A plasmid automatic extraction device

Abstract

The application discloses a kind of plasmid automatic extraction device, and its technical solution main points are including rotating centrifugal seat, the rotating centrifugal seat is set as the disc of vertical axis, the rotating centrifugal seat revolves around its own axis;Lysis tube;First filter screen, the position of each lysis tube inside near top is provided with first filter screen;Reagent adding mechanism, the reagent adding mechanism is used to add different reagents into lysis tube;Blocking plug;Purification pipe, the bottom of each lysis tube is provided with purification pipe;Second filter screen, the purification pipe is provided with second filter screen, and the second filter screen is used to filter after lysis bacterial liquid to obtain supernatant;GF membrane, the GF membrane is used to adsorb plasmid;And balancing liquid adding mechanism;Through the setting of rotating centrifugal seat, reagent adding mechanism and purification pipe, operator does not need to repeatedly operate, and the operation of plasmid extraction is simplified.

Description

Technical Field

[0001] This invention relates to the technical field of plasmid extraction, and more specifically, to an automated plasmid extraction device. Background Technology

[0002] Plasmids are crucial vectors in genetic engineering. Currently, plasmid extraction methods can be broadly categorized into two main steps: lysis and purification. The lysis step can be further divided into four sub-steps. The first sub-step involves pre-treating the bacterial culture to remove impurities. The second sub-step involves adding reagent #1 (50mM glucose / 25mM Tris-HCl / 10mM EDTA, pH 8.0). The third sub-step involves adding reagent #2 (0.2N NaOH / 1% SDS). The fourth sub-step involves adding reagent #3 (3M potassium acetate / 2M acetic acid). After completing these four sub-steps, the supernatant is obtained through filtration.

[0003] The second purification step involves adsorbing the plasmid supernatant obtained from the first step of lysis using a GF membrane, followed by two washes and one elution to obtain the plasmid DNA.

[0004] The above process requires at least 7 reagents and a variety of instruments. The operation involves the repeated use of centrifuge tubes, pipettes, and centrifuges, making it very cumbersome. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide an automated plasmid extraction device that simplifies plasmid extraction by eliminating the need for repeated operations by the operator through the design of a rotating centrifuge seat, a reagent addition mechanism, and a purification tube.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an automated plasmid extraction device, comprising a rotating centrifuge seat, wherein the rotating centrifuge seat is configured as a disk with a vertical axis, and the rotating centrifuge seat rotates around its own axis; The pyrolysis tubes are arranged on a rotating centrifuge base, and there are multiple pyrolysis tubes that are evenly arranged around the axis of the rotating centrifuge base. The first filter screen is installed near the top of each lysis tube. The first filter screen is used to filter out impurities in the bacterial solution. A reagent adding mechanism is used to add different reagents into the pyrolysis tube; The sealing plug is provided with an open top and bottom for the pyrolysis tube. The sealing plug is used to seal the bottom of the pyrolysis tube. The sealing plug can be opened and closed by sliding up and down. Purification tubes are provided at the bottom of each lysis tube; The purification tube is equipped with a second filter screen, which is used to filter the lysed bacterial solution to obtain the supernatant. A GF membrane, wherein the GF membrane is used to adsorb plasmids; And a equilibration solution addition mechanism, which is used to add equilibration solution into the purification tube.

[0007] The present invention is further configured such that: a switching mechanism is provided on the rotating centrifuge seat, the switching mechanism being used to drive the sealing plug to move up and down; The switching mechanism includes a connecting rod, which is vertically installed inside the pyrolysis tube. One bottom end of the connecting rod is fixedly connected to the sealing plug, and the top end extends out from the top of the pyrolysis tube. The connecting rod is attached to the inner wall of the pyrolysis tube. A lifting rod is provided on top of the rotating centrifugal seat and can slide up and down. The top end of the lifting rod is connected to the top end of the connecting rod. An electromagnet is fixedly connected to the top of the rotating centrifuge base. The electromagnet has a vertically arranged guide hole, and the bottom of the lifting rod is inserted into the guide hole. A magnetic attractor, which can be attracted by an electromagnet when energized, and is fixedly connected to the outside of the lifting rod; And an elastic element, which is disposed between the electromagnet and the magnetic attracting element, wherein the elastic element is in a compressed state to apply an upward elastic force to the magnetic attracting element.

