A plant-derived composition having a cooling, soothing effect and uses thereof

Abstract

The application provides a composition with cooling and soothing effects and application thereof. The composition comprises shuixiandan and pine red plum extract. The application first discovers that shuixiandan and pine red plum extract with soothing effects can obtain cooling and temperature-reducing effects whether used alone or combined together. Experimental results show that shuixiandan and pine red plum extract combination can obtain the best cooling and temperature-reducing effect, and the combination has synergistic effects. Moreover, both are plant-derived components, and traditional mint and other cooling agents do not need to be added, and are especially suitable for the mild cooling and temperature-reducing needs of sensitive skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to a composition having cooling and soothing effects and its application. Background Technology

[0002] Sensitive skin clinically manifests as subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, or psychological factors. These symptoms may or may not be accompanied by objective signs such as erythema, scaling, and telangiectasia. Symptoms can last for hours or days and the skin typically cannot tolerate ordinary cosmetics. In recent years, the market share of skincare products designed to soothe sensitive skin has been steadily increasing, especially for symptoms such as burning, itching, and sunburn. Consumers not only expect more effective soothing products but also those with calming effects, providing a cooling and comfortable feeling to the skin.

[0003] Existing technologies have reported compositions with soothing and cooling effects. For example, CN115645311A discloses an oil composition with repairing and cooling effects and its application. This oil composition includes meadowfoam seed oil, sea buckthorn fruit oil, squalane, stearyl heptaester, phytosterol low-erucic acid rapeseed oil glycerides, and jojoba oil. It has excellent moisturizing, cooling, anti-allergic, redness-reducing, and skin-nourishing and repairing effects, and is gentle and non-irritating. Among them, stearyl heptaester, phytosterol low-erucic acid rapeseed oil glycerides, and jojoba oil have potential interactions, positively influencing each other and synergistically promoting the effects.

[0004] Existing soothing ingredient compositions rarely also possess a cooling effect, and ingredients with significant cooling effects are often irritating and unsuitable for sensitive skin. For example, erythritol has good endothermic properties when dissolved (heat of solution is -180 J / g), and can be used in cosmetic formulations as a moisturizer and cooling agent. However, due to its inherent properties, erythritol requires a high concentration in the formulation to produce a cooling sensation during application. While erythritol can enhance the moisturizing effect of cosmetics, its multi-hydroxyl structure makes the formulation relatively sticky, reducing the user experience.

[0005] Ethanol is a commonly used solvent in cosmetic development. It is miscible with water in any proportion and evaporates on the skin's surface, carrying away heat and producing a cooling sensation. However, due to its small molecular weight, ethanol has excellent skin permeability, and high concentrations can denature proteins. Products containing ethanol are only suitable for skin with good tolerance to ethanol. Long-term use on otherwise healthy skin can easily lead to dryness and roughness. Large amounts of ethanol in products may cause itching, allergic reactions, and other symptoms.

[0006] When solid oils are applied to the skin, they melt and absorb some heat as they transform from a solid to a liquid state. The amount of heat absorbed under varying temperatures is related to the oil's specific heat; absorbing more heat results in a greater cooling sensation. Adding a suitable amount of solid oil to an emulsified formula can create a melting and cooling sensation during application. However, using solid oils as a means of generating a cooling sensation has many limitations in product development. Solid oils, due to their large molecular weight, often contribute to a sticky feel in the formula and can only be used in emulsified systems, not transparent ones. Furthermore, the heat absorbed during the melting process cannot provide a sustained cooling sensation; it only produces a momentary, slight cooling effect during application.

[0007] Menthol is a terpene compound and the main component of peppermint and spearmint essential oils. Peppermint and its derivatives are characterized by their rapid cooling effect, refreshing aroma, and pleasant taste. However, when used in large quantities, peppermint and its derivatives can have a bitter taste, are highly volatile, and have a short-lasting cooling effect, which can easily cause irritation.

[0008] Therefore, existing cooling agents generally suffer from insufficiently long-lasting cooling effects, extremely poor solubility, and defects such as crystallization and water-oil separation when applied to aqueous products, leading to unstable product quality. Alternatively, they may require excessive raw materials, resulting in high processing costs. Therefore, developing more products with significant cooling and soothing effects is essential. Summary of the Invention

[0009] In view of this, the technical problem to be solved by the present invention is to provide a composition with cooling and soothing effects and its application, which has a significant cooling effect and a long-lasting cooling sensation.

[0010] One of the objectives of this invention is to provide a composition having cooling and soothing effects, comprising Shuyidan and pine berry extract.

