Anti-aging composition, single-use ampoule and its preparation method

Abstract

This invention belongs to the field of cosmetics, specifically relating to an anti-aging composition, a single-use ampoule, and its preparation method. The anti-aging composition comprises the following components: recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate. This invention, through the synergistic effect of recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate, supplements and promotes collagen function, thereby better resisting wrinkle formation and providing protection against blue light and ultraviolet rays.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics, specifically relating to an anti-aging composition, a single-use ampoule, and a method for preparing the same. Background Technology

[0002] Skincare products, applied to the skin, can moisturize and hydrate. Especially in the dry autumn and winter seasons, skincare products can increase the skin's oil and moisture content, preventing dryness and cracking, and leaving the skin more radiant. However, the ingredients and additives in skincare products vary greatly, and their functions differ depending on the added ingredients. Aging is a gradual and irreversible decline in physiological structure and function that occurs from the organism's maturity stage with increasing age. Skin aging is generally divided into endogenous aging and exogenous aging: Endogenous aging refers to natural aging that occurs with increasing age. Natural aging is mainly due to the decreased ability of collagen and elastin synthesis in the skin as we age and with physiological aging, leading to the degeneration of elastic tissue and resulting in loose skin. Exogenous aging mainly refers to photoaging caused by sun exposure. Photoaging is mainly caused by ultraviolet rays penetrating the skin and reaching below the dermis, damaging the fine structures inside the skin, such as collagen and elastic fibers, thus leading to wrinkles and age spots.

[0003] Collagen forms the framework of the skin, which contains approximately 40% of the body's total collagen. The aging process of the skin is also a process of collagen loss. The skin's collagen content reaches its peak between the ages of 20 and 30, after which collagen degrades, decreasing at a rate of 1% to 1.5% per year. Type III collagen levels begin to decline from birth.

[0004] As we age, the synthetic capacity of fibroblasts declines. If the skin lacks collagen, collagen fibers will become rigid, reducing intercellular mucopolysaccharides. As a result, the skin loses its softness, elasticity, and luster, leading to aging. At the same time, dermal fiber breakage, fat atrophy, and reduced secretion from sweat and sebaceous glands cause wrinkles and other signs of aging. Adding collagen as an active ingredient to skincare products can effectively improve skin aging. Therefore, developing a skincare product containing collagen is essential for improving skin aging. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide an anti-aging composition, a single-use serum, and a method for preparing the same.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] An anti-aging composition comprising the following components: recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate.

[0008] Preferably, the product comprises the following components in parts by weight: 1-3 parts of recombinant type III humanized collagen, 1-3 parts of hydrolyzed lupin protein, and 1-2 parts of lactic acid bacteria fermentation lysate.

[0009] 2-3 parts of recombinant type III humanized collagen, 2-3 parts of hydrolyzed lupin protein, and 1-2 parts of lactic acid bacteria fermentation lysate.

[0010] The present invention also includes a single-use serum comprising the aforementioned anti-aging composition.

[0011] Preferably, the composition comprises the following components in weight percentages: 1-8% glycerol, 3-5% butylene glycol, 1-3% squalane, 0.2-1% ammonium acryloyldimethyl taurate / VP copolymer, 3-8% nicotinamide, 0.1-0.5% carbomer, 3-8% of the anti-aging composition, citric acid and a buffer solution of citric acid, and water. Its pH value is 6.0-7.0.

[0012] Preferably, the composition comprises the following components in weight percentages: 5% glycerol, 4% butylene glycol, 2% squalane, 0.6% ammonium acryloyldimethyl taurate / VP copolymer, 4% nicotinamide, 0.2% carbomer, 5% of the anti-aging composition, citric acid and a buffer solution of citric acid, and water.

[0013] This invention also includes a method for preparing the aforementioned single-use serum, comprising the following steps:

[0014] (1) Mix glycerol, nicotinamide, squalane, butanediol and water and heat to 85°C to obtain solution A;

[0015] (2) Add the soaked ammonium acryloyl dimethyl taurate / VP copolymer to A and homogenize for 1 minute to obtain solution B;

[0016] (3) Add the prepared citric acid and citric acid buffer solution to solution B to adjust the pH value to 6.0-7.0 to obtain solution C, and stir and cool.

[0017] (4) After the solution C is cooled to 55°C, hydrolyzed lupin protein, recombinant type III humanized collagen and lactic acid bacteria fermentation lysate from the multi-effect composition are added to the solution C to obtain a single-use essence.

[0018] Compared with the prior art, the beneficial effects of the present invention are:

[0019] This invention provides a single-use serum containing recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate. It can replenish type III collagen and promote the regeneration of type III collagen, while the lactic acid bacteria fermentation lysate can protect against blue light.

