A traditional Chinese medicine composition with anti-wrinkle effects and its preparation method

Abstract

This invention discloses a traditional Chinese medicine composition with anti-wrinkle effects and its preparation method. By weight, the traditional Chinese medicine composition is prepared from the following raw materials: 100-200 parts ginseng, 100-200 parts kudzu root, 50-150 parts Solomon's seal rhizome, 10-100 parts Poria cocos, 10-100 parts Angelica sinensis, 1-5 parts pearl, 0.1-3 parts artificial bezoar, and 0.1-3 parts borneol. This invention specifically selects five medicinal materials—ginseng, kudzu root, Poria cocos, Solomon's seal rhizome, and Angelica sinensis—from the perspective of traditional Chinese medicine's prevention and treatment of wrinkles, emphasizing the benefits of invigorating qi, nourishing yin, and moisturizing dryness. The addition of artificial bezoar and pearl further enhances the product's skin-beautifying and antioxidant effects. The addition of borneol promotes the transdermal absorption of other active ingredients, significantly improving the product's skin-firming and anti-aging effects. This invention, through the selection of superior traditional Chinese medicine ingredients and optimization of the preparation process, results in a traditional Chinese medicine composition with significant anti-wrinkle effects, making it suitable as a cosmetic additive in various cosmetics and convenient to use.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition with anti-wrinkle effects and its preparation method. Background Technology

[0002] Skin wrinkles are lines of wrinkle formation on the skin caused by degenerative changes in the morphology and structure of dermal collagen fibers, elastic fibers, and matrix, as well as by a reduction in subcutaneous fat and loss of skin moisture, resulting from the long-term, repetitive traction of limb joints and local subcutaneous muscles. Traditional Chinese medicine does not have a corresponding disease name for wrinkles; based on their clinical characteristics and manifestations, they can be categorized under terms such as "anti-aging and wrinkle removal," "reducing wrinkles," and "anti-aging and preventing aging."

[0003] Modern medicine believes that wrinkles are mainly the result of natural skin aging and photoaging, and are related to multiple factors. With age, the natural moisturizing factors in the stratum corneum of the epidermis decrease, the skin's hydration capacity declines, leading to reduced moisture in skin cells, cell shrinkage, aging, and the appearance of fine wrinkles. Under the influence of various factors, the number of dermal fibroblasts decreases, the ratio of collagen types reverses, and elastic fibers in the papillary and reticular layers of the dermis decrease and degenerate. Disordered arrangement of collagen and elastic fibers leads to wrinkle formation. With age, the volume of subcutaneous fat cells decreases, and adipose tissue gradually atrophies, causing the fibrous trabeculae in the lower reticular layer of the dermis and fascia to lose support, resulting in skin laxity. Combined with the effects of gravity, this causes the loose skin to sag, forming wrinkles. Furthermore, facial wrinkles are also related to facial expression muscles. These muscles attach to the skin, and when they contract, wrinkles appear at right angles to the contraction, increasing in number and depth with age.

[0004] Based on this, the invention patent with publication number CN112704712A discloses a traditional Chinese medicine composition for eye care, which selects traditional Chinese medicine materials such as Ligusticum chuanxiong, Poria cocos, Atractylodes macrocephala, Bletilla striata, Ampelopsis japonica, artificial bezoar, pearl, and borneol. Through reasonable combination, the resulting additive has good antioxidant, whitening, and blood-activating effects. This traditional Chinese medicine additive has multiple effects, but its anti-wrinkle effect on the eyes is insufficient.

[0005] In view of this, it is proposed that the present invention provides a traditional Chinese medicine composition with anti-wrinkle effect and a method for preparing the same. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to provide a traditional Chinese medicine composition with anti-wrinkle effects and its preparation method. By optimizing the preparation method, the prepared product exhibits good anti-wrinkle effects.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a traditional Chinese medicine composition with anti-wrinkle effects, made from the following raw materials in parts by weight:

[0009]

