A device for separating and extracting CGF and a method for extracting CGF

Abstract

The application discloses a CGF separation and extraction device and an extraction method thereof. The device comprises a CGF collecting part, a sealing upper cover, a preparation cavity, a separation piston, a push-up supporting part, a lower cover and a push-up screw member. The preparation cavity comprises a separation chamber at the upper part and a blood storage chamber at the lower part. The sealing upper cover is installed on the separation chamber. The CGF collecting part is detachably installed in the sealing upper cover. The push-up supporting part is combinedly installed below the separation piston. The lower cover is installed at the lower end opening of the blood storage chamber. The push-up screw member is installed in the push-up guide hole at the lower end surface of the lower cover through thread connection. The application has the advantages that the structure design is reasonable, the multiple sealing combination forms reliable sealing effect, the separation is accurate, the preparation efficiency is high, the target product is safe and pollution-free, the recovery and treatment are convenient, and the like. The extraction method does not add any additive, continuous differential centrifugation is adopted, more effective substances with higher concentration are obtained, and the operation is convenient and efficient.

Description

Technical Field

[0001] This invention relates to the field of medical laboratory equipment technology, and more specifically, to a CGF separation and extraction device and extraction method thereof. Background Technology

[0002] CGF (Concentrated Growth Factors) is an abbreviation for concentrated growth factors. It is a latest-generation platelet concentrate extracted from blood through differential centrifugation, containing multiple growth factors. CGF contains various growth factors, including platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), and insulin-like growth factor (IGF-1), among others. These growth factors have high biological activity, thus CGF plays an important role in tissue repair and regeneration.

[0003] Compared to first-generation platelet concentrate—platelet-rich plasma (PRP), CGF does not require the addition of thrombin, thus avoiding the possibility of allergic reactions and rejection in patients. Compared to second-generation platelet concentrate—platelet-rich fibrin (PRF), CGF has a denser network structure, and its slow release of growth factors more closely resembles the natural process of tissue healing. CGF has a wide range of applications, including dental implantology, plastic surgery, and dermatology, and has broad application prospects.

[0004] The preparation of CGF requires drawing blood and transferring it to a separation device, then centrifuging it using a specific centrifugation program to separate the blood into three layers: the bottom layer is blood cells, the middle layer is CGF, and the top layer is anemic platelet plasma (PPP). The CGF layer located in the middle of the separation solution is then collected by extraction.

[0005] The commonly used preparation method is to first place the blood to be prepared into a centrifuge tube and centrifuge it. After centrifugation, the blood that has been separated into layers is extracted into the centrifuge tube in sequence using a syringe for later use.

[0006] However, existing preparation methods still have many operational problems. First, since the CGF after centrifugation is located in the middle of the separating solution, when extracting CGF, the needle needs to penetrate the upper PPP layer before entering the CGF layer. After puncturing into the CGF layer, the outer wall of the needle will be covered with the upper PPP layer, which will affect the purity of CGF during extraction. In addition, since PPP, CGF, and red blood cells are still in the same container, a certain amount of PPP or red blood cells may be extracted during the CGF extraction process, and there is a risk of remixing the separated blood, directly affecting the quality and efficacy of CGF.

[0007] Secondly, after preparation, CGF needs to be extracted using a syringe for injection or other clinical treatment. This step not only reduces work efficiency but also poses a risk of secondary contamination, affecting the biosafety of CGF.

[0008] Finally, the existing preparation process is time-consuming. In order to ensure that the blood can remain in a liquid state during the preparation process to complete the centrifugation and separation process, an anticoagulant needs to be added during the separation preparation to avoid blood coagulation affecting the centrifugation stratification effect. However, adding an anticoagulant will increase the possibility of exogenous substances reacting with the human body and causing harm to the human body, affecting the stability and safety of the prepared CGF.

[0009] A search revealed that patent document CN106176214B, entitled "A Blood Separation and Extraction Device for Extracting Platelet-Rich Growth Factors," discloses a structure comprising: a receiving space at the lower part of a main tube for containing blood; a passageway with an inclined portion and an open top, narrower in inner diameter than the lower portion, formed at the upper part of the receiving space; a separation space containing a portion of the separated blood layer obtained by centrifugal separation through the passageway; a slider slidably attached to the receiving space, pushing the separated blood layer obtained by centrifugal separation towards the separation space via the passageway; and a detachable upper cover attached to the upper end of the main tube. A connecting portion is formed at the lower center, connecting with the upper end of the passageway when attached to the main tube, and includes a storage portion extending downwards towards the connecting portion. This prior art has a complex structure, resulting in difficult, time-consuming, and inaccurate separation, collection, and transfer operations.

[0010] The patent document with announcement number CN203678548U, entitled "A Device for Extracting High-Concentration Platelet-Rich Plasma," discloses a structure comprising a collection tube, a pipette, and a connecting sleeve. The collection tube consists of a push rod, a sleeve, and a head protective sleeve. The push rod is slidably located inside the sleeve. One end of the head protective sleeve is open, and the other end is closed. The open end of the head protective sleeve can be fixed to the interface of the sleeve. The pipette consists of a tube body and a connecting port. The tube body is hollow inside, with one end closed and the other end being the connecting port, which can be fixed to the interface of the sleeve. The connecting sleeve consists of an upper interface, a lower interface, and a handle. The upper and lower interfaces are interconnected, and the upper interface can be fixed to the interface of the sleeve. When using this structure for platelet-rich plasma extraction, the pipette needs to be removed, and then connected to a regular syringe via the combined sleeve before collection can be performed. After collection, it needs to be reassembled with the pipette to transfer the target substance. The collection and transfer process requires multiple disassembly and reassembly steps, which is inconvenient to operate and has low transfer efficiency. Moreover, the collected target material is easily contaminated during the multiple disassembly and reassembly steps, which affects the usability of the extracted target material.

