Antibacterial and pro-angiogenic traditional Chinese medicine hydrogel, and preparation method and application thereof

Abstract

The application provides a novel hydrogel, which is called polyvinyl alcohol (PVA)-chitosan (CS) / sodium alginate (SA)-curcumin (Cur) (PCSA) transparent hydrogel. The hydrogel is prepared for the first time by using a Michael addition reaction of chitosan (CS) and Cur under alkali / urea conditions, exhibits strong mechanical properties, persistent antibacterial properties, promotes endothelial cell migration, tube formation and other characteristics, and provides a novel hydrogel dressing for treating a diabetic refractory wound.

Description

Technical Field

[0001] This invention relates to a traditional Chinese medicine hydrogel with antibacterial and angiogenesis-promoting properties, its preparation method, and its application, belonging to the field of biomaterials technology. Background Technology

[0002] Diabetic wounds often suffer from excessive inflammation and bacterial infection, hindering the skin's ability to regain its functional integrity over extended periods, thus burdening patients, society, and healthcare systems. In 2019, approximately 463 million adults worldwide had diabetes; this number is projected to reach 578 million by 2030. Therefore, there is a need to develop novel dressings that not only act as a physical barrier to prevent further wound damage and infection but also accelerate wound closure to promote healing and reduce scar formation. Curcumin, a traditional Chinese medicine, has shown great potential in promoting the healing of chronic diabetic wounds due to its immunomodulatory, hypoglycemic, and angiogenic properties. However, its limited water solubility, poor bioavailability, and poor chemical stability restrict its clinical application.

[0003] Therefore, it is of great significance to develop new cur loading and release pathways to improve its stability and bioavailability. Summary of the Invention

[0004] To overcome the problems existing in the prior art, this application develops a novel hydrogel (abbreviated as PCSA hydrogel), called polyvinyl alcohol (PVA)-chitosan (CS) / sodium alginate (SA)-Cur (PCSA) hydrogel. This hydrogel is prepared for the first time, utilizing the Michael addition reaction of chitosan (CS) and Cur under alkaline / urea conditions, exhibiting strong mechanical properties, long-lasting antibacterial properties, and the ability to promote endothelial cell migration and tubing formation, providing a novel hydrogel dressing for treating difficult-to-heal diabetic wounds.

[0005] A traditional Chinese medicine hydrogel with antibacterial and angiogenesis-promoting properties, comprising the following components in parts by weight:

[0006] PVA: 8-10 parts;

[0007] CS / SA-Cur pregel: 0.2–1.2 parts;

[0008] Water: 90-100 parts.

[0009] As a preferred embodiment, the shell CS / SA-Cur pregel comprises the following components in parts by weight:

[0010]

[0011]

[0012] As a preferred embodiment, the alkali / urea mixture comprises lithium hydroxide, potassium hydroxide, urea, and water, wherein the weight ratio of lithium hydroxide, potassium hydroxide, urea, and water is 4.5:7:8:80.5.

[0013] As a preferred embodiment, the method for preparing the CS / SA-Cur pregel is as follows:

[0014] CS and Cur were dispersed in an alkaline / urea aqueous solution, and SA was added after stirring. After mixing, the mixture was allowed to stand at -30 to -10°C for 12 hours, thawed at room temperature, and then allowed to stand at -90 to -70°C for 24 hours. This was recorded as one freeze-thaw cycle. After two freeze-thaw cycles, the mixture was centrifuged at room temperature, and the supernatant was collected to obtain the CS / SA-Cur pregel.

[0015] A method for preparing a traditional Chinese medicine hydrogel with antibacterial and angiogenesis-promoting properties, as described above, includes the following steps:

[0016] S1. Dissolve PVA in deionized water at 90℃ to obtain a uniform and transparent PVA solution;

[0017] S2. Add the CS / SA-Cur pregel to the PVA solution, stir and centrifuge to remove insoluble matter, freeze at -90 to -70°C for 24 hours, and thaw at room temperature for 2 to 4 hours. This is recorded as one cycle. Repeat the cycle three times to obtain the PCSA hydrogel composition.

[0018] Through the above technical solution, the molecular structures of CS and SA contain abundant active amino, carboxyl and hydroxyl groups, while the structure of Cur contains carbonyl and hydroxyl groups. Therefore, under alkaline conditions, CS, SA and Cur form covalent cross-links and non-covalent cross-links, causing CS / SA-Cur molecules to entangle with each other to form a hydrogel precursor. Then, it is combined with PVA to form a hydrogel with antibacterial, antioxidant and angiogenesis-promoting capabilities that promotes the repair of diabetic wounds.

