Herbal composition, method of making and use thereof

Abstract

The application discloses a kind of herbal compositions and its preparation method and application, belong to cosmetic technical field;The raw materials of herbal composition provided in the application cooperate with each other, can realize the effect of excellent relief skin sensitive, can also achieve excellent whitening effect simultaneously;It can be added as component in the preparation of skin care product to realize the effect of whitening and soothing.And the preparation method of herbal composition provided in the application is high pressure difference extraction, can better avoid the structure damage and the reduction of efficacy caused by conventional reflux extraction of effective component, so as to realize more excellent comprehensive effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to an herbal composition, its preparation method, and its application. Background Technology

[0002] Skin whitening is always a top concern for Asians, especially Asian women, and it's also a fundamental requirement for modern functional cosmetics and skincare products. Human skin color is primarily determined by the amount of melanin, a dark brown pigment found in animal skin and hair. Melanin production is related to endocrine disorders, sun exposure, and environmental pollution. Factors such as ultraviolet radiation, genetics, inflammatory factors, improper diet, work stress, and irregular lifestyles can lead to abnormal melanin metabolism, resulting in excessively rapid melanin production and uneven distribution, causing localized darkening and pigmentation.

[0003] The factors leading to sensitive skin are complex, but looking at the trends over the past 10-20 years, the main causes are improved living standards and a pursuit of beauty, leading to minimally invasive procedures on the skin, such as micro-surgery, microneedling, laser treatments, Thermage, and injectable moisturizing and anti-aging products. The fast pace of modern life, stress from work and life, and environmental pollution also contribute to skin sensitivity, especially among urban women, with over 80% experiencing sensitive skin. A common characteristic of sensitive skin is redness, swelling, and itching, which is difficult to heal. However, because the underlying sensitive skin problem remains unresolved, consumers cannot afford to pursue skin whitening or other skin-beautifying effects.

[0004] Patent CN114886811A discloses a composition for repairing sensitive skin, comprising extracts of *Vigna oleifera*, *Portulaca oleracea*, *Chamomile*, *Aesculus hippocastanum*, ergothioneine, *Pine mushroom*, hyaluronic acid, β-glucan, *Tremella fuciformis* polysaccharide, vitamin B5, *Okra* fruit extract, yeast lysate extract, lecithin, ceramide, polyglutamic acid, and oligopeptide-2. This composition targets the repair of sensitive skin; however, it does not simultaneously repair sensitive skin and whiten the skin. Therefore, there is an urgent need to develop a skincare product that can both address skin sensitivity and whiten the skin to meet market demand. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a herbal composition suitable for sensitive skin, capable of relieving skin sensitivity and having excellent whitening effects, as well as its preparation method and application.

[0006] To achieve the above objectives, in a first aspect of the present invention, the present invention provides an herbal composition comprising the following raw materials: *Hypericum gloriosa*, citron, purslane, chamomile, *Astragalus membranaceus* root, cherry blossom, *Polygonum cuspidatum*, cornus officinalis, licorice, and ergothioneine.

[0007] The ingredients in the herbal composition provided by this invention work together to achieve excellent relief of skin sensitivity, while also achieving excellent whitening effects. Among the herbal ingredients, the healing velvet flower, citron, purslane, chamomile, and astragalus root have a calming, soothing, antibacterial, and anti-irritant effect on the skin. They can effectively inhibit the production of inflammatory factors in adipocytes and histamine signaling pathways caused by necrosis factor, promote the production of β-endorphin, thereby strengthening the skin barrier repair and providing more nourishing nutrients, thus achieving the effects of anti-oxidation and repairing sensitive skin. The cherry blossom, ergothioneine, and cornus officinalis in the herbal ingredients can jointly inhibit the activity of tyrosinase in the skin, thereby reducing the production of melanin and achieving the effect of whitening and fading spots. At the same time, polygonum cuspidatum and licorice also have a certain whitening effect. Polygonum cuspidatum and licorice can simultaneously improve the skin's microcirculation, making the content of oxygenated hemoglobin in the blood higher and bright red. This not only makes the skin appear delicate and rosy, but also enhances skin metabolism to improve skin transparency and dullness, thus achieving the effect of rosy, shiny, delicate, and white skin.

[0008] Specifically, *Healing Velvet Flower* refers to the petals, which are safe and effective, possessing skin-care, spasm-relieving, detoxifying, and swelling-reducing properties. It can promote the healing of damaged facial skin and soothe sensitive skin. Citron refers to the fruit, which has excellent antioxidant properties. Purslane refers to the leaves and stems, which have excellent anti-dryness and anti-aging effects, increase skin comfort, and scavenge free radicals, improving skin comfort. Chamomile refers to the petals, which have excellent calming and soothing effects. Astragalus membranaceus root refers to the root, which has anti-inflammatory properties. It has antioxidant, anti-inflammatory, immune-boosting, anti-aging, and collagen-promoting effects, providing comprehensive protection and repair for the skin; cherry blossom refers to the petals, which promote the activity of aromatase, have a broad-spectrum scavenging effect on various free radicals, and also inhibit the activity of tyrosinase; dogwood refers to the fruit, which has antibacterial, anti-inflammatory, antioxidant, anti-aging, and whitening effects; Japanese knotweed refers to the rhizome, which has antibacterial and tyrosinase-inhibiting effects; licorice refers to the rhizome, which has whitening, antibacterial, and anti-inflammatory effects.

