Multi-channel rapid enrichment equipment for viruses in water body based on ultrafiltration method

Abstract

The application relates to a virus multi-channel rapid enrichment equipment in a water body based on an ultrafiltration method. The equipment comprises a plurality of enrichment devices and a waste liquid collecting device, the liquid outlet of each enrichment device is communicated with the liquid inlet of the waste liquid collecting device; the enrichment device comprises a liquid storage tank, a conveying pipeline and a filter assembly, the liquid storage tank has a storage cavity for storing a sample to be enriched, the storage cavity has an inlet and an outlet, the conveying pipeline has an air inlet and an air outlet, the conveying pipeline is used for conveying high-pressure gas, the air outlet is communicated with the inlet of the liquid storage tank, and the filter assembly comprises an ultrafiltration membrane corresponding to the outlet of the liquid storage tank. In the application, a plurality of enrichment devices are arranged, the filtration area is increased, the enrichment efficiency of the sample to be enriched on the ultrafiltration membrane is improved, in addition, the conveying pipeline is arranged, the conveying pipeline can convey high-pressure gas into the liquid storage tank through the inlet, that is, the sample to be enriched in the liquid storage tank is rapidly passed through the ultrafiltration membrane in a pressurized manner, and rapid enrichment is realized.

Description

Technical Field

[0001] This invention relates to the field of virus detection technology in water bodies, and in particular to a multi-channel rapid enrichment device for viruses in water bodies based on ultrafiltration. Background Technology

[0002] Because the concentration of viruses in wastewater is low and the wastewater samples are highly heterogeneous, direct measurement of virus concentration is quite difficult. Therefore, it is often necessary to enrich the wastewater samples first. However, the enrichment efficiency of existing enrichment equipment is still relatively low. Summary of the Invention

[0003] Therefore, it is necessary to provide a rapid multi-channel enrichment device for viruses in water based on ultrafiltration, addressing the technical problem of low enrichment efficiency in existing enrichment equipment.

[0004] A multi-channel rapid enrichment device for viruses in water based on ultrafiltration includes a frame, multiple enrichment devices, and a waste liquid collection device. Each enrichment device is connected to the frame, and the outlet of each enrichment device is connected to the inlet of the waste liquid collection device. Each enrichment device includes a storage tank, a delivery pipeline, and a filtration assembly. The storage tank has a storage cavity for storing samples to be enriched, and the storage cavity has an inlet and an outlet. The delivery pipeline has an air inlet and an air outlet for delivering high-pressure gas. The air outlet is connected to the inlet of the storage tank. The filtration assembly includes an ultrafiltration membrane, which corresponds to the outlet of the storage tank, so that the samples to be enriched flowing out of the outlet of the storage tank are filtered through the ultrafiltration membrane to form a contamination layer on the ultrafiltration membrane.

[0005] In one embodiment, the filtration assembly further includes a chuck and a sealing gasket. The chuck has a receiving cavity, and a first opening communicating with the outlet is provided on a first cavity wall of the receiving cavity. The sealing gasket and the ultrafiltration membrane are housed in the receiving cavity, and the sealing gasket is located between the ultrafiltration membrane and the first cavity wall to seal the gap between the first opening and the ultrafiltration membrane.

[0006] In one embodiment, the receiving cavity has a second opening on the second cavity wall opposite to the first cavity wall. The second opening is configured as the liquid outlet. The filtration assembly also includes a support plate housed in the receiving cavity. The support plate has through holes and is located between the second cavity wall and the ultrafiltration membrane. The support plate is used to support the ultrafiltration membrane.

[0007] In one embodiment, the chuck includes a first substrate and a second substrate, one of which has a groove and the other has a boss. The first substrate and the second substrate are inserted and engaged with the groove through the boss to form the receiving cavity.

[0008] In one embodiment, the first substrate is provided with a first U-shaped groove, and the groove wall of the first U-shaped groove is configured as the first cavity wall.

