A background-free, specific detection of dichlorvos by CDs-Fe nanoszyme and a preparation method and application thereof

Abstract

The application discloses CDs-Fe nanoscale enzyme for specifically detecting dichlorvos without background, and a preparation method and application thereof. The preparation method of the CDs-Fe nanoscale enzyme comprises the following steps: refluxing a biomass carbon source and alkali in a solvent, filtering after reaction, adding a trivalent iron salt solution dropwise into the filtrate, and precipitating; then, adjusting the pH to the precipitate to be dissolved by using an acid solution, and sequentially stirring, dialysis, filtering and drying, so as to obtain the CDs-Fe nanoscale enzyme. The CDs-Fe nanoscale enzyme provided by the application exhibits excellent peroxidase-like activity in a wide pH range of 3.0-8.0, and dichlorvos can enhance the peroxidase-like activity of the CDs-Fe nanoscale enzyme, so that the specific detection of dichlorvos can be realized. The detection kit prepared by using the CDs-Fe nanoscale enzyme can be detected by photothermal analysis, background interference can be eliminated, the kit has high sensitivity and accuracy for dichlorvos, and has the advantages of good stability, high selectivity, good repeatability, simple preparation, convenient carrying and the like.

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