver anti-tartar and teeth whitening effects. However, the stability of polyphosphates is affected by the surrounding environment. For example, the specification of Chinese patent CN97199983.X describes on page 1, line 25: "Some polyphosphates, especially linear polyphosphates with an average chain length greater than 4, can significantly react with most ionic fluoride sources in some oral compositions and change the pH of the oral composition. This reaction impairs the ability of the oral composition to provide stable ionic fluoride and polyphosphates to the oral surface." For example, the study results of the literature "The Hydrolysis of The Condensed Phosphates" (Can. Journal of Chemistry. 1954, 32, 42-47) proved that the stability of pyrophosphate and tripolyphosphate is affected by pH. Figures 1 and 2 show that the hydrolysis rate of pyrophosphate increases as the pH decreases, while tripolyphosphate is most stable between pH 9 and 10.

[0003] Fluoride is the most commonly used anti-caries active ingredient in oral care. The new national standard stipulates that the pH range of toothpaste should be 5.5-10, and different bases are suitable for different pH values. For example, wintergreen oil is a very popular fragrance in toothpaste, and its main component, methyl salicylate, has a stable pH range of 6-6.5. Therefore, it is necessary to develop a method to improve the stability of polyphosphates in the suitable pH environment of oral care compositions. Summary of the Invention

[0004] The first technical problem to be solved by the present invention is to provide an oral care composition comprising hyaluronic acid and polyphosphate; wherein the hyaluronic acid in the oral care composition can improve the stability of polyphosphate in the pH range of 6-9.

[0005] The second technical problem to be solved by the present invention is to provide an application of hyaluronic acid in oral care compositions to improve the stability of polyphosphates.

[0006] The third technical problem to be solved by the present invention is to provide an application of an oral care composition containing hyaluronic acid and polyphosphate in teeth whitening.

[0007] To solve the first technical problem mentioned above, the present invention adopts the following technical solution:

[0008] An oral care composition comprising hyaluronic acid and polyphosphate, comprising:

[0009] 1) Hyaluronic acid;

[0010] 2) Polyphosphates;

[0011] 3) Oral-acceptable carriers;

[0012] The polyphosphate is one or a combination of two or more of pyrophosphate, tripolyphosphate or hexametaphosphate;

[0013] The oral care composition has a pH value of 6-9.

[0014] In some embodiments, the polyphosphate is an acid salt of polyphosphate.

[0015] In some embodiments, the polyphosphate is one or a combination of two or more of the potassium, ammonium, and sodium salts of polyphosphate.

[0016] In some embodiments, the polyphosphate is one or a combination of two or more of sodium pyrophosphate, potassium pyrophosphate, sodium tripolyphosphate, potassium tripolyphosphate, sodium hexametaphosphate, and potassium hexametaphosphate.

[0017] In some embodiments, the polyphosphate accounts for 0.5-10% by weight in the oral care composition.

[0018] In some preferred embodiments, the polyphosphate accounts for 1.0-5.0% by weight in the oral care composition.

[0019] In some embodiments, the hyaluronic acid is one or a combination of two or more of the sodium, potassium, and ammonium salts of hyaluronic acid.

[0020] In some embodiments, the hyaluronic acid accounts for 0.005-1% of the mass of the oral care composition.

[0021] In some preferred embodiments, the hyaluronic acid accounts for 0.01-0.5% of the mass of the oral care composition.

[0022] In some preferred embodiments, the hyaluronic acid accounts for 0.05-0.2% by mass of the oral care composition.

[0023] In some embodiments, the pH value of the oral care composition is 6.5-8.5.

[0024] In some embodiments, the oral care composition further includes a fluoride ion source.

[0025] In some preferred embodiments, the fluoride ion source is one or more of stannous fluoride, sodium fluoride, potassium fluoride, amine fluoride, sodium monofluorophosphate, and zinc fluoride.

[0026] In some preferred embodiments, the fluoride ions in the fluoride ion source account for 0.05-0.2% by weight in the oral care composition.

[0027] In some embodiments, the oral care composition includes toothpaste, gel, oral care solution, liquid toothpaste, or tooth powder.

[0028] To address the second technical problem mentioned above, the present invention provides an application of hyaluronic acid in oral care compositions to improve the stability of polyphosphates.

[0029] To address the third technical problem mentioned above, the present invention provides an application of an oral care composition comprising hyaluronic acid and polyphosphate in teeth whitening.

[0030] Unless otherwise specified, all raw materials used in this invention can be obtained commercially, and the equipment used in this invention can be conventional equipment in the relevant field or refer to existing technology in the relevant field.

[0031] Beneficial effects of the present invention

[0032] This invention provides an oral care composition comprising hyaluronic acid and polyphosphate. The hyaluronic acid in the oral care composition can improve the stability of the polyphosphate in the composition. This invention also provides an application of hyaluronic acid in improving the stability of polyphosphate in oral care compositions. Furthermore, the oral care composition comprising hyaluronic acid and polyphosphate of this invention also has a good effect on teeth whitening. Detailed Implementation

[0033] To more clearly illustrate the present invention, the following description, in conjunction with preferred embodiments, further clarifies the invention. Those skilled in the art should understand that the specific descriptions below are illustrative rather than restrictive, and should not be construed as limiting the scope of protection of the present invention.

[0034] Unless otherwise stated, all percentages and ratios used herein are based on the total weight of the composition. Unless otherwise stated, all percentages, proportions, and contents of ingredients mentioned herein are based on the actual content of the ingredient and do not include solvents, fillers, or other substances that can be combined with these ingredients in commercially available products.

[0035] The term "includes / contains" in this article refers to other steps and components that may be added without affecting the final result.

[0036] The term "preferred" and its variations herein refer to embodiments of the invention that provide specific beneficial effects under particular conditions. However, other embodiments may also be preferred under the same or other conditions. Furthermore, the detailed description of one or more preferred embodiments does not imply that other embodiments are useless, nor is it intended to exclude other embodiments from the scope of the invention.

[0037] Unless otherwise specified in the embodiments of the present invention, the conditions shall be performed in accordance with conventional conditions or conditions recommended by the manufacturer; if the manufacturers of the reagents or instruments used are not specified, they are all conventional products that can be purchased commercially.

[0038] As one aspect of the present invention, an oral care composition comprising hyaluronic acid and polyphosphate is provided, comprising:

[0039] 1) Hyaluronic acid;

[0040] 2) Polyphosphates;

[0041] 3) Oral-acceptable carriers;

[0042] The polyphosphate is one or a combination of two or more of pyrophosphate, tripolyphosphate or hexametaphosphate;

[0043] The oral care composition has a pH value of 6-9.