[0009] As a preferred embodiment of the herbal composition of the present invention, the herbal composition comprises the following raw materials in parts by weight: 5-30 parts of *Hypericum gloriosa*, 5-30 parts of *Citrus medica*, 5-30 parts of *Portulaca oleracea*, 5-30 parts of *Chamomile*, 5-30 parts of *Astragalus membranaceus* root, 10-50 parts of cherry blossom, 10-50 parts of *Polygonum cuspidatum*, 10-50 parts of *Cornus officinalis*, 10-50 parts of licorice, and 5-20 parts of ergothioneine.

[0010] Preferably, the herbal composition comprises the following raw materials in parts by weight: 10-20 parts of *Hypericum gloriosa*, 10-20 parts of *Citrus medica*, 10-20 parts of *Portulaca oleracea*, 10-20 parts of *Chamomile*, 10-20 parts of *Astragalus membranaceus* root, 20-30 parts of cherry blossom, 20-30 parts of *Polygonum cuspidatum*, 20-30 parts of *Cornus officinalis*, 20-30 parts of licorice, and 10-15 parts of ergothioneine.

[0011] More preferably, the herbal composition comprises the following raw materials in parts by weight: 15 parts of *Hypericum gloriosa*, 15 parts of citron, 15 parts of purslane, 15 parts of chamomile, 15 parts of *Astragalus membranaceus* root, 25 parts of cherry blossom, 25 parts of Polygonum cuspidatum, 25 parts of Cornus officinalis, 25 parts of licorice, and 13 parts of ergothioneine.

[0012] The present invention has found that the weight proportions of raw materials in the herbal composition affect the overall effect of the product. When the weight proportions of raw materials in the herbal composition are further selected within the above-mentioned range, the overall effect of the product is even better; specifically, the whitening effect and antioxidant effect are better.

[0013] As a preferred embodiment of the herbal composition of the present invention, the weight ratio of citron, Astragalus membranaceus root, cherry blossom, and Cornus officinalis is citron: Astragalus membranaceus root: cherry blossom: Cornus officinalis = 1:(0.8-1.2):(1.2-2):(1.2-2).

[0014] This invention has found that the weight ratio of citron, astragalus root, cherry blossom, and cornus officinalis in the herbal composition raw materials also affects the performance of the product. When the weight ratio of citron, astragalus root, cherry blossom, and cornus officinalis is citron: astragalus root: cherry blossom: cornus officinalis = 1:(0.8-1.2):(1.2-2):(1.2-2), the overall effect of the obtained product is better.

[0015] As a preferred embodiment of the herbal composition of the present invention, the herbal composition further includes the following raw materials: Sophora japonica fruit, Sichuan pepper, Forsythia suspensa, and Chrysanthemum morifolium.

[0016] This invention has found that further addition of Sophora japonica fruit, Sichuan pepper, Forsythia suspensa, and chamomile to the herbal composition can achieve better whitening and soothing effects. Specifically, Sophora japonica fruit refers to the fruit of the Sophora japonica tree, which has anti-inflammatory and skin-soothing effects; Sichuan pepper refers to the fruit, which has blood-activating, stasis-removing, anti-inflammatory, and analgesic effects; Forsythia suspensa refers to the fruit, which has heat-clearing, detoxifying, nodule-dispersing, and carbuncle-reducing effects; and chamomile refers to the petals, which can effectively soothe the skin, resist skin sensitivity, and has anti-inflammatory, antibacterial, and antioxidant effects.

[0017] As a preferred embodiment of the herbal composition of the present invention, the herbal composition further includes the following raw materials in parts by weight: 5-30 parts of Sophora japonica fruit, 5-30 parts of Zanthoxylum bungeanum, 5-20 parts of Forsythia suspensa, and 10-50 parts of Chrysanthemum morifolium.

[0018] Preferably, the herbal composition further includes the following raw materials in parts by weight: 10-20 parts of Sophora japonica fruit, 10-20 parts of Zanthoxylum bungeanum, 10-15 parts of Forsythia suspensa, and 20-30 parts of Chrysanthemum morifolium.

[0019] More preferably, the herbal composition further includes the following raw materials in parts by weight: 15 parts Sophora japonica fruit, 15 parts Zanthoxylum bungeanum, 13 parts Forsythia suspensa, and 25 parts Chrysanthemum morifolium.

[0020] The present invention has found that the weight percentages of Sophora japonica fruit, Sichuan pepper, Forsythia suspensa, and chrysanthemum also affect the performance of the product. Further selection of the above-mentioned raw materials by weight percentage, especially further optimization of the raw materials by weight percentage, results in a product with better overall performance.

[0021] In a second aspect, the present invention provides a method for preparing the herbal composition, the method comprising the following steps:

[0022] (1) After crushing the wound-healing velvet flower, mix it with water, then soak it under pressure. After soaking for a period of time, extract it under pressure. After extraction, filter it and collect the filtrate for enzymatic hydrolysis. After enzymatic hydrolysis, extract A is obtained.

[0023] (2) The remaining raw materials, except for ergothioneine, are crushed and mixed with water. Then, they are soaked under pressure. After soaking for a period of time, they are extracted under pressure. After extraction, they are filtered and the filtrate is collected to obtain extract B.

[0024] (3) Mix and concentrate extract A, extract B and ergothioneine to obtain herbal composition.

