Immunoassay method

Abstract

The application relates to an immunoassay method comprising the following steps: moving a magnetic collection assembly carrying a vacant reactor to a pipetting station, sucking samples and reagents in the carrying assembly through a sampling element and injecting into the reactor, and moving the reactor out of the magnetic collection assembly through a gripper to mix the liquid in the reactor; incubating the mixed reactor; moving the incubated reactor into the magnetic collection assembly through the gripper and moving to the pipetting station, collecting magnetic beads in the reactor on the inner wall of the reactor, and sucking waste liquid through the sampling element; moving the magnetic collection assembly carrying the reactor after sucking the waste liquid to a liquid injection station, injecting cleaning liquid into the reactor, and moving the reactor out of the magnetic collection assembly through the gripper to mix the liquid in the reactor; moving the reactor containing the cleaning liquid into the magnetic collection assembly through the gripper and moving to the pipetting station, sucking waste liquid through the sampling element to measure, so that the manufacturing cost and volume of the immunoassay instrument can be reduced.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to an immunoassay method. Background Technology

[0002] Immunoassay analyzers have gained increasingly widespread application in recent years due to their advantages such as high sensitivity, good specificity, wide linear range, high throughput, strong platform scalability, and rich menu of measurable items. However, for traditional immunoassay methods, although the throughput is high, the immunoassay analyzers used to perform these methods are expensive, bulky, and require highly specialized operation, making them unsuitable for use in small and medium-sized laboratories or medical institutions, thus limiting the availability of immunoassay methods. Summary of the Invention

[0003] One technical problem addressed by this application is how to reduce the manufacturing cost and size of immunoassay analyzers that perform immunoassay methods.

[0004] An immunoassay method involves a sampling element and a gripper moving linearly along three directions (a first direction, a second direction, and a third direction) arranged at angles to each other, while a magnetic collection assembly carrying a reactor moves linearly between pipetting and injection stations spaced apart along the second direction. The immunoassay method includes the following steps:

[0005] An empty reactor is transferred to the magnetic collection assembly by a gripper. The magnetic collection assembly carries the empty reactor to the pipetting station. Samples and reagents are added to the reactor through the sampling device. The reactor is then removed from the magnetic collection assembly by the gripper to mix the liquid in the reactor.

[0006] The mixed reactor was incubated.

[0007] The gripper moves the incubated reactor into the magnetic collection assembly and then to the liquid transfer station, where magnetic beads are collected on the inner wall of the reactor, and waste liquid is extracted through the sampling device.

[0008] The reactor, after being sucked up by the magnetic collection component, is moved to the liquid injection station. Cleaning liquid is injected into the reactor through the first liquid injection component, and the reactor is moved out of the magnetic collection component by the gripper to mix the liquid in the reactor.

[0009] The reactor containing the cleaning solution is moved into the magnetic collection assembly by the gripper and then moved to the liquid transfer station, where the waste liquid is drawn up by the sampling element.

[0010] The magnetic collection assembly carries the reactor to the injection station, and the substrate liquid is injected into the reactor through the second injection assembly; and

[0011] The reactor containing the substrate liquid is transferred to the measuring mechanism for measurement using the gripper.

[0012] In one embodiment, the liquid in the reactor is mixed using the same mixing mechanism.

[0013] In one embodiment, the sampling device is cleaned after it has been injected with a sample, reagent, or waste liquid.

[0014] In one embodiment, the step of removing waste liquid from the reactor after the reaction liquid has been completely absorbed, followed by the injection of cleaning liquid, is denoted as a first-stage cleaning and separation process. The immunoassay method includes multiple stages of such cleaning and separation processes.

[0015] In one embodiment, the number of injection stations is two, referred to as the first injection station and the second injection station, respectively. Cleaning liquid is injected into the reactor at the first injection station; after the reactor has absorbed the waste liquid for the last time, substrate liquid is injected into the reactor at the second injection station.

[0016] In one embodiment, the reactor containing the reaction liquid is moved to the injection station to inject cleaning liquid before the waste liquid is drawn from the reactor.

[0017] In one embodiment, between injecting the substrate liquid into the reactor and taking measurements, the reactor is moved out of the magnetic collection assembly by a gripper for incubation.

[0018] In one embodiment, the reactor is supported by a fixed incubation mechanism for incubation.

[0019] In one embodiment, the magnetic collection assembly has a sample loading position and a collection position for carrying the reactor, the sample loading position and the collection position being spaced apart along the movement direction of the magnetic collection assembly, the sample loading position being movable to the pipetting station, and the collection position being movable to the pipetting station or the injection station.

[0020] In one embodiment, there are multiple collection locations. For two adjacent collection locations, the reactor injects cleaning liquid into one collection location and then moves to the other collection location to collect waste liquid.

[0021] One technical advantage of one embodiment of this application is that, given that the sampling component can perform three-dimensional linear motion, it has three degrees of freedom. Furthermore, the carrier assembly holding the reaction liquid is fixedly positioned, allowing the sampling component to draw the reaction liquid from the carrier assembly through three-dimensional linear motion. The transfer component can also perform three-dimensional linear motion, thus having three degrees of freedom, ensuring smooth transfer of the reactor to a designated position. Simultaneously, the magnetic collection assembly carrying the reactor moves linearly between the pipetting station and the injection station spaced apart along the second direction. The resulting immunoassay method simplifies the structure of the immunoassay analyzer, thereby reducing the manufacturing cost and size of the analyzer. Attached Figure Description

[0022] Figure 1 This is a three-dimensional structural schematic diagram of an immunoassay analyzer provided in one embodiment.

[0023] Figure 2 for Figure 1 The diagram shows a partial three-dimensional structure of the immunoassay analyzer chassis after some structures have been removed.

[0024] Figure 3 for Figure 1 The diagram shows a partial three-dimensional structure of the immunoassay analyzer after the entire chassis has been removed.

[0025] Figure 4 for Figure 3 A top-view structural diagram.

[0026] Figure 5 for Figure 1 The diagram shows a partial three-dimensional structure of the immunoassay analyzer, including the input mechanism and the incubation mechanism.

