A hemodialysis membrane and a method for producing the same

Abstract

The application belongs to the technical field of biological medical materials, and discloses a hemodialysis membrane and a preparation method thereof. The preparation method of the hemodialysis membrane comprises the following steps: mixing polyether sulfone, nitric acid and sulfuric acid to obtain nitration polyether sulfone through a reaction; mixing the nitration polyether sulfone, stannous chloride, hydrochloric acid and ethanol to obtain aminated polyether sulfone through a reaction; mixing the aminated polyether sulfone with a solvent to obtain an aminated polyether sulfone membrane through an immersion precipitation phase inversion method; immersing the aminated polyether sulfone membrane in a mixed solution of white hulless bran alcohol to obtain a modified aminated polyether sulfone membrane; and immersing the modified aminated polyether sulfone membrane in a mixed solution of polyacryloyl morpholine to obtain the hemodialysis membrane. The hemodialysis membrane in the application can effectively intervene in oxidative stress under the premise of good biocompatibility, and the preparation method is simple.

Description

Technical Field

[0001] This invention relates to the field of biomedical materials technology, and in particular to a hemodialysis membrane and its preparation method. Background Technology

[0002] Hemodialysis is currently one of the effective treatments for chronic kidney disease, relying on the diffusion and ultrafiltration between blood and dialysate. Driven by the concentration gradient, metabolic waste and toxins in the blood diffuse through the semipermeable membrane to the dialysate side, thereby removing toxins from the patient's body. The hemodialysis membrane is a crucial component of the hemodialysis process.

[0003] Hemodialysis membranes are divided into two types: cellulose-based membranes and polymeric synthetic membranes. Cellulose-based membranes mainly include regenerated cellulose and modified cellulose membranes, while polymeric synthetic membranes mainly include polysulfone, polyethersulfone, and polyacrylonitrile. Because polymeric materials are generally hydrophobic, their biocompatibility and blood compatibility are poor. When in contact with blood, they can cause an oxygen burst in leukocytes, leading to increased levels of reactive oxygen species and inflammatory factors in the blood. Furthermore, this oxygen burst may continue to increase after a dialysis session. Therefore, dialysis membranes need to have a certain degree of antioxidant capacity to effectively reduce oxidative stress in patients during dialysis, thus mitigating the significant impact on their quality of life.

[0004] Therefore, developing a hemodialysis membrane that can inhibit oxidative stress is an urgent problem to be solved. Summary of the Invention

[0005] The purpose of this invention is to provide a hemodialysis membrane and its preparation method to solve the problem of oxidative stress in existing hemodialysis membranes.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for preparing a hemodialysis membrane, comprising the following steps: A first reaction is carried out by mixing polyethersulfone, nitric acid and sulfuric acid to obtain nitrated polyethersulfone. Then, a second reaction is carried out by mixing nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol to obtain aminated polyethersulfone. Ammoniated polyethersulfone is mixed with a solvent, dispersed, allowed to stand to degas, and then subjected to an immersion precipitation phase inversion method to obtain an ammoniated polyethersulfone membrane. Resveratrol, tris(hydroxymethyl)aminomethane, metal salt and water are mixed to obtain a resveratrol mixed solution; Polyacrylamide morpholine, tris(hydroxymethyl)aminomethane and water were mixed to obtain a polyacrylamide morpholine mixed solution; The modified polyethersulfone membrane was immersed in a resveratrol mixed solution to obtain the modified polyethersulfone membrane. The modified ammoniated polyethersulfone membrane was immersed in a mixed solution of polyacrylmorpholine to obtain a hemodialysis membrane.

[0007] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the ratio of polyethersulfone, nitric acid and sulfuric acid is 8~15g: 20~40mL: 30~50mL; The ratio of nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol is 8~15g:15~35g:15~35mL:40~60mL.

[0008] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the temperature of the first reaction is 55~70℃, and the time of the first reaction is 5~7h; The temperature of the second reaction is 55~70℃, and the reaction time is 4~6h.

