Data reading method, reading system and gene sequencing system of semiconductor sequencing chip

Abstract

The application discloses a data reading method and system of a semiconductor sequencing chip. The data reading method of the semiconductor sequencing chip comprises the following steps: controlling the semiconductor sequencing chip to be immersed in a reagent; reading data output by the semiconductor sequencing chip at least once when at least one core row is immersed each time until all core rows of the semiconductor sequencing chip are immersed in the reagent. According to the data reading method, the data output by the semiconductor sequencing chip is read at least once when at least one core row is immersed each time, the reading frequency is reduced from reading all cores in parallel to reading all core rows, and the system load of sequential reading is reduced by joint control of immersion and reading, so that the transmission, calculation and storage of the system are realized more simply, and the cost is reduced.

Description

Technical Field

[0001] This invention relates to the field of gene sequencing technology, and more specifically, to a data reading method for a semiconductor sequencing chip, a data reading system for a semiconductor sequencing chip, and a gene sequencing system. Background Technology

[0002] The sequencing substrates (also known as semiconductor sequencing chips) used in current high-throughput gene sequencers commonly fall into two categories: surface-mount chips and semiconductor sequencing chip integrated circuits. The former typically captures fluorescence signals through a microscope's optical system, while semiconductor sequencing chips use internal integrated circuits to acquire and convert electrical or optical signals into digital signals.

[0003] Semiconductor sequencing chips are typically fabricated using 6-inch, 8-inch, and 12-inch silicon wafers through front-end and back-end processes such as photolithography and etching. Currently, the most widely used mass production process in the semiconductor industry is the 12-inch wafer. The size of a single chip die is limited by the photolithography mask, so dies on the wafer are usually cut and packaged into multi-core arrays to achieve a single-module, multi-core semiconductor sequencing chip. However, in this approach, the loading of biochemical reagents and the reading of signals become system bottlenecks due to the extremely large amount of data. Summary of the Invention

[0004] This invention provides a data reading method for semiconductor sequencing chips, a reading system, and a gene sequencing system.

[0005] This invention provides a data reading method for a semiconductor sequencing chip used in a gene sequencing system. The semiconductor sequencing chip includes multiple cores arranged in an array to form multiple core rows. The data reading method includes:

[0006] The semiconductor sequencing chip is controlled to be immersed in the reagent in a core behavior immersion unit manner;

[0007] Immerse at least one core row, read data from the semiconductor sequencing chip output at least once, until all core rows are immersed in the reagent.

[0008] In the above data reading method, the data output of the semiconductor sequencing chip is read at least once every time at least one core row is immersed. This reduces the number of reads from reading all cores in parallel to reading all core rows. In other words, by controlling the immersion-reading process together, the system load of sequential reading is reduced, and the transmission, calculation and storage of the system are realized more simply, while also reducing costs.

[0009] In some embodiments, the gene sequencing system includes a row switching and common read unit connected to all core rows, and the data reading method includes:

[0010] The row switching and common reading unit controls the data channel switching and data reading of the core row immersed in the reagent.

[0011] In some embodiments, the semiconductor sequencing chip is immersed in the reagent by a robotic arm, the continuous exposure time of the immersion core row is controlled by a first timing sequence, and the movement time of the robotic arm is controlled by a second timing sequence. In the first timing sequence, a waiting time is separated from each movement time of the robotic arm before and after the continuous exposure time of the immersion core row.

[0012] In some implementations, data transmission into the core rows is controlled by a third timing sequence, wherein the data transmission time of each core row in the third timing sequence follows the continuous exposure time of the corresponding core row.

[0013] In some implementations, each core includes a pixel array, which may be a single pixel array or a pixel array composed of at least two sub-pixel arrays joined together.

[0014] This invention discloses a data reading system for a semiconductor sequencing chip, used in a gene sequencing system. The semiconductor sequencing chip includes multiple cores arranged in an array to form multiple core rows. The data reading system includes: a control device; and a robotic arm connected to the control device. The control device controls the robotic arm to immerse the semiconductor sequencing chip in a reagent. The control device is used to immerse at least one core row and read data output from the semiconductor sequencing chip at least once, until all core rows of the semiconductor sequencing chip are immersed in the reagent.

