Sample detection device and detection method thereof

Abstract

The application provides a sample detection device and a detection method thereof; the sample detection device is a one-piece sealed device, which can prevent interference of external substances and degradation of samples during open detection, improve detection accuracy and precision, and provide more reliable detection results; through matching design of two bottle caps, control of liquid in the device under full sealing condition is realized; the design of moving of the test strip can control the amount of sample processing liquid contacted by the test strip, and avoids problems of false negative and false positive caused by low concentration and high concentration samples; the design of the scraping rib helps to separate the to-be-detected liquid from the sampling part, and improves the efficiency of sample processing; the detection steps are convenient and portable, the device can realize detection anytime and anywhere, has no professional requirements for the user, and is convenient for various people to obtain effective detection results according to needs.

Description

[0001] This application claims priority to Chinese prior application, application number 202311138617.5, filed on September 5, 2023, the description, claims, abstract and drawings of which are incorporated herein by reference in their entirety. Technical Field

[0002] This invention belongs to the field of medical devices, and in particular relates to a sample detection device and its detection method. Background Technology

[0003] Sample testing is an important tool in fields such as biomedicine, environmental monitoring, and chemical analysis. It typically involves mixing the sample solution with one or more liquids and then detecting the sample using instruments or test strips. However, most existing sample testing devices are bulky and inconvenient to carry. Furthermore, they require significant time and resources for sample collection, processing, transfer, and cleaning before and after testing, which is not only inefficient but also prone to sample contamination or loss.

[0004] To address the aforementioned issues, some portable sample detection devices have been developed. For example, CN111269825A discloses a rapid nucleic acid POCT integrated microfluidic reagent kit, its preparation method, and its application, which integrates nucleic acid extraction, amplification, and detection functions, achieving closed-tube integrated nucleic acid extraction, amplification, and detection. However, the aforementioned portable sample detection devices still have some shortcomings: on the one hand, the box or mounting base needs to be opened and closed multiple times during collection and detection, which can easily lead to leakage or evaporation of sample liquid or mixture; on the other hand, when detecting mixtures, they need to be transferred to instruments or test strips, which not only increases the number of operating steps and consumable costs but may also affect the accuracy and sensitivity of the detection.

[0005] Therefore, there is an urgent need to develop a one-piece, enclosed, portable sample detection device to address the requirements for detecting sample liquids and mixtures of two liquids, in order to solve the problems in existing technologies. Summary of the Invention

[0006] To address the aforementioned issues, this invention provides a sample detection device and its detection method. This device is a one-piece, enclosed, portable sample detection device that meets the requirement of mixing two liquids with the sample for processing. From the detection results, this design improves the accuracy and precision of the detection, providing more reliable results.

[0007] The sample detection device provided by this invention achieves control over the liquid inside the device in a fully enclosed state through the matching design of two bottle caps, which is beneficial to the detection. The design of the test strip movement can control the amount of sample processing liquid that the test strip contacts, avoiding false negatives and false positives caused by low-concentration and high-concentration samples, making the detection results more reliable. The design of the scraper helps to separate the test liquid from the sampling component, improves the efficiency of sample processing, and increases the accuracy and precision of sample detection.

[0008] The sample testing device provided by this invention has convenient and portable testing steps, enabling testing anytime and anywhere. It does not require professional skills from users, making it convenient for various groups of people to obtain effective test results according to their needs.

[0009] On one hand, the present invention provides a sample detection device, including a sample collection component, a sample processing component, and a sample detection component; the sample processing component contains a sample processing solution, and the sample collected by the sample collection component is processed by the sample processing solution to obtain a sample solution to be tested; the sample processing solution includes solution A and solution B; the sample processing component includes a second cap, and solution B flows out when the second cap is closed; the sample detection component is used to detect the sample solution to be tested; and the sample collection component is used to collect the sample.