[0010] In the above technical solution, the herbal composition of the present invention is composed of eight medicinal materials: ginseng, kudzu root, poria cocos, polygonatum odoratum, angelica sinensis, pearl, artificial bezoar, and borneol. This formula emphasizes invigorating qi, nourishing yin, and moistening dryness. Ginseng and kudzu root are the principal herbs, which have the effects of promoting body fluid production and nourishing blood. Poria cocos, angelica sinensis, and polygonatum odoratum are the assistant herbs, which have the effects of dredging qi and blood, nourishing yin and moistening dryness, and promoting body fluid production and quenching thirst. Borneol is the adjuvant herb, which opens the orifices, disperses stagnant heat, reduces swelling and relieves pain. Pearl and artificial bezoar are the guiding herbs, which have the effect of harmonizing the various herbs, in line with the basic principles of traditional Chinese medicine for the prevention and treatment of wrinkles.

[0011] Ginseng, hailed as the "King of Herbs," possesses the effects of regulating qi and blood, calming the mind and improving intelligence, promoting body fluid production and quenching thirst, and nourishing and strengthening the body. Ginseng contains various ginsenosides, amino acids, vitamins, and minerals, which can promote blood circulation in subcutaneous capillaries, strengthen cell vitality, promote collagen activity, and delay skin aging. The phloretin in ginseng root can also effectively improve skin elasticity, reduce deep wrinkles, improve sagging skin, enhance skin contour and firmness, and tighten the skin.

[0012] Kudzu root is rich in flavonoids, which can neutralize free radicals, reduce oxidative damage to the skin, and thus slow down the aging process. It also contains abundant vitamin C and vitamin E, natural antioxidants that promote collagen production, increasing skin elasticity and firmness. Kudzu root has excellent antioxidant properties; these antioxidants help maintain skin elasticity, reduce wrinkles and fine lines, and make the skin look smoother and younger.

[0013] The main components of Poria cocos are polysaccharides, triterpenoids, and flavonoids, which can promote skin cell metabolism, lighten melanin deposition, and improve skin texture. Poria cocos also promotes the proliferation of integrins, which can reflect the proliferation of fibroblasts and the adhesion between cells and fibrin. The proliferation of integrins can reduce the diameter and volume of collagen wrapped by fibroblasts, thereby shrinking pores and reducing wrinkles.

[0014] Polygonatum odoratum has the effects of nourishing yin and moisturizing dryness, promoting body fluid and quenching thirst. It contains Polygonatum odoratum polysaccharide, which is a unique fructan. The rich polysaccharides and water-soluble substances can quickly penetrate into the deep layers of the skin and lock in moisture for a long time, thus effectively improving dryness and roughness. Polygonatum odoratum is rich in a variety of effective ingredients and nutrients (such as polysaccharides, vitamins, amino acids, etc.). These ingredients can remove free radicals, reduce skin cell damage, and promote metabolism, thereby delaying the skin aging process.

[0015] Angelica sinensis has the effects of promoting blood circulation, removing blood stasis, enhancing capillary blood circulation, improving and enhancing skin metabolism. Angelica sinensis also has good SOD-like activity, inhibiting membrane lipid peroxidation and free radical reactions caused by superoxide free radicals, and binding with biological membrane phospholipids to protect membrane lipids. It has multiple mechanisms to antagonize the damage of free radicals to tissues and has a good anti-aging and antioxidant effect.

[0016] Pearls contain more than 20 kinds of trace elements, as well as vitamin B, taurine, small molecule active peptides and other ingredients. Since ancient times, they have been used as a traditional Chinese medicine and regarded as a beauty product. They have the effects of clearing the eyes and skin, relieving acne and treating toxins, and beautifying the skin. Modern research shows that pearl extract has a good effect of scavenging free radicals and has the effects of tightening the skin and anti-aging.

[0017] Artificial bezoar is made from bovine bile powder, cholic acid, porcine deoxycholic acid, taurine, bilirubin, cholesterol, trace elements, etc. Among them, bilirubin is a natural antioxidant that helps the body resist lipid peroxidation and scavenge free radicals. It is one of the main defense mechanisms of the body against free radical oxidative damage and has a good antioxidant effect.

[0018] Borneol has the effects of clearing the orifices, dispersing stagnant heat, reducing swelling and relieving pain. Borneol itself can be absorbed through the skin and can promote the transdermal absorption of other active ingredients. Its transdermal absorption mainly acts on the stratum corneum, which greatly improves the anti-wrinkle effect of the product of this invention.