[0009] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the ratio of the ammoniated polyethersulfone to the solvent is 1g:5~6mL; The solvent is N,N-dimethylformamide, N,N-dimethylacetamide, or N-methylpyrrolidone.

[0010] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the ratio of resveratrol, tris(hydroxymethyl)aminomethane, metal salt and water is 1~3g: 1~3g: 8~15g: 0.5~1L; The ratio of polyacrylamide morpholine, tris(hydroxymethyl)aminomethane, and water is 1-5 g: 1-3 g: 0.5-1 L.

[0011] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the metal salt is sodium chloride, magnesium chloride, or ferric chloride.

[0012] Preferably, in the above-mentioned method for preparing a hemodialysis membrane, the soaking temperature is independently 20~30℃, and the soaking time is independently 15~20min.

[0013] The present invention also provides a method for preparing a hemodialysis membrane and a hemodialysis membrane obtained therefrom.

[0014] As can be seen from the above technical solution, compared with the prior art, the present invention has the following beneficial effects: The resveratrol used in this invention is a plant-based natural polyphenol antioxidant. Its phenolic hydroxyl groups have the ability to release hydrogen atoms, or ionize to produce H+. +After releasing electrons, the phenolic hydroxyl group is eventually oxidized to quinone, thereby scavenging free radical ions; polyacrylamide morpholine has good hydrophilicity and biocompatibility. In this invention, polyethersulfone is structurally modified to obtain aminated polyethersulfone, which imparts excellent hydrophilicity. After membrane formation, resveratrol is introduced without affecting the main structure, and then the hydrogen bonding between resveratrol and polyacrylamide morpholine is used to achieve the self-assembly of resveratrol and polyacrylamide morpholine to obtain a hemodialysis membrane. This allows the hemodialysis membrane to effectively intervene in oxidative stress while having good biocompatibility; at the same time, it has excellent dialysis performance. The preparation method of the hemodialysis membrane in this invention is simple and can be widely applied. Detailed Implementation

[0015] This invention provides a method for preparing a hemodialysis membrane, comprising the following steps: A first reaction is carried out by mixing polyethersulfone, nitric acid and sulfuric acid to obtain nitrated polyethersulfone. Then, a second reaction is carried out by mixing nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol to obtain aminated polyethersulfone. Ammoniated polyethersulfone is mixed with a solvent, dispersed, allowed to stand to degas, and then subjected to an immersion precipitation phase inversion method to obtain an ammoniated polyethersulfone membrane. Resveratrol, tris(hydroxymethyl)aminomethane and water were mixed to obtain a resveratrol mixed solution; A mixed solution of polyacrylmorpholine, tris(hydroxymethyl)aminomethane, a metal salt, and water was obtained. The modified polyethersulfone membrane was immersed in a resveratrol mixed solution to obtain the modified polyethersulfone membrane. The modified ammoniated polyethersulfone membrane was immersed in a mixed solution of polyacrylmorpholine to obtain a hemodialysis membrane.

[0016] In this invention, the preferred ratio of polyethersulfone, nitric acid and sulfuric acid is 8-15g: 20-40mL: 30-50mL, more preferably 9-13g: 25-38mL: 35-45mL, and even more preferably 10-12g: 28-35mL: 38-42mL.

[0017] In this invention, the nitric acid is preferably pure nitric acid, and the sulfuric acid is preferably pure sulfuric acid.

[0018] In this invention, the specific process of mixing polyethersulfone, nitric acid and sulfuric acid is as follows: nitric acid and sulfuric acid are mixed, cooled and then added dropwise to polyethersulfone.

[0019] In this invention, the temperature of the first reaction is preferably 55~70℃, more preferably 58~68℃, and even more preferably 60~65℃; the time of the first reaction is preferably 5~7h, more preferably 5.25~6.5h, and even more preferably 5.5~6h.

[0020] In this invention, after the first reaction is completed, the process further includes washing the obtained product sequentially with distilled water and ethanol, and then drying it.