[0010] The sample testing device also includes a bottle body, which is integrally molded. This body provides support for the sample processing and testing components and also acts as a seal. This integrated operation avoids errors caused by human operation, improves the reliability of test results, and makes the testing method more convenient. The sample processing component processes the sample inside the bottle body. Closing the second cap separates the sample from the sample collection component, allowing solution B to flow out without additional steps. The sample testing component can test the mixed solution of the sample, solution A, and solution B. The sample collection component can transfer a sufficient amount of the collected sample to the sample processing component, awaiting further processing and testing.

[0011] In some embodiments, the second cap includes a cap and a lever, the lever being fixed below the cap; liquid B is contained in an inverted reagent bottle containing a first cap, and when the second cap is applied, the lever pushes the first cap away from the reagent bottle.

[0012] In some embodiments, the rod is provided with a first gear and the second bottle cap is provided with a second gear; the second gear can cooperate with the first gear, and when the second bottle cap rotates, it can drive the rod to rotate, thereby driving the second gear to rotate, and at the same time pushing the first gear to rotate, so that the first bottle cap is detached from the reagent bottle, and the B solution in the reagent bottle enters the sample processing component.

[0013] The second gear and the first gear are also positioned in a coordinated manner: throughout the entire rotation process, the height variation range of the second gear is sufficient to allow it to move in conjunction with the first gear; the size and distance of the gears are coordinated to ensure that the transmission between the gears can be fully carried out; when the second cap is fully tightened or before it is fully tightened, the first cap is rotated to detach from the reagent bottle, allowing liquid B to flow out.

[0014] Furthermore, the sample processing component is provided with a first tank, a second tank, and a sample inlet; the first tank is located below the test strip and is used to contain liquid A; the second tank is used to place a second bottle cap; before sample detection, the second bottle cap is located in the second tank; when the sample collection component collects the sample and places it in the sample processing component, the second bottle cap is removed from the second tank and placed on the sample inlet.

[0015] Furthermore, the sample processing component is equipped with a sampling pool, in which the sample is separated from the sample collection component; the sampling pool is connected to the first tank, and the sample is mixed with liquid A in the first tank.

[0016] Furthermore, the sample processing component is equipped with a strip-shaped scraper, which helps separate the sample from the swab when the swab is rotated.

[0017] The sampling pool is a vertical channel with a small diameter, slightly larger than the diameter of the swab rod but smaller than the cross-sectional diameter of the swab head when relaxed. This ensures that the swab head is compressed within the sampling pool while the swab rod can move freely up and down and rotate. Due to the compression from the inner wall of the sampling pool and the compression from the strip scraper during rotation, the liquid to be tested will be more fully separated from the swab head and flow downwards along the inner wall of the sampling pool. The bottom of the sampling pool is connected to the first tank, and the liquid to be tested flows through the inner wall of the sampling pool into the first tank to mix with liquid A.

[0018] Furthermore, after liquid B flows out, it passes through the sample processing unit and mixes with liquid A and the sample in the first tank.

[0019] Because of the design provided by this invention, the sample is first separated from the swab head in the sampling pool and enters the first tank. After this step is completed, the swab rod is broken and removed before the second cap is placed on the inlet. Through the interaction of gears and other means, the first cap falls and liquid B flows out. Therefore, it can be ensured that the mixing order of the three liquids is: liquid A is mixed with the sample, and the mixture of liquid A and the sample is then mixed with liquid B. This design can avoid the situation where the detection fails due to the order of liquid mixing.

[0020] Furthermore, the sample detection component includes a test strip, a test strip frame, a test strip channel, and a base; the test strip is mounted on the test strip frame; the test strip frame can carry the test strip and move within the test strip channel; before sample detection, the test strip frame is separated from the base; when sample detection is performed, the test strip frame is combined with the base and moved to the sample liquid to be tested for sample detection.

[0021] Furthermore, the base is located at the lower end of the test strip channel, which serves to seal the test strip channel and prevent liquid A from entering the test strip channel.

[0022] Furthermore, the sample collection component is a cotton swab, which includes a swab head and a swab stem. The swab head is used to collect samples. The swab stem is provided with a point to help break it. After the sample is collected, the sample collection component is inserted into the sample processing component from the sample inlet, the swab stem is broken off from the point and removed, and then the second cap is taken out from the second groove and placed on the sample inlet.