[0011] Furthermore, there is currently no unified standard for the preparation and extraction of CGF, and all methods have certain drawbacks, including: the isochronous centrifugation procedure used during separation can lead to insufficient platelet activation, making it impossible to obtain higher concentrations of growth factors and fibrin; after centrifugation in centrifuge tubes, the caps of the blood collection tubes need to be removed, exposing the separated blood to air and posing a risk of contamination; existing methods have small blood collection volumes, resulting in a small final product volume that cannot meet practical needs; the separation and extraction process is time-consuming and requires the addition of anticoagulants, which may cause allergic reactions and pain in patients during subsequent use; after centrifugation, the injection needle needs to be inserted into the middle of the separation liquid, and after puncturing into the CGF layer, the outer wall of the needle will be covered with the upper layer of PPP; in addition, since PPP, CGF, and red blood cells are still in the same system, a certain amount of PPP or red blood cells may be extracted during the extraction of CGF, directly affecting the quality and efficacy of the extracted CGF.

[0012] In view of this, there is a need to develop a preparation device and extraction method that is easy to operate, has high preparation efficiency, and produces safe and pollution-free target products, so as to meet the current requirements for the extraction, preparation and use of CGF. Summary of the Invention

[0013] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a CGF separation and extraction device that has a reasonable structural design, is easy to use, provides accurate separation, has high preparation efficiency, and ensures that the target product is safe and pollution-free.

[0014] This invention also provides an extraction method for a CGF separation and extraction device that can achieve complete blood separation without the need for any additives and is convenient and efficient in extraction operation.

[0015] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A CGF separation and extraction device includes a CGF collection section, a sealing top cover, a preparation chamber, a separation piston, a lifting support section, a lower cover, and a lifting spiral component. The preparation chamber includes an upper separation chamber and a lower blood containing chamber, which are connected via a chamber channel. The sealing top cover is mounted on the separation chamber, and the CGF collection section is installed inside the sealing top cover. The lifting support section is assembled and installed below the separation piston. The separation piston and the lifting support section are slidably and sealingly mounted within the blood containing chamber. The lower cover is installed at the lower opening of the blood containing chamber, and the lifting spiral component is threadedly connected to the lower end face of the lower cover. Inside the push guide hole, the upper end of the push spiral component and the lower end of the push support part cooperate to collect CGF in the separated liquid after centrifugation. The blood containing chamber is used to store blood during the preparation process, and the separation chamber is used to separate and store the separated liquid after centrifugation. The push spiral component cooperates with the separation piston and the push support part to smoothly push and separate the separated liquid after centrifugation. The separated liquid that has completed the stratification after centrifugation and is stored in the blood containing chamber is pushed by the push spiral component and the lower cover to push the push support part to make the separation piston rise smoothly inside the blood containing chamber. This allows the layers of the separated liquid to be separated into the separation chamber and the CGF collecting part according to the requirements through the chamber channel.

[0016] Preferably, the CGF collection section includes a CGF collection cylinder, a separation extraction plug, and a separation extraction operating rod. The separation extraction plug is assembled at the lower end of the separation extraction operating rod and is slidably installed on the inner wall of the CGF collection cylinder. The lower end of the CGF collection cylinder has an extraction port, and the outer wall of the CGF collection cylinder has an external thread. The separation extraction operating rod and the separation extraction plug work together to directly extract the centrifugally separated CGF from the extraction port into the CGF collection cylinder. The sealed top cover has a CGF collection section receiving cavity inside. The bottom of the CGF collection section receiving cavity has a separation connection port, which is connected to the cavity channel section. When the separation connection port is connected to the cavity channel section, a sealed storage structure is formed inside the CGF collection section receiving cavity. The upper end of the inner wall of the CGF collection chamber is provided with a cylindrical body assembly internal thread. The CGF collection part is installed in the CGF collection chamber through the cylindrical body assembly external thread and the cylindrical body assembly internal thread in a threaded connection. The upper end of the chamber channel is provided with a extraction port assembly groove. The extraction port extends into the interior of the separation connection port. The front end of the extraction port is inserted into the extraction port assembly groove to maintain the same sealing performance inside the CGF collection cylinder and the blood collection chamber after being connected and assembled. After the extraction port and the extraction port assembly groove are connected and embedded, the separated CGF is extracted into the CGF collection cylinder from the separation connection port. The upper outer wall of the separation chamber is provided with an external thread for the upper cover assembly, and the inner wall of the sealing upper cover is provided with an internal thread for the upper cover assembly. The sealing upper cover is rotatably mounted above the separation chamber through the threaded connection between the internal thread and the external thread of the upper cover assembly. The combination of the separation chamber and the sealing upper cover is provided with a double-layer sealing structure, which provides sufficient sealing effect when the sealing upper cover and the separation chamber are combined. The outer wall of the piston is provided with a sealing protrusion ring, which is fitted against the inner wall of the blood containing chamber with an interference fit. The lower end of the outer wall of the blood containing chamber is provided with an external thread for the lower cover assembly, and the inner wall of the lower cover is provided with an internal thread for the lower cover assembly. The lower cover is installed below the blood containing chamber through a threaded connection between the internal thread and the external thread of the lower cover assembly. The inner wall of the push guide hole is provided with a push internal thread, and the outer wall of the push connecting rod of the push spiral component is provided with a push external thread. The push spiral component is installed and lowered within the lower cover through a threaded connection between the external thread and the internal thread of the push. The lower cover is used to limit the installation position of the push support and cooperate with the push spiral component to achieve the push separation function. The upper end of the push connecting rod is provided with a push embedding protrusion, and the push embedding protrusion and the push assembly groove at the lower end of the push support are embedded and mated together. The bottom of the push support is provided with an inclined injection hole.

[0017] Preferably, a sealing protrusion is provided above the chamber channel, and a sealing groove is provided below the separation connection port, with the sealing protrusion embedded in the sealing groove. The sealing protrusion and the sealing groove, after being embedded and combined, further improve the separation and sealing effect of the CGF collection chamber.

[0018] Preferably, the double-layer sealing structure includes an upper cover sealing ring installed below the external thread of the upper cover assembly and an upper cover assembly sealing ring disposed on the inner end face of the upper cover. The upper cover sealing ring is in a tight fit with the inner wall of the upper cover, and the upper cover sealing ring maintains the combined sealing degree between the upper cover and the separation chamber when the upper cover rotates on the separation chamber. The upper cover assembly sealing ring is in a tight fit with the top of the upper opening of the separation chamber, and the upper cover assembly sealing ring ensures a tight fit between the upper cover and the separation chamber when the upper cover rotates and fits against the upper end of the separation chamber.