[0019] The use of a traditional Chinese medicine hydrogel with antibacterial and angiogenesis properties, as described above, in wound healing materials.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) A curcumin-based hydrogel with transparent properties is obtained through the above technical solution.

[0022] (2) This invention uses PVA as the main material, SA and CS as auxiliary materials, and the natural antibacterial agent Cur as the active ingredient to prepare a hydrogel that promotes the repair of diabetic wounds with antibacterial, antioxidant, and angiogenesis-promoting abilities. The synergistic effect of CS and Cur gives the PCSA hydrogel excellent antibacterial ability, effectively keeping the wound in a sterile environment and avoiding prolonged infection of the wound due to the proliferation and adhesion of a large number of bacteria, thus creating favorable healing conditions for wound healing. Secondly, the SA molecular chain and the CS / Cur molecular chain are intertwined, so that Cur is released continuously and slowly, achieving long-term antibacterial effect.

[0023] (3) The PCSA hydrogel prepared by the present invention has strong mechanical properties. The appropriate mechanical properties can ensure the patient's daily activities and avoid the hydrogel from breaking due to the patient's activities, and the wound being exposed to air and thus infected by bacteria.

[0024] (4) The PCSA hydrogel prepared by this invention consists of all biocompatible natural materials. Moreover, the preparation process is simple and easy to operate. The active ingredients are released rapidly and slowly, which can produce a rapid response at the wound site, shorten the wound healing time, and improve the patient's happiness index. Attached Figure Description

[0025] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0026] Figure 1 The infrared spectrum of the pregel prepared in Comparative Example 1 and in Step S1 of Examples 1 and 7 of this invention;

[0027] Figure 2 The transparency properties of the PCSA hydrogels prepared in Examples 1, 5 and 7 of this invention;

[0028] Figure 3 The mechanical properties of the PCSA hydrogels prepared in Examples 1 and 7 of this invention are shown (a is tensile strength, b is compressive strength).

[0029] Figure 4 The antibacterial properties of the PCSA hydrogels prepared in Examples 1, 5 and 7 of this invention;

[0030] Figure 5 Cell compatibility of the PCSA hydrogels prepared in Examples 1, 5 and 7 of this invention;

[0031] Figure 6 The migration of human umbilical vein endothelial cells (HUVECs) in the PCSA hydrogels prepared in Examples 1, 5 and 7 of this invention;

[0032] Figure 7Angiogenic experiments of the PCSA hydrogels prepared in Examples 1, 5 and 7 of this invention;

[0033] Figure 8 This serves as a verification of the wound healing properties of the PCSA hydrogels prepared in Examples 1 and 7 of this invention. Detailed Implementation

[0034] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0035] Example 1

[0036] Preparation of PCSA1 hydrogel

[0037] Preparation of S1 and CS / SA-Cur pregels:

[0038] Weigh 1g of CS and 1g of SA and disperse them in 48g of an alkali / urea mixture (lithium hydroxide, potassium hydroxide, urea, and water in a weight ratio of 4.5:7:8:80.5). After stirring at room temperature for 30 minutes, add 0.1g of Cur to the mixture. Stir at room temperature for 30 minutes, then transfer to a -80℃ ultra-low temperature freezer for 24 hours. After removing it and allowing it to stand at room temperature for 4 hours, repeat the process three times. Centrifuge at 3000 rpm to remove the dissolved portion, collect the homogeneous solution, and obtain the CS / SA-Cur pregel, denoted as CSA-1.

[0039] Preparation of S2 and PCSA1 hydrogels

[0040] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 10g of CSA-1 prepared in step S1 was weighed and stirred at room temperature for 12 hours. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24 hours, followed by 4 hours at room temperature. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA1 hydrogel.

[0041] Example 2

[0042] Preparation of PCSA2 hydrogel

[0043] Preparation of S1 and CS / SA-Cur pregels:

[0044] Weigh 1g of CS and 1g of SA and disperse them in 48g of an alkali / urea mixture (lithium hydroxide, potassium hydroxide, urea, and water in a weight ratio of 4.5:7:8:80.5). After stirring at room temperature for 30 minutes, add 0.2g of Cur to the mixture. Stir at room temperature for 30 minutes, then transfer to a -80℃ ultra-low temperature freezer for 24 hours. After that, remove it and let it stand at room temperature for 4 hours. Repeat this process three times. Centrifuge at 3000 rpm to remove the dissolved portion, collect the homogeneous solution, and obtain the CS / SA-Cur pregel, denoted as CSA-2.

[0045] Preparation of S2 and PCSA2 hydrogels

[0046] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 4g of CSA-2 prepared in step S1 was weighed out and stirred at room temperature for 12 hours. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24 hours, followed by 4 hours at room temperature. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA2 hydrogel.