[0009] In one embodiment, the filter assembly further includes a fastener comprising a first retaining ring, a second retaining ring, and a fastening portion. The first retaining ring and the second retaining ring define a limiting hole for the chuck to pass through. The first retaining ring and the second retaining ring are connected by the fastening portion to abut against the first substrate and the second substrate to limit the first substrate and the second substrate from moving away from each other.

[0010] In one embodiment, the liquid storage tank includes a bottle body and a bottle cap, the bottle cap being threadedly connected to the bottle body, the storage cavity being disposed in the bottle body, the outlet being disposed on the bottle body, the inlet being disposed on the bottle cap, and a first aerosol filter covering the inlet being installed on the bottle cap.

[0011] In one embodiment, the bottle cap is provided with a quick-connect male connector, and the delivery pipeline is provided with a quick-connect female connector, the quick-connect female connector engaging with the quick-connect male connector.

[0012] In one embodiment, the enrichment device further includes a pressurization component, which includes an air pump, and the air inlet of the delivery pipeline is connected to the corresponding output port of the air pump.

[0013] The delivery pipeline is equipped with a pressure regulating valve for adjusting the pressure of the gas output from the corresponding air pump; and / or, the delivery pipeline is equipped with a pressure sensor for detecting the pressure of the gas delivered in the corresponding delivery pipeline.

[0014] In one embodiment, the waste liquid collection device includes a collection bottle and a vacuum pump. The inlet is located on the collection bottle, and the collection bottle is also provided with an air intake that communicates with the output port of the vacuum pump. A second anti-aerosolization filter is installed between the collection bottle and the vacuum pump, covering the air intake.

[0015] Beneficial effects:

[0016] The present invention provides a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, comprising a frame, multiple enrichment devices, and a waste liquid collection device. Each enrichment device is connected to the frame, and the outlet of each enrichment device is connected to the inlet of the waste liquid collection device. The enrichment device includes a storage tank, a delivery pipeline, and a filter assembly. The storage tank has a storage cavity for storing samples to be enriched, and the storage cavity has an inlet and an outlet. The delivery pipeline has an air inlet and an air outlet, and the delivery pipeline is used to deliver high-pressure gas. The air outlet is connected to the inlet of the storage tank. The filter assembly includes an ultrafiltration membrane, which corresponds to the outlet of the storage tank, so that the samples to be enriched flowing out of the outlet of the storage tank are filtered by the ultrafiltration membrane to form a contamination layer on the ultrafiltration membrane. This application increases the filtration area by setting up multiple enrichment devices, thereby improving the enrichment efficiency of the sample on the ultrafiltration membrane. In addition, the setting of the delivery pipeline allows high-pressure gas to be delivered into the storage tank through the inlet, that is, the sample to be enriched in the storage tank is rapidly passed through the ultrafiltration membrane by pressurization, achieving rapid enrichment. Compared with the existing technology of using a gas pump to draw in the sample, the pressure of the high-pressure gas delivered by the delivery pipeline is not limited. While being compatible with multiple enrichment devices, it can also adjust the enrichment speed of the sample on the ultrafiltration membrane, thus improving the performance of the enrichment equipment. Attached Figure Description

[0017] Figure 1 This is a partial exploded view of a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention.

[0018] Figure 2 This is a front view of a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention.

[0019] Figure 3 This is a rear view of a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention.

[0020] Figure 4 This is a cross-sectional view of the first substrate in a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention.