[0019] Preferably, the lower end of the separating piston is provided with a combination hole, the interior of the combination hole is provided with a combination limiting groove, the upper end of the lifting support is provided with a piston combination protrusion, the top of the piston combination protrusion is provided with a combination limiting flange, the piston combination protrusion is assembled and installed inside the combination hole, and the combination limiting flange is embedded in the combination limiting groove, so that the separating piston and the lifting support are firmly combined together.

[0020] Preferably, the lower end of the lifting screw component is provided with a lifting grip portion, and the outer wall of the lifting grip portion is provided with a lifting grip locking protrusion. The lifting grip portion is used to provide the operator with sufficient gripping area when rotating the lifting screw component. The array of lifting grip locking protrusions is arranged on the outer wall of the lifting grip portion. The lifting grip locking protrusions cooperate with the lifting grip portion to allow the operator to more conveniently grip and rotate the lifting screw component to complete the lifting and separation operation smoothly and accurately according to the separation requirements.

[0021] Preferably, the upper surface of the sealing cover is provided with a PPP extraction section and a chamber pressure regulating section. The PPP extraction section allows the operator to selectively extract the separated PPP for effective utilization after completing the separation of CGF. The chamber pressure regulating section is used to assist in regulating the air pressure in the separation chamber.

[0022] Preferably, the upper surface of the sealed cover is provided with a PPP extraction section and a chamber pressure regulating section. The PPP extraction section allows the operator to selectively extract the separated PPP for further effective use after completing the separation of CGF. The chamber pressure regulating section helps maintain the pressure balance inside and outside the separation chamber.

[0023] Preferably, the PPP extraction unit includes a PPP extraction hole and an extraction hole sealing plug installed on the PPP extraction hole. During centrifugation, the extraction hole sealing plug is tightly inserted into the PPP extraction hole to keep the separation chamber sealed. After centrifugation separation and extraction, when it is necessary to utilize the separated PPP resources, the remaining PPP in the separation chamber is extracted and utilized by passing through the extraction hole sealing plug.

[0024] Furthermore, the chamber pressure regulating unit includes a breathable membrane fixing rubber plug, a pressure regulating breathable membrane, a pressure regulating hole, and a pressure regulating mounting groove. The pressure regulating hole is located in the middle of the pressure regulating mounting groove. The pressure regulating breathable membrane is fitted to the bottom of the pressure regulating mounting groove. The breathable membrane fixing rubber plug is installed inside the pressure regulating mounting groove, and the breathable membrane fixing rubber plug has a top cover pressure regulating hole in its middle. The pressure regulating breathable membrane is a waterproof and breathable membrane. The cooperation between the pressure regulating breathable membrane and the top cover pressure regulating hole and pressure regulating hole maintains the air pressure balance inside and outside the separation chamber during storage and sterilization.

[0025] Preferably, the separation chamber and blood containing chamber of the preparation cavity, as well as the CGF collection cylinder of the CGF collection part, are made of transparent material, allowing the operator to clearly observe the preparation process within the preparation cavity and CGF collection part during operation, and accurately complete the CGF separation and preparation operation. The outer walls of the blood containing chamber and the CGF collection cylinder are provided with volume markings, allowing the operator to accurately control the prepared dosage according to requirements. An operating grip is provided above the separation and extraction operating rod, and the operating grip has recesses on both sides, making it easier for the operator to perform CGF separation and extraction operations.

[0026] Preferably, a sealing cap is installed at the upper opening of the CGF collection chamber. After the CGF separation and preparation are completed, the CGF collection part is separated and taken out, and the sealing cap is placed on the upper opening of the CGF collection chamber to maintain the airtightness of the CGF collection chamber, which facilitates recycling and avoids environmental pollution during recycling.

[0027] Furthermore, the present invention provides an extraction method for a CGF separation and extraction device, comprising the following steps: Step 1: Inject the blood collected as needed into the blood receiving chamber through the injection port at the bottom of the lifting support; Step 2: Select equal weights of CGF separation and extraction devices for balancing and place them simultaneously into a centrifuge. Use the dedicated differential centrifugation program prepared for CGF for differential centrifugation. The dedicated differential centrifugation program is as follows: acceleration for 30 seconds; centrifugal acceleration of 692g for 2 minutes; centrifugal acceleration of 547g for 4 minutes; centrifugal acceleration of 692g for 4 minutes; centrifugal acceleration of 855g for 3 minutes; deceleration for 36 seconds. Step 3: After centrifugation, screw the top cover up to separate the separation port from the chamber channel, leaving a separation gap; Step 4: By turning the lifting screw component installed on the lower cover, the lifting connecting rod and the separation piston are moved upward, transferring the upper layer of the separated blood, PPP (platelet-rich plasma), to the separation chamber; Step 5: Twist the top cover down to fully align the separation connector with the chamber channel, connecting the CGF collection cylinder to the blood containment chamber; Step 6: Continue to rotate the lifting screw component installed on the lower cover to move the lifting connecting rod and the separation piston upward, so as to completely transfer the target product CGF (concentrated growth factor) to the CGF collection section; Step 7: Hold and twist the CGF collection cylinder upwards to unscrew the CGF collection part from the CGF collection part receiving cavity, store the CGF collection part containing the target product CGF for later use, and complete the extraction and collection.

[0028] Compared with the prior art, the advantages of this invention are: 1. The CGF separation and extraction device provided by this invention directly assembles the CGF collection section and the preparation chamber together, resulting in a robust assembly that facilitates blood storage. After assembly, the device can be directly placed in a centrifuge for centrifugation, making it convenient to use. The CGF collection section, preparation chamber, and sealing cap form an integrated, detachable structure, allowing for direct CGF preparation after separation, thus improving work efficiency. Furthermore, the prepared CGF can be directly used for clinical injection without additional syringe puncture and extraction steps, reducing syringe consumption, minimizing the time spent on syringe puncture and extraction, and avoiding secondary contamination of the target preparation by external syringes.

[0029] 2. The CGF separation and extraction device provided by the present invention adopts a multi-combination sealing structure by using sealing auxiliary connections at the connection and combination parts of each component, which effectively ensures that the internal chamber of the device maintains a reasonable sealing effect during the storage, centrifugation, separation and preparation of blood, and avoids external contamination of the separated and prepared CGF.