[0047] Example 3

[0048] Preparation of PCSA3 hydrogel

[0049] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 6g of CSA-2 prepared in step S1 of Example 2 was weighed out and stirred at room temperature for 12h. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24h. After that, it was taken out and placed at room temperature for 4h. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA3 hydrogel.

[0050] Example 4

[0051] Preparation of PCSA4 hydrogel

[0052] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 8g of CSA-2 prepared in step S1 of Example 2 was weighed and stirred at room temperature for 12h. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24h. After that, it was taken out and placed at room temperature for 4h. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA4 hydrogel.

[0053] Example 5

[0054] Preparation of PCSA5 hydrogel

[0055] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 10g of CSA-2 prepared in step S1 of Example 2 was weighed and stirred at room temperature for 12h. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24h. After that, it was taken out and placed at room temperature for 4h. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA5 hydrogel.

[0056] Example 6

[0057] Preparation of PCSA6 hydrogel

[0058] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 12g of CSA-2 prepared in step S1 of Example 2 was weighed and stirred at room temperature for 12h. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24h. After that, it was taken out and placed at room temperature for 4h. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA6 hydrogel.

[0059] Example 7

[0060] Preparation of PCSA7 hydrogel

[0061] Preparation of S1 and CS / SA-Cur pregels:

[0062] Weigh 1g of CS and 1g of SA and disperse them in 48g of an alkali / urea mixture (lithium hydroxide, potassium hydroxide, urea, and water in a weight ratio of 4.5:7:8:80.5). After stirring at room temperature for 30 minutes, add 0.3g of Cur to the mixture. Stir at room temperature for 30 minutes, then transfer to a -80℃ ultra-low temperature freezer for 24 hours. After removing it and allowing it to stand at room temperature for 4 hours, repeat the process three times. Centrifuge at 3000 rpm to remove the dissolved portion, collect the homogeneous solution, and obtain the CS / SA-Cur pregel, denoted as CSA-3.

[0063] Preparation of S2 and PCSA7 hydrogels

[0064] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, 10g of CSA-3 prepared in step S1 was weighed and stirred at room temperature for 12 hours. The insoluble matter was removed by centrifugation, and a homogeneous and transparent mixture was collected. This mixture was then transferred to an ultra-low temperature freezer at -80℃ and placed for 24 hours, followed by 4 hours at room temperature. This process was repeated three times. Finally, the mixture was placed in deionized water to remove the alkali / urea, yielding PCSA7 hydrogel.

[0065] Comparative Example 1

[0066] The only difference between Comparative Example 1 and Step S1 in Examples 1 and 7 is that SA is not added, only CS and Cur are used.

[0067] Comparative Example 2

[0068] The only difference between Comparative Example 1 and Example 1 is that Cur was not added in step S1 to prepare a pregel, denoted as CSA-0, and the prepared hydrogel was denoted as PCSA0.

[0069] Comparative Example 3

[0070] 10g of PVA was dispersed in 90g of deionized water to form a 10% PVA solution in a 90℃ water bath. After cooling to room temperature, the solution was centrifuged to remove insoluble matter, and a homogeneous, transparent mixture was collected. This mixture was then transferred to an -80℃ ultra-low temperature freezer and placed for 24 hours, followed by 4 hours at room temperature. This process was repeated three times to obtain the PVA hydrogel.

[0071] Infrared spectroscopy testing method: After freeze-drying, the infrared spectrum was obtained using the KBr pellet method. The infrared spectrum is shown below. Figure 1 As shown.

[0072] Figure 2 The transparency of the PCSA hydrogels prepared in Examples 1, 5 and 7 is demonstrated, and the leaves behind the hydrogels are clearly visible.

[0073] Mechanical properties of PCSA hydrogels prepared in Examples 1 to 7

[0074] PCSA hydrogel was prepared into dumbbell-shaped specimens (25 mm long × 10 mm wide × 4 mm thick) for tensile testing at a tensile speed of 100 mm / min. The strength at which the hydrogel breaks is recorded as the tensile strength of the hydrogel. PCSA hydrogel was also prepared into cylinders (10 mm diameter × 12 mm height) for compression testing at a compression speed of 50 mm / min. The strength of the hydrogel when compressed to 80% is recorded as the compressive strength of the hydrogel. Each sample was tested three times, and the average value was taken.

