A plant-based composition with soothing properties for use in cosmetics and its application thereto.

Abstract

This invention discloses a plant composition with soothing effects for cosmetic use and its application. The active ingredients of the composition are: tuberose, scutellaria baicalensis, centella asiatica, chamomile, and sage. The plant composition of this invention achieves multi-target skin soothing and barrier repair functions through the synergistic effect of the compounding of its components. In soothing efficacy tests, this composition effectively inhibits the release of allergic mediators and inflammatory factors, exhibiting significant antipruritic, redness-reducing, and swelling-reducing effects. Simultaneously, this composition is mild and safe, effectively improving the anti-allergic and soothing properties of cosmetics, making cosmetics safer and more effective.

Description

Technical Field

[0001] This invention relates to a plant composition, and more specifically to a plant composition for cosmetic use with soothing effects and its application. Background Technology

[0002] In the manufacturing process of cosmetics, in addition to thickening systems and active ingredients in the formula, preservatives, fragrances, pigments, and surfactants are added. These substances can easily become allergens for skin allergies. Furthermore, some people have damaged skin barrier function, leaving their skin in a highly sensitive, sub-healthy state. These individuals are more prone to cosmetic allergies, experiencing symptoms such as stinging, burning, tightness, itching, peeling, and redness after using cosmetics. Cosmetic allergies not only affect normal work and study but can also lead to complications in severe cases.

[0003] To reduce the skin irritation caused by allergens in cosmetics, anti-allergens are often added to cosmetic formulations. Commonly used anti-allergens in cosmetics include bisabolol, allantoin, paeonol, purslane extract, and comfrey extract. These anti-allergens all have certain soothing and anti-allergic effects, but they also have certain drawbacks. They often target only one specific area, resulting in weak anti-allergic efficacy or recurring allergic symptoms. Some anti-allergens have poor stability or low solubility, and excessive addition can even cause irritation. For example, allantoin has a certain astringent effect on the skin, and its addition is limited to 0.1-0.2%; adding too much can cause irritation. Bisabolol relieves skin inflammation by inhibiting the release of leukotrienes and interleukin-1 allergy mediators, but it does not have the effect of repairing and reconstructing the skin barrier. Paeonol has poor water solubility and an unpleasant odor, limiting its application in cosmetics. Purslane extract carries the risk of photosensitive substance residue. Comfrey extract is suitable for fat-soluble systems, but it has the disadvantage of easily fading and decomposing under light.

[0004] Therefore, providing a cosmetic additive with multi-target anti-allergic effects while ensuring its good stability, safety and compatibility is an urgent problem to be solved in the cosmetics industry. Summary of the Invention

[0005] The purpose of this invention is to overcome at least one deficiency of the prior art and to provide a plant composition with soothing effects for use in cosmetics and its application.

[0006] The technical solution adopted in this invention is: In a first aspect, the present invention provides a plant composition for cosmetic use with a soothing effect, wherein the active ingredients are: tuberose, scutellaria baicalensis, centella asiatica, chamomile, and sage.

[0007] In some instances, the active ingredient is an aqueous extract of the raw material.

[0008] In some examples, the mass ratio of the active ingredient raw materials is as follows: 12-18 parts tuberose, 1-7 parts scutellaria baicalensis, 1-7 parts centella asiatica, 1-7 parts chamomile, and 1-7 parts sage. A preferred mass ratio is 12-18 parts tuberose, 2-6 parts scutellaria baicalensis, 2-6 parts centella asiatica, 3-5 parts chamomile, and 3-5 parts sage.

[0009] In some examples, the mass ratio of the raw materials of its active ingredients is: 15 parts tuberose, 4 parts scutellaria baicalensis, 4 parts centella asiatica, 4 parts chamomile, and 4 parts sage.

[0010] In some instances, the method for preparing the plant composition includes the following steps: 1) After pulverizing the tuberose herb, place it in an extraction tank and add 10 to 30 times the weight of the herb in deionized water for extraction. Separate the solid and liquid components of the extract. Decolorize the extract with a macroporous weak base anion exchange resin. Separate and concentrate the decolorized solution with an ultrafiltration membrane with a pore size of 20 kD. Dry the concentrate to obtain tuberose polysaccharide. 2) Grind Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly, and obtain compound herbal powder. 3) After pulverizing the compound medicinal materials obtained in step 2, place them in an extraction tank, add 10-30% of deionized water by weight of the compound medicinal materials for extraction, perform solid-liquid separation on the extracted material, filter the obtained extract with a ceramic membrane, purify the obtained filtrate with an ultrafiltration membrane, concentrate the obtained purified liquid with a nanofiltration membrane, and the concentrated liquid obtained is the compound extract. 4) Place the tuberose polysaccharide from step 1 and the compound extract from step 3 into an extraction vessel, stir to dissolve, add the extractant to the solution, stir to mix evenly, let stand for 12-24 hours, and take the supernatant to obtain the plant composition.

[0011] In some instances, the parameters for the water extraction steps 1) and 3) are: extraction speed of 60-90 rpm, extraction temperature of 25-60℃, and extraction time of 60-150 min.

[0012] In some instances, the mass of macroporous weak base anion exchange resin added in step 1) is 10-20% of the mass of the extract.