[0019] As a preferred embodiment of the present invention, the herbal composition is made from the following raw materials in parts by weight:

[0020]

[0021]

[0022] As a more preferred technical solution of the present invention, the herbal composition is made from the following raw materials in parts by weight:

[0023]

[0024] Secondly, the present invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0025] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, put them in a steam explosion machine for steam explosion treatment, and then depressurize to obtain depressurized material;

[0026] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction to obtain filtrate and filter residue;

[0027] (3) Add water to the filter residue obtained in step (2) and treat it under ultrasound, then filter to obtain filter residue liquid;

[0028] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol for alcohol precipitation, let stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to obtain concentrated Chinese herbal medicine.

[0029] (5) Dissolve artificial bezoar and borneol in a solvent, then add pearl and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence, and finally add solvent and deionized water to mix well. After filtration, the product is obtained.

[0030] As a more preferred technical solution of the present invention, in step (1), saturated steam is used for steam explosion and the steam is treated at 1.1-1.2 MPa for 1-3 minutes.

[0031] As a more preferred technical solution of the present invention, in step (2), the countercurrent extraction temperature is 20-25°C, the countercurrent extraction time is 10-30 min, and the countercurrent extraction solvent is an aqueous solution containing 1-1.2 g / L Tween 80.

[0032] As a more preferred technical solution of the present invention, in step (3), the ultrasonic treatment power is 80-120W, the ultrasonic treatment time is 0.5-5h, and the mass ratio of filter residue to water is 1:8-15.

[0033] As a more preferred technical solution of the present invention, in step (4), the ethanol concentration of the alcohol precipitation is 50-70 wt%; and the concentration is carried out under reduced pressure to a relative density of 1.15-1.25.

[0034] As a more preferred technical solution of the present invention, in step (5), the solvent is one or more of butanediol, 1,3-propanediol, or glycerol; filtration is performed sequentially using 1.0 μm and 0.60 μm microporous filter membranes and titanium rods. Preferably, the solvent is 1,3-propanediol, because 1,3-propanediol has good safety and moisturizing properties, and it has excellent solubility for the herbal medicines of the present invention.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) This invention provides a traditional Chinese medicine composition with anti-wrinkle effect, which is prepared by combining eight traditional Chinese medicine materials. Ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis are specifically selected. From the perspective of traditional Chinese medicine prevention and treatment of wrinkles, the composition focuses on tonifying qi, nourishing yin and moisturizing dryness. Artificial bezoar and pearl are added to further enhance the skin beautifying and antioxidant effects of the product. Borneol is added to promote the transdermal absorption of other active ingredients, which greatly improves the skin tightening and anti-aging effects of the product.

[0037] (2) The herbal composition of the present invention has excellent DPPH free radical scavenging, hydroxyl free radical scavenging and superoxide anion scavenging effects, and also has a good inhibitory effect on skin photoaging and significant anti-wrinkle effect.

[0038] (3) Regarding the preparation method of the traditional Chinese medicine composition, this invention abandons the traditional water extraction and alcohol precipitation process and makes targeted optimizations. Specifically: this invention first performs steam explosion treatment on ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis to break cell tissues, which facilitates the subsequent countercurrent immersion to improve the efficiency of extracting the effective components; at the same time, the countercurrent immersion uses an aqueous solvent containing Tween 80 as the extraction solvent, which is safe and non-toxic, and compared with the traditional aqueous solvent, the anti-wrinkle effect of the obtained traditional Chinese medicine composition is more significant, because the addition of Tween 80 helps to reduce interfacial tension and facilitates the extraction and dissolution of components; for the treatment of the filter residue, ultrasonic-assisted water extraction is further adopted to minimize the loss of effective components. Finally, alcohol precipitation is performed to obtain the traditional Chinese medicine extract; in the process of adding artificial bezoar, borneol and pearl, the applicant also optimized the solvent, that is, using 1,3-propanediol, which has better solubility.

