Bacillus sonorensis from snake and application thereof

Abstract

The application discloses a Sphingobium sochongense (XZX-7) strain isolated and screened from healthy snakes, and has a preservation number of CCTCC NO: M 20232010. Cetobacterium somerae The strain has strong antioxidant capacity, and animal experiments prove that the Sphingobium sochongense XZX-7 also has the effects of slowing down animal weight growth, liver fat accumulation and liver damage induced by high-fat diet, simultaneously slowing down animal blood sugar increase induced by high-fat diet and improving insulin resistance capacity, and has good effects in improving animal low-temperature tolerance.

Description

Technical Field

[0001] This invention belongs to the field of probiotic technology, specifically relating to a snake-derived Cetacea strain and its applications. Background Technology

[0002] Probiotics are widely recognized for their numerous health benefits, playing a positive role in strengthening the intestinal barrier, enhancing immunity, regulating host metabolism, and boosting antioxidant capacity. *Cetobacterium somerae* is a symbiotic bacterium found in many fish and reptiles. Some existing studies have shown that *Cetobacterium somerae* can exert antioxidant functions, improving the antioxidant capacity of zebrafish. Other studies have indicated that adding *Cetobacterium somerae* fermentation products to the diet can improve the gut health of tilapia and enhance its resistance to pathogens.

[0003] Overweight or obesity refers to an abnormal health condition caused by excessive accumulation of body fat. Obesity is a major risk factor for many non-communicable diseases and is closely related to the occurrence of diabetes, hypertension, and cardiovascular diseases. Furthermore, the distribution of fat is also closely linked to many diseases. Body fat distribution not only affects dyslipidemia but is also associated with insulin resistance and gallstones. Currently, obesity rates are rising rapidly in many countries worldwide, making obesity prevention and control a critical public health issue that urgently needs to be addressed. Current methods for treating obesity, such as surgery and medication, all have side effects, and restricting diet and exercise for fat loss is difficult to maintain. Therefore, there is an urgent need for safe and effective nutritional solutions. The gut microbiota is closely related to the host's metabolism, regulating many metabolic processes, including energy homeostasis, purine metabolism, amino acid metabolism, carbohydrate metabolism, and lipid metabolism. Probiotics can alter the host's metabolism by influencing energy regulation and chronic inflammatory states associated with obesity; therefore, gut microbiota intervention is a feasible strategy for treating obesity.

[0004] Ambient temperature has a significant impact on human survival and health, while extreme environments in some regions can threaten human health. Low temperatures, as one of the most common stressors in cold regions, can affect the body's neuroendocrine, physiological, biochemical, and immune systems. Low temperatures can cause decreased sensation in the skin and extremities, reduced joint flexibility, and weakened skeletal muscle coordination, leading to some motor injuries. Existing research indicates that microbiome remodeling is an important factor in the induction of beige fat during cold periods and a key factor in promoting energy absorption by increasing intestinal absorptive surface area, thereby coordinating overall energy homeostasis during periods of increased energy demand. Summary of the Invention

[0005] The purpose of this invention is to provide a snake-derived strain of *Solanum lyratum*, which has strong antioxidant capacity and can alleviate obesity, elevated blood lipids, antioxidant enzyme imbalance and liver pathological damage in mice caused by long-term high-fat diet, improve glucose and lipid metabolism, and also has a good effect on improving the animals' cold tolerance.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] Cetobacterium somerae XZX-7, with accession number CCTCC NO: M20232010.

[0008] Application of *S. serpentine-derived* XZX7 in slowing down high-fat diet-induced weight gain, liver fat accumulation or liver damage, dyslipidemia, and hyperglycemia in animals: In a specific embodiment of the present invention, C57BL / 6 male mice were fed a high-fat diet continuously, resulting in obesity and metabolic disorders. The experiment showed that adding *S. serpentine-derived* XZX-7 could significantly slow down weight gain in mice, reduce liver index and fat index, and alleviate the negative effects of high-fat diet-induced serum biochemical and antioxidant enzyme imbalances, liver pathological damage, and glucose metabolism disorders.