[0075] The tensile strength of the PCSA hydrogels prepared in Examples 1-7 is as follows: Figure 3 As shown in (a), the results indicate that the tensile strength of Example 5 reached 0.980 ± 0.039 MPa. The compressive strength is as follows... Figure 3 (b) The results show that the PCSA hydrogel has excellent mechanical properties, with the optimal compressive strength being 7.38 ± 0.15 MPa and the tensile and compressive strengths being stronger than those of Comparative Example 2 and Comparative Example 3.

[0076] Antibacterial activity of the PCSA hydrogels prepared in Examples 1, 5 and 7

[0077] Liquid culture medium preparation

[0078] The preparation method is as follows: Weigh 2.5g of tryptone powder, 1.25g of yeast extract powder and 2.5g of sodium chloride into a 500ml Erlenmeyer flask, add 250ml of pure water to dissolve, seal with sterile breathable sealing film and tie with rubber band, autoclave at 120℃ for 20min, and use after cooling to room temperature.

[0079] Preparation of solid culture medium

[0080] The preparation method is as follows: Weigh 2.5g of tryptone, 1.25g of yeast extract, 2.5g of sodium chloride, 3.7g of agar, and 250ml of water. Heat and dissolve the contents appropriately, then adjust the pH to neutral. Seal the contents with a heat-resistant, breathable sealing film and sterilize at 120℃ under high pressure for 20 minutes. After allowing it to cool naturally, spread the contents into petri dishes, 10ml per dish. Use the petri dish after it has completely cooled and solidified.

[0081] Preparation of bacterial suspension

[0082] Activated Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, when their OD values ​​measured at 600 nm using an ELISA reader are between 0.3 and 0.5, are inoculated into 4 ml of sterile liquid culture medium and 0.3 g of hydrogel and placed in a constant temperature shaker at 37°C for 3 h with shaking at 200 rpm.

[0083] Plate counting method

[0084] Take 10 μL of bacterial suspension from each sample and dilute it with 1 ml of PBS. Then, spread 100 μL of the diluted solution onto an agar plate and incubate for 12 hours. Once colonies have formed, photograph the viable colony units on the plate.

[0085] like Figure 4 As shown, the PCSA hydrogels obtained in Examples 1, 5 and 7 have excellent antibacterial properties.

[0086] Cell compatibility of PCSA hydrogels prepared in Examples 1, 5 and 7

[0087] Test method: The hydrogel was first irradiated with UV light for 1 hour, then flipped over and irradiated for another hour. 3000 human umbilical vein endothelial cells were seeded per well and co-cultured with the hydrogel for 24 hours, 48 ​​hours, and 72 hours. The absorbance (OD) at 450 nm was measured using a microplate reader according to the CCK8 kit instructions. Pure culture medium without hydrogel served as a control group.

[0088] from Figure 5It can be seen that at different time points, the OD values ​​of the PCSA hydrogels prepared in Examples 1, 5 and 7 are all higher than those of the control group and Comparative Examples 2 and 3, indicating that the hydrogels obtained in this invention have good cell compatibility.

[0089] Migration experiments of the PCSA hydrogels prepared in Examples 1, 5 and 7

[0090] The effect of PCSA hydrogels prepared in Examples 1, 5, and 7 on the in vitro migration of human umbilical vein endothelial cells was detected using a Transwell assay. 3 × 10⁻⁶ ppm of the hydrogels were added to the Transwell chambers. 5 Cells were cultured in two chambers. A sterile hydrogel and 550 μL of serum-containing complete culture medium were added to the lower chamber. After 24 h of co-culture, the lower chamber medium was aspirated, the chamber was washed with PBS, fixed at room temperature for 30 min, washed again, and then 0.8% crystal violet solution was added. Staining was performed at room temperature for 20 min, the crystal violet was discarded, the chamber was washed, and the cells were observed and photographed using an inverted microscope. 550 μL of serum-containing complete culture medium was added to the lower chamber as a control group.

[0091] Transwell experimental results are as follows: Figure 6 As shown, the PCSA hydrogels prepared in Examples 1, 5 and 7 are more conducive to the migration of human umbilical vein endothelial cells.

[0092] Angiogenesis-promoting ability of PCSA hydrogels prepared in Examples 1, 5 and 7

[0093] The angiogenesis assay is performed as follows: Dilute the matrix gel 1:3 with serum-free medium and add it to a pre-cooled 96-well plate at 80-100 μL / well. Incubate for 1 hour to allow gel formation. Resuspend HUVECs in culture medium or extract and incubate at 2 × 10⁻⁶ μL / well. 4 A concentration of / μl was added to a 96-well plate, and the plate was incubated at 37°C for 8 hours. Tube formation was then observed under a light microscope. The results of the angiogenesis experiment are as follows: Figure 6 As shown. Figure 7 As shown, the vascular ring density of the PCSA1, PCSA5, and PCSA7 hydrogel groups was better than that of the control group, indicating the excellent in vitro angiogenesis ability of PCSA hydrogel.