[0011] One of the raw materials of this invention, Shuyidan, contains the following active ingredients: Sophora flavescens root extract, the key active ingredient of which is oxymatrine; Tribulus terrestris fruit extract, the key active ingredient of which is tribulus saponin; Cornus officinalis fruit extract, the key active ingredient of which is ursolic acid; while Sophora flavescens root mainly targets calcium ion channels and the PI3K signaling pathway, and Sophora flavescens root extract has the ability to inhibit calcium ion channels and PI3K signaling pathway. 2+ The potential of Tribulus terrestris fruit extract to inhibit TNF-α release is explored. This raw material is produced by Dongfang Miaosen Company and its trade name is Sensysoothe.

[0012] Another raw material of the present invention, Manuka extract, refers to a complex composed of butylene glycol, NELUMBIUM SPECIOSUM flower water, LEPTOSPERMUM SCOPARIUM leaf extract, and STEPHANIA TETRANDRA extract. The composition is a raw material produced by Jianeng Biotechnology, with the commercial name Manuka Complex Extract.

[0013] It is found in cell experiments that this raw material has an inhibitory effect on the production of NO and TRPV1, significantly reduces the stinging sensation caused by capsaicin, and has a strong inhibitory effect on skin stinging, erythema and telangiectasia caused by external irritants and inflammation; in addition, it can reduce the redness and swelling caused by UV, has a significant repair function for UV-induced inflammation, reduces redness and swelling caused by UV and chronic inflammation, and reduces cell damage caused by irritation. It is found in human efficacy tests that after the raw material is used for 1 to 4 weeks, skin lesions and redness are improved, indicating that the raw material can effectively repair skin damage, improve redness, and exert a soothing effect.

[0014] The present invention first discovers that the combination of the soothing active ingredient Shuyudan and Manuka extract can achieve a cooling effect. The experimental results show that the combination of Shuyudan and Manuka extract can obtain the optimal cooling effect, and the combination of the two has a synergistic effect. Moreover, both are plant-derived ingredients, no traditional cooling agents such as menthol need to be added, and they are suitable for the mild cooling needs of sensitive skin.

[0015] Preferably, the weight ratio of Shuyudan to Manuka extract is 100:1 to 1:100, more preferably 2:1 to 1:2.

[0016] Further preferably, the weight ratio of Shuyudan to Manuka extract is 1:1.

[0017] The experimental results show that when the weight ratio of Shuyudan to Manuka extract is 1:1, the optimal physical cooling and soothing effects are achieved.

[0018] In the present invention, the active ingredients of Shuyudan include: Sophora flavescens root extract, Tribulus terrestris fruit extract, and Cornus officinalis fruit extract.

[0019] Optionally, the solvent in Shuyudan is butylene glycol.

[0020] The active ingredients of the Manuka extract include: lotus flower water, LEPTOSPERMUM SCOPARIUM leaf extract, and STEPHANIA TETRANDRA extract.

[0021] Optionally, the solvent in the Manuka extract is butylene glycol.

[0022] Another object of the present invention is to provide the use of the above-mentioned composition having cooling and soothing effects in the preparation of cosmetics having cooling and soothing effects.

[0023] Cosmetics containing the above composition provide a gentle cooling sensation during use. Skin surface temperature measurements after use show a significant cooling effect. The composition also has a synergistic soothing effect on the skin, with a long-lasting cooling sensation. Cosmetic formulas containing this composition are stable over long periods and can effectively relieve discomfort caused by skin redness and sensitivity, making them suitable for sensitive skin.

[0024] The cosmetics mentioned are preferably skin-use cosmetics.

[0025] The present invention does not specifically limit the types of cosmetics mentioned above, and can be any cosmetic well known in the art that needs to have cooling and soothing effects, and its dosage form includes, but is not limited to, water, lotion, cream and other dosage forms.

[0026] In some specific embodiments of the present invention, the cosmetics include, but are not limited to, one or more of the following: skin care gel, face mask, skin care lotion, face cream, etc.

[0027] Another object of the present invention is to provide a skin care gel with cooling and soothing effects, comprising the above-mentioned composition with cooling and soothing effects.

[0028] In the skin care gel provided by the present invention, the composition accounts for 1% to 5% of the weight percentage of the product, preferably 3%.

[0029] Preferably, the skin-care gel with cooling and soothing effects comprises the following components:

[0030] Shuyidan, Pine Red Plum Extract, Polyol, Acryloyldimethyltaurate ammonium / VP copolymer, Carbomer 980, 1,2-Hexanediol, p-hydroxyacetophenone, Arginine, Water.