[0039] In summary, by selecting superior Chinese herbal medicine ingredients and optimizing the preparation process, the present invention enables the prepared Chinese herbal medicine composition to have significant anti-wrinkle effects, making it suitable for use as a cosmetic additive in various cosmetics and convenient to use. Attached Figure Description

[0040] Figure 1 To investigate the protective concentrations of different concentrations of Babao Anti-wrinkle Formula in a skin photoaging model using the CCK-8 assay. n = 5 / group, **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group.

[0041] Figure 2 The staining results (A) and the positive cell count (B) of different concentrations of Babao Anti-Wrinkle Formula were studied using β-galactosidase staining to investigate the anti-HSF cell senescence effect. n=3 / group, **P<0.01 vs. normal control group; #P<0.05 vs. model group; ##P<0.01 vs. model group.

[0042] Figure 3 To investigate the effects of Babao Anti-Wrinkle Formula on the levels of type I collagen, MMP-1, and MMP-3 in the supernatant of photoaged HSF cells using ELISA. n = 5 / group, *P < 0.05 vs. normal control group; **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group. Detailed Implementation

[0043] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0044] Unless otherwise specified, all medicinal materials used in this invention were purchased through market channels. Similarly, the equipment used in this invention, such as steam explosion and countercurrent extraction equipment, were also purchased commercially. For ease of operation, the pearl in the herbal composition is added in the form of pearl extract.

[0045] Example 1

[0046] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0047]

[0048] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0049] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0050] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution containing 1.1 g / L Tween 80.

[0051] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0052] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0053] (5) Dissolve artificial bezoar and borneol in 0.8L of 1,3-propanediol, then add pearl extract (equivalent to 3g of pearl) and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence. Finally, add 4L of 1,3-propanediol and deionized water and mix well. After filtering with titanium rods and 1.0μm and 0.60μm microporous filter membranes in sequence, the Chinese herbal medicine extract is obtained.

[0054] Example 2

[0055] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0056]

[0057] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0058] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0059] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution containing 1.1 g / L Tween 80.

[0060] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0061] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0062] (5) Dissolve artificial bezoar and borneol in 0.8L of 1,3-propanediol, then add pearl extract (equivalent to 3g of pearl) and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence. Finally, add 4L of 1,3-propanediol and deionized water and mix well. After filtering with titanium rods and 1.0μm and 0.60μm microporous filter membranes in sequence, the Chinese herbal medicine extract is obtained.

[0063] Example 3

[0064] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0065]

[0066] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0067] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0068] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution containing 1.1 g / L Tween 80.

[0069] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0070] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0071] (5) Dissolve artificial bezoar and borneol in 0.8L of 1,3-propanediol, then add pearl extract (equivalent to 3g of pearl) and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence. Finally, add 4L of 1,3-propanediol and deionized water and mix well. After filtering with titanium rods and 1.0μm and 0.60μm microporous filter membranes in sequence, the Chinese herbal medicine extract is obtained.

[0072] Comparative Example 1

[0073] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0074]

[0075] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0076] (1) Take ginseng, angelica, poria, polygonatum and angelica by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0077] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution containing 1.1 g / L Tween 80.

[0078] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0079] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0080] (5) Dissolve artificial bezoar and borneol in 0.8L of 1,3-propanediol, then add the concentrated Chinese herbal medicine solution obtained in step (4), and finally add 4L of 1,3-propanediol and deionized water and mix well. After filtering with 1.0μm and 0.60μm microporous membrane titanium rods, the Chinese herbal medicine extract is obtained.

[0081] Compared with Example 1, in Comparative Example 1, kudzu root was replaced with angelica root, and the use of pearl extract was omitted.

[0082] Comparative Example 2

[0083] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0084]

[0085]

[0086] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0087] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum, angelica sinensis and bletilla striata by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0088] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution containing 1.1 g / L Tween 80.

[0089] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0090] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0091] (5) Dissolve borneol in 0.8L of 1,3-propanediol, then add pearl extract (equivalent to 3g of pearl) and the concentrated Chinese herbal medicine obtained in step (4) in sequence. Finally, add 4L of 1,3-propanediol and deionized water and mix well. Filter the solution through titanium rods with 1.0μm and 0.60μm microporous membranes in sequence to obtain the Chinese herbal medicine extract.