[0015] In the aforementioned data reading system, the data output of the semiconductor sequencing chip is read at least once every time at least one core row is immersed. This reduces the number of reads by reducing the number of reads from reading all cores in parallel to reading all core rows. In other words, by controlling the immersion-reading process together, the system load of sequential reading is reduced, and the transmission, computation, and storage of the system are realized more simply, while also reducing costs.

[0016] In some embodiments, the control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common readout unit connected to all core rows. The control device is used to control the data channel switching and data readout of the core rows immersed in the reagent through the row switching and common readout unit.

[0017] In some embodiments, the control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common reading unit, which is used to control the time difference between the immersion time of the core row and the data reading time to not exceed a preset range.

[0018] In some embodiments, the control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common readout unit, which controls the continuous exposure time of the core immersion row through a first timing sequence and controls the movement time of the robotic arm through a second timing sequence. In the first timing sequence, a waiting time is separated from each movement time of the robotic arm before and after the continuous exposure time of the core immersion row and from each movement time of the robotic arm in the second timing sequence.

[0019] In some implementations, the line switching and common reading unit is further configured to control the data transmission of the core lines by means of a third timing sequence, wherein the data transmission time of each core line in the third timing sequence is after the continuous exposure time of the corresponding core line.

[0020] A gene sequencing system according to an embodiment of the present invention includes the data reading system of any of the above embodiments.

[0021] The aforementioned gene sequencing system reads the data output from the semiconductor sequencing chip at least once each time at least one core row is immersed. This reduces the number of reads by reading all cores instead of all core rows. In other words, by controlling the immersion-reading process together, the system load of sequential reading is reduced, and the transmission, computation, and storage of the system are realized more simply, while also reducing costs.

[0022] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0023] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0024] Figure 1 This is a flowchart of a data reading method for a semiconductor sequencing chip according to an embodiment of the present invention;

[0025] Figure 2 This is a schematic diagram of the data reading system of the semiconductor sequencing chip according to an embodiment of the present invention;

[0026] Figure 3This is a schematic diagram of the image processing module of the data reading system for the semiconductor sequencing chip according to an embodiment of the present invention;

[0027] Figure 4 This is a timing diagram of data reading according to an embodiment of the present invention;

[0028] Figure 5 This is a schematic diagram of the row-by-row immersion method of the semiconductor sequencing chip according to an embodiment of the present invention;

[0029] Figure 6 This is a schematic diagram of the structure of a semiconductor sequencing chip according to an embodiment of the present invention;

[0030] Figure 7 This is a schematic diagram of the region division of the semiconductor sequencing chip according to an embodiment of the present invention;

[0031] Figure 8 This is a schematic diagram of the core structure of the semiconductor sequencing chip according to an embodiment of the present invention;

[0032] Figure 9 This is another structural schematic diagram of the core of the semiconductor sequencing chip according to an embodiment of the present invention;

[0033] Figure 10 This is another structural schematic diagram of the core of the semiconductor sequencing chip according to an embodiment of the present invention;

[0034] Figure 11 This is a cross-sectional schematic diagram of a multi-core semiconductor sequencing chip according to an embodiment of the present invention;

[0035] Figure 12 A schematic diagram of a data readout circuit in a related technology;

[0036] Figure 13 This is a schematic diagram of the data readout circuit of the semiconductor sequencing chip according to an embodiment of the present invention. Detailed Implementation

[0037] To better understand the above-mentioned objects, features, and advantages of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that many specific details are set forth in the following description to provide a thorough understanding of the present invention; however, the present invention may also be implemented in other ways different from those described herein. Therefore, the scope of protection of the present invention is not limited to the specific embodiments disclosed below.

[0038] Please see Figure 1 This invention provides a data reading method for a semiconductor sequencing chip, used in a gene sequencing system. Please refer to [the relevant documentation]. Figure 6 The semiconductor sequencing chip 14 includes multiple cores 42, which are arranged in an array to form multiple core rows 44.

