Wide-field apochromatic microscope objective with low back focal length and method for determining the same

Abstract

The present application relates to the field of optical imaging technology, and particularly relates to a low-telecentricity wide-field apochromatic microscope objective and a determination method thereof. The microscope objective comprises coaxially arranged multiple spherical lenses, the multiple spherical lenses comprising, from the object side to the image side, a telecentricity lens group, an aperture lens group, a chromatic aberration lens group and an aberration lens group; the telecentricity lens group is used to expand the imaging field of view and the front and rear depth of field of the observation object, and comprises a first lens and a first lens group; the aperture lens group is used to enlarge the observation object to the target aperture range, and comprises a second lens group; the chromatic aberration lens group is used to correct chromatic aberration, and comprises a third lens group, a fourth lens group and a fifth lens group; the aberration lens group is used to correct aberration, and comprises a sixth lens group, a fifteenth lens and a seventh lens group. The problem that the telecentricity, numerical aperture and object field cannot meet the requirements and the imaging error is large when performing gel surface bacterial imaging analysis is solved.

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