A specimen collection device for hematopoietic stem cell transplantation and a collection method

Abstract

This invention discloses a specimen collection device and method for hematopoietic stem cell transplantation, including a specimen collection component comprising a collection tube and a top cover. The upper end of the collection tube has a blood collection tube placement cavity and a collection tube placement cavity. The beneficial effects of this invention are: by setting up a negative pressure blood collection tube and a specimen collection tube, blood is collected first, then centrifuged, and finally hematopoietic stem cell specimens are collected, completing the stem cell specimen collection in one step, greatly improving collection efficiency. A double-pass needle is inserted into the negative pressure blood collection tube, with an aspiration channel located at the bottom of the tube and a ventilation channel located at the upper end. The double-pass needle is connected to a ventilation tube, and an air bag is connected to the ventilation tube through a ventilator, increasing the air pressure inside the negative pressure blood collection tube. This allows the hematopoietic stem cells, separated by centrifugation and located in the lower layer, to enter the aspiration channel. The hematopoietic stem cells are then transported to the specimen collection tube via a sampling catheter, achieving stable collection.

Description

Technical Field

[0001] This invention relates to a specimen collection device, specifically a specimen collection device and method for hematopoietic stem cell transplantation, belonging to the field of hematopoietic stem cell transplantation technology. Background Technology

[0002] Hematopoietic stem cells differ from most cells in the body, possessing a strong proliferative and differentiation potential. They can produce large numbers of different types of blood cells as needed, including oxygen-carrying red blood cells, infection-fighting white blood cells, and platelets with clotting functions. Most hematopoietic stem cells are located in the bone marrow, a spongy tissue contained within the hollow spaces of bones. Hematopoietic stem cells outside the bone marrow exist in the blood circulating throughout the body, called peripheral blood stem cells. Both types of stem cells can be used in clinical transplantation to help patients rebuild their hematopoietic function; however, the number of hematopoietic stem cells in these tissues is not substantial. Furthermore, insufficient numbers of immunocompatible donors, graft-versus-host disease, and delayed reconstitution after transplantation are also major factors limiting their clinical application. In vitro culture of hematopoietic stem cells is considered a potentially feasible research direction for increasing the number of transplantable stem cells. Therefore, before performing hematopoietic stem cell transplantation, it is necessary to first sample and culture stem cells from a donor to obtain a sufficient number for transplantation.

[0003] In existing technologies, such as the plasma sample collection device and its operation method for hematopoietic stem cell transplantation disclosed in CN116612904A, during sample collection, an electromagnetic component in conjunction with a spring allows staff to easily remove a cotton swab containing disinfectant from a fixed block to disinfect the area on the patient's arm where blood will be drawn. This avoids the cumbersome operation of manually picking up cotton swabs and wetting them with disinfectant during traditional disinfection, greatly improving blood collection efficiency. However, in the actual sampling process, the collected plasma is not hematopoietic stem cells. Further centrifugation is required to obtain the desired hematopoietic stem cells. Existing technologies only involve plasma collection; subsequent centrifugation and other processes require separate operations, resulting in the inability to complete the sample collection process in one go. Furthermore, the sampling equipment used is bulky and inconvenient to carry. Summary of the Invention

[0004] The purpose of this invention is to provide a specimen collection device and method for hematopoietic stem cell transplantation in order to solve the problems of low specimen collection efficiency and large size that makes it inconvenient to carry.

[0005] The present invention achieves the above objectives through the following technical solution: a specimen collection device for hematopoietic stem cell transplantation, comprising a specimen collection component, the specimen collection component comprising a collection tube and a top cover, the top cover being movably placed on the collection tube, the collection tube being filled with a filling colloid, and the upper end of the collection tube being provided with a blood collection tube placement cavity and a collection tube placement cavity; A negative pressure blood collection tube is placed inside the blood collection tube placement chamber. A rotating drum is rotatably connected to the blood collection tube placement chamber, and a negative pressure blood collection tube is placed inside the rotating drum. A blood collection needle is inserted into the negative pressure blood collection tube, and the needle of the blood collection needle is inserted into the blood collection site of the human body. A specimen collection tube is placed inside the collection tube placement chamber, and a sampling catheter is inserted into the specimen collection tube. The other end of the sampling catheter is connected to a double-port needle. After the blood in the negative pressure blood collection tube is centrifuged, the double-port needle is inserted into the negative pressure blood collection tube. An airbag is fixedly installed inside the top cover, and a ventilation tube is connected to the double-through needle tube. The airbag is connected to the ventilation tube through the air guide tube.

