A blood lymph collector for small shrimps and a method of use

Abstract

The application discloses a blood lymph collector for small shrimps and a use method, relates to the technical field of blood lymph collection, and comprises a plug head, a collector main body and a collecting needle head. One end of the plug head is connected with a collecting tube converging port. The collector main body is connected at one end of the collecting tube. The collector main body is composed of multiple groups of sampling tubes. One end of the collector main body, which is away from the collecting tube converging port, is connected with a tail end converging port. The top ends of the multiple groups of sampling tubes converge at the collecting tube converging port. The tail ends of the multiple groups of sampling tubes converge at the tail end converging port. The collecting needle head is connected at one end of the tail end converging port, which is away from the sampling tubes. A liquid inlet is arranged at the top of the plug head. The collector is quickly and stably inserted into the shrimp at the linking position between the base of the last pair of walking legs and the abdomen, and the blood lymph of the shrimp is collected. Moreover, the blood lymph can be quickly mixed with an anticoagulant after the collection is completed.

Description

Technical Field

[0001] This invention relates to the field of hemolymph collection technology, specifically to a hemolymph collector for small shrimp and its usage method. Background Technology

[0002] Shrimp are an important type of aquaculture animal, and the study of shrimp hemolymph is an important research direction in aquaculture. However, for small shrimp, the hemolymph content is relatively low, making hemolymph collection extremely difficult, and consequently, it is difficult to obtain tissue samples that meet experimental requirements.

[0003] Currently, the main method for extracting hemolymph from shrimp is using syringes. However, syringes cannot meet the requirements for collecting hemolymph from small (small-sized) shrimp, and syringe-collected hemolymph has many limitations. For example, it requires a high level of operational skill, which is generally beyond the reach of graduate students or laboratory personnel; during collection, a significant amount of hemolymph is lost instantly when the shrimp's coelomic sinuses are punctured, resulting in insufficient collection and ultimately failing to meet research requirements. Therefore, a hemolymph collection device that is suitable for small shrimp, easy to operate, and minimizes hemolymph loss is urgently needed.

[0004] In view of this, and in view of the shortcomings of existing sampling equipment and their use, a hemolymph collector for small shrimp is proposed to achieve a more practical purpose. Summary of the Invention

[0005] The purpose of this invention is to provide a hemolymph collector for small shrimp, in order to solve the problems mentioned in the background art that the syringe cannot meet the requirements for hemolymph collection from small shrimp, and that the hemolymph collected by the syringe has many limitations.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a hemolymph collector for small shrimp, comprising a plug head, a collector body, and a collection needle. One end of the plug head is connected to a collection tube constriction port, and the collector body is connected to the end of the collection tube constriction port away from the plug head. The collector body is composed of multiple sets of sampling tubes, and the end of the collector body away from the collection tube constriction port is connected to a tail end constriction port. The top ends of the multiple sets of sampling tubes are constricted at the collection tube constriction port, and the tail ends of the multiple sets of sampling tubes are constricted at the tail end constriction port. The collection needle is connected to the end of the tail end constriction port away from the sampling tubes. A liquid inlet is provided at the top of the plug head, and the liquid inlet communicates with the collection tube body and the collection needle.

[0007] Preferably, the syringe contains an appropriate amount of anticoagulant.

[0008] Preferably, the inner diameter of the sampling tube is 0.75-2.0 mm, and the length of the sampling tube is 2-10 cm. The main body of the collector is composed of multiple sets of sampling tubes, and the multiple sets of sampling tubes are arranged in parallel. The sampling needle and the multiple sampling tubes in the main body of the collector are integrally formed.

[0009] Preferably, the collection needle and sampling tube are made of common materials used in capillary production, such as glass, and the plug is made of materials such as plastic or stainless steel. The collection needle and sampling tube form a 135-degree obtuse angle.

[0010] Preferably, the liquid inlet on the plug head is fitted into the connecting needle tube on the injection needle head.

[0011] Preferably, one structure of the sampling needle is that the tail ends of multiple sampling tubes converge into a single No. 1 needle, and the No. 1 needle and the multiple sampling tubes are all connected.