[0023] Furthermore, the sample detection device is provided with a test strip channel entrance at the top; the swab rod can enter the test strip channel entrance, thereby pushing the test strip frame downward to perform sample detection.

[0024] On the other hand, the present invention provides a method for sample detection using the above-described sample detection device, comprising the following steps:

[0025] (1) Samples were taken using cotton swabs;

[0026] (2) Open the inlet of the sample testing device and insert the cotton swab into the sample testing device through the inlet;

[0027] (3) Break off the swab stick and remove it, leaving the swab head inside the sample detection device;

[0028] (4) Take out the second bottle cap from the second tank below the sample detection device, put it on the sampling port and tighten the second bottle cap;

[0029] (5) Insert the broken cotton swab into the test strip channel entrance above the sample testing device and push the test strip downwards until it can no longer be pushed;

[0030] (6) After the test strip is completed, take out the test strip and read the test result.

[0031] The present invention has the following beneficial effects:

[0032] 1. The sample detection device provided by the present invention is an integrally formed sealed device, which can eliminate the interference of foreign substances and the degradation of samples during open testing, improve the accuracy and precision of the detection, and provide more reliable test results.

[0033] 2. The sample detection device provided by the present invention achieves control of the liquid inside the device in a fully enclosed state through the matching design of the first and second bottle caps. This avoids the steps of weighing and pouring liquid as in conventional detection methods, shortens the detection steps, avoids errors introduced by manual operation, and improves detection accuracy.

[0034] 3. The sample detection device provided by the present invention includes a design for moving the test strip, which can control the amount of sample processing liquid that the test strip contacts, avoiding false negatives and false positives caused by low-concentration and high-concentration samples, and making the test results more reliable.

[0035] 4. The sample detection device provided by the present invention includes a scraper design, which helps to separate the liquid to be tested from the sampling component, improves the efficiency of sample processing, and increases the accuracy and precision of sample detection.

[0036] 5. The sample testing device provided by this invention has convenient and portable testing steps, enabling testing anytime and anywhere. It does not require professional expertise from users, and various groups of people can easily obtain effective test results using the sample testing device provided by this invention according to their different needs. Attached Figure Description

[0037] Figure 1 Front view of the sample detection device

[0038] Figure 2 Right view of the sample detection device

[0039] Figure 3 Bottom view of the sample detection device

[0040] Figure 4 Top view of the sample detection device

[0041] Figure 5 Cross-sectional view of the sample detection device

[0042] Figure 6 Enlarged cross-sectional view of the sample detection device

[0043] Figure 7 Cross-sectional view of the sample separation device during sample detection.

[0044] Figure 8 3D diagram of the sampling pool and second tank of the sample detection device

[0045] Figure 9 3D diagram of the second bottle cap of the sample detection device

[0046] Figure 10 3D diagram of the reagent bottle for the sample testing device

[0047] Figure 11: Three-dimensional cross-sectional view of the sample detection component of the sample detection device

[0048] Figure 12 3D diagram of the sample acquisition device of the sample detection device

[0049] Figure 13 Schematic diagram of gear engagement in sample testing device Detailed Implementation

[0050] The preferred embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. It should be noted that the following embodiments are intended to facilitate the understanding of the present invention and are not intended to limit it in any way. All features disclosed in the embodiments of the present invention, or all steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any way.

[0051] Example 1: Sample Detection Device

[0052] The sample detection device provided by this invention is as follows: Figures 1 to 7 As shown, Figure 1 This is a front view of the sample detection device. Figure 2 Side view of the sample detection device. Figure 3 This is a bottom view of the sample detection device. Figure 4 This is a top view of the sample detection device. Figure 5 This is a cross-sectional view of the sample detection device. Figure 6 This is an enlarged cross-sectional view of the sample detection device. Figure 7 This is a cross-sectional view of the sample detection device during testing.

