Hair growth and hair loss prevention using kaempferia oil
By using cinnamic acid esters, olefin compounds, and other components extracted from galangal essential oil, hair growth and hair loss prevention products are prepared, solving the problem of side effects of existing chemical drugs and achieving a purely natural and effective hair growth and hair loss prevention effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGDONG YUANSI SOUTH PHARM BIOTECHNOLOGY CO LTD
- Filing Date
- 2023-12-20
- Publication Date
- 2026-07-14
AI Technical Summary
Existing chemical drugs for treating hair loss have side effects, and current research has not fully utilized the hair growth potential of galangal essential oil, has not clarified the types and contents of its volatile components, and lacks effective natural hair loss prevention and hair growth products.
Using galangal essential oil as the main component, cinnamic acid esters, olefin compounds, borneol, and sitosterol are extracted by steam distillation or supercritical carbon dioxide extraction to prepare a skin application composition for hair growth and hair loss prevention. The addition amount is 0.1%-20%, and it is used in pharmaceutical preparations and daily chemical products.
It provides a pure, natural, and safe hair growth and anti-hair loss product that promotes the proliferation of hair papilla cells, regulates hair growth-related cytokines, significantly improves the hair growth cycle, and has a good effect on hair growth and anti-hair loss.
Smart Images
Figure CN117482189B_ABST
Abstract
Description
Technical Field
[0001] This application relates to the application of plant essential oils, particularly the use of galangal essential oil for hair growth and hair loss prevention, and its further application in pharmaceutical preparations or daily chemical products. Background Technology
[0002] Hair loss is a common skin condition, clinically divided into physiological and pathological hair loss. Physiological hair loss refers to the natural shedding of healthy hair during the telogen phase, also known as telogen effluvium, and generally requires no special treatment. Pathological hair loss refers to abnormal or excessive hair loss, also known as non-physiological hair loss, with androgenetic alopecia (also known as seborrheic alopecia) being the most common type. While it may not be a life-threatening disease, it significantly impacts a person's mental health and overall quality of life. Due to various factors such as environmental pollution, drug side effects, and social stress, an increasing number of men and women are suffering from hair loss or thinning hair. Data from the National Health Commission shows that in 2020, the number of men and women with hair loss in China was 164 million and 87 million respectively, with a significantly higher proportion of male hair loss than female hair loss, and the trend is showing a younger age of onset. For decades, the market for cosmetics and pharmaceuticals used for hair regrowth and hair loss prevention has been growing significantly. Currently, the clinically accepted treatments are minoxidil and the 5α-reductase inhibitor finasteride, but these drugs all have varying degrees of adverse side effects, such as sexual dysfunction, muscle growth disorders, hirsutism, local irritation, and erythema. Therefore, there is an urgent need for other effective treatment options, and natural products are an effective and abundant source for developing new hair loss prevention and hair regrowth agents.
[0003] Traditional Chinese medicine (TCM) has a long history of treating hair loss and plays an important role in traditional medicine. TCM herbs used to prevent and treat hair loss mainly fall into three categories: tonifying, clearing heat, and promoting blood circulation. Representative tonifying herbs include Polygonum multiflorum and ginseng; heat-clearing herbs include Sophora flavescens and Scutellaria baicalensis; and blood-activating herbs include Carthamus tinctorius. These herbs primarily regulate the cyclical pattern of hair growth and loss by modulating the levels of hormones, growth factors, and cytokines.
[0004] Kaempferia galanga, also known as sand ginger, is the rhizome of the plant *Kaempferia galanga* Linn., belonging to the ginger family (Zingiberaceae). It is distributed in Guangdong, Guangxi, Yunnan, and Taiwan. According to the *Chinese Pharmacopoeia* (2020 edition), the rhizome is pungent and warm in nature, entering the spleen and stomach meridians. It has the effects of regulating qi and warming the middle jiao, promoting digestion, and relieving pain. It also has a unique aroma and can be used as a flavoring spice. It is one of the "medicine and food homology" medicinal materials. In addition, Kaempferia galanga has been frequently used externally in ancient prescriptions for beauty and teeth cleaning. Modern domestic and international research has shown that it has pharmacological activities in skin care, such as promoting wound healing, inhibiting pigmentation, and providing sun protection. It can also dilate blood vessels, which may have a certain effect on promoting hair growth. It is a medicinal material with great application potential.