[0027] Figure 6 for Figure 1 The diagram shows another partial three-dimensional structure of the immunoassay analyzer after some structures have been removed.

[0028] Figure 7 for Figure 6 A schematic diagram of the three-dimensional structure from another perspective.

[0029] Figure 8 for Figure 1 The diagram shows a partial three-dimensional structure of the immunoassay analyzer after some structures have been removed.

[0030] Figure 9 for Figure 8 A partial three-dimensional structural diagram after removing the consumables mechanism.

[0031] Figure 10 for Figure 1 The diagram shows a three-dimensional structural schematic of the magnetic collection component in the immunoassay analyzer.

[0032] Figure 11 This is a flowchart illustrating the process flow of an immunoassay method provided in one embodiment.

[0033] Reference numerals: Immunoassay analyzer 10, reactor 20, input mechanism 100, input component 110, card slot 111, import component 120, input slide 121, consumable mechanism 200, sample tube 210, reagent kit 220, sampling mechanism 300, sampling component 310, first sampling drive module 321, second sampling drive module 322, third sampling drive module 323, transport mechanism 400, gripper 410, first transport drive module 421, second transport drive module 422, third transport drive module 423, incubation mechanism 500, magnetic collection mechanism 600, pipetting station 61 0. Liquid injection station 620, first liquid injection station 621, second liquid injection station 622, transfer station 630, magnetic collection assembly 640, carrier 641, groove 6411, magnetic component 642, collection position 643, first collection position 6431, second collection position 6432, third collection position 6433, fourth collection position 6434, sample addition position 644, belt drive assembly 650, liquid injection mechanism 700, first liquid injection assembly 710, second liquid injection assembly 720, mixing mechanism 810, output mechanism 820, output slide 821, measuring mechanism 830, chassis 840. Detailed Implementation

[0034] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0035] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0036] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0037] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0038] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0039] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0040] See Figure 1 , Figure 2 , Figure 3 and Figure 4An immunoassay analyzer 10 provided in one embodiment of this application includes an input mechanism 100, a consumable mechanism 200, a sampling mechanism 300, a transport mechanism 400, an incubation mechanism 500, a magnetic collection mechanism 600, a liquid injection mechanism 700, a mixing mechanism 810, an output mechanism 820, a measuring mechanism 830, and a chassis 840. The chassis 840 can serve as a housing, allowing the input mechanism 100, consumable mechanism 200, sampling mechanism 300, incubation mechanism 500, transport mechanism 400, magnetic collection mechanism 600, liquid injection mechanism 700, mixing mechanism 810, output mechanism 820, and measuring mechanism 830 to all be housed within the chassis 840.

[0041] See Figure 5 In some embodiments, the input mechanism 100 is used to transport and carry an empty and clean reactor 20. The input mechanism 100 includes an input component 110 and an inlet component 120. The input component 110 may be generally disc-shaped, and its edge has a slot 111, which is recessed to a certain depth radially along the input component 110. Multiple slots 111 are spaced apart circumferentially along the input component 110, and the reactor 20 can be inserted into the slot 111, thus enabling the input component 110 to carry the reactor 20. The inlet component 120 has an input slide 121, the extension direction of which forms an acute angle with the direction of gravity of the reactor 20 located within the input slide 121. The end of the input slide 121 can correspond to and communicate with one of the slots 111. During the rotation of the input component 110, the end of the input slide 121 can alternately communicate with different slots 111.

[0042] During the operation of the input mechanism 100, empty and clean reactors 20 can be placed into the input chute 121 by means of a robotic arm or manually. The input chute 121 can buffer and sort the reactors 20. When the input component 110 rotates and one of the slots 111 connects with the end of the input chute 121, a reactor 20 in the input chute 121 will automatically fall into the slot 111 under the action of gravity. When the input component 110 rotates at a certain angle and the next adjacent slot 111 connects with the end of the input chute 121, another reactor 20 in the input chute 121 will automatically fall into the slot 111. Therefore, through the intermittent rotation of the input component 110, different slots 111 can be alternately connected with the end of the input chute 121, so that the reactors 20 in the input chute 121 fall into different slots 111 in sequence. In this way, the input mechanism 100 can realize the conveying and buffering function of the reactors 20.

[0043] If the reactors 20 are first randomly placed into the hopper of the input mechanism 100, and then sorted by a sorting component for input, the entire input mechanism 100 will be structurally complex, have relatively high manufacturing costs, be bulky, and be prone to reactor jamming. If disposable trays are used to pre-sort the reactors 20, this will also result in larger trays and increased usage costs.

[0044] As for the input mechanism 100 in the above embodiments, the cooperation between the input component 110 and the inlet component 120 can realize the conveying and buffering of the reactor 20. This can simplify the structure of the input mechanism 100 and reduce manufacturing costs, make the input mechanism 100 more compact in structure and reduce its volume, and also eliminate the jamming phenomenon of the reactor 20, reduce the failure rate of the input mechanism 100 and improve operational reliability.

[0045] See Figure 2 , Figure 3 and Figure 4 In some embodiments, the consumable mechanism 200 includes a support component with a sample rack loading slot and a reagent kit 220 loading slot. The support component is fixed relative to the chassis 840, meaning it cannot move relative to the chassis 840. The sample rack can be placed into or removed from the sample rack loading slot, and the reagent kit 220 can be placed into or removed from the reagent kit 220 loading slot. Multiple sample tubes 210 are inserted into the sample rack, and each sample tube 210 can hold a sample to be tested. The reagent kit 220 contains reagents. In some embodiments, a sample rack support is slidably mounted on the support component, and the sample rack is placed on the sample rack support. The sample rack support can be pushed into or pulled out of the sample rack slot. A reagent kit 220 support is slidably mounted on the support component, and the reagent kit 220 is placed on the reagent kit 220 support. The reagent kit 220 support can be pushed into or pulled out of the reagent kit 220 slot. Because the sample rack support and the reagent kit 220 support can be pushed into and pulled out, the loading and unloading of the sample rack and the reagent kit 220 is convenient.