[0009] Application of snake-derived Sodomella XZX-7 in improving the hypothermia tolerance of animals: In a specific embodiment of the present invention, mice were exposed to a low temperature environment of 4°C after being supplemented with Sodomella XZX-7 for 3 consecutive weeks. Compared with the control group, the body temperature of the mice supplemented with Sodomella XZX-7 was significantly higher than that of the control group. Sodomella XZX-7 improved the hypothermia tolerance of the mice.

[0010] Compared with the prior art, the present invention has the following advantages:

[0011] The *Solanum lysodeoxycholicum* XZX-7 strain provided by this invention is the first strain isolated and screened from healthy snakes and applied to mammals for the first time. This strain has strong antioxidant capacity. In addition, animal experiments have found that it also has multiple probiotic functions such as improving glucose and lipid metabolism and antioxidation. It can alleviate the negative effects of high-fat diets such as obesity, serum biochemical and antioxidant enzyme disorders, liver pathological damage, and glucose metabolism disorders. It also has a good effect on improving the hypothermia tolerance of animals. *Solanum lysodeoxycholicum* XZX-7 has the potential to be a probiotic and has a very broad application prospect. Attached Figure Description

[0012] Figure 1 Gram staining results for Solomonella XZX-7.

[0013] Figure 2 Phylogenetic tree of Cetacea sorogenesis XZX-7.

[0014] Figure 3 The results show the in vitro antioxidant activity of *Saccharitobacter spp.* XZX-7.

[0015] Figure 4 The trend of weight gain and weight increment of mice in each group in Example 2 are shown.

[0016] Figure 5 The values ​​represent the liver index and the percentage of body weight of fat in each part of the mice in Example 2.

[0017] Figure 6 The concentration levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) in the serum of mice in each group in Example 2.

[0018] Figure 7 The concentration levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in the serum of mice in each group in Example 2.

[0019] Figure 8 The fasting blood glucose (FBG) and insulin resistance index (HOMA-IR) of mice in each group in Example 2 are shown.

[0020] Figure 9 The results of HE staining of mouse liver sections in each group in Example 2 are shown.

[0021] Figure 10 HE staining results of brown adipose tissue sections from mice in each group in Example 2.

[0022] Figure 11 HE staining results of inguinal fat sections from mice in each group in Example 2.

[0023] Figure 12 HE staining results of epididymal fat sections from each group of mice in Example 2.

[0024] Figure 13 The values ​​represent the weight gain and average daily food intake of the two groups of mice in Example 3.

[0025] Figure 14 The body temperature changes of the two groups of mice in Example 3 are shown. Detailed Implementation

[0026] The present invention will now be described clearly and completely. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. The following examples are for illustrative purposes only and should not be considered as limiting the scope of the invention. Where specific conditions are not specified in the examples, conventional conditions or conditions recommended by the manufacturer shall apply.

[0027] Example 1: Isolation, identification, and preservation of snake-derived *Saccharitoidea*

[0028] 1. Isolation and Identification of *Saccharitoidea*

[0029] The applicant collected intestinal contents from *Elaphe carinata* (King Rat Snake) at a large-scale breeding base in Hubei Province for the isolation and screening of *S. spp.* The specific method involved placing the intestinal contents sample in sterile PBS solution, vortexing to homogenize, and serially diluting the sample with sterile PBS solution. 100 μL of the appropriately diluted mixture was then evenly spread onto GAM agar plates. All steps were performed in an anaerobic incubator. The plates were then incubated at 25°C for 48 hours. Single colonies suspected to be *S. spp.* were picked, purified, cultured, and Gram-stained. Figure 1 The strain is numbered XZX-7.

[0030] To further identify the strain, genomic DNA was extracted from strain XZX-7, amplified using universal primers for 16S rDNA, and sequenced to obtain the 16S rDNA gene sequence. BLAST analysis was performed on the NCBI website, and a phylogenetic tree was constructed. Figure 2 The results showed that strain XZX-7 belongs to Cetobacterium somerae in the Cetobacterium group. This strain was deposited at the China Center for Type Culture Collection on October 25, 2023, with accession number CCTCC NO: M 20232010, deposit address: Wuhan University, Wuhan, China, and classified as Cetobacterium somerae.

