Biopesticide containing amino acid liquid and method for preparing the same

Abstract

The application relates to the technical field of pesticides, and particularly discloses a biological pesticide containing amino acid liquid agent and a preparation method thereof. The biological pesticide is formed by mixing an A agent and a B agent, the A agent comprises the following components in parts by weight: 80-90 parts of boric acid, 30-34 parts of zinc sulfate, 485-505 parts of water, 50-60 parts of a polyhydric alcohol, 10-12 parts of an emulsifier, 145-157 parts of the amino acid liquid agent, 200-208 parts of a polyglutamic acid liquid fertilizer, and 0.6-1.4 parts of magnesium sodium ethylenediaminetetraacetate; the B agent is a chitosan sustained-release agent, the chitosan sustained-release agent contains 30-50% of chitosan based on the weight of the A agent, and the chitosan sustained-release agent is a montmorillonite powder loaded with chitosan. The chitosan released by the chitosan sustained-release agent can kill the rice seedling disease pathogenic bacteria in a rice field to a certain extent, so that the rice seedling disease can be inhibited to a certain extent, and the damage of the rice seedling disease to the rice field can be reduced.

Description

Technical Field

[0001] This application relates to the field of pesticide technology, and more specifically, to a biological pesticide containing an amino acid liquid and a method for its preparation. Background Technology

[0002] Biological pesticides are pesticide formulations synthesized from organisms themselves or their secretions, which can protect target crops from harmful organisms. Compared with chemical pesticides, biological pesticides have advantages such as less pollution, wider availability, and less likelihood of developing resistance, thus having broad application prospects.

[0003] Amino acid liquid is a biological agent with amino acids as its main component, produced by bio-fermentation. It can be compounded with various fertilizers to form water-soluble fertilizer solutions. One related technology provides a water-soluble fertilizer containing amino acid liquid, comprising the following components by weight: 80 parts boric acid, 30 parts zinc sulfate, 485 parts water, 50 parts polyol, 10 parts emulsifier, 145 parts amino acid liquid, 200 parts 3% polyglutamic acid liquid fertilizer, and 0.6 parts sodium magnesium ethylenediaminetetraacetate.

[0004] Regarding the aforementioned technologies, the inventors believe that although the water-soluble fertilizers in these technologies contain added amino acid liquids, amino acids are also a good source of organic nitrogen for rice fields infected with rice bakanae disease. Applying the water-soluble fertilizers in these technologies would aggravate the condition of rice bakanae disease, thus limiting the application of these water-soluble fertilizers in rice fields. Summary of the Invention

[0005] For rice paddies infected with rice bakanae disease, amino acids are also a good source of organic nitrogen. Applying water-soluble fertilizers using related technologies to such paddies can exacerbate the rice bakanae disease, limiting the application of these water-soluble fertilizers in rice paddies. To overcome this deficiency, this application provides a biological pesticide containing amino acid liquid and its preparation method.

[0006] In a first aspect, this application provides a biological pesticide containing an amino acid liquid, employing the following technical solution: A biological pesticide containing an amino acid liquid, wherein the biological pesticide is composed of a mixture of agent A and agent B, wherein agent A comprises the following components in parts by weight: 80-90 parts boric acid, 30-34 parts zinc sulfate, 485-505 parts water, 50-60 parts polyol, 10-12 parts emulsifier, 145-157 parts amino acid liquid, 200-208 parts polyglutamic acid liquid fertilizer, and 0.6-1.4 parts ethylenediaminetetraacetic acid magnesium sodium; agent B is a chitosan slow-release agent, wherein the chitosan contained in the chitosan slow-release agent is 30-50% of the weight of agent A, and the chitosan slow-release agent is montmorillonite powder loaded with chitosan.

[0007] By adopting the above technical solution, this application adds a chitosan slow-release agent to the water-soluble fertilizer in related technologies, thus obtaining a biological pesticide that combines the effects of water-soluble fertilizer. The chitosan slow-release agent of this application uses montmorillonite as the carrier of chitosan, and montmorillonite has slow-release properties. After mixing agent A and agent B to form a biological pesticide and diluting it, montmorillonite can achieve continuous release of chitosan. Chitosan can kill the pathogens of rice bakanae disease, and the slow-release property of montmorillonite helps reduce the possibility of chitosan loss after irrigation and water exchange in paddy fields. The addition of the chitosan slow-release agent can play a certain inhibitory role in rice bakanae disease, thereby helping to reduce the damage caused by rice bakanae disease to paddy fields.

