A functional polypeptide-polypeptide nanogold composite material modified anti-fouling glassy carbon electrode for detecting zeaxanthin

Abstract

The application belongs to the technical field of food detection, and relates to a functional polypeptide-polypeptide gold nanoparticle composite material modified anti-pollution glassy carbon electrode for detecting zeamacorin. The electrode is prepared by the following steps: adopting a Gaussian 09 series program, optimizing the structure of the functional polypeptide at B3LYP / 6-31G(d,p) by density functional theory and using a polarizable continuum model (IEF-PCM) of integral equation to obtain the optimal functional polypeptide sequence. The functional polypeptide is dropped on the surface of the glassy carbon electrode, dried, and then PA-AuNPs-2 solution is dropped, and then antibody solution is added and cultured. Then, the electrode is incubated with BSA solution to obtain the functional polypeptide-polypeptide gold nanoparticle composite material anti-pollution glassy carbon electrode. The electrode can be used for detecting zeamacorin and its derivatives, and can improve the anti-pollution ability and detection signal strength of the electrode, and increase the accuracy and reliability of the detection of zeamacorin and its derivatives in actual samples.

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