[0044] The present invention unexpectedly discovered that the hyaluronic acid can improve the stability of polyphosphates in oral care compositions.

[0045] Hyaluronic acid

[0046] Hyaluronic acid, also known as glucuronic acid, is widely distributed throughout the human body. It is a high-molecular-weight polymer polysaccharide composed of D-glucuronic acid and N-acetylglucosamine units. The D-glucuronic acid and N-acetylglucosamine units are linked by β-1,3-glycosidic bonds, and the disaccharide units are linked by β-1,4-glycosidic bonds. The number of disaccharide units can reach as high as 25,000. Its molecular formula is (C... 14 H 21 NO 11 n has the following structure:

[0047]

[0048] This invention unexpectedly discovered that hyaluronic acid can enhance the stability of polyphosphates in oral care compositions, and at the same time enhance the teeth whitening effect of oral care compositions in preventing stains.

[0049] In some embodiments of the present invention, the hyaluronic acid refers to hyaluronic acid salt.

[0050] In some embodiments of the present invention, the hyaluronic acid salt refers to one or more of the sodium, potassium, and ammonium salts of hyaluronic acid.

[0051] In some embodiments of the present invention, the hyaluronic acid is present in the oral care composition at a weight percentage of 0.005-1%, for example, but not limited to 0.01-0.8%, 0.03-0.8%, 0.05-0.8%, 0.08-0.8%, 0.1-0.8%, 0.3-0.8%, 0.5-0.8%, 0.01-0.5%, 0.03-0.5%, 0.05-0.5%, 0.08-0.5%, 0.1-0.5%, 0.3-0.5%, 0.005-0.3%, 0.01-0.3%, 0.03-0.3%, 0.05-0.3%, 0.08-0.3%, 0.1-0.3%, 0.001-0.25%, 0.005-0.25%, 0 0.01-0.25%, 0.03-0.25%, 0.05-0.25%, 0.08-0.25%, 0.1-0.25%, 0.005-0.20%, 0.01-0.20%, 0.03-0.20%, 0.05-0.20%, 0.08-0.20%, 0.1-0.20%, 0.005-0.15%, 0.01-0.15%, 0.03-0.15%, 0.05-0.15%, 0.08-0.15%, 0.1-0.15%, 0.005-0.1%, 0.01-0.1%, 0.03-0.1%, 0.05-0.1%, 0.08-0.1%, 0.01-0.05%, or 0.03-0.05%.

[0052] Polyphosphate

[0053] The polyphosphates described in this invention are one or a combination of two or more of pyrophosphates, tripolyphosphates, or hexametaphosphates. They can be used in oral care compositions as pH adjusters, teeth whitening agents, or anti-tartar agents.

[0054] In some embodiments of the present invention, the polyphosphate is an acid salt of polyphosphate.

[0055] In some embodiments of the present invention, the polyphosphate is a potassium salt, sodium salt or ammonium salt of polyphosphate.

[0056] In some embodiments of the present invention, the polyphosphate is one or a combination of two or more of sodium pyrophosphate, trisodium monohydrogen pyrophosphate, disodium dihydrogen pyrophosphate, monosodium trihydrogen pyrophosphate, potassium pyrophosphate, potassium hydrogen pyrophosphate, dimethyl dihydrogen pyrophosphate, sodium tripolyphosphate, potassium tripolyphosphate, sodium hexametaphosphate, and potassium hexametaphosphate.

[0057] In some embodiments of the invention, the polyphosphate is present in the oral care composition at a weight percentage of 0.5-10%, for example, but not limited to 0.5-8%, 0.5-6%, 0.5-5%, 0.5-4.5%, 0.5-4%, 0.5-3.5%, 0.5-3%, 0.5-2.5%, 0.5-2%, 0.5-1.5%, 0.5-1%, 0.5-0.8%, 1-10%, 1-8%, 1-6%, 1-5%, 1-4%, 1-3.5%, 1-3%. 1-2.5%, 1-2%, 1-1.5%, 1-1.2%, 2-10%, 2-8%, 2-6%, 2-5%, 2-4.5%, 2-4%, 2-3.5%, 2-3%, 2-2.5%, 3%-10%, 3-8%, 3-6%, 3-5%, 3-4.5%, 3-4%, 3-3.5%, 4-10%, 4-8%, 4-6%, 4-5%, 5-10%, 5-8%, 5-6%, 6-10%, 6-8%, 6-10%, 8-10%.

[0058] Fluoride ion source

[0059] In some embodiments of the invention, the oral care composition further includes a fluoride ion source.

[0060] In some preferred embodiments of the present invention, the fluoride ion source is derived from one or more of stannous fluoride, sodium fluoride, potassium fluoride, amine fluoride, sodium monofluorophosphate, and zinc fluoride.

[0061] The present invention unexpectedly discovered that when an oral care composition contains both a fluoride ion source and polyphosphate, the addition of hyaluronic acid to the composition results in a higher retention rate of polyphosphate in the formulation.

[0062] In some embodiments of the present invention, the fluoride ions in the fluoride ion source account for 0.05-0.2% by weight in the oral care composition, for example, but not limited to, 0.08-0.2%, 0.1-0.2%, 0.15-0.2%, 0.05-0.15%, 0.08-0.15%, and 0.1-0.15%.

[0063] pH

[0064] The inventors unexpectedly discovered that maintaining the pH of an oral care composition containing polyphosphates and hyaluronic acid between 6 and 9 can significantly improve the stability of polyphosphates in the composition and inhibit their hydrolysis.

[0065] In some embodiments of the present invention, the pH range of the composition is 6-9, such as, but not limited to, 6.5-9, 7-9, 7.5-9, 8-9, 8.5-9, 6.5-9, 6-8.5, 6.5-8.5, 7-8.5, 7.5-8.5, 8-8.5, 6-8, 6.5-8, 7-8, 7.5-8, 6-7.5, 6.5-7.5, 7-7.5, 6-7, 6.5-7, 6-6.5.

[0066] In some embodiments of the present invention, the pH adjuster includes, but is not limited to, one or more of sodium hydroxide, hydrochloric acid, sulfuric acid, citric acid and its salts, lactic acid and its salts, phosphoric acid and its salts, phthalic acid and its salts, citric acid and its salts, and acetic acid and its salts.

[0067] Other oral acceptable carriers

[0068] In some embodiments of the present invention, the oral care composition is toothpaste, gel, oral care solution, liquid toothpaste, or tooth powder.