[0008] Preferably, the chitosan sustained-release agent is prepared according to the following method: (1) Sodium montmorillonite is mixed with water to prepare a dispersion with a mass fraction of 2% montmorillonite. Then the pH of the dispersion is adjusted to 5 and set aside. Chitosan is mixed with acetic acid solution, and after stirring and standing, a chitosan solution with a mass fraction of 2% is obtained and set aside. (2) Add the chitosan solution to the dispersion and heat it in a water bath at 60°C for 10 hours. After washing with the washing liquid, the product is centrifuged, dehydrated and freeze-dried to obtain the chitosan sustained-release agent.

[0009] By adopting the above technical solution, this application first adjusts the pH of sodium montmorillonite in the dispersion, dissolves chitosan in acetic acid, and then mixes the two under constant temperature water bath conditions to prepare montmorillonite powder loaded with chitosan, namely chitosan slow-release agent.

[0010] Preferably, when preparing the dispersion, zinc sulfate and sodium montmorillonite are added together to the water.

[0011] When using the above-mentioned technical solution, montmorillonite, as a carrier for chitosan, adsorbs zinc sulfate from biopesticides, leading to a decrease in the zinc ion concentration in the biopesticides. However, by adding zinc sulfate during the preparation of the dispersion, montmorillonite can pre-adsorb zinc sulfate, helping to reduce the magnitude of the decrease in zinc sulfate concentration in the biopesticides.

[0012] Preferably, the amount of zinc sulfate used is 3-6% of the weight of sodium montmorillonite.

[0013] By adopting the above technical solution, the amount of zinc sulfate was optimized, which helps montmorillonite to fully absorb zinc sulfate during the preparation of chitosan slow-release agent, thereby reducing the impact of the addition of chitosan slow-release agent on the concentration of zinc sulfate.

[0014] Preferably, the washing liquid comprises water and zinc sulfate.

[0015] By adopting the above technical solution, in the process of preparing chitosan sustained-release agent according to the above method, if pure water is used as the washing solution, some zinc sulfate on the surface of montmorillonite will be lost during the washing process. However, by using a washing solution prepared from zinc sulfate and water, the loss of zinc sulfate on the surface of montmorillonite during the washing process can be reduced, thereby helping to reduce the impact of the addition of chitosan sustained-release agent on the zinc sulfate concentration in biopesticides.

[0016] Preferably, the washing solution is prepared from a saturated zinc sulfate solution at 60°C and zinc sulfate crystals.

[0017] By adopting the above technical solution, in the washing solution made by mixing zinc sulfate saturated solution and zinc sulfate crystals at 60℃, zinc sulfate has reached saturation and is in an excess state, making it difficult to continue dissolving zinc sulfate during the washing process. This helps to reduce the loss of zinc sulfate adsorbed in montmorillonite during the washing process.

[0018] Preferably, the polyol is mannitol.

[0019] By adopting the above technical solution, mannitol can chelate with boric acid. After mannitol chelates with boric acid, the pK value of boric acid decreases from 9.25 to 5.15, which is beneficial to improving the solubility of boric acid in biological pesticides.

[0020] Secondly, this application provides a method for preparing a biological pesticide containing amino acid liquid, using the following technical solution.

[0021] A method for preparing a biological pesticide containing amino acid liquid includes the following steps: (1) Mix water, polyol, emulsifier and amino acid liquid, then add boric acid and zinc sulfate to the mixture in sequence, and stir in a constant temperature water bath at 60℃ for 12h to obtain the base liquid; weigh out the chitosan sustained-release agent as agent B for later use; (2) Mix the polyglutamic acid liquid fertilizer with the base solution and stir. After adjusting the pH, agent A is obtained. (3) Mix agent A and agent B to obtain a biological pesticide containing amino acid liquid.

[0022] By adopting the above technical solution, this application first mixes the components with relatively small impact on viscosity with water to prepare a base liquid, then adds polyglutamic acid liquid fertilizer to the base liquid to obtain Agent A, and then mixes Agent A with chitosan slow-release agent as Agent B and stirs to obtain a biological pesticide containing amino acid liquid.

[0023] Preferably, in step (2) of preparing the biopesticide, the pH is adjusted so that the pH of agent A after being diluted 20 times is 5.8-6.2.

[0024] By adopting the above technical solution, the pH value of agent A after being diluted 20 times was optimized, which is conducive to chitosan fully exerting its killing effect on the pathogens causing rice seedling blight.