[0005] Current published articles and patents on the activity of galangal mainly focus on: the antibacterial, anti-inflammatory, antioxidant, anti-cancer, and nematicidal activities of its volatile components, as well as the antioxidant, antitumor, anti-infective, and lipid-lowering effects of flavonoids such as kaempferol and kaempferol. Existing patents include "A ginger family composition for dandruff removal and hair loss prevention, and its preparation method and application" (patent number CN 116035988A), which applies flavonoid components of galangal to dandruff removal and hair loss prevention; and "A hair-darkening and conditioning essential oil and its preparation method" (patent number CN 110037946A), which mentions that the tannic acid components of galangal essential oil can protect hair. However, existing studies have not added galangal as a main ingredient in hair care and hair loss prevention products; the content is low and the specific components are unclear. The chemical composition or its structural type is directly related to its activity; the active components in these formulations are flavonoids or tannic acid compounds; and the mechanism by which galangal exerts its hair-growth effect is not emphasized or specifically explained. Summary of the Invention
[0006] This application provides an embodiment of the use of galangal essential oil for hair growth and hair loss prevention, in order to solve the problems existing in related technologies. The technical solution is as follows:
[0007] This application provides an example of the use of galangal essential oil in the preparation of skin application compositions.
[0008] In one embodiment, the skin application composition is a hair growth and hair loss prevention drug.
[0009] In one embodiment, the skin application composition is used to promote hair growth.
[0010] In one embodiment, promoting hair growth includes promoting the proliferation of human dermal dermal papilla cells, and / or regulating the levels of hair growth-related cell growth factors, and / or improving the hair growth cycle.
[0011] In one embodiment, the preparation method of the galangal essential oil includes steam distillation or supercritical carbon dioxide extraction.
[0012] In one embodiment, the total content of cinnamic acid esters, olefin compounds, borneol and sitosterol in the kaempferol essential oil accounts for more than 90% of the total mass of the kaempferol essential oil.
[0013] In one embodiment, the skin application composition is applied to a pharmaceutical preparation or a daily chemical product.
[0014] In one embodiment, the pharmaceutical preparation includes patches, tinctures, creams, ointments, gels, and sprays.
[0015] In one embodiment, the daily chemical products include shampoo, conditioner, anti-hair loss treatment liquid, hair oil, hair care essence, hair oil, hair lotion, scalp and hair nourishing products, hair essence, scalp cream, hair gel, hair spray, hair mask, and eyebrow care liquid.
[0016] In one embodiment, the galangal essential oil is added in pharmaceutical preparations or daily chemical products at a mass percentage of 0.1%–20%.
[0017] The advantages or beneficial effects of the above technical solutions include at least the following:
[0018] (1) This application provides a pure natural galangal essential oil without organic solvent residue, and the raw materials of the essential oil are abundant, easy to obtain, and belong to medicinal and edible materials.
[0019] (2) This application provides a galangal essential oil with hair growth and anti-hair loss effects, including active ingredients such as cinnamic acid esters, olefin compounds, borneol, and sitosterol; its essential oil and active ingredients have few potential toxic side effects, the essential oil is conducive to skin penetration, and it has excellent effects in in vitro and in vivo experiments. It can be used to prepare daily chemical products that promote hair growth, strengthen hair or prevent hair loss.
[0020] (3) This application provides a daily chemical product containing galangal essential oil and used for hair growth and anti-hair loss effects, including but not limited to shampoo products, anti-hair loss care liquid, etc., wherein the amount of galangal essential oil added is 0.1-20% of the total mass of the daily chemical product, which can play a good role in maintaining scalp health, preventing hair loss and nourishing hair.
[0021] (4) Unlike the prior art, this application clarifies the types and contents of volatile components contained in galangal essential oil. Galangal essential oil contains a mixture of cinnamic acid esters, olefin compounds, borneol, sitosterol and other effective volatile components, and the total content of these components is more than 90% of the essential oil. Galangal essential oil does not contain flavonoids or tannic acid compounds. This is the difference between this patent and existing patents (such as above, a ginger family composition for dandruff prevention and hair loss prevention and its preparation method and application, patent number CN116035988A). For the first time, galangal essential oil and its main components are used for active research and application in hair growth and hair loss prevention, verifying the hair growth and hair loss prevention effects of galangal essential oil composed of the above components at the cellular and animal levels.