[0069] In this invention, "orally acceptable carrier" refers to any medium suitable for formulating the oral care compositions disclosed herein; an orally acceptable carrier is harmless to mammals when held in the mouth in the amount disclosed herein without being swallowed for a duration sufficient to allow effective contact with the tooth surface as required by the invention; generally, an orally acceptable carrier is not harmful even if unintentionally swallowed; suitable orally acceptable carriers include, for example, one or more of the following substances: water, abrasives, surfactants, thickeners, pH adjusters, humectants, flavorings, visual aids (e.g., pigments, dyes or mixtures thereof), anti-caries agents, antibacterial agents, whitening agents, desensitizing agents, preservatives, and mixtures thereof.

[0070] In some embodiments, the oral care composition includes toothpaste, gel, oral care solution, liquid toothpaste, or tooth powder.

[0071] As another aspect of the invention, the present invention provides an application of hyaluronic acid in oral care compositions to improve the stability of polyphosphates.

[0072] As another aspect of the invention, the present invention provides the application of an oral care composition comprising hyaluronic acid and polyphosphate in teeth whitening.

[0073] The method for calculating the growth rate of polyphosphate retention in this application

[0074] 1) Prepare a composition containing polyphosphate as a reference standard;

[0075] 2) Prepare a composition with the same polyphosphate weight ratio and added hyaluronic acid as a test sample;

[0076] 3) Detect the weight ratio of polyphosphate in the control and test samples after aging, i.e. the amount of polyphosphate retained in the composition;

[0077] 4) Calculate the growth rate of the retention:

[0078] Specific Implementation

[0080] Basic formula 1-2

[0081] Prepare basic formula 1 and basic formula 2 according to Table 1 below. All data in the table are weight percentages.

[0082] Table 1

[0083] Raw material name Basic Recipe 1 Basic Recipe 2 Hyaluronic acid / 0.05 Sodium pyrophosphate 1.0 1.0 Deionized water Add to 100% Add to 100%

[0084] As can be seen from Table 1:

[0085] Basic formulation 1 is a 1% sodium pyrophosphate aqueous solution by weight;

[0086] Basic formulation 2 is a mixed aqueous solution of sodium pyrophosphate at a weight ratio of 1% and hyaluronic acid at a weight ratio of 0.05%.

[0087] Example 1, Comparative Example 1

[0088] Comparative Example 1: The pH of Basic Formula 1 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 1.

[0089] Example 1: The pH value of the basic formula 2 was adjusted to 7 with hydrochloric acid to obtain Example 1.

[0090] Example 1 and Comparative Example 1 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 1 was used as a control, and the growth rate of pyrophosphate retention in Example 1 was calculated. The results are shown in Table 2.

[0091] Table 2:

[0092] Example 1 growth rate of retention 31.82％ pH value 7

[0093] As can be seen from Table 2:

[0094] When 0.05% hyaluronic acid was added to a 1% (by weight) sodium pyrophosphate solution and the pH was adjusted to 7, the pyrophosphate retention increased by 31.82%. This means that, under pH 7 conditions, hyaluronic acid significantly improved the stability of pyrophosphate compared to the control group.

[0095] Comparative Examples 2-3

[0096] Comparative Example 2: The pH of Basic Formula 1 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 2.

[0097] Comparative Example 3: The pH of Basic Formula 2 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 3.

[0098] Comparative Examples 2 and 3 were aged at 40℃ for 3 months, and the weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 2 was used as a control, and the growth rate of pyrophosphate retention in Comparative Example 3 was calculated. The results are shown in Table 3.

[0099] Table 3:

[0100] Comparative Example 3 growth rate of retention -2.66％ pH value 5.5

[0101] As can be seen from Table 3:

[0102] When 0.05% hyaluronic acid was added to a 1% sodium pyrophosphate solution and the pH was adjusted to 5.5, the growth rate of pyrophosphate retention was -2.66%. This means that under the condition of pH adjustment to 5.5, compared with the control group, hyaluronic acid did not show a stabilizing effect on pyrophosphate, but instead accelerated the hydrolysis of pyrophosphate.

[0103] Example 2, Comparative Example 4

[0104] Comparative Example 4: The pH of Basic Formula 1 was adjusted to 6 with hydrochloric acid to obtain Comparative Example 4.

[0105] Example 2: The pH value of the basic formula 2 was adjusted to 6 with hydrochloric acid to obtain Example 2.

[0106] Example 2 and Comparative Example 4 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 4 was used as a control, and the growth rate of pyrophosphate retention in Example 2 was calculated. The results are shown in Table 4.

[0107] Table 4:

[0108] Example 2 growth rate of retention 1.73％ pH value 6

[0109] As can be seen from Table 4:

[0110] When 0.05% hyaluronic acid was added to a 1% sodium pyrophosphate solution and the pH was adjusted to 6, the pyrophosphate retention increased by 1.73%, which was an increase, but the increase was small. That is, under the condition of pH adjustment to 6, compared with the control example 4, hyaluronic acid can improve the stability of pyrophosphate, but the increase is not significant.

[0111] Example 3, Comparative Example 5

[0112] Comparative Example 5: The pH of Basic Formula 1 was adjusted to 6.5 with hydrochloric acid to obtain Comparative Example 5.

[0113] Example 3: The pH value of the basic formula 2 was adjusted to 6.5 with hydrochloric acid to obtain Example 3.

[0114] Example 3 and Comparative Example 5 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 5 was used as a control, and the growth rate of pyrophosphate retention in Example 3 was calculated. The results are shown in Table 5.

[0115] Table 5:

[0116] Example 3 growth rate of retention 19.65％ pH value 6.5

[0117] As can be seen from Table 5:

[0118] When 0.05% hyaluronic acid is added to a 1% sodium pyrophosphate solution and the pH is adjusted to 6.5, the pyrophosphate retention rate increases by 19.65%, indicating that hyaluronic acid can significantly improve the stability of pyrophosphate under pH adjustment to 6.5.

[0119] Example 4, Comparative Example 6

[0120] Comparative Example 6: The pH of Basic Formula 1 was adjusted to 7.5 with hydrochloric acid to obtain Comparative Example 6.

[0121] Example 4: The pH value of the basic formula 2 was adjusted to 7.5 with hydrochloric acid to obtain Example 4.

[0122] Example 4 and Comparative Example 6 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 6 was used as a control, and the growth rate of pyrophosphate retention in Example 4 was calculated. The results are shown in Table 6.

[0123] Table 6:

[0124] Example 4 growth rate of retention 32.36％ pH value 7.5

[0125] As can be seen from Table 6:

[0126] When 0.05% hyaluronic acid was added to a 1% by weight sodium pyrophosphate solution and the pH was adjusted to 7.5, the pyrophosphate retention increased by 32.36%; that is, under the condition of pH adjustment to 7.5, hyaluronic acid can significantly improve the stability of pyrophosphate.

