A pesticide composition containing flufenoxadiazam and use thereof

Abstract

The application belongs to the technical field of pesticide sterilization, and particularly discloses a pesticide composition containing flufenoxadiazam, which comprises active ingredient A and active ingredient B; the active ingredient A is flufenoxadiazam; the active ingredient B is any one of fluquinconazole, ipfentrifluconazole or pyraclostrobin; and the mass ratio of the active ingredient A to the active ingredient B is 1:35-40:1. The pesticide composition has a good control effect on plant rust order diseases, can effectively delay the generation and development of pathogenic bacteria resistance, is safe to crops, and is friendly to the environment.

Description

[0001] This invention application is a divisional application of application number CN 202310553955.9, filed on May 17, 2023, entitled "A pesticide composition containing flufenoxadiazam and its use therein". Technical Field

[0002] This invention belongs to the field of pesticide fungicide technology, and discloses a pesticide composition containing flufenoxadiazam and its uses. Background Technology

[0003] Flufenoxadiazam is a benzoylaniline fungicide with the chemical name 2′-fluoro-4-[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]benzoylaniline and CAS Registry Number 1839120-27-2. Its mechanism of action is as an inhibitor of histone deacetylation (HDAC), and it has excellent control effects on diseases such as soybean rust and corn rust.

[0004] Fluticiconazole, CAS Registry Number: 133855-98-8, international common name: epoxiconazole, belongs to the triazole class of systemic fungicides. It can be absorbed by the stems and leaves of plants and translocated upwards. It is an ergosterol biosynthesis inhibitor, possessing both curative and protective effects. It is a low-toxicity and safe fungicide, with good control effects against diseases such as rust, damping-off, and powdery mildew.

[0005] ipfentrifluconazole is a novel triazole fungicide developed by BASF, with CAS Registry Number 1417782-08-1. It is a C-type fungicide in sterol biosynthesis. 14 - Demethylation inhibitors can effectively prevent and control a variety of pathogenic fungi, such as rust and diseases caused by Scirococcus, and are suitable for a variety of crops, including field crops, cash crops and specialty crops.

[0006] Pyrazosulfuron is a succinate dehydrogenase inhibitor fungicide developed by Syngenta. This fungicide affects the respiratory chain electron transport system of pathogens by acting on protein complex II, hindering their energy metabolism, inhibiting the growth of pathogens, and ultimately leading to their death.

[0007] Rust is a plant disease caused by pathogenic fungi belonging to the order Rustales. It affects the leaves, stems, and fruits of plants. Rust-causing fungi generally cause only localized infection. Affected areas may develop small pustules of different colors, or pustules, cup-shaped growths, or hairy growths due to spore accumulation. Some rust can also cause tumors, rough bark, bushy branches, and twisted branches on the trunk, or lead to leaf drop, scorched tips, and poor growth. In severe cases, spore masses densely cluster, causing the plant to rapidly wither and die due to excessive water evaporation. Rust is widely distributed and highly damaging, commonly found in cereal crops, legumes, and fruit trees. Rust in many crops is global, with some exhibiting large-scale epidemics. Therefore, severe rust outbreaks lead to decreased seed setting rates, seriously affecting yield and crop quality.

[0008] Through extensive experimental research, the applicant discovered that a reasonable combination of flufenoxadiazam with any one of flutriazole, ipfentrifluconazole, or pyraclostrobin has excellent control effects against a variety of rust pathogens, especially wheat rust, soybean rust, and corn rust, with significant synergistic effects, and is safe for the target crops and environmentally friendly. Summary of the Invention

[0009] To achieve the above objectives, the present invention provides a pesticide composition containing flufenoxadiazam, and provides the use of the pesticide composition containing flufenoxadiazam for controlling rust fungal diseases. The pesticide composition of the present invention has a significant synergistic effect against rust fungal diseases, effectively inhibiting the development and progression of pathogen resistance, and reducing the dosage of pesticides used.

[0010] To make the technical solution of the present invention clearer, the present invention adopts the following technical solution: a pesticide composition containing flufenoxadiazam, wherein the pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is flufenoxadiazam, and active ingredient B is any one of flutriafol, ipfentrifluconazole or pyraclostrobin, wherein the mass ratio of active ingredient A to active ingredient B is 1:35 to 40:1;

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:25 to 20:1;

[0012] Furthermore, in the pesticide composition, active ingredient B is flutriafol, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 20:1.

[0013] Furthermore, in the pesticide composition, active ingredient B is flutriafol, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 10:1;

[0014] Furthermore, the active ingredient B in the pesticide composition is ipfentrifluconazole, and the mass ratio of active ingredient A to active ingredient B is 1:25 to 20:1;

[0015] Furthermore, the active ingredient B in the pesticide composition is ipfentrifluconazole, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 10:1;

[0016] Furthermore, in the pesticide composition, active ingredient B is pyraclostrobin, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:1.

[0017] Furthermore, in the pesticide composition, active ingredient B is pyraclostrobin, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 12:1.

[0018] Furthermore, the total weight of the pesticide composition is 100% wt, and the total weight of active ingredient A and active ingredient B accounts for 1% to 80% of the total weight of the pesticide composition;

[0019] Furthermore, in addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0020] Furthermore, the pesticide composition can be prepared into any formulation permitted in agriculture, wherein the formulation is selected from solid or liquid formulations;

[0021] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0022] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0023] Furthermore, the liquid formulation is selected from emulsifiable concentrates, suspensions, or microemulsions, and the solid formulation is selected from water-dispersible granules.

[0024] The present invention also discloses the use of the pesticide composition described above for the prevention and control of crop rust diseases;

[0025] Furthermore, the rust fungal diseases mentioned above include diseases caused by the following pathogens: *Russula*, *Russula*, *Russula*, *Russula*, *Russula*, *Russula*, *Russula*, or *Russula*.

