A system and method for improving polymerase performance using hsp20

CN117467639BActive Publication Date: 2026-10-20INNOVATION ACAD FOR PRECISION MEASUREMENT SCI & TECH CAS
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Patent Information

Application Number
CN202311267379.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-28
Publication Date
2026-10-20
Estimated Expiration
2043-09-28

AI Technical Summary

Technical Problem

Pfu 聚合酶是在嗜热的古核生物火球菌属内发现的，该酶同时具有5'-3'聚合酶活性和3'-5 '外切核酸酶活性，在活体内进行DNA复制时，能在聚合反应中纠正错误掺入的碱基，保真性极高，然而，在体外PCR实验中，Pfu聚合酶的热稳定性较差，并且扩增长片段的能力不足，扩增效果不佳，不利于Pfu聚合酶的大规模推广

Benefits of technology

[0016]1、本发明一种使用Hsp20提高聚合酶性能的体系包括dNTP 150- 500μmol/L、Pfu聚合酶10-60U/mL、hsp20 0.1pg/uL-100ng/uL；该体系中添加有hsp20，hsp20为一种热稳定性蛋白，能够保证自身在高温条件下的稳定，同时还能显著提高Pfu聚合酶在高温体系下的稳定性（活性保留），进而增强Pfu聚合酶在高温下的扩增性能。因此，本发明所述体系能够显著提高Pfu聚合酶的热稳定性。

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Abstract

A system and method for improving polymerase performance using Hsp20, the system comprising dNTP 150-500 micromole / L, Pfu polymerase 10-60 U / mL, hsp20 0.1 pg / uL-100 ng / uL. The system adds hsp20, which is a heat-stable protein that can ensure its stability under high temperature conditions, and can also significantly improve the stability of Pfu polymerase under high temperature system, thereby enhancing the amplification performance of Pfu polymerase.
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Citation Information

Patent Citations

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  • Novel recombinant Pfu DNA polymerase

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