A method for extracting dry flower extract, dry flower extract prepared and applications thereof

Abstract

The application discloses a kind of extraction methods of dry flower extract, including dry flower crushing, with eutectic solvent mixing, ultrasonic, constant temperature oscillation extraction, filtration is obtained.The application osmanthus dry flower and eutectic solvent use 1:15 of material liquid ratio, and add the water of dry flower mass 50%, and the amount of total phenol and total flavone in the osmanthus dry flower obtained by extraction is the best comprehensive effect.Constant temperature oscillation incubator uses 170r / min of frequency, 25 DEG C temperature, and the total phenol and total flavone content in the dry flower extract prepared is high, has excellent antioxidant, reducing effect, and also has certain bacteriostatic effect, can be used as natural preservative.Application in dry flower extraction, prolongs the service life of flower, expands the application range, and applied in cosmetics has anti-aging, whitening effect, and no irritation, suitable for sensitive skin use.

Description

Technical Field

[0001] This invention relates to a method for extracting dried flower extracts, the resulting dried flower extracts, and their applications, and relates to BO1D, specifically to the field of separation. Background Technology

[0002] Fresh flowers have a short lifespan, and relying solely on their blooming period cannot achieve widespread application. Dried flowers are a method developed to expand the utilization rate of fresh flowers and extend their lifespan. However, extracting from fresh flowers after drying significantly reduces the processing difficulty and extraction efficiency. Osmanthus (Osmanthus sp.) is a famous native Chinese flower with high ornamental value, and its edible and medicinal values ​​are increasingly being studied. However, Osmanthus has a short flowering period. Typically, Osmanthus flowers are freeze-dried or vacuum-dried for storage. The reduced moisture content after drying makes extraction difficult, posing a challenge for further processing and development. Therefore, developing a highly efficient extraction process for dried Osmanthus flowers is crucial.

[0003] Chinese invention patent CN201410043501.8 discloses an extract of osmanthus phenylethanoid glycosides, its preparation method, and its uses. By adding an acidic component beforehand and using an alcoholic solution for extraction, the oxidative deterioration of the osmanthus extract is reduced, and the extraction stability is improved. It can be widely used in various fields. However, while the extracted osmanthus phenylethanoid glycosides have an effect on improving diabetes, the extraction of other components from osmanthus is not addressed. Chinese invention patent CN200910273100.0 discloses an osmanthus extract, its preparation method, and its application in liver protection. The extract is obtained by organic solvent extraction, followed by vacuum concentration and purification with macroporous resin. The prepared extract has high purity and liver-protective effects; however, the use of organic solvent extraction raises certain environmental concerns. Summary of the Invention

[0004] To improve the extraction effect of dried flowers, reduce the extraction difficulty, and enable the dried flower extract to have antioxidant effects, the first aspect of the present invention provides a method for extracting dried flower extract, comprising the following steps:

[0005] (1) Crush the dried flowers, add them to a eutectic solvent, add water, and shake well;

[0006] (2) Then it is placed in an ultrasonic cell disruptor for ultrasonication;

[0007] (3) Then transfer it to a constant temperature shaking incubator for constant temperature reaction;

[0008] (4) Finally, filter the material and take the filtered material to obtain dried flower extract, and test the content of effective ingredients.

[0009] In a preferred embodiment, the raw materials for preparing the eutectic solvent include one or a combination of several of the following: ionizable substances, organic matter, and ionic liquids.

[0010] As a preferred embodiment, the method for preparing the eutectic solvent includes the following steps: adding an ionizable substance to an organic compound, heating in a water bath to 75-85°C, stirring continuously during the heating process until the mixture is homogeneous, and then cooling for later use.

[0011] In a preferred embodiment, the ionizable substance is an electron-donating substance, the organic substance is an electron-accepting substance, and the ionizable substance is selected from one or a combination of several of choline chloride, 2-hydroxypropionic acid, urea, citric acid, and ascorbic acid.

[0012] In a preferred embodiment, the raw materials for preparing the eutectic solvent are a combination of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol. The weight ratio of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol is 2:1:1:(5-10).

[0013] In a preferred embodiment, the weight ratio of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol is 2:1:1:6.

[0014] In a preferred embodiment, the weight ratio of the dried flowers to the eutectic solvent is 1:(5-50).

[0015] In a preferred embodiment, the weight ratio of the dried flowers to the eutectic solvent is 1:15.