[0031] 2. Antioxidant capacity of Somdej Cetacea XZX-7

[0032] The antioxidant capacity of probiotics can be verified through a series of in vitro experiments, such as hydroxyl radical scavenging activity, DPPH radical scavenging activity, and superoxide anion radical scavenging activity. *Cetacea somnifera* XZX-7 was inoculated at a 2% inoculum in GAM liquid medium and cultured at 37°C in an anaerobic environment for 24 hours. The culture was then centrifuged at 4°C and 6000 rpm for 10 minutes, and the supernatant was collected and filtered through a 0.2 μm filter membrane to obtain the fermentation supernatant for antioxidant capacity testing.

[0033] Hydroxyl radical scavenging activity: Accurately transfer 50 μL of fermentation supernatant, add 50 μL FeSO4 (6 mmol / L) and 100 μL H2O2 (6 mmol / L), shake thoroughly, and let stand for 10 min; add 50 μL salicylic acid (6 mmol / L), shake thoroughly, and let stand at room temperature for 30 min. Measure the absorbance at 510 nm, repeat 3 times, and take the average value. The hydroxyl radical scavenging rate is calculated using the formula: [(A0-A1) / A0]×100%, where A0 is the blank, with 50 μL of distilled water replacing the absorbance value of the sample; A1 is the absorbance value of the fermentation supernatant.

[0034] DPPH radical scavenging activity: Take 2 mL of fermentation supernatant, add 1 mL of 0.2 mmol / L 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) anhydrous ethanol solution, mix well, let stand at room temperature in the dark for 30 min, react in the dark for 30 min, then centrifuge at 9000 r / min for 10 min, and measure the absorbance of the supernatant at 517 nm. Repeat three times. The DPPH radical scavenging rate is calculated as: [1-(A1-A2) / A0]×100%, where A0 is the absorbance of an equal volume of anhydrous ethanol replacing the sample; A1 is the absorbance of the fermentation supernatant; and A2 is the absorbance of an equal volume of anhydrous ethanol replacing the DPPH ethanol solution.

[0035] Superoxide anion radical scavenging activity: Take 1 ml of fermentation supernatant, add 3 ml of 0.05 mol / L Tris-HCl, shake and mix well, incubate at 25℃ for 20 min, then add 0.4 ml of 25 mmol / L pyrogallol, shake and mix well, incubate at room temperature for 4 min, and finally add 0.5 ml of 8 mol / L HCl. Measure the absorbance at 325 nm, repeat 3 times. The superoxide anion radical scavenging rate is calculated as: [1-(A1-A2) / A0]×100%, where A0 is the absorbance value of blank GAM medium replacing the sample, A1 is the absorbance value of the fermentation supernatant, and A2 is the absorbance value of an equal volume of double-distilled water replacing the pyrogallol solution.

[0036] The measurement results are as follows Figure 3 As shown, the calculated DPPH radical scavenging rate of the fermentation supernatant of *Saccharitoideus* XZX-7 was 80.00%, the hydroxyl radical scavenging rate was 81.30%, and the superoxide anion radical scavenging rate was 98.59%, indicating strong in vitro antioxidant capacity.

[0037] Example 2: Effect of Soxhlete's XZX-7 on high-fat diet-induced obesity in mice.

[0038] 1. Experimental Methods

[0039] This experiment was conducted from June 24, 2023 to August 19, 2023 at the Experimental Animal Center of Huazhong Agricultural University. Forty-eight SPF-grade, 6-week-old female C57BL / 6 mice (purchased from the Experimental Animal Center of Huazhong Agricultural University) were used and housed in a mouse room at 23±1℃, with 12 hours of light per day (8:00 AM to 8:00 PM), and free access to food and water. The formal experiment began after one week of acclimatization. The mice were randomly divided into four groups (n=12): a normal control group (NC group), a Sodomella xanthoceras sorbi XZX-7 group (XZX-7 group), a high-fat diet group (HFD group), and a high-fat diet + Sodomella xanthoceras sorbi XZX-7 group (HFD+XZX-7 group).