[0025] The method for preparing the herbal composition provided by the present invention involves first soaking and enzymatically hydrolyzing the wound-healing velvet flower under pressure, then soaking the remaining components under pressure, and finally mixing the soaked products. This method can effectively preserve the active ingredients in the raw materials, thereby helping to achieve the whitening and soothing effects of the herbal composition.

[0026] As a preferred embodiment of the preparation method of the present invention, at least one of the following (a)-(c) is used:

[0027] (a) In the pressurized extraction, the pressure change is to increase the pressure from 0.2 MPa to more than 70 MPa within 0.5-1 s;

[0028] (b) The soaking time is 60-120 min, and the solid-liquid ratio is 10-30.

[0029] (c) The enzyme used in the enzymatic hydrolysis is a restriction endonuclease;

[0030] (d) The temperature of the water is 15-30℃.

[0031] Preferably, in the pressurized extraction, the pressure change is to increase the pressure from 0.2MPa to 70-90MPa within 0.5-1s.

[0032] More preferably, in the pressurized extraction, the pressure change is to increase the pressure from 0.2 MPa to 90 MPa within 0.5-1 s.

[0033] This invention employs a high-pressure differential extraction process, rapidly increasing the pressure to a certain value within a short time. The resulting powerful mechanical force creates a liquid microjet velocity; the greater the pressure difference, the greater the microjet velocity. This causes high-speed pressure collisions between material cells, leading to cell wall rupture and extraction of the original fluid within the cells. This allows for a greater extraction of the effective functional components from each raw material. Compared to traditional extraction methods, this method more effectively avoids changes in the structure and loss of functional components due to high temperatures, as well as a reduction in physiological activity, thus ensuring the product's efficacy. Further increasing the pressure from 0.2 MPa to 90 MPa achieves excellent overall product performance while avoiding even higher pressures.

[0034] Preferably, the enzyme is Hind III enzyme.

[0035] This invention employs enzymatic digestion technology to treat *Vallisneria natans*, primarily using directional enzymatic digestion. This involves using restriction endonucleases to cut the DNA fragments of tannins and polyphenols from *Vallisneria natans*, and applying Hind III enzymes to cut the DNA molecules at recognition sites, followed by dissociation from the substrate. This process increases the purity and concentration of tannins and polyphenols. The resulting extract A contains a tannin concentration greater than 0.23% and a polyphenol concentration greater than 0.32%.

[0036] Preferably, the enzymatic hydrolysis time is 140-160 min and the enzymatic hydrolysis temperature is 20-25℃.

[0037] Preferably, the enzymatic hydrolysis further includes an enzyme inactivation treatment, specifically: incubation at 80-90℃ for 15-25 minutes.

[0038] Preferably, the pulverized raw material has a mesh size of 200-250.

[0039] Preferably, the filtration is a ceramic membrane.

[0040] Preferably, the concentration is performed using a reverse osmosis membrane.

[0041] In a third aspect, the present invention provides the use of the herbal composition in the preparation of skin care products.

[0042] In a fourth aspect, the present invention provides a skin care product comprising the herbal composition described herein.

[0043] As a preferred embodiment of the skin care product of the present invention, the herbal composition comprises 0.5-30% by mass, based on the skin care product.

[0044] The present invention has found that when the mass percentage of the herbal composition is further selected within the above-mentioned range, excellent overall effects can be achieved while avoiding waste of raw materials.

[0045] As a preferred embodiment of the skincare product described in this invention, the skincare product includes at least one of the following: essence mask, essence liquid, essence lotion, essence cream, essence eye cream, and essence water.

[0046] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0047] The herbal composition provided by this invention exhibits synergistic effects, effectively relieving skin sensitivity and achieving excellent whitening results. It can be added as a component to skincare products to achieve whitening and soothing effects. Furthermore, the preparation method of the herbal composition provided by this invention is high-pressure differential extraction, which better avoids structural damage and efficacy reduction of active ingredients caused by conventional reflux extraction, thereby achieving a superior overall effect. Attached Figure Description

[0048] Figure 1 The before-and-after comparison image for volunteer 1 in Example 2 shows the effect.

[0049] Figure 2 The before-and-after comparison image for volunteer 2 in Example 2 shows the effect.

[0050] Figure 3 The before-and-after comparison chart for volunteer 3 in Example 2 shows the effect.

[0051] Figure 4 The before-and-after comparison chart for volunteer four in Example 2 shows the effect.

[0052] Figure 5 The before-and-after comparison chart for volunteer five in Example 2 shows the effect.

[0053] Figure 6 The before-and-after comparison chart for volunteer six in Example 2 shows the effect.

[0054] Figure 7 This is a before-and-after comparison image of volunteer 1 in the control group in Example 2;

[0055] Figure 8 This is a before-and-after comparison chart of volunteer 2 in the control group in Example 2;

[0056] Figure 9 The image shows the results of moisture retention on the right cheek after applying the mask in Example 3.

[0057] Figure 10 The image shows the results of moisturizing the skin on the left side of the face after applying the mask in Example 3. Detailed Implementation

[0058] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0059] Unless otherwise specified, the reagents, methods and equipment used in this invention are all conventional reagents, methods and equipment in the field.