[0006] As a further embodiment of the present invention: a docking stepped surface is provided at the outer edge of the upper end of the collection tube, and the upper cover is placed on the docking stepped surface. A stable base is connected to the bottom end of the collection tube. The stable base is a support base made of silicone material, and a slot is provided on the stable base.

[0007] As a further embodiment of the present invention: a blood collection tube fixing sleeve is fixedly connected to the inner wall of the rotating cylinder, and the blood collection tube fixing sleeve is located in the lower half of the inner cavity of the rotating cylinder. A collection tube fixing sleeve is fixedly connected to the inner wall of the collection tube placement cavity. Linear notches are opened on the inner surfaces of both the blood collection tube fixing sleeve and the collection tube fixing sleeve.

[0008] As a further aspect of the present invention: the upper end of the collection tube is provided with several ice pack placement cavities, which are evenly distributed around the outside of the collection tube placement cavity, and medical strip ice packs are inserted into the ice pack placement cavities.

[0009] As a further embodiment of the present invention: the upper end of the collection tube is also provided with a waste chamber and a conduit storage chamber, and the opening ends of the waste chamber and the conduit storage chamber are connected with a cross-shaped gasket.

[0010] As a further embodiment of the present invention: a battery and a drive motor are embedded in the filling colloid inside the collection tube. A gear cavity is formed in the filling colloid, and a driving gear and a driven gear are installed in the gear cavity. The driving gear is coaxially connected to the rotating shaft of the drive motor, and the driven gear is coaxially connected to the rotating rod at the bottom of the rotating tube. A bearing is sleeved on the rotating rod at the bottom of the rotating tube, and the bearing is embedded in the filling colloid. The battery supplies power to the drive motor. A charging connector is formed at the bottom of the side wall of the collection tube. A control switch is installed in the middle of the side wall of the collection tube. The charging connector is electrically connected to the battery, and the control switch is electrically connected to the connection line between the battery and the drive motor.

[0011] As a further embodiment of the present invention: a conical filter screen is attached to the inner wall of the specimen collection tube, and a cap is attached to the upper opening of the specimen collection tube, with the liquid outlet of the sampling tube inserted at the center of the cap.

[0012] As a further embodiment of the present invention: a sealing plug is provided at the connection between the double-port needle tube and the sampling catheter and the ventilation tube, and the sealing plug is placed at the upper perforation position of the negative pressure blood collection tube. The double-port needle tube includes two cavities: a ventilation cavity and a suction cavity. The ventilation cavity is connected to the ventilation tube, and the suction cavity is connected to the sampling catheter. When the double-port needle tube is inserted into the negative pressure blood collection tube, the suction cavity is located at the bottom of the tube, and the ventilation cavity is located at the upper end of the tube.

[0013] As a further embodiment of the present invention: a soft rubber sleeve is provided at the upper end of the cover, the airbag is tightly attached to the rubber sleeve, the bottom end of the airbag is placed on the fixing block, and the fixing block is fixedly connected to the inner wall of the cover.

