Use of rhizoma anemarrhenae in preparation of related products for poultry

Abstract

The application provides use of Chuan Sheng Gan or its extract in preparation of a product for promoting growth of chickens or / and preventing or treating newcastle disease, and expands the use range of Chuan Sheng Gan.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine, and further to new uses of traditional Chinese medicine in poultry farming. Background Technology

[0002] Iridis Tectori Rhizoma is the dried rhizome of the iris plant (Iridaceae family). It is bitter and cold in nature, and enters the lung meridian. It is a species listed in the Chinese Pharmacopoeia and is a traditional Chinese medicine from Sichuan province. Studies have shown that Iridis Tectori extract contains isoflavones and their glycosides, flavonoids and their glycosides, benzoquinones, triterpenoids and their saponins, and other active ingredients. Current research indicates that Iridis Tectori possesses various functions including antipyretic, antitussive, analgesic, anti-inflammatory, antibacterial, antiviral, antioxidant, and antitumor effects.

[0003] However, there are few reports on research on the use of Belamcanda chinensis in poultry farming. Summary of the Invention

[0004] This invention aims to explore the potential role of Belamcanda chinensis in poultry farming and strive to expand its application scope.

[0005] Based on the experimental content of this invention, this invention provides the use of Belamcanda chinensis or its extracts in the preparation of products that promote chicken growth and / or prevent or treat Newcastle disease.

[0006] Furthermore, promoting chicken growth refers to increasing feed intake and increasing body weight.

[0007] The improvement in feed intake mentioned in this invention refers to the improvement in feed intake of chickens after using Belamcanda chinensis extract, compared to using only basic feed without using Belamcanda chinensis extract.

[0008] The increase in weight mentioned in this invention refers to the improvement in weight gain in chickens after using Belamcanda chinensis extract, compared to using only basic feed without using Belamcanda chinensis extract.

[0009] The increased feed intake and weight gain described in this invention are achieved under conditions of low feed conversion ratio.

[0010] The formula for calculating feed conversion ratio (F / G) is as follows: Feed conversion ratio (F / G) = Feed consumption / Weight gain

[0011] According to the formula for feed conversion ratio, the lower the feed conversion ratio, the higher the feed conversion rate.

[0012] Newcastle disease (ND) is an acute, febrile, septicemic, and highly contagious infectious disease of poultry caused by the Newcastle disease virus. It is characterized by high fever, respiratory distress, diarrhea, neurological disorders, and hemorrhage of the mucous membranes and serous membranes. With a high morbidity and mortality rate, it is a major infectious disease threatening the poultry industry.

[0013] Furthermore, the prevention or treatment of Newcastle disease refers to increasing or maintaining Newcastle disease antibody levels.

[0014] Antibodies are protective proteins produced by the body in response to stimulation by antigens.

[0015] The extract of *Belamcanda chinensis* is selected from the aqueous extract and / or alcoholic extract of *Belamcanda chinensis*.

[0016] The alcohol extract mentioned in this invention refers to an ethanol extract.

[0017] In this invention, the ethanol is selected from 50-95% ethanol.

[0018] In this invention, each 1g of the extract of Belamcanda chinensis contains not less than 50.0mg of Belamcanda chinensis glycoside.

[0019] Furthermore, the extraction method for the *Belamcanda chinensis* extract is as follows:

[0020] (1) Belamcanda chinensis, water extraction, concentration of water extract, addition of equal amount of 95% ethanol, alcohol precipitation, supernatant for use.

[0021] (2) The alcohol precipitation precipitate was dissolved in 50% ethanol and the supernatant was kept for later use;

[0022] (3) Combine the supernatants from the first two steps, recover the ethanol, concentrate and dry to obtain the final product.

[0023] The dosage of Belamcanda chinensis extract is 0.25% to 1.0% of the basal diet.

[0024] Furthermore, the product is a feed containing 0.25% to 1.0% Belamcanda chinensis extract.

[0025] The present invention also provides a chicken feed containing 0.25% to 1.0% Belamcanda chinensis extract.

[0026] This invention has found that when the amount of Belamcanda chinensis extract used is 0.25% of the basal diet, it can significantly increase the feed intake of chicks, increase daily weight gain, and improve the production performance of chicks.