[0039] Data reading methods include:

[0040] Step 11, please refer to Figure 5 The semiconductor sequencing chip 14 is immersed in the reagent in an immersion manner with core rows 44 as immersion units;

[0041] Step 13: Read the data output from the semiconductor sequencing chip 14 at least once each time at least one core row 44 is immersed in the reagent.

[0042] In the above data reading method, the data output of the semiconductor sequencing chip 14 is read at least once every time at least one core row 44 is immersed. This reduces the number of reads from reading all cores 42 in parallel to reading all core rows 44. In other words, by controlling the immersion-reading process together, the system load of sequential reading is reduced, and the transmission, calculation and storage of the system are realized in a simpler way, which also reduces the cost.

[0043] It should be noted that in the data reading method of this invention, the data output of the semiconductor sequencing chip 14 can be read once every time a core row 44 is immersed, or the data output of the semiconductor sequencing chip 14 can be read once every time multiple core rows 44 are immersed, in order to improve the data reading speed. Alternatively, for devices with slower reading speeds, one core row 44 can be immersed and two data readings can be performed. The number of core rows 44 immersed in the reagent each time and the number of signal readings each time need to be set according to the actual situation, and no specific limitation is made here.

[0044] Please see Figure 2 The data reading system 100 of the semiconductor sequencing chip 14 according to an embodiment of the present invention includes a control device 26 and a robotic arm 10. Reagents can be placed in a reagent tank 12. The robotic arm 10, reagent tank 12, and semiconductor sequencing chip 14 can be placed in a sealed space, where a series of biochemical reactions for gene sequencing are carried out. There can be one or more reagent tanks 12, each containing reagents required for gene sequencing. The biochemical reactions occurring in the reagent tank 12 require maintaining a certain temperature, time, and environment; these conditions are controlled by the control device 26.

[0045] The control device 26 may include a display screen 28 and a control module 30. The display screen 28 may be a touch screen, through which the operator controls the entire gene sequencing process. The control module 30 may include a mechanical control module 16, a fluid control module 18, a temperature control module 20, an environmental control module 22, and an image processing module 24.

[0046] The mechanical control module 16 controls the movement of the robotic arm 10 in holding the semiconductor sequencing chip 14, and controls the semiconductor sequencing chip 14 to be immersed in the reagent in the reagent tank 12 in a core row 44 as the immersion unit. The mechanical control module 16 can control the reaction time of the semiconductor sequencing chip 14 in the reagent tank 12. At the same time, the mechanical control module 16 can control the movement speed of the robotic arm 10 within an appropriate range, which can reduce the amount of reagent carried out by the semiconductor sequencing chip 14 when entering and leaving the reagent tank. At the same time, the appropriate movement speed of the robotic arm 10 can also reduce the amount of bubbles generated in the reagent.

[0047] The fluid control module 18 is used to monitor the reagent content in the reagent tank 12. By detecting the quality of the reagent, the fluid control module 18 can monitor the change in the reagent content in the reagent tank, and then control the relevant water pumps and valves to replenish and circulate the reagent, so as to maintain the content of the key reactants required for the biochemical reaction in the reagent at a certain level.

[0048] The temperature control module 20 is used to monitor and control the temperature in the enclosed space through a temperature sensor to maintain it at a suitable temperature required for the biochemical reaction.

[0049] The environmental control module 22 is used to monitor and control the content of various main gases in the enclosed space, and to ensure that the biochemical reaction takes place in a low-oxygen environment by filling with nitrogen or other means.

[0050] Please see Figure 3 The image processing module 24 can be connected to the semiconductor sequencing chip 14 via the interface board 38. The image processing module 24 includes a line switching and common reading unit 34, a main control board 36, and a driver board 40. The line switching and common reading unit 34 reads the image signals acquired by the semiconductor sequencing chip 14 line by line and transmits them to the back-end hard disk for storage. The line switching and common reading unit 34 can also communicate with the main control board 36 for bidirectional data transmission. The power board 32 supplies power to the image processing module 24, and the main control board 36 performs data collection, processing, and instruction output. The display is connected via the driver board 40 for output.

[0051] In some embodiments, the gene sequencing system includes a row switching and common readout unit 34, which connects all core rows 44, and the data reading method includes:

[0052] The data channel switching and data reading of the core 42 immersed in the reagent are controlled by the row switching and common reading unit 34. In this way, the data of the semiconductor sequencing chip 14 can be read out line by line, reducing the difficulty of data reading, wiring, transmission, buffering and processing by the computer system.