[0026] Furthermore, the crops mentioned are cereal crops, legumes and fruit trees, and the rust fungi are wheat rust, corn rust, pear rust, legume rust or apple rust;

[0027] Furthermore, the rust fungi diseases mentioned are soybean rust, corn rust, or wheat rust;

[0028] Furthermore, the pesticide composition is applied at an effective dose to the pathogen and / or the medium in which it occurs and / or its growth environment.

[0029] The beneficial effects of this invention are as follows:

[0030] 1) The pesticide composition of the present invention rationally combines compounds with different mechanisms of action, and has significant control effects on diseases caused by rust fungi, especially wheat leaf rust, soybean rust and corn rust.

[0031] 2) The pesticide composition obtained by the present invention is superior to single agents in delaying the development of resistance and prolonging pesticide retention;

[0032] 3) When the fungicidal synergistic effect of the pesticide composition or formulation obtained by the present invention is improved, the production cost and usage cost can be reduced, and the crop is safe. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0034] Formulation preparation example:

[0035] Preparation Example 1: 20% flufenoxadiazam·flufenoxatil suspension (1:1)

[0036] Formula: 10% flufenoxadiazam, 10% flutriazole, 2% fatty alcohol polyoxyethylene ether, 4% alkylphenol polyoxyethylene ether phosphate, 2% sodium polycarboxylate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 1% sodium sorbate, 0.5% silicone oil, deionized water to make up the balance;

[0037] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0038] Preparation Example 2: 24% flufenoxadiazam·flufenazole water-dispersible granules (5:1)

[0039] Formula: 20% flufenoxadiazam, 4% flutriafol, 7% sodium lignosulfonate, 2% BX (a type of lignosulfonate), 2% sodium dodecyl sulfate, 7% naphthalene sulfonate formaldehyde condensate, 8% ammonium sulfate, kaolin to make up the balance;

[0040] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0041] Preparation Example 3: 16% flufenoxadiazam·flufenoxatil emulsifiable concentrate (3:1)

[0042] Formula: 12% flufenoxadiazam, 4% flutriafol, 12% DMF, 12% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 15% propylene carbonate, xylene balance;

[0043] Preparation method: According to the formula ratio, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil required by the present invention.

[0044] Preparation Example 4: 20% flufenoxadiazam·ipfentrifluconazole suspension (3:1)

[0045] Formula: 15% flufenoxadiazam, 5% ipfentrifluconazole, 1.5% isotridecyl alcohol polyoxyethylene ether, 1% succinate sulfonate, 3% styrene-phenol polyoxyethylene ether phosphate, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0046] Preparation method: Same as in preparation example 1.

[0047] Preparation Example 5: 20% flufenoxadiazam·ipfentrifluconazole water-dispersible granules (1:4)

[0048] Formula: 4% flufenoxadiazam, 16% ipfentrifluconazole, 3% naphthalene sulfonate formaldehyde condensate, 8% sodium polycarboxylate, 2% sodium dodecyl sulfate, 7% silica, kaolin to make up the balance;

[0049] Preparation method: Same as in preparation example 2.

[0050] Preparation Example 6: 18% flufenoxadiazam·ipfentrifluconazole emulsifiable concentrate (1:2)

[0051] Formula: 6% flufenoxadiazam, 12% ipfentrifluconazole, 8% dimethyl sulfoxide, 10% styrene-phenol polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 20% propylene carbonate, xylene to make up the balance;

[0052] Preparation method: Same as in preparation example 3.

[0053] Preparation Example 7: 22% flufenoxadiazam·pyrazothiamethoxam suspension (1:1)

[0054] Formula composition: 11% flufenoxadiazam, 11% pyrazole naphthalene, 3% naphthalene sulfonate, 1% Gelbert alcohol polyoxyethylene ether, 3% alkyl polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 1% sodium benzoate, 5% propylene glycol, 0.5% silicone defoamer, deionized water to make up the balance;

[0055] Preparation method: Same as in preparation example 1.

[0056] Preparation Example 8: 28% flufenoxadiazam·pyrazothiamethoxam suspension (1:6)

[0057] Formula composition: 4% flufenoxadiazam, 24% pyrazole naphthalene, 1% sodium polycarboxylate, 2% fatty alcohol polyoxyethylene ether, 4% styrene-phenol polyoxyethylene ether phosphate, 1% carboxymethyl cellulose, 1% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.1% sodium sorbate, 0.5% silicone defoamer, deionized water to make up the balance;

[0058] Preparation method: Same as in preparation example 1.

[0059] Preparation Example 9: 32% flufenoxadiazam·pyrazothiamethoxam water-dispersible granules (1:3)

[0060] Formula composition: 8% flufenoxadiazam, 24% pyrazole naphthalene, 10% sodium lignosulfonate, 2% sodium dodecyl sulfate, 5% alkyl naphthalene sulfonate formaldehyde condensate, 2% polyethylene glycol, 5% ammonium sulfate, bentonite to make up the balance;

[0061] Preparation method: Same as in preparation example 2.

[0062] Preparation Example 10: 35% flufenoxadiazam·pyrazothiamethoxam water-dispersible granules (3:4)

[0063] Formula composition: 15% flufenoxadiazam, 20% pyrazole naphthalene, 2% sodium dodecyl sulfate, 6% naphthalene sulfonate formaldehyde condensate, 10% tea saponin, 5% sodium chloride, 3% soluble starch, kaolin to make up the balance;

[0064] Preparation method: Same as in preparation example 2.

[0065] Preparation Example 11: 26% flufenoxadiazam·pyrazothiamethoxam EC (3:1)

[0066] Formulation composition: 19.5% flufenoxadiazam, 6.5% pyrazole naphthalene, 10% EO / PO block copolymer, 10% dimethyl sulfoxide, 8% propylene glycol methyl ether, 1% calcium dodecylbenzenesulfonate, xylene to make up the balance;

[0067] Preparation method: Same as in preparation example 3.