[0008] The present invention is further configured such that: the first filter screen is a cylindrical shape with an open top, and a groove is provided on one side of the first filter screen that is recessed toward the axis of the first filter screen, and the connecting rod passes through the groove; Multiple suspension blocks are fixedly connected to the top of the first filter screen, and the suspension blocks are suspended from the top of the pyrolysis tube.

[0009] The present invention is further configured such that: the reagent adding mechanism includes multiple adding components, each adding component corresponding to a lysis tube; The addition component includes an addition tube, the axis of which is set horizontally, the height of which is higher than the height of the pyrolysis tube, the end of which is closed near the pyrolysis tube, and the end of which is open away from the pyrolysis tube. A feeding pipe, one end of which is fixedly connected to the end of the adding pipe near the pyrolysis pipe, and the other end is located above the pyrolysis pipe, and the feeding pipe is connected to the pyrolysis pipe; An adding rod is provided, one end of which extends into the adding tube from the end away from the pyrolysis tube, and a piston is provided at the end of the adding rod that extends into the pyrolysis tube; And a cylinder, wherein the piston rod of the cylinder is fixedly connected to the adding rod, and the cylinder pushes the adding rod to slide through the piston rod.

[0010] The present invention is further configured such that: the reagent adding mechanism also includes a storage tank, there are multiple storage tanks, each storage tank corresponds to a reagent, each storage tank is connected to each adding pipe through a pipe, and the storage tank sends the reagent into each feeding pipe through a water pump.

[0011] The present invention is further configured such that: the adding component also includes a support plate, and the adding pipe and the cylinder are all fixedly connected to the support plate; A stop plate is provided, which is mounted on a support plate at the end of the adding tube away from the pyrolysis tube. Multiple stop plates are arranged along the axis of the adding tube. The stop plates can move up and down on the support plate. Multiple cylinders corresponding to the stop plates are fixedly connected to the bottom of the support plate. The stop plates are driven to move up and down by the corresponding cylinders. When the stop plate rises, it can block the end of the adding rod away from the pyrolysis tube to prevent the adding rod from sliding in the direction away from the pyrolysis tube. Each stop plate corresponds to at least one reagent, and the amount of reagent added is the same for the same stop plate. When the stop plate contacts the adding rod, the amount of reagent that needs to be added between the piston and the end of the adding tube near the pyrolysis tube is the amount of reagent corresponding to the stop plate.

[0012] The present invention is further configured such that: a vacuum tube is provided at the bottom of the purification tube, and a vacuum chamber is provided at the bottom of the multiple vacuum tubes, the vacuum chamber being connected to a vacuum pumping device.

[0013] The present invention is further configured such that: a translation plate is fixedly connected to the bottom of the purification tube, and the vacuum tube is fixedly connected to the translation plate and penetrates the translation plate; The translation plate is equipped with plasmid test tubes, and the plasmid test tubes and vacuum tubes are arranged along the horizontal sliding direction of the translation plate. The portion of the vacuum tube located below the translation plate is configured as a flexible tube. The translation plate is driven by a cylinder to slide horizontally.

[0014] The present invention is further configured such that: the equilibrium liquid addition mechanism includes multiple addition components, each addition component corresponds to a purification tube, and the end of the feeding tube in the addition component that is away from the addition tube is located above the corresponding purification tube; And a storage tank containing a balancing fluid, which is supplied to the addition tube of the addition component via a pipeline.

