Cleaning compositions and methods of making the same

Abstract

Provided is a cleaning composition which can inhibit the decrease in the enzymatic activity of a proteolytic enzyme or a lipolytic enzyme and is also excellent in use properties. A cleaning composition containing (A) a higher fatty acid and / or a salt thereof, (B) a proteolytic enzyme and / or a lipolytic enzyme, (C) a polyhydric alcohol, and (D) water, the content of the above (C) being 25 to 60 mass% relative to the total amount of the cleaning composition, the content of the above (D) being 5 to 30 mass% relative to the total amount of the cleaning composition, and as the above (C), glycerin and / or sorbitol are contained, the content of the above glycerin and / or sorbitol being 10 to 60 mass% relative to the total amount of the cleaning composition.

Description

Technical Field

[0001] This invention relates to cleaning compositions. More specifically, it relates to cleaning compositions and methods for manufacturing the same, capable of inhibiting the decrease in activity of proteolytic enzymes or lipidolytic enzymes and stably maintaining the activity of such enzymes. Background Technology

[0002] Previously, cleansing compositions containing enzymes were proposed for the purpose of removing skin dirt from the stratum corneum, sweat gland secretions, etc. For example, Patent Document 1 discloses a facial cleansing powder containing a protein-degrading enzyme derived from microorganisms.

[0003] Existing technical documents

[0004] Patent documents

[0005] Patent Document 1: Japanese Patent Application Publication No. 2009-256211 Summary of the Invention

[0006] The problem the invention aims to solve

[0007] Enzymes such as proteolytic enzymes experience a decrease or inactivation of their activity when stored in water for a certain period. To maintain enzyme activity in cleansing compositions, it is generally necessary to drastically reduce the water content within the composition. However, drastically reducing the water content leads to usability issues. For example, there is a tendency for the cleansing composition to have reduced affinity for water, resulting in decreased water solubility during use; and a tendency for reduced sprayability when filled into a tubular container, among other usability problems. On the other hand, to prevent enzyme inactivation, there are also product specifications that repackage powdered facial cleansers into single-use portions, but there is also a strong demand for products that can be easily used in tubular containers or similar containers.

[0008] Therefore, the present invention provides a cleaning composition that can inhibit the reduction of enzyme activity and has excellent usability.

[0009] Solution for solving the problem

[0010] In view of the above, the inventors have repeatedly conducted in-depth research and unexpectedly discovered that by mixing protein-degrading enzymes and lipid-degrading enzymes with higher fatty acids and / or their salts, specific polyols and water, and controlling the content of the polyols and water to a specific range, a cleaning composition that can inhibit the reduction of enzyme activity of protein-degrading enzymes or lipid-degrading enzymes and has excellent usability can be provided.

[0011] That is, the present invention provides the following [1] to

[10] .

[0012] [1] A cleaning composition comprising (A) higher fatty acids and / or their salts, (B) proteolytic enzymes and / or lipolytic enzymes, (C) polyols and (D) water, wherein the content of (C) is 25 to 60% by mass relative to the total amount of the cleaning composition, and the content of (D) is 5 to 30% by mass relative to the total amount of the cleaning composition, wherein (C) comprises glycerol and / or sorbitol, and the content of glycerol and / or sorbitol is 10 to 60% by mass relative to the total amount of the cleaning composition.

[0013] [2] The cleaning composition according to [1], wherein (A) is a higher fatty acid having 12 to 18 carbon atoms and / or its salt.

[0014] [3] The cleaning composition according to [1] or [2], wherein (B) is a protein-degrading enzyme and / or lipid-degrading enzyme in an unimmobilized state.

[0015] [4] The cleaning composition according to any one of [1] to [3], wherein (B) is an alkaline protein-degrading enzyme and / or an alkaline lipid-degrading enzyme.

[0016] [5] The cleaning composition according to any one of [1] to [4], wherein, as described above (C), it contains glycerin.

[0017] [6] The cleaning composition according to any one of [1] to [5], wherein the content of (D) above is 15 to 30% by mass relative to the total amount of the cleaning composition.

[0018] [7] The cleaning composition according to any one of [1] to [6] further contains (E) pH adjuster.

[0019] [8] The cleaning composition according to any one of [1] to [7], wherein the pH of the cleaning composition is 8.0 to 12.0.

[0020] [9] The cleaning composition according to any one of [1] to [8] is filled into a tubular container.

[0021] The method for manufacturing the cleaning composition described in any one of

[10] [1] to [8] includes the following steps: adding phase II containing (A) which is dissolved by heating at 60 to 80°C to phase I containing the above (C) and (D) heated to 60°C or higher, stirring, and then adding (B) at 55°C or lower after a cooling step.

[0022] The effects of the invention

[0023] According to the present invention, a cleaning composition that can inhibit the reduction of enzyme activity and has excellent usability can be provided. Detailed Implementation

[0024] The embodiments of the present invention will be described in detail below. However, the present invention is not limited to the following embodiments.

[0025] In this specification, the numerical range described using "X~Y" (where X and Y are arbitrary numbers) generally refers to "above X and below Y" unless otherwise specified. Regarding the numerical ranges described in stages in this specification, the upper or lower limit of a numerical range for a certain stage can be arbitrarily combined with the upper or lower limit of numerical ranges for other stages. Furthermore, the upper or lower limit of the numerical range described in this specification can also be replaced with the values ​​shown in the examples or formulation examples.

[0026] In addition, in this specification, "α and / or β" (α and β are any composition or component) means, unless otherwise specified, a combination of the three: "α only", "β only", or "both α and β".

[0027] <Cleaning Composition>

[0028] The cleaning composition described in the embodiments of the present invention is a cleaning composition containing (A) higher fatty acids and / or their salts, (B) proteolytic enzymes and / or lipolytic enzymes, (C) polyols and (D) water, wherein the content of (C) is 25 to 60% by mass relative to the total amount of the cleaning composition, the content of (D) is 5 to 30% by mass relative to the total amount of the cleaning composition, and as (C), it contains glycerol and / or sorbitol, wherein the content of glycerol and / or sorbitol is 10 to 60% by mass relative to the total amount of the cleaning composition.

[0029] (A) Higher fatty acids and / or their salts

[0030] In the cleansing compositions described in the embodiments of the present invention, component (A) can generally be used without particular limitation as long as it is a higher fatty acid and / or its salt used in topical compositions and / or cosmetics.

[0031] As component (A), examples include, for instance, higher fatty acids with 10 or more carbon atoms and / or their salts. Specific examples of component (A) are not limited to the following substances, but may include, for instance, linear saturated fatty acids such as decanoic acid, lauric acid, myristic acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, stearic acid, arachidic acid, and behenic acid; unsaturated fatty acids such as undecenoic acid, palmitoleic acid, oleic acid, linoleic acid, linolenic acid, isoleic acid, transoleic acid, ricinoleic acid, phellandrenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid; branched fatty acids such as isopalactic acid and isostearic acid; mixed fatty acids such as coconut oil fatty acids, tallow fatty acids, and palm kernel oil fatty acids; 12-hydroxystearic acid and their salts, etc. These may be used alone or in combination of two or more.

[0032] Salts of higher fatty acids are not limited to the following substances, but can include metal salts such as potassium salts, calcium salts, lithium salts, sodium salts, and magnesium salts; ammonium salts such as ammonium salts and alkylammonium salts; and amine salts such as monoethanolamine salts, diethanolamine salts, triethanolamine salts, aminomethylpropanol salts, and aminobutanetriol salts.

[0033] Of the components described above (A), from the viewpoint of significantly exerting the effects of the present invention, component (A) preferably contains one or more of the group consisting of higher fatty acids selected from those having 12 to 18 carbon atoms and their salts. From the viewpoint of further significantly exerting the effects of the present invention, component (A) preferably contains one or more of the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, and their salts. Furthermore, component (A) also preferably contains two or more of the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, and their salts, and also preferably contains three or more of the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, and their salts.

[0034] It should be noted that higher fatty acid salts can be mixed into the cleaning composition in the form of higher fatty acid salts. Alternatively, higher fatty acids and alkalis can be mixed independently to form higher fatty acid salts in the composition.

[0035] (A) The content of the component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. From the viewpoint of significantly exerting the effect of the present invention, the content relative to the total amount of the cleaning composition is preferably 10 to 40% by mass, more preferably 12 to 38% by mass, further preferably 15 to 36% by mass, and particularly preferably 20 to 35% by mass.