[0030] 3. The CGF separation and extraction device provided by the present invention, with the combination of the lifting spiral component and the lifting support, and the guidance of the lower cover, can smoothly and steadily lift the separation liquid, achieving accurate separation and rapid preparation of the required CGF.

[0031] 4. The structure of the CGF separation and extraction device provided by this invention provides convenient and rapid preparation operations. The entire extraction and separation preparation process can be effectively controlled and completed within 30 minutes. No anticoagulant needs to be added to the blood during separation preparation, which can reduce the harm of exogenous substances to the human body. It has the advantages of convenient preparation and high efficiency.

[0032] 5. The separation chamber, blood containment chamber, and CGF collection cylinder of the preparation chamber of the CGF separation and extraction device provided by the present invention are all made of transparent material, which allows the preparation operator to more quickly and intuitively grasp the storage and separation status in the preparation chamber and the preparation status in the CGF collection cylinder, and to more accurately control the dosage of CGF according to the preparation requirements. After separation, the required CGF solution can be directly prepared.

[0033] 6. The CGF separation and extraction device provided by this invention is also equipped with a sealing cap. After the preparation is completed, the CGF collection part is disassembled and stored for later use, maintaining the complete seal of the CGF collection part receiving cavity and the preparation cavity. This prevents the remaining liquid after preparation from leaking into the device cavity, ensuring that the used CGF separation and extraction device is handled in accordance with regulations, avoiding environmental pollution after recycling. At the same time, this device has the advantages of reasonable structural design, high innovation, multiple sealing combinations to form a reliable sealing effect, accurate separation, high preparation efficiency, safe and pollution-free target product, and convenient recycling.

[0034] 7. The extraction method provided by this invention is convenient and efficient. Without adding any additives, the extraction method relies on physical acceleration and deceleration to fully activate the α-granules in platelets through physical collision, thereby releasing more growth factors and other effective substances (fibrin, CD34 positive cells, leukocytes, etc.) to obtain a higher concentration of effective substances (growth factors, immune proteins, etc.). Combined with the fact that this device can hold 20ml (maximum 25ml) of peripheral venous blood, the yield of the target product can be significantly improved, and the extraction effect can be better enhanced. Attached Figure Description

[0035] Figure 1 This is a schematic diagram of the structure of the present invention.

[0036] Figure 2 This is an exploded structural diagram of the present invention.

[0037] Figure 3 This is a schematic diagram of the disassembled state of the present invention.

[0038] Figure 4 This is a schematic diagram of the CGF collection section of the present invention.

[0039] Figure 5 This is a schematic diagram of the structure of the sealing cover of the present invention.

[0040] Figure 6 This is a schematic diagram of the preparation cavity of the present invention.

[0041] Figure 7 This is a schematic diagram of the separation piston of the present invention.

[0042] Figure 8 This is a schematic diagram of the lifting support part of the present invention.

[0043] Figure 9 This is a schematic diagram of the structure of the lower cover of the present invention.

[0044] Figure 10 This is a schematic diagram of the structure of the PPP extraction unit and the chamber pressure regulating unit of the present invention.

[0045] Figure 11 This is a schematic diagram of the separation and preparation process of the present invention.

[0046] Figure 12 This is a schematic diagram of the installation and sealing cap assembly of the present invention.

[0047] Explanation of icon numbers: CGF collection section-1, sealing cover-2, preparation chamber-3, separation piston-4, lifting support-5, lower cover-6, lifting screw component-7, PPP extraction section-8, chamber pressure regulating section-9, CGF collection cylinder-11, separation extraction plug-12, separation extraction operating rod-13, extraction port-14, operating grip-15, inner recess-16, CGF collection section receiving cavity-21, separation connection port-22, docking sealing groove-23, upper cover combination sealing ring-24, installation sealing cap-25, separation chamber-31, blood receiving chamber-32, chamber passage section- 33, Butt sealing protrusion - 34, Top cover sealing ring - 35, Extraction port combination groove - 36, Combination hole - 41, Combination limiting groove - 42, Piston combination protrusion - 51, Combination limiting protrusion - 52, Push-up combination groove - 53, Injection hole - 54, Push-up guide hole - 61, Push-up connecting rod - 71, Push-up embedded protrusion - 72, Push-up grip - 73, Push-up grip locking protrusion - 74, PPP extraction hole - 81, Extraction hole sealing plug - 82, Breathable membrane fixing rubber plug - 91, Pressure regulating breathable membrane - 92, Pressure regulating hole - 93, Top cover pressure regulating hole - 94, Pressure regulating mounting groove - 95. Detailed Implementation

[0048] The present invention will be further described below with reference to specific embodiments. The accompanying drawings are for illustrative purposes only, representing schematic diagrams rather than actual physical objects, and should not be construed as limiting the scope of this patent. To better illustrate the embodiments of the present invention, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0049] In the accompanying drawings of the embodiments of the present invention, the same or similar reference numerals correspond to the same or similar components. In the description of the present invention, it should be understood that if terms such as "upper," "lower," "left," "right," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting the present patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0050] The present invention will be further described below with reference to specific embodiments and accompanying drawings: Figures 1 to 12 These are schematic diagrams of various structures of the present invention. Further, the present invention is a CGF separation and extraction device, including a CGF collection section 1, a sealing upper cover 2, a preparation chamber 3, a separation piston 4, a lifting support section 5, a lower cover 6, and a lifting spiral component 7. The preparation chamber 3 includes an upper separation chamber 31 and a lower blood containing chamber 32, the separation chamber 31 and the blood containing chamber 32 being connected via a chamber channel 33. The sealing upper cover 2 is installed on the separation chamber 31, the CGF collection section 1 is installed inside the sealing upper cover 2, the lifting support section 5 is assembled and installed below the separation piston 4, and the separation piston 4 and the lifting support section 5 are slidably and sealingly installed inside the blood containing chamber 32. The lower cover 6 is installed at the lower opening of the blood containing chamber 32, and the lifting spiral component 7 is threadedly connected to the lower cover 31. Inside the push guide hole 61 on the lower end face of the lower cover 6, the upper end of the push screw component 7 cooperates with the lower end of the push support part 5. The CGF collection part 1 is used to collect CGF in the centrifuged separation liquid. The blood containing chamber 32 is used to store blood during the preparation process. The separation chamber 31 is used to separate and store the centrifuged separation liquid. The push screw component 7 is used to cooperate with the separation piston 4 and the push support part 5 to smoothly push and separate the centrifuged separation liquid. The separation liquid that has completed the stratification after centrifugation and is stored in the blood containing chamber 32 is pushed by the push screw component 7 and the lower cover 6 to push the push support part 5 so that the separation piston 4 rises smoothly inside the blood containing chamber 32. The layers of the separation liquid are separated into the separation chamber 31 and the CGF collection part 1 through the chamber channel part 33 as required.