[0012] Preferably, one structure of the sampling needle is that the tail end of multiple sampling tubes is provided with an independent No. 2 needle, and the multiple sets of No. 2 needles are arranged side by side to form a sampling needle.

[0013] The method of using the collector of this invention is as follows: the collection needle is quickly and stably inserted into the junction of the base of the last pair of walking legs on the ventral side of the shrimp and the abdomen, and the hemolymph of the shrimp is collected. The inner wall of the collector body is pre-wetted with anticoagulant. When the collection needle is inserted into the shrimp body, the lymph fluid will automatically enter the collector body due to capillary action. After collection is completed, the lower end connector of the syringe with pre-stored anticoagulant is inserted into the plunger of the collector. The syringe is pushed, and the anticoagulant flows into the plunger through the inlet and enters the collector body through the collection tube constriction port. The anticoagulant is used to squeeze the hemolymph in each sampling tube and inject it into the sample tube.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention uses multiple sampling tubes, with both ends converging through the collection tube converging port and the tail converging port respectively. This allows the main body of the collector to have a large capacity while keeping the volume at both ends smaller, making it easier to connect to other components and penetrate the shrimp's body. The combination structure and size of the collection needle and the main body of the collector enable effective collection of hemolymph from shrimp, minimizing wounds and hemolymph loss. After collection, the connector of the syringe pre-loaded with anticoagulant is... Insert the syringe plunger into the inlet, then place the No. 1 needle of the sampler into the opening of the sample tube. By pushing the syringe piston, inject the anticoagulant into the sampler. The anticoagulant flows into the plunger through the inlet and enters the main body of the sampler through the collection tube constriction. The anticoagulant squeezes the hemolymph in each sampling tube into the sample tube. A certain amount of anticoagulant is injected into the sample tube according to the volume of hemolymph to mix with the hemolymph. Then, insert the tube stopper into the opening of the sample tube and repeatedly invert and mix several times to complete the entire hemolymph collection process. Attached Figure Description

[0015] Figure 1 This is a side view of the main body of a hemolymph collector for small shrimp according to the present invention.

[0016] Figure 2 This is a schematic diagram of the mixed sample collection structure of a hemolymph collector for small shrimp according to the present invention;

[0017] Figure 3 This is a schematic diagram of a collection needle structure for a hemolymph collector for small shrimp according to the present invention.

[0018] Figure 4 This is a schematic diagram of another collection needle structure for a hemolymph collector for small shrimp according to the present invention.

[0019] In the diagram: 1. End cap; 11. Inlet; 2. Collection tube retractor; 3. Collector body; 31. Sampling tube; 4. Tail retractor; 5. Collection needle; 51. Needle No. 1; 52. Needle No. 2; 6. Syringe; 61. Connector; 7. Anticoagulant; 8. Sample tube; 81. Tube opening; 82. Tube plug. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Example 1, please refer to Figure 1 , Figure 2 and Figure 3 A hemolymph collector for small shrimp includes a plug head 1, a collector body 3, and a collection needle 5. One end of the plug head 1 is connected to a collection tube constriction port 2, and the collector body 3 is connected to the end of the collection tube constriction port 2 away from the plug head 1. The collector body 3 is composed of multiple sets of sampling tubes 31. The end of the collector body 3 away from the collection tube constriction port 2 is connected to a tail end constriction port 4, and the top ends of the multiple sets of sampling tubes 31 are constricted at the collection tube constriction port 2, and the tail ends of the multiple sets of sampling tubes 31 are constricted at the tail end constriction port 4. The collection needle 5 is connected to the end of the tail end constriction port 4 away from the sampling tubes 31. The top of the plug head 1 is provided with a liquid inlet 11, and a syringe 6 is provided above the liquid inlet 11. A sample tube 8 is provided below the collection needle 5.