[0053] The sample detection device 1 provided by the present invention includes a bottle body 2, a sample processing component 3, a sample detection component 4, and a sample collection component 5. The sample processing component 3 and the sample detection component 4 are fixed in the integrally formed bottle body 2.

[0054] like Figure 1 , Figure 2 , Figure 3As shown, the sample detection device 1 includes a bottle body 2 and a sample processing component 3. The bottle body 2 is integrally formed. The sample processing component 3 includes a second bottle cap 312, which is screwed onto the bottle body 2 and accommodated in a second groove 300. The second groove 300 is not connected to the internal space formed by the bottle body 2. The second bottle cap 312 has a cover 313 at its lower part, which can be rotated to remove the second bottle cap 312 from the bottle body 2. The top of the sample detection device 1 also has a sealing cap 301, which is screwed onto the sample inlet 303 and fixed to the bottle body 2. The screw connecting the sealing cap 301 to the bottle body 2 is exactly the same as the screw connecting the second bottle cap 312 to the bottle body 2. After the second bottle cap 312 is removed from the bottle body 2, it can be screwed onto the bottle body 2 at the position of the sealing cap 301. Whether the second bottle cap 312 or the sealing cap 301 is screwed onto the sample inlet 303, the interior of the sample detection device 1 is completely sealed.

[0055] like Figure 4 As shown, the sample detection device 1 also includes a sample detection component 4, which includes a test strip channel inlet 401 located on the upper part of the cup shoulder, next to the sample inlet 303.

[0056] like Figure 5 As shown, the sealing cap 301 of the sample detection device 1 has a matching spiral structure with the inlet 303, and the spiral 302 of the inlet 303 also matches the spiral 311 of the second cap 312; rotating the cap 313 can detach the second cap 312 from the bottle body 2; the second cap 312 includes a rod 310 and a cap 313, and a second gear 309 is provided on the rod 310. When the second cap 312 is removed, it is screwed into the bottle body 2 from the inlet 303. The height of the second gear 309 on the rod 310 matches the first gear 307 on the first cap 306, and they are positioned close together. The gear structures of the second gear 309 and the first gear 307 are also matched. When the rod 310 of the second cap 312 is rotated, the second cap 312 is detached from the bottle body 2. When the rod 310 is inserted into the bottle body 2, the second gear 309 and the first gear 307 can mesh with each other. Rotating the cap 313 of the second cap 312 causes the spiral structure 311 on the cap 313 to connect with the spiral 302 on the injection port 303. At this time, the rod 310 also rotates. The second gear 309 on the rod 310 drives the first gear 307 to rotate, which in turn causes the first cap 306 to rotate. It is worth noting that the spiral structure connecting the first cap 306 to the bottle body 2 has a spiral length equal to or less than the spiral length of the spiral 302. This causes the first cap 306 to be unscrewed and separated from the bottle body 2 when the second cap 312 is tightened, and then falls onto the inclined surface 308.

[0057] The top of the first cap 306 is flat. When the first cap 306 is screwed onto the bottle body 2, this flat surface and the bottle body 2 form a reagent bottle 304. The reagent bottle has a cavity 305, which is used to contain liquid B. Liquid B is a liquid required for testing. Obviously, when the first cap 306 falls and lands on the inclined surface 308, liquid B in the reagent bottle 304 will flow out and onto the inclined surface 308. The inclined surface 308 is an inclined liquid flow channel, and liquid B will flow further downward along the inclined surface 308.

[0058] like Figure 6 As shown, when liquid B flows down to the bottom along the inclined plane 308, it enters the funnel 314 and continues to flow down along the funnel 314. The funnel 314 is a liquid flow channel in a funnel, connected to the inclined plane 308 at the top and to the sampling pool 315 at the bottom. After flowing to the bottom, it enters the sampling pool 315. The sampling pool 315 is a vertical liquid flow channel with one or more scraper ribs 316 inside. The scraper ribs 316 are long strip-shaped structures that protrude from the top to the bottom of the sampling pool 315.