[0051] Preferably, the CGF collection unit 1 includes a CGF collection cylinder 11, a separation extraction plug 12, and a separation extraction operating rod 13. The separation extraction plug 12 is assembled at the lower end of the separation extraction operating rod 13. The separation extraction plug 12 is slidably installed on the inner wall of the CGF collection cylinder 11. The lower end of the CGF collection cylinder 11 is provided with an extraction port 14. The outer wall of the CGF collection cylinder 11 is provided with an external thread. The separation extraction operating rod 13 and the separation extraction plug 12 work together to directly extract the CGF separated by centrifugation from the extraction port 14 into the CGF collection cylinder 11.

[0052] Furthermore, the sealed top cover 2 has a CGF collection cavity 21 inside, and the bottom of the CGF collection cavity 21 has a separation connection port 22. The separation connection port 22 is connected and combined with the chamber channel 33. When the separation connection port 22 is connected with the chamber channel 33, a sealed storage structure is formed inside the CGF collection cavity 21. The upper end of the inner wall of the CGF collection chamber 21 is provided with a cylindrical body assembly internal thread. The CGF collection part 1 is installed in the CGF collection chamber 21 through the cylindrical body assembly external thread and the cylindrical body assembly internal thread in a threaded connection. The upper end of the chamber channel 33 is provided with an extraction port assembly groove 36. The extraction port 14 extends into the interior of the separation connection port 22. The front end of the extraction port 14 is inserted into the extraction port assembly groove 36 to maintain the same sealing performance inside the CGF collection cylinder 11 and the blood collection chamber 32 after docking and assembly. After the extraction port 14 and the extraction port assembly groove 36 are docked and embedded, the separated CGF is extracted into the CGF collection cylinder 11 from the separation connection port 22.

[0053] Furthermore, the upper end of the outer wall of the separation chamber 31 is provided with an external thread for the upper cover assembly, and the inner wall of the sealing upper cover 2 is provided with an internal thread for the upper cover assembly. The sealing upper cover 2 is rotatably installed above the separation chamber 31 through the threaded connection between the internal thread and the external thread of the upper cover assembly. The combination part of the separation chamber 31 and the sealing upper cover 2 is provided with a double-layer sealing structure. The double-layer sealing structure is used to provide sufficient sealing effect when the sealing upper cover 2 and the separation chamber 31 are combined.

[0054] Furthermore, the outer wall of the piston is provided with a sealing protrusion ring, which fits snugly against the inner wall of the blood containing chamber 32, and the sealing protrusion ring and the inner wall of the blood containing chamber 32 are interference fit. The lower end of the outer wall of the blood containing chamber 32 is provided with a lower cover assembly external thread, and the inner wall of the lower cover 6 is provided with a lower cover assembly internal thread. The lower cover 6 is installed below the blood containing chamber 32 through the threaded connection between the lower cover assembly internal thread and the lower cover assembly external thread. The inner wall of the push guide hole 61 is provided with a push internal thread, and the outer wall of the push connecting rod 71 of the push screw component 7 is provided with a push external thread. The push screw component 7 is installed in the lower cover 6 through the threaded connection between the push external thread and the push internal thread. The lower cover 6 is used to limit the installation position of the push support part 5 and cooperate with the push screw component 7 to realize the push separation function. The upper end of the push-up connecting rod 71 is provided with a push-up embedding protrusion 72, and the push-up embedding protrusion 72 and the push-up combination groove 53 at the lower end of the push-up support part 5 are embedded and connected. The bottom of the push-up support part 5 is provided with an inclined injection hole 54.

[0055] Preferably, a sealing protrusion 34 is provided above the chamber channel portion 33, and a sealing groove 23 is provided below the separation connection port 22. The sealing protrusion 34 is embedded in the sealing groove 23. After the sealing protrusion 34 and the sealing groove 23 are embedded and combined, the separation and sealing effect of the CGF collection portion receiving cavity 21 is further improved.

[0056] Preferably, the double-layer sealing structure includes an upper cover sealing ring 35 installed below the external thread of the upper cover assembly and an upper cover assembly sealing ring 24 provided on the inner end face of the sealing upper cover 2. The upper cover sealing ring 35 is in a sealed fit with the inner wall of the sealing upper cover 2. The upper cover sealing ring 35 maintains the combined sealing degree between the sealing upper cover 2 and the separation chamber 31 when the sealing upper cover 2 rotates on the separation chamber 31.

[0057] Furthermore, the upper cover combined sealing ring 24 is sealed to the top of the upper opening of the separation chamber 31. The upper cover combined sealing ring 24 ensures the tightness and sealing of the upper cover 2 and the separation chamber 31 when the upper cover 2 is rotated and fitted to the upper end of the separation chamber 31.

[0058] Preferably, the lower end of the separating piston 4 is provided with a combination hole 41, the interior of the combination hole 41 is provided with a combination limiting groove 42, the upper end of the lifting support part 5 is provided with a piston combination protrusion 51, the top of the piston combination protrusion 51 is provided with a combination limiting flange 52, the piston combination protrusion 51 is assembled and installed inside the combination hole 41, and the combination limiting flange 52 is embedded in the combination limiting groove 42, so that the separating piston 4 and the lifting support part 5 are firmly combined together.