[0021] Icon labels:

[0022] 100-House; 200-Enrichment device; 210-Outlet; 220-Storage tank; 221-Storage cavity; 222-Inlet; 223-Outlet; 224-Bottle body; 225-Bottle cap; 226-Quick-connect male connector; 230-Transfer pipeline; 231-Air inlet; 232-Air outlet; 233-Waste liquid valve; 240-Filter assembly; 241-Ultrafiltration membrane; 242-Sealing gasket; 243-Support plate; 244-Through hole; 245-First U-groove; 250-Chuck; 251-Receiving cavity; 252-First cavity wall; 253-First opening; 256-First substrate; 257-Second substrate; 258-Groove; 259-Boss; 260- Fasteners; 261-First retaining ring; 262-Second retaining ring; 263-Fastening part; 264-Limiting hole; 270-Quick-connect female connector; 280-First aerosol filter; 300-Waste liquid collection device; 310-Liquid inlet; 320-Waste liquid main valve; 330-Second aerosol filter; 340-Collection bottle; 350-Vacuum pump; 360-Waste liquid collection pipeline; 370-Waste liquid transmission pipeline; 400-Pressure booster assembly; 410-Air pump; 430-Control switch; 440-Three-way valve; 450-Auxiliary pipeline; 451-High-pressure air interface; 452-High-pressure air outlet; 460-Check valve; 470-Pressure regulating valve; 480-Pressure gauge. Detailed Implementation

[0023] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0025] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0026] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0027] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0028] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0029] See Figure 1 , Figure 2 and Figure 3 , Figure 1 This is a partial exploded view of a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention. Figure 2 This is a front view of a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, provided in an embodiment of the present invention. Figure 3This is a rear view of a multi-channel rapid virus enrichment device for water based on ultrafiltration, according to an embodiment of the present invention. The multi-channel rapid virus enrichment device for water based on ultrafiltration, according to an embodiment of the present invention, includes a frame, multiple enrichment devices 200, and a waste liquid collection device 300. Each enrichment device 200 is connected to the frame, and the outlet 210 of each enrichment device 200 is connected to the inlet 310 of the waste liquid collection device 300. Each enrichment device 200 includes a storage tank 220, a delivery pipeline 230, and a filter assembly 240. The storage tank 220 has a storage cavity 221 for storing samples to be enriched. The cavity 221 has an inlet 222 and an outlet 223. The delivery pipeline 230 has an air inlet 231 and an air outlet 232. The delivery pipeline 230 is used to deliver high-pressure gas. The air outlet 232 is connected to the inlet 222 of the storage tank 220. The filter assembly 240 includes an ultrafiltration membrane 241, which corresponds to the outlet 223 of the storage tank 220, so that the sample to be enriched flowing out of the outlet 223 of the storage tank 220 is filtered through the ultrafiltration membrane 241 to form a contamination layer on the ultrafiltration membrane 241.

[0030] Specifically, this application increases the filtration area by setting multiple enrichment devices 200, thereby improving the enrichment efficiency of the sample to be enriched on the ultrafiltration membrane 241. In addition, the setting of the delivery pipeline 230 enables high-pressure gas to be delivered into the storage tank 220 through the inlet 222 of the delivery pipeline 230, that is, the sample to be enriched in the storage tank 220 is pressurized to pass through the ultrafiltration membrane 241 to achieve rapid enrichment.

[0031] In existing technologies, a common method is to use a gas pump 410 for suction, which provides power of less than 0.085 MPa through negative pressure filtration. Since the maximum negative pressure that the gas pump 410 can provide is a vacuum, the power provided by negative pressure filtration is limited. However, the ultrafiltration membrane 241 requires a transmembrane pressure greater than 0.15 MPa. This application uses a high-pressure gas method. Because the pressure of high-pressure gas is unrestricted, it can meet the transmembrane pressure required by the ultrafiltration membrane 241. Simultaneously, it allows for a larger filtration area and improved enrichment efficiency.

[0032] It should be noted that ultrafiltration is an enrichment method that uses an ultrafiltration membrane 241 with a molecular weight cutoff smaller than that of the virus to retain the virus in the water, and then recovers the virus by recycling the concentrate or the membrane fouling layer.

[0033] Furthermore, the enrichment devices 200 are arranged in a horizontal line to facilitate operation. Preferably, the number of enrichment devices 200 is 4-6.