[0025] Preferably, the components of Agent A also include a thickener. In step (2) of preparing the biopesticide, the thickener, polyglutamic acid liquid fertilizer and base liquid are mixed together. The thickener is hydroxyethyl cellulose, and the amount of hydroxyethyl cellulose is 10-15 times the weight of polyglutamic acid contained in the polyglutamic acid liquid fertilizer.

[0026] By employing the above technical solution, both hydroxyethyl cellulose and polyglutamic acid are long-chain polymers rich in polar groups. Their main chains can entangle with each other in aqueous solution through intermolecular forces, and the polar groups also exhibit hydrogen bonding. The synergistic effect between polyglutamic acid and hydroxyethyl cellulose can more effectively increase the viscosity of the biopesticide dilution, and this increased viscosity is beneficial for improving the fixation effect of the biopesticide dilution on soil particles and crop plant surfaces.

[0027] In summary, this application has the following beneficial effects: 1. The biological pesticide of this application contains a chitosan slow-release agent. The chitosan released by the chitosan slow-release agent can kill the pathogens of rice bakanae disease in paddy fields to a certain extent, thereby inhibiting rice bakanae disease and helping to reduce the damage caused by rice bakanae disease to paddy fields.

[0028] 2. In this application, the preferred washing solution is prepared from a saturated zinc sulfate solution at 60°C and zinc sulfate crystals. In this washing solution, the zinc sulfate is already in excess, making it difficult to dissolve further during the washing process. Therefore, the washing agent prepared in this way is less likely to dissolve the zinc sulfate on the surface of montmorillonite, thus helping to reduce the loss of zinc sulfate from the surface of montmorillonite during the washing process. Detailed Implementation

[0029] The present application will be further described in detail below with reference to the embodiments, preparation examples and comparative examples. The raw materials involved in the present application can all be obtained commercially.

[0030] Preparation example of chitosan sustained-release agent The following explanation uses Preparation Example 1 as an example.

[0031] Preparation Example 1 In this preparation example, the chitosan sustained-release agent was prepared according to the following method: (1) Sodium-based montmorillonite is mixed with water to prepare a dispersion with a mass fraction of 2% montmorillonite. The pH of the dispersion is then adjusted to 5 and set aside. Chitosan with an average molecular weight of 1400 is mixed with a 2% acetic acid solution. After stirring and standing, a chitosan solution with a mass fraction of 2% is obtained and set aside. In this step, the weight ratio of chitosan to montmorillonite is 2:1. (2) Add the chitosan solution to the dispersion and heat it in a water bath at 60°C for 10 hours. After washing with the washing liquid, the product is centrifuged, dehydrated and freeze-dried to obtain the chitosan sustained-release agent. In this step, the washing liquid used is pure water.

[0032] Preparation Example 2 The difference between this preparation example and Preparation Example 1 is that, when preparing the dispersion, zinc sulfate was added together with sodium montmorillonite to the water, and the amount of zinc sulfate used was 2% of the weight of sodium montmorillonite.

[0033] As shown in Table 2, the difference between Preparation Examples 2-6 lies in the percentage of zinc sulfate used relative to the weight of sodium montmorillonite (hereinafter referred to as zinc sulfate percentage).

[0034] Table 1. Proportion of Zinc Sulfate Preparation Example 7 The difference between this preparation example and preparation example 6 is that the washing solution is a zinc sulfate solution prepared at 60°C with a saturation of 20%.

[0035] Preparation Example 8 The difference between this preparation example and preparation example 7 is that the washing solution is made by mixing a saturated zinc sulfate solution prepared at 60°C and zinc sulfate heptahydrate at a weight ratio of 20:1. Example

[0036] Examples 1-5 The following description uses Example 1 as an example.

[0037] Example 1 This embodiment provides a biological pesticide composed of a mixture of agent A and agent B. Agent A comprises the following components: 80 kg boric acid, 30 kg zinc sulfate, 485 kg water, 50 kg polyol, 10 kg emulsifier, 145 kg amino acid liquid, 200 kg polyglutamic acid liquid fertilizer, and 0.6 kg ethylenediaminetetraacetic acid magnesium sodium. Agent B is the chitosan slow-release agent from Preparation Example 1. The amount of chitosan slow-release agent added is determined according to the weight percentage of chitosan in agent A (hereinafter referred to as chitosan percentage). In this embodiment of the biological pesticide, the chitosan percentage is 30%.