[0046] The first direction, the second direction, and the third direction are three different directions. The first direction, the second direction, and the third direction can be set at an angle to each other, for example, the first direction, the second direction, and the third direction can be perpendicular to each other. In this way, the first direction, the second direction, and the third direction can be the extension directions of the three coordinate axes in the spatial rectangular coordinate system. For example, the first direction can be the X-axis direction, the second direction can be the opposite direction of the Y-axis, and the third direction can be the Z-axis direction. The direction of gravity of the reactor 20 in the input slide 121 is the third direction.

[0047] See Figure 2 , Figure 3, Figure 4 and Figure 6 In some embodiments, the sampling mechanism 300 may include a sampling element 310, a first sampling driving module 321, a second sampling driving module 322, and a third sampling driving module 323. The first sampling driving module 321 may extend along a first direction, the second sampling driving module 322 may extend along a second direction, and the third sampling driving module 323 may extend along a third direction. The second sampling driving module 322 may be slidably connected to the first sampling driving module, and the first sampling driving module 321 is used to drive the second sampling driving module 322 to reciprocate along the first direction. The third sampling driving module 323 may be slidably connected to the second sampling driving module 322, and the second sampling driving module 322 is used to drive the third sampling driving module 323 to reciprocate along the second direction. The sampling element 310 is slidably disposed on the third sampling driving module 323, and the third sampling driving module 323 is used to drive the sampling element 310 to reciprocate along the third direction. The sampling element 310 may be a needle-like structure, etc. Therefore, when the first sampling drive module 321, the second sampling drive module 322 and the third sampling drive module 323 move, the sampling component 310 can move linearly relative to the chassis 840 along the first direction, the second direction and the third direction, so that the sampling component 310 has three degrees of freedom of linear motion.

[0048] By performing three-degree-of-freedom linear motion, the sampling element 310 can achieve three-dimensional motion. Therefore, the sampling element 310 can move to the position of the sample tube 210, allowing it to aspirate the sample from the tube. Similarly, the sampling element 310 can also move to the position of the reagent kit 220, allowing it to aspirate the sample from the tube. Thus, samples and reagents can be aspirated using the same sampling element 310.

[0049] In some embodiments, the immunoassay analyzer 10 may further include a cleaning tank. Since reagents and samples are aspirated through the same sampling element 310, to avoid cross-contamination, after the sampling element 310 has aspirated the sample, it can be moved to the cleaning tank for cleaning. This also prevents sample adhering to the sampling element 310 from contaminating the reagents when the sample element 310 aspirates them. Similarly, after the sampling element 310 has aspirated the reagents, it can be moved to the cleaning tank for cleaning. This also prevents reagent adhering to the sampling element 310 from contaminating the samples when the sample element 310 aspirates them.

[0050] See Figure 2 , Figure 3 , Figure 4 and Figure 6In some embodiments, the transfer mechanism 400 may include a gripper 410, a first transfer drive module 421, a second transfer drive module 422, and a third transfer drive module 423. The first transfer drive module 421 extends along a first direction, the second transfer drive module 422 extends along a second direction, and the third transfer drive module extends along a third direction. The second transfer drive module 422 is slidably connected to the first transfer drive module, and the first transfer drive module 421 drives the second transfer drive module 422 to reciprocate along the first direction. The third transfer drive module 423 is slidably connected to the second transfer drive module 422, and the second transfer drive module 422 drives the third transfer drive module 423 to reciprocate along the second direction. The gripper 410 is slidably disposed on the third transfer drive module 423, and the third transfer drive module 423 drives the gripper 410 to reciprocate along the third direction. Therefore, when the first transfer drive module 421, the second transfer drive module 422, and the third transfer drive module 423 move, the gripper 410 can move linearly relative to the housing 840 along the first, second, and third directions. This gives the gripper 410 three degrees of freedom in linear motion, thus realizing the three-dimensional motion of the gripper 410. Through the three-dimensional motion of the gripper 410, the gripper 410 can transfer the reactor 20 between different mechanisms.

[0051] See Figure 3 , Figure 4 and Figure 5 In some embodiments, the incubation mechanism 500 also serves to support the reactor 20. When the sampling member 310 adds the sample and reagents to the reactor 20, the liquid formed by the mixture of the sample and reagents can be referred to as the reaction solution. For the reactor 20 containing the reaction solution, the incubation mechanism 500 can heat the reaction solution within the reactor 20 at a constant temperature, thereby allowing the reaction solution to undergo a sufficient chemical reaction under constant temperature conditions. In fact, since the reagents contain solid magnetic beads, the reaction solution is actually a suspension. After the sample and reagents undergo a sufficient chemical reaction, the useful substance (the substance to be analyzed) will combine with the magnetic beads to form a complex. This complex can be used as a measurement object in subsequent testing and analysis processes, thus enabling the detection of different test items on the sample.

[0052] See Figure 3 , Figure 4 and Figure 5In some embodiments, the incubation mechanism 500 can be arranged around the input component 110, which can be understood as the incubation mechanism 500 being sleeved outside the input component 110, and the incubation mechanism 500 can be fixedly arranged relative to the chassis 840. An installation groove can be formed on the incubation mechanism 500, and the end of the inlet component 120 cooperates with the installation groove. On the one hand, the incubation mechanism 500 supports the inlet component 120; on the other hand, it allows the end of the input slide 121 to communicate with one of the slots 111. For other slots 111 that are not connected to the input slide 121, the incubation mechanism 500 will block the openings of the slots 111 facing the incubation mechanism 500. During the rotation of the input component 110, this effectively prevents the reactor 20 from falling out of the slots 111 under centrifugal force, thereby improving the reliability of the input component 110 in buffering and conveying the reactor 20. By arranging the incubation mechanism 500 around the input component 110, the space occupied by both the incubation mechanism 500 and the input component 110 can be reasonably reduced, making the immunoassay analyzer 10 more compact in structure and effectively reducing its size. Simultaneously, the incubation mechanism 500 can be fully utilized to seal the opening of the slot 111 to prevent the reactor 20 from falling out of the slot 111, eliminating the need for other additional sealing components, thereby simplifying the structure of the immunoassay analyzer 10 and reducing manufacturing costs.