[0060] Examples 1-7 and Comparative Examples 1-5

[0061] Examples 1-7 and Comparative Examples 1-5 of the present invention provide a herbal composition, the raw materials (parts by mass) of the herbal composition are shown in Table 1;

[0062] Table 1

[0063]

[0064]

[0065] The method for preparing the herbal composition provided in Example 1 includes the following steps:

[0066] (1) Pulp the wound-healing velvet flower into 200 mesh and mix it with 20 times its weight of water (temperature 20±5℃). Then soak it under a pressure of 0.2MPa for 90min. Then increase the pressure from 0.2MPa to 90MPa within 1s for extraction. After extraction, filter it with a ceramic membrane and collect the filtrate. Add 200μL of HindIII restriction endonuclease with a concentration of 10U / μL to the filtrate and enzymatically hydrolyze it at 22℃ for 150min. After enzymatic hydrolysis, inactivate the enzyme to obtain extract A.

[0067] (2) The remaining raw materials, except for ergothioneine, were pulverized to 200 mesh and mixed with 20 times their weight of water (at a temperature of 20±5℃). The mixture was then soaked for 90 minutes under a pressure of 0.2 MPa. The pressure was then increased from 0.2 MPa to 90 MPa within 1 second for extraction. After extraction, the mixture was filtered through a ceramic membrane and the filtrate was collected to obtain extract B.

[0068] (3) Mix extract A, extract B and ergothioneine, and then concentrate by reverse osmosis membrane to obtain herbal composition.

[0069] The preparation methods of the herbal compositions provided in Examples 2-7 and Comparative Examples 1-5 are consistent with those in Example 1.

[0070] Example 8

[0071] This invention provides a herbal composition, the only difference between the herbal composition and Example 1 is that in the preparation method, steps (1) and (2) involve increasing the pressure from 0.2 MPa to 80 MPa within 1 second for extraction.

[0072] Example 9

[0073] This invention provides a herbal composition, the only difference between the herbal composition and Example 1 is that in the preparation method, steps (1) and (2) involve increasing the pressure from 0.2 MPa to 70 MPa within 1 second for extraction.

[0074] Example 10

[0075] This invention provides a herbal composition, the only difference between the herbal composition and Example 1 is that in the preparation method, steps (1) and (2) involve increasing the pressure from 0.2 MPa to 50 MPa within 1 second for extraction.

[0076] Example 11

[0077] This invention provides a herbal composition, the only difference between the herbal composition and Example 1 is that in the preparation method, steps (1) and (2) involve increasing the pressure from 0.2 MPa to 90 MPa within 5 seconds for extraction.

[0078] Comparative Example 6

[0079] The present invention provides a comparative example of an herbal composition, the only difference between the herbal composition and Example 1 being the use of bergamot fruit instead of citron.

[0080] Comparative Example 7

[0081] The present invention provides a comparative example of a herbal composition, the only difference between the herbal composition and Example 1 being that rhubarb is used instead of Astragalus membranaceus root.

[0082] Comparative Example 8

[0083] The present invention provides a comparative example of a herbal composition, the only difference between the herbal composition and Example 1 being that roses are used instead of cherry blossoms.

[0084] Comparative Example 9

[0085] The present invention provides a comparative example of a herbal composition, the only difference between the herbal composition and Example 1 being that wolfberry is used instead of cornus officinalis.

[0086] Comparative Example 10

[0087] The present invention provides a comparative example of an herbal composition, the only difference between the herbal composition and Example 1 being the use of thiotaurine instead of ergothioneine.

[0088] Application Example 1

[0089] This invention provides a whitening and repairing essence mask, comprising the following components by weight: 20 parts of the herbal composition prepared in Example 1, 0.33 parts of sodium hyaluronate, 0.01 parts of disodium EDTA, 5 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 2 parts of glyceryl polyether-26, 2 parts of trehalose, 0.03 parts of β-glucan, 1 part of panthenol, 1 part of glyceryl glucoside, 0.1 parts of tranexamic acid, 0.03 parts of tremella polysaccharide, 0.2 parts of VC ethyl ether, and 67.8 parts of water;

[0090] The preparation method of the whitening and repairing essence mask includes the following steps:

[0091] (1) Add water, propylene glycol, disodium EDTA, sodium hyaluronate and β-glucan to a stirring emulsifying pot, heat to 80℃ (±3℃), homogenize at 3000 rpm / min for 8 min, stir at 25 rpm / min for 15 min, and then start cooling.

[0092] (2) When the temperature drops to 60℃ (±3℃), add p-hydroxyacetophenone, trehalose, panthenol and tremella polysaccharide, homogenize at 2500 rpm / min for 5 min, stir at 20 rpm / min for 10 min, and continue to cool down.

[0093] (3) When the temperature drops to 45℃ (±3℃), add glycerol polyether-26, glycerol glucoside, tranexamic acid, and VC ethyl ether, and continue stirring at the original speed while continuing to cool down;

[0094] (4) When the temperature drops to 30℃ (±2℃), add the herbal composition and stir at 20 rpm / min for 20 min until the system is uniform. Stop stirring, take a sample for testing, and after passing the test, discharge the material through a 500-mesh filter and let it stand for 24 h.

[0095] (5) After the material has been left to stand for 24 hours, it is filled into 25mL / tablets using an automatic filling machine, and then packaged.