[0036] In the cleaning composition described in the embodiments of the present invention, when two or more of the following are selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid and their salts as component (A), the content of each of these components can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not particularly limited, for example as shown below.

[0037] The content of lauric acid and / or its salts relative to the total amount of the cleaning composition is, for example, 1–35% by mass, 2–15% by mass, 3–12% by mass, 4–10% by mass, etc. The content of myristic acid and / or its salts relative to the total amount of the cleaning composition is, for example, 1–35% by mass, 2–15% by mass, 3–12% by mass, 4–10% by mass, etc. The content of palmitic acid and / or its salts relative to the total amount of the cleaning composition is, for example, 1–35% by mass, 3–30% by mass, 5–20% by mass, 8–18% by mass, etc. The content of stearic acid and / or its salts relative to the total amount of the cleaning composition is, for example, 1–35% by mass, 1.5–30% by mass, 2–20% by mass, 3–10% by mass, etc.

[0038] (B) Protein-degrading enzymes and / or lipid-degrading enzymes

[0039] In the cleansing compositions described in the embodiments of the present invention, component (B) can generally be used without particular limitation as long as it is a protein-degrading enzyme or lipid-degrading enzyme used in topical compositions and / or cosmetics. They can be used alone or in combination of two or more.

[0040] As a protein-degrading enzyme, it can be any of the acidic, neutral, or alkaline protein-degrading enzymes. Furthermore, it can be any of the protein-degrading enzymes of animal, plant, or microbial origin. Specific examples of protein-degrading enzymes include plant-derived protein-degrading enzymes such as papain, chymopapain, bromelain, figopoietin, and pepsin; animal-derived protein-degrading enzymes such as trypsin, chymotrypsin, and pancreatin; and microbial protein-degrading enzymes produced by bacteria and other microorganisms. Examples of microbial protein-degrading enzymes include those produced by microorganisms such as Bacillus, Paenibacillus, Geobacillus, Aspergillus, Rhizopus, Rhizomucor, and Streptomyces. These can be used alone or in combination of two or more.

[0041] Among the aforementioned protein-degrading enzymes, from the viewpoint of significantly exerting the effects of the present invention, protein-degrading enzymes of microbial origin or plant origin are preferred, and protein-degrading enzymes produced by microorganisms of the genus Bacillus are more preferred.

[0042] The lipid-degrading enzyme can be any of the acidic, neutral, or alkaline lipid-degrading enzymes, and can be any of the lipid-degrading enzymes derived from animals, plants, or microorganisms. Specific examples of lipid-degrading enzymes include lipases, phospholipases, and inositol hexaphosphatase. Examples of microbial lipid-degrading enzymes include lipid-degrading enzymes produced by microorganisms such as Rhizopus, Aspergillus, Mucosum, Geotrichum, Candida, Pseudomonas, Penicillium, and Chromobacterium. These can be used alone or in combination of two or more. From the viewpoint of significantly maximizing the effects of the present invention, microbial lipid-degrading enzymes are preferred.

[0043] Commercially available products that are components (B) include, for example, "Bioplase (registered trademark) [e.g., Bioplase XL-416F]", "Protease [e.g., Protease CL-15]" (all manufactured by Nagase ChemteX); "Protease N", "Protease S", "Papain W-40" (all manufactured by Amano Pharmaceutical Co., Ltd.); "Biosoke" (manufactured by Yamato Kasei Corporation); "Alcalase (registered trademark)", "Esperase (registered trademark)", "Savinase (registered trademark)", "Durazyme (registered trademark)", "Subtilisin A" (all manufactured by Novozymes Japan Co., Ltd.); "Lilipase (registered trademark) [e.g., Lilipase A-10D]" (manufactured by Nagase ChemteX Co., Ltd.), etc.

[0044] (B) The content of the component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. From the viewpoint of significantly exerting the effect of the present invention, the enzyme activity of the mixed cleaning composition is preferably 0.01 to 5.0 U / g, more preferably 0.1 to 4.0 U / g, further preferably 0.5 to 3.0 U / g, and particularly preferably 1.0 to 2.0 U / g.

[0045] It should be noted that the "U (unit)" in the above unit "U / g" represents the activity of protein-degrading enzymes or lipid-degrading enzymes. One unit is defined as the amount of enzyme that can change 1 micromolar (μmol) of substrate per minute under optimal conditions (at a temperature of 30°C and an acidity at which the chemical reaction is most advanced).

[0046] (B) The content of the component can be appropriately set according to its enzyme activity (U / g), the type, amount, dosage form of other components, etc., without limitation, for example, 0.01-5% by mass, 0.05-3% by mass, 0.08-2% by mass, 0.1-1% by mass, etc.

[0047] (B) The component can be either an immobilized enzyme, i.e., an enzyme immobilized by means of immobilization on a carrier or the like, or an unimmobilized enzyme, i.e., an enzyme not immobilized by means of immobilization on a carrier or the like. Commonly known carriers used for enzyme immobilization can be listed as carriers. Examples include various ion exchange resins and other organic polymers, and inorganic porous materials such as ceramics. Furthermore, various known methods can be used to immobilize the enzyme. Examples include immobilization methods, inorganic carrier covalent bonding methods, organic carrier covalent bonding methods, and physical adsorption methods. This invention is particularly useful because it can effectively inhibit the decrease in enzyme activity, even for unimmobilized enzymes whose activity is easily reduced.

[0048] [(C) Polyols]

[0049] As the (C) component used in the cleansing composition according to embodiments of the present invention, polyols commonly used in topical compositions and / or cosmetics can generally be used. The number of hydroxyl groups in the (C) component is not particularly limited, but is, for example, 2 to 20 hydroxyl groups, preferably 2 to 10 hydroxyl groups, more preferably 2 to 6 hydroxyl groups, and even more preferably 2 to 4 hydroxyl groups. Furthermore, the molecular weight of the (C) component is not particularly limited, but is, for example, 50 to 1000 hydroxyl groups, preferably 50 to 600 hydroxyl groups, and more preferably 75 to 300 hydroxyl groups.

[0050] Specific examples of ingredient (C) include glycerin, sorbitol, diglyceride, triglyceride, propylene glycol, dipropylene glycol, ethylene glycol, diethylene glycol, isoprene glycol, butylene glycol, propylene glycol, xylitol, erythritol, mannitol, pentylene glycol, hexanediol, octylene glycol, decanediol, neopentylene glycol, polyethylene glycol, etc.

[0051] Examples of polyethylene glycols mentioned above include those with an average molecular weight of 150 to 1000. Specifically, examples include polyethylene glycol 200 (PEG-4), polyethylene glycol 300 (PEG-6), polyethylene glycol 400 (PEG-8), polyethylene glycol 600 (PEG-12), and polyethylene glycol 1000 (PEG-20). It should be noted that this average molecular weight can be determined using, for example, the average molecular weight test described in the polyethylene glycol section of the Pharmaceutical External Use Raw Material Standard 2006.

[0052] The above-mentioned component (C) can be used alone or in combination of two or more. From the viewpoint of achieving the effect of the present invention, the cleaning composition described in the embodiments of the present invention needs to contain glycerin or sorbitol as component (C).

[0053] Regarding the content of component (C) in the cleaning composition according to the embodiments of the present invention, from the viewpoint of achieving the effects of the present invention, it must be 25 to 60% by mass relative to the total amount of the cleaning composition, and the content of glycerol and / or sorbitol must be 10 to 60% by mass relative to the total amount of the cleaning composition.

[0054] From the viewpoint of significantly achieving the effects of the present invention, the content of glycerol and / or sorbitol relative to the total amount of the cleaning composition is preferably 12 to 55% by mass, more preferably 15 to 50% by mass, and even more preferably 18 to 45% by mass.

[0055] As component (C) in the cleaning composition according to embodiments of the present invention, from the viewpoint of further improving the effects of the present invention (especially the effect of improving water solubility), it is preferable to contain glycerin. From the viewpoint of significantly exerting the effects of the present invention (especially the effect of improving water solubility), the content of glycerin relative to the total amount of the cleaning composition is preferably 12 to 50% by mass, more preferably 14 to 45% by mass, and even more preferably 16 to 35% by mass.

