A triple fluorescence quantitative PCR detection kit for tick-borne rickettsia

Abstract

The invention discloses a triple fluorescence quantitative PCR detection kit for tick-borne rickettsia, and belongs to the field of biotechnology detection. The triple fluorescence quantitative PCR detection kit for tick-borne rickettsia includes a primer combination consisting of a sequence shown in SEQ ID NO.1‑6. The triple fluorescence quantitative PCR detection kit for tick-borne rickettsia provided by the invention can simultaneously detect tick-borne rickettsia laurensis, rickettsia sibirica and anaplasma, realizes the typing detection of spotted fever group rickettsia species, overcomes the difficulty of rapid differential diagnosis of the above three tick-borne rickettsia at the same time, and has the advantages of simple operation, strong specificity and economical and practical.

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