[0094] The PCSA hydrogels prepared in Examples 1 and 7 promoted wound healing in diabetic rats.

[0095] Establishing a diabetic rat model

[0096] This experiment selected 15 male SPF-grade SD rats weighing 250-300 kg at 6 weeks of age as subjects for the establishment of a diabetic mouse model. The streptozotocin was injected intraperitoneally at a dose of 60 mg / kg per rat, every other day for 4 consecutive weeks. The diabetic rat model was considered to have been successfully established when the tail vein blood glucose level was ≥16.67 mmol / L.

[0097] Repairing effect of PCSA hydrogel on skin defects in diabetic rats

[0098] Fifteen successfully modeled diabetic mice were used as experimental animals and randomly divided into three groups of five mice each using a random number table: PCSA0 group (control group), PCSA1 group, and PCSA7 group (experimental group). After deep anesthesia, all hair on the back of the mice was shaved, and the skin was disinfected with 75% ethanol. A full-thickness circular skin defect with a diameter of 15 mm was created using a piercing instrument to establish a skin injury model. Hydrogel was applied to the wound, and the wound healing process was observed and photographed at different time points.

[0099] Wound healing results as follows Figure 8 As shown. Figure 8 As shown, compared with the PCSA0 group, the PCSA1 and PCSA7 groups significantly accelerated the wound healing process. The PCSA7 hydrogel group was completely healed by day 12, demonstrating excellent ability to promote the healing of diabetic wounds.

[0100] The above tests and experiments demonstrate that the PCSA7 hydrogel prepared in this invention possesses tunable mechanical properties and excellent antibacterial and cell compatibility. It significantly promotes cell proliferation and in vitro angiogenesis of vascular endothelial cells, and also exhibits a significant wound-healing capacity in diabetic rat wound healing experiments. Due to the full integration of the bioactivities of CS and Curl, it plays a crucial role in antibacterial and angiogenesis promotion, facilitating the reconstruction of the wound microenvironment, rapidly restoring blood supply, and accelerating wound healing. The full integration of PCSA also enables the long-term, slow release of Curl from within the hydrogel, thus avoiding rapid Curl metabolism and preventing excessive release leading to high concentrations of Curl that could negatively impact cell activity at the wound site. This enhances the material's long-lasting activity and fully realizes its wound-healing potential.

[0101] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A traditional Chinese medicine hydrogel composition with antibacterial and angiogenesis-promoting properties, characterized in that, Includes the following components by weight: PVA: 8-10 parts; CS-SA-Cur pregel: 0.2~1.2 parts; Water: 90-100 parts; The CS-SA-Cur pregel comprises the following components in parts by weight: Cur: 0.1~0.3 parts; CS: 0.5~1.5 parts; SA: 0.5~1.5 parts; Alkali / urea mixture: 40-50 parts; The preparation method of the CS-SA-Cur pregel is as follows: CS and Cur were dispersed in an alkaline / urea aqueous solution, stirred, and sodium alginate was added. After mixing, the mixture was allowed to stand at -30~-10℃ for 12 h, thawed at room temperature, and then allowed to stand at -90~-70℃ for 24 h. After thawing at room temperature, this was recorded as one freeze-thaw cycle. After two freeze-thaw cycles, the mixture was centrifuged at room temperature, and the supernatant was collected to obtain the CS-SA-Cur pregel.

2. The antibacterial and angiogenesis-promoting traditional Chinese medicine hydrogel composition as described in claim 1, characterized in that, The alkali / urea mixture comprises lithium hydroxide, potassium hydroxide, urea, and water, wherein the weight ratio of lithium hydroxide, potassium hydroxide, urea, and water is 4.5:7:8:80.

5.

3. A method for preparing a traditional Chinese medicine hydrogel composition with antibacterial and angiogenesis-promoting properties as described in claim 1, characterized in that, Includes the following steps: S1. Dissolve PVA in deionized water at 90℃ to obtain a uniform and transparent PVA solution; S2. Add the CS-SA-Cur pregel to the PVA solution, stir and centrifuge to remove insoluble matter, freeze at -90~-70℃ for 24h, thaw at room temperature for 2~4h, and record it as one cycle. Repeat the cycle three times to obtain the antibacterial and angiogenesis-promoting traditional Chinese medicine hydrogel composition.

4. The use of the antibacterial and angiogenesis-promoting traditional Chinese medicine hydrogel composition as described in claim 1 in the preparation of wound healing materials.

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