[0056] As for component (C) in the cleaning composition according to embodiments of the present invention, from the viewpoint of further improving the effects of the present invention (especially improving water solubility), the content of glycerol relative to the total amount of component (C) is preferably 50% by mass or more, more preferably 60% by mass or more. There is no particular upper limit, for example, 99.8% by mass.

[0057] In the cleaning composition described in the embodiments of the present invention, component (C) may contain only glycerol and / or sorbitol. Alternatively, glycerol and / or sorbitol, along with other polyols (i.e., polyols other than glycerol and sorbitol), may be used in combination. There are no particular limitations on these other polyols. From the viewpoint of significantly enhancing the effects of the present invention, it is preferable to contain one or more from the group consisting of, for example, diglycerol, triglycerol, propylene glycol, dipropylene glycol, ethylene glycol, diethylene glycol, isoprene glycol, butylene glycol, and polyethylene glycol with an average molecular weight of 150 to 1000 (more preferably 150 to 600, and even more preferably 150 to 300). It is even more preferable to contain one or more from the group consisting of diglycerol, dipropylene glycol, and butylene glycol. Among these, from the viewpoint of further improving the effects of the present invention (especially improving water solubility), it is even more preferable to use a combination of glycerol and one or more from the group consisting of diglycerol, dipropylene glycol, and butylene glycol.

[0058] In the cleaning composition described in the embodiments of the present invention, the content of the other polyols is 0 to 50% by mass relative to the total amount of the cleaning composition. Furthermore, the content of the other polyols can be appropriately set within the above range and is not limited, for example, it can be 5 to 40% by mass, 8 to 30% by mass, 10 to 20% by mass, etc.

[0059] The total content of component (A) and component (C) [(A) + (C)] can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. From the viewpoint of significantly exerting the effect of the present invention, it is preferably 40 to 90% by mass, more preferably 45 to 80% by mass, and even more preferably 50 to 75% by mass relative to the total amount of the cleaning composition.

[0060] [(D) Water]

[0061] In the cleansing compositions described in the embodiments of the present invention, component (D) can generally be used without particular limitation as long as it is water used in topical compositions and / or cosmetics. For example, purified water is preferred.

[0062] From the viewpoint of achieving the effects of the present invention, the content of component (D) must be 5 to 30% by mass relative to the total amount of the cleaning composition. Furthermore, from the viewpoint of further improving the effects of the present invention (especially improving water solubility), the content of component (D) is preferably 10 to 30% by mass relative to the total amount of the cleaning composition, more preferably 15 to 30% by mass, and even more preferably 20 to 30% by mass.

[0063] (E) pH adjuster

[0064] In the cleaning composition described in the embodiments of the present invention, it is suitable to use a pH adjuster (E) in addition to using the components (A) to (D) described above. The pH adjuster is not particularly limited, and examples include inorganic bases such as potassium hydroxide, sodium hydroxide, and sodium carbonate; organic bases such as triethanolamine, diisopropanolamine, and triisopropanolamine; inorganic acids such as hydrochloric acid and sulfuric acid; and organic acids such as lactic acid, sodium lactate, citric acid, sodium citrate, succinic acid, and sodium succinate. Two or more of these can be used alone or in combination. Among these, potassium hydroxide and sodium hydroxide are preferred.

[0065] (E) The content of the component can be appropriately set according to the type, amount, dosage form, etc. of other components, and there is no limit. For example, it is 0.1 to 10% by mass, 0.3 to 8% by mass, or 0.5 to 7% by mass relative to the total amount of the cleaning composition.

[0066] [Other ingredients]

[0067] In the cleaning compositions described in the embodiments of the present invention, components other than those listed in (A) to (E) may be mixed in without impairing the effects of the present invention. The cleaning compositions described in the embodiments of the present invention are not limited to the following substances and may include, for example, base agents, surfactants, thickeners, preservatives / preservatives, antioxidants, chelating agents, colorants, fragrances, etc. These may be used alone or in combination of two or more.

[0068] Examples of base agents include lower alcohols such as ethanol and isopropanol; hydrocarbons such as liquid paraffin, squalane, gelled hydrocarbons (plastic matrices, etc.), ceresin, and light liquid paraffin; and methyl polysiloxane, cross-linked methyl polysiloxane, high-polymer methyl polysiloxane, cyclic organosilicon, alkyl-modified organosilicon, cross-linked alkyl-modified organosilicon, amino-modified organosilicon, polyether-modified organosilicon, polyglycerol-modified organosilicon, cross-linked polyether-modified organosilicon, cross-linked alkyl polyether-modified organosilicon, organosilicon-alkyl chain co-modified polyether-modified organosilicon, organosilicon-alkyl chain co-modified polyglycerol-modified organosilicon, and polyether-modified organosilicon. Silicone oils such as branched-chain silicones, polyglycerol-modified branched-chain silicones, acrylic silicones, phenyl-modified silicones, and silicone resins; oils such as coconut oil, olive oil, rice bran oil, shea butter, rosehip oil, and almond oil; waxes such as jojoba oil, beeswax, candelilla wax, and lanolin; higher alcohols such as cetyl alcohol, cetearyl alcohol, stearyl alcohol, behenyl alcohol, octyldodecyl alcohol, isostearyl alcohol, phytosterols, and cholesterol; dioxane; esters such as isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, isononyl isononanoate, and pentaerythritol tetra-2-ethylhexanoate. These can be used alone or in combination of two or more.

[0069] The content of the base agent can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 1 to 30% by mass, preferably 3 to 25% by mass, and more preferably 5 to 20% by mass relative to the total amount of the cleaning composition.

[0070] Examples of surfactants (excluding higher fatty acid salts) include nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and surfactants of natural origin. The content of the surfactant can be appropriately set according to the type, amount, and dosage form of other components, and is not limited. For example, it may be 1-30% by mass, 5-25% by mass, or 10-20% by mass relative to the total amount of the cleaning composition.

[0071] Examples of nonionic surfactants include: sorbitol monoisostearate, sorbitol monolaurate, sorbitol monopalmitate, sorbitol monostearate, pent-2-ethylhexanoate diglyceride, tetra-2-ethylhexanoate diglyceride, and other sorbitol fatty acid esters; lipophilic glyceryl monostearate, self-emulsifying glyceryl monostearate, glyceryl monostearate malic acid, and other glycerol fatty acid esters; polyglycerol monostearate, polyglycerol monoisostearate, polyglycerol diisostearate, polyglycerol monolaurate, polyglycerol monooleate, polyglycerol monomyristate, and other polyglycerol fatty acid esters; propylene glycol fatty acid esters such as propylene glycol monostearate; and ethylene glycol distearate, ethylene glycol monostearate, etc. Triethylene stearate, polyethylene monostearate (2E.O.), polyethylene monostearate (4E.O.), polyethylene monostearate (9E.O.), polyethylene monostearate (10E.O.), polyethylene monostearate (23E.O.), polyethylene monostearate (25E.O.), polyethylene monostearate (32E.O.) ), polyethylene monostearate (40E.O.), polyethylene monostearate (45E.O.), polyethylene monostearate (55E.O.), polyethylene monostearate (75E.O.), polyethylene monostearate (140E.O.), polyethylene distearate (140E.O.), polyethylene distearate (250E.O.).) and other ethylene glycol fatty acid esters; hydrogenated castor oil derivatives such as polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil 60 (HCO-60), and polyoxyethylene hydrogenated castor oil 80 (HCO-80); polyoxyethylene (20) sorbitol monolaurate (polysorbate 20), polyoxyethylene (20) sorbitol monostearate (polysorbate 60), polyoxyethylene (20) sorbitol monooleate (polysorbate 80), isostearate, etc. Polyoxyethylene (20) dehydrated sorbitol esters and other polyoxyethylene dehydrated sorbitol fatty acid esters; N-methylethanolamide of caprylic acid, N-methylethanolamide of decanoic acid, N-methylethanolamide of lauric acid, N-ethylethanolamide of lauric acid, N-methylpropanolamide of lauric acid, N-ethylpropanolamide of lauric acid, N-methylisopropanolamide of lauric acid, N-ethylisopropanolamide of lauric acid, N-methylethanolamide of myristic acid, N-methylethanolamide of palmitic acid, N-methylethanolamide of coconut oil fatty acid, N-ethylethanolamide of coconut oil fatty acid, coconut oil fat N-methylpropanolamide, N-ethylpropanolamide of coconut oil fatty acids, N-methylisopropanolamide of coconut oil fatty acids, N-ethylisopropanolamide of coconut oil fatty acids, N-ethylethanolamide of coconut oil fatty acids, N-methylethanolamide of palm kernel oil fatty acids, N-ethylethanolamide of palm kernel oil fatty acids, N-methylethanolamide of stearic acid, N-ethylethanolamide of stearic acid, N-methylethanolamide of oleic acid, N-ethylethanolamide of oleic acid, and other fatty acid alkanolamides; polyoxyethylene monocoaryl fatty acid glycerides; glyceryl alkyl ethers; hexyl glucoside, octyl glucoside, nonyl... Alkyl glucosides such as alkyl glucoside, decyl glucoside, lauryl glucoside, myristyl glucoside, cetearyl glucoside, hexadecyl glucoside, octadecyl glucoside, and cocoyl glucoside; polyoxyethylene lauryl ether, polyoxyethylene cetyl ether, polyoxyethylene stearyl ether, polyoxyethylene oleyl ether, and polyoxyethylene behenyl ether; and organosilicon surfactants such as lauryl PEG-9 polydimethylsiloxane ethyl dimethyl silicone oil and PEG-9 polydimethylsiloxane ethyl dimethyl silicone oil.