[0053] Specifically, first, the logical relationships between multiple chip cores 42 fabricated on a wafer using a photomask are defined. For an example, please refer to... Figure 6 The system comprises 69 cores 42 arrayed on a semiconductor sequencing chip 14. By processing the circuitry within the cores 42, the 69 cores 42 are divided into 9 core rows 44. One or more cores 42 within each core row 44 simultaneously read data. A row switching and common reading unit 34, including row switching and common reading circuitry, connects to all core rows 44. The image signal is read out row by row through the row switching and common reading unit 34. Under the interaction of the control channel, data is read at least once for each immersion in at least one core row 44.

[0054] In one example, please refer to Figure 7 The entire semiconductor sequencing chip 14 is divided into multiple regions 48 parallel to the reagent reservoir 12. Each region 48 includes a core row 44, and each region 48 is the area where the robotic arm 10 moves once to immerse the semiconductor sequencing chip 14 in reagents for biochemical reactions and data reading. By dividing the chip into several regions 48, the amount of movement and the number of movements of the robotic arm 10 in each operation of the entire semiconductor sequencing chip 14 are determined. Figure 7 In the embodiment shown, the entire semiconductor sequencing chip 14 is divided into 7 regions.

[0055] In other embodiments, the entire semiconductor sequencing chip 14 is divided into multiple regions 48, each region 48 can be multiple core rows 44. By changing its circuit logic, the multiple core rows 44 are combined into one region 48, and biochemical reactions and at least one data reading are performed in a single action of immersion in reagent controlled by the robotic arm 10.

[0056] In one example, by using combined immersion-read control, the system load of sequential reading is reduced to 16% of that of parallel full-wafer (semiconductor sequencing chip 14) reading. Using the above system logic and method, the four bases ATCG can be represented by compressing the 10-bit digital value of each pixel to 2 bits. After processing the data from the first immersion core, the data from all cores of the full wafer (semiconductor sequencing chip 14) is normalized, and the base sequence result can be obtained using a simplified algorithm. Therefore, the system's transmission, computation, and storage are extremely simple to implement, and the cost is very low.

[0057] In some embodiments, a robotic arm 10 controls the immersion of the semiconductor sequencing chip 14 in the reagent. A first timing sequence controls the continuous exposure time of the immersion core row 44, and a second timing sequence controls the movement time of the robotic arm 10. A waiting time is separated between each movement time of the robotic arm 10 and the continuous exposure time of the immersion core row 44 in the first timing sequence. This ensures that the robotic arm 10 remains stationary during the exposure time, resulting in clearer and more accurate image signals.

[0058] Specifically, Figure 4 This is a timing image captured during a specific period of the test. The exposure time is determined by the light intensity and signal-to-noise ratio of the biochemical reaction. The exposure time was obtained through preliminary experimental testing and calculation. The continuous exposure time of the core row 44 is controlled by the first timing sequence. A waiting time is set before and after each exposure time (the high-level part of the first timing sequence). During the two waiting times, the movement time of the robot arm 10 is set by the second timing sequence. By controlling the movement of the robot arm 10 through these three timing sequences, the robot arm 10 is kept in a stationary state during the exposure time, thereby obtaining a clear and accurate image signal.

[0059] In some implementations, data transmission into the core row 44 is controlled by a third timing sequence, where the data transmission time into the core row 44 is after the continuous exposure time of the corresponding core row 44. Thus, data is read after each core row 44 is immersed, achieving joint control of immersion and readout, thereby reducing the system's transmission, buffering, and processing load.

[0060] Specifically, the robotic arm 10 holds the semiconductor sequencing chip 14 and moves it row by row along the direction of the vertical reagent tank 12. In each core row 44, as the robotic arm 10 gradually immerses the reagent and a biochemical reaction occurs, the exposure control is switched to the reagent immersion area 48. The image signal is read out through the row switching and common reading unit 34. The data is transmitted to the data channel step by step through the row switching circuit logic, thereby reducing the transmission, buffering and processing load of the system.

