一种QuEChERS净化超高效液相色谱法检测药食两用物质中赭曲霉毒素B的方法

The QuEChERS purification ultra-high performance liquid chromatography method has solved the problem of detecting ochratoxin B in food and medicine substances, realizing simple, rapid and sensitive qualitative and quantitative analysis, and is suitable for the efficient detection of a variety of food and medicine substances.

CN117517548BActive Publication Date: 2026-07-17NANJING NORMAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Filing Date
2023-12-25
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies lack a suitable detection method for ochratoxin B in substances that are both food and medicine. Furthermore, traditional pretreatment methods are complex, costly, and unsuitable for large-scale sample testing, making it difficult to achieve effective extraction and quantitative analysis of OTB toxins.

Method used

The QuEChERS purification ultra-high performance liquid chromatography method is used, which includes sample pretreatment, QuEChERS salting out, and QuEChERS purification steps. Combined with ultra-high performance liquid chromatography-fluorescence detector, a variety of medicinal and edible substances are purified and detected by using a specific ratio of purifying agent.

Benefits of technology

It achieves highly sensitive detection of ochratoxin B in food and medicine substances, simplifies the pretreatment process, reduces costs, is suitable for large batches of samples, and has detection and quantitation limits lower than national standards, making it widely applicable.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一种QuEChERS净化超高效液相色谱法检测药食两用物质中赭曲霉毒素B的方法,包括如下步骤:药食两用物质粉碎均匀后,采用含甲酸‑乙腈混合溶液超声提取后离心;取离心后上清液,上清液加入QuEChERS盐析包进行盐析,涡旋后进行离心;取离心后上清液即乙腈层,加入QuEChERS净化包进行净化,涡旋后离心;取上清液吹干后,采用乙腈‑甲酸水溶液复溶,过有机滤膜,准备超高效液相色谱‑荧光检测器进样;以C18为色谱柱,采用超高效液相色谱结合荧光检测器对获得的样品进行定性与定量分析。本发明的方法操作简便、定性定量准确,可作为检测药食两用物质中OTB毒素的可靠方法。
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