[0127] Example 5, Comparative Example 7

[0128] Comparative Example 7: The pH of Basic Formula 1 was adjusted to 8 with hydrochloric acid to obtain Comparative Example 7.

[0129] Example 5: The pH value of the basic formula 2 was adjusted to 8 with hydrochloric acid to obtain Example 5.

[0130] Example 5 and Comparative Example 7 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 7 was used as a control, and the growth rate of pyrophosphate retention in Example 5 was calculated. The results are shown in Table 7.

[0131] Table 7:

[0132] Example 5 growth rate of retention 25.79％ pH value 8

[0133] As shown in Table 7, when 0.05% hyaluronic acid was added to a 1% sodium pyrophosphate solution and the pH was adjusted to 8, the pyrophosphate retention increased by 25.79%, indicating that hyaluronic acid can significantly improve the stability of pyrophosphate under pH adjustment to 8.

[0134] Example 6, Comparative Example 8

[0135] Comparative Example 8: The pH of Basic Formula 1 was adjusted to 8.5 with hydrochloric acid to obtain Comparative Example 8.

[0136] Example 6: The pH value of the basic formula 2 was adjusted to 8.5 with hydrochloric acid to obtain Example 6.

[0137] Example 6 and Comparative Example 8 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 8 was used as a control, and the growth rate of pyrophosphate retention in Example 6 was calculated. The results are shown in Table 8.

[0138] Table 8:

[0139] Example 6 growth rate of retention 11.49％ pH value 8.5

[0140] As can be seen from Table 11, when 0.05% hyaluronic acid is added to a 1% sodium pyrophosphate solution and the pH is adjusted to 8.5, the growth rate of pyrophosphate retention is 11.49%, which means that hyaluronic acid can still significantly improve the stability of pyrophosphate under the condition of pH adjustment to 8.5.

[0141] Example 7, Comparative Example 9

[0142] Comparative Example 9: The pH of Basic Formula 1 was adjusted to 9 with hydrochloric acid to obtain Comparative Example 9.

[0143] Example 7: The pH of the basic formula 2 was adjusted to 9 with hydrochloric acid to obtain Example 7.

[0144] Example 7 and Comparative Example 9 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 9 was used as a control, and the growth rate of pyrophosphate retention in Example 7 was calculated. The results are shown in Table 9.

[0145] Table 9:

[0146] Example 7 growth rate of retention 1.06％ pH value 9

[0147] As can be seen from Table 9:

[0148] When 0.05% hyaluronic acid is added to a 1% sodium pyrophosphate solution and the pH is adjusted to 9, the pyrophosphate retention rate increases by 1.06%. This means that even at pH 9, hyaluronic acid can still improve the stability of pyrophosphate, but the improvement is limited.

[0149] As shown in Tables 1-9 above, adding 0.05% hyaluronic acid to a 1% sodium pyrophosphate solution can improve the stability of pyrophosphate when the pH is 6-9; and can significantly improve the stability of pyrophosphate when the pH is 6.5-8.5.

[0150] Basic formula 3-4

[0151] Prepare basic formula 3 and basic formula 4 according to Table 10. All data in the table are weight percentages.

[0152] Table 10

[0153] Raw material name Basic Formula 3 Basic Recipe 4 Hyaluronic acid / 0.01 Sodium pyrophosphate 0.5 0.5 Deionized water Add to 100% Add to 100%

[0154] As can be seen from Table 10:

[0155] Basic formulation 3 is a 0.5% sodium pyrophosphate aqueous solution by weight;

[0156] Basic formulation 4 is a mixed aqueous solution of sodium pyrophosphate at a weight ratio of 0.5% and hyaluronic acid at a weight ratio of 0.01%.

[0157] Example 8, Comparative Example 10

[0158] Comparative Example 10: The pH of Basic Formula 3 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 10.

[0159] Example 8: The pH of the basic formula 4 was adjusted to 7 with hydrochloric acid to obtain Example 8.

[0160] Example 8 and Comparative Example 10 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 10 was used as a control, and the growth rate of pyrophosphate retention in Example 8 was calculated. The results are shown in Table 11.

[0161] Table 11:

[0162] Example 8 growth rate of retention 36.53％ pH value 7

[0163] As can be seen from Table 11:

[0164] When 0.01% hyaluronic acid was added to a 0.5% sodium pyrophosphate solution and the pH was adjusted to 7, the pyrophosphate retention increased by 36.53%. This means that under pH adjustment to 7, 0.01% hyaluronic acid significantly improved the stability of 0.5% sodium pyrophosphate.

[0165] Basic formula 5-6

[0166] Prepare basic formula 5 and basic formula 6 according to Table 12. All data in the table are weight percentages.

[0167] Table 12

[0168] Raw material name Basic Recipe 5 Basic Formula 6 Hyaluronic acid / 0.5 Sodium pyrophosphate 10 10 Deionized water Add to 100% Add to 100%

[0169] As can be seen from Table 12:

[0170] Basic formulation 5 is a 10% (by weight) sodium pyrophosphate aqueous solution;

[0171] Basic formulation 6 is a mixed aqueous solution of sodium pyrophosphate at a weight ratio of 10% and hyaluronic acid at a weight ratio of 0.5%.

[0172] Example 9, Comparative Example 11

[0173] Comparative Example 11: The pH of Basic Formula 5 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 11.

[0174] Example 9: The pH of the basic formula 6 was adjusted to 7 with hydrochloric acid to obtain Example 9.

[0175] Example 9 and Comparative Example 11 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 11 was used as a control, and the growth rate of pyrophosphate retention in Example 9 was calculated. The results are shown in Table 13.

[0176] Table 13:

[0177] Example 9 growth rate of retention 25.63％ pH value 7

[0178] As can be seen from Table 13:

[0179] When 0.5% hyaluronic acid was added to a 10% sodium pyrophosphate solution and the pH was adjusted to 7, the pyrophosphate retention increased by 25.63%. This means that under pH 7 conditions, 0.5% hyaluronic acid also significantly improved the stability of 10% sodium pyrophosphate.

[0180] Basic formula 7-8

[0181] Prepare basic formula 7 and basic formula 8 according to Table 14. All data in the table are weight percentages.

[0182] Table 14

[0183] Raw material name Basic Formula 7 Basic Formula 8 Hyaluronic acid / 0.05 Sodium tripolyphosphate 1.0 1.0 Deionized water Add to 100% Add to 100%

[0184] As can be seen from Table 14:

[0185] Basic formula 7 is a 1% sodium tripolyphosphate aqueous solution by weight;

[0186] The basic formula 8 is a mixed aqueous solution of sodium tripolyphosphate at a weight ratio of 1% and hyaluronic acid at a weight ratio of 0.05%.