[0072] Among these, glycerol fatty acid esters, ethylene glycol fatty acid esters, and alkyl glucosides are preferred, and self-emulsifying glyceryl monostearate, ethylene glycol distearate, polyethylene monostearate (with an average addition of ethylene oxide of 2-400 moles, preferably 10-300, more preferably 100-150), polyethylene distearate (with an average addition of ethylene oxide of 2-400 moles, preferably 100-300), and lauryl glucoside are more preferred.

[0073] The HLB value (Hydrophile-Lipophile Balance) of the nonionic surfactant is not particularly limited, for example, it is 8 to 19, but from the viewpoint of significantly exerting the effects of the present invention, it is preferably 9 to 18, and more preferably 9.5 to 17.

[0074] The HLB value mentioned above is an indicator of the balance between hydrophilicity and lipophilicity, calculated, for example, according to the following (Equation 1) by Oda-Teramura et al.

[0075] HLB value = (ΣInorganic value / ΣOrganic value) × 10…(Equation 1)

[0076] The ΣInorganic Value / ΣOrganic Value is called IOB (Inorganic-Organic Balance). Based on the "inorganic value" and "organic value" set for each type of atom and each functional group, the "inorganic value" and "organic value" of the atoms and functional groups that constitute organic compounds such as surfactants are accumulated, and thus can be calculated (see "Organic Concept Map - Basics and Applications -" by Yoshio Koda, pp. 11-17, Sankyo Publishing, 1984).

[0077] The content of nonionic surfactant can be appropriately set according to the type, amount, dosage form, etc. of other components, and there is no limitation. Relative to the total amount of the cleaning composition, it is preferably 1-20% by mass, 1.5-15% by mass, 2-12% by mass, 2.5-10% by mass, 2.5-8% by mass, etc.

[0078] Examples of anionic surfactants include alkyl sulfates such as sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium lauryl ether sulfate, sodium myristyl sulfate, sodium stearyl sulfate, sodium oleyl sulfate, and sodium cetyl sulfate; polyoxyethylene alkyl ether sulfates such as sodium polyoxyethylene lauryl ether sulfate and sodium polyoxyethylene myristyl ether sulfate; N-acylmethyl taurates such as sodium myristoyl methyl taurate, sodium palmitoyl methyl taurate, sodium stearoyl methyl taurate, sodium oleyl methyl taurate, and sodium coconut oil fatty acid methyl taurate; alkyl sulfosuccinates such as sodium dioctyl sulfosuccinate and disodium lauryl sulfosuccinate; alkyl sulfosuccinates such as disodium polyoxyethylene lauryl sulfosuccinate; monoalkyl phosphates such as sodium lauryl phosphate, sodium cetyl phosphate, and diethanolamine cetyl phosphate; and sodium polyoxyethylene lauryl ether phosphate and sodium polyoxyethylene cetyl ether phosphate. Polyoxyethylene alkyl ether phosphate salts such as sodium wax-based ether phosphate, sodium polyoxyethylene oleyl ether phosphate, sodium polyoxyethylene alkylphenyl ether phosphate, and triethanolamine polyoxyethylene alkylphenyl ether phosphate; N-acyl sarcosine salts such as potassium lauroyl sarcosine, potassium lauroyl sarcosine, sodium myristoyl sarcosine, and sodium coconut oil fatty acid sarcosine; N-acyl-N-methyl-β-alanine salts such as sodium lauroyl methyl alanine, sodium lauroyl methyl alanine, sodium myristoyl methyl alanine, and sodium coconut oil fatty acid methyl alanine; and alkyl ether glycol acetates such as sodium lauryl glycol acetate (sodium dodecane-1,2-diol acetate), potassium lauryl glycol acetate, sodium myristoyl glycol acetate, potassium myristoyl glycol acetate, sodium palmityl glycol acetate, potassium palmityl glycol acetate, sodium stearyl glycol acetate, potassium stearyl glycol acetate, sodium behenyl glycol acetate, and potassium behenyl glycol acetate.

[0079] Examples of cationic surfactants include stearyltrimethylammonium chloride, alkyltrimethylammonium chloride, cetyltrimethylammonium chloride, behenyltrimethylammonium chloride, behenyldimethylhydroxyethylammonium chloride, stearyldimethylbenzylammonium chloride, distearatedimethylammonium chloride, dicetylmethylammonium chloride, cetyltriethylmethylammonium sulfate, stearyltrimethylammonium bromide, and cetyltrimethylammonium bromide.

[0080] Examples of amphoteric surfactants include lauryl hydroxysulfonyl betaine, lauryl ammonium betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazoline betaine, lauryl dimethylaminoacetic acid betaine, sodium N-lauroyl-N'-carboxymethyl-N'-hydroxyethyl ethylenediamine, lauryl ammonium hydroxy betaine, lauryl ammonium dimethylamine oxide, hydroxyalkyl (C12-14) hydroxyethyl sarcosine, and coconut oil fatty acid ammonium propyl betaine.

[0081] Surfactants of natural origin include, for example, lecithin, hydrogenated lecithin, saponins, sodium subtilisin, and bile acids.

[0082] Examples of thickeners include gums (gellan gum, xanthan gum, sclerotium gum, locust bean gum, biosaccharide gum, tamarind gum, quinine seed gum, gum arabic, guaiac gum, guar gum, galactose gum, tragacanth gum, etc.), carrageenan, currant gum, succinyl polysaccharide, heparin analogues, alginates (alginic acid, sodium alginate, propylene glycol alginate, etc.), agar (including agarose), gelatin, pectin, pullulan, mannan, and vinyl thickeners (polyvinyl alcohol, polyvinylpyrrolidone). Ketones, polyvinyl methyl ether, carboxyvinyl polymers, etc.), cellulose-based thickeners (methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxymethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, carboxymethylcellulose, carboxyethylcellulose, hydrophobic hydroxypropylmethylcellulose, etc.), dextran, dextrin, alkyl methacrylate copolymers, sodium polyacrylate, bentonite, dextrin fatty acid esters, distearate, lithium dimethylammonium montmorillonite, polyethylene glycol, macrogol, (hydroxyethyl acrylate / Na acryloyl dimethyl taurate) copolymer, (ammonium acryloyl dimethyl taurate / vinylpyrrolidone) copolymer, etc.

[0083] The content of the thickener can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 5% by mass relative to the total amount of the cleaning composition, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass.

[0084] Examples of preservatives / preservatives include isopropyl methylphenol, chlorobutanol, benzyl alcohol, phenethyl alcohol, benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, isobutyl p-hydroxybenzoate, isopropyl p-hydroxybenzoate, butyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, benzyl p-hydroxybenzoate, methyl p-hydroxybenzoate (methyl p-hydroxybenzoate), phenoxyethanol, ethylhexylglycerin, decanoic acid glyceride, pentylene glycol, hexanediol, and chlorophenyl glycerol ether.