[0022] The plunger 1 is connected to the syringe 6 through the inlet 11, and the plunger 1 is connected to multiple sampling tubes 31 inside the collector body 3 through the collection tube constriction port 31. The collector body 3 is used to temporarily store the collected hemolymph of small shrimp, and the hemolymph is stored in multiple sampling tubes 31 inside the collector body 1. The two ends of the multiple sampling tubes 31 are constricted through the collection tube constriction port 31 and the tail end constriction port 4, respectively, so that the collector body 3 has a large capacity while making the volume of both ends smaller, making it easier to connect other components and penetrate the shrimp's body. The sample tube 8 is used to collect and store the shrimp hemolymph mixed with anticoagulant 7.

[0023] A connector 61 is provided at one end of the syringe 6 near the plunger 1, and the connector 61 and the plunger 1 are interlocked. A tube opening 81 is provided at the top of the sample tube 8, and a tube plug 82 is provided at the top of the tube opening 81. An appropriate amount of anticoagulant 7 is pre-stored inside the syringe 6. The inner diameter of the sampling tube 31 is 0.75-2.0 mm, and the length of the sampling tube 31 is 2-10 cm. The collector body 3 is composed of 3-6 sampling tubes 31, arranged side-by-side. The collection needle 5 and the multiple sampling tubes 31 within the collector body 3 are a single-piece structure. The collection needle 5 and the sampling tubes 31 are made of common materials suitable for capillary production, such as glass. The plunger 1 is made of plastic or... Made of stainless steel and other materials, the collection needle 5 and the sampling tube 31 form a 135-degree obtuse angle. The sample tube 8 is connected to the collector through the opening of the tube 81, and the collector injects shrimp hemolymph into the sample tube 8 through the tube 81. The tube plug 82 is used to seal the sample tube 8 to prevent the shrimp hemolymph from flowing out. The combination structure and size of the collection needle 5 and the main body of the collector 3 can reduce the wound caused by the collection of shrimp hemolymph and reduce the amount of hemolymph loss, thereby achieving effective collection. The connector 61 at the lower end of the syringe is tightly connected to the inlet 11 on the plug head 1 to prevent leakage when injecting anticoagulant 7 into the collector.

[0024] One configuration of the collection needle 5 involves multiple sampling tubes 31 whose tail ends converge into a single No. 1 needle 51. The No. 1 needle 51 and the multiple sampling tubes 31 are interconnected. By inserting the No. 1 needle 51 into the junction of the last pair of walking legs on the ventral side of the shrimp and the abdomen, hemolymph flows along the No. 1 needle 51, through the tail-end convergence port 4, and into the multiple sampling tubes 31. After collection, the injection needle 61 of the syringe 6 pre-loaded with anticoagulant 7 is inserted into the inlet 11 on the collector plug 1. Then, the first needle 51 of the sampler is inserted into the tube opening 81 of the sample tube 8. By pushing the piston of the syringe 6, the anticoagulant 7 is injected into the sampler. The anticoagulant 7 flows into the plug head 1 through the inlet 11 and enters the main body 3 of the sampler through the collection tube constriction port 2. The anticoagulant 7 is used to squeeze the hemolymph in each sampling tube 31 into the sample tube 8. Then, the tube plug 82 is inserted into the tube opening 81 of the sample tube 8. The entire hemolymph collection process is completed by repeatedly inverting and mixing several times.

[0025] Example 2, please refer to Figure 4 One type of structure of the collection needle 5 is that each of the tail ends of multiple sets of sampling tubes 31 is equipped with an independent No. 2 needle 52, and the multiple sets of No. 2 needles 52 are arranged side by side to form the collection needle 5. By inserting the No. 2 needle 52 into the connection between the base of the last pair of walking legs on the ventral side of the shrimp and the abdomen, the hemolymph from the multiple sets of No. 2 needles 52 enters into each sampling tube 31 respectively.