[0059] like Figure 7 As shown, the sample collection component 5 can be a cotton swab, including a swab head 501 and a swab shaft 502. After sampling the biological sample, the swab head 501 should contain a certain amount of the liquid to be tested. The sealing cap 301 is unscrewed, and the cotton swab 5 is vertically inserted into the bottle body 2 through the inlet 303. The cross-sectional area of ​​the top of the funnel 314 is equal to or slightly larger than the inlet 303, allowing the vertically inserted cotton swab 5 to enter the sampling pool 315 under the guidance of the funnel 314. The cross-sectional area of ​​the sampling pool 315 is slightly larger than the cross-sectional area of ​​the swab shaft 502, but smaller than the cross-sectional area of ​​the swab head 501 when relaxed, allowing the swab shaft 502 to be freely inserted and rotated. The swab head 501... When squeezed in the sampling pool 315, the liquid to be tested in the swab head 501 is separated from the swab head 501. It is worth noting that, in order to make the separation of the liquid to be tested more thorough, the sampling pool 315 is equipped with scraping ribs 306. When the swab rod 502 is rotated and rubbed, the scraping ribs 306 further squeeze the swab head 501, causing the liquid to be tested to be further separated and enter the sampling pool 315, and flow vertically downward. The swab 5 is easy to break in the middle. After completing the above steps, the swab 5 can be broken in the middle, into the swab head 501 and the swab rod 502. The swab head 501 cannot be removed and remains in the sampling pool 315, while the swab rod 502 can be removed for further operations.

[0060] We go back Figure 5 , Figure 5The sample detection device 1 also showcases a sample detection component 4, which includes a test strip channel inlet 401, a test strip 402, a test strip frame 403, a test strip channel 404, a base 405, and a first tank 406. The first tank 406 contains liquid A, which is another liquid used for detection. The first tank 406 is connected to the bottom of the sampling pool 315, allowing both the test liquid and liquid B to enter the first tank 406 from the bottom of the sampling pool 314 and mix with liquid A. The diameter of the test strip channel inlet 401 is slightly larger than that of the swab rod 502, allowing the swab rod 502 to be inserted downwards from the test strip channel inlet 401. The test strip 402 can detect the test substance. The test strip 402 is mounted on the test strip frame 403 and can move with the test strip. The test strip frame 403 moves up and down, and the base 405 is engaged with the lower end of the test strip channel 404, separating the test strip channel 404 from the first groove 406, thus preventing liquid in the first groove 406 from entering the test strip channel 404 before testing. The base 405 is separate from the test strip frame 403, but when pushed downward by the swab rod 502, the test strip frame 403 moves downward, encounters the base 405, and pushes the base 405 downward together, finally contacting the first groove 406 and the liquid therein. After the base 405 contacts the bottom of the bottle body 2, it will also provide a certain height. The final height of the test strip 402 entering the first groove 406 is fixed, so that the contact amount between the test strip 402 and the mixed liquid in the first groove 406 is controllable.

[0061] like Figure 8 As shown, the sampling pool 315 is equipped with scraping ribs 316, which can be multiple vertical strip-shaped scraping ribs. When the cotton swab rod 502 is rotated and rubbed, the scraping ribs 306 further squeeze the cotton swab head 501, so that the liquid to be tested is fully separated and enters the sampling pool 315, and flows vertically downward. The liquid to be tested flows downward to the bottom 317 of the sampling pool, and the bottom 317 of the sampling pool is connected to the first tank 406, so that the liquid to be tested can flow into the first tank and mix with liquid A.

