An ion chromatography column stock solution and its storage method

Abstract

This invention discloses an ion chromatography column storage solution and storage method, belonging to the field of ion chromatography column preservation technology. The ion chromatography column storage solution provided by this invention comprises the following components in parts by weight: 5-10 parts of bactericide, 10-20 parts of bacteriostatic agent, 10-15 parts of stabilizer, and 55-75 parts of deionized water. The bactericide is at least one selected from p-hydroxybenzoic acid ester, a complex quaternary ammonium salt, and isothiazolinone. All components in the above storage solution are common laboratory reagents, low in cost, low in toxicity, and simple to prepare. The storage solution has a wide range of applications, ideal bactericidal and bacteriostatic effects, and good stability, enabling the ion chromatography column to maintain its original performance and thus extending its service life.

Description

Technical Field

[0001] This invention relates to the field of ion chromatography column preservation technology, and more specifically, to an ion chromatography column storage solution and storage method. Background Technology

[0002] Ion chromatography (IC) is a type of high-performance liquid chromatography (HPLC) used to analyze anions and cations. Its separation principle relies on the interaction between the mobile and stationary phases (the column is filled with numerous ion exchange molecules as the stationary phase for ion separation, and the eluent serves as the mobile phase). Due to the different magnitudes of this interaction, different ions have different residence times in the column, eluing sequentially to reach the detector, thus achieving separation and determination. Ion chromatographs are widely used in environmental, food, power, electronics, biological, pharmaceutical, and chemical industries due to their advantages of fast analysis speed, high detection sensitivity, good selectivity, and the ability to simultaneously detect multiple ions. In ion chromatography equipment, the ion column is one of the core components; the separation of various ions in the sample is completed within the column, and it is generally much more expensive than conventional HPLC columns. Damage to ion columns can lead to irreparable losses. Therefore, the maintenance of ion chromatography columns is crucial. Since ion chromatography columns are prone to drying out when not in use for extended periods, employing appropriate storage methods is essential.

[0003] Therefore, this invention is proposed. Summary of the Invention

[0004] The purpose of this invention is to overcome the defects of the prior art by providing an ion chromatography column storage solution and storage method.

[0005] The technical problem solved by this invention is achieved by the following technical solution.

[0006] The present invention provides an ion chromatography column storage solution comprising the following components in parts by weight: 5-10 parts of bactericide, 10-20 parts of bacteriostatic agent, 10-15 parts of stabilizer, and 55-75 parts of deionized water, wherein the bactericide is at least one of p-hydroxybenzoic acid ester, compound quaternary ammonium salt, and isothiazolinone.

[0007] The present invention also provides a method for storing an ion chromatography column, comprising: passing a storage solution into the ion chromatography column to fill the entire ion chromatography column with the storage solution.

[0008] The present invention has the following beneficial effects:

[0009] This invention provides an ion chromatography column storage solution and storage method. The ion chromatography column storage solution provided by this invention comprises the following components in parts by weight: 5-10 parts of bactericide, 10-20 parts of bacteriostatic agent, 10-15 parts of stabilizer, and 55-75 parts of deionized water. The bactericide is at least one selected from p-hydroxybenzoic acid ester, complex quaternary ammonium salt, and isothiazolinone. Using the storage solution provided by this invention can effectively inhibit the growth of bacteria, algae, and molds. Simultaneously, the presence of the stabilizer allows the chromatographic column to be used again after long-term storage without requiring prolonged rinsing and activation with eluent. Both the bactericide and bacteriostatic agent are low in toxicity, environmentally friendly, and possess excellent bacteriostatic and bactericidal properties, maintaining good bactericidal and bacteriostatic effects in both low and high temperature environments. The stabilizer is a commonly used component in ion chromatography eluents; its use as a stabilizer will not contaminate the chromatographic column and can effectively maintain column performance. Detailed Implementation

[0010] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0011] The following is a detailed description of an ion chromatography column storage solution and storage method provided by an embodiment of the present invention.