[0022] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of this application will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description
[0023] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the various drawings denote the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings depict only some embodiments disclosed in this application and should not be construed as limiting the scope of this application.
[0024] Figure 1 This figure shows the comparison results of the effects of different concentrations of galangal essential oil on the cell viability of HFDPCs in Example 1 of this application.
[0025] Figure 2 This is one of the diagrams showing the regulatory effect of Kaempferia galanga essential oil on germinative cytokines in HFDPCs cells in Example 2 of this application.
[0026] Figure 3 Figure 2 shows the regulatory effect of kaempferia galanga essential oil on germinal-related cytokines in HFDPCs cells in Example 2 of this application.
[0027] Figure 4 This image shows the comparison of hair growth areas on a mouse natural hair growth model after applying galangal essential oil to the mouse on day 14 in Example 3 of this application.
[0028] Figure 5 This is a comparison of the length of new hair after applying Kaempferia galanga essential oil to a mouse natural hair growth model for 21 days, as shown in Example 3 of this application.
[0029] Figure 6 This image shows the comparison of hair growth areas on a mouse model of androgenetic alopecia after applying galangal essential oil to the mouse model in Example 4 of this application on day 14.
[0030] Figure 7 This is a comparison of the length of new hair growth in a mouse model of androgenetic alopecia after 21 days of application of Kaempferia galanga essential oil in Example 4 of this application. Detailed Implementation
[0031] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this application. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.
[0032] This application provides a pure natural galangal essential oil with excellent hair growth and hair loss prevention effects. Galangal essential oil can promote the proliferation of human dermal papilla cells (HFDPCs), upregulate the secretion of VEGF (vascular endothelial growth factor) by these cells, downregulate the inhibition factor TGF-β1 (transforming growth factor beta), and improve the hair growth cycle. It can be used for early prevention or treatment of hair loss, overcoming the shortcomings of existing chemical drugs used for hair growth and hair loss prevention that are not natural and safe enough.
[0033] This application provides an example of the use of galangal essential oil in the preparation of skin application compositions.
[0034] In one embodiment, the skin application composition is a hair growth and hair loss prevention drug.
[0035] In one embodiment, the skin application composition is used to promote hair growth.
[0036] In one embodiment, promoting hair growth includes promoting the proliferation of human dermal dermal papilla cells, and / or regulating the levels of hair growth-related cell growth factors, and / or improving the hair growth cycle.
[0037] In one embodiment, the preparation method of the galangal essential oil includes steam distillation or supercritical carbon dioxide extraction.
[0038] In one embodiment, the total content of cinnamic acid esters, olefin compounds, borneol, and sitosterol in the galangal essential oil accounts for more than 90% of the total mass of the galangal essential oil. The hair growth and anti-hair loss effects of galangal essential oil are mainly due to the synergistic effect of the above-mentioned multiple components.
[0039] In one embodiment, the skin application composition is applied to a pharmaceutical preparation or a daily chemical product.
[0040] In one embodiment, the pharmaceutical preparation includes patches, tinctures, creams, ointments, gels, and sprays.
[0041] In one embodiment, the daily chemical products include shampoo, conditioner, anti-hair loss treatment liquid, hair oil, hair care essence, hair oil, hair lotion, scalp and hair nourishing products, hair essence, scalp cream, hair gel, hair spray, hair mask, and eyebrow care liquid.
[0042] It should be understood that in addition to galangal essential oil, daily chemical products also require common auxiliary ingredients such as emulsifiers, thickeners, surfactants, humectants, alcohols, water-soluble polymers, gelling agents, stabilizers, vitamins, inorganic salts, and fragrances. These auxiliary ingredients are all commonly used raw materials in existing technologies and will not be elaborated further.
[0043] In one embodiment, the galangal essential oil is added to the pharmaceutical preparation or daily chemical product at a mass percentage of 0.1%-20%. By setting this proportion of galangal essential oil, the pharmaceutical preparation or daily chemical product can contain an effective amount of galangal essential oil, thereby achieving the effects of hair growth and hair loss prevention.