[0059] Preferably, the lower end of the lifting screw component 7 is provided with a lifting grip portion 73, and the outer wall of the lifting grip portion 73 is provided with a lifting grip locking protrusion 74. The lifting grip portion 73 is used to provide the operator with sufficient gripping area when rotating the lifting screw component 7. The lifting grip locking protrusion 74 is arranged in an array on the outer wall of the lifting grip portion 73. The lifting grip locking protrusion 74 cooperates with the lifting grip portion 73 to allow the operator to more conveniently grip and rotate the lifting screw component 7 to complete the lifting and separation operation smoothly and accurately according to the separation requirements.

[0060] Preferably, the upper surface of the sealing cover 2 is provided with a PPP extraction section 8 and a chamber pressure regulating section 9. The PPP extraction section 8 is used by the operator to selectively extract the separated PPP for effective use after the CGF separation is completed. The chamber pressure regulating section 9 is used to help maintain the air pressure balance inside and outside the separation chamber 31.

[0061] Preferably, the PPP extraction unit 8 includes a PPP extraction hole 81 and an extraction hole sealing plug 82 installed on the PPP extraction hole 81. During centrifugation, the extraction hole sealing plug 82 is tightly inserted into the PPP extraction hole 81 to keep the separation chamber 31 sealed. After centrifugation separation and extraction, when it is necessary to utilize the separated PPP, the remaining PPP in the separation chamber 31 is extracted and utilized by passing through the extraction hole sealing plug 82.

[0062] Preferably, the chamber pressure regulating part 9 includes a breathable membrane fixing rubber plug 91, a pressure regulating breathable membrane 92, a pressure regulating hole 93, and a pressure regulating mounting groove 95. The pressure regulating hole 93 is located in the middle of the pressure regulating mounting groove 95. The pressure regulating breathable membrane 92 is fitted to the bottom of the pressure regulating mounting groove 95. The breathable membrane fixing rubber plug 91 is installed in the pressure regulating mounting groove 95. The breathable membrane fixing rubber plug 91 has a top cover pressure regulating hole 94 in the middle. The pressure regulating breathable membrane 92 is a waterproof and breathable membrane. The cooperation between the pressure regulating breathable membrane 92 and the top cover pressure regulating hole 94 and pressure regulating hole 93 maintains the air pressure balance inside and outside the separation chamber 31 during storage and sterilization.

[0063] Preferably, the separation chamber 31 and blood containing chamber 32 of the preparation chamber 3 and the CGF collecting cylinder 11 of the CGF collecting part 1 are made of transparent material, so that the operator can clearly observe the preparation situation in the preparation chamber 3 and the CGF collecting part 1 during operation, and accurately complete the CGF separation and preparation operation. The outer walls of the blood containing chamber 32 and the CGF collecting cylinder 11 are provided with volume scale markings, so that the operator can accurately control the dosage according to the requirements.

[0064] Furthermore, an operating grip 15 is provided above the separation and extraction operating rod 13, and recesses 16 are provided on both sides of the operating grip 15, making it easier for the operator to perform CGF separation and extraction operations.

[0065] Preferably, a sealing cap 25 is installed at the upper opening of the CGF collection cavity 21. After the CGF separation preparation is completed, the CGF collection part 1 is separated and taken out, and the sealing cap 25 is placed on the upper opening of the CGF collection cavity 21 to maintain the sealing degree inside the CGF collection cavity 21, which facilitates recycling and avoids environmental pollution during recycling.

[0066] Furthermore, the present invention provides an extraction method for a CGF separation and extraction device, comprising the following steps: Step 1: Inject the blood collected as needed into the blood containing chamber 32 through the injection hole 54 at the bottom of the lifting support 5; Step 2: Select equal weights of CGF separation and extraction devices for balancing and place them simultaneously into a centrifuge. Use the dedicated differential centrifugation program prepared for CGF for differential centrifugation. The dedicated differential centrifugation program is as follows: acceleration for 30 seconds; centrifugal acceleration of 692g for 2 minutes; centrifugal acceleration of 547g for 4 minutes; centrifugal acceleration of 692g for 4 minutes; centrifugal acceleration of 855g for 3 minutes; deceleration for 36 seconds. Step 3: After centrifugation, screw the sealing cap 2 to rise, so that the separation connection port 22 separates from the chamber channel 33, leaving a separation gap; Step 4: By turning the lifting screw component 7 installed on the lower cover 6, the lifting connecting rod 71 and the separation piston 4 are moved upward, transferring the upper layer of the separated blood PPP (platelet-rich plasma) to the separation chamber 31; Step 5: Twist the sealing cover 2 to lower it, so that the separation connection port 22 is fully connected with the chamber channel 33, and the CGF collection cylinder 11 is connected to the blood containing chamber 32; Step 6: Continue to rotate the lifting screw component 7 installed on the lower cover 6 to drive the lifting connecting rod 71 and the separation piston 4 upward, so as to completely transfer the target product CGF (concentrated growth factor) to the CGF collection section 1; Step 7: Hold and twist the CGF collection cylinder 11 upwards to unscrew the CGF collection part 1 from the CGF collection part receiving cavity 21, store the CGF collection part 1 containing the target product CGF for later use, and complete the extraction and collection.

[0067] First embodiment: The extraction and preparation process for CGF includes the following steps: 1. Using a 30ml syringe and a blood collection needle / intravenous infusion needle, draw 20ml of peripheral venous blood from the human body. Take out the CGF separation and extraction device provided by this invention, first unscrew the push screw component 7 from the lower end of the lower cover 6, replace the 30ml syringe with a No. 7 injection needle, insert the needle at an inclined angle through the injection hole 54 of the push support part 5, press the needle against the inner wall of the blood receiving chamber 32, slowly inject the collected blood into the blood receiving chamber 32, and then remove the syringe to complete the blood injection operation.

[0068] 2. Based on the weight of the blood injected into the blood receiving chamber 32, inject balancing liquid into the blood receiving chamber 32 of another identical CGF separation and extraction device. Weigh the two CGF separation and extraction devices containing blood and balancing liquid. After the two CGF separation and extraction devices have the same weight, place the two CGF separation and extraction devices symmetrically in the hanging cup of the centrifuge and prepare for centrifugation separation.

[0069] Start the centrifuge, and the centrifuge will centrifuge the CGF separation and extraction device according to the preset centrifugation program.