[0020] Recombinant type III humanized collagen plays a crucial role in skin damage repair and reconstruction. It improves the transmembrane transport of water molecules across cell membranes; this collagen readily binds to water molecules, exhibiting excellent hydrophilicity and water-binding properties, and promotes hyaluronic acid synthesis. It significantly thickens the stratum corneum, enhances skin barrier function, and promotes the expression of phytohexin and serine. It repairs the skin barrier, alleviates inflammation, and replenishes lost collagen.

[0021] Hydrolyzed lupin protein can promote the production of type III collagen, a major component of the skin's extracellular matrix, which can form a three-dimensional network, making the skin firmer and more resilient. Its effects include enhancing the connection between the dermis and epidermis, strengthening dermal tissue, and tightening the skin.

[0022] Lactic acid bacteria fermentation lysates can correct the skin's natural biological rhythms, resynchronize the biological rhythm disorders caused by excessive blue light exposure, significantly increase the positive regulation of melatonin, and have an overall healthy and aging effect on the skin.

[0023] This serum is preservative-free, making it safer, gentler, and non-irritating.

[0024] In summary, through the synergistic effect of recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysates, collagen firstly penetrates deep into the skin to reduce inflammation, regulate sebum secretion, and replenish lost collagen, thus enhancing the skin's barrier function and improving the sebum film. Secondly, hydrolyzed lupin protein further promotes the production of type III collagen, replenishing and promoting collagen to plump the skin, thereby playing an anti-aging role. Finally, the lactic acid bacteria fermentation lysates provide blue light protection and increase positive regulation of melatonin, contributing to overall healthy aging of the skin. The interaction of endogenous and exogenous factors synergistically replenishes and promotes collagen production, better resisting wrinkle formation and providing protection against blue light and ultraviolet rays. Attached Figure Description

[0025] Figure 1 This is a graph showing the changes in matrix metalloproteinases (MMPs) in the examples and control examples;

[0026] Figure 2 The figures show cell migration results for the examples and control examples.

[0027] Figure 3 The graph shows the results of the effects of the examples and control examples on SOD activity. Detailed Implementation

[0028] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and preferred embodiments; unless otherwise specified, all products used in this application are commercially available products.

[0029] Example 1

[0030] A single-use serum comprises the following components: 5g glycerin, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 5g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the balance being deionized water to 100g; the anti-aging composition comprises 2g hydrolyzed lupin protein, 2g recombinant type III humanized collagen (hereinafter referred to as collagen, Shanxi Jinbo Biotechnology), and 1g lactic acid bacteria fermentation lysate.

[0031] The preparation method of single-use ampoule serum includes the following steps:

[0032] Glycerin, nicotinamide, squalane, butanediol, and water are mixed and heated to 85°C to obtain solution A.

[0033] (2) Add the soaked ammonium acryloyl dimethyl taurate / VP copolymer to A and homogenize for 1 minute to obtain solution B;

[0034] (3) Add the prepared citric acid and citric acid buffer solution to solution B to obtain solution C, stir and cool to adjust the pH value to 6.0-7.0;

[0035] (4) After the solution C is cooled to 55°C, hydrolyzed lupin protein, recombinant type III humanized collagen, and lactic acid bacteria fermentation lysate are added to the solution C to obtain a single-use essence.

[0036] Example 2

[0037] The difference between Example 2 and Example 1 is that the preparation components are different. Specifically, Example 2 includes: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 8g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the balance deionized water to 100g. The anti-aging composition includes 3g hydrolyzed lupin protein, 3g recombinant type III humanized collagen, and 2g lactic acid bacteria fermentation lysate.

[0038] Example 3

[0039] The difference between Example 3 and Example 1 is that the preparation components are different. Specifically, Example 3 includes: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 3g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the balance deionized water to 100g. The anti-aging composition includes 1g hydrolyzed lupin protein, 1g recombinant type III humanized collagen, and 1g lactic acid bacteria fermentation lysate.

[0040] Control group 1

[0041] The difference between Control Group 1 and Example 1 lies in the different preparation components, specifically including: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 3g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the remainder deionized water to 100g. The anti-aging composition includes 0g hydrolyzed lupin protein, 2g recombinant type III humanized collagen, and 1g lactic acid bacteria fermentation lysate.

[0042] Control group 2

[0043] The difference between Control Group 2 and Example 1 lies in the different preparation components, specifically including: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 4g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the remainder deionized water to 100g; the anti-aging composition includes 2g hydrolyzed lupin protein, 0g recombinant type III humanized collagen, and 2g lactic acid bacteria fermentation lysate.

[0044] Control group 3

[0045] The difference between Control Group 3 and Example 1 lies in the different preparation components, specifically including: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 6g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the remainder deionized water to 100g. The anti-aging composition includes 0g hydrolyzed lupin egg, 4g recombinant type III humanized collagen, and 2g lactic acid bacteria fermentation lysate.

[0046] Control group 4

[0047] The difference between Control Group 4 and Example 1 lies in the different preparation components, specifically including: 5g glycerol, 4g butylene glycol, 2g squalane, 0.6g ammonium acryloyldimethyl taurate / VP copolymer, 4g nicotinamide, 0.2g carbomer, 7g anti-aging composition, appropriate amounts of citric acid and sodium citrate buffer, and the remainder deionized water to 100g. The anti-aging composition includes 0g hydrolyzed lupin egg, 5g recombinant type III humanized collagen, and 2g lactic acid bacteria fermentation lysate.