[0013] In some instances, the ceramic membrane pore size in step 3) is 200–800 nm, the ultrafiltration membrane pore size is 3 kD–10 kD, the nanofiltration membrane pore size is 80–200 Dal, and the specific gravity of the resulting concentrate is 1.080–1.160.

[0014] In some instances, the extractant in step 4) is selected from at least one of ethanol, propylene glycol, butanediol, pentanediol, and hexanediol, and the mass of the extractant added is 15-55% of the mass of the solution.

[0015] Secondly, a skin care product with soothing effects includes excipients and active ingredients, said active ingredients including the plant composition described in the first aspect.

[0016] In some instances, the amount of the plant composition added is 0.1% to 10% by mass.

[0017] In some instances, the skincare products are ointments, creams, lotions, aerosols, lotions, gels, shampoos, shower gels, and facial cleansers.

[0018] The beneficial effects of this invention are: 1) The plant composition of the present invention achieves synergistic effects through the combination of its components, thereby achieving multi-target skin soothing and barrier repair functions. It can effectively inhibit the activity of hyaluronidase allergy mediators and the release of IL-6 inflammatory factors, and has significant antipruritic, redness-reducing and swelling-reducing effects.

[0019] 2) The plant composition of the present invention is mild and safe, and does not contain hormones, antibiotics or other compounds. It can effectively improve the anti-allergy and soothing properties of cosmetics, making cosmetics safer and more effective. Attached Figure Description

[0020] Figure 1 These are photos of skin symptoms before and 60 minutes after applying the test substance.

[0021] Figure 2 This is the result of the chorioallantoic membrane test. Detailed Implementation

[0022] The following disclosure provides many different implementations or examples for different ways of implementing the present invention.

[0023] Tuberose, also known as moonflower, night-blooming jasmine, and jade hairpin flower, is a plant belonging to the genus *Tuberose* in the family Amaryllidaceae. Polianthes tuberosa L. The root of [the plant / organism] is cool in nature, slightly sweet and bland in taste, and enters the lung, heart, and liver meridians. It has the effects of detoxification and blood stasis removal, and is often used to treat mosquito bites and various skin swellings and pains.

[0024] Scutellaria baicalensis, also known as Scutellaria baicalensis root or Scutellaria baicalensis root, is a plant belonging to the Lamiaceae family. Scutellaria baicalensis GeorgiThe dried root of [the plant]. It is cold in nature and bitter in taste. It enters the lung, gallbladder, spleen, large intestine, and small intestine meridians. It has the effects of clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding, and calming the fetus. It is often used for damp-heat syndrome, summer-heat syndrome, chest tightness and nausea, damp-heat fullness, diarrhea, jaundice, lung-heat cough, high fever and thirst, blood-heat vomiting and nosebleeds, carbuncles and boils, and threatened abortion.

[0025] Centella asiatica, also known as snowball grass, is a plant belonging to the Apiaceae family. Centella asiatica L.Urb. The dried whole herb of [plant name missing]. It is cold in nature, bitter and pungent in taste, and enters the liver, spleen, and kidney meridians. It has the effects of clearing heat and dampness, detoxifying and reducing swelling, and can be used for damp-heat jaundice, heatstroke diarrhea, urinary stones and hematuria, carbuncles and boils, and injuries from falls.

[0026] Chamomile, also known as baby chamomile or marigold, is an annual herbaceous plant belonging to the genus Chamomile in the family Asteraceae. Matricaria chamomilla L. The flower of the plant. It is cool in nature, pungent and slightly bitter in taste, and enters the lung meridian. It has the effects of clearing heat and detoxifying, relieving cough and asthma, and dispelling wind and dampness. It is mainly used to treat colds with fever, sore throat, cough and asthma due to lung heat, swelling and pain due to heat arthralgia, and sores.

[0027] Medicinal sage is a perennial herbaceous plant belonging to the genus *Salvia* of the Lamiaceae family. Salvia officinalisLinn. The whole herb of [plant name missing]. It is neutral in nature, pungent and bitter in taste, and enters the heart, lung, liver, large intestine, and bladder meridians. It has the effects of clearing heat and dampness, promoting blood circulation and regulating menstruation, and detoxifying and reducing swelling. It can be used to treat jaundice, dysentery, damp-heat leukorrhea, irregular menstruation, dysmenorrhea, sores and boils, and traumatic injuries.

[0028] The mass fractions of the plant compositions in the various embodiments and comparative examples of this invention are shown in Table 1 below. Specifically, in Comparative Example 9, Scutellaria baicalensis was replaced with Phellodendron chinense, which has similar effects; both have similar effects in clearing heat and drying dampness, purging fire and detoxifying, cooling blood and stopping bleeding, clearing heat and reducing swelling, and purging fire and preserving yin. In Comparative Example 10, Rat tail was replaced with Rosemary, which has similar effects; both have various biological activities such as antibacterial, anti-inflammatory, and antioxidant properties.