[0031] In some specific embodiments of the present invention, the skin care gel with cooling and soothing effects comprises the following components by weight percentage:

[0032]

[0033] The polyol is preferably glycerol and butanediol.

[0034] In some specific embodiments of the present invention, the skin care gel with cooling and soothing effects comprises the following components by weight percentage:

[0035]

[0036]

[0037] Another object of the present invention is to provide a method for repairing skin, comprising: applying the above-mentioned composition having cooling and soothing effects or the above-mentioned skin care gel having cooling and soothing effects to the skin surface.

[0038] The above applications can specifically be applied as a poultice or a coating.

[0039] Compared with the prior art, the present invention provides a composition with cooling and soothing effects, comprising Shuyidan and pine berry extract.

[0040] Compared with the prior art, the present invention has at least the following beneficial effects:

[0041] 1. This invention is the first to combine Shuyidan and pine syrup extract, which has a synergistic soothing and cooling effect. It can significantly reduce the skin surface temperature, and can reduce it by more than 3% immediately, thus achieving a good cooling and soothing effect.

[0042] 2. All raw materials in the composition are plant extracts, which are highly safe and have low irritation. Compared with traditional cooling agents, they are gentler. While soothing the skin, they can also play a good role in cooling and calming, and are more suitable for sensitive skin.

[0043] 3. The compounded composition has high versatility and good compatibility. It can be dissolved in aqueous systems without the need to add chemical cooling agents (such as menthol and its derivatives), high content emulsifiers, solubilizers or ethanol for auxiliary dissolution, thus reducing damage to the skin.

[0044] 4. The composition has good stability and will not cause precipitation or water-oil separation when added to a cosmetic system;

[0045] 5. This invention is the first to discover that soothing ingredients such as Shuyidan and Songhongmei have a significant effect on reducing skin surface temperature. Detailed Implementation

[0046] To further illustrate the present invention, the following detailed description of the composition with cooling and soothing effects and its applications, in conjunction with embodiments, is provided by the present invention.

[0047] Examples 1-5: Efficacy Verification of the Combination of Shuyidan and Pine Red Plum Extract

[0048] Gel samples were prepared according to the proportions in Table 1. The preparation process is as follows:

[0049] ① Preparation of gel base material: Based on 100g, weigh 4g glycerol, 0.4g ammonium acryloyldimethyl taurate / VP copolymer, 5g butanediol, 0.2g carbomer 980, 0.6g 1,2-hexanediol, and 0.5g p-hydroxyacetophenone. Stir and heat until dissolved and uniform. Cool to about 65℃ and add 0.2g arginine dissolved in deionized water. Stir and disperse evenly. Cool to about 45℃ to obtain the gel base material.

[0050] ② Take a certain amount of gel base material and add it to the cooling ingredient combination shown in Table 1, and add deionized water to 100%. Stir at a speed of about 900 rpm for 10 minutes to obtain a soothing gel sample.

[0051] Table 1. Combination and content (wt%) of cooling ingredients in Examples 1-5

[0052] Shuyidan 0％ 3％ 1％ 2％ 1.5％ Pineberry extract 3％ 0％ 2％ 1％ 1.5％

[0053] The cooling sensation was rated, and the results are shown in Table 2.

[0054] The rating criteria for the intensity of cooling sensation are as follows:

[0055] Consumers participating in the test made subjective evaluations, scoring the cooling sensation they felt from weak to strong on a scale of 0 to 5. The average score of all consumers' scores was taken as the comprehensive score for each embodiment.

[0056] The calculation standard for the duration of the cooling sensation is as follows:

[0057] Consumers participating in the test made subjective evaluations. The timing started from when the cooling sensation was felt after applying the sample and ended when the cooling sensation was rated as 0. The time (min) obtained was the duration of the cooling sensation of the test sample. The average of the durations of each consumer was taken as the comprehensive score of each embodiment.

[0058] Table 2 Cooling sensation rating results

[0059]

[0060]

[0061] In terms of cooling intensity, the results were: Example 5 > Example 3 > Example 4 > Example 2 > Example 1.

[0062] In terms of perceived duration of coolness: Example 5 > Example 4 > Example 1 > Example 3 > Example 2.

[0063] Experimental conclusion:

[0064] The optimal ratio of Shuyidan and Songhongmei extract was found to be 1:1 (by mass).

[0065] In terms of the duration of the cooling sensation, the 1:1 (by weight) ratio of Shu Yi Dan and Song Hong Mei extract is superior to other combinations, with the longest duration.

[0066] The temperature change after using the gel sample was tested using an infrared thermometer, and the results are shown in Table 6.