[0012] The current literature on the use and maintenance of ion chromatography mainly describes the following column storage methods: for short-term storage, the column should be stored in a buffer solution containing 5% methanol or acetone, and both ends of the column should be sealed with a plunger and stored at low temperature; for long-term storage, the column should be stored in a solution containing 100% organic solvent (acetonitrile, methanol, etc.), and both ends of the column should be sealed with a plunger and stored at low temperature.

[0013] Current literature reports on ion chromatography column storage methods require the use of different solutions depending on the storage time and the type of ion chromatography column. Furthermore, there is no universally applicable ion chromatography storage solution for short-term storage. To prevent bacterial growth during long-term storage, existing methods often involve adding 5% acetone or ethanol to the eluent, or using 100% acetonitrile or methanol. While the addition of organic reagents such as acetonitrile, acetone, and methanol can have a bactericidal effect, these organic reagents are highly toxic and may pose health risks during preparation and use.

[0014] Through long-term practice, the inventors have proposed a stock solution applicable to all types of ion chromatography columns. This stock solution mainly contains bactericides, bacteriostatic agents, and stabilizers, comprising the following components in parts by weight: 5-10 parts bactericide, 10-20 parts bacteriostatic agent, and 10-15 parts stabilizer, plus 55-75 parts deionized water. The bactericide is at least one of parabens, complex quaternary ammonium salts, and isothiazolinones. The bactericides and bacteriostatic agents effectively inhibit the growth of bacteria, algae, and molds. The presence of the stabilizer allows the column to be used again after prolonged storage without requiring lengthy rinsing and activation with the eluent. Both the bactericide and bacteriostatic agents are low in toxicity, environmentally friendly, and possess excellent bacteriostatic and bactericidal properties, maintaining good bactericidal and bacteriostatic effects in both low and high temperature environments. The stabilizer is a commonly used component in ion chromatography eluents; its use as a stabilizer will not contaminate the column and will effectively maintain column performance. The specific scheme is as follows:

[0015] In an optional embodiment, the ion chromatography column stock solution comprises the following components in parts by weight: 5-8 parts of bactericide, 12-17 parts of bacteriostatic agent, and 10-12 parts of stabilizer, and 60-70 parts of deionized water, wherein the bactericide is at least one of p-hydroxybenzoic acid ester, compound quaternary ammonium salt, and isothiazolinone.

[0016] In optional embodiments, the bactericide includes, but is not limited to: 2-methyl-4-isothiazolin-3-one (C4H5NOS), dodecyl dimethyl benzyl ammonium chloride (C... 22 H 40 ClN), dodecyl dimethyl benzyl ammonium bromide (C 22 H 40 BrN), ethyl p-hydroxybenzoate (C9H) 10 O3). The above-mentioned bactericides are all widely used bactericides and preservatives with broad-spectrum and highly efficient bactericidal and algaecidal capabilities, effectively killing algae, bacteria, and fungi; they also have certain dispersing and penetrating effects, as well as certain deodorizing and corrosion-inhibiting effects.

[0017] In optional embodiments, the antibacterial agents include, but are not limited to, potassium sorbate (C6H7KO2), sodium sorbate (C6H7NaO2), and sodium salicylate (C7H5NaO3). These antibacterial agents have strong inhibitory effects on molds and putrefactive bacteria, are easily soluble in water, have low toxicity, and good anti-mold effects. Due to their excellent antibacterial and preservative effects, they are widely used and can be used as preservatives.

[0018] In optional embodiments, stabilizers include, but are not limited to: sodium carbonate (Na2CO3), disodium ethylenediaminetetraacetate (C... 10 H 14Sodium carbonate (C6H5Na3O7) is preferred, and sodium carbonate (N2Na2O8) is used. A sodium carbonate solution with a concentration of 10 mmol / L is prepared using chromatographically pure sodium carbonate and deionized water that has been filtered through a 0.45 μm filter membrane and degassed by ultrasonic treatment.