[0034] See Figure 1In one embodiment, the filter assembly 240 further includes a chuck 250 and a sealing gasket 242. The chuck 250 has a receiving cavity 251. The first cavity wall 252 of the receiving cavity 251 has a first opening 253 communicating with the outlet 223. The sealing gasket 242 and the ultrafiltration membrane 241 are housed in the receiving cavity 251, and the sealing gasket 242 is located between the ultrafiltration membrane 241 and the first cavity wall 252 to seal the gap between the first opening 253 and the ultrafiltration membrane 241.

[0035] Specifically, one end of the sealing gasket 242 is tightly attached to the first chamber wall 252, and the other end is tightly attached to the ultrafiltration membrane 241, thereby sealing the gap between the first opening 253 and the ultrafiltration membrane 241. This ensures that the sample to be enriched flowing out through the first opening 253 is filtered by the ultrafiltration membrane 241, thus providing enrichment accuracy. Preferably, the sealing gasket 242 is a silicone gasket.

[0036] The receiving cavity 251 is a sealed cavity, and the ultrafiltration membrane 241 is housed in the receiving cavity 251 to ensure sealing, thus adapting to the high-pressure gas pressure mode.

[0037] Furthermore, in the height direction of the ultrafiltration membrane 241, the projected area of ​​the sealing gasket 242 is located outside the first opening 253, thereby avoiding obstruction of the first opening 253. Preferably, the sealing gasket 242 is annular and adapted to the shape of the first opening 253.

[0038] Furthermore, the chuck 250 is fixedly connected to the liquid storage tank 220 by a semi-permanent threaded fixation or a permanent welded fixation.

[0039] In one embodiment, the thickness of the sealing gasket 242 is 1mm-3mm, the inner diameter of the sealing gasket 242 is equivalent to the diameter of the first opening 253, and the outer diameter of the sealing gasket 242 is 5mm-10mm larger than the inner diameter.

[0040] In one embodiment, the ultrafiltration membrane 241 has a size of 100 mm or 150 mm, which is smaller than the outer diameter of the sealing gasket 242 and larger than the inner diameter of the sealing gasket 242. The ultrafiltration membrane 241 is made of polysulfone, polyethersulfone or polyvinylidene fluoride, and the molecular weight cut is preferably 100 kDa.

[0041] See Figure 1 In one embodiment, the second cavity wall opposite to the first cavity wall 252 of the receiving cavity 251 is provided with a second opening, which is configured as a liquid outlet 210. The filter assembly 240 also includes a support plate 243 disposed in the receiving cavity 251. The support plate 243 is provided with a through hole 244. The support plate 243 is located between the second cavity wall and the ultrafiltration membrane 241 and is used to support the ultrafiltration membrane 241.

[0042] Specifically, the second opening communicates with the first opening 253 through a through-hole 244, and the support plate 243 covers the second opening. One end of the support plate 243 abuts against the second cavity wall, and the other end abuts against the ultrafiltration membrane 241, thereby stably supporting the ultrafiltration membrane 241 and restricting its movement to ensure a stable seal of the sealing gasket 242. It should be noted that there are multiple through-holes 244, which are smaller than the second opening, thus ensuring stable support of the ultrafiltration membrane 241 by the support plate 243 and preventing dents in the area corresponding to the second opening of the ultrafiltration membrane 241 under impact.

[0043] In one embodiment, the support plate 243 is made of a smooth material such as 304 mirror stainless steel or polycarbonate, and its diameter is the same as the outer diameter of the sealing gasket 242.

[0044] See Figure 1 In one embodiment, the chuck 250 includes a first substrate 256 and a second substrate 257. One of the first substrate 256 and the second substrate 257 is provided with a groove 258, and the other is provided with a boss 259. The first substrate 256 and the second substrate 257 are engaged with the groove 258 through the boss 259 to form a receiving cavity 251.