[0015] The present invention is further configured to include a protective housing, wherein the storage tank and the storage container are disposed outside the protective housing.

[0016] In summary, the present invention has the following advantages over the prior art: the present invention simplifies the plasmid extraction process by eliminating the need for repeated operations by the operator through the design of the rotating centrifuge seat, reagent addition mechanism, and purification tube. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the embodiment; Figure 2 This is a schematic diagram showing the removal of the protective casing in an embodiment. Figure 3 for Figure 2 Enlarged schematic diagram of part A; Figure 4 for Figure 2 Enlarged schematic diagram of part B; Figure 5 This is a cross-sectional view illustrating the switching mechanism in an embodiment; Figure 6 A cross-sectional view of the purification tube used in this embodiment; Figure 7 This is a schematic diagram illustrating the groove in an embodiment.

[0018] In the diagram: 1. Protective shell; 11. Storage tank; 12. Storage tank; 2. Rotary centrifuge seat; 3. Pyrolysis tube; 31. First filter screen; 311. Groove; 312. Suspension block; 32. Sealing plug; 4. Switching mechanism; 41. Connecting rod; 42. Lifting rod; 43. Electromagnet; 431. Guide hole; 44. Magnetic suction element; 45. Elastic element; 5. Addition component; 51. Support plate; 52. Addition tube; 53. Feeding tube; 54. Addition rod; 55. Stop plate; 6. Purification tube; 61. Second filter screen; 62. GF membrane; 7. Translation plate; 71. Plasmid test tube; 8. Vacuum chamber; 81. Vacuum tube. Detailed Implementation

[0019] To enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Based on the embodiments in this application, other similar embodiments obtained by those skilled in the art without creative effort should all fall within the scope of protection of this application. Furthermore, directional terms mentioned in the following embodiments, such as "up," "down," "left," and "right," are only for reference to the directions in the accompanying drawings; therefore, the directional terms used are for illustrative purposes and not for limiting the invention.

[0020] The present invention will be further described below with reference to the accompanying drawings and preferred embodiments.

[0021] Example: An automated plasmid extraction device, see attached document. Figure 1 - Appendix Figure 7The system includes a protective housing 1, a rotating centrifuge seat 2 housed within the protective housing 1, multiple lysis tubes 3 mounted on the rotating centrifuge seat 2, a first filter screen 31 within each lysis tube 3, a reagent adding mechanism for adding reagents to each lysis tube 3, a sealing plug 32 for sealing the bottom of each lysis tube 3, a purification tube 6 located below each lysis tube 3, a second filter screen 61 within each purification tube 6, a GF membrane 62 located within each purification tube 6 and below the second filter screen 61, and a equilibration solution adding mechanism for adding equilibration solution to each purification tube 6. The rotating centrifuge seat 2 is a vertically oriented disc that rotates around its own axis. Multiple lysis tubes 3 are evenly arranged around the axis of the rotating centrifuge seat 2. The top and bottom of each lysis tube 3 are open, and the sealing plug 32 opens and closes the bottom of the lysis tube 3 by sliding up and down. The second filter screen 61 filters the lysed bacterial solution to obtain supernatant, and the GF membrane 62 adsorbs plasmids. Specifically, the lysis tubes 3 and the purification tubes 6 are both housed within the protective housing 1.