[0026] Working principle: When using the No. 1 needle 51 for insertion, the No. 1 needle 51 is inserted into the junction of the base of the last pair of walking legs on the ventral side of the shrimp and the abdomen. Hemolymph is then directed along the No. 1 needle 51, through the tail end constriction port 4, into multiple sampling tubes 31. After collection, the lower connector 61 of the syringe 6 pre-loaded with anticoagulant 7 is inserted into the inlet 11 on the collector plug head 1. Then, the No. 1 needle 51 of the collector is placed into the tube opening 81 on the sample tube 8. The syringe 6 is then pushed... The piston injects anticoagulant 7 into the sampler. Anticoagulant 7 flows into the plug head 1 through the inlet 11 and enters the main body 3 of the sampler through the collection tube constriction port 2. The anticoagulant 7 is used to squeeze the hemolymph in each sampling tube 31 and inject it into the sample tube 8 through the first needle. At the same time, a certain amount of anticoagulant 7 is injected into the sample tube 8 according to the volume of hemolymph to mix with the hemolymph. Then, the tube plug 82 is inserted into the tube opening 81 of the sample tube 8. The process is repeated several times to complete the entire hemolymph collection process.

[0027] When using a No. 2 needle with a 52-structure insertion;

[0028] By inserting the No. 2 needle 52 into the junction of the last pair of walking legs on the ventral side of the shrimp and the abdomen, hemolymph is drawn from multiple sets of No. 2 needles 52 into each sampling tube 31. After collection, the lower connector 61 of the syringe 6 pre-loaded with anticoagulant 7 is inserted into the inlet 11 on the collector plug head 1. Then, the No. 1 needle 51 of the collector is placed into the tube opening 81 on the sample tube 8. By pushing the piston of the syringe 6, the anticoagulant 7 is injected into the sampler. The anticoagulant 7 flows into the plug head 1 through the inlet 11 and enters the collector body 3 through the collection tube constriction port 2. The anticoagulant 7 is used to squeeze the hemolymph in each sampling tube 31 and inject it into the sample tube 8 through the No. 2 needle 52. At the same time, a certain amount of anticoagulant 7 is injected into the sample tube 8 according to the volume of hemolymph to mix with it. Then, the tube plug 82 is inserted into the tube opening 81 of the sample tube 8. The process is repeated several times to complete the entire hemolymph collection process.

Claims

1. A hemolymph collector for small shrimp, comprising a plug (1), a collector body (3), and a collection needle (5), characterized in that: One end of the plug (1) is connected to the collection tube converging port (2), and the collector body (3) is connected to the end of the collection tube converging port (2) away from the plug (1). The collector body (3) is composed of multiple sets of sampling tubes (31). The end of the collector body (3) away from the collection tube converging port (2) is connected to the tail end converging port (4). The top ends of the multiple sets of sampling tubes (31) are converging at the collection tube converging port (2), and the tail ends of the multiple sets of sampling tubes (31) are converging at the tail end converging port (4). The collection needle (5) is connected to the end of the tail end converging port (4) away from the sampling tube (31). The top of the plug (1) is provided with a liquid inlet (11), which is connected to the collector body (3) and the collection needle (5). The inner diameter of the sampling tube (31) is 0.75-2.0mm, and the length of the sampling tube (31) is 2-10cm. The main body (3) of the collector is composed of 3-6 sampling tubes (31), and multiple sampling tubes (31) are arranged in parallel. The sampling needle (5) and the multiple sampling tubes (31) in the main body (3) of the collector are integrally formed. The sampling needle (5) and the sampling tube (31) form an obtuse angle of 135 degrees.

2. The hemolymph collector for small shrimp according to claim 1, characterized in that: The plunger (1) is connected to the syringe (6) through the inlet (11), and the syringe (6) contains an anticoagulant (7).

3. The hemolymph collector for small shrimp according to claim 1, characterized in that: The collection needle (5) and sampling tube (31) are made of glass and common materials used in capillary production, and the plug (1) is made of plastic or stainless steel.

4. A hemolymph collector for small shrimp according to claim 2, characterized in that: The inlet (11) on the plunger (1) is fitted into the connector (61) on the syringe (6).

5. A hemolymph collector for small shrimp according to claim 4, characterized in that: One structure of the sampling needle (5) is that the tail end of multiple sampling tubes (31) is converged into a No. 1 needle (51), and the No. 1 needle (51) and the multiple sampling tubes (31) are connected.

6. A hemolymph collector for small shrimp according to claim 5, characterized in that: One structure of the collection needle (5) is that each of the tail ends of multiple sampling tubes (31) is provided with an independent second needle (52), and the multiple sets of second needles (52) are arranged side by side to form the collection needle (5).

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