[0045] Specifically, the first substrate 256 is provided with a boss 259 and the second substrate 257 is provided with a groove 258. The boss 259 and the groove 258 are connected and fitted together, which can play a sealing role. At the same time, it facilitates the installation and disassembly of the first substrate 256 and the second substrate 257, so as to facilitate the installation and disassembly of the ultrafiltration membrane 241.

[0046] In one embodiment, the chuck 250 is made of 304 stainless steel or polycarbonate material and has a pressure resistance greater than 0.25 MPa.

[0047] See Figure 1 and Figure 4 , Figure 4 This is a cross-sectional view of the first substrate in a multi-channel rapid enrichment device for viruses in water based on ultrafiltration, according to an embodiment of the present invention. In one embodiment, the first substrate 256 is provided with a first U-shaped groove 245, and the groove wall of the first U-shaped groove 245 is configured as a first cavity wall 252.

[0048] Specifically, the first substrate 256 is located above the second substrate 257, the first U-shaped groove 245 is inverted U-shaped, and the first opening 253 is located at the top of the first U-shaped groove 245, so that the sample to be enriched flowing out through the first outlet 223 slides naturally along the first cavity wall 252, thereby improving the uniformity of water distribution.

[0049] Furthermore, a second U-shaped groove is provided on the second substrate 257, the groove wall of the second U-shaped groove is configured as a second cavity wall, and the second opening is located at the lower end of the second U-shaped groove to facilitate the natural collection of filtered waste liquid.

[0050] See Figure 1 In one embodiment, the filter assembly 240 further includes a fastener 260, which includes a first retaining ring 261, a second retaining ring 262, and a fastening portion 263. A limiting hole 264 for the chuck 250 to pass through is defined between the first retaining ring 261 and the second retaining ring 262. The first retaining ring 261 and the second retaining ring 262 are connected by the fastening portion 263 to abut against the first substrate 256 and the second substrate 257 to limit the first substrate 256 and the second substrate 257 from moving away from each other.

[0051] Specifically, the fastening part 263 securely connects the first retaining ring 261 and the second retaining ring 262, thereby stably clamping the first substrate 256 and the second substrate 257. This increases the friction between the first substrate 256 and the second substrate 257, preventing them from moving away from each other and improving the sealing of the cavity 251 within the chuck 250. Preferably, the fastening part 263 is a screw.

[0052] See Figure 1 , Figure 2 and Figure 3 In one embodiment, the liquid storage tank 220 includes a bottle body 224 and a bottle cap 225. The bottle cap 225 is threadedly connected to the bottle body 224. A storage cavity 221 is disposed on the bottle body 224. An outlet 223 is disposed on the bottle body 224. An inlet 222 is disposed on the bottle cap 225. A first aerosol filter 280 covering the inlet 222 is installed on the bottle cap 225.

[0053] Specifically, after filtration, disconnecting the delivery line 230 from the inlet 222 allows for pressure relief. The cap 225 is equipped with a first aerosol filter 280 covering the inlet 222, ensuring that all gas exiting through the inlet 222 passes through this filter, preventing virus spillage and protecting personnel and the environment. The threaded connection between the cap 225 and the bottle body 224 facilitates easy installation and removal, allowing for convenient placement of the sample to be enriched while maintaining a tight seal.

[0054] See Figure 1 and Figure 2In one embodiment, the bottle cap 225 is provided with a quick-connect male connector 226, and the delivery pipe 230 is provided with a quick-connect female connector 270. The quick-connect female connector 270 and the quick-connect male connector 226 are engaged, thereby facilitating the disconnection of the delivery pipe 230 from the inlet 222 for convenient pressure release. Preferably, the quick-connect male connector 226 is a self-locking male connector, and the quick-connect female connector 270 is a self-locking female connector, thereby ensuring airtightness under high pressure.

[0055] See Figure 1 In one embodiment, the enrichment device 200 further includes a pressurization component 400, which includes an air pump 410. The air inlet 231 of the delivery pipeline 230 is connected to the output port of the corresponding air pump 410. A pressure regulating valve 470 is provided on the delivery pipeline 230 to regulate the pressure of the gas output by the corresponding air pump 410.