[0048] Performance testing (the testing methods used in this application are all conventional testing methods, and the specific testing methods are not described in detail):

[0049] 1. Anti-aging effects - test to reduce collagen degradation:

[0050] When skin is exposed to ultraviolet radiation, the level of matrix metalloproteinases (MMPs) increases, leading to increased collagen degradation and significant collagen loss. Figure 1 This is a graph showing the changes in RNA expression of matrix metalloproteinases (MMPs) in the examples and control examples, observed over a period of 2 weeks. The results indicate that the combination of collagen with hydrolyzed lupin protein can reduce MMP content, thereby reducing collagen degradation.

[0051] 2. Fibroblast migration rate test:

[0052] Cell migration ability reflects the strength of cell polarity and activity; therefore, products can achieve anti-aging effects by promoting cell activity and polarity. Figure 2 Cell migration results for examples and comparative examples; by Figure 2 It can be seen that all 7 experimental groups have the effect of promoting cell migration, with improvement rates of 120%, 122%, 60%, 20%, 30%, 35%, and 40%, respectively. Examples 1 and 2 are significantly higher than the other control groups.

[0053] 3. Ultraviolet light aging test:

[0054] In a rat skin UV photoaging model, UV irradiation produces a large amount of ROS (reactive oxygen species), reduces the activity of SOD (superoxide dismutase), and leads to DNA damage in skin tissue. This composition can reduce photoaging and upregulate the activity of SOD. Figure 3 The graph shows the effect of SOD activity on the results; from Figure 3 It can be seen that the SOD content increased significantly in Examples 1 and 2, while Comparative Examples 1, 2, 3, and 4 had little effect on the SOD content. This indicates that the simultaneous use of the three compositions of collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate significantly improved SOD activity, while the combination of two or the use of each composition alone had no effect on the improvement of SOD activity.

[0055] In summary, the optimal ratio of collagen: hydrolyzed lupin protein: lactic acid bacteria fermentation lysate is 2-3:2-3:1-2, with the 2:2:1 ratio being the best due to its relatively small addition amount. Therefore, this is the optimal embodiment.

[0056] The technical solution of this application utilizes the synergistic effect of recombinant type III humanized collagen, hydrolyzed lupin protein, and lactic acid bacteria fermentation lysate. Firstly, the collagen penetrates deep into the skin to reduce inflammation, regulate sebum secretion, and replenish lost collagen, thus enhancing the skin's barrier function and improving the sebum film. Secondly, hydrolyzed lupin protein further promotes the production of type III collagen, replenishing and promoting collagen to plump the skin, thereby achieving an anti-aging effect. Finally, the lactic acid bacteria fermentation lysate provides blue light protection and increases positive regulation of melatonin, contributing to overall healthy aging of the skin. The interaction of endogenous and exogenous factors synergistically replenishes and promotes collagen production, better resisting wrinkle formation and providing protection against blue light and ultraviolet rays.

[0057] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-aging composition, characterized in that, It includes the following components by weight: 2-3 parts recombinant type III humanized collagen, 2-3 parts hydrolyzed lupin protein, and 1-2 parts lactic acid bacteria fermentation lysate.

2. A single-use essence, characterized in that, Includes the anti-aging composition according to claim 1.

3. A single-use serum as described in claim 2, characterized in that, It comprises the following components by weight percentage: 1-8% glycerol, 3-5% butylene glycol, 1-3% squalane, 0.2-1% ammonium acryloyldimethyl taurate / VP copolymer, 3-8% nicotinamide, 0.1-0.5% carbomer, 3-8% of the anti-aging composition, citric acid and a buffer solution of citric acid, and water.

4. The single-use serum according to claim 3, characterized in that, It comprises the following components by weight percentage: 5% glycerol, 4% butylene glycol, 2% squalane, 0.6% ammonium acryloyldimethyl taurate / VP copolymer, 4% nicotinamide, 0.2% carbomer, 5% of the anti-aging composition, citric acid and a buffer solution of citric acid, and water.

5. A method for preparing a single-use ampoule according to any one of claims 3-4, characterized in that, Includes the following steps: (1) Mix glycerol, nicotinamide, squalane, butanediol, and water, and heat to 85°C to obtain solution A; (2) Add the soaked ammonium acryloyldimethyl taurate / VP copolymer to A and homogenize for 1 minute to obtain solution B; (3) Add the prepared citric acid and citric acid buffer solution to solution B to obtain solution C, stir and cool to adjust the pH value to 6.0-7.0; (4) After the solution C is cooled to 55°C, hydrolyzed lupin protein, recombinant type III humanized collagen, and lactic acid bacteria fermentation lysate are added to the solution C to obtain a single-use essence.

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