[0068] Preparation Example 12: 13% flufenoxadiazam·pyraclostrobin EC (1:12)

[0069] Formula composition: 1% flufenoxadiazam, 12% pyrazole naphthalene, 10% dimethyl sulfoxide, 10% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% propylene carbonate, and trimethylbenzene to make up the balance;

[0070] Preparation method: Same as in preparation example 3.

[0071] Indoor toxicity test

[0072] Example 1: Indoor activity test against wheat leaf rust

[0073] Test basis: NY / T 1156.15-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 15: Pot Test Method for Control of Wheat Leaf Rust".

[0074] Experimental target: Puccinia recondita Roberge ex Desmaz.f.triticiErikss et Henn., the fungus that causes wheat leaf rust.

[0075] Test crop: Tanmai 98, a wheat variety susceptible to leaf rust, was selected for pot cultivation. 20 seeds were sown in each pot. After emergence, 10 plants were selected and numbered when they reached the 2-leaf and 1-heart stage.

[0076] Experimental reagents: 90% flufenoxadiazam technical grade, 97% flutriafol technical grade, 98% ipfentrifluconazole technical grade, and 92% pyraclostrobin technical grade. All of the above technical grades were provided by the Group's R&D Center.

[0077] Reagent preparation: Dissolve the test reagents in solvent and prepare them into stock solutions for later use.

[0078] The ratio of effective ingredients in the mixture is set as follows:

[0079] flufenoxadiazam: Flufenoxadiazam is available in ratios of 1:35, 1:20, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1, 20:1, and 30:1.

[0080] flufenoxadiazam: ipfentrifluconazole is 1:35, 1:25, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1, 20:1, 30:1, 40:1;

[0081] flufenoxadiazam: Pyrazothiamethoxam is available in ratios of 1:35, 1:20, 1:10, 1:4, 1:2, 1:1, 3:1, 5:1, 10:1, 25:1, and 58:1.

[0082] The mother liquor was then diluted with an aqueous solution containing 0.1% Tween 80, with 5 concentrations designed for each treatment.

[0083] Experimental Method: Inoculation was performed using a spore suspension spray. After inoculation, wheat seedlings were cultured in the dark at 20℃ for 24 hours before being treated with the pesticide. The pesticide was sprayed evenly onto the prepared wheat seedlings and allowed to air dry naturally. Then, the seedlings were cultured at a temperature of 18–22℃ with a light-to-dark ratio of L:D = (12:12) h. A control group without the pesticide was included. Each concentration treatment was replicated four times, with one replicate per pot containing 10 seedlings.

[0084] Data survey: When the incidence rate in the blank control group reaches 80% or higher, the incidence rate of each treatment is investigated in a graded manner.

[0085] The grading method is as follows:

[0086] Grade 0: No spore masses;

[0087] Grade 1: Spore masses account for less than 5% of the total leaf area;

[0088] Grade 3: Spore masses account for 5 to 10% of the total leaf area;

[0089] Grade 5: Spore masses account for 10 to 25% of the total leaf area;

[0090] Grade 7: Spore masses account for 25 to 50% of the total leaf area;

[0091] Grade 9: Spore masses account for more than 50% of the total leaf area.

[0092] Data statistics and analysis:

[0093] The disease index is calculated by formula (1)

[0094]

[0095] Wherein:

[0096] X represents the disease index;

[0097] N i represents the number of diseased leaves at each grade;

[0098] i represents the relative grade value;

[0099] N represents the total number of investigated leaves.

[0100] The control effect is calculated by formula (2)

[0101]

[0102] Wherein:

[0103] P represents the control effect, in %;

[0104] CK represents the disease index of blank control;

[0105] PT represents the disease index of pesticide treatment.

[0106] With reference to the bioassay standard method NY / T 1156.6-2006, the synergistic effect of mixed pesticides is evaluated according to Sun Yunpei's co-toxicity coefficient method (CTC): CTC ≤ 80 indicates antagonism, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The co-toxicity coefficient (CTC) is calculated according to formula (3), formula (4) and formula (5).

[0107]

[0108] Wherein:

[0109] ATI – The measured toxicity index of a mixture;

[0110] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0111] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0112] TTI = TI A ×P A +TI B ×P B ................................(4)

[0113] In the formula:

[0114] TTI – Theoretical toxicity index of the mixture;

[0115] TI A —The toxicity index of drug A;

[0116] P A —Percentage content of drug A in the mixture, in milligrams per liter (mg / L);

[0117] TI B —Toxicity index of Agent B;

[0118] P B —Percentage content of drug B in the mixture, in milligrams per liter (mg / L).

[0119]

[0120] In the formula:

[0121] CTC – Cotoxicity Coefficient;

[0122] ATI – Actual Measured Toxicity Index of Mixtures;

[0123] TTI – Theoretical Toxicity Index of Mixtures.

[0124] The experimental results were calculated using DPS data processing software, and the toxicity regression equations and EC values ​​for single-dose and mixtures with different ratios of the test agents were obtained. 50 The co-toxicity coefficient (CTC) of the two agents with different ratios was calculated, and the optimal ratio of the test agents was screened.

[0125] Results of flufenoxadiazam and flutriafol activity assays against wheat leaf rust virus:

[0126] Table 1. Combined toxicity determination of flufenoxadiazam and flutriafol against wheat leaf rust.