[0053] See Figure 6 , Figure 7 , Figure 8 and Figure 9 In some embodiments, the magnetic collection mechanism 600 has a pipetting station 610 and an injection station 620, which are spaced apart along a second direction. The magnetic collection mechanism 600 includes a magnetic collection assembly 640 and a belt drive assembly 650. The magnetic collection assembly 640 carries the reactor 20, and the belt drive assembly 650 drives the magnetic collection assembly 640 to move linearly via a synchronous belt, causing the magnetic collection assembly 640 to reciprocate linearly between the pipetting station 610 and the injection station 620 along the second direction. When the reactor 20 is at the pipetting station 610, the reaction liquid can be injected into the reactor 20.

[0054] In some embodiments, the magnetic collection mechanism 600 further includes a transfer station 630. The pipetting station 610, the injection station 620, and the transfer station 630 are spaced apart along the movement direction of the magnetic collection assembly 640, i.e., the pipetting station 610, the injection station 620, and the transfer station 630 are arranged in a straight line along a second direction. The injection station 620 may be located between the pipetting station 610 and the transfer station 630. The reactor 20 can move into or out of the entire magnetic collection mechanism 600 at the transfer station 630. In other embodiments, the transfer station 630 may be omitted, for example, making the pipetting station 610 and the injection station 620 function as the transfer station 630.

[0055] In some embodiments, the magnetic collection assembly 640 includes a support member 641 and a magnetic member 642. The support member 641 supports the reactor 20, and the magnetic member 642 is fixedly mounted on the support member 641. The support member 641 has a collection position 643 and a sample addition position 644. Both the collection position 643 and the sample addition position 644 may have insertion holes, into which the reactor 20 can be inserted, thereby realizing the supporting function of the support member 641 on the reactor 20. Alternatively, clamping bodies may be provided at the collection position 643 and the sample addition position 644, allowing the reactor 20 to be fixed, thus also realizing the supporting function of the support member 641 on the reactor 20. The sample addition position 644 and the collection position 643 are spaced apart along the movement direction of the magnetic collection assembly 640, that is, the spacing direction of the sample addition position 644 and the collection position 643 is consistent with the movement direction of the support member 641.

[0056] In some embodiments, the number of sample application positions 644 is not less than two, and the number of collection positions 643 is greater than or equal to the number of sample application positions 644. For example, the number of sample application positions 644 can be two, and the number of collection positions 643 can be four, etc. By having multiple sample application positions 644 and collection positions 643, the carrier 641 can simultaneously carry multiple reactors 20 for cleaning and separation operations, thereby improving the working efficiency of the immunoassay analyzer 10. For example, when there are four collection positions 643, the four collection positions 643 are respectively denoted as the first collection position 6431, the second collection position 6432, the third collection position 6433, and the fourth collection position 6434. According to the movement direction of the magnetic collection component 640, the first collection position 6431, the second collection position 6432, the third collection position 6433, and the fourth collection position 6434 are arranged sequentially, with the fourth collection position 6434 being closest to the sample application position 644 and the first collection position 6431 being furthest from the sample application position 644.

[0057] A groove 6411 can be formed on the support member 641. Multiple grooves 6411 are spaced apart along the movement direction of the magnetic collection assembly 640. The grooves 6411 can be positioned between two adjacent collection positions 643. A magnetic element 642 can be fixed within the groove 6411. When two adjacent collection positions 643 located on either side of the groove 6411 contain a reactor 20, the magnetic element 642 can generate a magnetic attraction force on the material within the reactor 20 in both collection positions 643. This allows two collection positions 643 to share a single magnetic element 642, avoiding the need for a separate magnetic element 642 for each collection position 643, thus reducing the number of magnetic elements used and consequently lowering the manufacturing cost of the immunoassay analyzer 10. Furthermore, by housing the magnetic element 642 within the groove 6411, the magnetic element 642 can fully utilize the space within the groove 6411, thereby reducing the overall space occupied by the magnetic collection assembly 640 and consequently reducing the volume of the immunoassay analyzer 10.

[0058] In some embodiments, a groove 6411 on the support member 641 is disposed on the side of the corresponding collection position 643, and a magnetic member 642 is fixed in the groove 6411. When a reactor 20 is present in the collection position 643 corresponding to the groove 6411, the magnetic member 642 generates a magnetic attraction force on the substance in the reactor 20 in the corresponding collection position 643. The magnetic member 642 being disposed on the side of the corresponding collection position 643 can shorten the length of the magnetic collection assembly 640 along the direction of movement, reduce the space occupied by the entire magnetic collection assembly 640, and thus reduce the volume of the immunoassay analyzer 10.

[0059] See Figure 9 In some embodiments, the injection mechanism 700 may include a first injection assembly 710 and a second injection assembly 720, with two injection stations 620, designated as the first injection station 621 and the second injection station 622. When the magnetic collection assembly 640 moves the reactor 20 to the first injection station 621, the first injection assembly 710 injects cleaning fluid into the reactor 20 located at the first injection station 621. When the magnetic collection assembly 640 moves the reactor 20 to the second injection station 622, the second injection assembly 720 injects substrate liquid into the reactor 20 located at the second injection station 622. In other embodiments, the first injection station 621 and the second injection station 622 may be the same station, and the first injection assembly 710 and the second injection assembly 720 may also be the same injection assembly.

[0060] See Figure 4In some embodiments, the mixing mechanism 810 can mix the liquid in the reactor 20. For example, the mixing mechanism 810 can cause the reactor 20 to oscillate through eccentric oscillation, thereby mixing the liquid in the reactor 20. The mixing mechanism 810 has at least two mixing positions, and insertion holes can be opened at the mixing positions. The reactor 20 can be inserted into the insertion holes, so that the mixing positions can support the reactor 20. Since there can be multiple mixing positions, the mixing mechanism 810 can mix the liquid in multiple reactors 20 simultaneously, thereby improving the working efficiency of the mixing mechanism 810 and the entire immunoassay analyzer 10. The number of mixing mechanisms 810 can be one, thus reducing the number of mixing mechanisms 810 used, thereby simplifying the structure of the immunoassay analyzer 10, and ultimately reducing the manufacturing cost and size of the immunoassay analyzer 10.