[0014] A method for collecting specimens for hematopoietic stem cell transplantation, the method comprising the following steps: Step 1: First, place a negative pressure blood collection tube in the blood collection tube placement chamber of the collection tube, place a specimen collection tube in the collection tube placement chamber, place the medical equipment required for collection in the catheter storage chamber, and place the top cover on the collection tube to facilitate carrying the collection device by hand. Step 2: When collecting blood, open the top cover. The collection tube can be placed vertically on the support surface through the stable base at the bottom. When the negative pressure blood collection tube is placed in the rotating tube, the bottom of the negative pressure blood collection tube can be supported by the blood collection tube fixing sleeve. A blood collection needle is inserted into the negative pressure blood collection tube, and the needle of the blood collection needle is pierced into the blood collection site of the human body to achieve blood collection. Step 3: After blood collection, remove the blood collection needle and place it in the waste chamber. Press down hard on the negative pressure blood collection tube to deform the fixing sleeve of the blood collection tube, so that the entire negative pressure blood collection tube is inserted into the rotating drum. The drive motor drives the meshing transmission of the active gear and the driven gear, which in turn drives the rotating drum to centrifuge the blood in the negative pressure blood collection tube, and separate the red blood cells and white blood cells and other hematopoietic stem cells in the blood. Step 4: Press the specimen collection tube down firmly to insert it into the collection tube placement cavity. Insert the sampling catheter into the specimen collection tube. Insert the double-pass needle into the negative pressure blood collection tube, ensuring that the aspiration channel of the double-pass needle is located at the bottom of the negative pressure blood collection tube and the ventilation channel is located at the top of the negative pressure blood collection tube. Step 5: Connect the air tube to the ventilation tube, and place the top cover on the collection tube again. Press down on the rubber sleeve to force the gas in the airbag into the negative pressure collection tube, thereby increasing the air pressure in the negative pressure collection tube. Step Six: The pressure inside the negative pressure blood collection tube increases, allowing the hematopoietic stem cells separated by centrifugation and located in the lower layer of the tube to enter the aspiration cavity. The hematopoietic stem cells can then be transported to the specimen collection tube via the sampling catheter. The collected specimen is filtered through a conical filter before entering the collection tube, which can filter and separate residual tissue fragments in the specimen to ensure the qualification rate of the collected specimen.

[0015] The beneficial effects of this invention are: Equipped with negative pressure blood collection tubes and specimen collection tubes, blood can be collected first, and then the blood can be centrifuged before hematopoietic stem cell specimens can be collected. This allows for the complete collection of stem cell specimens in one go, greatly improving collection efficiency. Furthermore, both the negative pressure blood collection tubes and specimen collection tubes are located inside the collection cylinder. Placing the top cover connector on the top of the collection cylinder provides some protection for the collection device when not in use, and it is also easy to carry and operate. The system is equipped with a double-port needle inserted into a negative pressure blood collection tube. The aspiration chamber is located at the bottom of the tube, and the ventilation chamber is located at the top of the tube. The double-port needle is connected to a ventilation tube, and the air bag is connected to the ventilation tube through a ventilator. By pressing down on the rubber sleeve, the gas inside the air bag is forced into the negative pressure blood collection tube, thereby increasing the air pressure inside the tube. This allows the hematopoietic stem cells, which have been separated by centrifugation and are located in the lower layer, to enter the aspiration chamber. The hematopoietic stem cells can then be transported to the specimen collection tube via a sampling catheter. The separation of stem cells is achieved by utilizing the principle of air pressure, which can achieve stable collection. By inserting a conical filter screen into the inner wall of the specimen collection tube, the collected specimens can be filtered through the conical filter screen before entering the collection tube during hematopoietic stem cell specimen collection. This allows residual tissue fragments in the specimens to be filtered and separated, ensuring the quality of the collected specimens. Several ice pack placement chambers are provided at the upper end of the collection tube, which can cool the collection tube placement chamber, keeping the specimen collection tube at a low temperature and enabling cryopreservation of the collected specimen. The upper end of the collection tube also has a waste chamber and a catheter storage chamber. The opening ends of the waste chamber and the catheter storage chamber are connected to cross-shaped gaskets, which can be used to place medical supplies used in the specimen collection process and waste after use, and the gaskets can prevent items from falling. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the blood collection tube structure during the blood collection process of this invention; Figure 3 This is a schematic diagram of the sampling cylinder structure during the sampling state of the present invention; Figure 4 This is a schematic diagram of the cross-sectional structure of the upper cover of the present invention; Figure 5 This is a schematic diagram of the cross-sectional structure of the collection cylinder of the present invention; Figure 6 This is a schematic diagram of the cross-sectional structure of the blood collection tube placement cavity and the collection tube placement cavity of the present invention; Figure 7 This is a partially enlarged structural diagram of the blood collection tube placement cavity of the present invention; Figure 8 This is a schematic diagram of the drive motor drive state structure of the present invention; Figure 9 This is a schematic diagram of the planar structure of the dual-channel needle tube of the present invention; Figure 10 This is a schematic diagram of the cross-sectional structure of the double-through needle tube of the present invention; Figure 11 This is a schematic diagram of the specimen collection tube structure of the present invention.

