一种具有金属钴抗性的酿酒酵母及其在清除污水中重金属钴方面的应用

By constructing a Saccharomyces cerevisiae strain lacking the GIS2, ISU1, or MRX14 genes, its adsorption capacity for cobalt ions was enhanced, solving the problem of removing heavy metal cobalt from water bodies and achieving a highly efficient cobalt pollution control effect.

CN117550722BActive Publication Date: 2026-07-17WUXI QIAOYI ANCIENT CANAL MIDDLE SCHOOL +3

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUXI QIAOYI ANCIENT CANAL MIDDLE SCHOOL
Filing Date
2023-10-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies are ineffective at removing the heavy metal cobalt from water, leading to environmental pollution and health risks, and traditional methods are inefficient.

Method used

By screening and constructing Saccharomyces cerevisiae strains that lack the GIS2, ISU1, or MRX14 genes, their tolerance and adsorption capacity for cobalt ions are enhanced. These gene-deficient yeast strains are then used to adsorb cobalt ions in water, thereby reducing the heavy metal content in the water.

Benefits of technology

It significantly improves the adsorption rate of cobalt ions, reaching up to 91.9% under high concentration conditions, effectively reducing the cobalt content in the aquatic environment and providing an efficient solution for cobalt pollution control.

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Abstract

本发明公开了一种具有金属钴抗性的酿酒酵母及其在清除污水中重金属钴方面的应用,属于环境工程技术领域。本发明通过高通量方法,对文库中以BY4743为母菌株的4800多个单基因缺失株进行筛选,验证了gis2缺失株对钴离子的耐受性。本发明进一步提供了GIS2基因缺失菌株在减少水环境中钴含量方面的新用途,在含有120mg / L钴的模拟污水体系中,gis2缺失株的重金属钴吸附率达91.9%。在含有240mg / L钴的模拟污水体系中,gis2缺失株重金属钴吸附率达65%。本发明提供的钴抗性菌株可直接应用于吸附环境中的重金属钴,并有助于开发清除环境水质中重金属钴的高工程菌株。
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