[0044] Example 1: Preparation of Kaempferia galanga essential oil by supercritical extraction
[0045] The galangal herb was pulverized, and an appropriate amount was placed in a supercritical carbon dioxide extraction vessel. The extraction vessel temperature was 40–60℃, and the extraction vessel pressure was 15–35 MPa. The separation vessel temperature was 20–40℃, and the pressure was 4–6 MPa. The condensate temperature was 5℃, the carbon dioxide flow rate was 20–40 kg / h, and the extraction time was 1–3 h. Galangal essential oil was obtained from the separation vessel, with a yield of 5–8%.
[0046] Example 2: Preparation of Kaempferia galanga essential oil by steam distillation
[0047] Fresh or dried galangal root powder was placed in a round-bottom flask, and 6–10 times its volume of deionized water was added. The mixture was then heated under reflux using a volatile oil extraction apparatus for 4–6 hours. After extraction, the mixture was allowed to stand and anhydrous sodium sulfate was added to remove water, yielding a pale yellow galangal essential oil with a yield of 0.5–1%.
[0048] Kaempferia galanga essential oil can be extracted from either Example 1 or Example 2 above. The sample obtained from either preparation can be diluted to an appropriate concentration with chromatographic grade ethyl acetate, and the active ingredients can be qualitatively and quantitatively analyzed using gas chromatography-mass spectrometry (GC-MS).
[0049] The chromatographic column was a DB-5MS capillary column (30m × 0.25mm × 0.25μm); the ion source temperature was 250℃; the mass range was m / z 30~500; the injection volume was 1μL; the temperature program was: initial temperature 60℃, hold for 5 min, increase to 180℃ at a rate of 15℃ / min, then increase to 250℃ at a rate of 5℃ / min, hold for 5 min; the carrier gas was high-purity helium, injected in a split with a split ratio of 20:1; the carrier gas flow rate was 1mL / min; the standard library was the NIST spectral library, and the relative content was determined by area normalization. The quantitative results are shown in Table 1.
[0050] Table 1. Main components and relative content of galangal essential oil
[0051] Element Molecular formula Relative content (%) Ethyl p-methoxycinnamate <![CDATA[C 12 H 14 O3]]> 49.34 pentadecane <![CDATA[C 15 H 32 ]]> 18.79 Ethyl trans-cinnamate <![CDATA[C 11 H 12 O2]]> 14.55 Hexadecadiene <![CDATA[C 16 H 28 O]]> 4.34 Cyperene <![CDATA[C 15 H 24 ]]> 1.03 heptadecane <![CDATA[C 17 H 36 ]]> 0.99 Juniperene <![CDATA[C 15 H 24 ]]> 0.85 3-carene <![CDATA[C 10 H 16 ]]> 0.51 γ-sitosterol <![CDATA[C 29 H 50 O]]> 0.49 Borneol <![CDATA[C 10 H 18 O]]> 0.40
[0052] Example 3: Shampoo product containing galangal oil
[0053] A shampoo product containing galangal essential oil, the components and contents of which are shown in Table 2:
[0054] Table 2. Components and Content of Shampoo Containing Kaempferia Oil
[0055] Components Quality percentage (%) effect Kaempferia essential oil 2 Hair growth, hair care, and hair loss prevention Cocamidopropyl Betaine 3 surfactants Sodium lauryl ether sulfate 8 surfactants Citric acid 0.3 pH adjuster, anti-dandruff agent Phenoxyethanol 0.1 preservative Sodium benzoate 0.1 preservative Guar gum hydroxypropyltrimethylammonium chloride 1 Moisturizing fabric softener pearlescent agent 0.5 Deionized water Make up to 100 solvent
[0056] The preparation method includes the following steps: The prescribed amount of deionized water and surfactant are stirred and heated to 70°C until dissolved. Then, galangal oil is added, and the mixture is stirred at a constant temperature for 10 minutes. Next, a moisturizing and softening agent is added, and the mixture is stirred at a constant temperature for 20 minutes. After all raw materials are completely dissolved, heating is stopped and the mixture is cooled to below 45°C. Preservatives and pearlescent agents are added sequentially, and the pH is adjusted to 5.5-6.5 with citric acid. The mixture is then stirred and cooled to room temperature.