[0040] The NC and XZX-7 groups were fed a normal diet, while the HFD and HFD+XZX-7 groups were fed a high-fat diet. During the 8-week intervention, mice had free access to food and water. Mice in the XZX-7 and HFD+XZX-7 groups were administered 1.0 × 10⁻⁶ mg / L via gavage at 10:00 AM daily. 9 Mice in the NC and HFD groups were administered 0.2 mL of CFU / mL *Cetaceae XZX-7* bacterial suspension via gavage at the same time. The high-fat diet contained 24.2% protein, 40.1% carbohydrates, and 27.4% fat by mass, with corresponding 19.8%, 31.7%, and 48.5% of total calories, respectively.

[0041] The mice's food intake was recorded daily, and their weight was measured every three days. On day 56, fasting blood glucose was measured using the tail-cutting blood collection method. Blood was then collected by removing the eyeballs. The mice were euthanized by cervical dislocation, their trunks were fixed, and they were dissected along the midline of the abdomen. The thoracic and abdominal cavities were opened, and the target tissues and organs were collected: liver, scapular fat, inguinal fat, and epididymal fat. The tissues were weighed, and half of the tissues were preserved in fixative for later pathological section observation. The other half was removed during dissection, immediately placed in liquid nitrogen, and then stored in a -80°C freezer. The blood obtained by the above method was allowed to stand at room temperature in EP tubes for more than one hour, and then centrifuged at 3000 rpm for 10 minutes to obtain serum.

[0042] 2. Measurement Method

[0043] 2.1 Body weight, food intake, and organ index

[0044] Weight gain (g) = Final weight - Initial weight

[0045] Liver index (%) = (liver weight / body weight) × 100

[0046] Epididymal fat percentage (%) = (epididymal fat weight / body weight) × 100

[0047] Groin fat percentage (%) = (groin fat weight / body weight) × 100

[0048] Brown fat percentage (%) = (weight of brown fat / body weight) × 100

[0049] 2.2 Determination of blood biochemical indicators, fasting blood glucose, insulin sensitivity index, and resistance index, etc.

[0050] Serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured using kits provided by Shenzhen Mindray Animal Medical Technology Co., Ltd., following the instructions, and on a Mindray BS240-VET7020 fully automated biochemical analyzer.

[0051] Fasting blood glucose (FBG) was measured using a Sinocare blood glucose meter, and the level of insulin (FINS) in mouse serum was determined using an enzyme-linked immunosorbent assay (ELISA) kit. The operation steps and sample loading volume were performed in accordance with the kit instructions.

[0052] The homeostasis model of insulin resistance (HOMA-IR) = FBG × FINS / 22.5

[0053] 2.3 Histopathological observation

[0054] Each adipose tissue was fixed with Seville fat fixative, and the liver tissue was fixed with 100 mL / L formalin. The tissues were washed, dehydrated in a gradient of alcohol, cleared, embedded in paraffin, sectioned, mounted, dewaxed and rehydrated, stained with hematoxylin and eosin, and mounted. The changes in the liver tissue were observed under a light microscope.

[0055] 2.4 Data Processing and Analysis

[0056] One-way ANOVA or t-tests were performed using GraphPad Prism 9.5.1 software. All data are expressed as mean ± standard deviation (Mean ± SD). p > 0.05 indicated no statistical difference, *p < 0.05 indicated a statistical difference, **p < 0.01 indicated a significant difference, and ***p < 0.001 and ****p < 0.0001 indicated extremely significant differences. GraphPad Prism 9.5.1 software was used for plotting.

[0057] 3. Results Analysis

[0058] 3.1 Body weight, food intake, and organ index

[0059] like Figure 4As shown, all groups of mice were in their growth phase and their body weight was increasing. The body weight of the HFD group was consistently higher than that of the NC group, while the body weight of the HFD+XZX-7 group was significantly lower than that of the HFD group, indicating that *S. solani* XZX-7 inhibited body weight gain. There was essentially no difference in body weight between the NC and XZX-7 groups, indicating that *S. solani* XZX-7 had no effect on the body weight of mice on a normal diet. After 8 weeks of dietary intervention, compared with their initial body weight, the NC group mice gained an average of 2.13g, and the HFD group mice gained an average of 6.05g, indicating that a high-fat diet can lead to a significant increase in body weight in mice. The HFD+XZX-7 group gained an average of 2.99g, which was significantly lower than that of the HFD group. These results indicate that *S. solani* XZX-7 intervention can significantly slow down body weight gain. Therefore, adding *S. solani* XZX-7 has the ability to promote weight loss.

