Method for enhancing the production of ansamitocin by overexpressing the ansamitocin in vivo target protein apasm_6307

By enhancing the expression of the in vivo target protein gene of anthracycline in the precious actinomycetes, the problems of intracellular toxicity and feedback inhibition of synthase during anthracycline fermentation were solved, resulting in a significant increase in anthracycline yield.

CN117467686BActive Publication Date: 2026-07-14SHANGHAI JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI JIAOTONG UNIV
Filing Date
2021-10-18
Publication Date
2026-07-14

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Abstract

The application discloses a method for enhancing actinomycin production by enhancing expression of an actinomycin in-vivo target protein APASM_6307. The method comprises the following steps: enhancing expression of the actinomycin in-vivo target protein APASM_6307 by using a strong promoter kasOp* in Actinocatenulum praeciosum ATCC 31280, and obtaining a high-yield actinomycin mutant strain HQG-04. Compared with a starting strain, the high-yield strain HQG-04 obtained by the method has a fermentation yield increased by 93.5%, and a laboratory shake flask fermentation level reaches 89 mg / L.
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