[0057] Example 4: Anti-hair loss care liquid containing galangal essential oil
[0058] The galangal essential oil obtained in Example 1 was selected and dissolved in a solvent system to prepare anti-hair loss care solutions with a mass percentage of 0%, 0.1%, 1%, and 10%, respectively. The solvent system consisted of 30%-40% anhydrous ethanol (for dissolution), 50% propylene glycol (for dissolution, preservation, and moisturizing), and 10%-20% deionized water.
[0059] Example 1: Proliferative effect of kaempferia galanga essential oil on human dermal papilla cells (HFDPCs)
[0060] Cell culture: Hair papilla cells (HFDPCs) were cultured in Dulbecco Modified Eagle Medium (DMEM) containing 10% (v / v) fetal bovine serum and 1% (v / v) antibiotics at 37°C and 5% CO2.
[0061] Sample preparation: The kaempferia essential oil obtained in Example 4 was dissolved in dimethyl sulfoxide (DMSO) and then diluted with culture medium to different concentrations of kaempferia essential oil (0.0002%-0.02%).
[0062] CCK-8 assay: Cells were sputtered at 5 × 10⁻⁶ 3Cells were seeded at a density of 1 / 2 well in 96-well plates and incubated for 24 h. Medium containing different concentrations of kaempferol oil was added to each well. Serum-free DMEM and 0.1% DMSO were used as blank and negative controls, respectively. After 24 h of treatment, 10% CCK-8 solution was added to each well, and the cells were incubated for another 1 h. Absorbance (OD) was measured at a detection wavelength of 450 nm. Cell viability (%) = (OD) / (Cell viability (%)) 样品 -OD 空白 ) / (OD 阴性对照 -OD 空白 The result is as follows: Figure 1 As shown, under co-incubation with different concentrations of kaempferia galanga essential oil, cell viability was significantly increased compared to the negative control group without essential oil, reaching a maximum of 129.59%. This demonstrates that kaempferia galanga essential oil can promote the proliferation of dermal papilla cells, thereby promoting hair growth.
[0063] Example 2: Regulation of germinal-related cytokines in HFDPCs cells by kaempferol essential oil
[0064] Cell culture: Same as in Example 1.
[0065] Sample preparation: Same as in Example 1, except that the concentration of galangal essential oil in the experimental group was 0.0002%-0.001%; in addition, minoxidil was prepared as a 0.0002% positive control group using the same sample preparation method.
[0066] Experimental Methods: The regulatory effects of VEGF (vascular endothelial growth factor) and TGF-β1 (transforming growth factor beta) on the culture supernatant obtained after treatment with ethyl p-methoxycinnamate were investigated using an ELISA kit (Xinbosheng, catalog numbers EHC108.96, EHC107b.96). The procedure was performed according to the kit instructions. Results are as follows: Figure 2 and Figure 3 As shown.
[0067] The results showed that co-incubation with kaempferol essential oil increased the amount of VEGF produced by human dermal papilla cells. VEGF plays a role in hair follicle growth and is a key indicator for promoting hair growth. On the other hand, minoxidil, as a positive control, significantly increased the amount of VEGF produced by human hair dermal papilla cells compared to the blank control. In addition, both the positive control and the experimental groups with different concentrations of kaempferol essential oil significantly reduced the production of TGF-β1. This factor is produced by androgens after being converted by reductase and binding to androgen receptors, regulating the apoptosis process of dermal papilla cells, leading to hair follicle atrophy and eventually hair loss.
[0068] Therefore, the results show that when galangal essential oil is applied to the scalp, it can increase the production of hair growth-related cytokines and resist the production of inhibitory factors, thereby achieving the effects of hair growth and hair strengthening.
[0069] Example 3: Hair growth effect of kaempferia galanga essential oil applied topically in a mouse model of natural hair growth.
[0070] Sample preparation: The dosage used in animal experiments needs to be scaled up at the cellular level. The kaempferia galanga essential oil prepared in Example 1 was dissolved in an ethanol solution to prepare 5% and 1% (w / w) kaempferia galanga essential oil samples for later use. Minoxidil, an internationally recognized treatment for hair loss, was dissolved in an ethanol solution as a positive control.