[0067] The specific testing method is as follows:

[0068] 1. First, measure a 2cm x 2cm area on the arm and use an infrared thermometer to test the initial temperature;

[0069] 2. Apply 2g of test sample evenly to the area, wait 3 minutes, and then test the temperature again with an infrared thermometer. Take the average of three measurements as the average temperature after use.

[0070] Comparative Examples 1-8

[0071] Gel samples were prepared according to the proportions in Table 3. The preparation process is as follows:

[0072] ① Preparation of gel base material: Based on 100g, weigh 4g glycerol, 0.4g ammonium acryloyldimethyl taurate / VP copolymer, 5g butanediol, 0.2g carbomer 980, 0.6g 1,2-hexanediol, and 0.5g p-hydroxyacetophenone. Stir and heat until dissolved and uniform. Cool to about 65℃ and add 0.2g arginine dissolved in deionized water. Stir and disperse evenly. Cool to about 45℃ to obtain the gel base material.

[0073] ② Take out a certain amount of gel base material and add the cooling ingredients shown in Table 3, and add deionized water to 100%. Stir at about 900 rpm for 10 minutes to obtain a soothing gel sample.

[0074] The cooling sensation was rated, and the results are shown in Table 5.

[0075] Table 3. Cooling ingredients and their content in Comparative Examples 1-8

[0076]

[0077]

[0078] Note: The above addition amount is selected based on the characteristics of the raw materials and added according to the normal effective amount.

[0079] Comparative Examples 9-14

[0080] Gel samples were prepared according to the proportions in Table 4. The preparation process is as follows:

[0081] ① Preparation of gel base material: Based on 100g, weigh 4g glycerol, 0.4g ammonium acryloyldimethyl taurate / VP copolymer, 5g butanediol, 0.2g carbomer 980, 0.6g 1,2-hexanediol, and 0.5g p-hydroxyacetophenone. Stir and heat until dissolved and uniform. Cool to about 65℃ and add 0.2g arginine dissolved in deionized water. Stir and disperse evenly. Cool to about 45℃ to obtain the gel base material.

[0082] ② Take a certain amount of gel base material and add it to the cooling ingredient combination shown in Table 4. Add deionized water to bring the volume to 100%. Stir at approximately 900 rpm for 10 minutes to obtain a soothing gel sample.

[0083] The temperature change after using the gel sample was tested using an infrared thermometer, and the results are shown in Table 6.

[0084] The testing method is the same as in Examples 1-5.

[0085] Table 4 Comparative Examples 9-14: Combination and Content of Cooling Ingredients (wt%)

[0086] 9 blank 10 1% Royal Jelly + 1% Shuyidan 11 1% royal jelly + 5% gentian root 12 1% Royal Jelly + 2% Pineapple 13 5% gentian root + 1% Shuyidan 14 5% gentian root + 2% pine needle plum

[0087] Experimental results:

[0088] Table 5 Cooling sensation rating results

[0089]

[0090] Table 6 Temperature variations when using a combination of cooling ingredients

[0091]

[0092] Experimental conclusion:

[0093] Through subjective evaluation of the cooling effect by 10 testers and testing of skin surface temperature changes with an infrared thermometer, it was found that when added alone, gentian root and royal jelly had the best cooling effect. However, the combination of gentian root and royal jelly was not the combination with the greatest temperature difference. In the end, the combination of Shuyidan and pine saffron extract had the greatest temperature difference. It can be seen that the combination of Shuyidan and pine saffron extract has a synergistic effect on the cooling and soothing effects.

[0094] Example 6: Zebrafish Validation Experiment

[0095] The spin rate of pine syrup and shuyidan in zebrafish embryos was tested to characterize the soothing efficiency of the raw materials and their combinations. The individual raw materials and the combination of raw materials were tested to verify whether they had a synergistic effect. The specific experimental scheme is shown in Table 7 below:

[0096] Table 7 Zebrafish Validation Experiment Protocol

[0097]

[0098] The experimental steps are as follows:

[0099] ① Preparation of gel base material: Based on 100g, weigh 4g glycerol, 0.4g ammonium acryloyldimethyl taurate / VP copolymer, 5g butanediol, 0.2g carbomer 980, 0.6g 1,2-hexanediol, and 0.5g p-hydroxyacetophenone. Stir and heat until dissolved and uniform. Cool to about 65℃ and add 0.2g arginine dissolved in deionized water. Stir and disperse evenly. Cool to about 45℃ to obtain the gel base material.

[0100] ② Take out a certain amount of gel base material and add it to the cooling raw material composition shown in Table 7, and add deionized water to 100%. Stir at a speed of about 900 rpm for 10 minutes.

[0101] ③ After repackaging, a third-party zebrafish test was conducted.