[0021] In this invention, the preferred ratio of nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol is 8-15g:15-35g:15-35mL:40-60mL, more preferably 9-13g:18-30g:18-30mL:42-58mL, and even more preferably 10-12g:20-25g:20-25mL:45-55mL.

[0022] In this invention, the concentration of the hydrochloric acid is preferably 35-38 wt%, more preferably 35-37 wt%, and even more preferably 37 wt%.

[0023] In this invention, the ethanol is preferably anhydrous ethanol.

[0024] In this invention, the specific process of mixing the nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol is as follows: first, stannous chloride is mixed and dissolved with hydrochloric acid, then ethanol is added, and after mixing and stirring, nitrated polyethersulfone is added.

[0025] In this invention, the temperature of the second reaction is preferably 55~70℃, more preferably 58~68℃, and even more preferably 60~65℃; the time of the second reaction is preferably 4~6h, more preferably 4.5~5.5h, and even more preferably 5h.

[0026] In this invention, after the second reaction is completed, the process further includes washing the obtained product sequentially with distilled water and ethanol, and then drying it.

[0027] In this invention, the ratio of the ammoniated polyethersulfone to the solvent is preferably 1g:5~6mL, more preferably 1g:5.2~5.8mL, and even more preferably 1g:5.4~5.6mL.

[0028] In this invention, the solvent is preferably N,N-dimethylformamide, N,N-dimethylacetamide or N-methylpyrrolidone, more preferably N,N-dimethylformamide or N,N-dimethylacetamide; more preferably N,N-dimethylformamide.

[0029] In this invention, the dispersion temperature is preferably 70~85℃, more preferably 75~82℃, and even more preferably 78~80℃; the dispersion time is preferably 15~20min, more preferably 16~19min, and even more preferably 17~18min.

[0030] In this invention, the thickness of the ammoniated polyethersulfone film is preferably 10-15µm, more preferably 11-14µm, and even more preferably 12-13µm.

[0031] In this invention, the preferred ratio of resveratrol, tris(hydroxymethyl)aminomethane, metal salt and water is 1~3g:1~3g:8~15g:0.5~1L, more preferably 1.2~2.5g:1.2~2.5g:10~14g:0.6~0.9L, and even more preferably 1.5~2g:1.5~2g:11~13g:0.7~0.8L.

[0032] In this invention, the preferred ratio of polyacrylamide morpholine, tris(hydroxymethyl)aminomethane, and water is 1-5 g: 1-3 g: 0.5-1 L, more preferably 2-4 g: 1.2-2.5 g: 0.6-0.9 L, and even more preferably 2.5-3.5 g: 1.5-2 g: 0.7-0.8 L.

[0033] In this invention, the metal salt is preferably sodium chloride, magnesium chloride or ferric chloride, more preferably sodium chloride or magnesium chloride, and even more preferably sodium chloride.

[0034] In this invention, the soaking temperature is preferably 20-30°C, more preferably 22-28°C, and even more preferably 25-26°C; the soaking time is preferably 15-20 min, more preferably 16-19 min, and even more preferably 17-18 min.

[0035] The present invention also provides a method for preparing a hemodialysis membrane and a hemodialysis membrane obtained therefrom.

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Example 1

[0038] A method for preparing a hemodialysis membrane includes the following steps: (1) Mix 40 mL of pure sulfuric acid with 30 mL of pure nitric acid, cool and then add 10 g of polyether sulfone. Stir at 60 °C for 6 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain nitrated polyether sulfone. Mix 25 g of stannous chloride with 30 mL of 37 wt% hydrochloric acid to dissolve, then add 50 mL of anhydrous ethanol and stir at 60 °C for 15 min. After stirring, add 10 g of nitrated polyether sulfone and continue to react at 60 °C for 4 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain aminated polyether sulfone. (2) 10g of ammoniated polyethersulfone and 50mL of N,N-dimethylformamide were stirred and mixed at 80℃ for 15min, and then allowed to stand and degas in a drying oven at 60℃. The ammoniated polyethersulfone film with a thickness of 12µm was obtained by immersion precipitation phase inversion method. (3) Mix and disperse 2g resveratrol, 1g tris(hydroxymethyl)aminomethane, 15g sodium chloride and 1L water for 10min to obtain a resveratrol mixed solution; mix and disperse 3g polyacrylamide, 1g tris(hydroxymethyl)aminomethane and 1L water for 10min to obtain a polyacrylamide mixed solution. (4) The aminated polyethersulfone membrane was immersed in a resveratrol mixed solution at 25°C for 15 min to obtain the modified aminated polyethersulfone membrane. (5) The modified ammoniated polyethersulfone membrane was immersed in a polyacrylamide morpholine mixed solution at 25°C for 15 min to obtain a hemodialysis membrane.

