A medicament for preventing and treating termites and application thereof

Abstract

The present application belongs to the technical field of green pest control, and discloses a medicament for controlling termites and application thereof. The medicament comprises fipronil and ethyl acetylacetate. The use concentration of the ethyl acetylacetate is 250 μg / g, and the use concentration of the fipronil is 1 μg / g. The ethyl acetylacetate and the fipronil are mixed for use, which can promote the tunneling behavior of Coptotermes formosanus and induce the termites to enter the area treated by the fipronil, so that more termites contact the fipronil and more termites are killed. The ethyl acetylacetate with the use concentration of 250 μg / g enhances the lethal effect of 1 μg / g of the fipronil on the Coptotermes formosanus. The present application reduces the dosage of the fipronil, is green and environmentally friendly, and reduces the pollution to the environment and the influence on other organisms through the synergistic effect of the ethyl acetylacetate.

Description

Technical Field

[0001] This invention belongs to the field of green pest control technology, and more specifically relates to an agent for controlling termites and its application. Background Technology

[0002] The Formosan subterranean termite (Coptotermesformosanus Shiraki) is a widely distributed social pest. In my country, it severely damages buildings, agricultural and forestry production, power and telecommunications systems, and water conservancy projects, causing enormous economic losses. Currently, termite control still relies primarily on chemical methods. Traditional termite control agents include methyl parathion and arsenic trioxide, but due to their severe environmental pollution, they have been replaced by non-repellent, slow-acting termite killers.

[0003] Fipronil is a phenylpyrazole insecticide that primarily acts through stomach poison and contact, exhibiting high efficacy and low toxicity, and is widely used for termite control. When used to kill termites, the concentration of fipronil is 10-100 μg / g. However, long-term, high-dose use of fipronil may pollute the environment, particularly harming aquatic life and bees.

[0004] Therefore, how to provide an agent that reduces the dosage of fipronil, is environmentally friendly, and reduces pollution and impact on other organisms is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] To overcome the above problems, the present invention provides a termite control agent and its application. The mixture of ethyl acetylacetonate and fipronil can promote the tunneling behavior of Formosan subterranean termites. Ethyl acetylacetonate at a concentration of 250 μg / g enhances the lethal effect of 1 μg / g fipronil on Formosan subterranean termites.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An agent for controlling termites, the agent comprising: fipronil and ethyl acetylacetonate.

[0008] Preferably, the concentration of ethyl acetylacetonate used is 250 μg / g, and the concentration of fipronil used is 1 μg / g.

[0009] The present invention also provides the application of the above-mentioned agent in the control of Formosan subterranean termites.

[0010] In this invention, the ethyl acetylacetonate, commonly known as Ethyl 2,4-dioxovalerate, has the following chemical structural formula:

[0011]

[0012] In this invention, the termite is *Dalbergia formosanus*, belonging to the family Rhizotermidae in the order Isoptera.

[0013] As can be seen from the above technical solution, compared with the prior art, the present invention provides a termite control agent with the following beneficial effects:

[0014] (1) This invention uses a mixture of ethyl acetylacetonate and fipronil, which can promote the tunneling behavior of Formosan subterranean termites and induce termites to enter areas treated with fipronil, thereby allowing more termites to come into contact with fipronil and killing more termites. Ethyl acetylacetonate at a concentration of 250 μg / g enhances the lethal effect of 1 μg / g fipronil on Formosan subterranean termites.

[0015] (2) This invention significantly reduces the amount of fipronil used by ethyl acetylacetonate and fipronil through the synergistic effect of the two, making it environmentally friendly, reducing pollution to the environment and impact on other organisms. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 The lethality of each group of pesticides on Formosan subterranean termites is shown. The pesticides were: T1 - chemical-free sand; T2 - sand treated with ethyl acetylacetonate (250 μg / g); T3 - sand treated with fipronil (1 μg / g); T4 - sand treated with ethyl acetylacetonate (250 μg / g) and fipronil (1 μg / g); T5 - sand treated with fipronil (10 μg / g); and T6 - sand treated with ethyl acetylacetonate (250 μg / g) and fipronil (10 μg / g). Detailed Implementation

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1

[0020] An agent for controlling termites, the agent comprising: fipronil and ethyl acetylacetonate.