[0019] In an optional embodiment, the bactericide, bacteriostatic agent, and stabilizer components in the storage solution are all of chromatographic purity.

[0020] Meanwhile, this invention also provides a method for storing an ion chromatography column, comprising: passing a storage solution into the ion chromatography column to fill the entire ion chromatography column with the storage solution.

[0021] In an optional embodiment, the prepared stock solution is passed through the ion chromatography column, filling the entire column. The column ends are then sealed tightly with column plugs to prevent the column bed from drying out. The column should be stored in a cool, dark place, such as a refrigerator, and care should be taken to avoid mechanical vibration. It is worth noting that the stock solution provided in this embodiment can generally be stored for 30 days in the ion chromatography column. Before use, it needs to be activated with eluent for half an hour.

[0022] As can be seen from the above, embodiments of the present invention provide an ion chromatography column storage solution and a storage method. The provided ion chromatography column storage solution comprises the following components in parts by weight: 5-10 parts of bactericide, 10-20 parts of bacteriostatic agent, and 10-15 parts of stabilizer, plus 55-75 parts of deionized water. The bactericide is at least one of p-hydroxybenzoic acid ester, a complex quaternary ammonium salt, and isothiazolinone. The components of the storage solution are all common laboratory reagents, low in cost, low in toxicity, and simple to prepare. It does not use highly toxic organic reagents such as acetone, methanol, or acetonitrile, thus avoiding contamination of the chromatographic column and preventing chemical harm to the human body during use. Furthermore, it maintains good bactericidal and stabilizing effects even after long-term storage. Compared with traditional ion chromatography column preservation methods, the method provided in this invention is applicable to all types of ion chromatography columns. The storage solution exhibits ideal and stable bactericidal and bacteriostatic effects.

[0023] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0024] Example 1

[0025] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 20 parts of potassium sorbate, 15 parts of sodium carbonate, and 55 parts of deionized water.

[0026] Example 2

[0027] An ion chromatography column stock solution comprises the following components in parts by weight: 5 parts of 2-methyl-4-isothiazolin-3-one, 10 parts of potassium sorbate, 15 parts of sodium carbonate, and 70 parts of deionized water.

[0028] Example 3

[0029] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 20 parts of potassium sorbate, 10 parts of sodium carbonate, and 60 parts of deionized water.

[0030] Example 4

[0031] An ion chromatography column stock solution comprises the following components in parts by weight: 5 parts of 2-methyl-4-isothiazolin-3-one, 10 parts of potassium sorbate, 10 parts of sodium carbonate, and 75 parts of deionized water.

[0032] Comparative Example 1

[0033] An ion chromatography column stock solution comprises the following components in parts by weight: 20 parts potassium sorbate, 15 parts sodium carbonate, and 65 parts deionized water.

[0034] Comparative Example 2

[0035] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 15 parts of sodium carbonate, and 75 parts of deionized water.

[0036] Comparative Example 3

[0037] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 20 parts of potassium sorbate, and 70 parts of deionized water.

[0038] Comparative Example 4

[0039] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts pyrimethanil, 20 parts potassium sorbate, 15 parts sodium carbonate, and 55 parts deionized water.

[0040] Comparative Example 5

[0041] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 20 parts of potassium sorbate, and 70 parts of deionized water.

[0042] Comparative Example 6

[0043] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one, 15 parts of sodium carbonate, and 75 parts of deionized water.

[0044] Comparative Example 7

[0045] An ion chromatography column stock solution comprises the following components in parts by weight: 20 parts potassium sorbate, 15 parts sodium carbonate, and 65 parts deionized water.

[0046] Comparative Example 8

[0047] An ion chromatography column stock solution comprises the following components in parts by weight: 10 parts of 2-methyl-4-isothiazolin-3-one and 90 parts of deionized water.