[0061] See Figure 4 In some embodiments, the measuring mechanism 830 includes a rotatably mounted measuring element. The reactor 20 can be supported on the measuring element, which can rotate about a third-direction axis. When the measuring element rotates the reactor 20 to one designated position, the measuring mechanism 830 can measure the reactor 20. When the measuring element rotates the reactor 20 to another designated position, the reactor 20 to be measured can be moved into the measuring element, and the reactor 20 after measurement can be moved out of the measuring element. The input element 110 also rotates about a third-direction axis, such that the axes around which the measuring element and the input element 110 rotate are parallel to each other. The rotation of the measuring element and the input element 110 can be driven by the same belt drive mechanism; however, the rotational speeds of the measuring element and the input element 110 can be different. This allows the measuring element and the input element 110 to share a single belt drive mechanism, thus avoiding the need for separate drive mechanisms for the measuring element and the input element 110. This reduces the number of drive mechanisms, thereby lowering the manufacturing cost of the immunoassay analyzer 10 and making the immunoassay analyzer 10 more compact and smaller in size.

[0062] See Figure 3 and Figure 4 In some implementations, the output mechanism 820 is equipped with an output slide 821, which is used to arrange and transport the reactor 20. The extension direction of the output slide 821 is set at an acute angle to the third direction. After the reactor 20 has been measured on the measuring mechanism 830, the gripper 410 can place the measured reactor 20 into the output slide 821. Under the action of the reactor 20's own weight, the reactor 20 will slide in the output slide 821, thereby allowing the reactor 20 to be transported to the designated collection container through the output slide 821.

[0063] In some embodiments, in the second direction, the sampling mechanism 300 and the transfer mechanism 400 are located on opposite sides of the movement trajectory of the magnetic collection assembly 640. This avoids collisions and interference between the gripper 410 and the sampling element 310 during movement, thereby improving the reliability and safety of the immunoassay analyzer 10. Furthermore, it allows for a more efficient layout of the sampling mechanism 300 and the transfer mechanism 400, improving the space utilization of the immunoassay analyzer 10 and ultimately reducing its footprint and volume.

[0064] See Figure 3 and Figure 4 The sampling mechanism 300, the liquid injection mechanism 700, and the consumable mechanism 200 are located on the same side of the movement trajectory of the magnetic collection component 640, while the input mechanism 100, the incubation mechanism 500, the mixing mechanism 810, the measuring mechanism 830, the output mechanism 820, and the transfer mechanism 400 are located on the same side of the movement trajectory of the magnetic collection component 640. In other words, in the first direction, the sampling mechanism 300, the liquid injection mechanism 700, and the consumable mechanism 200 are located on one side of the movement trajectory of the magnetic collection component 640; the input mechanism 100, the incubation mechanism 500, the mixing mechanism 810, the measuring mechanism 830, the output mechanism 820, and the transfer mechanism 400 are located on the other side of the movement trajectory of the magnetic collection component 640. This further improves the space utilization of the immunoassay analyzer 10, ultimately reducing the space and volume occupied by the immunoassay analyzer 10.

[0065] See Figure 4 , Figure 7 , Figure 8 and Figure 9 The working principle of the immunoassay analyzer 10 is described below. This working principle can be understood as a method for performing immunoassay on the reactor 20. This working principle can be broken down into the following working steps:

[0066] In the first step, the gripper 410 moves in a three-dimensional linear motion, clamping the empty reactor 20 and causing the magnetic collection assembly 640 to move. This causes a sample loading position 644 on the magnetic collection assembly 640 to move to the transfer station 630. The gripper 410 places the empty reactor 20 from the transfer station 630 into the sample loading position 644 of the magnetic collection assembly 640, and then the gripper 410 moves away from the transfer station 630.

[0067] In the second step, the magnetic collection component 640 drives the empty reactor 20 to the pipetting station 610, and the sampling component 310 moves and adds the sample and reagent to the reactor 20 at the pipetting station 610. At this time, the reactor 20 is filled with the reaction solution formed by the sample and reagent.

[0068] For example, sampling device 310 can first draw a sample from sample tube 210 and add the sample to reactor 20. Then, sampling device 310 can move to a cleaning tank to clean its inner and outer surfaces. After cleaning, sampling device 310 draws reagent from reagent kit 220 and adds the reagent to reactor 20. Alternatively, sampling device 310 can first draw a sample from sample tube 210, then move the sample-bearing sampling device 310 to a cleaning tank to clean its outer surface. Next, sampling device 310 draws reagent from reagent kit 220, then simultaneously adds the sample and reagent to reactor 20, and finally moves to a cleaning tank to clean its inner and outer surfaces. For example, the sampling device 310 can first draw reagents from the reagent kit 220 and add the reagents to the reactor 20, and then move the sampling device 310 to the cleaning tank to clean the inner and outer surfaces of the sampling device 310. After cleaning, the sampling device 310 draws samples from the sample tube 210 and adds the samples to the reactor 20.

[0069] In the third step, the magnetic collection component 640 moves the reactor 20 containing the reaction liquid to the transfer station 630. The gripper 410 moves to the transfer station 630 and moves the reactor 20 out of the magnetic collection component 640 from the transfer station 630. Then, the reactor 20 is moved into the mixing mechanism 810, so that the mixing mechanism 810 mixes the reaction liquid in the reactor 20 so that the reaction liquid can react fully.

[0070] The fourth step involves moving the gripper 410 and removing the reactor 20, after mixing, from the mixing mechanism 810. Then, the reactor 20 is moved into the incubation mechanism 500, whereby the incubation mechanism 500 heats the reaction liquid in the reactor 20 at a constant temperature for a certain period of time, allowing the useful substances in the reaction liquid to fully combine with the magnetic beads to form a bound substance.

[0071] The fifth step is illustrated using the magnetic collection component 640 having a first collection position 6431, a second collection position 6432, a third collection position 6433, and a fourth collection position 6434 as an example. The gripper 410 moves and removes the reactor 20 after incubation from the incubation mechanism 500, causing the magnetic collection component 640 to move the first collection position 6431 to the transfer station 630. Then, the gripper 410 places the reactor 20 from the transfer station 630 into the first collection position 6431.