[0085] The content of preservatives / preservatives can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 2.5% by mass relative to the total amount of the cleaning composition, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass.

[0086] Examples of antioxidants include butylated hydroxytoluene (BHT), butylated hydroxyanisole, sorbic acid, sodium metabisulfite, isoascorbic acid, and L-cysteine ​​hydrochloride. The content of the antioxidant can be appropriately set according to the type, amount, and dosage form of other components, and is not limited. Relative to the total amount of the cleaning composition, it is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass.

[0087] Examples of chelating agents include disodium EDTA and calcium disodium EDTA.

[0088] The content of the chelating agent can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 2.5% by mass relative to the total amount of the cleaning composition, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass.

[0089] Examples of colorants include inorganic pigments and natural pigments.

[0090] The content of the colorant can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 1% by mass relative to the total amount of the cleaning composition, preferably 0.005 to 0.1% by mass, and more preferably 0.01 to 0.05% by mass.

[0091] As a flavoring agent, examples include terpenes such as menthol, camphor syrup, borneol, geraniol, eucalyptol, anethole, limonene, and eugenol.

[0092] The content of the fragrance can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and there is no limitation. Relative to the total amount of the cleaning composition, it is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass.

[0093] The cleaning composition described in the embodiments of the present invention may contain other active ingredients without impairing the effects of the present invention. Specific examples of active ingredients are not limited to the following substances, but may include, for example, moisturizing ingredients, exfoliating agents, blood circulation promoters, astringent ingredients, ultraviolet absorbing ingredients, antibacterial ingredients, anti-inflammatory agents, vitamins, peptides or their derivatives, amino acids or their derivatives, cell activating ingredients, etc. They may be used alone or in combination of two or more.

[0094] As moisturizing ingredients, examples include natural moisturizing factors such as sodium lactate, urea, and sodium pyrrolidone carboxylate; and plant extracts such as chamomile extract, witch hazel extract, tea extract, perilla extract, coix seed extract, centella asiatica leaf extract, seaweed extract, loquat leaf extract, daisy extract, raspberry leaf extract, sea dill extract, white willow bark extract, rosemary extract, soybean extract, soybean sprout extract, tangerine peel extract, orange extract, grapefruit extract, lemon extract, strawberry geranium extract, and edelweiss extract.

[0095] The content of moisturizing ingredients can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and is not limited. For example, it is 0.001 to 20% by mass relative to the total amount of the cleansing composition, preferably 0.01 to 15% by mass, and more preferably 0.05 to 10% by mass.

[0096] Examples of exfoliating agents include almond powder, apricot shell powder, sodium chloride granules, olive pit powder, dried seawater granules, candelilla wax, walnut shell powder, cherry pit powder, coral powder, charcoal powder (tung charcoal, binchotan charcoal, bamboo charcoal, cypress charcoal, coconut shell charcoal and their activated carbon, as well as their medicinal charcoal), hazelnut shell powder, polyethylene powder, anhydrous silica, kaolin, etc.

[0097] The content of the exfoliating agent can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and is not limited. For example, it is 0.01 to 15% by mass relative to the total amount of the cleansing composition, preferably 0.05 to 10% by mass, and more preferably 0.1 to 5% by mass.

[0098] Examples of blood circulation promoters include acetylcholine, ichthammol, caffeine, capsaicin, cantharides tincture, γ-oryzanol, ginger tincture, gingerone, strychnine, Japanese swert extract, tannic acid, capsicum tincture, benzyl azoline, nicotinic acid tocopheryl ester, and benzyl nicotinic acid ester.

[0099] The content of the blood circulation promoter can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and is not limited. Relative to the total amount of the cleaning composition, it is, for example, 0.001 to 5% by mass, preferably 0.005 to 3% by mass, and more preferably 0.01 to 1% by mass.

[0100] Examples of astringent ingredients include zinc sulfate, aluminum hydroxyl, aluminum chloride, zinc phenolsulfonate, and tannic acid.

[0101] The content of the astringent component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 20% by mass relative to the total amount of the cleaning composition, preferably 0.01 to 15% by mass, and more preferably 0.1 to 10% by mass.

[0102] Examples of UV-absorbing components include octyl triazine, hexyl diethylamine benzyl benzoate, octyl dimethoxybenzyl dioxazoline propionate, 2-ethylhexyl p-methoxycinnamate, tert-butyl methoxydibenzoylmethane, phenylbenzimidazole sulfonic acid, octyl methoxycinnamate, and ethylhexyl methoxycinnamate.

[0103] The content of the ultraviolet absorbing component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 1 to 30% by mass relative to the total amount of the cleaning composition, preferably 3 to 25% by mass, and more preferably 5 to 20% by mass.

[0104] Examples of antibacterial ingredients include isopropyl cresol, chlorhexidine, benzalkonium chloride, rivanol, benzyl chloride, cresol, gluconic acid and its derivatives, povidone-iodine, potassium iodide, iodine, triclocarban, triclosan, photosensitive agent 101, photosensitive agent 201, parabens, phenoxyethanol, alkyl diaminoglycine hydrochloride, piroctone olamine salt, miconazole, etc.

[0105] The content of the antibacterial component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 2.5% by mass relative to the total amount of the cleaning composition, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass.

[0106] As anti-inflammatory agents, examples include non-steroidal anti-inflammatory agents and steroidal anti-inflammatory agents. Specifically, examples include glycyrrhizic acid, glycyrrhetinic acid, stearyl glycyrrhetinic acid ester, allantoin, ε-aminocaproic acid, salicylic acid, methyl salicylate, ethylene glycol salicylate, indomethacin, biphenylacetic acid, ibuprofen, ibuprofen pyridine methanol, ketopropionic acid, butylated hydroxy acid, butylated flufenoxuronate, bendazac, piroxicam, sulprofen, chamomile, guaiac blue, dexamethasone valerate, dexamethasone, prednisolone valerate (prednisolone valerate acetate), prednisolone acetate, prednisolone, hydrocortisone acetate, hydrocortisone, urfenadine, butylated hydroxy acid, and their salts.

[0107] The content of the anti-inflammatory agent can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and is not limited. For example, it is 0.001 to 5% by mass relative to the total amount of the cleaning composition, preferably 0.005 to 4.5% by mass, and more preferably 0.01 to 3% by mass.

[0108] Examples of vitamins include vitamin E derivatives such as dl-α-tocopherol, d-δ-tocopherol, dl-α-tocopherol succinate, and calcium dl-α-tocopherol succinate; vitamin B2 derivatives such as riboflavin, flavin mononucleotide, flavin adenine dinucleotide, riboflavin butyrate, riboflavin tetrabutyrate, sodium riboflavin 5'-phosphate, and riboflavin tetranicotinate; and nicotinic acid dl-α-tocopherol ester, benzyl nicotinate, methyl nicotinate, β-butoxyethyl nicotinate, and 1-(4-methylphenyl)ethyl nicotinate. Niacin derivatives, ascorbic acid precursor-A, L-ascorbic acid 2-glucoside, ascorbic acid stearate, ascorbic acid palmitate, dipalmitic acid L-ascorbic acid and other vitamin C derivatives, methyl hesperidin, ergocalciferol, cholecalciferol and other vitamin D derivatives, chlorophyllin, farnesin and other vitamin K derivatives, γ-oryzanol, dibenzoylthiamine, dibenzoylthiamine hydrochloride, thiamine hydrochloride, thiamine cetyl hydrochloride, thiamine thiocyanate, thiamine lauryl hydrochloride, thiamine nitrate, sulfur Thiamine monophosphate, thiamine lysine salt, thiamine triphosphate, thiamine monophosphate phosphate, thiamine monophosphate, thiamine diphosphate, thiamine diphosphate hydrochloride, thiamine triphosphate, thiamine triphosphate monophosphate and other vitamin B1 derivatives; pyridoxine hydrochloride, pyridoxine acetate, pyridoxal hydrochloride, pyridoxal 5'-phosphate, pyridoxamine hydrochloride and other vitamin B6 derivatives; cyanocobalamin, hydroxycobalamin, deoxyadenosylcobalamin and other vitamin B12 derivatives; folic acid, pteroylglutamic acid and other folic acid derivatives; niacin, niacinamide. Vitamins containing niacin, pantothenic acid, calcium pantothenate, panthenol, D-panthenyl thioethylamine, D-panthenyl thioethylamine, coenzyme A, pantothenic acid ether, biotin, biocytin, ascorbic acid, sodium ascorbate, dehydroascorbic acid, sodium ascorbate phosphate, magnesium ascorbate phosphate, retinol, hydrogenated retinol, retinyl palmitate, carnitine, ferulic acid, alpha-lipoic acid, orotic acid, etc.