[0038] In this embodiment, mannitol is selected as the polyol, AEO-9 is used as the emulsifier, the amino acid liquid has an amino acid mass fraction of 70%, and the polyglutamic acid liquid fertilizer is an aqueous solution with a polyglutamic acid mass concentration of 3%.

[0039] In this embodiment, the amino acid-containing liquid biopesticide is prepared according to the following steps: (1) Mix water, polyol, emulsifier and amino acid liquid, then add boric acid and zinc sulfate to the mixture in sequence, and stir in a constant temperature water bath at 60℃ for 12h to obtain the base liquid; weigh out the chitosan sustained-release agent as agent B for later use; (2) Mix the polyglutamic acid liquid fertilizer with the base solution and stir to obtain Agent A. Adjust the pH so that the pH of Agent A after being diluted 20 times is 5.5. (3) Mix agent A and agent B to obtain a biological pesticide containing amino acid liquid.

[0040] As shown in Table 2, the main difference between Examples 1-5 lies in the different raw material ratios of the biological pesticides.

[0041] Table 2 Raw material ratios for biological pesticides Examples 6-11 As shown in Table 3, the difference between Examples 6-11 and Example 5 is that the preparation methods of the chitosan sustained-release agent are different.

[0042] Table 3 Examples of chitosan sustained-release formulations Example 5 Preparation Example 1 Example 6 Preparation Example 2 Example 7 Preparation Example 3 Example 8 Preparation Example 4 Example 9 Preparation Example 5 Example 10 Preparation Example 6 Example 11 Preparation Example 7 Example 12 Preparation Example 8 Example 13 The difference between this embodiment and embodiment 12 is that in step (2) of preparing the biopesticide, the thickener is mixed together with the polyglutamic acid liquid fertilizer and the base liquid. The thickener is hydroxyethyl cellulose, and the amount of thickener used is 10 times the weight of polyglutamic acid contained in the polyglutamic acid liquid fertilizer.

[0043] Example 14 The difference between this comparative example and Example 13 is that in step (2) of preparing the biopesticide, the pH was adjusted so that the pH of the biopesticide after being diluted 20 times was 5.8.

[0044] Example 15 The difference between this comparative example and Example 13 is that in step (2) of preparing the biopesticide, the pH was adjusted so that the pH of the biopesticide after being diluted 20 times was 6.0.

[0045] Example 16 The difference between this comparative example and Example 13 is that in step (2) of preparing the biopesticide, the pH was adjusted so that the pH of the biopesticide after being diluted 20 times was 6.2.

[0046] Example 17 The difference between this comparative example and Example 13 is that in step (2) of preparing the biopesticide, the pH is adjusted so that the pH of the biopesticide after being diluted 20 times is 6.5.

[0047] Comparative Example Comparative Example 1 The difference between this comparative example and Example 1 is that chitosan slow-release agent is not added when formulating the biopesticide.

[0048] Comparative Example 2 The difference between this comparative example and Example 12 is that the mass of polyglutamic acid liquid fertilizer in Agent A is replaced with pure water.

[0049] Performance testing methods I. Detection of pathogen-killing effect Tested pesticides: biological pesticides from Examples 1-17 and Comparative Examples 1-2, totaling 19 groups.

[0050] Test method: (1) Select one group from the 19 groups of tested reagents, dilute it 20 times with pure water to obtain a diluted solution for later use; (2) Add 12 mL of potato sucrose medium to a petri dish with a diameter of 9 cm to make a plate. Inoculate a 5-day-old rice bakanae disease PDA spore strip with a diameter of 0.5 cm in the center of the plate. Place a set of four steel tubes with a capacity of 0.3 mL and a diameter of 0.4 cm on the plate, symmetrically placed with the center of the petri dish. Add the diluent obtained in step (1) to the steel tubes. Use distilled water as the diluent for the control group and incubate at 28°C. (3) When the hyphae grow to the same length as the control group steel pipe, the diameter of the inhibition zone is measured. The diameter of the inhibition zone is twice the distance from the center of the treated steel pipe to the nearest hyphae. The average diameter of the inhibition zone measured in four steel pipes in a group is taken as the diameter of the inhibition zone of that group.

[0051] After obtaining the measurement results of the inhibition zone diameter of 19 groups of tested agents, the ratio of the inhibition zone diameter of Examples 1-17 and Comparative Examples 1-2 to the inhibition zone diameter of Comparative Example 1 was calculated. This ratio was recorded as the relative inhibition rate. The results are shown in Table 4.