[0127]

[0128]

[0129] Table 1 shows that the combination of flufenoxadiazam and flutriafol has a good fungicidal effect against wheat leaf rust. The combined toxicity assays of different ratios of flufenoxadiazam and flutriafol against wheat leaf rust showed that flutriafol had higher toxicity against wheat leaf rust, with EC50... 50 The EC50 concentration of flufenoxadiazam was 0.1845 mg / L, which was the highest for wheat leaf rust. 50 The concentration was 0.3913 mg / L. When the mass ratio of flufenoxadiazam to flutriafol was 1:35, 1:20, and 35:1, the effect was additive. However, in the range of 1:10 to 20:1, the synergistic effect was observed. The synergistic effect was most significant when the mass ratio of flufenoxadiazam to flutriafol was 1:1, with a co-toxicity coefficient of 194.844.

[0130] Results of flufenoxadiazam and ipfentrifluconazole infecting wheat leaf rust virus:

[0131] Table 2. Combined toxicity assay of flufenoxadiazam and ipfentrifluconazole against wheat leaf rust.

[0132]

[0133] Table 2 shows that the mixture of flufenoxadiazam and ipfentrifluconazole has a good fungicidal effect on wheat leaf rust.

[0134] The combined toxicity assay of flufenoxadiazam and ipfentrifluconazole at different ratios against wheat leaf rust showed that ipfentrifluconazole had higher toxicity against wheat leaf rust, EC50. 50 The EC50 concentration of flufenoxadiazam was 0.1042 mg / L, which was the highest for wheat leaf rust. 50The concentration was 0.3913 mg / L. Flufenoxadiazam and ipfentrifluconazole showed synergistic effects in a mass ratio ranging from 1:25 to 40:1, with the most significant synergistic effect observed at a mass ratio of 3:1, resulting in a co-toxicity coefficient of 197.528.

[0135] Results of flufenoxadiazam and pyraclostrobin assays against wheat leaf rust virus:

[0136] Table 3. Combined toxicity assay of flufenoxadiazam and pyraclostrobin against wheat leaf rust.

[0137]

[0138]

[0139] Table 3 shows that the combination of flufenoxadiazam and pyraclostrobin has a good control effect on wheat leaf rust. The combined toxicity test results of different ratios of flufenoxadiazam and pyraclostrobin against wheat leaf rust showed that pyraclostrobin had higher toxicity against wheat leaf rust, EC50. 50 The EC50 concentration of flufenoxadiazam against wheat leaf rust was 0.0858 mg / L. 50 The concentration was 0.3913 mg / L. When the mass ratio of flufenoxadiazam to pyraclostrobin was 1:35, they showed an additive effect. However, in the range of 1:20 to 58:1, they all showed a synergistic effect. The synergistic effect was most obvious when the mass ratio of flufenoxadiazam to pyraclostrobin was 1:1, with a co-toxicity coefficient of 184.456.

[0140] Example 2: Indoor activity test against soybean rust

[0141] The experiment was conducted according to the "Standard Operating Procedure for Testing the Biological Activity of Pesticides: Fungicides SOP-SC-1120 Soybean Rust Pot Method".

[0142] Experimental target: Soybean rust fungus (Phakopsora pachyrhizi Syd.).

[0143] The test crop was the rust-susceptible soybean variety Liaodou 15, which was planted in pots. Two seeds were sown in each pot, and only one plant was left after germination. When the first pair of true leaves were fully unfolded, the plants were numbered for future use.

[0144] Experimental reagents: 90% flufenoxadiazam technical grade, 97% flutriafol technical grade, 98% ipfentrifluconazole technical grade, and 92% pyraclostrobin technical grade. All of the above technical grades were provided by the Group's R&D Center.

[0145] Preparation of reagents: Select appropriate solvents to prepare stock solutions of the test reagents flufenoxadiazam, flutriazole, ipfentrifluconazole, and pyraclostrobin for later use.

[0146] The ratio of effective ingredients in the mixture is set as follows:

[0147] flufenoxadiazam: Flufenoxadiazam is available in ratios of 1:10, 1:8, 1:4, 1:3, 5:1, 8:1, and 10:1.

[0148] flufenoxadiazam: ipfentrifluconazole is 1:10, 1:8, 1:4, 1:2, 2:1, 4:1, 10:1;

[0149] flufenoxadiazam: Pyrazothiamethoxam is available in ratios of 1:20, 1:12, 1:6, 1:3, 1:1, 3:1, 6:1, and 12:1.

[0150] The mother liquor was then diluted with an aqueous solution containing 0.1% Tween 80, with 5 concentrations designed for each treatment.

[0151] Experimental Method: The prepared pesticide solution was evenly sprayed onto the leaf surface until completely wet, and then placed indoors to air dry naturally. 24 hours after treatment, the leaves were inoculated with spore suspension, air-dried naturally, and then placed in a dark, moist environment at 25℃ and 100% humidity for at least 24 hours. Afterwards, they were placed in a culture room at approximately 25℃ with a light-to-D ratio of L:D = (12:12) h for normal cultivation. A control group without the pesticide was included. Each concentration treatment was replicated four times, with ten plants per replicate.

[0152] Data survey: When the incidence rate in the blank control group reached 80% or higher, the incidence rate in each treatment was investigated in a tiered manner. The tiering method was as follows:

[0153] Level 0: No spore-bearing mass;

[0154] Level 1: The spore mass accounts for less than 5% of the total leaf area;

[0155] Level 3: The spore mass accounts for 6-10% of the total leaf area;

[0156] Level 5: The spore mass accounts for 11-25% of the total leaf area;

[0157] Grade 7: spore masses account for 26% to 50% of the area of the entire leaf;

[0158] Grade 9: spore masses account for more than 50% of the area of the entire leaf.

[0159] Data statistics and analysis:

[0160] The disease index is calculated by formula (1)

[0161]

[0162] Wherein:

[0163] X — disease index;

[0164] N i — number of diseased leaves at each grade;

[0165] i — relative grade value;

[0166] N — total number of investigated leaves.

[0167] The control effect is calculated by formula (2)

[0168]

[0169] Wherein:

[0170] P — control effect, unit: %;

[0171] CK — disease index of blank control;

[0172] PT — disease index of chemical treatment.