[0092] Compared with Example 1, Comparative Example 2 omitted the use of artificial bezoar and added 50g of Bletilla striata.

[0093] Comparative Example 3

[0094] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0095]

[0096]

[0097] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0098] (1) Take ginseng, kudzu root, Solomon's seal, poria cocos and angelica sinensis, add water and decoct three times, each time for 1.5 hours, add 10 times the amount of water each time, filter the decoction, combine the filtrates, and concentrate under reduced pressure to a relative density of 1.06 (60℃) to obtain a clear herbal extract.

[0099] (2) Add ethanol to make the ethanol concentration reach 70wt%, seal, refrigerate and stand for 24 hours, filter, and recover ethanol from the filtrate under reduced pressure until there is no alcohol taste. Continue to concentrate the medicinal solution to a relative density of 1.2 (60℃) to obtain a concentrated Chinese herbal medicine solution.

[0100] (3) Take artificial bezoar and borneol, add 0.8L of 1,3-propanediol and heat until dissolved, add pearl extract (equivalent to 3g of pearl), then add 4L of 1,3-propanediol and deionized water, and mix the resulting mixed solution with the concentrated Chinese herbal medicine solution.

[0101] (4) Install the transfer pump in advance, turn on the transfer pump to filter the above solution through 1.0μm and 0.60μm microporous filter membrane titanium rods into stainless steel barrels, and the solution is obtained.

[0102] In this comparative example, the herbal composition was treated using the traditional water extraction and alcohol precipitation method.

[0103] Comparative Example 4

[0104] A traditional Chinese medicine composition with anti-wrinkle effects is made from the following raw materials by weight:

[0105]

[0106] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0107] (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, place them in a steam explosion machine and steam explosion at 1.15 MPa for 2 minutes with saturated steam, then depressurize to obtain depressurized material;

[0108] (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction at 20°C for 1 hour to obtain filtrate and filter residue; the solvent used for countercurrent extraction is an aqueous solution.

[0109] (3) According to the mass ratio of filter residue to water of 1:10, add water to the filter residue obtained in step (2) and ultrasonically treat it at a power of 100W for 0.5h, and filter to obtain filter residue liquid.

[0110] (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol to the concentration of 70wt% for alcohol precipitation. Let it stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to 1.2 (about 60℃) to obtain the concentrated Chinese herbal medicine.

[0111] (5) Dissolve artificial bezoar and borneol in 0.8L of 1,3-propanediol, then add pearl extract (equivalent to 3g of pearl) and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence. Finally, add 4L of 1,3-propanediol and deionized water and mix well. After filtering with titanium rods and 1.0μm and 0.60μm microporous filter membranes in sequence, the Chinese herbal medicine extract is obtained.

[0112] In this comparative example, countercurrent extraction was performed using water, without Tween 80.

[0113] Experimental Example 1

[0114] The anti-wrinkle effects of the herbal extracts prepared in Examples 1-3 and Comparative Examples 1-4 were observed using biochemical methods to scavenge DPPH free radicals, hydroxyl radicals, and superoxide anions. The products prepared in Examples 1-3 were experimental groups a, b, and c, respectively, and the products prepared in Comparative Examples 1-4 were experimental groups d, e, f, and g, respectively.

[0115] 1. Experimental reagents

[0116] The nitrogen free radical (DPPH) scavenging ability kit (batch number 20221130) was purchased from Shanghai Congyi Biotechnology Co., Ltd.; the hydroxyl free radical scavenging rate detection kit (Feton colorimetric method, batch number N18HR201184) was purchased from Shanghai Yuanye Biotechnology Co., Ltd.; and the superoxide anion scavenging ability test kit (micro method, batch number N579966W) was purchased from Shanghai Enzyme-Linked Biotechnology Co., Ltd.

[0117] 2. Experimental Methods

[0118] 2.1 DPPH free radical scavenging

[0119] The experiment was divided into a blank control group (solvent control, 50% ethanol) and experimental groups a–g. 100 μL of each sample was pipetted into a 96-well plate, 100 μL of DPPH solution was added, and the mixture was stirred and kept at room temperature in the dark for 30 min. The absorbance (A) was measured at 517 nm. The absorbance of the blank control group was set as A0, the absorbance of each sample with DPPH solution was set as the test sample (A1), and the absorbance of each sample with sample dilution was set as the control sample (A2). The sample absorbance was then A(A1-A2). The clearance rate was calculated using the formula: Clearance rate (%) = (A0 – A) / A0 × 100.