[0071] Experimental Methods: The effect of kaempferia galanga essential oil on hair follicle growth in mice was tested by applying it to the skin after hair removal on the back. C57BL / 6 mice were divided into four groups: experimental groups (1% kaempferia galanga essential oil group and 5% kaempferia galanga essential oil group), positive control group, and blank control group, with three mice in each group. After shaving the backs of the mice, 100 μL of kaempferia galanga essential oil sample was applied to the backs of the experimental group mice for 14 consecutive days (1% kaempferia galanga essential oil for experimental group 1, and 5% kaempferia galanga essential oil for experimental group 2). The positive control group was treated with 5% minoxidil, and the blank control group was treated with a blank solvent. After 14 days of application, the percentage of new hair growth was calculated based on the hair growth. Hair growth area (%) = area of newly grown hair on the back / area of hair removal * 100%.
[0072] Continue applying the medication until day 21. Carefully pluck about 10 hairs from 6 different areas on the mouse's back using tweezers, measure the length with calipers, and record the longest value.
[0073] Results of hair growth areas as follows Figure 4 As shown, the area of hair regeneration in each group of mice was calculated on day 14 after application. The color of the skin on the back of C57BL / 6 mice is closely related to the hair growth cycle. After hair loss, the skin changes from pink in the resting phase to gray or black, indicating that hair growth has re-entered the anagen phase. The results showed that after 14 days of application, new hair grew on the backs of mice in the galangal oil treatment group, and the growth area was significantly larger than that in the blank control group. The high-concentration galangal oil treatment group performed better than the positive control group. This indicates that galangal oil has a certain tendency to promote hair regeneration in mice.
[0074] Results of new hair growth length as follows Figure 5 As shown, after 21 days of application, hair regeneration was basically completed in all groups of mice. Both the positive control group and the experimental groups with different concentrations of kaempferia galanga oil showed a better hair growth trend compared to the blank control group.
[0075] Example 4: The anti-hair loss effect of kaempferia galanga essential oil on a mouse model of androgenetic alopecia.
[0076] Sample preparation: Dissolve the galangal essential oil and the positive control minoxidil from Example 1 in ethanol solution to prepare 5% concentration samples for later use. Dissolve testosterone propionate in corn oil for injection.
[0077] Experimental Methods: The antagonistic effect of kaempferol essential oil on androgen-induced hair growth inhibition was tested by applying it to the skin after hair removal on the back. C57BL / 6 mice were divided into four groups: blank control group, model group, positive control group, and experimental group, with three mice in each group. Except for the blank group, the other three groups were injected subcutaneously with 5 mg / kg of testosterone propionate daily to induce hair loss. Thirty minutes after injection, the blank control group and model group were treated with the blank solvent, the positive control group was treated with 5% minoxidil, and the experimental group was treated with 5% kaempferol essential oil, 100 μL each. The percentage of hair regeneration area was observed and calculated on day 14, using the formula shown in Example 3.
[0078] Continue applying the medication until day 21. Carefully pluck about 10 hairs from 6 different areas on the mouse's back using tweezers, measure the length with calipers, and record the longest value.
[0079] The calculation results of the hair regeneration area are as follows Figure 6 As shown, Figure 6 On day 14 after application, the ratio of hair regeneration area on the back of each group of mice was calculated. The results showed that after 14 days of continuous androgen injection, hair growth in the model group was inhibited due to the effect of hormones, and the area of hair regeneration was significantly reduced compared with the blank control group, proving that the model was successful. The effect of kaempferia galanga essential oil administration in the experimental group was similar to that in the positive control group. Both groups could antagonize the hair growth inhibition caused by androgens. Compared with the model group, the area of regeneration area increased significantly. It can be seen that kaempferia galanga essential oil can antagonize the process of androgenic alopecia to a certain extent and promote hair growth in mice in vivo.
[0080] Results of new hair growth length as follows Figure 7 As shown, the length of newly grown hair in the model group mice was shorter than that in the blank control group, while the positive control group and the galangal oil experimental group showed a significant increase in hair length.