[0048] Comparative Example 9

[0049] An ion chromatography column stock solution comprises the following components in parts by weight: 20 parts potassium sorbate and 80 parts deionized water.

[0050] Comparative Example 10

[0051] An ion chromatography column stock solution comprises the following components in parts by weight: 15 parts sodium carbonate and 85 parts deionized water.

[0052] The specific component contents of each embodiment and comparative example are shown in Table 1:

[0053] Table 1: Specific component contents of each embodiment and comparative example

[0054]

[0055]

[0056] Antibacterial efficacy experiments were conducted on the storage solutions prepared using the examples and comparative examples:

[0057] Staphylococcus aureus, Escherichia coli, and Candida albicans were selected as representative bacterial strains to participate in the antibacterial activity test of the stock solutions provided in the embodiments and comparative examples of this invention. 0.1 mL of 10... 3 A CFU / ml bacterial suspension was prepared in a sterile culture dish, and the storage solution provided in the embodiments and comparative examples of this invention was added to the sterile culture dish. The sterile culture dish was then placed in the refrigerator for 7 days and the results were observed. The bacterial count was tested and summarized in Table 2.

[0058] Table 2: Results of antibacterial effect experiment:

[0059]

[0060] As shown in Table 2, the antibacterial effect results indicate that the antibacterial effect is poor when the components of the storage solution change or when only one or more of them are present. The bacterial count measured after the experiments in the examples and comparative examples differed by more than 10 times, indicating that the components of the storage solution provided in the embodiments of the present invention have a good synergistic effect. The storage solution provided in the embodiments of the present invention has a good antibacterial effect, with ideal bactericidal and bacteriostatic effects and good stability. Practice has proven that: passing the prepared storage solution into the ion chromatography column, allowing the storage solution to fill the entire column, and then sealing both ends of the column with column plugs, and storing the column in a low-temperature and light-protected place, can ensure that the ion chromatography column will not grow bacteria and mold under long-term storage conditions. The storage solution can still maintain good bactericidal and stable effects after long-term storage, allowing the ion chromatography column to maintain its original good performance.

[0061] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A stock solution for ion chromatography columns, characterized in that, It consists of the following components in parts by weight: 5-10 parts bactericide, 10-20 parts bacteriostatic agent, 10-15 parts stabilizer, and 55-75 parts deionized water, wherein... The bactericide is at least one of the following: 2-methyl-4-isothiazolin-3-one, dodecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, and ethyl p-hydroxybenzoate; The antibacterial agent is at least one of potassium sorbate, sodium sorbate and sodium salicylate; The stabilizer is at least one of sodium carbonate, disodium ethylenediaminetetraacetate, and sodium citrate.

2. The ion chromatography column stock solution according to claim 1, characterized in that, The stabilizer is sodium carbonate, which is prepared into a sodium carbonate solution with a concentration of 10 mmol / L by mixing sodium carbonate with deionized water that has been filtered through a 0.45 μm filter membrane and degassed by ultrasonic treatment.

3. The ion chromatography column stock solution according to any one of claims 1-2, characterized in that, The bactericides, bacteriostatic agents, and stabilizers mentioned are all chromatographically pure reagents.

4. A method for storing an ion chromatography column, characterized in that, It includes: A stock solution is introduced into an ion chromatography column to fill the entire column, wherein the stock solution is selected from any one of the stock solutions described in claims 1-3.

5. The storage method according to claim 4, characterized in that, The ion chromatography column can be any type of ion chromatography column.

6. The storage method according to claim 4, characterized in that, The stock solution is introduced into the ion chromatography column to fill the entire column. Then, the two ends of the ion chromatography column are sealed with column head plugs to prevent the column bed from drying out. The ion chromatography column is then stored in a cool, dark place, and care is taken to avoid mechanical vibration.

7. The storage method according to claim 6, characterized in that, The ion chromatography column filled with the storage solution was stored in a refrigerator.

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