[0173] With reference to the bioassay standard method NY / T 1156.6-2006, the synergistic effect of mixed pesticides is evaluated according to Sun Yunpei's co-toxicity coefficient method (CTC): CTC ≤ 80 indicates antagonism, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The co-toxicity coefficient (CTC) is calculated according to formula (3), formula (4) and formula (5).

[0174]

[0175] Wherein:

[0176] ATI — actually measured toxicity index of the mixed agent;

[0177] S — EC of the standard agent 50 , unit: milligram per liter (mg / L);

[0178] M — EC of the mixed agent 50 , unit: milligram per liter (mg / L).

[0179] TTI=TIA ×P A +TI B ×P B ...........................(4)

[0180] In the formula:

[0181] TTI – Theoretical toxicity index of the mixture;

[0182] TI A —The toxicity index of drug A;

[0183] P A —Percentage content of drug A in the mixture, in milligrams per liter (mg / L);

[0184] TI B —Toxicity index of Agent B;

[0185] P B —Percentage content of drug B in the mixture, in milligrams per liter (mg / L).

[0186]

[0187] In the formula:

[0188] CTC – Cotoxicity Coefficient;

[0189] ATI – Actual Measured Toxicity Index of Mixtures;

[0190] TTI – Theoretical Toxicity Index of Mixtures.

[0191] The experimental results were calculated using DPS data processing software, and the toxicity regression equations and EC values ​​for single-dose and mixtures with different ratios of the test agents were obtained. 50 The co-toxicity coefficient (CTC) of the two agents in different ratios was calculated to screen for the optimal ratio of the test agents. All raw data from each treatment were recorded for all replicates.

[0192] Results of flufenoxadiazam and flutriafol assays against soybean rust virus:

[0193] Table 4. Combined toxicity determination of flufenoxadiazam and flutriafol against soybean rust.

[0194] flufenoxadiazam(A) 4.5627 45.657 - - Epoxiconazole (B) 2.0832 100.000 - - A:B = 1:10 1.6308 127.741 95.060 134.380 A:B = 1:8 1.5307 136.095 93.962 144.840 A:B = 1:4 1.4842 140.358 89.131 157.474 A:B = 1:3 1.4773 141.014 86.414 163.184 A:B = 5:1 2.0023 104.040 54.714 190.152 A:B = 8:1 2.2836 91.224 51.695 176.466 A:B = 10:1 2.5107 82.973 50.597 163.986

[0195] Table 4 shows that the combination of flufenoxadiazam and flutriafol has a good fungicidal effect on soybean rust. The combined toxicity assay results of different ratios of flufenoxadiazam and flutriafol against soybean rust show that flutriafol has higher toxicity against soybean rust, EC50.50 The EC50 concentration of flufenoxadiazam against soybean rust was 2.0832 mg / L. 50 The concentration was 4.5627 mg / L. Flufenoxadiazam and flutriafol showed a synergistic effect in the mass ratio range of 1:10 to 10:1, with the most significant synergistic effect observed at a mass ratio of 5:1, and a co-toxicity coefficient of 190.152.

[0196] Results of flufenoxadiazam and ipfentrifluconazole assays for soybean rust virus infectivity:

[0197] Table 5. Combined toxicity assay of flufenoxadiazam and ipfentrifluconazole against soybean rust.

[0198] flufenoxadiazam(A) 4.5627 72.933 - - ipfentrifluconazole(B) 3.3277 100.000 - - A:B = 1:10 2.6655 124.843 97.539 127.993 A:B = 1:8 2.2951 144.992 96.993 149.487 A:B = 1:4 2.1123 157.539 94.587 166.556 A:B = 1:2 1.9814 167.947 90.978 184.603 A:B = 2:1 2.3027 144.513 81.955 176.332 A:B = 4:1 2.8482 116.835 78.346 149.127 A:B = 10:1 3.2546 102.246 75.393 135.617

[0199] Table 5 shows that the combination of flufenoxadiazam and ipfentrifluconazole has a good fungicidal effect against soybean rust. The combined toxicity assay results of different ratios of flufenoxadiazam and ipfentrifluconazole against soybean rust show that ipfentrifluconazole has higher toxicity against soybean rust, EC50. 50 The concentration was 3.3277 mg / L. Flufenoxadiazam and ipfentrifluconazole showed synergistic effects in a mass ratio of 1:10 to 10:1, with the most significant synergistic effect observed at a mass ratio of 1:2, resulting in a co-toxicity coefficient of 184.603.

[0200] Results of flufenoxadiazam and pyraclostrobin assays against soybean rust virus:

[0201] Table 6. Combined toxicity test of flufenoxadiazam and pyraclostrobin against soybean rust.

[0202] flufenoxadiazam(A) 4.5627 2.115 - - Isopyrazam (B) 0.0965 100.000 - - A:B = 1:20 0.0781 123.560 95.339 129.600 A:B = 1:12 0.0664 145.331 92.470 157.165 A:B = 1:6 0.0634 152.208 86.016 176.952 A:B = 1:3 0.0749 128.838 75.529 170.582 A:B = 1:1 0.1165 82.833 51.057 162.234 A:B = 3:1 0.2277 42.380 26.586 159.407 A:B = 6:1 0.4383 22.017 16.099 136.763 A:B = 12:1 0.7770 12.420 9.645 128.772

[0203] Table 6 shows that the combination of flufenoxadiazam and pyraclostrobin has a good fungicidal effect against soybean rust. The combined toxicity test results of different ratios of flufenoxadiazam and pyraclostrobin against soybean rust showed that pyraclostrobin had higher toxicity against soybean rust, EC50. 50The concentration was 0.0965 mg / L. Flufenoxadiazam and pyraclostrobin showed synergistic effects in a mass ratio of 1:20 to 12:1, with the most significant synergistic effect observed at a mass ratio of 1:6, resulting in a co-toxicity coefficient of 176.952.