[0109] The content of vitamins can be appropriately set according to the type, amount, dosage form, etc. of other ingredients, and there is no limitation. Relative to the total amount of the cleaning composition, it is, for example, 0.001 to 25% by mass, preferably 0.01 to 20% by mass, and more preferably 0.05 to 15% by mass.

[0110] Examples of peptides or their derivatives include keratin-degrading peptides, hydrolyzed keratin, collagen, fish collagen, determinated collagen, gelatin, elastin, elastin-degrading peptides, collagen-degrading peptides, hydrolyzed collagen, hydroxypropyl ammonium chloride hydrolyzed collagen, elastin-degrading peptides, shellac-degrading peptides, hydrolyzed shellac, silk protein-degrading peptides, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soybean protein-degrading peptides, hydrolyzed soybean protein, wheat protein, wheat protein-degrading peptides, hydrolyzed wheat protein, casein-degrading peptides, and acylated peptides (palmitoyl oligopeptides, palmitoyl pentapeptides, palmitoyl tetrapeptides, etc.).

[0111] The content of peptides or their derivatives can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 5% by mass relative to the total amount of the cleaning composition, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass.

[0112] Examples of amino acids or their derivatives include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, creatine, etc.

[0113] The content of amino acids or their derivatives can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 5% by mass relative to the total amount of the cleaning composition, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass.

[0114] As cell-activating components, examples include α-hydroxy acids such as glycolic acid and lactic acid; tannins, flavonoids, saponins, and photosensitizer 301.

[0115] The content of the cell-activating component can be appropriately set according to the type, amount, dosage form, etc. of other components, and is not limited. For example, it is 0.001 to 5% by mass relative to the total amount of the cleaning composition, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass.

[0116] [pH of the cleaning composition]

[0117] The pH of the cleaning composition described in the embodiments of the present invention can be appropriately set according to the dosage form, etc., and is not limited thereto. For example, the pH is preferably 8.0 to 12.0. In addition, regarding the pH of the cleaning composition described in the embodiments of the present invention, from the viewpoint of inhibiting the decrease of enzyme activity, the pH is more preferably 9.0 to 11.5, and the pH is even more preferably 9.0 to 11.0.

[0118] It should be noted that the pH values ​​mentioned above were measured using a pH meter (e.g., pH METER F-52 (manufactured by HORIBA)) at room temperature (23°C).

[0119] [Viscosity]

[0120] The viscosity (25°C) of the cleaning composition described in the embodiments of the present invention can be appropriately set according to the dosage form, etc., and is not limited. From the viewpoint that it is easy to discharge when filled into a tubular container and will not drip from fingertips or palms and easily foam, it is preferably 30,000 to 2,000,000 mPa·s, more preferably 60,000 to 1,800,000 mPa·s, further preferably 100,000 to 1,500,000 mPa·s, even more preferably 150,000 to 1,300,000 mPa·s, and most preferably 150,000 to 1,000,000 mPa·s.

[0121] The viscosity (25°C) mentioned above refers to the viscosity measured using a single-cylinder rotational viscometer (Brookfield type viscometer) according to the viscosity determination method described in the 17th revised edition of the General Test Methods of the Japanese Pharmacopoeia. Specifically, it refers to the value measured using a TV-10M (manufactured by Toki Sangyo Co., Ltd.), with the rotor, rotational speed, and other conditions selected according to the machine's instruction manual, at 25°C. More specifically, the viscosity at 25°C was measured using an M4 rotor at a rotational speed of 0.3 rpm and a measurement time of 180 seconds.

[0122] The following describes the relevant information about a single-cylinder rotational viscometer. A single-cylinder rotational viscometer measures the torque required to rotate a cylinder within a liquid at a specified angular velocity. The apparatus constant KB is experimentally determined beforehand using a standard solution for viscometer calibration, and the viscosity η of the liquid is then calculated using the following formula.

[0123] η=KB×T / ω

[0124] η: Viscosity of the liquid (mPa·s)

[0125] KB: Device constant (rad / cm) 3 )

[0126] ω: Angular velocity (rad / s)

[0127] T: Torque acting on the cylindrical surface (10 -7 N·m)

[0128] <The usefulness of this invention>

[0129] When proteolytic and lipolytic enzymes are stored in the presence of water for a certain period, their activity decreases or they become inactive. Therefore, to maintain enzyme activity in cleaning compositions, it is usually necessary to drastically reduce the water content in the cleaning composition. However, drastically reducing the water content can lead to decreased affinity between the cleaning composition and water, reduced water solubility during use, or the cleaning composition itself becoming too rigid, resulting in reduced dischargeability when filling tubular containers, and sometimes deteriorating usability. Therefore, it is extremely difficult to provide a cleaning composition that both maintains the enzyme activity of proteolytic and lipolytic enzymes and offers excellent usability.

[0130] The cleaning composition described in the embodiments of the present invention is useful because it solves the above-mentioned difficult problems and provides a cleaning composition that can inhibit the decrease of enzyme activity of protein-degrading enzymes and lipid-degrading enzymes, stably maintain enzyme activity, and has excellent usability.

[0131] Specifically, the cleansing composition described in the embodiments of the present invention, by mixing in higher fatty acids and / or their salts, specific polyols, and water while also mixing in proteolytic enzymes and lipolytic enzymes, and controlling the content of the polyols and water within a specific range, can inhibit the decrease in the enzyme activity of proteolytic enzymes and lipolytic enzymes, and stably maintain enzyme activity. Therefore, it can effectively cleanse the skin by breaking down keratin and sebum. That is, the cleansing composition described in the embodiments of the present invention can maintain the enzyme activity of proteolytic enzymes and lipolytic enzymes even after a specified period following manufacturing, for example, resulting in excellent cleansing effects such as keratin removal and sebum removal during use.

[0132] Furthermore, the cleaning composition described in the embodiments of the present invention has good water solubility, for example, during use, and therefore has excellent usability.

[0133] From the viewpoint of further enhancing the usefulness of the present invention, as a preferred embodiment, examples include setting the pH of the cleansing composition to be near the optimal pH of component (B). This results in superior cleansing effects such as exfoliation and sebum removal.

[0134] It should be noted that the optimal pH range for component (B) mentioned above refers to the pH of the washing composition being ±2.0, preferably ±1.5, and more preferably ±1.0 of the optimal pH of component (B). Furthermore, the optimal pH of component (B) refers to the pH at which the enzyme activity exhibits its maximum value, and can be determined using known methods. Not limited to the following methods, for example, the optimal pH can be defined as follows: Adding 1 mL of component (B) (e.g., Bioplase XL-416F (manufactured by Nagase ChemteX)) relative to 5 mL of 0.6% by mass casein, adjusting the pH to between 4.0 and 12.0 using a pH adjuster, and reacting at 30°C for 10 minutes, results in the highest relative enzyme activity at that pH.

[0135] Furthermore, from the viewpoint of further enhancing the usefulness of the present invention, as a preferred embodiment, an example can be provided where an alkaline proteolytic enzyme (alkaline protease) and / or an alkaline lipolytic enzyme (alkaline lipase) are included as component (B), and the pH of the cleansing composition is set to 9.0 or higher. This results in superior cleansing effects such as exfoliation and sebum removal. It should be noted that alkaline proteolytic enzyme (alkaline protease) refers to a proteolytic enzyme whose optimal pH is in the alkaline region, for example, a proteolytic enzyme with an optimal pH of 9.0 or higher. Similarly, alkaline lipolytic enzyme (alkaline lipase) refers to a lipolytic enzyme whose optimal pH is in the alkaline region, for example, a lipolytic enzyme with an optimal pH of 9.0 or higher. As a more preferred embodiment of the present invention, an example can be provided where an alkaline proteolytic enzyme and / or an alkaline lipolytic enzyme with an optimal pH of 9.0 to 11.0 are used, and the pH of the cleansing composition is set to 9.0 to 11.0.