[0096] Application Example 2

[0097] This invention provides a whitening and repairing essence, comprising the following components by weight: 20 parts of the herbal composition prepared in Example 1, 0.33 parts of sodium hyaluronate, 0.01 parts of disodium EDTA, 5 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 4 parts of glyceryl polyether-26, 0.5 parts of trehalose, 0.03 parts of β-glucan, 1 part of panthenol, 2 parts of glyceryl glucoside, 0.1 parts of tranexamic acid, 0.03 parts of tremella polysaccharide, 0.2 parts of VC ethyl ether, 3.5 parts of niacinamide, and 61.8 parts of water;

[0098] The preparation method of the whitening and repairing essence includes the following steps:

[0099] (1) Add water, propylene glycol, disodium EDTA, sodium hyaluronate and β-glucan to a stirring emulsifying pot, heat to 80℃ (±3℃), homogenize at 3000 rpm / min for 8 min, stir at 25 rpm / min for 15 min, and then start cooling.

[0100] (2) When the temperature drops to 60℃ (±3℃), add p-hydroxyacetophenone, trehalose, panthenol and tremella polysaccharide, homogenize at 2500 rpm / min for 5 min, stir at 20 rpm / min for 10 min, and continue to cool down.

[0101] (3) When the temperature drops to 45℃ (±3℃), add glycerol polyether-26, glycerol glucoside, tranexamic acid, VC ethyl ether and nicotinamide, continue stirring at the original speed and continue to cool down;

[0102] (4) When the temperature drops to 30℃ (±2℃), add the herbal composition and stir at 20 rpm / min for 20 min until the system is uniform. Stop stirring, take a sample for testing, and after passing the test, discharge the material through a 500-mesh filter and let it stand for 24 h.

[0103] (5) After the material has been left to stand for 24 hours, it can be directly filled and then packaged.

[0104] Application Example 3

[0105] This invention provides a whitening and repairing essence lotion, comprising the following components by weight: 20 parts of the herbal composition prepared in Example 1, 0.33 parts of sodium hyaluronate, 0.1 parts of carbomer 940, 0.01 parts of disodium EDTA, 5 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 2 parts of glyceryl polyether-26, 0.5 parts of trehalose, 0.03 parts of β-glucan, 1 part of panthenol, 1 part of glyceryl glucoside, 0.1 parts of tranexamic acid, 0.03 parts of tremella polysaccharide, 0.2 parts of VC ethyl ether, 3.5 parts of niacinamide, 2 parts of cetearyl alcohol, 1 part of isononyl isononanoate, 2 parts of C12-15 benzoate, 0.7 parts of monoglyceride, 1 part of vitamin E, 0.6 parts of A165, 2 parts of GTCC, 0.03 parts of aminomethylpropanol, and 56.47 parts of water.

[0106] The preparation method of the above-mentioned whitening and repairing essence lotion includes the following steps:

[0107] (1) Add water, propylene glycol, disodium EDTA, sodium hyaluronate, β-glucan, and carbomer 940 to a stirring emulsifying pot, heat to 80℃ (±3℃), homogenize at 3000 rpm / min for 8 min, stir at 25 rpm / min for 15 min, and maintain the temperature at 80℃.

[0108] (2) Add cetearyl alcohol, isononyl isononanoate, C12-15 alcohol benzoate, monoglyceride, vitamin E, and A165 to the oil phase pot, heat to 80℃ (±3℃), stir until the system is completely dissolved, and keep warm and stir for 20 minutes.

[0109] (3) Transfer the oil phase into the stirring emulsification pot, and homogenize it at 3000 rpm / min for 10 min, stir for 10 min, and start cooling.

[0110] (4) When the temperature drops to 60℃ (±3℃), add p-hydroxyacetophenone, trehalose, panthenol and tremella polysaccharide, homogenize at 2500 rpm / min for 5 min, stir at 20 rpm / min for 10 min, and continue to cool down.

[0111] (5) When the temperature drops to 45℃ (±3℃), add glycerol polyether-26, glycerol glucoside, tranexamic acid, VC ethyl ether and nicotinamide, continue stirring at the original speed and continue to cool down;

[0112] (6) When the temperature drops to 30℃ (±2℃), add the herbal composition and stir at 20 rpm / min for 20 min until the system is uniform. Stop stirring, take a sample for testing, and after passing the test, discharge the material through a 200-mesh filter and let it stand for 24 h.

[0113] (7) After the material has been left to stand for 24 hours, it can be directly filled and then packaged.

[0114] Application Example 4

[0115] This invention provides a whitening and repairing essence cream, comprising the following components by weight: 20 parts of the herbal composition prepared in Example 1, 0.33 parts of sodium hyaluronate, 0.25 parts of carbomer 940, 0.01 parts of disodium EDTA, 5 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 2 parts of glyceryl polyether-26, 0.5 parts of trehalose, 0.05 parts of β-glucan, 2 parts of panthenol, 1 part of glyceryl glucoside, 0.1 parts of tranexamic acid, 0.03 parts of tremella polysaccharide, 0.2 parts of VC ethyl ether, 3.5 parts of niacinamide, 3 parts of cetearyl alcohol, 3 parts of isononyl isononanoate, 2 parts of C12-15 benzoate, 1 part of monoglyceride, 1.5 parts of vitamin E, 1.2 parts of A165, 3 parts of GTCC, 0.08 parts of aminomethylpropanol, and 49.75 parts of water.

[0116] The preparation method of the whitening and repairing essence cream includes the following steps:

[0117] (1) Add water, propylene glycol, disodium EDTA, sodium hyaluronate, β-glucan, and carbomer 940 to a stirring emulsifying pot, heat to 80℃ (±3℃), homogenize at 3000 rpm / min for 8 min, stir at 25 rpm / min for 15 min, and maintain the temperature at 80℃.