[0060] Table 1 shows the food intake of mice in each group during the feeding period. There was no significant difference in food intake among the groups, indicating that *Sodomella solani* XZX-7 had no negative impact on mouse appetite. Combined with daily observations of the mice's physiological condition during the feeding period, all mice were in normal condition and relatively active; no abnormalities such as lethargy, weight loss, diarrhea, vomiting, or death were observed. This indicates that *Sodomella solani* XZX-7 has no toxic effect on mice.

[0061] Liver indices for each group as follows Figure 5 As shown, the liver index of mice in the HFD group was significantly higher than that in the NC group, while that in the HFD+XZX-7 group was significantly lower than that in the HFD group, and could basically return to normal levels. This indicates that a high-fat diet leads to fat accumulation, edema, or hyperplasia and hypertrophy in the liver tissue of mice, and that *Cetacea solvae* XZX-7 can intervene in and alleviate this phenomenon.

[0062] like Figure 5 As shown, the epididymal and groin fat percentages of mice in the HFD group were significantly higher than those in the NC group, while those in the HFD+XZX-7 group were significantly lower than those in the HFD group. Furthermore, the brown fat percentage in the HFD+XZX-7 group was significantly lower than that in the HFD group, indicating that *Solanum lysodeoxycholicum* XZX-7 can reduce subcutaneous and epididymal fat accumulation induced by a high-fat diet.

[0063] Table 1 Comparison of food intake in mice

[0064]

[0065] 3.2 Serum biochemical indicators

[0066] like Figure 6As shown, serum ALT, AST, and GGT levels in the HFD group were significantly higher than those in the NC group, and ALP levels were also higher, but the differences were not statistically significant. Intervention with *S. spp.* XZX-7 significantly reduced serum ALT, ALP, AST, and GGT levels. This indicates that *S. spp.* XZX-7 has a certain repairing effect on liver function damage caused by a high-fat diet and can alleviate liver damage to some extent.

[0067] like Figure 7 As shown, serum TC, TG, and LDL-C levels in the HFD group were significantly higher than those in the NC group. Compared with the HFD group, serum TC, TG, and LDL-C levels in the HFD+XZX-7 group were significantly lower. Serum HDL-C levels in the HFD group showed a decreasing trend compared to the NC group, while serum HDL-C levels in the HFD+XZX-7 group were significantly higher than those in the HFD group. This indicates that obese mice induced by a high-fat diet exhibit dyslipidemia and lipid metabolism disorders. Intervention with *Cetacea somnifera* XZX-7 can improve these phenomena to some extent, preventing excessively high blood lipids and slowing lipid accumulation.

[0068] 3.3 Fasting blood glucose and insulin resistance index

[0069] like Figure 8 As shown, after 8 weeks of high-fat diet feeding, the fasting blood glucose (FBG) and HOMA-IR scores of mice in the HFD group were significantly higher than those in the NC group and the HFD+XZX-7 group, while there was no significant difference between the NC group and the HFD+XZX-7 group. This indicates that *S. spp.* XZX-7 intervention reduced blood glucose levels and HOMA-IR scores in high-fat diet mice, improved insulin resistance in the HDF group mice, and enhanced glucose tolerance.

[0070] 3.4 H&E stained sections of liver and fat

[0071] Liver tissue sections were observed under a microscope using H&E staining. For example... Figure 9 As shown, the hepatocytes of the NC group mice were neatly arranged, with clear and intact hepatocyte structure. No fatty degeneration or inflammatory cell infiltration was observed in the cells. Liver sections of the HFD group mice showed a reticular structure, swollen hepatocytes, and observed fatty degeneration, numerous lipid droplets of varying sizes, and inflammatory cell infiltration. Compared with the HFD group, no obvious lipid droplets, necrotic cells, or inflammatory cell infiltration were observed in the hepatocytes of the HFD+XZX-7 group, indicating a near-normal recovery. This demonstrates that *Saccharitolide* XZX-7 intervention has a repairing effect on hepatocytes in mice fed a high-fat diet, which is consistent with the trends in liver biochemical indicators and blood lipid levels among the different groups.