[0021] Preferably, the concentration of ethyl acetylacetonate used is 250 μg / g, and the concentration of fipronil used is 1 μg / g.

[0022] The above-mentioned agents are used in the control of Formosan subterranean termites.

[0023] The termites in question are *Dalbergia formosanus*, belonging to the family Rhinocerotidae in the order Isoptera.

[0024] Termite experiment

[0025] 1) The termites used in the test were collected from the Arboretum of South China Agricultural University in Guangzhou, representing three different wild populations of Formosan subterranean termites. The collected termites were then transferred to the laboratory and preserved in a plastic container (55×40×31cm).

[0026] 2) The sand used in the experiment was sieved through a 1mm sieve, sterilized at 80℃ for 3 days, and dried at 50℃ for 2 weeks.

[0027] 3) Dissolve ethyl acetylacetate in acetone solution to prepare an acetone solution with ethyl acetylacetate concentration of 5000 μg / mL.

[0028] 4) Using a graduated cylinder, take 30 mL of the acetone solution prepared in step 3) and add it to 600 g of dry sand. Wash the graduated cylinder twice with 15 mL of chemical-free acetone and place it in a resealable bag. Add 60 mL of acetone to the same 600 g of dry sand as a control. Place the prepared sand in a fume hood overnight. After the acetone has completely evaporated, sand containing 250 μg / g ethyl acetylacetate is obtained. Sand containing fipronil alone (1 μg / g or 10 μg / g), and sand containing both ethyl acetylacetate (250 μg / g) and fipronil (1 μg / g or 10 μg / g) were prepared using the same method. Distilled water was added before the experiment to obtain sand with a water content of 15%.

[0029] 5) Conduct bioassays in a flatbed excavation device. Fill one half of the plate with 28g of untreated sand, and fill the other half with 28g of sand treated with ethyl acetylacetonate (250μg / g) or fipronil (1 or 10μg / g) or a mixture of ethyl acetylacetonate (250μg / g) and fipronil (1 or 10μg / g). Place 50 Formosan subterranean termites (47 worker termites and 3 soldier termites) in a circular petri dish (connected to the flatbed excavation device) located in the center of the device.

[0030] 6) As shown in Table 1, there were a total of 6 selection experiments for digging ant tunnels, with each experiment repeated 18 times (3 Formosan subterranean termite colonies × 6 replicates). The plates were placed in a completely dark environment at 24±1℃.

[0031] 7) At 24h and 48h, measure the length and area of ​​termite tunnels dug by Formosan subterranean termites in treated and untreated sand. After 48h, open the plate and count the number of surviving termites.

[0032] Table 1 Grouping information for the ant tunnel selection experiment

[0033]

[0034] Table 2. Activity statistics of termites in each group at 24h and 48h.

[0035]

[0036]

[0037] Table 2 shows that sand treated with ethyl acetylacetonate (250 μg / g) had significantly longer and larger tunnels at 24 and 48 h than untreated sand. There was no significant difference in tunnel length and area between fipronil-treated and untreated sand at 24 and 48 h. However, sand treated with both ethyl acetylacetonate (250 μg / g) and fipronil (1 μg / g) had significantly longer and larger tunnels at 24 and 48 h than untreated sand. This indicates that ethyl acetylacetonate (250 μg / g) alone, or in combination with fipronil (1 μg / g), can promote tunneling behavior in Formosan subterranean termites.

[0038] Figure 1 The results showed no statistically significant difference in termite survival rates between sand treated with ethyl acetylacetonate (250 μg / g) and untreated sand. However, the termite survival rate in sand treated with both ethyl acetylacetonate (250 μg / g) and fipronil (1 μg / g) was significantly lower than that in sand treated with fipronil alone (1 μg / g). This indicates that the combined use of ethyl acetylacetonate (250 μg / g) and fipronil (1 μg / g) has a synergistic effect on the lethality of Formosan subterranean termites.

[0039] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. The solutions disclosed in the embodiments are described simply because they correspond to the methods disclosed in the embodiments; relevant parts can be found in the method section.

[0040] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A termite control agent, characterized in that, The agent comprises: fipronil and ethyl acetylacetonate; the concentration of ethyl acetylacetonate used is 250 μg / g, and the concentration of fipronil used is 1 μg / g.

2. The application of the agent according to claim 1 in the control of Formosan subterranean termites.

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