[0187] Comparative Examples 12-13

[0188] Comparative Example 12: The pH of the basic formula 7 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 12.

[0189] Comparative Example 13: The pH of the basic formula 8 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 13.

[0190] Comparative Examples 12 and 13 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 12 was used as a control, and the growth rate of tripolyphosphate retention in Comparative Example 13 was calculated. The results are shown in Table 15.

[0191] Example 10, Comparative Example 14

[0192] Comparative Example 14: The pH of the basic formula 7 was adjusted to 6 with hydrochloric acid to obtain Comparative Example 14.

[0193] Example 10: The pH of the basic formula 8 was adjusted to 6 with hydrochloric acid to obtain Example 10.

[0194] Comparative Example 14 and Example 10 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 14 was used as a control, and the growth rate of tripolyphosphate retention in Example 10 was calculated. The results are shown in Table 15.

[0195] Example 11, Comparative Example 15

[0196] Comparative Example 15: The pH of Basic Formula 7 was adjusted to 6.5 with hydrochloric acid to obtain Comparative Example 15.

[0197] Example 11: The pH value of the basic formula 8 was adjusted to 6.5 with hydrochloric acid to obtain Example 11.

[0198] Comparative Example 15 and Example 11 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 15 was used as a control, and the growth rate of tripolyphosphate retention in Example 11 was calculated. The results are shown in Table 15.

[0199] Example 12, Comparative Example 16

[0200] Comparative Example 16: The pH of the basic formula 7 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 16.

[0201] Example 12: The pH of the basic formula 8 was adjusted to 7 with hydrochloric acid to obtain Example 12.

[0202] Comparative Example 16 and Example 12 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 16 was used as a control, and the growth rate of tripolyphosphate retention in Example 12 was calculated. The results are shown in Table 15.

[0203] Example 13, Comparative Example 17

[0204] Comparative Example 17: The pH of the basic formula 7 was adjusted to 8 with hydrochloric acid to obtain Comparative Example 17.

[0205] Example 13: The pH value of the basic formula 8 was adjusted to 8 with hydrochloric acid to obtain Example 13.

[0206] Comparative Example 17 and Example 13 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 17 was used as a control, and the growth rate of tripolyphosphate retention in Example 13 was calculated. The results are shown in Table 15.

[0207] Example 14, Comparative Example 18

[0208] Comparative Example 18: The pH of the basic formula 7 was adjusted to 8.5 with hydrochloric acid to obtain Comparative Example 18.

[0209] Example 14: The pH of the basic formula 8 was adjusted to 8.5 with hydrochloric acid to obtain Example 14.

[0210] Comparative Example 18 and Example 14 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 18 was used as a control, and the growth rate of tripolyphosphate retention in Example 14 was calculated. The results are shown in Table 15.

[0211] Example 15, Comparative Example 19

[0212] Comparative Example 19: The pH of the basic formula 7 was adjusted to 9 with hydrochloric acid to obtain Comparative Example 19.

[0213] Example 15: The pH of the basic formula 8 was adjusted to 9 with hydrochloric acid to obtain Example 15.

[0214] Comparative Example 19 and Example 15 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 19 was used as a control, and the growth rate of tripolyphosphate retention in Example 15 was calculated. The results are shown in Table 15.

[0215] Table 15: Growth rate of tripolyphosphate retention in Examples 10-15 and Comparative Example 13

[0216] Comparative Example 13 Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 pH value 5.5 6 6.5 7 8 8.5 9 growth rate of retention 1.89 7.65 25.28 35.55 26.73 15.21 4.97

[0217] Table 15 shows that:

[0218] Hyaluronic acid can improve the stability of tripolyphosphate within the pH range of 5.5-9; the improvement is more significant, especially at pH 6.5-8.5.

[0219] Basic formula 9-10

[0220] Prepare basic formula 9 and basic formula 10 according to Table 16. All data in the table are weight percentages.

[0221] Table 16

[0222] Raw material name Basic Formula 9 Basic recipe 10 Hyaluronic acid / 0.01 Sodium tripolyphosphate 0.5 0.5 Deionized water Add to 100% Add to 100%

[0223] As can be seen from Table 16:

[0224] Basic formula 9 is a 0.5% sodium tripolyphosphate aqueous solution.

[0225] The basic formulation 10 is a mixed aqueous solution of 0.5% sodium tripolyphosphate and 0.01% hyaluronic acid.

[0226] Example 16, Comparative Example 20

[0227] Comparative Example 20: The pH of Basic Formula 9 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 20.

[0228] Example 16: The pH of the basic formula 10 was adjusted to 7 with hydrochloric acid to obtain Example 16.

[0229] Example 16 and Comparative Example 20 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 20 was used as a control, and the growth rate of tripolyphosphate retention in Example 16 was calculated. The results are shown in Table 17.

[0230] Table 17:

[0231] Example 16 growth rate of retention 40.47％ pH value 7

[0232] As can be seen from Table 17:

[0233] When 0.01% hyaluronic acid was added to a 0.5% sodium tripolyphosphate solution and the pH was adjusted to 7, the growth rate of tripolyphosphate retention was 40.47%; that is, under the condition of pH adjustment to 7, 0.01% hyaluronic acid also significantly improved the stability of 0.5% sodium tripolyphosphate.

[0234] Basic formula 11-12

[0235] Prepare basic formula 11 and basic formula 12 according to Table 18. All data in the table are weight percentages.

[0236] Table 18

[0237] Raw material name Basic Recipe 11 Basic recipe 12 Hyaluronic acid / 0.5 Sodium tripolyphosphate 10 10 Deionized water Add to 100% Add to 100%

[0238] As can be seen from Table 18:

[0239] Basic formulation 11 is a 10% sodium tripolyphosphate aqueous solution;

[0240] The basic formula 12 is a mixed aqueous solution of 10% sodium tripolyphosphate and 0.5% hyaluronic acid.

[0241] Example 17, Comparative Example 21

[0242] Comparative Example 21: The pH of the basic formula 11 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 21.

[0243] Example 17: The pH of the basic formula 12 was adjusted to 7 with hydrochloric acid to obtain Example 17.

[0244] Example 17 and Comparative Example 21 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 21 was used as a control, and the growth rate of tripolyphosphate retention in Example 17 was calculated. The results are shown in Table 19.