[0062] like Figure 9 As shown, the second bottle cap 312 includes a cap 313 and a rod 310, the rod 310 having a second gear 309; as Figure 10As shown, reagent bottle 304 has a first cap 306. Both reagent bottle 304 and first cap 306 are inverted inside sample detection device 1. First cap 306 has a first gear 307. First gear 309 and second gear 307 cooperate with each other. This design allows the second gear 309 of rod 310 to rotate when the second cap 312 is screwed into the sample inlet 303, which in turn drives the first gear 307 to rotate. When the second cap 312 is tightened in the sample inlet 303, the first cap 306 rotates in the opposite direction and is driven to rotate and fall off reagent bottle 304, causing liquid B to flow out. This design is more ingenious, as it allows control of the liquid inside sample detection device 1 without additional operation, avoiding the detection steps of weighing and pouring liquid in conventional detection methods, and also avoiding unnecessary errors introduced by manual operation, thus improving the accuracy of detection.

[0063] Sample detection component 4 includes a test strip channel inlet 401, a test strip 402, a test strip frame 403, a test strip channel 404, a base 405, and a first groove 406, as shown below. Figure 11 As shown, the test strip frame 403 has a circular groove 407 at its upper end. Initially, when no test is being performed, the circular groove 407 is at the top of the test strip channel 404, close to the test strip channel inlet 401. The diameters of the circular groove 407 and the test strip channel inlet 401 are slightly larger than the diameter of the sampling rod 502, allowing the swab rod 502 to be freely inserted downwards from the test strip channel inlet 401, pushing the circular groove 407, and thus pushing the test strip frame 403 downwards. The base 405 is a fixed object with slightly high frictional damping. When not subjected to external force, it can be stuck at the bottom of the test strip channel 404 by its own damping, thus isolating the first groove 406 from the test strip channel 404. During the action of external force, i.e., the swab rod 502 is inserted downward into the circular groove 407 from the test strip channel entrance 401, which in turn pushes the test strip frame 403 downward, the base 405 is pushed and continues to move downward until it touches the bottom of the first groove 406 and can no longer move downward. At this time, the test strip 402 also moves downward with the test strip frame 403 and comes into contact with the mixed solution in the first groove 46 for detection. The base 405 provides a certain height for the test strip 402, controls the volume of the mixed solution that the test strip 402 contacts, and avoids false negatives or false positives caused by the test strip 402 contacting too much or too little mixed solution, thereby improving the reliability of the test results.

[0064] Sample collection component 5 can be a cotton swab, such as... Figure 12As shown, the cotton swab 5 includes a swab head 501, a swab stem 502, and a sampling point 503. After the swab head 501 separates the liquid to be tested in the sampling pool 315, the swab head 501 does not need to be removed and can be easily broken off from the sampling point 503, separating the swab head 501 from the swab stem 502. The swab stem 502 is reserved for use in the next step of testing. This design allows the use of only the cotton swabs provided by the sample testing device of this invention, making the operation more convenient.

[0065] Figure 13 The entire process is shown, from the removal of the second cap 312 from the second tank 300, to its placement on the injection port 303, to its rotation inward, to the first cap 306 falling off due to the interaction of the first gear 307 and the second gear 309.

[0066] Therefore, the detection process of sample detection device 1 is as follows:

[0067] (1) To collect biological samples, the cotton swab tip 501 of the cotton swab 5 will have the liquid to be tested on it.

[0068] (2) Unscrew the sealing cap 301, insert the cotton swab 5 with the cotton swab head 501 facing down vertically into the bottle body 2 from the sample inlet 303, and finally reach the sampling pool 315 to separate the liquid to be tested from the cotton swab head 501. The liquid to be tested flows down the inner wall of the sampling pool 315 and enters the first tank 406, where it mixes with the liquid A stored in the first tank 406. Break the cotton swab 5 and take out the cotton swab rod 502 for later use.

[0069] (3) Rotate the cap 313 to remove the second cap 312 from the second tank 300. Place the second cap 312 in the injection port 303 and tighten it. The second gear 309 on the rod 310 of the second cap 312 will drive the first gear 307 to rotate, causing the first cap 306 to fall down. The B solution in the reagent bottle 304 will flow out and flow into the first tank 406 along the inclined surface 308, funnel 314, and sampling pool 315, and mix with the test liquid and A solution.