[0017] In the diagram: 1. Collection tube; 11. Docking stepped surface; 12. Stabilizing base; 13. Blood collection tube placement chamber; 131. Rotating drum; 132. Blood collection tube fixing sleeve; 14. Collection tube placement chamber; 141. Collection tube fixing sleeve; 15. Ice pack placement chamber; 16. Waste chamber; 17. Catheter storage chamber; 18. Filling colloid; 19. Battery; 110. Drive motor; 111. Drive gear; 112. Driven gear; 113. Gear chamber. 114. Charging connector; 115. Control switch; 116. Bearing; 2. Top cover; 21. Rubber sleeve; 22. Fixing block; 23. Airbag; 24. Air tube; 3. Negative pressure blood collection tube; 31. Blood collection needle tube; 4. Specimen collection tube; 41. Conical filter screen; 42. Cap; 5. Sampling catheter; 51. Ventilation tube; 52. Sealing plug; 53. Double-pass needle tube; 54. Ventilation cavity; 55. Suction cavity; 6. Medical long strip ice pack. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1

[0020] like Figure 1 , Figure 5 , Figure 6 and Figure 9As shown, a specimen collection device for hematopoietic stem cell transplantation includes a specimen collection component, which includes a collection tube 1 and a top cover 2. The top cover 2 is movably placed on the collection tube 1. The collection tube 1 is filled with a filling colloid 18. The upper end of the collection tube 1 has a blood collection tube placement cavity 13 and a collection tube placement cavity 14. A negative pressure blood collection tube 3 is placed in the blood collection tube placement chamber 13. A rotating drum 131 is rotatably connected to the blood collection tube placement chamber 13, and a negative pressure blood collection tube 3 is placed in the rotating drum 131. A blood collection needle 31 is inserted into the negative pressure blood collection tube 3, and the needle of the blood collection needle 31 is pierced at the blood collection site of the human body. A specimen collection tube 4 is placed in the collection tube placement chamber 14. A sampling catheter 5 is inserted into the specimen collection tube 4. The other end of the sampling catheter 5 is connected to a double-pass needle 53. After the blood in the negative pressure blood collection tube 3 is centrifuged, the double-pass needle 53 is inserted into the negative pressure blood collection tube 3. An airbag 23 is fixedly installed inside the upper cover 2. A double-through needle tube 53 is connected to a ventilation tube 51. The airbag 23 is connected to the ventilation tube 51 through a ventilator tube 24.

[0021] Example 2

[0022] like Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 and Figure 7 As shown, in addition to all the technical features included in Embodiment 1, this embodiment also includes: A docking stepped surface 11 is provided on the outer edge of the upper end of the collection tube 1, and the upper cover 2 is placed on the docking stepped surface 11. The bottom end of the collection tube 1 is connected to a stable base 12. The stable base 12 is a support base made of silicone material, and the stable base 12 has a slot for mounting the upper cover 2 on the upper end of the collection tube 1. When not in use, it can provide a certain degree of protection for the collection device. When in use, the collection tube 1 can be placed on the stable base 12 so that the collection device can be placed vertically on the support surface to prevent it from tipping over.

[0023] A blood collection tube fixing sleeve 132 is fixedly connected to the inner wall of the rotating drum 131, and the blood collection tube fixing sleeve 132 is located in the lower half of the inner cavity of the rotating drum 131. A collection tube fixing sleeve 141 is fixedly connected to the inner wall of the collection tube placement cavity 14. Both the blood collection tube fixing sleeve 132 and the collection tube fixing sleeve 141 have linear notches on their inner surfaces, so that when the negative pressure blood collection tube 3 is placed in the rotating drum 131, the bottom of the negative pressure blood collection tube 3 can be supported by the blood collection tube fixing sleeve 132, that is, the upper half of the negative pressure blood collection tube 3 is located in the rotating drum 131. On the outside, the blood volume can be directly observed, allowing for timely termination of the blood collection process. By pressing down on the negative pressure blood collection tube 3, the fixing sleeve 132 of the blood collection tube can be deformed, thus allowing the entire negative pressure blood collection tube 3 to be inserted into the rotating drum 131 and fixed within it. Similarly, when the specimen collection tube 4 is pressed down to be inserted into the collection tube placement cavity 14, the fixing sleeve 141 of the collection tube deforms, ensuring that the specimen collection tube 4 is fixed in place and remains stable during specimen collection.