[0070] The preset centrifugation program is set as follows: acceleration for 30 seconds; rotation at 2700 rpm / min for 2 minutes; rotation at 2400 rpm / min for 4 minutes; rotation at 2700 rpm / min for 4 minutes; rotation at 3000 rpm / min for 3 minutes; deceleration for 36 seconds.

[0071] 3. After centrifugation, carefully and steadily remove the CGF separation and extraction device containing blood, and rotate the sealing cover 2 counterclockwise one turn (360°) to separate the separation connection port 22 from the chamber channel 33, leaving a separation gap between the separation connection port 22 and the chamber channel 33.

[0072] 4. Install the pusher rod 71 of the pusher screw component 7 into the pusher guide hole 61 of the lower cover 6. Rotate the pusher screw component 7 to move it upward along the pusher guide hole 61 through the engagement of the internal thread and external thread of the lower cover assembly. After the pusher embedding protrusion 72 at the upper end of the pusher rod 71 and the pusher assembly groove 53 at the lower end of the pusher support 5 are embedded and fitted, continue to rotate the pusher rod 71 to push the separation piston 4 upward along the inner wall of the blood receiving chamber 32. The PPP layer of platelet-depleted plasma on the upper layer of the centrifuged separation liquid is separated from the separation gap and flows into the separation chamber 31 until the upper end face of the CGF layer is flush with the upper end of the chamber channel 33. Stop rotating the pusher screw component 7.

[0073] 5. Rotate the sealing cover 2 clockwise one full turn (360°) to align the separation connection port 22 with the chamber channel 33. The mating sealing protrusion 34 and the mating sealing groove 23 are then embedded together. At the same time, the front end of the extraction port 14 is embedded in the extraction port combination groove 36, so that the CGF collection cylinder 11 is connected to the blood containing chamber 32.

[0074] 6. Continue to rotate the lifting screw component 7 to push the separation piston 4 upward along the inner wall of the blood receiving chamber 32, so that CGF enters the CGF collection cylinder 11 through the chamber channel 33 and the separation connection port 22, until the white film layer is transferred into the CGF collection section 1, then stop rotating the lifting screw component 7.

[0075] 7. Hold the CGF collection part 1 and rotate the CGF collection cylinder 11 counterclockwise to remove the CGF collection part 1 from the CGF collection part receiving cavity 21, so that the CGF collection part 1 is separated from the sealing cover 2. Seal the CGF collection part 1 after CGF preparation or connect it to an injection needle for storage and future use, thus completing the separation and preparation of CGF from blood.

[0076] Furthermore, in step 2, the injection needle is preferably inserted into the injection hole 54 at a 45° angle to perform the blood injection operation.

[0077] Furthermore, the separation chamber 31 and blood containing chamber 32 of the preparation chamber 3, as well as the CGF collecting cylinder 11 of the CGF collecting section 1, are made of transparent material, allowing the operator to clearly observe the separation and preparation process within the preparation chamber 3 and the CGF collecting section 1 during the preparation operation, and accurately complete the CGF separation and preparation operation. The outer walls of the blood containing chamber 32 and the CGF collecting cylinder 11 are provided with volume scale markings, allowing the operator to accurately control the dosage extracted according to the corresponding requirements.

[0078] Second embodiment: This embodiment is similar to the first embodiment, except that in this embodiment, a sealing cap 25 is further installed at the upper opening of the CGF collection cavity 21.

[0079] After completing the CGF separation and preparation according to the operating procedure of the first embodiment, a sealing cap 25 is installed on the top cover of the CGF collection chamber 21 to keep the sealing cap 2 sealed to the inside of the preparation chamber 3. This allows for safer and more compliant handling of the used CGF separation and extraction device, facilitates recycling, and avoids accidental leakage of residual separation liquid during recycling, which could cause environmental pollution.

[0080] Third embodiment: This embodiment is similar to the first and second embodiments, except that in this embodiment, a PPP extraction part 8 and a chamber pressure regulating part 9 are provided on the upper end face of the sealing cover 2.

[0081] In this embodiment, the pressure regulating part 9 of the chamber is provided, wherein the pressure regulating and breathable membrane 92 is a waterproof and breathable membrane. During storage and separation sterilization operations, the pressure regulating and breathable membrane 92 allows the gas to maintain the communication between the inside and outside and maintains the effective sealing inside the separation chamber 31. The pressure regulating and breathable membrane 92, together with the pressure regulating hole 94 and pressure regulating hole 93 on the top cover, maintains the air pressure balance inside and outside the separation chamber 31.

[0082] In this embodiment, with the PPP extraction unit 8, after the CGF is prepared, if the operator needs to utilize the PPP stored in the separation chamber 31 after separation, the extraction needle can pass through the extraction hole sealing plug 82 and enter the separation chamber 31 through the PPP extraction hole 81, so that the bottom of the extraction needle is attached to the bottom of the separation chamber 31, and the PPP separated in the separation chamber 31 can be extracted by the extraction needle in a sealed state for later use.

[0083] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A CGF separation and extraction device, characterized in that, include: CGF collection part (1), sealing cover (2), preparation cavity (3), separation piston (4), lifting support part (5), lower cover (6), lifting spiral component (7); The preparation cavity (3) includes an upper separation chamber (31) and a lower blood containment chamber (32). The separation chamber (31) and the blood containment chamber (32) are connected through a chamber channel (33). The upper end of the chamber channel (33) is provided with an extraction port combination groove (36). The sealing cover (2) is installed on the separation chamber (31). The interior of the sealing cover (2) is provided with a CGF collection cavity (21). The bottom of the CGF collection cavity (21) is provided with a separation connection port (22). The separation connection port (22) is connected and combined with the chamber channel (33). The chamber channel (33) is provided with a mating sealing protrusion (34) above it, and a mating sealing groove (23) is provided below the separation connection port (22). The mating sealing protrusion (34) is embedded in the mating sealing groove (23). The CGF collection part (1) is installed inside the sealed cover (2). The CGF collection part (1) includes: CGF collection cylinder (11), separation extraction plug (12) and separation extraction operation rod (13). The separation extraction plug (12) is assembled at the lower end of the separation extraction operating rod (13), and the separation extraction plug (12) is slidably installed on the inner wall of the CGF collection cylinder (11); The lower end of the CGF collecting cylinder (11) is provided with an extraction port (14), the extraction port (14) extends into the interior of the separation connection port (22), and the front end of the extraction port (14) is inserted into the extraction port combination groove (36). The upper end of the inner wall of the CGF collection cavity (21) is provided with an internal thread for the cylinder assembly, and the outer wall of the CGF collection cylinder (11) is provided with an external thread for the cylinder assembly; the CGF collection part (1) is installed in the CGF collection cavity (21) through the external thread for the cylinder assembly and the internal thread for the cylinder assembly in a threaded connection. The upper end face of the sealed cover (2) is provided with a PPP extraction part (8) and a chamber pressure regulating part (9). The lifting support (5) is assembled and installed below the separating piston (4), and the bottom of the lifting support (5) is provided with an inclined injection hole (54). The separating piston (4) and the lifting support (5) are slidably installed in the blood containing chamber (32), and the lower cover (6) is installed at the lower opening of the blood containing chamber (32); The lifting screw component (7) is installed in the lifting guide hole (61) on the lower end face of the lower cover (6) by a threaded connection, and the upper end of the lifting screw component (7) is engaged with the lower end of the lifting support part (5).