[0029] Table 1 Example 1 15 4 4 4 4 Example 2 18 7 7 1 1 Example 3 12 1 1 7 7 Example 4 13 2 6 5 3 Example 5 16 6 2 3 5 Example 6 15 4 4 4 4 Example 7 15 4 4 4 4 Comparative Example 1 15 0 0 0 0 Comparative Example 2 0 4 0 0 0 Comparative Example 3 0 4 4 0 0 Comparative Example 4 0 0 4 4 0 Comparative Example 5 15 4 4 0 0 Comparative Example 6 0 4 4 4 0 Comparative Example 7 15 4 4 4 0 Comparative Example 8 0 4 4 4 4 Comparative Example 9 15 4 4 4 4 Comparative Example 10 15 4 4 4 4 Example 1

[0030] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through an 80-mesh sieve, place it in an extraction tank, add 15 times the weight of the herb in deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 40℃, and the extraction time is 90 min. Separate the extract using a plate centrifuge, collect the extract, add 1 times the weight of the herb in D301 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, then perform solid-liquid separation. Separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide. Step 2: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 30% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Example 2

[0031] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through a 60-mesh sieve, place it in an extraction tank, add 30 times the weight of the herb in deionized water for extraction, the extraction speed is 90 rpm, the extraction temperature is 60℃, and the extraction time is 150 min. Separate the extract using a plate centrifuge, collect the extract, add 1.5 times the weight of the herb in D315 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, perform solid-liquid separation, separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide; Step 2: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 10 times the weight of deionized water for extraction, the extraction speed is 60 rpm, the extraction temperature is 25℃, and the extraction time is 60 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through an 800 nm ceramic membrane, the obtained filtrate is purified through an 10 kD ultrafiltration membrane, and the purified liquid is concentrated through a 200 Dal nanofiltration membrane. Concentration is stopped when the specific gravity of the concentrated liquid is 1.160, and the obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 15% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 12 hours, and take the supernatant to obtain the plant composition. Example 3

[0032] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through a 100-mesh sieve, place it in an extraction tank, add 10 times the weight of the herb in deionized water for extraction, the extraction speed is 60 rpm, the extraction temperature is 25℃, and the extraction time is 60 min. Separate the extract using a plate centrifuge, collect the extract, add 0.5 times the weight of the herb in D380 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, perform solid-liquid separation, separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide; Step 2: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 30g of deionized water (by weight of the compound medicinal materials) for extraction. The extraction speed is 90 rpm, the extraction temperature is 60℃, and the extraction time is 150 min. The extracted materials are separated using a plate centrifuge. The resulting extract is filtered through a ceramic membrane with a pore size of 200 nm. The resulting filtrate is purified using an ultrafiltration membrane with a pore size of 3 kD. The purified liquid is concentrated using a nanofiltration membrane with a pore size of 80 nm. Concentration is stopped when the specific gravity of the concentrated liquid reaches 1.080. The resulting concentrated liquid is the compound extract. Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 55% by weight of the extractant propylene glycol to the solution, stir and mix evenly, let stand for 24 hours, and take the supernatant to obtain the plant composition. Example 4

[0033] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through a 70-mesh sieve, place it in an extraction tank, add 20 times the weight of the herb in deionized water for extraction, the extraction speed is 75 rpm, the extraction temperature is 50℃, and the extraction time is 120 min. Separate the extract using a plate centrifuge, collect the extract, add 1.2 times the weight of the herb in D311 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, then perform solid-liquid separation. Separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide. Step 2: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 70 rpm, the extraction temperature is 40℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.120, and the obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 30% by weight of the extractant propylene glycol to the solution, stir and mix evenly, let stand for 20 hours, and take the supernatant to obtain the plant composition. Example 5

[0034] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through a 90-mesh sieve, place it in an extraction tank, add 25 times the weight of the herb in deionized water for extraction, the extraction speed is 75 rpm, the extraction temperature is 55℃, and the extraction time is 120 min. Separate the extract using a plate centrifuge, collect the extract, add 0.8 times the weight of the herb in D301 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 2.5 hours, perform solid-liquid separation, separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide; Step 2: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 26 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 100 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 600 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.090. The obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 40% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Example 6

[0035] A method for preparing a plant composition with soothing effects for cosmetic use, except that step 1 involves separating and concentrating the decolorizing solution using an ultrafiltration membrane with a pore size of 30 kD, and step 3 involves a specific gravity of 1.060 for the concentrated solution, the other process parameters are the same as in Example 1. Example 7

[0036] A method for preparing a plant composition with soothing effects for cosmetic use, except that in step 3 the filtrate is purified by ultrafiltration membrane with a pore size of 30kD, and in step 4 60% by mass of butylene glycol is added to the solution as an extractant, the other process parameters are the same as in Example 1. Comparative Example 1

[0037] A method for preparing a plant composition with soothing effects for cosmetic use includes the following steps: weighing tuberose medicinal material, pulverizing it through an 80-mesh sieve, placing it in an extraction tank, adding 15 times the weight of deionized water for extraction, extracting at 80 rpm, at 40℃, and for 90 min. The extracted material is separated using a plate centrifuge, the extract is collected, and 1 times the weight of D301 macroporous weakly basic anion exchange resin is added for decolorization. The mixture is stirred and adsorbed for 3 hours, followed by solid-liquid separation. The decolorized solution is separated and concentrated using a 20kD ultrafiltration membrane, and the concentrated solution is dried under reduced pressure to obtain tuberose polysaccharide. Comparative Example 2