[0052] Table 4 Relative Antibacterial Rate Example 1 142.7 Example 11 167.1 Example 2 147.5 Example 12 167.3 Example 3 154.8 Example 13 168.4 Example 4 160.2 Example 14 172.4 Example 5 166.9 Example 15 175.7 Example 6 167.2 Example 16 173.5 Example 7 167.0 Example 17 170.2 Example 8 167.1 Comparative Example 1 100.0 Example 9 167.3 Comparative Example 2 100.4 Example 10 167.2 / / II. Viscosity Evaluation Tested pesticides: Biological pesticides of Example 13 and Comparative Example 2.

[0053] Experimental method: The biopesticides of Example 13 and Comparative Example 2 were each diluted 20 times to obtain two diluents. The viscosity difference between the diluent of Example 13 and the diluent of Comparative Example 2 was then measured and calculated. The ratio of this viscosity difference to the viscosity measured in Comparative Example 2 was then calculated. The result was expressed as a percentage as 16.8%.

[0054] III. Evaluation of sustained-release effect: Biological pesticides of Examples 5-12 and chitosan sustained-release agents of Preparation Examples 1-8 (Zinc ions were not added during the preparation of chitosan sustained-release agent of Preparation Example 1, so zinc ion content was not tested in Example 5).

[0055] Methods for detecting chitosan content: Weigh the dried sample and record the initial weight. Then, ignite it at 800℃ to constant weight and record the residual weight. Calculate the difference between the initial weight and the residual weight. Based on this difference, subtract the weight of the water of crystallization removed by montmorillonite (the weight of the water of crystallization can be measured by calcining montmorillonite under the same conditions) to obtain the chitosan loss on ignition. Calculate the percentage of chitosan loss on ignition to the initial weight and record this percentage as the chitosan content.

[0056] Methods for detecting zinc ion content: Weigh the dried sample and record the initial weight. Then digest the dried sample with aqua regia and measure the zinc ion content with an ICP analyzer. Calculate the percentage of zinc ion weight to the initial weight and record this percentage as the zinc ion content.

[0057] Test method: (1) The chitosan sustained-release agents prepared in Examples 1-8 were dried at 105°C to constant weight and used as dried samples. The zinc ion content and chitosan content were determined in the manner described above and recorded as the initial chitosan content and the initial zinc ion content. (2) Using the same batch of chitosan sustained-release agent as in each preparation example in step (1), prepare the tested biological pesticide according to the formulation and steps described in Examples 5-12, and let it stand for later use. (3) After the tested biological pesticide was left to stand for 7 days, it was kept at 60℃ for 30 minutes and then filtered while hot. The filter residue was washed with pure water at 60℃ and dried at 105℃ to constant weight. The weight of the filter residue was then weighed and used as the initial weight of the dried sample. The chitosan content and zinc ion content were tested in the manner described above and recorded as the chitosan residual content and zinc ion residual content.

[0058] (4) Calculate the ratio of the residual chitosan content to the initial chitosan content, and record it as the chitosan residual rate; calculate the ratio of the residual zinc ion content to the initial zinc ion content, and record it as the zinc ion residual rate. The calculation results of the chitosan residual rate and the zinc ion residual rate are shown in Table 5.

[0059] Table 5 Chitosan Residual Rate and Zinc Ion Residual Rate As can be seen from Examples 1-17 and Comparative Example 1, and Table 4, the relative antibacterial rates of Examples 1-17 are all greater than those of Comparative Example 1. This indicates that the present application achieves the killing of rice bakanae disease pathogens by adding chitosan slow-release agent, which helps to overcome the defect that the application of water-soluble fertilizer containing amino acid liquid causes the rice bakanae disease to worsen.

[0060] As can be seen from Examples 13-17 and Table 4, when the biological pesticide of this application is diluted 20 times, the diluted solution with a pH of 5.8-6.2 has a good killing effect on rice seedling blight.

[0061] Combining Example 13 and Comparative Example 2 with the viscosity evaluation results, it can be seen that the viscosity of the diluted solution in Example 13 is significantly higher than that in Comparative Example 2. This indicates that the synergistic effect between polyglutamic acid and hydroxyethyl cellulose can more fully improve the viscosity of the diluted biopesticide, thus improving the fixation effect of the diluted biopesticide on soil particles and crop plant surfaces.

