南五味子的特征图谱的构建方法和其应用

By using a gradient elution method with a mobile phase of acetonitrile and isopropanol mixture, the problems of long detection time and high cost of Schisandra chinensis were solved, enabling rapid and accurate quality assessment and identification of Schisandra chinensis, and improving the service life and detection efficiency of the chromatographic column.

CN117571908BActive Publication Date: 2026-07-17GUANGZHOU BAIYUNSHAN ZHONGYI PHARMACEUTICAL CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU BAIYUNSHAN ZHONGYI PHARMACEUTICAL CO LTD
Filing Date
2023-11-30
Publication Date
2026-07-17

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Abstract

本申请涉及中药检测技术领域，特别涉及一种南五味子的特征图谱的构建方法和其应用，该方法采用了如下液相色谱检测的条件：（1）流动相A为乙腈，流动相B为水和异丙醇的混合液，水和异丙醇的体积比为（9~10）：1；（2）梯度洗脱，包括：0~14.5min，维持流动相A的体积百分比为45%；14.5min~19.4min，流动相A的体积百分比由45%上升至60%；19.4min~38min，维持流动相A的体积百分比为60%。本申请的液相色谱检测方法所需时间短、试剂使用量少，制得的特征图谱基线平稳、特征峰分离度好、干扰峰的影响小，可用于南五味子的鉴别和指标成分的含量测定以及质量评估。
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