梨PcBBX24C544T单碱基突变及其矮化调控应用

By isolating and overexpressing the PcBBX24C544T gene in 'Red Pear', the shortcomings of the pear tree dwarfing mechanism were solved, realizing dwarfing breeding and efficient dwarfing cultivation of fruit trees, and providing new gene resources and methods.

CN117802111BActive Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2023-10-09
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The underlying mechanisms of dwarfing pear trees are poorly understood in current technologies, and there is a lack of effective genetic resources and methods to achieve dwarfing cultivation of fruit trees.

Method used

The PcBBX24C544T gene from 'Red Pear' was isolated and cloned. This gene lost its C-terminal domain due to a single-base mutation. Overexpression of this gene resulted in a dwarfing phenotype in the plant. The gene overexpression was achieved by constructing a recombinant expression vector and genetically engineered bacteria.

Benefits of technology

It provides new genetic resources for dwarfing fruit tree breeding, reduces agricultural costs, enables high-density cultivation, meets consumer demand, and stably obtains dwarf plants through Agrobacterium-mediated genetic transformation.

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Abstract

本发明公开了梨PcBBX24C544T单碱基突变及其矮化调控应用。一种分离自‘红巴梨’具有促进梨树植株矮化生长的转录因子PcBBX24C544T,属于B‑Box家族成员,其核苷酸序列为SEQ IDNo.1所示,其编码的氨基酸序列为序列表SEQ ID No.2所示。通过农杆菌介导遗传转化方法将PcBBX24C544T基因稳定转化大叶烟草。经分子生物学功能验证,表明本发明克隆的PcBBX24C544T基因具有调控植株矮化生长的功能。该基因的发现,为分子育种提供更高效地途径,为矮化树型的分子育种提供新的基因资源,为实施绿色农业提供新的遗传资源,该遗传资源的开发利用有利于降低农业成本和实现环境友好。
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