[0022] Specifically, bacterial suspension is added to each lysis tube 3. After the bacterial suspension is injected into the lysis tube 3, the first filter screen 31 in the lysis tube 3 filters out impurities in the bacterial suspension, and the filtered bacterial suspension falls to the bottom of the lysis tube 3. Then, reagent No. 1 (50mM glucose / 25mM Tris-HCl / 10mM EDTA, pH 8.0), reagent No. 2 (0.2N NaOH / 1% SDS), and reagent No. 3 (3M potassium acetate / 2M acetic acid) are added sequentially through the reagent addition mechanism to perform operations such as suspending bacterial cells, lysing bacterial cells, and precipitating proteins and genomic DNA in the bacterial suspension, respectively. After each addition of reagents, the bacterial suspension and reagents can be mixed by centrifuging the centrifuge seat 2. Then, equilibration solution is added to the purification tube 6 through the equilibration solution addition mechanism to wash the GF membrane 62. After that, the sealing plug 32 is slid down to allow the bacterial suspension to fall into the purification tube 6 and pass through the second filter screen 61. During the process, the bacterial culture is filtered to obtain a supernatant. As the supernatant flows downward through the GF membrane 62, the plasmid is adsorbed onto the GF membrane 62. Then, washing buffer is added twice to the lysis tube 3 through the reagent addition mechanism. Both washing buffers enter the purification tube 6 through the lysis tube 3 to wash the plasmid. Then, elution buffer is added to the lysis tube 3 through the reagent addition mechanism. The elution buffer enters the purification tube 6 through the lysis tube 3 to elute the plasmid on the GF membrane 62, and the eluted plasmid is stored in the elution buffer.

[0023] Specifically, the protective housing 1 is provided with multiple doors that can be opened and closed (not shown in the figure) to facilitate the addition of bacterial solution and the removal of elution solution containing plasmids.

[0024] Specifically, a motor is installed on the top of the rotating centrifuge seat 2. The motor is fixedly connected to the protective housing 1 and is connected to the rotating centrifuge seat 2 to drive the rotating centrifuge seat 2 to rotate.

[0025] Specifically, a switching mechanism 4 is provided on the rotating centrifuge seat 2, which is used to drive the sealing plug 32 to move up and down. The switching mechanism 4 includes a connecting rod 41, a lifting rod 42, an electromagnet 43, a magnetic attractor 44, and an elastic element 45. The connecting rod 41 is vertically arranged inside the pyrolysis tube 3. One bottom end of the connecting rod 41 is fixedly connected to the sealing plug 32, and the top end extends from the top of the pyrolysis tube 3. The connecting rod 41 is attached to the inner wall of the pyrolysis tube 3. The lifting rod 42 is arranged on the top of the rotating centrifuge seat 2 and can slide up and down. One top end of the lifting rod 42 is connected to one top end of the connecting rod 41. The electromagnet 43 is fixedly connected to the top of the rotating centrifuge seat 2. The electromagnet 43 is provided with a vertically arranged guide hole 431. The bottom of the lifting rod 42 is inserted into the guide hole 431. The magnetic attractor 44 can be attracted by the electromagnet 43 when it is energized. The magnetic attractor 44 is fixedly connected to the outside of the lifting rod 42. An elastic element 45 is disposed between the electromagnet 43 and the magnetic attractor 44. When the elastic element 45 is in a compressed state, it applies an upward elastic force to the magnetic attractor 44.

[0026] When the electromagnet 43 is not energized, under the action of the elastic element 45, the lifting rod 42 moves upward and drives the connecting rod 41 to move upward, so that the connecting rod 41 can drive the sealing plug 32 to press upward and press against the bottom of the pyrolysis tube 3 to seal the bottom of the pyrolysis tube 3; when it is necessary to open the bottom of the pyrolysis tube 3, the electromagnet 43 is energized, and under the attraction of the electromagnet 43, the magnetic suction element 44 moves downward, thereby moving downward through the lifting rod 42 and the connecting rod 41, thereby driving the sealing plug downward to disengage from the bottom of the pyrolysis tube 3, so that the bottom of the pyrolysis tube 3 is opened.

[0027] Specifically, the elastic element 45 is configured as a spring, which is elastically sleeved outside the connecting rod 41 and its two ends are in contact with the magnetic element 44 and the electromagnet 43, respectively.