[0024] Example 3

[0025] like Figure 1 , Figure 3 , Figure 5 and Figure 8 As shown, in addition to all the technical features included in Embodiment 1, this embodiment also includes: The upper end of the collection tube 1 is also provided with several ice pack placement chambers 15. The ice pack placement chambers 15 are evenly distributed around the outside of the collection tube placement chamber 14. Medical long strip ice packs 6 are inserted in the ice pack placement chambers 15, which can cool the collection tube placement chamber 14, that is, keep the specimen collection tube 4 at a low temperature, and form a low temperature preservation of the collected specimen.

[0026] The upper end of the collection tube 1 is also provided with a waste chamber 16 and a catheter storage chamber 17. The opening ends of the waste chamber 16 and the catheter storage chamber 17 are connected with cross-shaped gaskets so that medical supplies used in the specimen collection process and waste after use can be placed separately, and the gaskets can prevent items from falling.

[0027] A battery 19 and a drive motor 110 are embedded in the filling colloid 18 inside the collection tube 1. A gear cavity 113 is formed inside the filling colloid 18, and a driving gear 111 and a driven gear 112 are installed in the gear cavity 113. The driving gear 111 is coaxially connected to the rotating shaft of the drive motor 110, and the driven gear 112 is coaxially connected to the rotating rod at the bottom end of the rotating drum 131. A bearing 116 is fitted on the rotating rod at the bottom end of the rotating drum 131 and is embedded in the filling colloid 18. The battery 19 supplies power to the drive motor 110. The bottom of the side wall of the collection tube 1... A charging connector 114 is provided, and a control switch 115 is installed in the middle of the side wall of the collection tube 1. The charging connector 114 is electrically connected to the storage battery 19, and the control switch 115 is electrically connected to the connection line between the storage battery 19 and the drive motor 110. The drive motor 110 drives the meshing transmission of the active gear 111 and the driven gear 112, thereby driving the rotating drum 131 to rotate. This allows the blood in the negative pressure collection tube 3 to be centrifuged, separating the hematopoietic stem cells such as red blood cells and white blood cells in the blood to the lower layer, so as to facilitate the collection of hematopoietic stem cell samples.

[0028] Example 4

[0029] like Figure 1 , Figure 4 , Figure 9 , Figure 10 and Figure 11 As shown, in addition to all the technical features included in Embodiment 1, this embodiment also includes: A conical filter 41 is attached to the inner wall of the specimen collection tube 4, and a cap 42 is attached to the upper opening of the specimen collection tube 4. The liquid outlet of the sampling catheter 5 is inserted into the center of the cap 42. When collecting hematopoietic stem cell specimens, the collected specimens are filtered through the conical filter 41 before entering the collection tube, so as to filter and separate residual tissue fragments in the specimens and ensure the qualification rate of the collected specimens.

[0030] A sealing plug 52 is fitted at the connection between the dual-port needle tube 53 and the sampling catheter 5 and the ventilation tube 51, and the sealing plug 52 is placed at the upper perforation position of the negative pressure blood collection tube 3. The dual-port needle tube 53 includes two cavities: a ventilation cavity 54 and an aspiration cavity 55. The ventilation cavity 54 is connected to the ventilation tube 51, and the aspiration cavity 55 is connected to the sampling catheter 5. When the dual-port needle tube 53 is inserted into the negative pressure blood collection tube 3, the aspiration cavity 55 is located at the bottom of the tube, and the ventilation cavity 54 is located at the upper end of the tube. Air can be vented into the negative pressure blood collection tube 3 through the ventilation cavity 54, thereby increasing the pressure inside the negative pressure blood collection tube 3, so that the hematopoietic stem cells separated by centrifugation and located in the lower layer of the tube can enter the aspiration cavity 55, and then the hematopoietic stem cells can be transported to the specimen collection tube 4 through the sampling catheter 5.