[0120] 2,2-hydroxyl radical scavenging

[0121] The experiment was divided into a blank control group (solvent control, double-distilled water) and experimental groups a to g. The experimental procedures were strictly followed according to the kit instructions, and the absorbance (A) was measured at 536 nm. Absorbance values ​​were set for the blank tube (A0), undamaged tube (A1), damaged tube (A2), control tube (A3), and test tube (A4). The clearance rate was calculated using the formula: Clearance rate (%) = [(A4 - A3) - (A2 - A0)] / (A1 - A2) × 100.

[0122] 2.3 Superoxide anion scavenging

[0123] The experiment was divided into a blank control group (solvent control, double-distilled water) and experimental groups a–g. The experimental procedures were strictly followed according to the kit instructions, and the absorbance (A) was measured at 530 nm. The absorbance value of the blank tube (A0) was set, and the absorbance value of the measured tube (A) was also set. The clearance rate was calculated using the formula: Clearance rate (%) = (A0 – A) / A0 × 100.

[0124] 3. Experimental Results

[0125] 3.1 Effect on DPPH free radical scavenging rate

[0126] The results of DPPH free radical scavenging ability show that the DPPH free radical scavenging rate of the products prepared in the examples is ≥90%, and the product prepared in Example 1 reaches the maximum DPPH free radical scavenging rate (96.24±2.69)%, with a better anti-wrinkle effect. In contrast, the DPPH free radical scavenging rates of Comparative Examples 1-4 are relatively low, and the anti-wrinkle effect is insufficient.

[0127] Table 1 shows the effect of DPPH free radical scavenging rate. n=9)

[0128] Experimental group a 96.24±2.69 Experimental group b 93.89±1.51 Experimental group c 94.21±3.35 experimental group d 81.47±1.02 Experimental group e 75.53±0.81 Experimental group f 79.38±2.11 experimental group g 85.29±1.98

[0129] 3.2 Effect on hydroxyl radical scavenging rate

[0130] The results of the hydroxyl radical scavenging ability showed that the hydroxyl radical scavenging rate of the products prepared in the examples was ≥85%, and the product prepared in Example 1 achieved the maximum hydroxyl radical scavenging rate (90.50±5.89)%, with a better anti-wrinkle effect. In contrast, the hydroxyl radical scavenging rates of Comparative Examples 1-4 were relatively low, and the anti-wrinkle effect was insufficient.

[0131] Table 2 shows the effect on hydroxyl radical scavenging rate. n=9)

[0132] Experimental group a 90.50±5.89 Experimental group b 89.43±6.33 Experimental group c 87.99±3.03 experimental group d 66.40±5.89 Experimental group e 51.54±6.21 Experimental group f 74.87±4.41 experimental group g 81.64±5.65

[0133] 3.3 Effect on superoxide anion scavenging rate

[0134] The results of superoxide anion scavenging ability show that the superoxide anion free radical scavenging rate of the products prepared in the examples is ≥85%, and the product prepared in Example 1 reaches the maximum superoxide anion free radical scavenging rate (91.12±1.97)%, with a better anti-wrinkle effect. However, the superoxide anion free radical scavenging rates of Comparative Examples 1-4 are relatively low, and the anti-wrinkle effect is insufficient.

[0135] Table 3 shows the effect on superoxide anion scavenging rate. n=9)

[0136] Experimental group a 91.12±1.97 Experimental group b 88.40±2.26 Experimental group c 90.66±3.88 experimental group d 73.25±2.22 Experimental group e 65.67±4.32 Experimental group f 76.81±2.56 experimental group g 82.98±3.54

[0137] Experimental Example 2

[0138] The anti-wrinkle effect of the Babao anti-wrinkle extract prepared in Example 1 at different concentrations was observed using an in vitro human fibroblast photoaging experiment.