[0056] Specifically, the pressure of the gas output from the air pump 410 is adjusted by the pressure regulating valve 470, thereby regulating the transmembrane velocity of the sample to be enriched, improving the enrichment efficiency, and adapting to different filter area sizes, thus enhancing the adaptability of the enrichment equipment. Preferably, the air pump 410 is a brushless diaphragm air pump with a minimum pressure greater than 0.3 MPa.

[0057] Furthermore, a pressure sensor is installed on the delivery pipeline 230 to detect the pressure of the gas being delivered by the corresponding delivery pipeline 230. The pressure sensor is electrically connected to a pressure gauge 480 to display the pressure of the gas being delivered by the corresponding delivery pipeline 230.

[0058] Furthermore, the multi-channel rapid virus enrichment device in water based on ultrafiltration also includes an auxiliary pipeline 450. The auxiliary pipeline 450 includes a high-pressure gas interface 451 and a high-pressure gas outlet 452. The high-pressure gas interface 451 is used to connect to an external gas source, and the high-pressure gas outlet 452 is connected to the air inlet 231 of each delivery pipeline 230, thus enabling power switching under existing high-pressure gas source conditions and improving reliability. A one-way valve 460 is installed on the auxiliary pipeline 450 to control the flow direction.

[0059] The water-based multi-channel rapid enrichment device for viruses based on ultrafiltration also includes a three-way valve 440. The two valve ports of the three-way valve 440 are connected to the high-pressure gas outlet 452 and the output port of the air pump 410, respectively, and the other valve port is connected to the air inlet 231 of each delivery pipeline 230.

[0060] See Figure 1 , Figure 2 , Figure 3In one embodiment, the waste liquid collection device 300 includes a collection bottle 340 and a vacuum pump 350. The inlet 310 is provided on the collection bottle 340. The collection bottle 340 is also provided with an air intake that communicates with the output port of the vacuum pump 350. A second anti-aerosolization filter 330 covering the air intake is installed between the collection bottle 340 and the vacuum pump 350.

[0061] Specifically, the vacuum pump 350 provides negative pressure to facilitate the rapid collection of filtered waste liquid during the enrichment process. A second aerosol filter 330, covering the suction port, is installed between the collection bottle 340 and the vacuum pump 350. This ensures that all gas discharged through the suction port passes through the second aerosol filter 330, preventing virus spillage and protecting the safety of operators and the environment.

[0062] See Figure 1 In one embodiment, each enrichment device 200 further includes a waste liquid transmission pipeline 370 connected to the outlet 210 and the inlet 310, and each waste liquid transmission pipeline 370 is also equipped with a waste liquid valve 233. The waste liquid collection device 300 further includes a waste liquid collection pipeline 360, the outlet 210 is connected to the inlet 310 through the waste liquid collection pipeline 360, and the waste liquid collection pipeline 360 ​​is equipped with a waste liquid main valve 320.

[0063] See Figure 1 , Figure 2 and Figure 3 In one embodiment, the multi-channel rapid enrichment device for viruses in water based on ultrafiltration also includes a housing 100, which is constructed as a frame. Various waste liquid transmission pipelines 370 converge within the housing 100 and are introduced into a collection bottle 340 via a waste liquid collection pipeline 360. An air pump 410 is disposed within the housing 100, and pressure regulating valves 470, pressure gauges 480, and waste liquid valves 233 are mounted on the outer wall of the housing 100. A control switch 430 for independently controlling the air pump 410 is also provided on the outer wall of the housing 100.

[0064] In one embodiment, the housing 100 is made of 304 mirror stainless steel, aluminum alloy or high-density polyethylene, preferably 304 mirror stainless steel.