[0081] Example 5: Survey of User Experiences with Kaempferia galanga essential oil shampoo
[0082] Volunteers aged 20-50 were divided into two groups to conduct user experience tests on the shampoo containing galangal essential oil prepared in Example 3 or the comparative example (formula as in Example 3, without galangal essential oil). The results of the questionnaire survey on user experience are shown in Table 3.
[0083] Table 3. User reviews of the galangal essential oil shampoo.
[0084] Comfort of use Cleanliness Hair quality improvement Reduce hair loss Example 3 ++ ++ +++ +++ Comparative Example ++ ++ ++ -
[0085] Note: In the survey results, - indicates no findings, + indicates basic satisfaction, ++ indicates relatively satisfactory, and +++ indicates very satisfactory.
[0086] The above-mentioned effect test results show that the present invention provides a shampoo containing galangal essential oil, which, compared with the control ratio, can improve hair quality, strengthen hair roots, and prevent hair loss.
[0087] Example 6: Survey of User Experiences with Kaempferia galanga Essential Oil Anti-Hair Loss Care Solution
[0088] Volunteers aged 20-50 years were selected for a randomized, blinded trial of the anti-hair loss treatment solution containing different concentrations of galangal essential oil prepared in Example 4. Three volunteers were assigned to each group, and the solution was used for one month. After shampooing, volunteers applied the solution to their scalp twice a day, 5 ml each time. The results of a questionnaire survey regarding the effectiveness of the treatment are shown in Table 4.
[0089] Table 4. Evaluation of the effectiveness of Kaempferia galanga essential oil anti-hair loss care solution
[0090]
[0091]
[0092] Note: In the survey results, - indicates no findings, + indicates basic satisfaction, ++ indicates relatively satisfactory, and +++ indicates very satisfactory.
[0093] The above-mentioned efficacy tests show that the present invention provides a hair loss prevention and care liquid containing galangal essential oil. This product can improve scalp health, prevent hair loss and nourish hair, and has a good user experience.
[0094] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.
[0095] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "a plurality of" means two or more, unless otherwise explicitly specified.
[0096] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this application, and these should all be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. The use of galangal essential oil as the sole active ingredient in the preparation of skin-applied compositions for promoting hair growth or preventing hair loss, characterized in that, The preparation methods of the galangal essential oil include steam distillation or supercritical carbon dioxide extraction. The supercritical carbon dioxide extraction method is as follows: the galangal herb is pulverized and an appropriate amount is placed in a supercritical carbon dioxide extraction vessel. The temperature of the extraction vessel is 40~60℃, the pressure of the extraction vessel is 15~35MPa, the temperature of the separation vessel is 20~40℃, the pressure is 4~6MPa, the temperature of the condensate is 5℃, the carbon dioxide flow rate is 20~40kg / h, and the extraction time is 1~3h. The steam distillation method is as follows: Take fresh or dried Kaempferia galanga in a round-bottom flask, add 6 to 10 times the volume of deionized water, and use a volatile oil extraction device to heat and reflux for 4 to 6 hours; after extraction, let it stand and add anhydrous sodium sulfate to remove water; The total content of cinnamic acid esters, olefin compounds, borneol and sitosterol in the kaempferol essential oil accounts for more than 90% of the total mass of the kaempferol essential oil. The skin-applied composition is a hair growth and hair loss prevention drug.
2. The use according to claim 1, characterized in that, The promotion of hair growth refers to promoting the proliferation of human dermal dermal papilla cells and / or regulating the level of hair growth-related cell growth factors and / or improving the hair growth cycle.
3. The use according to claim 1 or 2, characterized in that, The skin application composition is used in pharmaceutical preparations or daily chemical products.
4. The use according to claim 3, characterized in that, The pharmaceutical preparations include patches, tinctures, creams, ointments, gels, and sprays.
5. The use according to claim 3, characterized in that, The daily chemical products mentioned include shampoo, conditioner, anti-hair loss treatment liquid, hair oil, hair care essence, hair oil, hair lotion, scalp and hair nourishing products, hair essence, scalp cream, hair gel, hair spray, hair mask, and eyebrow care liquid.
6. The use according to claim 3, characterized in that, The galangal essential oil is added at a mass percentage of 0.1%-20% in pharmaceutical preparations or daily chemical products.