[0039] The ultrafiltration rate of the hemodialysis membrane prepared above was tested to be 45.5 mL / (m). 2 The urea clearance rate was 91.4%, the urinary albumin clearance rate was 79.7%, and the β2-microglobulin clearance rate was 86.2%.

[0040] Example 2

[0041] A method for preparing a hemodialysis membrane includes the following steps: (1) Mix 40 mL of pure sulfuric acid with 30 mL of pure nitric acid, cool and then add 15 g of polyether sulfone. Stir at 60 °C for 6 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain nitrated polyether sulfone. Mix 25 g of stannous chloride with 35 mL of 37 wt% hydrochloric acid to dissolve, then add 50 mL of anhydrous ethanol and stir at 60 °C for 15 min. After stirring, add 15 g of nitrated polyether sulfone and continue to react at 60 °C for 5 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain aminated polyether sulfone. (2) 15g of ammoniated polyethersulfone and 75mL of N,N-dimethylformamide were stirred and mixed at 80℃ for 15min, and then allowed to stand and degas in a drying oven at 60℃. The ammoniated polyethersulfone film with a thickness of 12µm was obtained by immersion precipitation phase inversion method. (3) Mix and disperse 3g resveratrol, 2g tris(hydroxymethyl)aminomethane, 15g sodium chloride and 1L water for 15min to obtain a resveratrol mixed solution; mix and disperse 4g polyacrylamide, 2g tris(hydroxymethyl)aminomethane and 1L water for 15min to obtain a polyacrylamide mixed solution. (4) The aminated polyethersulfone membrane was immersed in a resveratrol mixed solution at 25°C for 20 min to obtain the modified aminated polyethersulfone membrane; (5) The modified ammoniated polyethersulfone membrane was immersed in a polyacrylamide morpholine mixed solution at 25°C for 20 min to obtain a hemodialysis membrane.

[0042] The ultrafiltration rate of the hemodialysis membrane prepared above was tested to be 47.2 mL / (m). 2 The urea clearance rate was 95.1%, the urinary albumin clearance rate was 81.5%, and the β2-microglobulin clearance rate was 87.3%.

[0043] Example 3

[0044] A method for preparing a hemodialysis membrane includes the following steps: (1) Mix 40 mL of pure sulfuric acid with 30 mL of pure nitric acid, cool and then add 12 g of polyether sulfone. Stir at 65 °C for 5 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain nitrated polyether sulfone. Mix 20 g of stannous chloride with 30 mL of 37 wt% hydrochloric acid to dissolve, then add 50 mL of anhydrous ethanol and stir at 65 °C for 15 min. After stirring, add 12 g of nitrated polyether sulfone and continue to react at 65 °C for 4 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain aminated polyether sulfone. (2) 12g of ammoniated polyethersulfone and 65mL of N,N-dimethylformamide were stirred and mixed at 80℃ for 15min, and then allowed to stand and degas in a drying oven at 60℃. The ammoniated polyethersulfone film with a thickness of 12µm was obtained by immersion precipitation phase inversion method. (3) Mix and disperse 2g resveratrol, 1g tris(hydroxymethyl)aminomethane, 15g sodium chloride and 1L water for 15min to obtain a resveratrol mixed solution; mix and disperse 3g polyacrylamide morpholine, 1g tris(hydroxymethyl)aminomethane and 1L water for 15min to obtain a polyacrylamide morpholine mixed solution. (4) The aminated polyethersulfone membrane was immersed in a resveratrol mixed solution at 25°C for 20 min to obtain the modified aminated polyethersulfone membrane; (5) The modified ammoniated polyethersulfone membrane was immersed in a polyacrylamide morpholine mixed solution at 25°C for 15 min to obtain a hemodialysis membrane.