[0016] In a preferred embodiment, the amount of water added in step 1 is 10-60% of the mass of the dried flowers.

[0017] In a preferred embodiment, the amount of water added in step 1 is 50% of the mass of the dried flowers.

[0018] In a preferred embodiment, the dried flowers in step 1 are dried osmanthus flowers.

[0019] The optimal extraction ratio of total phenols and flavonoids was 1:15 when dried osmanthus flowers were mixed with the eutectic solvent, followed by the addition of 50% (by weight of the dried osmanthus flowers) of water for further extraction. This resulted in the highest overall extraction yield of total phenols and flavonoids. A possible reason for this is that the osmanthus flowers used in this application were dried, resulting in higher surface tension and poor wetting of the eutectic solvent. This hindered the exchange of substances between the total flavonoids and the solvent, leading to lower extraction efficiency. In this application, a 1:15 ratio of dried osmanthus flowers to the eutectic solvent, combined with 50% (by weight of the dried flowers) of water, created a system with a polarity similar to that of the total phenols and flavonoids in the dried osmanthus flowers, resulting in the highest extraction yield of these substances. Furthermore, the ratio of dried osmanthus flowers to eutectic solvent is 1:15. Since the eutectic solvent has a certain viscosity, under the optimized ratio, the dried osmanthus flowers can be evenly dispersed in the eutectic solvent, reducing the problem of insufficient extraction caused by the overlapping of dried osmanthus flower fragments.

[0020] In a preferred embodiment, the reaction temperature in step 3 is 15-35°C, and the reaction time is 20-30 hours.

[0021] In a preferred embodiment, the reaction temperature in step 3 is 25°C, and the reaction time is 24 hours. Preferably, the oscillation frequency in step 3 is 100-190 r / min. More preferably, the oscillation frequency in step 3 is 170 r / min.

[0022] The extraction rate was high when the frequency of constant temperature shaking was 170 r / min and the temperature was 25℃. This may be because the dried osmanthus flowers need to undergo an exchange reaction with the eutectic solvent in the constant temperature shaking incubator. Under the preferred conditions, the total phenols and total flavonoids in the dried osmanthus flowers dissolved at a higher rate. However, if the extraction parameters are exceeded, the molecular mobility decreases and the exchange efficiency of substances weakens. If the vibration frequency is too high, the solvent cannot achieve sufficient wetting in a short time, thus affecting the dissolution of total phenols and total flavonoids.

[0023] In a preferred embodiment, the ultrasound time in step 2 is 0-25 min; more preferably, the ultrasound time in step 2 is 20 min.

[0024] In a preferred embodiment, the filtration method in step 4 is gauze filtration, using 7-10 layers of gauze.

[0025] A second aspect of the present invention provides a dried flower extract prepared by the aforementioned method for extracting dried flower extracts.

[0026] A third aspect of the present invention provides an application of dried flower extract in food, medicine, beverages, and cosmetics.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] (1) The extraction method of the dried flower extract of the present invention uses a material-liquid ratio of 1:15 between dried osmanthus flowers and a eutectic solvent, and adds water at 50% of the mass of the dried flowers. The combined effect of the total phenols and total flavonoids in the extracted dried osmanthus flowers is the best.

[0029] (2) The extraction method of the dried flower extract described in this invention uses a constant temperature shaking incubator with a frequency of 170 r / min and a temperature of 25°C. The dried flower extract prepared has a high content of total phenols and total flavonoids, has excellent antioxidant and reducing effects, and also has a certain antibacterial effect, and can be used as a natural preservative.

[0030] (3) The extraction method of dried flower extract described in this invention, when applied to dried flower extraction, prolongs the lifespan of the flowers, expands the scope of application, and when applied to cosmetics, has anti-aging and whitening effects, and is non-irritating and suitable for sensitive skin. Attached Figure Description

[0031] Figure 1 Images of the Escherichia coli inhibition zone test of the dried osmanthus flower extract in Example 1;

[0032] Figure 2 The image shows the Staphylococcus aureus inhibition zone test of the dried osmanthus flower extract in Example 1.

[0033] Figure 3 Image of the Bacillus subtilis inhibition zone test of Osmanthus fragrans dried flower extract in Example 1;

[0034] Figure 4 Image of the Bacillus cereus inhibition zone test of Osmanthus fragrans dried flower extract in Example 1;

[0035] Figure 5-7 The growth of Aspergillus niger after 24, 48, and 72 hours was measured using a 5 wt% blank eutectic solvent.