[0204] Field efficacy trials

[0205] Example 3: Field efficacy trial of flufenoxadiazam mixed with flutriafol or ipfentrifluconazole for controlling wheat leaf rust.

[0206] Experimental basis: Refer to "Guidelines for Field Efficacy Trials of Pesticides (I) Control of Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust) with Fungicides" (GB / T17980.23-2000)

[0207] Experiment location: Wheat field in Nanfan Village, Shouguang City, Shandong Province.

[0208] Experimental target: Puccinia recondita f.sp.tritici, the causal agent of wheat leaf rust.

[0209] Experimental crop, variety, and growth status: Wheat (Tanmai 98).

[0210] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 The cells are randomly arranged.

[0211] Application method: Use conventional spraying method to evenly spray the various treatment solutions on both sides of the leaves.

[0212] Investigation and statistical methods: Disease index was investigated before application of pesticide. Seven days after the first application and ten days after the second application, five representative sampling points were randomly selected from each plot. Twenty plants were investigated at each point, and the top three leaves of each plant were investigated. The disease was classified according to the percentage of diseased area on each leaf to the total leaf area.

[0213] Grading method:

[0214] Grade 0: No lesions;

[0215] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0216] Grade 3: The area of ​​lesions accounts for less than 6% to 25% of the total leaf area;

[0217] Level 5: The area of ​​lesions accounts for less than 26% to 50% of the total leaf area;

[0218] Level 7: The area of ​​lesions accounts for less than 51% to 75% of the total leaf area;

[0219] Level 9: The area of ​​diseased spots accounts for more than 76% of the total leaf area.

[0220] Methods for calculating drug efficacy:

[0221] Calculate the disease index and prevention efficacy using the following formula.

[0222]

[0223]

[0224] In the formula:

[0225] CK0—Disease index in the blank control area before drug administration;

[0226] CK1—Disease index after drug administration in the blank control area;

[0227] PTO—Disease index in the treatment area before application of pesticides;

[0228] PT1—Disease index after drug treatment in the treatment area.

[0229] Experimental results and analysis:

[0230] Table 7 Results of field plot trials of different pesticides for controlling wheat leaf rust

[0231]

[0232] As shown in Table 7, the control effects of different mixtures of flufenoxadiazam and flutriafol on wheat leaf rust were all superior to the control agent. Seven days after the first application, 24% flufenoxadiazam·flutriafol water-dispersible granules (5:1), 20% flufenoxadiazam·flutriafol suspension (1:1), and 16% flufenoxadiazam·flutriafol emulsifiable concentrate (3:1) all showed good rapid-acting properties. Ten days after the second application, the combination of 20% flufenoxadiazam·flutriafol suspension (1:1) showed the most outstanding control effect on wheat leaf rust, with a control efficacy of 89.09%.

[0233] Table 8 Results of field plot trials of different pesticides for controlling wheat leaf rust

[0234]

[0235] Note: The efficacy (%) in the table above is the average of each replicate.

[0236] As shown in Table 8, the control efficacy of different mixtures of flufenoxadiazam and ipfentrifluconazole against wheat leaf rust was superior to the control agent. Seven days after the first application and ten days after the second application, the combination of 20% flufenoxadiazam·ipfentrifluconazole suspension (3:1) showed the most significant control efficacy against wheat leaf rust, with control efficiencies of 82.52% and 88.45%, respectively.

[0237] Example 5: Field efficacy trial of flufenoxadiazam mixed with flutriafol or ipfentrifluconazole for controlling soybean rust.

[0238] Experimental basis: Refer to "Guidelines for Field Efficacy Testing of Pesticides (II) - Control of Soybean Rust with Fungicides (GB / T17980.89-2004)"

[0239] Experimental location: Soybean field in Gaomeng Village, Xintai City, Shandong Province. The soil layer of the experimental site is deep, and the water and fertilizer management is above average, which is in line with the local scientific agricultural practices.

[0240] Experimental target: Soybean rust fungus (Phakopsora pachyrhizi Syd.).

[0241] Experimental crop, variety, and growth status: Soybean (Liaodou 15).

[0242] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 .

[0243] Application method: Use conventional spraying method. Apply the first application at the initial stage of disease, and apply the next application after 7 days. Apply a total of 2 times. Use a backpack electric sprayer to evenly spray the agents of each treatment on the front and back of the leaves and branches, ensuring that the leaves are wet but not dripping.

[0244] Investigation and statistical methods: Disease index was investigated before application of pesticides and 10 days after the last application. During the investigation, five points were sampled diagonally in each plot, with 20 plants sampled at each point. Four leaves from the upper, middle and lower parts of each plant were sampled, and the total number of leaves and the number of diseased leaves at each level were recorded.

[0245] Grading method:

[0246] Grade 0: No lesions;

[0247] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0248] Grade 3: The area of ​​lesions accounts for less than 6% to 25% of the total leaf area;

[0249] Level 5: The area of ​​lesions accounts for less than 26% to 50% of the total leaf area;

[0250] Level 7: The area of ​​lesions accounts for less than 51% to 75% of the total leaf area;

[0251] Level 9: The area of ​​lesions accounts for more than 76% of the total leaf area.

[0252] Methods for calculating drug efficacy

[0253] Calculate the disease index and prevention efficacy using the following formula:

[0254]

[0255]

[0256] In the formula:

[0257] CK0—Disease index in the blank control area before drug administration;

[0258] CK1—Disease index after drug administration in the blank control area;

[0259] PTO—Disease index in the treatment area before application of pesticides;

[0260] PT1—Disease index after drug treatment in the treatment area.

[0261] Results and analysis of field efficacy trials of 30% flufenoxadiazam·flufenoxadiazam suspension for controlling soybean rust:

[0262] Table 9. Field efficacy trial results of 30% flufenoxadiazam·flufenoxatil suspension for controlling soybean rust.