[0045] The ultrafiltration rate of the hemodialysis membrane prepared above was tested to be 44.3 mL / (m). 2 The urea clearance rate was 92.8%, the urinary albumin clearance rate was 79.2%, and the β2-microglobulin clearance rate was 84.8%.

[0046] Example 4

[0047] A method for preparing a hemodialysis membrane includes the following steps: (1) Mix 40 mL of pure sulfuric acid with 30 mL of pure nitric acid, cool and then add 8 g of polyether sulfone. Stir at 65 °C for 5 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain nitrated polyether sulfone. Mix 20 g of stannous chloride with 30 mL of 37 wt% hydrochloric acid to dissolve, then add 50 mL of anhydrous ethanol and stir at 65 °C for 15 min. After stirring, add 8 g of nitrated polyether sulfone and continue to react at 65 °C for 4 h. After the reaction, wash the product with distilled water and ethanol in sequence, and then dry to obtain aminated polyether sulfone. (2) 8g of ammoniated polyethersulfone and 45mL of N,N-dimethylformamide were stirred and mixed at 80℃ for 15min, and then allowed to stand and degas in a drying oven at 60℃. The ammoniated polyethersulfone film with a thickness of 12µm was obtained by immersion precipitation phase inversion method. (3) Mix and disperse 2g resveratrol, 2g tris(hydroxymethyl)aminomethane, 15g sodium chloride and 1L water for 15min to obtain a resveratrol mixed solution; mix and disperse 3g polyacrylamide morpholine, 2g tris(hydroxymethyl)aminomethane and 1L water for 15min to obtain a polyacrylamide morpholine mixed solution. (4) The aminated polyethersulfone membrane was immersed in a resveratrol mixed solution at 25°C for 20 min to obtain the modified aminated polyethersulfone membrane; (5) The modified ammoniated polyethersulfone membrane was immersed in a polyacrylamide morpholine mixed solution at 25°C for 15 min to obtain a hemodialysis membrane.

[0048] The ultrafiltration rate of the hemodialysis membrane prepared above was tested to be 42.7 mL / (m). 2 The urea clearance rate was 91.0%, the urinary albumin clearance rate was 78.3%, and the β2-microglobulin clearance rate was 83.2%.

[0049] To determine the membrane's ability to scavenge free radicals and its antioxidant capacity under simulated blood stress, DPPH· and ABTS· free radicals were analyzed. + Free radical scavenging test: (1) Scavenging test of DPPH free radicals A 0.1 mM solution of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH·) was prepared using ethanol as a solvent. 1 cm 2 The hemodialysis membranes prepared in Examples 1-4 were added to 3 mL of the above solution and reacted at 37°C in the dark for 45 min. The absorbance of the solution at 517 nm was then measured.

[0050] Specific calculation formula: DPPH Scavenging Capacity = (A c -A s ) / A c ×(m / S); A c Absorbance of DPPH solution without membrane; A s To add 1cm 2 Absorbance of the DPPH· solution of the membrane; m is the mass of DPPH·, μg; S is the area of ​​the membrane, cm². 2 The calculation results are shown in Table 1.

[0051] (2) ABTS· + Cleanup test

[0052] First, add an equal volume of 2.45 mM potassium persulfate solution to 7 mM 2,2'-azido-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS· + Mix thoroughly and store in the dark for 12 hours to obtain ABTS· + Solution. Dissolve ABTS· in anhydrous ethanol. + The solution was diluted to an absorbance of 0.700 ± 0.025 (at 734 nm). 4 mL of ABTS was then used. + Solution, then add 1cm 2 The hemodialysis membranes prepared in Examples 1-4 were reacted at room temperature in the dark for 20 minutes, and the absorbance of the solution at 734 nm was measured.

