一种用于脾氨肽分离提纯的功能化纳滤膜及其制备方法
By preparing a pH-responsive variable pore size nanofiltration membrane and constructing a nanofiltration membrane on a PVDF microporous membrane using a triblock copolymer, the problem of low separation and purification efficiency of spleen peptides in the prior art has been solved, achieving efficient separation of peptides and amino acids and improving the quality and efficacy of biopharmaceuticals.
CN117463174BActive Publication Date: 2026-07-17ZHEJIANG FENGAN BIOPHARM
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG FENGAN BIOPHARM
- Filing Date
- 2023-11-09
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies struggle to efficiently separate and purify the polypeptides and amino acids in spleen peptides, resulting in poor quality and efficacy of biopharmaceuticals.
Method used
A triblock copolymer polyisoprene-polystyrene-polyacrylic acid was prepared by controlled/living radical polymerization. The PI-PS-PAA triblock copolymer nanofiltration membrane was then constructed on a PVDF microporous membrane through chemical modification. This resulted in a pH-responsive variable pore size nanofiltration membrane, enabling multi-stage separation of peptides and amino acids from spleen peptides.
Benefits of technology
It increased the content of polypeptide active ingredients in spleen peptide, significantly improving the quality and efficacy of biological drugs.
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Figure CN117463174B_ABST
Abstract
本发明提供了一种用于脾氨肽分离提纯的功能化纳滤膜及其制备方法，以AIBN为引发剂，异戊二烯、苯乙烯、丙烯酸为反应单体，DMF为溶剂，通过三者之间发生共聚反应，得到三嵌段共聚物聚异戊二烯‑聚苯乙烯‑聚丙烯酸。再利用嵌段共聚物在溶液‑空气界面自组装成有序纳米孔结构，在PVDF中空纤维层上构筑PiP‑PSt‑PAA三嵌段共聚物纳滤膜，通过化学修饰反应将氨基烷基磺酸引入三嵌段共聚物中，制备新型基于pH响应的可变孔径纳滤膜。利用该新型pH响应型变孔径纳滤膜，分离脾脏浆液制得脾氨肽粉剂，将其应用于脾氨肽中多肽和氨基酸的高效多级分离，从而提高脾氨肽中多肽活性成分的含量，大大提高此类生物药物的质量和疗效。
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