[0028] Specifically, the first filter screen 31 is configured as a cylindrical shape with an open top. A groove 311 is provided on one side of the first filter screen 31, recessed towards its axis, through which the connecting rod 41 passes. The groove 311 ensures that the first filter screen 31 does not obstruct the vertical movement of the connecting rod 41. Multiple suspension blocks 312 are fixedly connected to the top of the first filter screen 31, and these suspension blocks 312 are suspended from the top of the pyrolysis tube 3.

[0029] Specifically, the reagent addition mechanism includes multiple addition components 5, each corresponding to a pyrolysis tube 3. Each addition component 5 includes an addition tube 52, a feeding tube 53, an addition rod 54, and a cylinder. The axis of the addition tube 52 is horizontally positioned, and its height is higher than that of the pyrolysis tube 3. The end of the addition tube 52 near the pyrolysis tube 3 is closed, while the end away from the pyrolysis tube 3 is open. One end of the feeding tube 53 is fixedly connected to the end of the addition tube 52 near the pyrolysis tube 3, and the other end is positioned above the pyrolysis tube 3, communicating with it. One end of the addition rod 54 extends from the end of the addition tube 52 away from the pyrolysis tube 3 into the addition tube 52, and a piston is provided at the end of the addition rod 54 extending into the pyrolysis tube 3. The piston rod of the cylinder is fixedly connected to the addition rod 54, and the cylinder pushes the addition rod 54 to slide via the piston rod.

[0030] When adding reagents, the reagents are first introduced into the addition tube 52, and then the piston rod drives the piston to slide towards the direction of the pyrolysis tube 3. Thus, the reagents in the addition tube 52 are sent into the pyrolysis tube 3 through the feeding tube 53 by the compression of the piston.

[0031] Specifically, the reagent adding mechanism also includes storage tanks 11, of which multiple storage tanks 11 are provided. Each storage tank 11 stores a corresponding reagent. Each storage tank 11 is connected to each adding pipe 52 through a pipe. The storage tank 11 sends the reagent into each feeding pipe 53 through a water pump.

[0032] Specifically, the addition component 5 also includes a support plate 51 and a stop plate 55. The addition tube 52 and the cylinder are fixedly connected to the support plate 51. The stop plate 55 is set on the support plate 51 and located at the end of the addition tube 52 away from the pyrolysis tube 3. There are multiple stop plates 55, which are arranged along the axial direction of the addition tube 52. The stop plates 55 can move up and down on the support plate 51. The bottom of the support plate 51 is fixedly connected to multiple cylinders corresponding to the multiple stop plates 55. The stop plates 55 are driven to move up and down by the corresponding cylinders. When the stop plate 55 rises, it can block the end of the addition rod 54 away from the pyrolysis tube 3 to prevent the addition rod 54 from sliding in the direction away from the pyrolysis tube 3. Each stop plate 55 corresponds to at least one reagent. The amount of reagent added corresponding to the same stop plate 55 is the same. When the stop plate 55 contacts the addition rod 54, the amount of reagent contained between the piston and the end of the addition tube 52 near the pyrolysis tube 3 is the amount of reagent that needs to be added corresponding to the stop plate 55.

[0033] When it is necessary to add reagent to the lysis tube 3, the storage tank 11 containing the corresponding reagent injects the reagent into the addition tube 52. At this time, the stop plate 55 corresponding to the reagent moves upward to block the addition rod 54. As the reagent is injected into the addition tube 52, the addition rod 54 moves away from the lysis tube 3 until it is pressed against the stop plate 55. At this time, the amount of reagent stored in the addition tube 52 is the amount of reagent that needs to be added. By setting the stop plate 55, the amount of different reagents can be controlled, improving the accuracy of reagent addition.

