一种抗交叉污染的总胆红素测定试剂盒
By improving the solubility of indirect bilirubin in acidic solutions and preparing reagents R1 and R2, the problem of cross-contamination and interference in total bilirubin assay reagents was solved, achieving reagent stability and accuracy of assay results, especially in the testing of total bilirubin and high-density lipoprotein cholesterol in fully automated biochemical analyzers.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- QINGDAO HIGHTOP BIOTECH
- Filing Date
- 2023-10-25
- Publication Date
- 2026-07-17
AI Technical Summary
Existing reagents for total bilirubin assay are susceptible to cross-contamination and interference, leading to inaccurate results. This is especially true when testing total bilirubin and high-density lipoprotein cholesterol in fully automated biochemical analyzers, where contamination carried by the reagent needle and residues from cleaning the reaction cup result in inaccurate absorbance. Furthermore, samples containing cetyltrimethylammonium bromide exhibit abnormal reaction curves.
The solubility of indirect bilirubin was improved by using an acidic solution under surfactant conditions. Total bilirubin was oxidized by vanadate to generate biliverdin. Reagents R1 and R2 were prepared. Reagent R1 contained buffer, ionic raw materials, surfactant and preservative, and reagent R2 contained buffer and EDTA. The volume ratio of R1:R2 was 4:1. The specific components and concentrations were optimized to eliminate cross-contamination and interference.
It effectively eliminates cross-contamination of high-density lipoprotein cholesterol, avoids cross-contamination between reagents and turbidity interference, ensures the accuracy of the measurement results and the stability of the reagents, shortens the reaction time, reduces the influence of serum interfering substances, and does not affect the normal operation of the analytical instrument.
Smart Images
Figure CN117420088B_ABST