[0118] (2) Add cetearyl alcohol, isononyl isononanoate, C12-15 alcohol benzoate, monoglyceride, vitamin E, and A165 to the oil phase pot, heat to 80℃ (±3℃), stir until the system is completely dissolved, and keep warm and stir for 20 minutes.

[0119] (3) Transfer the oil phase into the stirring emulsification pot, and homogenize it at 3000 rpm / min for 10 min, stir for 10 min, and start cooling.

[0120] (4) When the temperature drops to 60℃ (±3℃), add p-hydroxyacetophenone, trehalose, panthenol and tremella polysaccharide, homogenize at 2500 rpm / min for 5 min, stir at 20 rpm / min for 10 min, and continue to cool down.

[0121] (5) When the temperature drops to 45℃ (±3℃), add glycerol polyether ~26, glycerol glucoside, tranexamic acid, VC ethyl ether and nicotinamide, and continue stirring at the original speed while continuing to cool down;

[0122] (6) When the temperature drops to 30℃ (±2℃), add the herbal composition and stir at 20 rpm / min for 20 min until the system is uniform. Stop stirring, take a sample for testing, and after passing the test, discharge the material through a 200-mesh filter and let it stand for 24 h.

[0123] (7) After the material has been left to stand for 24 hours, it can be directly filled and then packaged.

[0124] Application Example 5

[0125] In an application embodiment of the present invention, a whitening and repairing essence eye cream comprises the following components by weight: 20 parts of the herbal composition prepared in Example 1, 0.33 parts of sodium hyaluronate, 0.25 parts of carbomer U20, 0.01 parts of disodium EDTA, 5 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 2 parts of glyceryl polyether-26, 0.5 parts of trehalose, 0.05 parts of β-glucan, 2 parts of panthenol, 1 part of glyceryl glucoside, 0.1 parts of tranexamic acid, 0.03 parts of tremella polysaccharide, 0.2 parts of VC ethyl ether, 3.5 parts of niacinamide, 0.2 parts of allantoin, 3 parts of cetearyl alcohol, 3 parts of isononyl isononanoate, 2 parts of plant squalane, 1 part of monoglyceride, 1.5 parts of vitamin E, 1.2 parts of A165, 3 parts of GTCC, 3 parts of grape seed oil, 0.2 parts of arginine, 1 part of palmitoyl pentapeptide-5, and 1 part of acetyl hexapeptide-8. 1 part, water 44.43 parts;

[0126] The preparation method of the whitening and repairing essence eye cream includes the following steps:

[0127] (1) Add water, propylene glycol, disodium EDTA, sodium hyaluronate, β-glucan, and carbomer U20 to a stirring emulsifying pot, heat to 80℃ (±3℃), homogenize at 3000 rpm / min for 8 min, stir at 25 rpm / min for 15 min, and maintain the temperature at 80℃.

[0128] (2) Add cetearyl alcohol, isononyl isononanoate, C12-15 alcohol benzoate, monoglyceride, vitamin E, A165, plant squalane, and GTCC to the oil phase pot, heat to 80℃ (±3℃), stir until the system is completely dissolved, and keep warm and stir for 20 minutes.

[0129] (3) Transfer the oil phase into the stirring emulsification pot, and homogenize it at 3000 rpm / min for 10 min, stir for 10 min, and start cooling.

[0130] (4) When the temperature drops to 60℃ (±3℃), add p-hydroxyacetophenone, trehalose, panthenol and tremella polysaccharide, homogenize at 2500 rpm / min for 5 min, stir at 20 rpm / min for 10 min, and continue to cool down.

[0131] (5) When the temperature drops to 45℃ (±3℃), add glycerol polyether ~26, glycerol glucoside, arginine, VC ethyl ether, and nicotinamide, and continue stirring at the original speed while continuing to cool down;

[0132] (6) When the temperature drops to 30℃ (±2℃), add the herbal composition, palmitoyl pentapeptide-5, and acetyl hexapeptide-8, and stir at 20 rpm / min for 20 min until the system is uniform. Stop stirring, take a sample for testing, and after passing the test, discharge the material through a 200-mesh filter and let it stand for 24 h.

[0133] (7) After the material has been left to stand for 24 hours, it can be directly filled and then packaged.

[0134] Example of effect 1

[0135] The efficacy of the herbal compositions prepared in Examples 1-11 and Comparative Examples 1-10 is verified by the following examples:

[0136] 1. Safety Verification: The safety of the herbal compositions prepared in Examples 1-11 and Comparative Examples 1-10 was investigated using the MTT assay to detect cell viability and growth. Specifically, cells (1*10⁻⁶) were used. 5 Cells / wells were incubated with the herbal composition at concentrations of 0.5-50% for 5 days. Even at the highest concentration of the herbal composition (50%), cell viability was greater than 90%, therefore the herbal composition is safe.

[0137] 2. Antioxidant effect: DPPH free radical scavenging experiment was conducted according to T / SHRH 006-2018, and the DPPH free radical scavenging rate was calculated and recorded; vitamin E, a positive control, was added in each experiment to confirm the effectiveness of the experimental system;

[0138] 3. Moisturizing effect: The test was conducted according to QBT 4256-2011, with 220 volunteers participating. Each group consisted of 10 volunteers. The test site was changed from the inner forearm in QBT 4256-2011 to the left and right sides of the face, while other aspects remained unchanged. Skin moisture content was recorded before application and 2 hours after application. The rate of change was calculated and recorded as follows: Moisture content change rate = (Skin moisture content 2 hours after application - Skin moisture content before application) / Moisture content before application * 100%. The average moisture content change rate for each group of volunteers was recorded. A positive control experiment was added, with glycerin added in each experiment to confirm the effectiveness of the test system.