[0038] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Crush Scutellaria baicalensis into powder, place the powdered herb in an extraction tank, add 20% of the herb's mass of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted material is separated using a plate centrifuge, the obtained extract is filtered through a 500 nm ceramic membrane, the obtained filtrate is purified through a 5 kD ultrafiltration membrane, and the purified liquid is concentrated through a 100 Dal nanofiltration membrane. Concentration is stopped when the specific gravity of the concentrate is 1.010, and the obtained concentrate is Scutellaria baicalensis extract; Step 2: Place the extract from Step 1 into an extraction vessel, stir to dissolve, add 30% by weight of butylene glycol as an extractant to the solution, stir to mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 3

[0039] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Grind Scutellaria baicalensis and Centella asiatica into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 2: After pulverizing the compound medicinal materials obtained in Step 1, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 3: Place the compound extract from Step 2 into an extraction vessel, stir and dissolve it, then add 30% by weight of butylene glycol as an extractant to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 4

[0040] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Grind Centella asiatica and chamomile into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 2: After pulverizing the compound medicinal materials obtained in Step 1, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 3: Place the compound extract from Step 2 into an extraction vessel, stir and dissolve it, then add 30% by weight of butylene glycol as an extractant to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 5

[0041] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through an 80-mesh sieve, place it in an extraction tank, add 15 times the weight of the herb in deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 40℃, and the extraction time is 90 min. Separate the extract using a plate centrifuge, collect the extract, add 1 times the weight of the herb in D301 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, then perform solid-liquid separation. Separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide. Step 2: Crush Scutellaria baicalensis and Centella asiatica into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 30% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 6

[0042] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Grind Scutellaria baicalensis, Centella asiatica, and chamomile into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 2: After pulverizing the compound medicinal materials obtained in Step 1, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 3: Place the compound extract from Step 2 into an extraction vessel, stir and dissolve it, then add 30% by weight of butylene glycol as an extractant to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 7

[0043] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Weigh the tuberose herb, pulverize it through an 80-mesh sieve, place it in an extraction tank, add 15 times the weight of the herb in deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 40℃, and the extraction time is 90 min. Separate the extract using a plate centrifuge, collect the extract, add 1 times the weight of the herb in D301 macroporous weak base anion exchange resin for decolorization, stir and adsorb for 3 hours, then perform solid-liquid separation. Separate and concentrate the decolorized solution using a 20kD ultrafiltration membrane, and dry the concentrate under reduced pressure to obtain tuberose polysaccharide. Step 2: Crush Scutellaria baicalensis, Centella asiatica and chamomile into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 3: After pulverizing the compound medicinal materials obtained in Step 2, place them in an extraction tank, add 20% of the compound medicinal materials' mass of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 4: Place the tuberose polysaccharide from Step 1 and the compound extract from Step 3 into an extraction vessel, stir and dissolve them, then add 30% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition. Comparative Example 8

[0044] A method for preparing a plant-based composition with soothing effects for use in cosmetics, comprising the following steps: Step 1: Crush Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; Step 2: After pulverizing the compound medicinal materials obtained in Step 1, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated using a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.010, and the obtained concentrated liquid is the compound extract; Step 3: Place the compound extract from Step 2 into an extraction vessel, stir and dissolve it, then add 30% by weight of butylene glycol as an extractant to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition.

[0045] The preparation methods of the plant compositions in Comparative Examples 9 and 10 are the same as those in Example 1.

[0046] Take the plant compositions obtained in Examples 1-7 and Comparative Examples 1-10, and prepare the corresponding soothing essences according to the formulations in Table 2.

[0047] Table 2

[0048] The soothing serum is prepared as follows: Thoroughly stir phase A until well mixed. While stirring, slowly add the pre-dissolved group B to phase A and continue stirring for 30 minutes. Cool to room temperature to obtain the soothing serum.

[0049] The soothing serum products of each embodiment and comparative example were prepared using the same method, and efficacy tests were conducted on inhibiting inflammation, releasing allergic mediators, repairing the skin barrier, reducing redness and swelling, and soothing the skin, as well as safety tests.

[0050] Comparative experiment on the inhibitory effect of plant compositions on hyaluronidase

[0051] Hyaluronidase is involved in allergic reactions and is strongly correlated with inflammation and allergies. Many anti-allergy drugs work by inhibiting hyaluronidase activity. Therefore, studying the inhibition of hyaluronidase activity can serve as an indicator of the anti-allergic effect of cosmetics.

[0052] This experiment employed the modified Elson-Morgan method: hyaluronidase hydrolyzes hyaluronic acid to produce β-N-acetylglucosamine, which then condenses with acetylacetone under alkaline conditions to form a 2-methyl-3-diacetylpyrrole derivative. This 2-methyl-3-diacetylpyrrole derivative reacts with p-dimethylaminobenzaldehyde in concentrated hydrochloric acid-ethanol solution. The reactants exhibit characteristic absorption at 530 nm, and the change in absorbance is linearly correlated with the degree of hyaluronidase inhibition. By measuring the change in absorbance, the hyaluronidase inhibition rate can be calculated, thereby analyzing the anti-allergic activity of the cosmetic.