[0036] Figure 8-10 Example 1: Growth of Aspergillus niger after 24, 48, and 72 hours using 5% Osmanthus fragrans flower extract.

[0037] Figure 11-13 The growth of Aspergillus niger after 24, 48, and 72 hours was measured using a 10 wt% blank eutectic solvent.

[0038] Figure 14-16 The growth of Aspergillus niger after 24, 48, and 72 hours was observed using 10wt% Osmanthus fragrans flower extract from Example 1.

[0039] Figure 17Images of chicken embryos tested during the 0s stimulus in the control group;

[0040] Figure 18 Images of chicken embryos under 180s stimulation;

[0041] Figure 19 Image of chicken embryo during 0s stimulation in Example 1;

[0042] Figure 20 This is a picture of a chicken embryo under 180s stimulation, as shown in Example 1. Detailed Implementation

[0043] Example 1

[0044] A method for extracting dried flower extract, comprising the following steps:

[0045] (1) Crush the dried flowers, add them to a eutectic solvent, add water, and shake well;

[0046] (2) Then it is placed in an ultrasonic cell disruptor for ultrasonication;

[0047] (3) Then transfer it to a constant temperature shaking incubator for constant temperature reaction;

[0048] (4) Finally, filter the material and take the filtered material to obtain dried flower extract, and test the content of effective ingredients.

[0049] The raw materials for preparing the eutectic solvent are a combination of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol, with a total mass of 100g and a mass ratio of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol of 2:1:1:6.

[0050] The method for preparing the eutectic solvent includes the following steps: mixing citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol, heating in a water bath to 80°C, stirring continuously during heating until the mixture is homogeneous, and then cooling for later use.

[0051] The weight ratio of the dried flowers to the eutectic solvent is 1:15. In step 1, the amount of water added is 50% of the mass of the dried flowers. The dried flowers are dried osmanthus flowers, purchased from the Guilin City Agricultural Trade Market.

[0052] The reaction temperature in step 3 is 25℃, the reaction time is 24h, and the oscillation frequency is 170r / min.

[0053] The ultrasonic time in step 2 is 20 minutes. The filtration method in step 4 is gauze filtration, using 8 layers of gauze.

[0054] Example 2

[0055] A method for extracting dried flower extract, using the same raw materials as in Example 1, except that the weight ratio of the dried flowers to the eutectic solvent is 1:50. The preparation method is the same as in Example 1.

[0056] Example 3

[0057] A method for extracting dried flower extract, using the same raw materials as in Example 1, except that the amount of water added in step 1 is 10% of the mass of the dried flowers. The preparation method is the same as in Example 1.

[0058] Example 4

[0059] A method for extracting dried flower extracts, using the same raw materials as in Example 1, except that the reaction temperature in step 3 is 20°C. The preparation method is the same as in Example 1.

[0060] Example 5

[0061] A method for extracting dried flower extracts, using the same raw materials as in Example 1, except that the oscillation frequency in step 3 is 150 r / min. The preparation method is the same as in Example 1.

[0062] Example 6

[0063] A method for extracting dried flower extracts, using the same raw materials as in Example 1, except that the ultrasonic time in step 2 is 0 min. The preparation method is the same as in Example 1.

[0064] Performance testing

[0065] 1. Determination of total phenol content:

[0066] (1) Preparation of gallic acid standard curve: Gallic acid was prepared into a standard solution of 0.065 mg / mL. 0.00 mL, 0.20 mL, 0.40 mL, 0.60 mL, 0.80 mL, 1.00 mL and 1.20 mL of the above standard solution were accurately measured into 25.0 mL stoppered test tubes. 1.00 mL of Folin-Phenol colorimetric reagent was added and mixed well. Then 5.0 mL of 1 mol / L sodium carbonate solution was added and the solution was diluted to the mark with distilled water. The mixture was mixed well and placed at room temperature and in the dark for 1 h. The absorbance value at 760 nm was measured. The standard curve was plotted with the gallic acid concentration as the abscissa and the absorbance value as the ordinate.

[0067] (2) Determination of total phenols in the sample: Accurately transfer 1.00 mL of dried flower extract extracted by eutectic solvent in Examples 1-7 into a 25.0 mL test tube, and determine the absorbance value according to the method in (1) above. Find the corresponding value in the gallic acid standard curve based on the absorbance value. The total phenol content of the sample is expressed as the relative amount of gallic acid (g GAE / 100g).