[0031] The upper end of the cover 2 is provided with a soft rubber sleeve 21, and the airbag 23 is tightly attached to the rubber sleeve 21. The bottom end of the airbag 23 is placed on the fixing block 22, and the fixing block 22 is fixedly connected to the inner wall of the cover 2, so that by pressing down the rubber sleeve 21, the gas in the airbag 23 can be forced into the negative pressure blood collection tube 3, thereby increasing the air pressure in the negative pressure blood collection tube 3.

[0032] Example 5

[0033] A method for collecting specimens for hematopoietic stem cell transplantation, the method comprising the following steps: Step 1: First, place the negative pressure blood collection tube 3 in the blood collection tube placement cavity 13 of the collection tube 1, place the specimen collection tube 4 in the collection tube placement cavity 14, place the medical equipment required for collection in the catheter storage cavity 17, and place the top cover 2 on the collection tube 1 so that the collection device can be carried by hand. Step 2: When collecting blood, open the top cover 2. The collection tube 1 can be placed vertically on the support surface through the stable base 12 at the bottom. When the negative pressure blood collection tube 3 is placed in the rotating tube 131, the bottom of the negative pressure blood collection tube 3 can be supported by the blood collection tube fixing sleeve 132. A blood collection needle 31 is inserted into the negative pressure blood collection tube 3, and the needle of the blood collection needle 31 is pierced at the blood collection site of the human body to achieve blood collection. Step 3: After blood collection, remove the blood collection needle 31 and place it in the waste chamber 16. Press down hard on the negative pressure blood collection tube 3 to deform the blood collection tube fixing sleeve 132, so that the negative pressure blood collection tube 3 is completely inserted into the rotating drum 131. The drive motor 110 drives the meshing transmission of the drive gear 111 and the driven gear 112, thereby driving the rotating drum 131 to rotate, so as to centrifuge the blood in the negative pressure blood collection tube 3 and separate the hematopoietic stem cells such as red blood cells and white blood cells in the blood. Step 4: Press down the specimen collection tube 4 firmly to insert it into the collection tube placement cavity 14. Insert the sampling catheter 5 into the specimen collection tube 4. Insert the double-pass needle tube 53 into the negative pressure blood collection tube 3, and make the aspiration channel 55 of the double-pass needle tube 53 located at the bottom end of the negative pressure blood collection tube 3, and the ventilation channel 54 located at the upper end of the negative pressure blood collection tube 3. Step 5: Connect the air guide tube 24 to the air inlet tube 51, and place the top cover 2 on the collection tube 1 again. By pressing down on the rubber sleeve 21, the gas in the air bag 23 is forced into the negative pressure collection tube 3 to increase the air pressure in the negative pressure collection tube 3. Step Six: The pressure inside the negative pressure blood collection tube 3 is increased to allow the hematopoietic stem cells separated by centrifugation and located in the lower layer of the tube to enter the aspiration cavity 55. The hematopoietic stem cells can then be transported to the specimen collection tube 4 via the sampling catheter 5. The collected specimen is filtered through the conical filter 41 before entering the collection tube to filter and separate residual tissue fragments in the specimen, thereby ensuring the qualification rate of the collected specimen.

[0034] Working principle: The drive motor 110 drives the meshing transmission of the active gear 111 and the driven gear 112, which in turn drives the rotating drum 131 to rotate, so as to centrifuge the blood in the negative pressure blood collection tube 3, and separate the hematopoietic stem cells such as red blood cells and white blood cells in the blood. By pressing down the rubber sleeve 21, the gas in the air bag 23 is forced into the negative pressure blood collection tube 3, so as to increase the air pressure in the negative pressure blood collection tube 3, so that the hematopoietic stem cells separated by centrifugation and located in the lower layer of the tube can enter the suction cavity 55, and then the hematopoietic stem cells can be transported to the specimen collection tube 4 through the sampling catheter 5.