[0053] Take 0.1 mL of 0.25 mmol / L CaCl2 solution and 0.5 mL of hyaluronidase solution, and incubate at 37°C for 20 min. Add 0.5 mL of soothing essence solution and continue incubation at 37°C for 20 min. Add 0.5 mL of sodium hyaluronate solution and incubate at 37°C for 30 min, then let stand at room temperature for 5 min. Add 0.1 mL of 0.4 mol / L NaOH solution and 0.5 mL of acetylacetone solution, heat in a boiling water bath for 15 min, and immediately cool with ice water for 5 min. Add 1.0 mL of Ehrlich reagent and dilute with 3.0 mL of anhydrous ethanol, let stand for 20 min for color development, and measure its absorbance value (ABS value) with a spectrophotometer. The formula for calculating the hyaluronidase inhibition rate is as follows.

[0054]

[0055] In the formula: A is the ABS value of the control solution, B is the ABS value of the control blank solution, C is the ABS value of the sample solution, and D is the ABS value of the sample blank solution.

[0056] During the experiment, the wavelength of the sample in Example 1 was first scanned in the range of 450 to 700 nm to determine the maximum absorption wavelength. Then, using deionized water as a reference, the ABS values ​​of all comparative examples were measured at the maximum absorption wavelength.

[0057] Table 3 shows a comparison of the hyaluronidase inhibition effects of Examples 1-7 and Comparative Examples 1-10.

[0058] Table 3 Example 1 73.2% Comparative Example 3 31.1% Example 2 69.7% Comparative Example 4 30.5% Example 3 68.1% Comparative Example 5 35.6% Example 4 72.9% Comparative Example 6 33.4% Example 5 71.6% Comparative Example 7 37.3% Example 6 56.2% Comparative Example 8 39.6% Example 7 55.3% Comparative Example 9 45.7% Comparative Example 1 20.5% Comparative Example 10 43.4% Comparative Example 2 25.8% / / As can be seen from Table 3, compared with the comparative examples, under the same addition concentration conditions, the hyaluronidase inhibition rate of Examples 1-7 was significantly higher than that of Comparative Examples 1-10, indicating that the plant composition of the present invention has a significant anti-allergic effect.

[0059] As can be seen from the test results of Examples 6 and 7, when the ratio of the raw materials of the active ingredient is within the range of the present invention, but the preparation process parameters are not within the range of the present invention, the hyaluronidase inhibition rate will decrease significantly, but it is still better than the comparative example.

[0060] The test results of Comparative Examples 1 to 10 show that when the raw materials of some active ingredients in this patent are removed or replaced with traditional Chinese medicines with similar functions, the hyaluronidase inhibition rate will decrease significantly.

[0061] The results of the study on hyaluronidase inhibition rate show that the plant composition of the present invention has a significant hyaluronidase inhibition ability, a clear synergistic effect, and exhibits a good anti-allergic effect.

[0062] Inhibitory effect of plant composition on inflammatory factor IL-6

[0063] Interleukin-6 (IL-6) is a cytokine belonging to the interleukin family. It is produced by fibroblasts, monocytes / macrophages, T / B lymphocytes, epithelial cells, keratinocytes, and various tumor cells. IL-6 can stimulate the proliferation and differentiation of cells involved in immune responses and enhance their function. It also plays a significant role in febrile and inflammatory responses, serving as an important inflammatory mediator.

[0064] The experimental method for the inhibitory effect of plant composition on the inflammatory factor IL-6 is as follows: RAW264.7 cell suspension was taken and the density was controlled at 5 × 10⁻⁶. 5 Cells were seeded at approximately 100 cells / mL using 96-well plates, with 200 μL of cell suspension per well. The cells were divided into a blank group, a positive group, and a drug-treated group. 0.4 μL of DMSO was added to each well of the blank group; 0.4 μL of DMSO and 2 μL of LPS were added to each well of the positive group; and 2 μL of LPS and 0.4 μL of the soothing essence from the examples and comparative examples were added to each well of the drug-treated group. Each well was incubated at 37℃ for 2 hours, washed three times with DPBS, patted dry, and then 100 μL of enzyme conjugation working solution (except for blank wells), 100 μL of chromogenic solution, and 50 μL of stop solution were added sequentially. After thorough mixing for 5 minutes, the OD value was measured, a standard curve was plotted, and the concentration of IL-6 was calculated. The experimental results are shown in Table 4.

[0065] Table 4 Blank group 215±22 Comparative Example 1 1147±42 positive group 1687±143 Comparative Example 2 1256±48 Example 1 325±34 Comparative Example 3 1025±47 Example 2 336±28 Comparative Example 4 976±39 Example 3 328±39 Comparative Example 5 836±41 Example 4 320±35 Comparative Example 6 857±37 Example 5 332±43 Comparative Example 7 572±36 Example 6 416±41 Comparative Example 8 586±34 Example 7 405±45 Comparative Example 9 603±33 / / Comparative Example 10 617±37 Table 4 shows the results of the study on the inhibition of the inflammatory factor IL-6. After stimulation with 1 μg / ml LPS, the cells released IL-6 at a concentration of 1687±143 pg / mL, which was significantly different from the control group (215±22 pg / mL) (P<0.05). This result indicates that LPS can significantly induce an inflammatory response in RAW264.7 cells and generate IL-6.

[0066] Compared with the comparative examples, under the same concentration conditions, the IL-6 inhibitory effect of Examples 1-7 was significantly higher than that of Comparative Examples 1-10, indicating that the plant composition of the present invention has a significant anti-inflammatory effect.