[0027] In this invention, the extract of Belamcanda chinensis can exist as the sole active ingredient, or it can be used in combination with other products with similar activities.

[0028] When it is the sole active ingredient, the feed does not contain any other Chinese herbal extracts. Detailed Implementation

[0029] The extract of Belamcanda chinensis contains at least 50.0 mg of Belamcanda chinensis glycoside per 1g.

[0030] The Belamcanda chinensis extract used in this invention can be obtained by purchasing commercially available products or prepared using existing technologies, for example, by the following methods:

[0031] Slice the medicinal material, boil it twice with 8 times the amount of water, 1.5 hours each time, filter, concentrate the filtrate to the amount of medicinal material, add an equal amount of 95% ethanol, stir well, let stand for 12 hours, separate the supernatant, filter, recover the ethanol until there is no alcohol odor, dissolve the alcohol precipitate with 2 times the amount of 50% ethanol, stir well, let stand for 12 hours, take the supernatant, filter, recover the ethanol until there is no alcohol odor, combine the recovered liquids, add an equal amount of water, mix well, centrifuge at high speed, concentrate the centrifuged liquid under reduced pressure, dry, pulverize and mix to obtain the final product.

[0032] Example 1

[0033] 1. Materials and Methods

[0034] 1.1 Experimental animals and grouping

[0035] Two hundred healthy one-day-old Daheng 799 hens were selected and randomly divided into four groups, with five replicates per group and ten hens per replicate. They were then housed in separate cages. Feeding and management were carried out in accordance with the "Daheng 799 Feeding and Management Manual".

[0036] 1.2 Test Materials

[0037] ITR (Iris tectorum extract): Prepared by Sanxingdui Biotechnology Co., Ltd., Guanghan City, Sichuan Province. 1g of extract is equivalent to 10g of raw herb. ITR contains 61.3mg / g of irisin.

[0038] Newcastle disease live vaccine (La Sota strain): produced by Guangxi Liyuan Biotechnology Co., Ltd., batch number 120325.

[0039] 1.3 Feeding and Management

[0040] Clean and fumigate the chicken coop and equipment, and leave it empty for two weeks before raising chickens. Provide 24-hour lighting for the first two days, 23-hour lighting for days 3-7, and gradually transition to natural light in the second week; maintain the temperature at 35-36℃, and then decrease the temperature by 1-2℃ each week until it reaches room temperature; maintain the humidity at 65%-75%.

[0041] The experimental period was 42 days. At 7 days old, chickens were vaccinated with Newcastle disease live vaccine (La Sota strain) via nasal drops. From 1 day old to the end of the experiment at 42 days old, Group I (the blank control group) was fed a basal diet without antibiotics or any added drugs; Groups II, III, and IV (the drug groups) had 0.25%, 0.5%, and 1.0% ITR added to their basal diet, respectively. The basal diet was formulated according to the nutritional requirements of broiler chicks in the "Chicken Feeding Standard" (NY / T33-2004), using a corn-soybean meal type feed in powder form. The entire experimental period was divided into two feeding stages: broiler feed for 1-21 days old and broiler feed for 22-42 days old. The composition and nutritional levels of the basal diet for each stage are shown in Table 1.

[0042] Table 1. Composition and Nutritional Levels of Basal Diets (Air-Dried Basis)

[0043]

[0044]

[0045] 1.4 Monitoring Indicators

[0046] 1.4.1 Growth performance

[0047] Chicks in each group were weighed on an empty stomach at 1, 14, 28, and 42 days of age, and their feed intake was recorded. The average daily gain (ADG) and average daily feed intake (ADFI) of each group were calculated.

[0048] Feed conversion ratio (F / G) = Feed consumption / Weight gain.

[0049] 1.4.2 Newcastle disease antibody testing

[0050] Newcastle disease antibody detection was performed using the ELLISA kit for Newcastle disease antibody detection in chickens provided by Qingdao Luyuan Biotechnology Co., Ltd.

[0051] 1.4.3 Data Statistical Analysis

[0052] Data were analyzed using SPSS 24.0 statistical software. One-way ANOVA was used for one-way analysis of variance, and Duncan's method was used for multiple comparisons. Results are expressed as mean ± standard deviation (SD), and P < 0.05 was considered statistically significant.