[0035] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0036] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A specimen collection device for hematopoietic stem cell transplantation comprising a specimen collection assembly, characterized by: The specimen collection assembly includes a collection tube (1) and a top cover (2). The top cover (2) is movably placed on the collection tube (1). The collection tube (1) is filled with a filling colloid (18). The upper end of the collection tube (1) is provided with a blood collection tube placement cavity (13) and a collection tube placement cavity (14). A negative pressure blood collection tube (3) is placed in the blood collection tube placement cavity (13). A rotating cylinder (131) is rotatably connected to the blood collection tube placement cavity (13), and a negative pressure blood collection tube (3) is placed in the rotating cylinder (131). A blood collection needle (31) is inserted into the negative pressure blood collection tube (3), and the needle of the blood collection needle (31) is pierced at the blood collection site of the human body. A specimen collection tube (4) is placed in the collection tube placement cavity (14), and a sampling catheter (5) is inserted into the specimen collection tube (4). The other end of the sampling catheter (5) is connected to a double-pass needle (53). After the blood in the negative pressure blood collection tube (3) is centrifuged, the double-pass needle (53) is inserted into the negative pressure blood collection tube (3). The dual-port needle tube (53) includes two cavities: a ventilation cavity (54) and a suction cavity (55). The ventilation cavity (54) is connected to the ventilation tube (51), and the suction cavity (55) is connected to the sampling catheter (5). When the dual-port needle tube (53) is inserted into the negative pressure blood collection tube (3), the suction cavity (55) is located at the bottom of the tube, and the ventilation cavity (54) is located at the top of the tube. An airbag (23) is fixedly installed inside the upper cover (2), and the double-through needle tube (53) is connected to a ventilation tube (51). The airbag (23) is connected to the ventilation tube (51) through a duct tube (24).

2. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The upper outer edge of the collection tube (1) is provided with a docking stepped surface (11), and the upper cover (2) is placed on the docking stepped surface (11). The bottom end of the collection tube (1) is connected to a stable base (12). The stable base (12) is a support base made of silicone material, and the stable base (12) is provided with a mounting slot.

3. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The inner wall of the rotating cylinder (131) is fixedly connected to a blood collection tube fixing sleeve (132), and the blood collection tube fixing sleeve (132) is located in the lower half of the inner cavity of the rotating cylinder (131). The inner wall of the collection tube placement cavity (14) is fixedly connected to a collection tube fixing sleeve (141). The inner surfaces of the blood collection tube fixing sleeve (132) and the collection tube fixing sleeve (141) are both provided with linear notches.

4. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The upper end of the collection tube (1) is also provided with several ice pack placement chambers (15). The ice pack placement chambers (15) are evenly distributed around the outside of the collection tube placement chamber (14). Medical long strip ice packs (6) are inserted in the ice pack placement chambers (15).

5. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The upper end of the collection tube (1) is also provided with a waste chamber (16) and a conduit storage chamber (17), and the opening ends of the waste chamber (16) and the conduit storage chamber (17) are connected with a cross-shaped gasket.

6. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The collection tube (1) contains a battery (19) and a drive motor (110) embedded in a filling colloid (18). A gear cavity (113) is formed inside the filling colloid (18). A driving gear (111) and a driven gear (112) are meshed and connected inside the gear cavity (113). The driving gear (111) is coaxially connected to the rotating shaft of the drive motor (110). The driven gear (112) is coaxially connected to the rotating rod at the bottom of the rotating drum (131). The rotation of the bottom of the rotating drum (131) A bearing (116) is fitted on the rod body. The bearing (116) is embedded in the filling colloid (18). The storage battery (19) supplies power to the drive motor (110). A charging connector (114) is provided at the bottom of the side wall of the collection tube (1). A control switch (115) is installed in the middle of the side wall of the collection tube (1). The charging connector (114) is electrically connected to the storage battery (19). The control switch (115) is electrically connected to the connection line between the storage battery (19) and the drive motor (110).

7. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The inner wall of the specimen collection tube (4) is fitted with a conical filter screen (41), and a cap (42) is fitted at the upper opening of the specimen collection tube (4). The liquid outlet of the sampling conduit (5) is inserted at the center of the cap (42).

8. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: A sealing plug (52) is fitted at the connection between the double-pass needle tube (53) and the sampling catheter (5) and the ventilation tube (51), and the sealing plug (52) is placed at the upper perforation position of the negative pressure blood collection tube (3).

9. The specimen collection device for hematopoietic stem cell transplantation according to claim 1, characterized in that: The upper end of the cover (2) is provided with a soft rubber sleeve (21), the airbag (23) is tightly attached to the rubber sleeve (21), the bottom end of the airbag (23) is placed on the fixing block (22), and the fixing block (22) is fixedly connected to the inner wall of the cover (2).

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