[0067] As can be seen from the test results of Examples 6 and 7, when the ratio of the raw materials of the active ingredient is within the range of the present invention, but the preparation process parameters are not within the range of the present invention, its IL-6 inhibitory effect will decrease significantly, but it is still better than the comparative example.

[0068] The test results of Comparative Examples 1 to 10 show that when the raw materials of some active ingredients in this patent are removed or replaced with traditional Chinese medicines with similar functions, the inhibitory effect of IL-6 is significantly reduced.

[0069] The results of the study on the inhibitory effect of IL-6 show that the plant composition of the present invention has a significant inhibitory effect on IL-6, has a clear synergistic effect, and shows a good anti-inflammatory effect.

[0070] Free radical scavenging ability of plant compositions

[0071] Free radicals generated by mitochondria during electron transport can attack cell membranes, proteins, and even DNA, leading to cell aging and even death, thus causing allergic reactions in the human body. Inhibiting free radical activity can effectively alleviate skin allergy symptoms caused by external stimuli, thereby maintaining the normal barrier structure of the skin. Evaluating the free radical scavenging ability of plant compositions can, to some extent, reflect their ability to repair the skin barrier.

[0072] The evaluation method for the scavenging ability of plant compositions against DPPH free radicals is as follows: (1) Preparation of DPPH working solution: Weigh 5.0 mg of DPPH (1,1-diphenyl-2-trimethyl-picryl-2-amidinyl), dissolve in an appropriate amount of anhydrous ethanol, sonicate in the dark to ensure complete dissolution, and then dilute to 100.0 mL with anhydrous ethanol to prepare a DPPH working solution of 50.0 ug / mL. (2) Preparation of test solution: Weigh 5 g each of the plant compositions obtained in Examples 1-7 and Comparative Examples 1-10, and dilute to 100.0 mL with deionized water. (3) Add 2 ml of test solution and 2 ml of DPPH working solution to the same stoppered test tube, shake well, and measure its absorbance Ai at 517 nm after 30 min. At the same time, measure the absorbance A0 after mixing 2 ml of DPPH solution with 2 ml of 80% ethanol. The scavenging ability of the plant compositions in the examples and comparative examples against DPPH free radicals is calculated by the following formula:

[0073] The experimental results of the scavenging ability of the plant compositions obtained in Examples 1-7 and Comparative Examples 1-10 against DPPH free radicals are shown in Table 5.

[0074] Table 5 Example 1 85.6% Comparative Example 3 33.8% Example 2 80.2% Comparative Example 4 37.4% Example 3 81.9% Comparative Example 5 41.3% Example 4 83.7% Comparative Example 6 43.5% Example 5 85.1% Comparative Example 7 56.4% Example 6 72.3% Comparative Example 8 57.9% Example 7 75.6% Comparative Example 9 63.5% Comparative Example 1 25.7% Comparative Example 10 61.5% Comparative Example 2 21.6% / / As can be seen from Table 5, compared with the comparative examples, under the same addition concentration conditions, the DPPH free radical scavenging rate of Examples 1-7 was significantly higher than that of Comparative Examples 1-10, indicating that the plant composition of the present invention has a significant DPPH free radical scavenging ability.

[0075] The test results of Examples 6 and 7 show that when the ratio of the raw materials of the active ingredient is within the range of the present invention, but the preparation process parameters are not within the range of the present invention, its DPPH free radical scavenging ability will decrease significantly, but it is still better than the comparative example.

[0076] The test results of Comparative Examples 1 to 10 show that when the raw materials of some active ingredients in this patent are removed or replaced with plants with similar functions, its DPPH free radical scavenging ability will decrease significantly.

[0077] The results of the study on the DPPH free radical scavenging capacity show that the plant composition of the present invention has a significant DPPH free radical scavenging ability, a clear synergistic effect, and a good barrier repair effect.

[0078] Skin barrier repair and soothing effects of plant-based compositions tested

[0079] Transdermal water loss (TEWL) refers to the amount of water transported through the skin via passive diffusion, reflecting the health of the skin barrier. Generally, a low TEWL value indicates good skin barrier function. However, sensitive skin often has some damage to its skin barrier function, resulting in a higher TEWL. Therefore, changes in TEWL can be used to reflect changes in skin barrier function. When soothing agents are used on sensitive skin, the TEWL value can be measured using the Tewameter TM300 skin moisture loss test probe. A lower TEWL value indicates less water loss through the skin, better skin barrier function, and a better repairing effect of the corresponding soothing product on the skin barrier. Therefore, changes in TWL values ​​can be used to determine the skin barrier repair effect of plant-based compositions.

[0080] Skin hemoglobin reflects the condition of the skin's blood vessels, indicating inflammation and the integrity of the skin barrier. When skin is allergic, higher hemoglobin levels indicate more severe redness and more intense inflammation. Therefore, after using soothing agents on allergic skin, changes in skin hemoglobin levels can be measured using the Mexameter MX18 hemoglobin testing probe to assess the soothing and repairing effects of the plant-based composition on allergy symptoms.

[0081] (1) Subjects: 102 subjects were selected, 51 males and 51 females, aged 20-45 years. Subjects had no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, no history of allergies to skin care cosmetics, and had not used systemic hormone drugs or immunosuppressants within the past month. Subjects were randomly divided into 17 groups, with 6 subjects in each group, 3 males and 3 females. The soothing essence prepared using the above-described examples and comparative plant compositions was used in a comparative experiment to evaluate its soothing effects.