[0068]

[0069] 2. Determination of total flavonoid content:

[0070] (1) Preparation of rutin standard curve: Prepare a 0.7 mg / mL rutin standard solution with anhydrous methanol. Accurately pipette 0.0 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, 1.0 mL, and 1.2 mL of the standard solution into 25 mL stoppered test tubes. Add 0.3 mL of 5 wt% sodium nitrite, shake well, and let stand for 6 min. Then add 0.3 mL of 10 wt% aluminum nitrate, shake well, and let stand for 6 min. Add 4 mL of 4 wt% sodium hydroxide solution, dilute to 10 mL with distilled water, shake well, and let stand for 15 min. Measure the absorbance at 510 nm. Plot the standard curve with rutin concentration as the x-axis and absorbance as the y-axis.

[0071] (2) Determination of total flavonoids in the sample: Accurately transfer 1.0 mL of the dried flower extract extracted with eutectic solvent in Examples 1-7 into a 25 mL test tube, and determine the absorbance value according to the method in (1) above. Find the corresponding value in the rutin standard curve based on the absorbance value. The total flavonoid content of the sample is expressed as the relative content of rutin (g CAE / 100g).

[0072]

[0073] The test results for total phenols and total flavonoids are shown in Table 1.

[0074] Table 1

[0075] Example 1 0.1582 Example 2 -0.1875 Example 3 0.0062 0.0034 Example 4 0.0222 Example 5 0.0373 Example 6 0.049

[0076] 3. Antioxidant properties:

[0077] (1) Preparation of standard curve: Take 0.1 mL of ascorbic acid standard solutions of different concentrations (0 mg / mL, 0.2 mg / mL, 0.4 mg / mL, 0.6 mg / mL, 0.8 mg / mL, and 1 mg / mL) into stoppered test tubes, add 5 mL of mixed solution (containing 0.6 mol / L sulfuric acid, 28 mmol / L sodium phosphate, and 4 mmol / L ammonium molybdate) to each tube, stopper the tubes, heat them in a 95℃ water bath for 90 min, remove them and cool them to room temperature, measure the absorbance at a wavelength of 695 nm, and plot the standard curve. The results are shown in Table 2.

[0078] Table 2

[0079]

[0080] By analyzing the ascorbic acid-related data above, a standard curve was obtained. The equation is as follows:

[0081] Y = 48756X - 0.0122, R 2 =0.9961

[0082] (2) Determination of antioxidant properties of the sample: Take 0.1 mL of dried flower extract extracted by eutectic solvent in Example 1 and 0.1 mL of blank eutectic solvent into a 10 mL test tube, and determine the absorbance value according to the method in (1) above. Find the amount of Vc equivalent to 1 mg of sample on the ascorbic acid standard curve, that is: X mg Vc / mg sample. The results are shown in Table 3.

[0083] Table 3

[0084]

[0085] After conversion, 0.1 mL of the dried flower extract extracted with the eutectic solvent in Example 1 is equivalent to 0.010 mg Vc / mg sample.

[0086] (4) Determination of total reducing power: Take 0.2 mL of the dried flower extract extracted with the eutectic solvent in Example 1, add 1 mL of PBS (pH 6.6) and 1 mL of K3[Fe(CN)6] (1 wt%), heat in a 50℃ water bath for 20 min, remove and rinse with running water to room temperature, add 1 mL of C2HCl3O2 solution (10 wt%), centrifuge at 3000 r / min for 10 min, take 2 mL of the supernatant, add 2 mL of deionized water and 0.4 mL of FeCl3 solution (0.1 wt%), mix well, let stand at room temperature for 10 min, and then measure the absorbance at 700 nm. Use Vc as a positive control and the eutectic solvent as a blank control. Replace the dried flower extract with Vc and blank eutectic solvent. Repeat the operation 3 times for each sample. The test results are shown in Table 4.

[0087] Table 4

[0088]

[0089] As shown in the table, when the extract concentration is 3.164 × 10⁻⁶, -4 At g / g, the reducing power of the extract was lower than that of vitamin C but higher than that of the blank.

[0090] 4. Antibacterial effect test: (1) Seed culture preparation: Two loops of colonies were picked from activated Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Bacillus subtilis plates and inoculated into 50 mL of liquid culture medium. The culture was labeled and placed in a constant temperature incubator at 37℃ with shaking at 180 r / min for 15 h. The culture was stopped when the absorbance at 620 nm wavelength on a spectrophotometer reached 0.8.