[0139] 4. Whitening effect: The activity of tyrosinase was tested according to T / SHRH 015-2018, and the tyrosinase inhibition rate was calculated and recorded; kojic acid, a positive control, was added in each experiment to confirm the effectiveness of the experimental system.

[0140] The test results are shown in Table 2.

[0141] Table 2

[0142]

[0143]

[0144] As can be seen from Table 2, the herbal extract obtained by using the technical solution of the present invention has excellent moisturizing, antioxidant and whitening effects; specifically, the obtained product has a DPPH free radical scavenging rate of more than 35.36%, an increase in moisture content of more than 70.78% after 2 hours of use, and a tyrosinase inhibition rate of more than 32.23%.

[0145] As can be seen from Examples 1-4, the weight proportions of raw materials affect the overall performance of the product. When the weight proportions of the selected raw materials are within the range given in this invention, the overall effect of the product is better, specifically, the DPPH free radical scavenging rate is above 58.39%, the increase in moisture content after 2 hours of use is above 88.52%, and the tyrosinase inhibition rate is above 52.34%. As can be seen from Examples 1 and Examples 5-6, the weight ratio of citron, astragalus root, cherry blossom, and cornus officinalis also affects the overall performance of the product. When the weight ratio of citron, astragalus root, cherry blossom, and cornus is further selected as citron:astragalus root:cherry blossom:cornus = 1:(0.8-1.2):(1.2-2):(1.2-2), the overall effect of the product is better. Among them, the DPPH free radical scavenging rate is above 60.96%, the increase in moisture content after 2 hours of use is above 90.10%, and the tyrosinase inhibition rate is above 52.98%. As can be seen from Examples 1 and Examples 8-11, the preparation method of the herbal composition also affects the performance of the product.

[0146] As can be seen from Examples 1 and Comparative Examples 1-5, the raw material components in the composition are indispensable. When any one of the components is missing, even if the concentration of the analyte added during verification is the same, the effect obtained will be significantly reduced. As can be seen from Examples 1 and Comparative Examples 6-10, when other similar components are used to replace the raw materials in the present invention, the corresponding effect cannot be obtained.

[0147] Example 2

[0148] The present invention provides an example to verify the practical application effect of the prepared herbal composition. Specifically, the herbal composition prepared in Example 1 was mixed with sodium hyaluronate, propylene glycol and p-hydroxyacetophenone to obtain a corresponding whitening and repairing composition sample. The components (parts by mass) of the verification example are shown in Table 3.

[0149] Table 3

[0150] Verification Example 1 Verification Example 2 Verification Example 3 Verification Example 4 Blank example Herbal Composition 10 20 30 50 0 Sodium hyaluronate 0.1 0.1 0.1 0.1 0.1 Propylene glycol 30 30 30 30 30 p-Hydroxyacetophenone 0.3 0.3 0.3 0.3 0.3

[0151] The preparation method of the whitening and repairing composition sample is as follows: the herbal composition, sodium hyaluronate, propylene glycol and p-hydroxyacetophenone are mixed evenly to obtain the whitening and repairing composition sample;

[0152] The prepared whitening and repairing composition sample was subjected to a whitening and repairing sensitive skin test, specifically including the following:

[0153] (1) The testing period is from January to March each year. 150 volunteers with sensitive skin aged between 25 and 55 years old are randomly selected and divided into five groups of 30 people each. Among them, the people in the first, second, third and fourth groups use the whitening and repairing composition samples prepared in verification examples 1-4 respectively, and the people in the fifth group use the blank sample. The testing period is 5 weeks.

[0154] (2) The method of use is as follows: Do not use any cosmetics for one week before the test. When the test begins, use the whitening and repairing composition sample of the present invention on the face and neck every morning and evening. Gently massage until absorbed. Do not use other cosmetics during the test. Test the sample after the second week.

[0155] (3) The evaluation levels for the face and neck are as follows: Level I, the skin is fair, moisturized and more translucent; the skin sensitivity has been completely repaired and cured; Level II, the skin is brighter and more translucent than before; the skin sensitivity has been repaired to some extent; Level III, the skin is brighter and more translucent than before; the skin sensitivity has not been eliminated to some extent, and sensitivity still occurs from time to time.

[0156] (4) The test results are shown in Table 4 below;

[0157] Table 4

[0158]

[0159]

[0160] Meanwhile, six volunteers were randomly selected from groups one, two, three, and four (ensuring one volunteer from each group) to provide before-and-after comparison photos of their use of the whitening and repairing composition sample, such as... Figure 1-6 As shown in the attached images (left side shows before use, right side shows after use), comparison images of two volunteers in the blank group before and after use are also provided. Figure 7-8 As shown;

[0161] From Table 4 and Figure 1-6As can be seen, the whitening composition containing the herbal composition of the present invention has been verified in terms of skin whitening, and this whitening composition has a whitening and brightening effect on the skin; in terms of repairing sensitive skin, its repair effect has reached over 90%, especially for redness, swelling, itching, and sunburn after microneedling and laser treatment, and it also has a good effect on repairing fine lines; at the same time, from Figure 1-6 It can be seen from the comparison before and after use by volunteers, and with... Figure 7-8 In the before-and-after comparison of the control group, it can be seen with the naked eye that the sensitive skin areas on the volunteers' faces have been relieved, and the spots on their faces have been significantly faded, with a certain whitening and brightening effect.