[0263]

[0264] Note: The efficacy (%) in the table above is the average of each replicate.

[0265] As shown in Table 9, the experimental results indicate that 24% flufenoxadiazam·flufenoxam water-dispersible granules (5:1) exhibited good control efficacy against soybean rust. Ten days after application, apply 50 g / hm² of the active ingredient. 2 70g / hm 2 and 100g / hm 2 The average control efficacy of the three treatments against soybean rust was 84.76%, 87.34%, and 90.05%, respectively. The treatment with 100 g / ha of active ingredient showed the best control efficacy. This compound formulation showed better control efficacy at higher concentrations than at lower concentrations. The control agent, 30% flufenoxadiazam suspension, had an active ingredient dosage of 250 g / ha. 2125g / L flutriafol suspension concentrate, active ingredient dosage: 125g / hm 2 The preventive efficacy was 76.45% and 78.47%, respectively.

[0266] Results and analysis of field efficacy trials of 20% flufenoxadiazam·ipfentrifluconazole suspension for controlling soybean rust:

[0267] Table 10. Field efficacy trial results of 20% flufenoxadiazam·ipfentrifluconazole suspension for controlling soybean rust.

[0268]

[0269] Note: The efficacy (%) in the table above is the average of each replicate.

[0270] Table 10 shows that the experimental results indicate that 18% flufenoxadiazam·ipfentrifluconazole EC (1:2) exhibits good control efficacy against soybean rust. Ten days after application, apply 60 g / hm² of the active ingredient. 2 80g / hm 2 and 100g / hm 2 The average control efficacy of the three treatments against soybean rust was 84.54%, 88.57%, and 90.85%, respectively, based on an effective ingredient concentration of 100 g / hm². 2 The treatment showed the best control efficacy, with the compound formulation exhibiting superior control at high concentrations compared to low concentrations. The control agent, 30% flufenoxadiazam suspension, was used at an effective ingredient dosage of 250 g / hm². 2 25% ipfentrifluconazole suspension active ingredient dosage: 150 g / hm 2 The preventive efficacy was 76.45% and 79.43%, respectively.

[0271] Example 6: Field efficacy trial of flufenoxadiazam and pyraclostrobin for controlling wheat leaf rust

[0272] Experimental basis: Refer to GB / T 17980.23-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust) with Fungicides"

[0273] Experiment location: Wheat field in Zhenjiazhuang Village, Jimo District, Qingdao City, Shandong Province.

[0274] Experimental target: Puccinia recondita Rob. ex Desm. f. sp. tritici Erikss. et Henn., the fungus that causes wheat leaf rust.

[0275] Experimental crop and variety: Wheat (Shannong 27).

[0276] Experimental setup: Each experimental plot was arranged in a randomized block design, with a guard row surrounding each plot. Each treatment was replicated four times, with each plot measuring 20m. 2 .

[0277] Application method: Use conventional spraying method to evenly spray each treatment agent on both sides of the wheat leaves, ensuring uniform spraying. The amount of pesticide solution applied per acre is 50 kg.

[0278] Survey and statistical methods: The experiment was conducted 10 days after the last application of the drug. Five representative sampling points were randomly selected from each plot, with 20 plants sampled at each point. The top 3 leaves of each plant were sampled, and the plants were graded according to the percentage of disease spots on each leaf covering the entire leaf area.

[0279] Grading method:

[0280] Grade 0: No lesions;

[0281] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0282] Grade 3: The area of ​​lesions accounts for less than 6% to 25% of the total leaf area;

[0283] Level 5: The area of ​​lesions accounts for less than 26% to 50% of the total leaf area;

[0284] Level 7: The area of ​​lesions accounts for less than 51% to 75% of the total leaf area;

[0285] Level 9: The area of ​​diseased spots accounts for more than 76% of the total leaf area.

[0286] Methods for calculating drug efficacy

[0287] Calculate the disease index and prevention efficacy using the following formula:

[0288]

[0289]

[0290] Experimental results and analysis:

[0291] Table 11 Results of field plot trials of different pesticides for controlling wheat leaf rust

[0292]

[0293]

[0294] Note: The efficacy (%) in the table above is the average of each replicate.

[0295] As shown in Table 11, the control effects of different mixtures of flufenoxadiazam and pyraclostrobin on wheat leaf rust were all superior to the control agent. Ten days after the second application, the combination of 22% flufenoxadiazam·pyraclostrobin suspension (1:1) showed the most outstanding control effect on wheat leaf rust, with a control efficacy of 90.93%.

[0296] Example 7: Field efficacy trial of flufenoxadiazam and pyraclostrobin for controlling soybean rust

[0297] Test basis: Refer to GB / T 17980.89-2004 "Field Efficacy Test Guidelines for Pesticides (II) Control of Soybean Rust with Fungicides".

[0298] Experiment location: Soybean field in Dazhouzhuang Village, Yucheng, Shandong Province.

[0299] Experimental target: Soybean rust fungus (Phakopsora pachyrhizi Syd.).

[0300] Experimental crop and variety: soybean.

[0301] Experimental setup: Each cell was arranged in a randomized block design, with a guard row around each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 .

[0302] Application method: Use conventional spraying method to apply the pesticide at the early stage of soybean rust disease, and spray it evenly on both sides of the leaves.

[0303] Survey and statistical methods: The survey was conducted 10 days after the last application of the pesticide. Five representative diagonal sampling points were randomly selected from each plot. 20 plants were surveyed at each point, and 4 leaves from the upper, middle and lower parts of each plant were surveyed. The total number of leaves and the number of diseased leaves at each level were recorded.