[0072] HE staining results of mouse brown adipose tissue are as follows Figure 10 As shown, significant differences were observed in the sections after HE staining of brown adipose tissue. Compared to the NC group, the brown adipocytes in the HDF group were larger and had a significantly increased intracellular vacuolar content. Conversely, the brown adipocytes and intracellular vacuolars in the HFD+XZX-7 group were significantly smaller. HE staining results of the inguinal and epididymal adipose tissues of various mice are shown below. Figure 11 , Figure 12 As shown in the figure, compared with the normal group, the adipocytes of mice in the HFD group were round and vacuolated and significantly larger. The number of adipocytes in mice in the HFD+XZX-7 group was significantly increased, and the adipocytes were smaller and rounder. These phenomena indicate that intervention with *Saccharitoides spp.* XZX-7 can improve adipocyte hypertrophy caused by a high-fat diet and inhibit obesity in mice.

[0073] Example 3: The role of Somdej Cetacea XZX-7 in enhancing the hypothermia tolerance of mice.

[0074] 1. Experimental Methods

[0075] Twenty-four SPF-grade, 6-week-old female C57BL / 6 mice (purchased from the Experimental Animal Center of Huazhong Agricultural University) were used in the experiment. They were housed in a mouse room at 23±1℃ with 12 hours of light per day (8:00 AM to 8:00 PM), and had free access to food and water. The formal experiment began after one week of acclimatization. The mice were randomly divided into two groups (n=12): a normal control group (Control group) and a *Cetaceae XZX-7* group (XZX-7 group).

[0076] Mice in group XZX-7 were administered 1.0 × 10⁻⁶ gavage daily at 10:00 AM. 9 Mice in the control group were administered 0.2 mL of CFU / mL *C. solanum* XZX-7 bacterial suspension via gavage at the same time. Both groups had free access to food and water for 3 weeks, with daily recording of food intake and weight changes. On day 22, both groups of mice were placed in a 4°C hypothermic environment for 4 hours without food or water. Rectal temperature was measured every 1 hour before and after hypothermic exposure. Data processing and analysis were performed using the same methods as in Example 2.

[0077] 2. Experimental Results

[0078] 2.1 Body weight and food intake

[0079] The food intake and weight gain of the two groups of mice during the feeding period were as follows: Figure 13 As shown, Solomonella XZX-7 had no significant effect on the weight gain and food intake of mice. During the feeding period, all mice remained healthy and active, and no abnormalities such as lethargy, emaciation, diarrhea, vomiting, or death were observed. This indicates that Solomonella XZX-7 has no toxic effect on mice.

[0080] 2.2 Changes in body temperature after exposure to low temperatures

[0081] The changes in body temperature of the two groups of mice after exposure to low temperature are as follows: Figure 14 As shown, during the hypothermia exposure, the XZX-7 group experienced a slower and more gradual decrease in body temperature compared to the control group. At 1 hour and 2 hours of hypothermia exposure, the body temperature of the XZX-7 group was significantly higher than that of the Control group, indicating that *S. sorogenesis* XZX-7 improved the hypothermia tolerance of C57BL / 6 mice and played a role in maintaining body temperature in cold environments.

Claims

1. Snake-borne Somdej whalebacterium ( Cetobacterium somerae XZX-7, characterized in that, Its accession number is CCTCC NO: M 20232010.

2. The use of the snake-derived Sorcocae bacteria XZX-7 as described in claim 1 in the preparation of products that slow down weight gain in animals induced by a high-fat diet.

3. The use of the snake-derived Sorcocae flavus XZX-7 as described in claim 1 in the preparation of products that alleviate high-fat diet-induced fat accumulation or liver damage in animals.

4. The use of the snake-derived Sorcocae XZX-7 as described in claim 1 in the preparation of products that alleviate dyslipidemia induced by a high-fat diet in animals.

5. The use of the snake-derived Sorcocae flavus XZX-7 as described in claim 1 in the preparation of products that slow down the increase in blood glucose induced by a high-fat diet in animals and enhance the insulin resistance of animals.

6. The use of the snake-derived Sorcocae bacteria XZX-7 as described in claim 1 in the preparation of products that improve the low-temperature tolerance of animals.

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