[0245] Table 19:

[0246] Example 17 growth rate of retention 27.52％ pH value 7

[0247] As can be seen from Table 19:

[0248] When 0.5% hyaluronic acid was added to a 10% sodium tripolyphosphate solution and the pH was adjusted to 7, the growth rate of tripolyphosphate retention was 27.52%; that is, under the condition of pH adjustment to 7, 0.5% hyaluronic acid also significantly improved the stability of 10% sodium tripolyphosphate.

[0249] Basic recipe 13-14

[0250] Prepare basic formula 13 and basic formula 14 according to Table 20. All data in the table are weight percentages.

[0251] Table 20

[0252] Raw material name Basic Recipe 13 Basic Recipe 14 Hyaluronic acid / 0.05 Sodium hexametaphosphate 1.0 1.0 Deionized water Add to 100% Add to 100%

[0253] As can be seen from Table 20:

[0254] Basic formula 13 is an aqueous solution of sodium hexametaphosphate at a weight ratio of 1%.

[0255] Basic Formula 14 is based on Basic Formula 13 with the addition of 0.05% hyaluronic acid by weight.

[0256] Comparative Examples 22-23

[0257] Comparative Example 22: The pH of Basic Formula 13 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 22.

[0258] Comparative Example 23: The pH of Basic Formula 14 was adjusted to 5.5 with hydrochloric acid to obtain Comparative Example 23.

[0259] Comparative Examples 22 and 23 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 22 was used as a control, and the growth rate of hexametaphosphate retention in Comparative Example 23 was calculated. The results are shown in Table 21.

[0260] Example 18, Comparative Example 24

[0261] Comparative Example 24: The pH of Basic Formula 13 was adjusted to 6 with hydrochloric acid to obtain Comparative Example 24.

[0262] Example 18: The pH of the basic formula 14 was adjusted to 6 with hydrochloric acid to obtain Example 18.

[0263] Comparative Example 24 and Example 18 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 24 was used as a control, and the growth rate of hexametaphosphate retention in Example 18 was calculated. The results are shown in Table 21.

[0264] Example 19, Comparative Example 25

[0265] Comparative Example 25: The pH of Basic Formula 13 was adjusted to 6.5 with hydrochloric acid to obtain Comparative Example 25.

[0266] Example 19: The pH of the basic formula 14 was adjusted to 6.5 with hydrochloric acid to obtain Example 19.

[0267] Comparative Example 25 and Example 19 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 25 was used as a control, and the growth rate of hexametaphosphate retention in Example 19 was calculated. The results are shown in Table 21.

[0268] Example 20, Comparative Example 26

[0269] Comparative Example 26: The pH of Basic Formula 13 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 26.

[0270] Example 20: The pH of the basic formula 14 was adjusted to 7 with hydrochloric acid to obtain Example 20.

[0271] Comparative Example 26 and Example 20 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 26 was used as a control, and the growth rate of hexametaphosphate retention in Example 20 was calculated. The results are shown in Table 21.

[0272] Example 21, Comparative Example 27

[0273] Comparative Example 27: The pH of Basic Formula 13 was adjusted to 8 with hydrochloric acid to obtain Comparative Example 27.

[0274] Example 21: The pH value of the basic formula 14 was adjusted to 8 with hydrochloric acid to obtain Example 21.

[0275] Comparative Example 27 and Example 21 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 27 was used as a control, and the growth rate of hexametaphosphate retention in Example 21 was calculated. The results are shown in Table 21.

[0276] Example 22, Comparative Example 28

[0277] Comparative Example 28: The pH of Basic Formula 13 was adjusted to 8.5 with hydrochloric acid to obtain Comparative Example 28.

[0278] Example 22: The pH value of the basic formula 14 was adjusted to 8.5 with hydrochloric acid to obtain Example 22.

[0279] Comparative Example 28 and Example 22 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 28 was used as a control, and the growth rate of hexametaphosphate retention in Example 22 was calculated. The results are shown in Table 21.

[0280] Example 23, Comparative Example 29

[0281] Comparative Example 29: The pH of Basic Formula 13 was adjusted to 9 with hydrochloric acid to obtain Comparative Example 29.

[0282] Example 23: The pH of the basic formula 14 was adjusted to 9 with hydrochloric acid to obtain Example 23.

[0283] Comparative Example 29 and Example 23 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 29 was used as a control, and the growth rate of hexametaphosphate retention in Example 23 was calculated. The results are shown in Table 21.

[0284] Table 21: Growth rate of hexametaphosphate retention in Examples 18-23 and Comparative Example 23

[0285] Comparative Example 23 Example 18 Example 19 Example 20 Example 21 Example 22 Example 23 pH value 5.5 6 6.5 7 8 8.5 9 growth rate of retention 2.77 8.57 27.67 39.18 27.98 16.63 3.46

[0286] As can be seen from Table 21:

[0287] Hyaluronic acid can improve the stability of hexametaphosphate in the pH range of 5.5-9, especially in the pH range of 6.5-8.5, where the improvement is more significant.

[0288] Basic formula 15-16

[0289] Prepare basic formula 15 and basic formula 16 according to Table 22. All data in the table are weight percentages.

[0290] Table 22

[0291] Raw material name Basic recipe 15 Basic Recipe 16 Hyaluronic acid / 0.01 Sodium hexametaphosphate 0.5 0.5 Deionized water Add to 100% Add to 100%

[0292] As can be seen from Table 22:

[0293] The basic formula 15 is a 0.5% sodium hexametaphosphate aqueous solution;

[0294] The basic formula 16 is a mixed aqueous solution of 0.5% sodium hexametaphosphate and 0.01% hyaluronic acid.

[0295] Example 24, Comparative Example 30

[0296] Comparative Example 30: The pH of Basic Formula 15 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 30.

[0297] Example 24: The pH of the basic formula 16 was adjusted to 7 with hydrochloric acid to obtain Example 24.

[0298] Example 24 and Comparative Example 30 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 30 was used as a control, and the growth rate of hexametaphosphate retention in Example 24 was calculated. The results are shown in Table 23.

[0299] Table 23:

[0300] Example 24 growth rate of retention 45.53％ pH value 7

[0301] As can be seen from Table 23:

[0302] When 0.01% hyaluronic acid is added to a 0.5% sodium hexametaphosphate aqueous solution and the pH is adjusted to 7, the hexametaphosphate retention rate increases by 45.53%. This means that under pH adjustment to 7, 0.01% hyaluronic acid by weight also significantly improves the stability of 0.5% hexametaphosphate by weight.

[0303] Basic formula 17-18

[0304] Prepare basic formula 17 and basic formula 18 according to Table 24. All data in the table are weight percentages.