[0061] In some implementations, each core 42 includes a pixel array 51, which may be a single pixel array 51 or a pixel array 51 formed by stitching together at least two sub-pixel arrays 50. This allows for the creation of ultra-large-size, ultra-large-array semiconductor sequencing chips 14, such as... Figure 11 14 is a multi-core spliced ​​semiconductor sequencing chip.

[0062] Specifically, please refer to Figure 8The pixel array 51 occupies the center of the core 42, while the peripheral circuitry, including the phase-locked loop, decoder controller and digital buffer 52, correlated double sampling circuit and comparator 54, readout circuitry 56 (including digital and analog processors, decoder and charge mode readout circuitry), decoder and driver 58, is located around the pixel array 51 or on another wafer to which it will be bonded. Figure 9 As shown. In other embodiments, pixel array 51 can be formed by splicing together several sub-pixel arrays 50. In one example, as Figure 10 As shown, pixel array 51 is obtained by stitching together four sub-pixel arrays 50 using stitching techniques, thereby enabling a larger chip area. In the illustrated embodiment, one pixel array 51 includes r*c pixels.

[0063] In an embodiment of the present invention, the semiconductor sequencing chip 14 may include a data readout circuit 56. The semiconductor sequencing chip 14 of the present invention may use a simplified data readout circuit 56 to reduce cost and complexity.

[0064] Please refer to Figure 12 In related technologies, the data readout circuit 56 includes a color image processing module with functions such as Automatic Exposure Control / Automatic Gain Control Mode (AEC / AGC), Automatic White Balance (AWB), Black Level Compensation (BLC), Defect Pixel Correction (DPC), Lens Shading Correction, Color Interpolation, Color Correction Matrix, Tone Mapping, and JPEG image format encoding.

[0065] In this embodiment of the invention, for the fabrication of the semiconductor sequencing chip 14, please refer to [link / reference needed]. Figure 13 A simplified circuit structure for the data readout circuit 56 can be adopted. For example, the color image processing module that is not needed in the semiconductor sequencing system can be removed. By optimizing the circuit structure, the circuit area can be reduced, additional processing technology can be eliminated, and sequencing costs can be further reduced.

[0066] In summary, the data reading method for the semiconductor sequencing chip 14 provided by this invention divides the entire semiconductor sequencing chip 14 into multiple regions 48 parallel to the reagent slots 12. One or more cores 42 in each row are treated as a core row 44, and data is read simultaneously. Under the interaction of the control channel, each row is read at least once after immersion. Data is transmitted step-by-step to the data channel through a common reading unit logic. Therefore, the transmission, buffering, and processing load of each row is significantly reduced. That is, through the joint control of immersion and reading, the system load is reduced, and the transmission, computation, and storage of the system are realized more simply, while also reducing costs.

[0067] This invention also provides a data reading system 100 for a semiconductor sequencing chip 14, used in a gene sequencing system. The semiconductor sequencing chip 14 includes multiple cores 42 arranged in an array to form multiple core rows 44. The data reading system 100 includes:

[0068] Control device 26;

[0069] The robotic arm 10 is connected to the control device 26, which controls the robotic arm 10 to immerse the semiconductor sequencing chip 14 into the reagent in a core row 44 as the immersion unit.

[0070] The control device 26 is used to read the data output by the semiconductor sequencing chip 14 at least once each time at least one core row 44 is immersed, until all core rows 44 are immersed in the reagent.

[0071] The data reading system 100 of the semiconductor sequencing chip 14 controls the entire gene sequencing process through the control device 26. By combining immersion-reading control, the system load of sequential reading is reduced, and the transmission, calculation and storage of the system are realized in a simpler way, which also reduces the cost.

[0072] In some embodiments, the control device 26 includes an image processing module 24 connected to the semiconductor sequencing chip 14. The image processing module 24 includes a row switching and common reading unit 34, which connects to all core rows 44. The control device 26 controls the switching of data channels and data reading of the core rows 44 immersed in the reagent through the row switching and common reading unit 34. In this way, the data of the semiconductor sequencing chip 14 can be read out row by row, reducing the difficulty of data reading, routing, transmission, buffering, and processing by the computer system.