[0065] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0066] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A multi-channel rapid enrichment device for viruses in water based on ultrafiltration, characterized in that, The ultrafiltration-based multi-channel rapid virus enrichment device in water includes a frame, multiple enrichment devices, and a waste liquid collection device. Each enrichment device is connected to the frame, and the outlet of each enrichment device is connected to the inlet of the waste liquid collection device. Each enrichment device includes a storage tank, a delivery pipeline, and a filtration assembly. The storage tank has a storage cavity for storing samples to be enriched, and the storage cavity has an inlet and an outlet. The delivery pipeline has an air inlet and an air outlet for delivering high-pressure gas. The air outlet is connected to the inlet of the storage tank. The filtration assembly includes an ultrafiltration membrane, which corresponds to the outlet of the storage tank, so that the samples to be enriched flowing out of the outlet of the storage tank are filtered through the ultrafiltration membrane to form a contamination layer on the ultrafiltration membrane. The filtration assembly further includes a chuck and a sealing gasket. The chuck has a receiving cavity. The first cavity wall of the receiving cavity has a first opening communicating with the outlet. The sealing gasket and the ultrafiltration membrane are housed in the receiving cavity, and the sealing gasket is located between the ultrafiltration membrane and the first cavity wall to seal the gap between the first opening and the ultrafiltration membrane. The receiving cavity has a second opening on the second cavity wall opposite to the first cavity wall. The second opening is configured as the liquid outlet. The filtration assembly also includes a support plate housed in the receiving cavity. The support plate has through holes and is located between the second cavity wall and the ultrafiltration membrane. The support plate is used to support the ultrafiltration membrane. The chuck includes a first substrate and a second substrate. One of the first substrate and the second substrate is provided with a groove, and the other is provided with a boss. The first substrate and the second substrate are inserted and engaged with the groove through the boss to form the receiving cavity. The filter assembly further includes a fastener, which includes a first retaining ring, a second retaining ring, and a fastening part. The first retaining ring and the second retaining ring define a limiting hole for the chuck to pass through. The first retaining ring and the second retaining ring are connected by the fastening part to abut against the first substrate and the second substrate, thereby limiting the first substrate and the second substrate from moving away from each other.

2. The multi-channel rapid enrichment device for viruses in water based on ultrafiltration according to claim 1, characterized in that, The first substrate is provided with a first U-shaped groove, and the groove wall of the first U-shaped groove is constructed as the first cavity wall.

3. The multi-channel rapid enrichment device for viruses in water based on ultrafiltration according to any one of claims 1-2, characterized in that, The liquid storage tank includes a bottle body and a bottle cap. The bottle cap is threadedly connected to the bottle body. The storage cavity is disposed in the bottle body. The outlet is disposed on the bottle body. The inlet is disposed on the bottle cap. A first aerosol filter covering the inlet is installed on the bottle cap.

4. The multi-channel rapid enrichment device for viruses in water based on ultrafiltration according to claim 3, characterized in that, The bottle cap is provided with a quick-connect male connector, and the delivery pipeline is provided with a quick-connect female connector, which engages with the quick-connect male connector.

5. The multi-channel rapid enrichment device for viruses in water based on ultrafiltration according to any one of claims 1-2, characterized in that, The enrichment device further includes a pressurization component, which includes an air pump, and the air inlet of the delivery pipeline is connected to the corresponding output port of the air pump. The delivery pipeline is equipped with a pressure regulating valve for adjusting the pressure of the gas output from the corresponding air pump; and / or, the delivery pipeline is equipped with a pressure sensor for detecting the pressure of the gas delivered in the corresponding delivery pipeline.

6. The multi-channel rapid enrichment device for viruses in water based on ultrafiltration according to any one of claims 1-2, characterized in that, The waste liquid collection device includes a collection bottle and a vacuum pump. The liquid inlet is located on the collection bottle. The collection bottle is also provided with an air intake that communicates with the output port of the vacuum pump. A second anti-aerosolization filter is installed between the collection bottle and the vacuum pump, covering the air intake.

Images