[0072] In the sixth step, the magnetic collection component 640 moves the reactor 20 at the first collection position 6431 to the first liquid injection station 621. The first liquid injection component 710 injects cleaning liquid into the reactor 20 at the first liquid injection station 621, so that the cleaning liquid comes into contact with the bound material on the magnetic beads, thereby cleaning the bound material to a certain extent.

[0073] In the seventh step, the magnetic collection component 640 moves the first collection position 6431 to the transfer station 630, the gripper 410 moves and moves the reactor 20 from the first collection position 6431 at the transfer station 630, then the magnetic collection component 640 moves the second collection position 6432 to the transfer station 630, and then the gripper 410 moves and moves the reactor 20 from the transfer station 630 into the second collection position 6432.

[0074] In the eighth step, due to the magnetic field generated by the magnetic component 642, the magnetic beads will migrate in the reaction liquid under the influence of the magnetic component 642 until they are adsorbed onto the inner wall surface of the reactor 20. This causes the binding material attached to the magnetic beads to also be adsorbed onto the inner wall surface of the reactor 20. Generally, it takes 10 to 90 seconds to adsorb all the magnetic beads onto the inner wall surface of the reactor 20.

[0075] In other embodiments, the cleaning solution may not be added to the reaction solution, thus the sixth step can be omitted, allowing direct access from the fifth step to the seventh step. Therefore, before removing the waste liquid from the reactor 20 containing the reaction solution, the reactor 20 can be moved to the liquid injection station 620 to inject the cleaning solution, or the cleaning solution may not be injected into the reactor 20.

[0076] In the ninth step, the magnetic collection component 640 moves the second collection position 6432 to the liquid transfer station 610. The sampling component 310 moves and completely absorbs the waste liquid in the reactor 20. Then, the sampling component 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling component 310 to prevent the waste liquid remaining in the sampling component 310 from causing cross-contamination.

[0077] In the tenth step, the magnetic collection component 640 moves the second collection position 6432 to the first liquid injection station 621, and the first liquid injection component 710 injects cleaning liquid into the reactor 20 located at the first liquid injection station 621.

[0078] In the eleventh step, the magnetic collection component 640 moves the second collection position 6432 to the transfer station 630. The gripper 410 moves and moves the reactor 20 from the transfer station 630 out of the magnetic collection component 640. Then, the reactor 20 is moved into the mixing mechanism 810. The mixing mechanism 810 mixes the suspension formed by the cleaning liquid and the magnetic beads. During the mixing process, the cleaning liquid cleans the aggregates attached to the magnetic beads.

[0079] In the twelfth step, the gripper 410 moves and moves the reactor 20 after mixing out of the mixing mechanism 810, so that the magnetic collection component 640 drives the third collection position 6433 to the transfer station 630, and the gripper 410 puts the reactor 20 from the transfer station 630 into the third collection position 6433.

[0080] Step 13: Due to the magnetic field generated by the magnetic component 642, the magnetic beads will migrate in the reaction liquid under the influence of the magnetic component 642 until they are adsorbed onto the inner wall surface of the reactor 20. This causes the binding material on the magnetic beads to also be adsorbed onto the inner wall surface of the reactor 20. Generally, it takes 10 to 90 seconds to adsorb all the magnetic beads onto the inner wall surface of the reactor 20.

[0081] In the fourteenth step, the magnetic collection component 640 drives the third collection position 6433 to the liquid transfer station 610. The sampling component 310 moves and absorbs all the waste liquid in the reactor 20. Then, the sampling component 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling component 310 to prevent the waste liquid remaining in the sampling component 310 from causing cross-contamination.

[0082] At this point, steps 10 to 14 can be considered as a first-stage cleaning and separation process, that is, the process from the injection of cleaning liquid to the removal of waste liquid after the reaction liquid has been completely absorbed from reactor 20 is recorded as a first-stage cleaning and separation process. In the presence of step 6, steps 6 to 9 can be considered as a pre-cleaning and separation process. The entire cleaning and separation process of a reactor 20 can include multiple stages of cleaning and separation. When the cleaning and separation process includes three or more stages of cleaning and separation, steps 10 to 14 can be cycled multiple times. For example, if the entire cleaning and separation process includes two stages of cleaning and separation, steps 10 to 14 will become the first-stage cleaning and separation process. When the entire cleaning and separation process includes two stages of cleaning and separation, steps 10 to 14 can be cycled again. Specifically, the working principle of the immunoassay analyzer 10 can be further broken down into the following steps:

[0083] In the fifteenth step, the magnetic collection component 640 drives the third collection position 6433 to the first liquid injection station 621, and the first liquid injection component 710 injects cleaning liquid into the reactor 20 located at the first liquid injection station 621.

[0084] In the sixteenth step, the magnetic collection component 640 moves the third collection position 6433 to the transfer station 630. The gripper 410 moves and moves the reactor 20 from the transfer station 630 out of the magnetic collection component 640. Then the reactor 20 is moved into the mixing mechanism 810. The mixing mechanism 810 mixes the suspension formed by the cleaning liquid and the magnetic beads. During the mixing process, the cleaning liquid cleans the aggregates attached to the magnetic beads.

[0085] In the seventeenth step, the gripper 410 moves and moves the reactor 20 after mixing out of the mixing mechanism 810, so that the magnetic collection component 640 drives the fourth collection position 6434 to the transfer station 630, and the gripper 410 puts the reactor 20 from the transfer station 630 into the fourth collection position 6434.

[0086] Step 18: Due to the magnetic field generated by the magnetic component 642, the magnetic beads will migrate in the reaction liquid under the influence of the magnetic component 642 until they are adsorbed onto the inner wall surface of the reactor 20. This causes the binding material attached to the magnetic beads to also be adsorbed onto the inner wall surface of the reactor 20. Generally, it takes 10 to 90 seconds to adsorb all the magnetic beads onto the inner wall surface of the reactor 20.