[0070] (4) Insert the cotton swab rod 502 downward from the test strip channel inlet 401, push the test strip 402 downward, and the test strip 402 comes into contact with the mixed liquid in the first tank 406;

[0071] (5) After a period of time, take out the test strip 402 and observe the test results.

[0072] The application of this invention is not limited to this. It can be extended to other applications, such as reagent detection. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of this invention; therefore, the scope of protection of this invention should be determined by the scope defined in the claims.

Claims

1. A sample detection device, characterized in that, It includes sample collection components, sample processing components, and sample detection components; The sample processing component contains a sample processing solution. The sample collected by the sample collection component is processed by the sample processing solution to obtain a test sample solution. The sample processing solution includes solution A and solution B. The sample processing component includes a second cap, which allows solution B to flow out when the second cap is closed. The sample detection component is used to detect the sample liquid to be tested; The sample collection component is used to collect samples; The second cap includes a cap and a rod, with the rod fixed below the cap; liquid B is contained in an inverted reagent bottle containing a first cap, and when the second cap is put on, the rod pushes the first cap away from the reagent bottle; The rod is equipped with a first gear, and the second bottle cap is equipped with a second gear. The second gear can cooperate with the first gear. When the second bottle cap rotates, it can drive the rod to rotate, thereby driving the second gear to rotate, and at the same time pushing the first gear to rotate, so that the first bottle cap is separated from the reagent bottle, and the B solution in the reagent bottle enters the sample processing component. The sample processing unit is provided with a first tank, a second tank, and a sample inlet; the first tank is located below the test strip and is used to contain liquid A; the second tank is used to place a second bottle cap; before sample detection, the second bottle cap is located in the second tank; when the sample collection unit collects the sample and places it in the sample processing unit, the second bottle cap is removed from the second tank and placed on the sample inlet.

2. The sample detection device as described in claim 1, characterized in that, The sample detection component includes a test strip, a test strip frame, a test strip channel, and a base; the test strip is mounted on the test strip frame; the test strip frame can carry the test strip and move within the test strip channel; before sample detection, the test strip frame is separated from the base; when sample detection is performed, the test strip frame is combined with the base and moved to the sample liquid to be tested for sample detection.

3. The sample detection device as described in claim 2, characterized in that, The base is located at the lower end of the test strip channel and serves to seal the test strip channel, preventing liquid A from entering the test strip channel.

4. The sample detection device as described in claim 3, characterized in that, The sample collection component is a cotton swab, which includes a swab head and a swab stem. The swab head is used to collect samples. The swab has a point on it to help break it. After the sample is collected, the sample collection component is inserted into the sample processing component from the inlet, the swab stem is broken off from the point and removed, and then the second cap is taken out from the second tank and placed on the inlet.

5. The sample detection device as described in claim 4, characterized in that, The sample testing device has a test strip channel entrance at the top; the cotton swab can enter the test strip channel entrance and push the test strip frame down to perform sample testing.

6. The sample detection device as described in claim 5, characterized in that, The sample processing component is equipped with a sampling pool, in which the sample is separated from the sample collection component; the sampling pool is connected to the first tank, in which the sample is mixed with liquid A.

7. The sample detection device as described in claim 6, characterized in that, The sample processing component is equipped with a strip-shaped scraper. When the swab is rotated, the scraper helps to separate the sample from the swab.

8. The sample detection device as described in claim 7, characterized in that, After liquid B flows out, it passes through the sample processing unit and mixes with liquid A and the sample in the first tank.

9. A method for sample detection using the sample detection device as described in any one of claims 1 to 8, characterized in that, Includes the following steps: (1) Sampling was performed using cotton swabs; (2) Open the inlet of the sample testing device and insert the cotton swab into the sample testing device through the inlet; (3) Break off the swab and remove it, leaving the swab head inside the sample detection device; (4) Take out the second bottle cap from the second tank below the sample detection device, put it on the sampling port and tighten the second bottle cap; (5) Insert the broken cotton swab into the test strip channel entrance above the sample testing device and push the test strip downwards until it can no longer be pushed; (6) After the test strip is completed, take out the test strip and read the test result.

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