[0062] As can be seen from Examples 5-12 and Table 5, after the chitosan sustained-release agents of Preparation Examples 1-8 were formulated into the biopesticides of Examples 5-12, the chitosan content decreased after 7 days of standing, indicating that the chitosan sustained-release agent of this application can achieve sustained release of chitosan in biopesticides.

[0063] The zinc ion residual rate measured in Example 6 exceeded 100%, indicating that the chitosan slow-release agent prepared in Example 2 absorbed zinc ions in the biopesticide, which in turn led to a decrease in the zinc ion concentration in the biopesticide. Based on the zinc ion residual rates measured in Examples 6-10, it can be seen that when the zinc sulfate content added during the preparation of the chitosan slow-release agent is controlled at 3-6% of the weight of sodium montmorillonite, the amount of zinc ions absorbed by the chitosan slow-release agent in the biopesticide is relatively small. Even in undiluted biopesticides, zinc ions can be released, thus preventing a decrease in the zinc ion concentration in the biopesticide. Furthermore, considering Examples 10-12, it can be seen that as the zinc sulfate concentration in the washing solution increases, the measured zinc ion residual rate further decreases, indicating that using a zinc sulfate solution as the washing solution helps to significantly reduce the amount of zinc ions absorbed by the chitosan slow-release agent in the biopesticide.

[0064] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A biological pesticide containing an amino acid liquid, characterized in that, The biological pesticide is a mixture of agent A and agent B. Agent A comprises the following components by weight: 80-90 parts boric acid, 30-34 parts zinc sulfate, 485-505 parts water, 50-60 parts polyol, 10-12 parts emulsifier, 145-157 parts amino acid liquid, 200-208 parts polyglutamic acid liquid fertilizer, and 0.6-1.4 parts sodium magnesium ethylenediaminetetraacetate. Agent B is a chitosan slow-release agent, containing 30-50% chitosan by weight of agent A, and is montmorillonite powder loaded with chitosan. The polyol is mannitol. The chitosan slow-release agent is prepared according to the following method: (1) Sodium montmorillonite is mixed with water to prepare a dispersion with a mass fraction of 2% montmorillonite. Then the pH of the dispersion is adjusted to 5 and set aside. Chitosan is mixed with acetic acid solution, and after stirring and standing, a chitosan solution with a mass fraction of 2% is obtained and set aside. (2) Add the chitosan solution to the dispersion and heat it in a water bath at 60°C for 10 hours. After washing with the washing liquid, the product is centrifuged, dehydrated and freeze-dried to obtain the chitosan sustained-release agent.

2. The amino acid-containing liquid biopesticide according to claim 1, characterized in that, When preparing the dispersion, zinc sulfate and sodium montmorillonite are added together to the water.

3. The amino acid-containing liquid biopesticide according to claim 2, characterized in that, The amount of zinc sulfate used is 3-6% of the weight of sodium montmorillonite.

4. The amino acid-containing liquid biopesticide according to claim 2, characterized in that, The washing solution comprises water and zinc sulfate.

5. The amino acid-containing liquid biopesticide according to claim 4, characterized in that, The washing solution is prepared from a saturated zinc sulfate solution at 60°C and zinc sulfate crystals.

6. The method for preparing the amino acid-containing liquid biopesticide according to any one of claims 1-5, characterized in that, Includes the following steps: (1) Mix water, polyol, emulsifier and amino acid liquid, then add boric acid and zinc sulfate to the mixture in sequence, and stir in a constant temperature water bath at 60°C for 12 hours to obtain the base liquid; weigh out the chitosan sustained-release agent as agent B for later use; (2) Mix the polyglutamic acid liquid fertilizer with the base solution and stir. After adjusting the pH, agent A is obtained. (3) Mix agent A and agent B to obtain a biological pesticide containing amino acid liquid.

7. The method for preparing the amino acid-containing liquid biopesticide according to claim 6, characterized in that, In step (2) of preparing the biopesticide, the pH is adjusted so that the pH of agent A after being diluted 20 times is 5.8-6.

2.

8. The method for preparing the amino acid-containing liquid biopesticide according to claim 6, characterized in that, The components of Agent A also include a thickener. In step (2) of preparing the biopesticide, the thickener, polyglutamic acid liquid fertilizer and base liquid are mixed together. The thickener is hydroxyethyl cellulose, and the amount of hydroxyethyl cellulose is 10-15 times the weight of polyglutamic acid contained in the polyglutamic acid liquid fertilizer.

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