[0087] In the nineteenth step, the magnetic collection component 640 drives the fourth collection position 6434 to the liquid transfer station 610. The sampling component 310 moves and absorbs all the waste liquid in the reactor 20. Then, the sampling component 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling component 310 to prevent the waste liquid remaining in the sampling component 310 from causing cross-contamination.

[0088] After step nineteen is completed, that is, after reactor 20 finishes its final waste liquid extraction, the entire effective cleaning and separation process of the magnetic beads and the aggregates is essentially finished. At this point, steps fifteen through nineteen become the second-stage cleaning and separation treatment.

[0089] In the twentieth step, the magnetic collection component 640 drives the fourth collection position 6434 to the second liquid injection station 622, and the second liquid injection component 720 injects the substrate liquid into the reactor 20 located at the second liquid injection station 622.

[0090] In the twenty-first step, the magnetic collection component 640 drives the fourth collection position 6434 to the transfer station 630. The gripper 410 moves and moves the reactor 20 from the transfer station 630 out of the magnetic collection component 640. Then the reactor 20 is moved into the mixing mechanism 810, which mixes the suspension formed by the substrate liquid, magnetic beads and conjugate.

[0091] In the twenty-second step, the gripper 410 moves and removes the reactor 20, which has been mixed, from the mixing mechanism 810, and then moves the reactor 20 into the incubation mechanism 500 so that the incubation mechanism 500 can heat the liquid in the reactor 20 at a constant temperature for a certain period of time.

[0092] Step 23: The gripper 410 moves and removes the reactor 20 after incubation from the incubation mechanism 500, and moves the reactor 20 into the measuring mechanism 830 for measurement.

[0093] Step 24: After the reactor 20 has been measured, the gripper 410 moves and transfers the measured reactor 20 to the output slide 821 of the output mechanism 820, so that the measured reactor 20 is transported to the collection container through the output slide 821.

[0094] In other embodiments, the gripper 410 can directly move the reactor 20 containing the substrate liquid to the measuring mechanism 830 for measurement after mixing, thereby omitting step 23 and proceeding directly from step 22 to step 24. Therefore, before measuring the reactor 20 containing the substrate liquid, depending on the actual needs, the reactor 20 can be transferred to the incubation mechanism 500 for incubation, or it can be left untouched.

[0095] If different aspiration needles are used for sample injection, reagent injection, and waste liquid aspiration, this will increase the number of aspiration needles required and the number of driving sources needed to move them, thus increasing the manufacturing cost and size of the immunoassay analyzer 10. Furthermore, given the movement of multiple aspiration needles, controlling their movement will be more difficult to minimize interference.

[0096] Regarding the immunoassay analyzer 10 in the above embodiments, since the same sampling element 310 is used for sample injection, reagent injection, and waste liquid aspiration, the number of sampling elements 310 and the number of driving sources for moving the sampling elements 310 are reduced, thereby reducing the manufacturing cost and size of the immunoassay analyzer 10. It also reduces the difficulty of motion control for a single sampling element 310.

[0097] If the magnetic collection component 640 adopts a rotary disc design, the disc rotates to move the reactor 20 to different positions to complete the cleaning and separation. This would increase the size of the disc, as well as the number of aspiration needles and their associated drive sources, thus increasing the manufacturing cost and size of the immunoassay analyzer 10. Furthermore, the increased weight and size of the disc make motion control more difficult, potentially affecting its accuracy and increasing the failure rate.

[0098] Regarding the immunoassay analyzer 10 in the above embodiments, since the magnetic collection component 640 moves linearly between the transfer station 630, the injection station 620, and the pipetting station 610, the difficulty of controlling the linear movement is significantly reduced. This improves the accuracy of the magnetic collection component 640's movement and reduces the failure rate, while also reducing the size of the magnetic collection component 640, thereby achieving a miniaturized design of the immunoassay analyzer 10. Simultaneously, the arrangement direction of the sample application position 644 and the collection position 643 on the magnetic collection component 640 is the same as the direction of movement of the magnetic collection component 640. This effectively avoids the situation where the arrangement direction of the sample application position 644 and the collection position 643 on the magnetic collection component 640 is perpendicular to the direction of movement of the magnetic collection component 640. This results in a long and narrow shape for the magnetic collection component 640, reducing the volume of the magnetic collection component 640 and the entire immunoassay analyzer 10, and also reducing the space covered by the magnetic collection component 640 during movement, further reducing the volume of the immunoassay analyzer 10 and achieving a miniaturized design of the immunoassay analyzer 10.

[0099] If different mixing mechanisms 810 are used for mixing the reaction solution formed by reagents and samples, mixing the washing solution and magnetic beads, and mixing the substrate solution and magnetic beads, the number of mixing mechanisms 810 used will increase, thereby increasing the manufacturing cost and size of the immunoassay analyzer 10.

[0100] As for the immunoassay analyzer 10 in the above embodiments, since the same mixing mechanism 810 is used for mixing the reaction solution, the washing solution and the magnetic beads, and the substrate solution and the magnetic beads, the number of mixing mechanisms 810 used will be greatly reduced, thereby reducing the manufacturing cost of the immunoassay analyzer 10 and realizing the miniaturization design of the immunoassay analyzer 10.

[0101] Because the immunoassay analyzer 10 has low manufacturing cost, small size, and easy motion control, the failure rate of the immunoassay analyzer 10 can be reduced, and the professional requirements for its operation can be lowered, thus enabling the immunoassay analyzer 10 to be widely used in small and medium-sized laboratories or medical institutions.

[0102] Given that there are multiple collection points 643, for two adjacent collection points 643, the reactor 20 is injected with cleaning solution at one collection point 643 and then transferred to the other collection point 643 to collect waste liquid. This allows the magnetic collection assembly 640 to simultaneously support multiple reactors 20 for cleaning and separation. That is, reactors 20 that have completed cleaning and separation can be sequentially removed from the magnetic collection assembly 640, while reactors 20 requiring cleaning and separation can be sequentially moved into the magnetic collection assembly 640. This improves the working efficiency of the immunoassay analyzer 10 to a certain extent.