[0082] (2) Test method: On day 0 of the experiment, the subjects sat quietly for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 40~60%). Two circular areas with a diameter of 18mm were marked on the corresponding positions on the inner side of the left forearm, with a 3cm interval between the two areas. Erythema was induced in the marked areas on the inner side of the left forearm by a closed patch test with 2% SLS solution for 24 hours. After 24 hours of patch application, the patch applicator was removed from the arm, and 2ml of the soothing essence prepared by the above-mentioned plant composition of the example and comparative example was evenly applied to the patch area, once in the morning and once in the evening. After 48 hours, the skin parameters, average transepidermal water loss (TEWL value) and skin hemoglobin content of the patch area of ​​the blank group and the experimental group were measured by the Tewameter™ 300 skin moisture loss test probe and the Mexameter MX18 hemoglobin test probe, respectively.

[0083] The results of the skin barrier repair and soothing effects tests in the examples and comparative examples are shown in Table 6.

[0084] Table 6

[0085] As can be seen from the test results of transdermal water loss in Table 6: (1) After 48 hours of use of the soothing essence by the subjects, the average change in transepidermal water loss in Examples 1-7 was 3.95 g / m³. 2 After h, the skin barrier essentially returned to normal levels. The average change in transepidermal water loss in comparative examples 1–10 was 21.07 g / m². 2 h indicates that the plant composition product of the present invention has a significant effect on repairing the skin barrier.

[0086] (2) The test results of Examples 6-7 show that when the ratio of the raw materials of the active ingredient is within the range of the present invention, but the preparation process parameters are not within the range of the present invention, its skin barrier repair ability is significantly reduced and there is still a large gap from the normal skin barrier function, but it is still better than the comparative example.

[0087] (3) As can be seen from the test results of comparative examples 1 to 10, when the raw materials of some active ingredients in this patent are deleted or replaced with traditional Chinese medicine with similar functions, the skin barrier repair ability will be greatly reduced. In some cases, the test results of individual comparative examples are close to those of the blank group, and there is still a large gap from the normal skin barrier function.

[0088] The experimental results of skin red pigment content in Table 6 show that: (1) The test results of Examples 1 to 5 showed that after the subjects used the soothing essence for 48 hours, the skin red pigment content basically returned to normal level, and the results were all significantly different (P<0.05), indicating that the plant composition product of the present invention has a good effect on improving skin erythema and relieving skin inflammation.

[0089] (2) The test results of Examples 6-7 show that when the ratio of the raw materials of the active ingredient is within the range of the present invention, but the preparation process parameters are not within the range of the present invention, its effect on improving skin erythema and relieving skin inflammation is significantly weakened, but it is still better than the comparative example.

[0090] (4) The results of the soothing test of Comparative Examples 1 to 10 show that when only one or two functional raw materials are used, or when other Chinese medicinal materials with similar functions are used, the effect on improving skin erythema and relieving skin inflammation is not obvious, and there is still a large gap from the normal skin hemoglobin content.

[0091] As can be seen from the test results in Table 6, the plant composition of the present invention has good effects in relieving skin allergy symptoms and repairing the skin barrier, and the active ingredients have a clear synergistic effect.

[0092] Test on the efficacy of plant-based compositions in relieving itching and redness

[0093] Histamine is an autoactive substance that plays an important role in the regulation of allergies and inflammation. When mast cells degranulate and release histamine, an allergic mediator, it usually stimulates surrounding soft tissues and peripheral nerves, leading to dilation of peripheral capillaries and the formation of edema, or the production of inflammatory erythema and the induction of itching. Therefore, the soothing efficacy of different plant compositions can be evaluated by relieving histamine-induced itching and swelling in humans.

[0094] (1) Subjects: Eighteen qualified subjects were selected, nine males and nine females, aged 20-45 years. Subjects had no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, no history of allergy to skin care cosmetics, and had not used systemic hormone drugs or immunosuppressants within one month. Subjects were randomly divided into three groups: experimental group, control group, and positive group, with six subjects in each group, three males and three females. The soothing essence prepared using the plant composition of Example 5 and Comparative Example 5 was tested for its efficacy in relieving itching and redness.

[0095] (2) Test method: Subjects sat quietly in a constant temperature and humidity environment for 30 minutes, with a temperature of 21±1℃ and a humidity of 40~60%. A mark was made approximately 4cm inside the subject's arm. 2 For each area, 20 μL of 2% histamine solution was applied to the marked area and spread evenly. Observation was performed; noticeable redness and swelling within 20 minutes indicated a qualified subject. Six subjects in each group had histamine solution applied to their arms. After itching and slight redness and swelling occurred, the test substance was applied to the affected area for testing. The test substance for the positive group was a soothing essence containing no other ingredients; the test substance for the experimental group was a soothing essence containing the plant composition of Example 5; and the test substance for the control group was a soothing essence prepared with the plant composition of Comparative Example 5. The average time for relief and disappearance of itching, as well as the average time for elimination of redness and swelling, were recorded. The results are shown in Table 7 below. Skin symptoms were photographed before and 60 minutes after application of the test substance. Figure 1 .