[0053] 2 Results and Analysis

[0054] 2.1 Effects of "Belamcanda chinensis extract" on the growth performance of chicks

[0055] As shown in Table 2, compared with Group I (blank control), Group II (0.25% ITR) significantly increased the body weight of the experimental chickens at 42 days of age from 1 to 42 days. There were no significant differences in the average daily feed intake, average daily weight gain and feed conversion ratio at each stage of the experimental chickens (P>0.05). However, with the increase of drug concentration, the average daily feed intake and average daily weight gain showed a decreasing trend, while the feed conversion ratio showed an increasing trend.

[0056] Table 2 Effects of ITR on the growth performance of parent breeder chickens of Daheng 799

[0057]

[0058] Note: Different letters in the superscript of the same data indicate significant differences (P<0.05), while no letter or the same letter in the superscript indicates no significant differences (P>0.05).

[0059] 2.2 Effect of "Belamcanda chinensis extract" on Newcastle disease antibody levels

[0060] Table 3 shows that there were no significant differences in Newcastle disease antibody levels at different stages of the experiment. However, the Newcastle disease antibody levels showed a decreasing trend with increasing age. The ITR dosage groups showed some resistance to the decrease in Newcastle disease antibody levels. At 42 days of age, the antibody levels in all drug groups were higher than those in the blank control group. This indicates that ITR can effectively protect the maintenance of Newcastle disease antibody levels in chicken flocks.

[0061] Table 3. Effects of ITR on Newcastle disease antibody levels (A450, OD value)

[0062]

[0063]

[0064] Note: Different letters in the superscript of the same data indicate significant differences (P<0.05), while no letter or the same letter in the superscript indicates no significant differences (P>0.05).

[0065] 3 Discussion

[0066] 3.1 Effects of "Belamcanda chinensis extract" on the growth performance of chicks

[0067] During the experiment, compared with Group I (blank control), Group II (0.25% ITR) significantly increased the body weight of the chickens at 42 days of age throughout the entire experimental period. There were no significant differences in average daily feed intake, average daily weight gain, and feed conversion ratio among the other drug groups (P>0.05). Furthermore, Group II (0.25% ITR) had the highest average daily feed intake and average daily weight gain, and the lowest feed conversion ratio. This indicates that Group II (0.25% ITR) had a better feed conversion ratio, suggesting that the growth-promoting effect of ITR is dose-dependent; 0.25% ITR can significantly increase feed intake, increase daily weight gain, and improve the growth performance of chicks.

[0068] 3.2 Effects of "Radix Belamcandae Persicae Extract" on Newcastle Disease Antibodies

[0069] The experiment used a single intranasal immunization at 7 days of age. After 14 days of age, the Newcastle disease antibody level in chickens showed a clear downward trend with increasing age. This was mainly because a live Newcastle disease vaccine was used at 7 days of age, which provides protection immediately after vaccination, with antibodies reaching their peak one week later and then declining. Although there were no significant differences between the ITR dosage groups and the control group, the antibody OD values ​​detected in the 0.25% dosage group were higher than those in the control group at all age stages, indicating that adding 0.25% ITR can delay the decline in Newcastle disease antibody levels and effectively protect the flock by maintaining Newcastle disease antibody levels.

Claims

1. The use of Belamcanda chinensis extract as the sole active ingredient in the preparation of products that promote the growth of chicks; the amount of Belamcanda chinensis extract used is 0.25% of the basal diet; each 1g of the Belamcanda chinensis extract contains not less than 50.0mg of Belamcanda chinensis glycoside; in, The promotion of chick growth refers to increasing feed intake, increasing body weight, and reducing feed conversion ratio; The extraction method of the *Belamcanda chinensis* extract is as follows: (1) Belamcanda chinensis, water extraction, concentration of water extract, addition of equal volume of 95% ethanol, alcohol precipitation, supernatant for use; (2) The precipitate was dissolved in 50% ethanol and the supernatant was kept for later use; (3) Combine the supernatants from the first two steps, recover the ethanol, concentrate and dry to obtain the final product.

2. The use according to claim 1, characterized in that, The product is a feed containing 0.25% Belamcanda chinensis extract.

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