[0091] Wash the activated Aspergillus niger agar plates with sterile water, collect the bacterial suspension, draw up the bacterial suspension with a syringe and filter the bacterial suspension with a needle filter, take an appropriate amount of filtrate and drop it onto a hemocytometer and place it under an optical microscope to count the number of bacterial spores.

[0092] Cells per milliliter = Average number of cells per small square × 4 × 10 6 ×Dilution factor

[0093] With a spore count of 10 5 -10 7 The bacterial solution was used as the seed solution.

[0094] (2) Antibacterial effect test of extract: Filter paper was punched into 0.6 cm diameter discs and placed in a petri dish for sealing and sterilization. Under aseptic conditions, the filter paper discs were immersed in the sample solution for 40 minutes using sterile forceps and then set aside.

[0095] Under aseptic conditions, add 1 mL of bacterial seed culture to every 300 mL of sterilized culture medium cooled to 60°C. After thorough mixing, pour the mixture into sterile petri dishes. Once the culture medium has cooled and solidified, place a filter paper soaked in the dried flower extract extracted with the eutectic solvent of Example 1 onto the solidified test plate (holding it briefly against the edge of the container to filter out excess solution). Press it firmly with tweezers to ensure it adheres tightly to the culture medium. Perform two parallel tests for each sample. After standing for 20 minutes, incubate flat in a 37°C incubator for 17 hours. Measure the size of the inhibition zone and record the results by photograph.

[0096] Under aseptic conditions, add 1 mL of pre-counted Aspergillus niger seed solution to every 300 mL of sterilized culture medium cooled to 60°C. After thorough mixing, pour the solution into sterile petri dishes. Place sterilized Oxford cups on a plate containing culture medium and gently press them down to ensure they are in contact with the petri dish without any gaps. Several Oxford cups can be placed on each plate. After cooling and solidifying, place the plates flat in a 37°C incubator for 4 days.

[0097] Pour the sterilized Aspergillus niger medium into a sterilized petri dish. After it cools and solidifies, add 1 mL of the dried flower extract extracted with the eutectic solvent in Example 1 to the plate. Spread the sample evenly with a sterilized spreader. Place the Aspergillus niger medium cylinder cultured in the Oxford cup into the plate with the sample. Let it stand for 20 minutes, then place it flat in a 37°C constant temperature incubator for 3 days. Observe the growth of Aspergillus niger and take pictures to record the results.

[0098] Immerse the filter paper discs in blank eutectic solvent and the dried flower extracts extracted with the eutectic solvent of Example 1 for 40 minutes. When removing them, hold them briefly at the edge of the container to filter out excess liquid. Place the filter paper discs on the solidified test bacterial plate and press them firmly with tweezers to ensure they adhere well to the culture medium. After standing for 20 minutes, incubate them horizontally in a 37°C incubator for 17 hours. Measure the size of the inhibition zone, photograph and record the results. The control group for *Aspergillus niger* was prepared using the same inhibition procedure as *Aspergillus niger*. The test results for *Escherichia coli*, *Staphylococcus aureus*, *Bacillus subtilis*, and *Bacillus cereus* are shown in Table 5, and the test results for *Aspergillus niger* are shown in Table 6.

[0099] Table 5

[0100]

[0101] As shown in the table, the extract has inhibitory effects on Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Bacillus cereus. The inhibitory effect on Escherichia coli is the strongest, while the inhibitory effect on Staphylococcus aureus is relatively weak.

[0102] Table 6

[0103]

[0104]

[0105] Note: The effect of the extract on the growth of Aspergillus niger after 72 hours could not be measured to determine the diameter of the Aspergillus niger growth.

[0106] As shown in the table, the extract had a greater growth-promoting effect on Aspergillus niger than the control; however, the growth-promoting effect on Aspergillus niger decreased with increasing extract concentration.

[0107] 5. Free Radical Scavenging Test: The free radical (DPPH) scavenging effect of the dried flower extract extracted with a eutectic solvent in Example 1 was tested according to "T / SHRH 006-2018 Cosmetics - Experimental Method for Free Radical (DPPH) Scavenging". Water was used as the diluent. The test results are shown in Table 7.