[0304] Grading method:

[0305] Grade 0: No lesions;

[0306] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0307] Grade 3: The area of ​​lesions accounts for less than 6% to 25% of the total leaf area;

[0308] Level 5: The area of ​​lesions accounts for less than 26% to 50% of the total leaf area;

[0309] Level 7: The area of ​​lesions accounts for less than 51% to 75% of the total leaf area;

[0310] Level 9: The area of ​​lesions accounts for more than 76% of the total leaf area.

[0311] Methods for calculating drug efficacy

[0312] Calculate the disease index and prevention efficacy using the following formula.

[0313]

[0314]

[0315] Table 12. Field efficacy trial results of 20% flufenoxadiazam·pyraclostrobin suspension for controlling soybean rust.

[0316]

[0317] Note: The efficacy (%) in the table above is the average of each replicate.

[0318] Table 12 shows that the experimental results indicate that 28% flufenoxadiazam·pyraclostrobin suspension (1:6) exhibits good control efficacy against soybean rust. Ten days after the last application, 75 g / hm² of the active ingredient of 28% flufenoxadiazam·pyraclostrobin suspension (1:6) was applied. 2 100g / hm 2 and 125g / hm 2 The average control efficacy of the three treatments against soybean rust was 82.20%, 85.09%, and 88.40%, respectively. The treatment with 125 g / ha of active ingredient showed the best control efficacy. This compound formulation showed better control efficacy at higher concentrations than at lower concentrations. The control agent, 30% flufenoxadiazam suspension, had an active ingredient dosage of 250 g / ha. 2 125g / L pyraclostrobin suspension concentrate, active ingredient dosage 100g / hm 2 The preventive efficacy was 72.20% and 76.90%, respectively.

[0319] Example 8: Field efficacy trial of flufenoxadiazam and pyraclostrobin for controlling corn rust

[0320] Experiment location: Cornfield in Zhangjiazhuang Village, Laixi City, Qingdao, Shandong Province.

[0321] Experimental target: Puccinia sorghi Schw, the causal agent of corn rust.

[0322] Experimental crop and variety: Maize (Zhengdan 958)

[0323] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 The cells are randomly arranged.

[0324] Application method: Use a Guardian WS-16 manual sprayer to spray the entire plant evenly. The application time is June 15, 2020. The weather was cloudy on the day of application.

[0325] Survey and statistical methods: The survey was conducted 10 days after the last application of the pesticide. Five representative points or five diagonal points were randomly selected from each plot. Twenty plants were surveyed at each point. One leaf from the upper, middle and lower parts of each plant was selected for the survey. The classification was based on the percentage of disease spots on each leaf covering the entire leaf area.

[0326] Grading method:

[0327] Grade 0: No lesions;

[0328] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0329] Grade 3: The area of ​​lesions accounts for less than 6% to 25% of the total leaf area;

[0330] Level 5: The area of ​​lesions accounts for less than 26% to 50% of the total leaf area;

[0331] Level 7: The area of ​​lesions accounts for less than 51% to 75% of the total leaf area;

[0332] Level 9: The area of ​​lesions accounts for more than 76% of the total leaf area.

[0333] Methods for calculating drug efficacy

[0334] Calculate the disease index and prevention efficacy using the following formula.

[0335]

[0336]

[0337] Table 13 Results of field plot trials of 20% flufenoxadiazam·pyraclostrobin suspension for controlling corn rust.

[0338]

[0339] Note: The efficacy (%) in the table above is the average of each replicate.

[0340] As shown in Table 13, the control efficacy of different mixtures of flufenoxadiazam and pyraclostrobin against corn rust was superior to the control agent. Ten days after the last application, 80 g / hm² of 22% flufenoxadiazam·pyraclostrobin suspension concentrate (1:1) was applied. 2 120g / hm 2 and 160g / hm 2The average control efficacy of the three treatments against corn rust was 87.97%, 89.30%, and 91.49%, respectively. The treatment with 160 g / ha of active ingredient showed the best control efficacy. This compound formulation showed better control efficacy at higher concentrations than at lower concentrations. The control agent, 30% flufenoxadiazam suspension, had an active ingredient dosage of 265 g / ha. 2 125g / L pyraclostrobin suspension concentrate, active ingredient dosage 110g / hm 2 The preventive efficacy was 79.52% and 81.80%, respectively.

[0341] Through indoor toxicity testing and field trials, the pesticide composition of flufenoxadiazam described in this invention, compounded with any one of flutriafol, ipfentrifluconazole, or pyraclostrobin, showed good control efficacy against plant rust diseases, particularly wheat leaf rust, soybean rust, and corn rust. The pesticide composition or formulation obtained by this invention exhibits significant control efficacy, superior to single agents in delaying the development of resistance and prolonging residual effect. Furthermore, no phytotoxicity was observed in the trials, indicating that the improved synergistic effect of the resulting pesticide composition or formulation reduces production and usage costs while ensuring crop safety.

[0342] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. The use of a pesticide composition containing flufenoxadiazam for the control of rust fungal diseases, characterized in that, The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is flufenoxadiazam and active ingredient B is ipfentrifluconazole, the mass ratio of active ingredient A to active ingredient B is 1:25 to 20:1, and the disease is wheat rust and bean rust.

2. The use according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:10 to 10:

1.

3. The use according to claim 1, characterized in that, The total weight of the pesticide composition is 100% wt, and the total weight of active ingredient A and active ingredient B accounts for 1% to 80% of the total weight of the pesticide composition.

4. The use according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

5. The use according to claim 1, characterized in that, The pesticide composition is prepared into any agriculturally permissible formulation, wherein the formulation is selected from solid or liquid formulations; The liquid formulation is selected from emulsifiable concentrates, suspensions, or microemulsions, and the solid formulation is selected from water-dispersible granules or wettable powders.

6. The use according to claim 1, characterized in that, The pesticide composition is applied at an effective dose to the pathogen or the medium in which it occurs or its growth environment.

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