[0162] Example 3

[0163] The effect of the mask prepared in Example 1 of this invention is verified by the following examples:

[0164] (1) Ten volunteers with sensitive skin aged between 25 and 55 were randomly selected;

[0165] (2) You must wash your face before each test. After washing your face, start timing. Use 0min, 10min, 20min and 30min as test points. Use a skin moisture meter to test the skin moisture value, record the test value and calculate the average value. When testing the value, divide it into left face and right face. The first test is conducted in this way. The value obtained is the value of using a face mask once.

[0166] (3) After using the mask for 2 weeks, test it according to the method in point 1 and record the test data. The data is the value after using the mask for 2 weeks.

[0167] (4) While still using the mask once a day, after 4 weeks of use, test again using the method in point 1. The value is the value after 4 weeks of using the mask.

[0168] The results are shown in Table 5.

[0169] Table 5

[0170]

[0171]

[0172] The data in Table 5 were used to create a bar chart, and the results are as follows: Figure 9-10 As shown, where Figure 9 A graph showing the changes in the skin's moisture retention on the right side of the face. Figure 10The graph shows the changes in the skin's moisture retention performance on the left side of the face. As can be seen from the graph, using a mask containing the herbal composition of this invention can effectively help the skin retain moisture, and the longer the continuous use, the more significant the improvement in the skin's moisture retention ability.

[0173] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A herbal composition, characterized in that, The herbal composition comprises the following raw materials in parts by weight: 5-30 parts of *Hypericum gloeocarpus*, 5-30 parts of *Citrus medica*, 5-30 parts of *Portulaca oleracea*, 5-30 parts of *Chamomile*, 5-30 parts of *Astragalus membranaceus* root, 10-50 parts of cherry blossom, 10-50 parts of *Polygonum cuspidatum*, 10-50 parts of *Cornus officinalis*, 10-50 parts of licorice, 5-20 parts of ergothioneine, 5-30 parts of *Sophora japonica* fruit, 5-30 parts of Sichuan pepper, 5-20 parts of forsythia, and 10-50 parts of *Chrysanthemum indicum*. The method for preparing the herbal composition includes the following steps: (1) After crushing the wound-healing velvet flower, mix it with water, then soak it under pressure. After soaking for a period of time, extract it under pressure. After extraction, filter it and collect the filtrate for enzymatic hydrolysis. After enzymatic hydrolysis, extract A is obtained. (2) The remaining raw materials, except for ergothioneine, are crushed and mixed with water. Then, they are soaked under pressure. After soaking for a period of time, they are extracted under pressure. After extraction, they are filtered and the filtrate is collected to obtain extract B. (3) Mix and concentrate extract A, extract B and ergothioneine to obtain herbal composition; In the pressurized extraction process, the pressure is increased from 0.2 MPa to over 70 MPa within 0.5-1 s. The enzyme used in the enzymatic hydrolysis is Hind III, the hydrolysis time is 140-160 min, and the hydrolysis temperature is 20-25℃. The enzymatic hydrolysis process also includes an enzyme inactivation treatment, specifically: incubation at 80-90℃ for 15-25 minutes.

2. The herbal composition according to claim 1, characterized in that, The weight ratio of citron, Astragalus membranaceus root, cherry blossom, and Cornus officinalis in the raw materials is citron: Astragalus membranaceus root: cherry blossom: Cornus officinalis = 1: (0.8-1.2): (1.2-2): (1.2-2).

3. The method for preparing the herbal composition according to any one of claims 1-2, characterized in that, The preparation method includes the following steps: (1) After crushing the wound-healing velvet flower, mix it with water, then soak it under pressure. After soaking for a period of time, extract it under pressure. After extraction, filter it and collect the filtrate for enzymatic hydrolysis. After enzymatic hydrolysis, extract A is obtained. (2) The remaining raw materials, except for ergothioneine, are crushed and mixed with water. Then, they are soaked under pressure. After soaking for a period of time, the pressure is increased for extraction. After extraction, the mixture is filtered and the filtrate is collected to obtain extract B. (3) Mix and concentrate extract A, extract B and ergothioneine to obtain herbal composition; In the pressurized extraction process, the pressure is increased from 0.2 MPa to over 70 MPa within 0.5-1 s. The enzyme used in the enzymatic hydrolysis is Hind III, the hydrolysis time is 140-160 min, and the hydrolysis temperature is 20-25℃. The enzymatic hydrolysis process also includes an enzyme inactivation treatment, specifically: incubation at 80-90℃ for 15-25 minutes.

4. The preparation method according to claim 3, characterized in that, At least one of the following (a)-(b): (a) The soaking time is 60-120 min, and the solid-liquid ratio is 10-30; (b) The temperature of the water is 15-30°C.

5. The use of the herbal composition according to any one of claims 1-2 in the preparation of skin care products.

6. A skincare product, characterized in that, The skincare product includes the herbal composition as described in any one of claims 1-2.

7. The skincare product according to claim 6, characterized in that, Based on the skincare product, the herbal composition comprises 0.5-30% by mass.

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