[0139] 1. Modeling Method

[0140] Logarithmically growing human skin fibroblasts (HSF) were harvested, digested with trypsin, centrifuged, resuspended, and counted. They were then seeded into 96-well or 48-well plates. After adherence and growth on the second day, 8-methoxypsoralen (8-MOP) was added and the cells were treated for 6 hours. Finally, the cells were irradiated with ultraviolet light for 1 hour in a sterile laminar flow hood to prepare a skin photoaging model.

[0141] 2. Experimental Grouping

[0142] The study was divided into three groups: (1) Normal control group: no treatment was given; (2) Skin photoaging model group: 8-MOP and ultraviolet irradiation were used for 7 hours; (3) Babao anti-wrinkle extract intervention group: 8-MOP and ultraviolet irradiation were used for 7 hours, and then different concentrations of Babao anti-wrinkle extract were added for 48 hours.

[0143] 3. Detection indicators

[0144] 3.1 Cell viability

[0145] At the end of the experiment, 10 μL of CCK-8 solution was added to each well, mixed by shaking, and incubated at 37℃ in a CO2 incubator for 3 hours. The absorbance (A) value of each well was measured at 450 nm using a microplate reader, and the survival rate was calculated. Survival rate (%) = (OD value of experimental group - OD value of zeroing well) / (OD value of control group - OD value of zeroing well) × 100%

[0146] 3.2 β-galactosidase staining

[0147] At the end of the experiment, the cells were gently washed with PBS, and fixative was added sequentially according to the kit instructions. The cells were fixed at room temperature for 15 minutes, washed twice with PBS, and then β-galactosidase staining working solution was added. The cells were incubated overnight at 37°C. The staining results of each experimental group were observed under an inverted phase-contrast optical microscope. The cytoplasm of senescent cells was stained blue. At least 500 cells were counted, and the percentage of positive cells was calculated.

[0148] 3.3 Type I collagen and matrix metalloproteinase levels

[0149] At the end of the experiment, cell supernatant was collected, and the levels of type I collagen and matrix metalloproteinases (MMP-1 and MMP-3) were measured strictly according to the ELISA kit instructions.

[0150] 4. Experimental Results

[0151] 4.1 Viability of photoaged HSF cells

[0152] The anti-wrinkle extract of Babao was diluted to three concentrations: 0.8, 1.6, and 3.2 mg / mL. Cell viability at each concentration was detected using the CCK-8 assay. The results showed that compared with the normal control group, the cell viability in the model group was (54.76±5.32)%, with a statistically significant difference (P<0.01). Compared with the model group, cell viability increased at the three concentrations of 0.8, 1.6, and 3.2 mg / mL, reaching (82.63±15.03)%, (89.27±7.53)%, and (94.63±3.91)%, respectively. (See details below.) Figure 1 .

[0153] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation can lead to a decrease in the survival rate of photo-aged HSF cells, while Babao anti-wrinkle extract exerts a protective effect by increasing the survival rate of photo-aged HSF cells.

[0154] 4.2 β-galactosidase staining to observe the protective effect on photoaged HSF cells

[0155] β-galactosidase staining was used to detect the senescence of photoaged HSF cells. Cells were counted at 500 cells per cell under a light microscope. Normal cells remained unstained, while the cytoplasm of senescent cells appeared blue, representing positive staining cells (see [link to image]). Figure 2 A). The results showed that compared with the normal control group, the percentage of positive cells in the model group was (72.93±6.74)%, and the difference was statistically significant (P<0.01). Compared with the model group, the percentages of positive cells in the low, medium, and high dose groups of Babao Anti-wrinkle Formula were (66.33±6.50)%, (49.53±12.41)%, and (15.20±5.48)%, respectively (see A). Figure 2 B), and there was a statistically significant difference between the medium-dose group (P < 0.05) and the high-dose group (P < 0.01).

[0156] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation can increase the expression of β-galactosidase in HSF cells, leading to HSF cell aging. Babao anti-wrinkle extract inhibits photo-aged HSF cell aging by reducing the expression of β-galactosidase.