[0136] In the cleaning composition described in the embodiments of the present invention, the effect of inhibiting the decrease in enzyme activity of proteolytic enzymes and lipidolytic enzymes can be evaluated, for example, by comparing the enzyme activity measured immediately after the preparation of the cleaning composition with the enzyme activity measured after a specified storage period. As a specific example, the evaluation can be performed using the method described in the following examples. As for the cleaning composition described in the embodiments of the present invention, the enzyme activity residual rate determined according to or based on the method described in the following examples is preferably 70% or more, more preferably 80% or more, and even more preferably 90% or more.

[0137] [Formulation Form]

[0138] The cleaning composition described in the embodiments of the present invention is prepared into various forms by mixing the above-mentioned components and, as needed, further components, according to conventional methods. The formulation form is not particularly limited and can be any dosage form such as bath soap, hand soap, facial cleanser, or shampoo. Furthermore, it can be in the form of cleansing water, rinse-in-shampoo, or conditioner. Additionally, the cleaning composition described in the embodiments of the present invention can be in any form such as gel or paste.

[0139] [use]

[0140] The cleansing compositions described in the embodiments of the present invention are suitable for use as skin cleansing compositions and hair cleansing compositions. In particular, the cleansing compositions described in the embodiments of the present invention are useful for removing bodily fluids such as oil and sweat, and dirt from the skin (including the scalp), and are especially suitable for use as facial cleansing compositions (facial washes). As a method of using the cleansing compositions described in the embodiments of the present invention, for example, the cleansing composition is lathered with water in the palm of the hand and applied to the skin.

[0141] [container]

[0142] The cleaning composition described in the embodiments of the present invention can be filled into a suitably selected container for use, depending on the intended purpose and application. Examples of such containers include bottle-type, tube-type, can-type, dispenser-type, soft pouch, and spout pouch. Furthermore, examples of materials constituting the container include polyethylene terephthalate, polypropylene, polyethylene (HDPE, LDPE, LLDPE, etc.), ABS resin, ethylene vinyl alcohol resin, polystyrene, glass, and metals (aluminum, etc.) and mixtures thereof. Additionally, containers formed from these materials can be designed with considerations such as strength, flexibility, weather resistance, and component stability, for example, by applying various coating treatments to the container surface. Furthermore, containers can also be formed by laminating films or the like made from the aforementioned materials.

[0143] Among these various containers, those that allow the contents (cleaning composition) to be squeezed out by the user holding the container and applying pressure are preferred; for example, tubular containers and bottle-type containers are preferred. From the viewpoint of significantly maximizing the effects of the present invention, tubular containers are particularly preferred. Known tubular containers can be used as tubular containers (for example, without limitation, see Japanese Patent Application Publication No. 2000-272643, Japanese Patent Application Publication No. 2004-148628, Japanese Patent Application Publication No. 2005-022682, etc.). A specific example of a tubular container is a container having a body and a cap for holding the cleaning composition. This body has: a bottle portion, which is formed by rolling a single layer or laminate of synthetic resin such as polyethylene (the total thickness of the sheet is, for example, about 0.1 to 1 mm), overlapping and heat-sealing the two ends to form a cylindrical shape; a bottom sealing portion, which is formed by heat-sealing one end of the bottle portion into a flat shape; and a head, which is provided at the other end of the bottle portion. The head has a shoulder and a discharge port, the discharge port being located approximately in the center of the shoulder and covered by a cap in a freely opening and closing manner. The user can squeeze out the contents (cleaning composition) of the container by holding the bottle body and applying pressure, thus providing a cleaning composition with excellent usability, which is extremely useful. Furthermore, the cleaning composition filled into the tubular container also excels in barrier properties and / or hygiene, and is lightweight and portable, making it extremely useful from these perspectives as well.

[0144] Methods to Inhibit Decreased Enzyme Activity

[0145] As another embodiment of the present invention, a method for inhibiting the reduction of enzyme activity may also be suitably provided. For example, a method for inhibiting the reduction of enzyme activity may be suitably provided, wherein the method for inhibiting the reduction of enzyme activity by mixing (A) higher fatty acids and / or their salts, (B) proteolytic enzymes and / or lipolytic enzymes, (C) polyols, and (D) water includes the following steps: adjusting the content of the polyol (C) to 25-60% by mass relative to the total amount of the cleaning composition, and adjusting the content of glycerol and / or sorbitol (C) to 10-60% by mass relative to the total amount of the cleaning composition; and adjusting the content of water (D) to 5-30% by mass relative to the total amount of the cleaning composition.

[0146] <Manufacturing Method>

[0147] The method for manufacturing the cleaning composition described in the embodiments of the present invention is not particularly limited, and it can be manufactured by conventional methods. For example, it can be manufactured by adding (A) to (D), other ingredients as needed, and mixing them together.

[0148] As a suitable manufacturing method for the cleaning composition described in embodiments of the present invention, examples include, for instance, a manufacturing method that includes at least one step of mixing / stirring phase I containing (C) and (D) with phase II containing (A) and cooling. Specifically, a suitable manufacturing method is, for example, one that includes the step of adding phase II containing (A) dissolved by heating at 60 to 80°C to phase I containing (C) and (D) heated to 60°C or higher, stirring, and then adding (B) at 55°C or lower after a cooling step.

[0149] More specifically, phase I is first prepared by heating (C) and (D) to above 60°C. This heating temperature can be appropriately set within a range above 60°C, for example, preferably 60–85°C, more preferably 70–80°C. It should be noted that phase I may contain components other than (C) and (D).

[0150] Next, phase II is prepared by heating and dissolving (A). The heating temperature can be appropriately set in the range of 60–80°C, preferably 70–80°C. It should be noted that phase II may contain components other than (A).

[0151] Relative to phase I obtained above, phase II is added and stirred. After cooling the resulting mixture, (B) is added. Specifically, for example, phase I is stirred while phase II is slowly added and stirred. After cooling the resulting mixture to below 55°C, (B) is added while stirring the mixture. The aforementioned cooling temperature can be appropriately set within the range of below 55°C. From the viewpoint of significantly exerting the effects of the present invention, 30 to 55°C is preferred, and more preferably 32 to 45°C. After adding (B), the mixture is stirred until homogeneous to obtain the cleaning composition described in the embodiment of the present invention.

[0152] Example

[0153] The present invention will now be described in more detail, but it is not limited thereto. It should be noted that the units of content in Tables 1 and 2, and the formulation examples, are % by mass, and are all values ​​converted from pure mass. Furthermore, the total amount of each composition is 100% by mass.

[0154] The cleaning compositions (Examples 1-9, Comparative Examples 1-5) with the formulations shown in Tables 1 and 2 below were prepared using conventional methods, and their enzyme activity and usability were evaluated as described below.

[0155] [Table 1]

[0156]

[0157] [Table 2]

[0158] (quality%)

[0159]

[0160] ※1 Bioprase XL-416F

[0161] (Manufactured by NagaseChemteX, 20% pure ingredient. The content shown in the table refers to the content of protein-degrading enzymes as pure ingredients.)

[0162] ※2 Plantacare 1200UP

[0163] (Made by BASF, 52% pure ingredient content. The content shown in the table refers to the content of lauryl glucoside as a pure ingredient.)

[0164] ※3RHEODOL MS-165V (Manufactured by Kao Corporation)

[0165] <Enzyme Activity Evaluation Test>

[0166] Using the Pierce Protease Assay Kit (manufactured by Thermo Fisher Scientific), the enzyme activity after storage at 40°C for one month was evaluated according to the prescribed protocol.

[0167] First, prepare the following: a quantitative buffer (50 mM borate, pH 8.5), a succinylated casein solution obtained by dissolving 10 mg of freeze-dried succinylated casein in 5 mL of the quantitative buffer, and a TNBSA diluted standard solution (TNBSA Working Solution) obtained by adding 100 μL of TNBSA (trinitrobenzenesulfonic acid) stock solution to 14.9 mL of the quantitative buffer.

[0168] Next, 50 μL of the washing composition from Example 1 was added to 100 μL of the quantitative buffer solution and mixed. After standing at 25°C for 20 minutes, 50 μL of TNBSA diluted standard solution was added and mixed. The mixture was then stood at 25°C for another 20 minutes to prepare the control sample. The absorbance at 450 nm was measured using an absorbance meter (SH-9000Lab, CORONA ELECTRIC). CT ).