[0305] Table 24

[0306] Raw material name Basic Recipe 17 Basic Recipe 18 Hyaluronic acid / 0.5 Sodium hexametaphosphate 10 10 Deionized water Add to 100% Add to 100%

[0307] As can be seen from Table 24:

[0308] The basic formula 17 is a 10% sodium hexametaphosphate aqueous solution;

[0309] The basic formula 18 is a mixed aqueous solution of 10% sodium hexametaphosphate and 0.5% hyaluronic acid.

[0310] Example 25, Comparative Example 31

[0311] Comparative Example 31: The pH of Basic Formula 17 was adjusted to 7 using hydrochloric acid to obtain Comparative Example 31.

[0312] Example 25: The pH of the basic formula 18 was adjusted to 7 with hydrochloric acid to obtain Example 25.

[0313] Example 25 and Comparative Example 31 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 31 was used as a control, and the growth rate of hexametaphosphate retention in Example 25 was calculated. The results are shown in Table 25.

[0314] Table 25:

[0315] Example 25 growth rate of retention 26.66％ pH value 7

[0316] As can be seen from Table 25:

[0317] When 0.5% hyaluronic acid is added to a 10% sodium hexametaphosphate aqueous solution and the pH is adjusted to 7, the hexametaphosphate retention rate increases by 26.66%; that is, under the condition of pH adjustment to 7, 0.5% by weight hyaluronic acid also has a significant effect on improving the stability of 10% by weight hexametaphosphate.

[0318] Basic formula 19-20

[0319] Prepare basic formula 19 and basic formula 20 according to Table 26. All data in the table are weight percentages.

[0320] Table 26

[0321] Raw material name Basic Recipe 19 Basic recipe 20 Hyaluronic acid / 0.05 Sodium pyrophosphate 1.0 1.0 Sodium fluoride 0.22 0.22 Deionized water Add to 100% Add to 100%

[0322] As can be seen from Table 26:

[0323] Basic formulation 19 is a mixed aqueous solution of 1.0% sodium pyrophosphate and 0.22% sodium fluoride;

[0324] Basic Formula 20 is based on Basic Formula 19 with the addition of 0.05% hyaluronic acid.

[0325] Example 26, Comparative Example 32

[0326] Comparative Example 32: The pH of Basic Formula 19 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 32.

[0327] Example 26: The pH of the basic formula 20 was adjusted to 7 with hydrochloric acid to obtain Example 26.

[0328] Example 26 and Comparative Example 32 were aged at 40°C for 3 months. The weight ratio of pyrophosphate retained in the formulation was measured. Comparative Example 32 was used as a control, and the growth rate of pyrophosphate retention in Example 26 was calculated. The results are shown in Table 27. For ease of comparison, the growth rate data of retention in Example 1 are included in Table 27.

[0329] Table 27:

[0330] Example 1 Example 26 growth rate of retention 31.82％ 40.32％ pH value 7 7

[0331] As can be seen from Table 27:

[0332] When hyaluronic acid was added to a solution containing 1.0% sodium pyrophosphate and 0.22% sodium fluoride, and the pH was adjusted to 7, the growth rate of pyrophosphate retention was 40.32%. That is, under the same pH conditions, the growth rate of retention in Example 25 containing sodium fluoride was better than that in Example 1.

[0333] Basic recipe 21-22

[0334] Prepare basic formula 21 and basic formula 22 according to Table 28. All data in the table are weight percentages.

[0335] Table 28

[0336] Raw material name Basic Recipe 21 Basic Recipe 22 Hyaluronic acid / 0.05 Sodium tripolyphosphate 1.0 1.0 Sodium fluoride 0.22 0.22 Deionized water Add to 100% Add to 100%

[0337] As can be seen from Table 28:

[0338] Basic formulation 21 is a mixed aqueous solution containing 1.0% sodium tripolyphosphate and 0.22% sodium fluoride by weight.

[0339] Basic Formula 22 is based on Basic Formula 21 with the addition of 0.05% hyaluronic acid by weight.

[0340] Example 27, Comparative Example 33

[0341] Comparative Example 33: The pH of Basic Formula 21 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 33.

[0342] Example 27: The pH of the basic formula 22 was adjusted to 7 with hydrochloric acid to obtain Example 27.

[0343] Example 27 and Comparative Example 33 were aged at 40°C for 3 months. The weight ratio of tripolyphosphate retained in the formulation was measured. Comparative Example 33 was used as a control, and the growth rate of tripolyphosphate retention in Example 27 was calculated. The results are shown in Table 29. For ease of comparison, the growth rate of retention in Example 12 is also shown in Table 29.

[0344] Table 29:

[0345] Example 12 Example 27 growth rate of retention 35.55％ 43.93％ pH 7 7

[0346] As can be seen from Table 29:

[0347] When hyaluronic acid was added to a solution containing 1.0% sodium tripolyphosphate and 0.22% sodium fluoride, and the pH was adjusted to 7, the growth rate of tripolyphosphate retention was 43.93%; that is, under the same pH conditions, the growth rate of retention in Example 27 containing sodium fluoride was better than that in Example 12.

[0348] Basic recipe 23-24

[0349] Prepare basic formula 23 and basic formula 24 according to Table 30. All data in the table are weight percentages.

[0350] Table 30

[0351] Raw material name Basic Recipe 23 Basic Recipe 24 Hyaluronic acid / 0.05 Sodium hexametaphosphate 1.0 1.0 Sodium fluoride 0.22 0.22 Deionized water Add to 100% Add to 100%

[0352] As can be seen from Table 30:

[0353] The basic formulation 23 is a mixed aqueous solution of 1.0% sodium hexametaphosphate and 0.22% sodium fluoride by weight.

[0354] Basic Formula 24 is based on Basic Formula 23 with the addition of 0.05% hyaluronic acid by weight.

[0355] Example 28, Comparative Example 34

[0356] Comparative Example 34: The pH of Basic Formula 23 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 34.

[0357] Example 28: The pH of the basic formula 24 was adjusted to 7 with hydrochloric acid to obtain Example 28.

[0358] Example 28 and Comparative Example 34 were aged at 40°C for 3 months. The weight ratio of hexametaphosphate retained in the formulation was measured. Comparative Example 34 was used as a control, and the growth rate of hexametaphosphate retention in Example 28 was calculated. The results are shown in Table 31. For ease of comparison, the growth rate of retention in Example 20 is also included in Table 31.

