一种含有桃叶珊瑚苷的猪精液存储液及其制备方法

By adding aucubin to the diluent for boar semen, the problem of ROS accumulation during room temperature storage of boar semen was solved, improving sperm motility and storage time while reducing costs.

CN117502428BActive Publication Date: 2026-07-17NORTHWEST A & F UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Filing Date
2023-10-26
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies for preserving bovine semen at room temperature, as the preservation time increases, the accumulation of reactive oxygen species (ROS) in the semen leads to oxidative stress in the sperm, affecting sperm motility and fertilization capacity, and is also costly.

Method used

A porcine semen storage solution was prepared using a diluent containing 1-100 μg/ml aucubin, with a formulation including glucose, sodium citrate, EDTA, sodium bicarbonate, potassium chloride, and double-distilled water. The solution's antioxidant properties extended the semen's storage time.

Benefits of technology

It significantly improves sperm motility and plasma membrane integrity, reduces MDA content, enhances total antioxidant capacity, extends semen storage by more than one week, and reduces storage costs.

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Abstract

本发明提供了一种含有桃叶珊瑚苷的猪精液存储液及其制备方法,通过在猪精液常温保存稀释液原液中加入桃叶珊瑚苷,并保持其浓度在12.5‑25μg / m l。在桃叶珊瑚苷的抗氧化性能作用下,可有效提高精子活率和质膜完整性,提高总抗氧化能力(T‑AOC)和超氧化物歧化酶(SOD)浓度,同时降低保存环境中的丙二醛(MDA)和活性氧(ROS)浓度,使猪精液的保存时间在常温下可以达到7天以上,精子活力在80%以上,精子活率在0.80以上。同时因为桃叶珊瑚苷普遍存在于多种传统中药中,可供提取的植物大多廉价易得,降低了猪精液保存成本,对进一步推广人工授精技术的应用,促进我国养猪业健康持续发展具有重要意义。
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