梨Pybbx24基因突变及其矮化功能的应用

By cloning the Pybbx24 gene from pear trees and overexpressing it in Arabidopsis thaliana and tobacco, we verified its function in promoting plant dwarfing, which solved the problem of insufficient gene resources for pear tree dwarfing breeding, achieved the effect of fruit tree dwarfing breeding, and reduced agricultural costs.

CN117402892BActive Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2023-10-09
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The existing technology has limited understanding of the molecular mechanisms of pear tree dwarfing, and lacks effective genetic resources for fruit tree dwarfing breeding, resulting in high orchard management costs and difficulty in achieving high-density cultivation to increase yield.

Method used

The Pybbx24 gene, which is associated with dwarfing in pear trees and belongs to the B-Box family, was cloned and identified. By constructing a recombinant expression vector and overexpressing the gene in Arabidopsis thaliana and tobacco, its function of promoting plant dwarfing was verified.

Benefits of technology

It provides new genetic resources for dwarfing fruit tree breeding, reduces agricultural costs, achieves the effect of dwarfing plants, and provides the possibility of gene editing for high-density cultivation, thereby reducing orchard management costs.

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Abstract

本发明公开了梨Pybbx24基因及其矮化功能的应用。一种分离自‘红早酥’及其F1分离后代的矮化系具有促进梨树植株矮化生长的转录因子Pybbx24,属于B‑Box家族成员,其核苷酸序列为SEQ ID No.1所示,其编码的氨基酸序列为序列表SEQ ID No.2所示。通过农杆菌介导遗传转化方法将Pybbx24基因稳定转化拟南芥和大叶烟草。经分子生物学功能验证,表明本发明克隆的Pybbx24基因具有调控植株矮化生长的功能。该基因的发现,为分子育种提供更高效地途径,为矮化树型的分子育种提供新的基因资源,为实施绿色农业提供新的遗传资源,该遗传资源的开发利用有利于降低农业成本和实现环境友好。
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