[0034] Specifically, a vacuum tube 81 is provided at the bottom of the purification tube 6, and a vacuum chamber 8 is provided at the bottom of multiple vacuum tubes 81. The vacuum chamber 8 is connected to a vacuum pumping device. A translation plate 7 is fixedly connected to the bottom of the purification tube 6, and the vacuum tubes 81 are fixedly connected to and pass through the translation plate 7. Plasmid test tubes 71 are provided on the translation plate 7. The plasmid test tubes 71 and the vacuum tubes 81 are arranged along the horizontal sliding direction of the translation plate 7. The portion of the vacuum tube 81 at least below the translation plate 7 is a flexible tube. The translation plate 7 is driven by a cylinder to slide horizontally.

[0035] The movement of the translation plate 7 allows adjustment of whether the purification tube 6 is aligned with the vacuum tube 81 or the plasmid tube 71. When bacterial culture and washing solution are injected into the purification tube 6, the purification tube 6 is aligned with the vacuum tube 81 to allow the filtered bacterial culture and washing solution to be extracted from the purification tube 6. When eluent is added to the purification tube 6, the plasmid tube 71 is aligned with the purification tube 6 to allow the eluent containing plasmids to enter the plasmid tube 71 and accommodate the extracted plasmids.

[0036] Specifically, the equilibrium solution addition mechanism includes multiple addition components 5 and a storage tank 12. Each addition component 5 corresponds to a purification tube 6. The end of the feed tube 53 in the addition component 5 that is away from the addition tube 52 is located above the corresponding purification tube 6. The storage tank 12 stores equilibrium solution, and the storage tank 12 delivers equilibrium solution to the addition tube 52 of the addition component 5 through a pipeline.

[0037] The above description is merely a preferred embodiment of the present invention. The scope of protection of the present invention is not limited to the above embodiments. All technical solutions falling within the scope of the present invention's concept are within the scope of protection of the present invention. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principles of the present invention should also be considered within the scope of protection of the present invention.

Claims

1. An automated plasmid extraction device, characterized in that: Includes a rotating centrifuge seat (2), which is configured as a disk with a vertical axis, and the rotating centrifuge seat (2) rotates around its own axis; The pyrolysis tube (3) is set on the rotating centrifuge seat (2), and multiple pyrolysis tubes (3) are evenly arranged around the axis of the rotating centrifuge seat (2); First filter screen (31): A first filter screen (31) is provided near the top of each lysis tube (3). The first filter screen (31) is used to filter out impurities in the bacterial solution. A reagent adding mechanism is used to add different reagents into the pyrolysis tube (3); The top and bottom of the pyrolysis tube (3) are open. The sealing plug (32) is used to seal the bottom of the pyrolysis tube (3). The sealing plug (32) can open and close the bottom of the pyrolysis tube (3) by sliding up and down. Purification tube (6), each lysis tube (3) has a purification tube (6) at the bottom; The second filter (61) is provided inside the purification tube (6). The second filter (61) is used to filter the lysed bacterial solution to obtain the supernatant. A GF membrane (62) is used to adsorb plasmids; And a balancing solution adding mechanism, which is used to add balancing solution into the purification tube (6); The reagent addition mechanism includes multiple addition components (5), each addition component (5) corresponding to a pyrolysis tube (3); The addition component (5) includes an addition tube (52), the axis of which is set horizontally, the height of which is higher than the height of the pyrolysis tube (3), the end of which is closed near the pyrolysis tube (3), and the end of which is open away from the pyrolysis tube (3).

2. The automated plasmid extraction device according to claim 1, characterized in that: The rotating centrifugal seat (2) is provided with a switching mechanism (4), which is used to drive the sealing plug (32) to move up and down; The switching mechanism (4) includes a connecting rod (41), which is vertically installed inside the pyrolysis tube (3). One bottom end of the connecting rod (41) is fixedly connected to the sealing plug (32), and the top end extends out from the top of the pyrolysis tube (3). The connecting rod (41) is attached to the inner wall of the pyrolysis tube (3). Lifting rod (42), the lifting rod (42) is set on the top of the rotating centrifugal seat (2) and can slide up and down, the top end of the lifting rod (42) is connected to the top end of the connecting rod (41); An electromagnet (43) is fixedly connected to the top of the rotating centrifugal seat (2). The electromagnet (43) is provided with a vertically arranged guide hole (431). The bottom of the lifting rod (42) is inserted into the guide hole (431). A magnetic attractor (44) is attached to the outside of the lifting rod (42) when the electromagnet (43) is energized. And an elastic element (45) is disposed between the electromagnet (43) and the magnetic attractor (44), wherein the elastic element (45) is in a compressed state to apply an upward elastic force to the magnetic attractor (44).