[0053] Specific calculation formula: ABTS· + Clearance capability = (A c -A s ) / A c ×(m / S); A c ABTS without membrane + Solution absorbance; A s To add 1cm2 ABTS of membranes + Solution absorbance; m is ABTS· + The mass of the membrane is μg; S is the area of ​​the membrane, cm². 2 The calculation results are shown in Table 1.

[0054] Table 1. DPPH· free radicals and ABTS· free radicals in hemodialysis membranes in Examples 1-4 + Free radical scavenging ability

[0055] As shown in Table 1, the hemodialysis membrane prepared in this invention has a scavenging capacity of 140.4 µg / cm³ for DPPH free radicals. 2 For ABTS· + Its removal capacity can reach 269.5 µg / cm³. 2 This demonstrates that the hemodialysis membrane prepared by this invention represents a significant technological advancement in terms of its ability to resist oxidative stress.

[0056] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing a hemodialysis membrane, characterized in that, Includes the following steps: A first reaction is carried out by mixing polyethersulfone, nitric acid and sulfuric acid to obtain nitrated polyethersulfone. Then, a second reaction is carried out by mixing nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol to obtain aminated polyethersulfone. Ammoniated polyethersulfone is mixed with a solvent, dispersed, allowed to stand to degas, and then subjected to an immersion precipitation phase inversion method to obtain an ammoniated polyethersulfone membrane. Resveratrol, tris(hydroxymethyl)aminomethane, metal salt and water are mixed to obtain a resveratrol mixed solution; Polyacrylamide morpholine, tris(hydroxymethyl)aminomethane and water were mixed to obtain a polyacrylamide morpholine mixed solution; The modified polyethersulfone membrane was immersed in a resveratrol mixed solution to obtain the modified polyethersulfone membrane. The modified ammoniated polyethersulfone membrane was immersed in a mixed solution of polyacrylmorpholine to obtain a hemodialysis membrane.

2. The method for preparing the hemodialysis membrane according to claim 1, characterized in that, The ratio of polyethersulfone, nitric acid and sulfuric acid is 8~15g: 20~40mL: 30~50mL; The ratio of nitrated polyethersulfone, stannous chloride, hydrochloric acid and ethanol is 8~15g:15~35g:15~35mL:40~60mL.

3. The method for preparing the hemodialysis membrane according to claim 2, characterized in that, The temperature of the first reaction is 55~70℃, and the reaction time is 5~7h; The temperature of the second reaction is 55~70℃, and the reaction time is 4~6h.

4. The method for preparing the hemodialysis membrane according to claim 3, characterized in that, The ratio of the ammoniated polyethersulfone to the solvent is 1g:5~6mL; The solvent is N,N-dimethylformamide, N,N-dimethylacetamide, or N-methylpyrrolidone.

5. The method for preparing the hemodialysis membrane according to claim 4, characterized in that, The ratio of resveratrol, tris(hydroxymethyl)aminomethane, metal salt and water is 1~3g: 1~3g: 8~15g: 0.5~1L; The ratio of polyacrylamide morpholine, tris(hydroxymethyl)aminomethane, and water is 1-5 g: 1-3 g: 0.5-1 L.

6. The method for preparing the hemodialysis membrane according to claim 5, characterized in that, The metal salt is sodium chloride, magnesium chloride, or ferric chloride.

7. The method for preparing the hemodialysis membrane according to claim 6, characterized in that, The ammoniated polyethersulfone membrane is immersed in a resveratrol mixed solution at a temperature of 20-30°C for 15-20 minutes; the modified ammoniated polyethersulfone membrane is immersed in a polyacrylamide morpholine mixed solution at a temperature of 20-30°C for 15-20 minutes.

8. A hemodialysis membrane prepared by the method of any one of claims 1 to 7.

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