[0073] Specifically, please refer to Figure 3The semiconductor sequencing chip 14 and the line switching and common reading module 34 are connected through the interface board 38. The line switching and common reading unit 34 may include a line switching and common reading circuit. The line switching and common reading unit 34 is connected to all core lines 44. The image signal is read out line by line through the line switching and common reading unit 34 and transmitted to the back-end hard disk for storage. The synchronous line switching and common reading unit 34 communicates with the main control board 36 to transmit data bidirectionally, realizing the transformation of data reading from synchronous reading to step-by-step reading, which greatly reduces the amount of data transmitted and processed.

[0074] The time difference in data reading time for each core row 44 is controlled to not exceed a preset range. This ensures that the signals received by all core rows 44 are relatively uniform.

[0075] Specifically, the image processing module 24 is set to the same data reading time, and the time difference between the biochemical reaction of each core row 44 immersed in the reagent does not exceed the preset range. The signal is collected at a fixed time after each core row 44, which can ensure that the signals obtained by all core rows 44 are relatively uniform.

[0076] In other embodiments, the data reading time set by the image processing module 24 can be controlled to not exceed a preset time range. The preset time range is determined by the time and preset range during which the core row 44 undergoes a biochemical reaction in the reagent. Within this preset time range, the image processing module 24 collects signals from the immersed portion, ensuring that data can be collected from all core rows 44. For example, if the preset range is 0-1s, and the immersion time of the core row is 10s, then the preset time range for the data reading time is 10s-11s, thus ensuring that the time difference between the immersion time of the core row and the data reading time does not exceed the preset range.

[0077] In some embodiments, the control device 26 includes an image processing module 24 connected to the semiconductor sequencing chip 14. The image processing module 24 includes a row switching and common readout unit 34, which controls the continuous exposure time of the immersion core row 44 via a first timing sequence and the movement time of the robotic arm 10 via a second timing sequence. A waiting time is separated before and after each movement time of the robotic arm 10 in the second timing sequence from the continuous exposure time of the immersion core row 44. Thus, the robotic arm 10 remains stationary during the exposure time, resulting in a clearer and more accurate image signal.

[0078] Specifically, the exposure time is determined by the light intensity and signal-to-noise ratio of the biochemical reaction. The exposure time is obtained through preliminary experimental testing and calculation. The first timing sequence controls the continuous exposure time of the immersion core row 44. A waiting time is set before and after each exposure time. During the two waiting times, the second timing sequence controls the movement time of the robotic arm 10. Through these three timing sequences, the robotic arm 10 is kept in a static state during the exposure time, thereby obtaining a clear and accurate image signal.

[0079] In some implementations, the line switching and common read unit 34 is also used to control the data transmission of the immersed core line 44 via a third timing sequence, in which the data transmission time of the immersed core line 44 is after the continuous exposure time of the corresponding core line 44. In this way, data is read after each core line 44 is immersed, realizing joint control of immersion-read, thereby reducing the transmission, buffering, and processing load of the system.

[0080] Specifically, the robotic arm 10 holds the semiconductor sequencing chip 14 and moves it row by row along the direction of the vertical reagent tank 12. In each core row 44, as the robotic arm 10 gradually immerses the reagent and a biochemical reaction occurs, the exposure control is switched to the reagent immersion area 48. The image signal is read out through the row switching and common reading unit 34. The data is transmitted to the data channel step by step through the row switching circuit logic, thereby reducing the transmission, buffering and processing load of the system.

[0081] In summary, the data reading system for the semiconductor sequencing chip 14, through a series of control modules 30 in the hardware system, realizes the data reading method for the semiconductor sequencing chip 14 disclosed in this invention. In the sequencing module, the robotic arm 10 grips the semiconductor sequencing chip 14 and moves it row by row in a direction perpendicular to the reagent tank 12. Signals are read by the image processing module connected to the semiconductor sequencing chip 14, achieving joint control of the semiconductor sequencing chip 14's immersion and reading. This reduces the system load of sequential reading, simplifies the system's transmission, computation, and storage, and also lowers costs.

