检测地龙多肽及多肽地龙蛋白片荧光传感器试剂及其制备及应用
By reacting the PFC-1 fluorescent sensor reagent with earthworm polypeptide NK-12 and utilizing the change in its fluorescence intensity, the problem of detecting earthworm polypeptide NK-12 has been solved, achieving a detection effect with high sensitivity and accuracy, which is suitable for the evaluation of earthworm drugs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
- Filing Date
- 2023-11-08
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of effective methods in the current technology for detecting the content of earthworm polypeptides, especially the content of earthworm polypeptide NK-12, affects the evaluation of the relevant effects of earthworm medicines.
PFC-1 was used as a fluorescent sensor reagent. It was reacted with earthworm polypeptide NK-12 in suspension form. The concentration of earthworm polypeptide NK-12 was detected by the change in fluorescence intensity. Combined with PBS buffer, specific pH value and reaction time, a "turn-off" fluorescent sensor was constructed.
A highly sensitive, accurate, and repeatable detection method for earthworm polypeptide NK-12 was achieved, with a detection limit of 1.44 ng/mL and a recovery rate ranging from 89.58% to 102.24%. This method is suitable for the detection of earthworm medicinal materials, processed products, extracts, and polypeptide earthworm protein tablets.
Smart Images
Figure CN117511536B_ABST