3. The automated plasmid extraction device according to claim 2, characterized in that: The first filter screen (31) is configured as a cylindrical shape with an open top. A groove (311) is provided on one side of the first filter screen (31) that is recessed towards the axis of the first filter screen (31). The connecting rod (41) passes through the groove (311). The top of the first filter screen (31) is fixedly connected with a plurality of suspension blocks (312), which are suspended on the top of the pyrolysis tube (3).

4. The automated plasmid extraction device according to claim 3, characterized in that: The reagent addition mechanism also includes a storage tank (11), and there are multiple storage tanks (11). Each storage tank (11) stores a corresponding reagent. Each storage tank (11) is connected to each addition tube (52) through a pipe. The storage tank (11) sends the reagent into each feeding tube (53) through a water pump.

5. The automated plasmid extraction device according to claim 4, characterized in that: The addition component (5) also includes a support plate (51), and the addition tube (52) and cylinder are fixedly connected to the support plate (51); A stop plate (55) is provided on a support plate (51) and located at the end of the adding pipe (52) away from the pyrolysis pipe (3). Multiple stop plates (55) are provided and arranged along the axis of the adding pipe (52). The stop plates (55) can move up and down on the support plate (51). Multiple cylinders corresponding to the multiple stop plates (55) are fixedly connected to the bottom of the support plate (51). The stop plates (55) are driven to move up and down by the corresponding cylinders. The plate (55) rises upward to block the end of the adding rod (54) away from the pyrolysis tube (3) to prevent the adding rod (54) from sliding away from the pyrolysis tube (3). Each stop plate (55) corresponds to at least one reagent. The amount of reagent added corresponding to the same stop plate (55) is the same. When the stop plate (55) contacts the adding rod (54), the amount of reagent contained between the piston and the end of the adding tube (52) near the pyrolysis tube (3) is the amount of reagent that needs to be added corresponding to the stop plate (55).

6. The automated plasmid extraction device according to claim 5, characterized in that: The purification tube (6) is provided with a vacuum tube (81) at the bottom, and a vacuum chamber (8) is provided at the bottom of the multiple vacuum tubes (81). The vacuum chamber (8) is connected to a vacuum pumping device.

7. The automated plasmid extraction device according to claim 6, characterized in that: The bottom of the purification tube (6) is fixedly connected to a translation plate (7), and the vacuum tube (81) is fixedly connected to the translation plate (7) and passes through the translation plate (7); The translation plate (7) is provided with plasmid test tubes (71), and the plasmid test tubes (71) and vacuum tubes (81) are arranged along the horizontal sliding direction of the translation plate (7). The portion of the vacuum tube (81) located at least below the translation plate (7) is configured as a flexible tube. The translation plate (7) is driven by a cylinder to slide horizontally.

8. The automated plasmid extraction device according to claim 7, characterized in that: The equilibrium liquid addition mechanism includes multiple addition components (5), each addition component (5) corresponds to a purification tube (6), and the end of the feeding tube (53) in the addition component (5) away from the addition tube (52) is located above the corresponding purification tube (6); And a storage tank (12) containing a balancing liquid, which supplies the balancing liquid to the addition pipe (52) of the addition component (5) via a pipeline.

9. The automated plasmid extraction device according to claim 8, characterized in that: It also includes a protective housing (1), and the storage tank (11) and storage tank (12) are disposed outside the protective housing (1).

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