[0359] Table 31:

[0360] Example 20 Example 28 growth rate of retention 39.18％ 46.88％ pH value 7 7

[0361] As can be seen from Table 31:

[0362] When 0.05% hyaluronic acid was added to a mixed aqueous solution containing 1.0% sodium hexametaphosphate and 0.22% sodium fluoride, and the pH was adjusted to 7, the growth rate of hexametaphosphate retention was 46.88%; that is, under the same pH conditions, the growth rate of retention in Example 28 containing sodium fluoride was better than that in Example 20.

[0363] Methods for evaluating the effectiveness of preventing exogenous pigmentation:

[0364] 1) For each set of examples and comparative examples, select ten HAp [hydroxyapatite tablets], soak them in sterile saliva for 3 hours, take them out, rinse them in 10ml of deionized water for 10s, and measure the L value with a colorimeter;

[0365] 2) Take 10 ml of the solution sample from the example or comparative example, immerse the above HAp tablets in the solution for 3 min, remove them, and rinse them in 10 ml of deionized water for 10 s;

[0366] 3) Soak each group of HAP tablets in 15ml of black tea solution for 3 minutes, remove them, rinse them in 10ml of deionized water for 10 seconds, and measure the L value of the HAP tablet surface with a colorimeter.

[0367] 4) Calculate the change in L value before and after each group of HAp tablets, i.e., L 前 -L 后 The average value was calculated, and the smaller the change in the L value, the better the ability to prevent pigmentation.

[0368] The formulations of Comparative Example 1, Example 1, Comparative Example 16, Example 12, Comparative Example 26 and Example 20 were tested for their effectiveness in preventing exogenous pigmentation. The results are shown in Table 32 below.

[0369] Table 32:

[0370] Comparative Example 1 Example 1 Comparative Example 15 Example 11 Comparative Example 25 Example 19 ΔL 5.67 5.06 5.11 4.53 4.94 4.34

[0371] As can be seen from Table 32:

[0372] Compared to the corresponding comparative example, the example with added hyaluronic acid showed better pigmentation prevention effect.

[0373] Basic formula 25-30

[0374] Prepare the basic formula 25-30 according to Table 33. All data in the table are weight percentages.

[0375] Table 33:

[0376]

[0377] As can be seen from Table 33:

[0378] The basic formula 25 is a 5% sodium pyrophosphate aqueous solution;

[0379] The basic formula 26 is a mixed aqueous solution of 5% sodium pyrophosphate and 0.5% hyaluronic acid.

[0380] The basic formula 27 is a 5% sodium tripolyphosphate aqueous solution;

[0381] The basic formula 28 is a mixed aqueous solution of 5% sodium tripolyphosphate and 0.5% hyaluronic acid.

[0382] The basic formula 29 is a 5% sodium hexametaphosphate aqueous solution;

[0383] The basic formula 30 is a mixed aqueous solution of 5% sodium hexametaphosphate and 0.5% hyaluronic acid.

[0384] Comparative Examples 35-37, Examples 29-31

[0385] Comparative Example 35: The pH of Basic Formula 25 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 35.

[0386] Example 29: The pH of the basic formula 26 was adjusted to 7 with hydrochloric acid to obtain Example 29.

[0387] Comparative Example 36: The pH of the basic formula 27 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 36.

[0388] Example 30: The pH of the basic formula 28 was adjusted to 7 with hydrochloric acid to obtain Example 30.

[0389] Comparative Example 37: The pH of Basic Formula 29 was adjusted to 7 with hydrochloric acid to obtain Comparative Example 37.

[0390] Example 31: The pH value of the basic formula 30 was adjusted to 7 with hydrochloric acid to obtain Example 31.

[0391] Comparative Examples 35-37 and Examples 29-31 were aged at 40°C for 3 months, and the weight ratio of polyphosphate retained in the formulation was measured. The growth rate of pyrophosphate retention in Example 29 was calculated using Comparative Example 35 as a control; the growth rate of tripolyphosphate retention in Example 30 was calculated using Comparative Example 36 as a control; and the growth rate of pyrophosphate retention in Example 31 was calculated using Comparative Example 37 as a control. The results of the above retention growth rates are shown in Table 34.

[0392] Table 34:

[0393] Example 29 Example 30 Example 31 growth rate of retention 29.41％ 32.65％ 30.59 pH value 7 7 7

[0394] The formulations of Comparative Examples 35-37 and Examples 29-31 were tested for their effectiveness in preventing exogenous pigmentation. The results are shown in Table 35 below.

[0395] Table 35:

[0396] Comparative Example 35 Example 29 Comparative Example 36 Example 30 Comparative Example 37 Example 31 ΔL 3.51 3.07 3.13 2.76 2.54 2.21

[0397] As can be seen from Table 35:

[0398] Compared to the corresponding comparative example, the example with added hyaluronic acid showed better pigmentation prevention effect.

[0399] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make other variations or modifications based on the above description. It is impossible to exhaustively list all embodiments here. All obvious variations or modifications derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

1. An application of hyaluronic acid in improving the stability of polyphosphates in oral care compositions, characterized in that, The oral care composition comprises: 1) hyaluronic acid; 2) polyphosphate; 3) an orally acceptable carrier; wherein the polyphosphate is one or a combination of two or more of pyrophosphate, triphosphate or hexametaphosphate; The pH value of the oral care composition is 6.5-8.5; The polyphosphate accounts for 0.5-10% by weight in the oral care composition; The hyaluronic acid accounts for 0.005-1% of the mass of the oral care composition.

2. The application according to claim 1, characterized in that: The polyphosphate is an acid salt of polyphosphate.

3. The application according to any one of claims 1-2, characterized in that: The polyphosphate is one or a combination of two or more of the potassium, ammonium, or sodium salts of polyphosphate.

4. The application according to claim 1, characterized in that: The polyphosphate accounts for 1.0%-5.0% of the weight of the oral care composition.

5. The application according to claim 1, characterized in that: The hyaluronic acid is one or a combination of two or more of the sodium, potassium, and ammonium salts of hyaluronic acid.

6. The application according to claim 1, characterized in that: The hyaluronic acid constitutes 0.01% - 0.5% of the oral care composition by mass.

7. The application according to claim 6, characterized in that: The hyaluronic acid constitutes 0.05%-0.2% of the oral care composition by mass.

8. The application according to claim 1, characterized in that: The oral care composition also includes a fluoride ion source.

9. The application according to claim 8, characterized in that: The fluoride ion source is one or more of stannous fluoride, sodium fluoride, potassium fluoride, amine fluoride, sodium monofluorophosphate, and zinc fluoride.

10. The application according to claim 9, characterized in that: The fluoride ions in the fluoride ion source account for 0.05-0.2% of the weight of the oral care composition.

11. The application according to claim 1, characterized in that: The oral care composition includes toothpaste, gel, oral care solution, or tooth powder.

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