[0157] 4.3 Repair function of photoaged HSF cells

[0158] The levels of type I collagen, MMP-1, and MMP-3 in the supernatant of photoaged HSF cells were detected by ELISA. The results showed that compared with the normal control group (7.11±1.56) ng / mL, the level of type I collagen in the model group was significantly reduced to (4.41±1.83) ng / mL, and the difference was statistically significant (P<0.05). Figure 3 A). The high-dose group of Babao anti-wrinkle extract showed a significant increase in type I collagen levels, which was (7.57±1.72) ng / mL, and the difference was statistically significant (P<0.05).

[0159] Compared with the normal control group, MMP-1 (14.76±1.11) ng / mL and MMP-3 (20.76±3.75) ng / mL, the model group showed significantly lower levels of MMP-1 and MMP-3, at (7.76±1.09) ng / mL and (9.17±3.09) ng / mL, respectively, with statistically significant differences (P<0.01). Figure 3 B). Compared with the model group, the MMP-1 levels in the low, medium, and high dose groups of Babao anti-wrinkle extract were significantly increased, at (10.69±1.58) ng / mL, (11.92±0.86) ng / mL, and (12.79±0.90) ng / mL, respectively, with statistically significant differences (P<0.01). The MMP-3 level in the high dose group of Babao anti-wrinkle extract was significantly increased, at (18.46±4.19) ng / mL, with statistically significant differences (P<0.05).

[0160] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation inhibits the synthesis and secretion of type I collagen, MMP-1, and MMP-3 in photo-aged HSF cells, while the Babao anti-wrinkle extract promotes the synthesis and secretion of type I collagen, MMP-1, and MMP-3 in photo-aged HSF cells, thereby exerting a skin repair effect.

[0161] The applicant declares that the technical solution of this invention is illustrated through the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of individual raw materials in the product of this invention, additions of auxiliary components, and selection of specific methods, all fall within the protection scope and disclosure scope of this invention.

Claims

1. A traditional Chinese medicine composition with anti-wrinkle effects, characterized in that, Made from the following parts by weight of raw materials:

2. The herbal composition with anti-wrinkle effects according to claim 1, characterized in that, Made from the following parts by weight of raw materials:

3. The herbal composition with anti-wrinkle effects according to claim 1, characterized in that, Made from the following parts by weight of raw materials:

4. A method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 3, characterized in that, Includes the following steps: (1) Take ginseng, kudzu root, poria cocos, polygonatum odoratum and angelica sinensis by weight, put them in a steam explosion machine for steam explosion treatment, and then depressurize to obtain depressurized material; (2) The pressure relief material obtained in step (1) is subjected to countercurrent extraction to obtain filtrate and filter residue; (3) Add water to the filter residue obtained in step (2) and treat it under ultrasound, then filter to obtain filter residue liquid; (4) Take the filtrate obtained in step (2) and the residue obtained in step (3) and mix them together. Then add ethanol for alcohol precipitation, let stand and filter. Recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste. Then continue to concentrate under reduced pressure to obtain concentrated Chinese herbal medicine. (5) Dissolve artificial bezoar and borneol in a solvent, then add pearl and the concentrated Chinese herbal medicine solution obtained in step (4) in sequence, and finally add solvent and deionized water to mix well. After filtration, the Chinese herbal medicine extract is obtained.

5. The preparation method according to claim 4, characterized in that, In step (1), saturated steam is used for steam explosion and the steam is treated at 1.1-1.2 MPa for 1-3 minutes.

6. The preparation method according to claim 4, characterized in that, In step (2), the countercurrent extraction temperature is 20-25℃, the countercurrent extraction time is 10-30 min, and the countercurrent extraction solvent is an aqueous solution containing 1-1.2 g / L Tween 80.

7. The preparation method according to claim 4, characterized in that, In step (3), the ultrasonic treatment power is 80-120W, the ultrasonic treatment time is 0.5-5h, and the mass ratio of filter residue to water is 1:8-15.

8. The preparation method according to claim 4, characterized in that, In step (4), the ethanol concentration of the alcohol precipitation is 50-70 wt%; the concentration is carried out under reduced pressure to a relative density of 1.15-1.

25.

9. The preparation method according to claim 4, characterized in that, In step (5), the solvent is one or more of butanediol, 1,3-propanediol or glycerol; the filtration is carried out sequentially using 1.0 μm and 0.60 μm microporous filter membrane titanium rods.

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