[0096] Table 7 experimental group 8 42 63 control group 27 85 145 positive group 56 138 225 From Table 7 and Figure 1 The test results show that, compared with the control group and the positive group, the plant composition of the present invention can relieve itching and discomfort in a shorter time and can eliminate the redness and swelling caused by histamine in about 60 minutes. The plant composition of the present invention has a good effect in relieving itching and eliminating redness and swelling.

[0097] Safety testing of plant-based compositions

[0098] The chorioallantoic membrane (CAM) is a respiratory membrane surrounding the chicken embryo. Because the surface of the CAM is richly vascularized, it can be considered a complete organism. By observing the effects of substances on the chicken embryo's vascular system, the effects of those substances on the human body can be predicted. In cosmetic safety evaluation, this test can be used to detect and assess the vascular toxicity, skin irritation, and eye irritation of cosmetic ingredients.

[0099] In this experiment, taking advantage of the characteristics of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the mid-term of hatched chicken embryos, 0.3 g of the test substance was directly contacted with the allantoic membrane of the chicken embryos. After acting for 5 minutes, the changes in the toxicity effect indicators of the chorioallantoic membrane (bleeding, coagulation, and vascular lysis) were observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation in the chorioallantoic membrane and its degree of damage. Then, a score was combined for safety assessment. The average ES score = the sum of the degrees of bleeding, coagulation, and vascular lysis observed in 6 chicken embryos divided by 3. When the ES value ≤ 12, it is non / lightly irritating; when 12 < ES value < 16, it is moderately irritating; when the ES value ≥ 16, it is severely irritating. The evaluation criteria are shown in Table 8.

[0100] Table 8 0 No bleeding No clotting Angiolysis 1 Mild bleeding Mild coagulation Mild angiogenesis 2 Moderate bleeding Moderate coagulation moderate vessel dissolution 3 Severe bleeding Severe coagulation severe vasolysis The negative control group was 0.9% NaCl solution, the positive control group was 0.5% SDS solution, and the experimental group was an aqueous deionized solution containing 20% of the plant composition of Example 1. The test results are shown in Table 9 and Figure 2 as follows.

[0101] Table 9 negative group 1.3 Non-irritating positive group 18.5 Severe stimulation experimental group 2.2 Non-irritating From the safety test results of the plant composition, it can be seen that the reaction results of the negative control and positive control in the irritation experiment are exactly within the classification ranges of non-irritating and strongly irritating, respectively, indicating that the test results are acceptable.

[0102] The plant composition of the present invention can well maintain the morphological structure, color, and permeability of blood vessels and vascular networks, and has good safety.

[0103] The above is a further detailed description of the present invention, and it should not be regarded as a limitation to the specific implementation of the present invention. For those of ordinary skill in the technical field to which the present invention pertains, simple deductions or substitutions without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A plant-based composition for use in cosmetics, characterized in that, The active ingredients are: tuberose, scutellaria baicalensis, centella asiatica, chamomile, and sage. The mass ratio of the active ingredients is: 15 parts tuberose, 4 parts scutellaria baicalensis, 4 parts centella asiatica, 4 parts chamomile, and 4 parts sage. The preparation method of the plant composition includes the following steps: 1) Weigh the tuberose medicinal material, pulverize it through an 80-mesh sieve, place it in an extraction tank, add 15 times the weight of the medicinal material of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 40℃, and the extraction time is 90 min; the extracted material is separated by a plate centrifuge, the extract is collected, and 1 times the weight of the medicinal material of D301 macroporous weak base anion exchange resin is added for decolorization, the mixture is stirred and adsorbed for 3 hours, solid-liquid separation is performed, the decolorized liquid is separated and concentrated by an ultrafiltration membrane with a pore size of 20 kD, and the concentrated liquid is dried under reduced pressure to obtain tuberose polysaccharide; 2) Grind Scutellaria baicalensis, Centella asiatica, chamomile, and Sage into powder, mix the powdered herbs evenly to obtain compound herbal powder; 3) After pulverizing the compound medicinal materials obtained in step 2, place them in an extraction tank, add 20 times the weight of deionized water for extraction, the extraction speed is 80 rpm, the extraction temperature is 45℃, and the extraction time is 120 min; the extracted materials are separated by a plate centrifuge, the obtained extract is filtered through a ceramic membrane with a pore size of 500 nm, the obtained filtrate is purified through an ultrafiltration membrane with a pore size of 5 kD, and the purified liquid is concentrated through a nanofiltration membrane with a pore size of 100 Da. Concentration is stopped when the specific gravity of the concentrated liquid is 1.

010. The obtained concentrated liquid is the compound extract. 4) Place the tuberose polysaccharide from step 1 and the compound extract from step 3 into an extraction vessel, stir and dissolve them, then add 30% by weight of the extractant butylene glycol to the solution, stir and mix evenly, let stand for 15 hours, and take the supernatant to obtain the plant composition.

2. A skincare product with soothing effects, comprising excipients and active ingredients, characterized in that, The active ingredient is the plant composition according to claim 1.

3. The skincare product according to claim 2, characterized in that, The plant composition is added at a rate of 0.1% to 10% by mass.

4. The skincare product according to claim 3, characterized in that, The skincare products mentioned include ointments, creams, liquids, aerosols, lotions, gels, shampoos, shower gels, and facial cleansers.

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