[0108] 6. Elastase Inhibition Rate Test: Solution Preparation: Buffer: Mix 50 mL of 0.1 mol / L Tris solution (12.114 g / L) with 29.2 mL of 0.1 mol / L hydrochloric acid, then dilute with water to 100 mL to obtain Tris-HCl buffer (pH 8.0); Elastase Solution: Add 1 mg of elastase to 1 mL of water to prepare a 30 U / mL stock solution, store at -20℃ for later use, and dilute with buffer 10 times to 3 U / mL before use; Substrate Solution: Add 1 mg of N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroaniline to 2 mL of water to prepare a substrate solution with a concentration of 0.5 mg / mL.

[0109] Take a 96-well plate, add the reagents according to the steps in the table below and incubate. After incubation, use a microplate reader to measure the absorbance at 410 nm.

[0110]

[0111] Inhibition rate (%) = [1 - (A1 - A2) / (B1 - B2)] × 100%

[0112] In the formula, A1, A2, B1, and B2 are the absorbance at 410 mm for the corresponding groups.

[0113] The control group consisted of a eutectic solvent solution comprising a eutectic solvent and water in a volume ratio of 1:1; the eutectic solvent included citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol in a volume ratio of 2:1:1:6. The experimental group consisted of the dried flower extract from Example 1. The diluent was water. The test results are shown in Table 7.

[0114] 7. Tyrosinase Inhibition Test: Referring to "T / SHRH 015-2018 Cosmetics - Experimental Method for Tyrosinase Activity Inhibition", the inhibitory effect of the dried flower extract extracted with a eutectic solvent in Example 1 on tyrosinase in cosmetics was tested. Water was used as the diluent. The test results are shown in Table 7.

[0115] Table 7

[0116]

[0117] 8. Irritation Test: Referring to "T / SHRH 011-2018 Cosmetic Eye Irritation Test (Chicken Embryo Viral Allantoic Membrane Test)," the skin irritation of the dried flower extract extracted with a eutectic solvent in Example 1 when applied to cosmetics was tested. The control group consisted of a eutectic solvent solution comprising a eutectic solvent and water in a volume ratio of 1:1; the eutectic solvent included citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol in a volume ratio of 2:1:1:6. The experimental group consisted of the dried flower extract from Example 1. The diluent was water. The test results are shown in Table 8.

[0118] Score (ES) determination. ES≤4 means non-irritation, 4<ES≤12 means mild irritation, 12<ES<16 means moderate irritation, and ES≥16 means severe irritation.

[0119] Table 8

[0120] Example 1 ES=0 Non-irritating

[0121] Figure 17 is the chorioallantoic membrane test image of the control group at 0 s of stimulation, Figure 18 is the chorioallantoic membrane test image at 180 s of stimulation; Figure 19 is the chorioallantoic membrane test image of the Example at 0 s of stimulation, Figure 20 is the chorioallantoic membrane test image of Example 1 at 180 s of stimulation.

Claims

1. A method for extracting dried flower extracts, characterized in that, Includes the following steps: (1) Crush the dried flowers, add them to a eutectic solvent, add water, and shake well; (2) Then place it in an ultrasonic cell disruptor for ultrasonication; (3) Then transfer it to a constant temperature shaking incubator for constant temperature reaction; (4) Finally, filter the sample, collect the filtrate, and obtain the dried flower extract for testing the content of active ingredients; The raw materials for preparing the eutectic solvent are a combination of citric acid, ascorbic acid, 2-hydroxypropionic acid, and glycerol in a weight ratio of 2:1:1:(5-10). The weight ratio of the dried flowers to the eutectic solvent is 1:(5-50); The reaction temperature in step 3 is 15-35℃, the reaction time is 20-30h, and the oscillation frequency in step 3 is 100-190r / min. In step 4, the filtration method is gauze filtration, using 7-10 layers of gauze.

2. The method for extracting dried flower extract according to claim 1, characterized in that, The method for preparing the eutectic solvent includes the following steps: adding an ionizable substance to an organic compound, heating it in a water bath to 75-85°C, stirring continuously during the heating process until the mixture is homogeneous, and then cooling it for later use.

3. The method for extracting dried flower extract according to claim 1, characterized in that, In step 1, the amount of water added is 10-60% of the mass of the dried flowers.

4. A dried flower extract, characterized in that, The dried flower extract was prepared according to the extraction method described in any one of claims 1-3.

5. An application of the dried flower extract prepared according to claim 4, characterized in that, It is used in food, medicine, beverages, and cosmetics.

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