[0082] The present invention also provides a gene sequencing system. The above description of the implementation method and system for reading data from the semiconductor sequencing chip 14 and its beneficial effects are also applicable to the gene sequencing system of the present invention. To avoid redundancy, they will not be elaborated in detail here.

[0083] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with an embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0084] Through the above description of the embodiments, those skilled in the art can clearly understand that the methods of the above embodiments can be implemented by means of software plus necessary general-purpose hardware platforms. Of course, they can also be implemented by hardware, but in many cases the former is a better implementation method. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, can be embodied in the form of a software product. This computer software product is stored in a storage medium (such as ROM / RAM, magnetic disk, optical disk) as described above, and includes several instructions to cause a terminal device (which may be a mobile phone, computer, server, air conditioner, or network device, etc.) to execute the methods of the various embodiments of this application.

[0085] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A data reading method for a semiconductor sequencing chip, used in a gene sequencing system, characterized in that, The semiconductor sequencing chip includes multiple cores arranged in an array to form multiple core rows, and the data reading method includes: The semiconductor sequencing chip is controlled to be immersed in the reagent in a core behavior immersion unit manner; At least once each core row is immersed, the data output from the semiconductor sequencing chip is read, until all core rows are immersed in the reagent; The semiconductor sequencing chip is immersed in the reagent by a robotic arm. The continuous exposure time of the immersion core row is controlled by a first timing sequence, and the movement time of the robotic arm is controlled by a second timing sequence. Before and after the continuous exposure time of the immersion core row in the first timing sequence, there is a waiting time between each movement time of the robotic arm in the second timing sequence.

2. The data reading method according to claim 1, characterized in that, The gene sequencing system includes a row switching and common read unit, which connects all core rows. The data reading method further includes: The row switching and common reading unit controls the data channel switching and data reading of the core row immersed in the reagent.

3. The data reading method according to claim 1, characterized in that, The data transmission of the core rows is controlled by a third timing sequence, wherein the data transmission time of each core row in the third timing sequence is after the continuous exposure time of the corresponding core row.

4. The data reading method according to claim 1, characterized in that, Each of the cores includes a pixel array, which may be a single pixel array or a pixel array composed of at least two sub-pixel arrays joined together.

5. A data reading system for a semiconductor sequencing chip, used in a gene sequencing system, characterized in that, The semiconductor sequencing chip includes multiple cores arranged in an array to form multiple core rows; the data reading system includes: Control device; A robotic arm, connected to the control device, is used to control the robotic arm to immerse the semiconductor sequencing chip into the reagent in a core behavior immersion unit manner; The control device is used to read the data output by the semiconductor sequencing chip at least once each time at least one of the core rows is immersed, until all core rows are immersed in the reagent; The semiconductor sequencing chip is immersed in the reagent by a robotic arm. The continuous exposure time of the immersion core row is controlled by a first timing sequence, and the movement time of the robotic arm is controlled by a second timing sequence. Before and after the continuous exposure time of the immersion core row in the first timing sequence, there is a waiting time between each movement time of the robotic arm in the second timing sequence.

6. The data reading system according to claim 5, characterized in that, The control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common reading unit, which connects to all core rows. The control device is used to control the data channel switching and data reading of the core rows immersed in the reagent through the row switching and common reading unit.

7. The data reading system according to claim 5, characterized in that, The control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common reading unit, which is used to control the time difference between the immersion time of the core row and the data reading time to not exceed a preset range.

8. The data reading system according to claim 5, characterized in that, The control device includes an image processing module connected to the semiconductor sequencing chip. The image processing module includes a row switching and common reading unit. The row switching and common reading unit is used to control the continuous exposure time of the core immersion row through a first timing sequence and to control the movement time of the robot through a second timing sequence. In the first timing sequence, a waiting time is separated from each movement time of the robot in the second timing sequence before and after the continuous exposure time of the core immersion row.

9. The data reading system according to claim 8, characterized in that, The line switching and common reading unit is also used to control the data transmission of the core line through a third timing sequence, wherein the data transmission time of each core line in the third timing sequence is after the continuous exposure time of the corresponding core line.

10. A gene sequencing system, characterized in that, Includes the data reading system according to any one of claims 5-9.

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