[0102] The zebrafish test procedure is as follows:

[0103] 1. Prepare a stock solution by dissolving the embryo in fish embryo culture medium or methanol, and then dilute it to the experimental group concentration using fish embryo culture medium (final methanol volume fraction: 1%). Randomly select healthy, uniformly developed zebrafish embryos at 24 hpf and place them in 96-well plates, 5 embryos per well. Add 200 μL of the above-mentioned concentration of sample diluent to each well, with 3 replicates for each concentration. Incubate at 28 ± 0.5 for 60 min. A blank control group (treated with fish embryo culture medium) is also set up.

[0104] 2. After treatment, the number of spin movements of zebrafish embryos was recorded within 30 seconds using an inverted microscope. The concentration groups in which zebrafish embryos did not die and whose number of spin movements did not increase significantly compared with the blank control group were determined as the no-observable-effect concentrations (NOECs) of the samples.

[0105] 3. Serial dilutions were used to establish sample concentration groups within the NOEC concentration range. Healthy, uniformly sized zebrafish embryos (24 hpf) were randomly selected and placed in 96-well plates, 5 embryos per well. 200 μL of the sample concentration solution was added to each well, with 3 replicates per concentration. The treatment lasted 1 hour, with a blank control group included. After treatment, the solution in the 96-well plates was removed, and the samples were diluted with SDS to obtain a sample-SDS mixture (final SDS concentration 250 μmol / L). This mixture was treated for 15 minutes, and then the number of spin movements of the zebrafish embryos was recorded over 30 seconds using an inverted microscope.

[0106] 4. GraphPad Prism 8.0 software was used for data statistical analysis and graph plotting. One-way ANOVA analysis was used to analyze differences between groups, and P < 0.05 was considered statistically significant.

[0107] Experimental Results: The test results of zebrafish samples are shown in Table 8. Both raw materials, Shuyidan and Songhongmei, have a soothing effect on stinging. The combination of the two raw materials has a better soothing effect on the zebrafish than either raw material alone.

[0108] Table 8. Zebrafish Test Results

[0109]

[0110] Experimental conclusion:

[0111] The combination of Shuyidan and Songhongmei showed better soothing effects in the soothing test than the individual ingredients, indicating that the combination has a better soothing effect.

[0112] 1. By comparing the experimental results of the blank control group and groups A and B, it was found that the raw materials in experimental groups A and B had a soothing effect;

[0113] 2. A comparison of the experimental results of groups A, B, and C revealed that the combination of Shuyidan and Songhongmei extract had a better soothing effect than either of them alone.

[0114] The above description of the embodiments is only for the purpose of helping to understand the method and core ideas of the present invention. It should be noted that those skilled in the art can make several improvements and modifications to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A composition having a cooling and soothing effect, characterized in that, Including Shuyidan and Songhongmei extract; The weight ratio of Shuyidan and Songhongmei extract is 2:1 to 1:2; The active ingredients of Shuyidan include: Sophora flavescens root extract, Tribulus terrestris fruit extract, and Cornus officinalis fruit extract; The active ingredients of the pine bud extract include: lotus water, broom leaf Australian tea extract, and fangchi extract. The cooling and soothing effects include lowering the skin's surface temperature.

2. The composition with cooling and soothing effects according to claim 1, characterized in that, The weight ratio of Shuyidan and Songhongmei extract is 1:

1.

3. The use of the composition having cooling and soothing effects according to any one of claims 1 to 2 in the preparation of cosmetics having cooling and soothing effects.

4. The application according to claim 3, characterized in that, The cosmetics include one or more of the following: skin care gel, face mask, skin care lotion, and face cream.

5. A skin care gel with cooling and soothing effects, characterized in that, The composition having cooling and soothing effects as described in any one of claims 1 to 2.

6. The skin care gel with cooling and soothing effects according to claim 5, characterized in that, Includes the following components: Shuyidan, Pine Red Plum Extract, Polyol, Acryloyldimethyltaurate ammonium / VP copolymer, Carbomer 980, 1,2-hexanediol, p-hydroxyacetophenone, Arginine, Water.

7. The skin care gel with cooling and soothing effects according to claim 6, characterized in that, By weight percentage, it includes the following components: Shuyidan 0.5%-5%; Pine-leaf extract 0.2%-3%; Polyols 1%-20%; Acryloyldimethyl taurate ammonium / VP copolymer 0.1%-1%; Carbomer 980 0.1%-1%; 1,2-Hexanediol 0.1%-1%; p-Hydroxyacetophenone 0.1%-1%; Arginine 0.1%-1%; The remaining water.

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