[0169] In addition, relative to 100 μL of the succinylated casein solution mentioned above, 50 μL of the washing composition from Example 1 was added and mixed. After standing at 25°C for 20 minutes, 50 μL of TNBSA diluted standard solution was added and mixed. After standing at 25°C for another 20 minutes, the sample for measurement was prepared. Using an absorbance meter (SH-9000Lab, CORONA ELECTRIC), the absorbance at 450 nm immediately after preparing the sample (A1) and the absorbance at 450 nm after storage at 40°C for one month (A2) were measured.

[0170] The residual enzyme activity of Example 1 was calculated using the following formula and evaluated according to the following criteria. The results are shown in Table 1.

[0171] [Formula] Enzyme activity residual rate (%) = [A2 - A] CT ] / [A1-A CT ]×100

[0172] (Evaluation Criteria)

[0173] ◎(Excellent)...Enzyme activity residual rate is over 90%

[0174] (Excellent)...Enzyme activity residual rate is above 70% and less than 90%.

[0175] △ (Good)...Enzyme activity residual rate is 50% or more but less than 70%.

[0176] × (Poor)...Enzyme activity residual rate less than 50%

[0177] The enzyme activity residual rates of Examples 2-9 and Comparative Examples 1-5 were evaluated using the same method as described above. The results are shown in Tables 1 and 2.

[0178] <Usability Evaluation Test (Water Solubility)>

[0179] 1 g of the cleaning composition from Example 1 was added to a 20 mL beaker, followed by 10 g of water (23°C). A tetrafluoroethylene resin rotor (5 mm in diameter, 15 mm in total length, manufactured by ASONE) was added, and the mixture was stirred at 300 rpm using a magnetic stirrer (HE-16GX6, manufactured by Koike Precision Machinery Co., Ltd.). The water solubility was evaluated according to the following evaluation criteria. The results are shown in Table 1.

[0180] It should be noted that the dissolution time in the following evaluation criteria refers to the time from the start of the above stirring until the clumps of the washing composition disappear visually.

[0181] (Evaluation Criteria)

[0182] ◎(Excellent)...Dissolving time less than 5 minutes

[0183] (Excellent)... Dissolving time is more than 5 minutes but less than 10 minutes.

[0184] △ (Good) ... Dissolving time is more than 10 minutes

[0185] The water solubility of Examples 2-9 was evaluated using the same method as described above. The results are shown in Tables 1 and 2. It should be noted that when water solubility was not evaluated, it was indicated by "-" in the tables.

[0186] As shown in Tables 1 and 2, in Examples 1 to 9 of the present invention, the enzyme activity residual rate was high after being stored at 40°C for 1 month, confirming that the enzyme activity was maintained.

[0187] On the other hand, in Comparative Examples 1 and 2 with low glycerol and / or sorbitol content, Comparative Examples 3 and 4 with high water content, and Comparative Example 5 with low polyol content and high water content, the enzyme activity residual rate was significantly reduced after storage at 40°C for 1 month, confirming that the enzyme activity was not sustained.

[0188] As shown in Tables 1 and 2, it can be confirmed that Examples 1 to 9 of the present invention have excellent water solubility.

[0189] In summary, it can be confirmed that, as in this invention, a cleaning composition containing (A) higher fatty acids and / or their salts, (B) proteolytic enzymes and / or lipolytic enzymes, (C) polyols, and (D) water, wherein the content of (C) is 25-60% by mass relative to the total amount of the cleaning composition, the content of (D) is 5-30% by mass relative to the total amount of the cleaning composition, and glycerol and / or sorbitol are contained as (C) and the content of glycerol and / or sorbitol is 10-60% by mass relative to the total amount of the cleaning composition, can inhibit the reduction of enzyme activity of (B) proteolytic enzymes and lipolytic enzymes. Furthermore, it has been confirmed that the cleaning composition of this invention has excellent water solubility.

[0190] It should be noted that the enzyme activity (U / g) of the proteolytic enzyme in the compositions of Examples 1-9 is 1.5 (U / g). This enzyme activity (U / g) is measured as follows: when 1 mL of (B) proteolytic enzyme [Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX)] is added to 5 mL of 0.6% casein (pH 7.5, M / 25 phosphate buffer), and the mixture is reacted at 30°C for 10 minutes, the activity equivalent to the release of 1 μg of tyrosine in furin within 1 minute as a TCA (trichloroacetic acid) soluble component is defined as 1 unit.

[0191] The following are examples of formulations of the present invention.

[0192] It should be noted that all formulations maintained enzyme activity, demonstrating good enzyme activity.

[0193] <Facial Cleanser 1 (Face Cleanser filled into the tube container)>

[0194]

[0195]

[0196] ※Protein-degrading enzyme: Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX)

[0197] ※Lipid-degrading enzyme: Lilipase (registered trademark) A-10D (manufactured by Nagase ChemteX)

[0198] <Facial Cleanser 2 (Refill the facial cleanser into the tube container)>

[0199]

[0200]

[0201] ※Protein-degrading enzyme: Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX)

[0202] ※Lipid-degrading enzyme: Lilipase (registered trademark) A-10D (manufactured by Nagase ChemteX)

[0203] <Face Cleanser 3 (Face Cleanser filled into the tube container)>

[0204]

[0205]

[0206] ※Protein-degrading enzyme: Bioplase XL-416F (manufactured by Nagase ChemteX)

[0207] <Face Cleanser 4 (Face Cleanser filled into the tube container)>

[0208]

[0209]

[0210] ※Protein-degrading enzyme: Bioplase XL-416F (manufactured by Nagase ChemteX)

[0211] <Face Cleanser 5 (Face Cleanser filled into the tube container)>

[0212]

[0213] ※Protein-degrading enzyme: Bioplase XL-416F (manufactured by Nagase ChemteX)

[0214] <Facial Cleanser 6 (Face Cleanser to be filled into the tube container)>

[0215]

[0216]

[0217] ※Protein-degrading enzyme: Bioplase XL-416F (manufactured by Nagase ChemteX)

[0218] Liquid Bath Soap

[0219]

[0220]

[0221] ※Protein-degrading enzyme: Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX)

[0222] The above embodiments and formulation examples illustrate specific forms of the present invention, but these embodiments and formulation examples are merely illustrative and not intended to be limiting. It should be considered that various modifications that are obvious to those skilled in the art fall within the scope of the present invention.

[0223] Industrial availability

[0224] The cleansing compositions of the present invention are suitable for use as cleansing compositions for skin and hair. In particular, they are suitable for use as facial cleansing compositions (facial washes).

Claims

1. A cleaning composition comprising: (A) Higher fatty acids and / or their salts, (B) Protein-degrading enzymes and / or lipid-degrading enzymes, (C) Polyols, of which, Polyethylene glycol is limited to polyethylene glycol with an average molecular weight of 150-600, and (D) Water, The content of (C) is 25-60% by mass relative to the total amount of the cleaning composition. The content of (D) is 5-30% by mass relative to the total amount of the cleaning composition. As stated in (C), it contains glycerol. The content of glycerol is 10-60% by mass relative to the total amount of the cleaning composition. The pH of the cleaning composition is 8.0~11.

5.

2. The cleaning composition according to claim 1, wherein, The (A) is a higher fatty acid with 12 to 18 carbon atoms and / or its salt.

3. The cleaning composition according to claim 1 or 2, wherein, The (B) is a protein-degrading enzyme and / or lipid-degrading enzyme in an unimmobilized state.

4. The cleaning composition according to claim 1 or 2, wherein, The (B) is an alkaline protein-degrading enzyme and / or an alkaline lipid-degrading enzyme.

5. The cleaning composition according to claim 1 or 2, wherein, The content of (D) is 15 to 30% by mass relative to the total amount of the cleaning composition.

6. The cleaning composition according to claim 1 or 2, further comprising (E) a pH adjuster.

7. The cleaning composition according to claim 1 or 2, which is filled into a tubular container.

8. A method for manufacturing the cleaning composition according to any one of claims 1 to 7, comprising the following steps: Phase II, which contains (A) that has been dissolved by heating at 60-80°C, is added to Phase I, which contains (C) and (D) heated to above 60°C, and stirred. After a cooling process, (B) is added at below 55°C.

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