[0103] Since the sampling element 310 can perform three-dimensional linear motion, it has three degrees of freedom. The fixed support assembly holding the reaction liquid allows the sampling element 310 to draw the reaction liquid from the support assembly through three-dimensional linear motion. Similarly, since the gripper 410 can also perform three-dimensional linear motion, it has three degrees of freedom. The input element 110 can rotate, ensuring that the gripper 410 transfers the reactor from the input element 110 through three-dimensional linear motion. Therefore, this design simplifies the structure of the immunoassay analyzer 10, thereby reducing its manufacturing cost and size.

[0104] See Figure 11 By operating the immunoassay analyzer 10, the following immunoassay method can be formed, which can be understood as the immunoassay method being executed by the immunoassay analyzer 10. The sampling element 310 and the gripper 410 move linearly along a first direction, a second direction, and a third direction that are set at an angle to each other; the input element 110 rotates around a rotating axis extending along the third direction; and the magnetic collection assembly 640 carries the reactor 20 and moves linearly between the pipetting station 610 and the injection station 620, which are spaced apart along the first direction. Based on this, the immunoassay method can mainly consist of the following steps:

[0105] S910, the empty reactor 20 is transferred to the magnetic collection assembly 640 by the gripper 410. The magnetic collection assembly 640 carries the empty reactor 20 to the pipetting station 610. The sample and reagent are injected into the reactor 20 by the sampling device 310. The reactor 20 is then removed from the magnetic collection assembly 640 by the gripper 410 to mix the liquid in the reactor 20.

[0106] S920, the well-mixed reactor 20 is incubated;

[0107] S930, the incubated reactor 20 is moved into the magnetic collection assembly 640 and then to the liquid transfer station 610 by the gripper 410, the magnetic beads in the reactor 20 are collected on the inner wall of the reactor 20, and the waste liquid is taken out by the sampling component 310.

[0108] S940, the magnetic collection component 640 carries the reactor 20 after absorbing the waste liquid to the liquid injection station 620, the first liquid injection component 70 injects cleaning liquid into the reactor 20, and the gripper 410 moves the reactor 20 out of the magnetic collection component 640 to mix the liquid in the reactor 20.

[0109] S950, the reactor 20 containing the cleaning liquid is moved into the magnetic collection assembly 640 and moved to the liquid transfer station 610 by the gripper 410, and the waste liquid is sucked up by the sampling element 310;

[0110] S960, the magnetic collection assembly 640 carrying the reactor 20 is moved to the liquid injection station 620, and the substrate liquid is injected into the reactor 20 through the second liquid injection assembly 720; and

[0111] S970, the reactor 20 containing the substrate liquid is transferred to the measuring mechanism 830 for measurement via the gripper 410.

[0112] For the other steps of the immunoassay method, please refer to the above description of the structure and working principle of the immunoassay analyzer, which will not be repeated here.

[0113] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0114] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. An immunoassay method, characterized in that, The sampling component and gripper move linearly along a first, second, and third direction arranged at angles to each other; the magnetic collection assembly carries the reactor and moves linearly between pipetting and injection stations spaced apart along the second direction; the immunoassay method includes the following steps: An empty reactor is transferred to the magnetic collection assembly by a gripper. The magnetic collection assembly carries the empty reactor to the pipetting station. Samples and reagents are added to the reactor through the sampling device. The reactor is then removed from the magnetic collection assembly by the gripper to mix the liquid in the reactor. The mixed reactor was incubated. The gripper moves the incubated reactor into the magnetic collection assembly and then to the liquid transfer station, where magnetic beads are collected on the inner wall of the reactor, and waste liquid is extracted through the sampling device. The reactor, after being sucked up by the magnetic collection component, is moved to the liquid injection station. Cleaning liquid is injected into the reactor through the first liquid injection component, and the reactor is moved out of the magnetic collection component by the gripper to mix the liquid in the reactor. The reactor containing the cleaning solution is moved into the magnetic collection assembly by the gripper and then moved to the liquid transfer station, where the waste liquid is drawn up by the sampling element. The magnetic collection assembly carries the reactor to the injection station, and the substrate liquid is injected into the reactor through the second injection assembly; and The reactor containing the substrate liquid is transferred to the measuring mechanism for measurement using the gripper; The magnetic collection assembly has a sample addition position and a collection position for carrying the reactor. The sample addition position and the collection position are spaced apart along the movement direction of the magnetic collection assembly. The sample addition position can move to the pipetting station, and the collection position can move to the pipetting station or the injection station. The number of collection locations is multiple. For two adjacent collection locations, the reactor injects cleaning liquid into one collection location and then moves to the other collection location to collect waste liquid.

2. The immunoassay method according to claim 1, characterized in that, The liquid in the reactor is mixed using the same mixing mechanism.

3. The immunoassay method according to claim 1, characterized in that, After the sampling device is injected with a sample, reagent, or waste liquid, it is cleaned.

4. The immunoassay method according to claim 1, characterized in that, The step of removing the waste liquid after the reactor has been completely aspirated from the reaction solution and then injecting the cleaning solution is referred to as the first-stage cleaning and separation process. The immunoassay method includes multiple stages of the cleaning and separation process.

5. The immunoassay method according to claim 1, characterized in that, The number of injection stations is two, and the two injection stations are respectively designated as the first injection station and the second injection station. Cleaning liquid is injected into the reactor at the first injection station; after the reactor has absorbed the waste liquid for the last time, substrate liquid is injected into the reactor at the second injection station.

6. The immunoassay method according to claim 1, characterized in that, Before the reactor containing the reaction liquid is drained of waste liquid, the reactor is moved to the liquid injection station to inject cleaning liquid.

7. The immunoassay method according to claim 1, characterized in that, Between the injection of the substrate liquid into the reactor and the measurement, the reactor is removed from the magnetic collection assembly by a gripper for incubation.

8. The immunoassay method according to claim 1, characterized in that, The reactor is carried out through a fixed incubation mechanism.

9. The immunoassay method according to claim 1, characterized in that, The number of sampling locations is two.

10. The immunoassay method according to claim 1, characterized in that, The number of collection locations is four.

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