2. The CGF separation and extraction apparatus according to claim 1, characterized in that, The upper end of the outer wall of the separation chamber (31) is provided with an external thread for the cover assembly, and the inner wall of the sealing cover (2) is provided with an internal thread for the cover assembly. The sealing cover (2) is rotatably installed above the separation chamber (31) through the threaded connection between the internal thread of the cover assembly and the external thread of the cover assembly. The combination of the separation chamber (31) and the sealing cover (2) is provided with a double-layer sealing structure. The piston has a sealing ring on its outer wall, and the sealing ring fits against the inner wall of the blood receiving chamber (32). The lower end of the outer wall of the blood containment chamber (32) is provided with an external thread for the lower cover assembly, and the inner wall of the lower cover (6) is provided with an internal thread for the lower cover assembly. The lower cover (6) is installed below the blood containment chamber (32) through the threaded connection between the internal thread of the lower cover assembly and the external thread of the lower cover assembly. The inner wall of the push guide hole (61) is provided with a push internal thread, and the outer wall of the push connecting rod (71) of the push screw component (7) is provided with a push external thread. The push screw component (7) is installed in the lower cover (6) through the threaded connection between the push external thread and the push internal thread. The upper end of the push link (71) is provided with a push embedding protrusion (72), and the push embedding protrusion (72) and the push combination groove (53) at the lower end of the push support (5) are embedded and connected.

3. The CGF separation and extraction apparatus according to claim 2, characterized in that, The double-layer sealing structure includes an upper cover sealing ring (35) installed below the external thread of the upper cover assembly and an upper cover assembly sealing ring (24) provided on the inner end face of the sealing upper cover (2). The upper cover sealing ring (35) is sealed to the inner wall of the sealing upper cover (2), and the upper cover assembly sealing ring (24) is sealed to the top of the upper opening of the separation chamber (31).

4. The CGF separation and extraction apparatus according to any one of claims 1-3, characterized in that, The lower end of the separating piston (4) is provided with a combination hole (41), and the interior of the combination hole (41) is provided with a combination limiting groove (42). The upper end of the lifting support (5) is provided with a piston assembly protrusion (51), and the top of the piston assembly protrusion (51) is provided with a combination limiting protrusion (52). The piston assembly protrusion (51) is assembled and installed inside the combination hole (41), and the combination limiting protrusion (52) is embedded in the combination limiting groove (42).

5. The CGF separation and extraction apparatus according to claim 4, characterized in that, The lower end of the lifting spiral component (7) is provided with a lifting grip part (73), and the outer wall of the lifting grip part (73) is provided with a lifting grip locking protrusion (74).

6. The CGF separation and extraction apparatus according to any one of claims 1-3, characterized in that, The PPP extraction part (8) includes a PPP extraction hole (81) and an extraction hole sealing plug (82) installed on the PPP extraction hole (81). The chamber pressure regulating part (9) includes a breathable membrane fixing rubber plug (91), a pressure regulating breathable membrane (92), a pressure regulating hole (93) and a pressure regulating mounting groove (95). The pressure regulating hole (93) is located in the middle of the pressure regulating mounting groove (95). The pressure regulating breathable membrane (92) is attached to the bottom of the pressure regulating mounting groove (95). The breathable membrane fixing rubber plug (91) is installed in the pressure regulating mounting groove (95). The breathable membrane fixing rubber plug (91) has a top cover pressure regulating hole (94) in the middle.

7. The CGF separation and extraction apparatus according to any one of claims 1-3, characterized in that, The separation chamber (31) and blood containment chamber (32) of the preparation cavity (3) and the CGF collection cylinder (11) of the CGF collection part (1) are made of transparent material. The outer walls of the blood containment chamber (32) and the CGF collection cylinder (11) are provided with capacity scales. An operation grip (15) is provided above the separation extraction operation rod (13), and the operation grip (15) has recesses (16) on both sides.

8. The CGF separation and extraction apparatus according to claim 1, characterized in that, A sealing cap (25) is installed at the upper opening of the CGF collection cavity (21).

9. A method using the CGF separation and extraction apparatus according to any one of claims 1-8, characterized in that, Includes the following steps: Step 1: Inject the blood collected as needed into the blood containment chamber (32) through the injection hole (54) at the bottom of the lifting support (5); Step 2: Select equal weights of CGF separation and extraction devices for balancing and place them simultaneously into a centrifuge. Use the dedicated differential centrifugation program prepared for CGF for differential centrifugation. The dedicated differential centrifugation program is as follows: acceleration for 30 seconds; centrifugal acceleration of 692g for 2 minutes; centrifugal acceleration of 547g for 4 minutes; centrifugal acceleration of 692g for 4 minutes; centrifugal acceleration of 855g for 3 minutes; deceleration for 36 seconds. Step 3: After centrifugation, screw the sealing cap (2) up to separate the separation connection port (22) from the chamber channel (33) and leave a separation gap; Step 4: By turning the lifting screw component (7) installed on the lower cover (6), the lifting connecting rod (71) and the separation piston (4) are driven to move upward, and the upper layer PPP of the separated blood is transferred to the separation chamber (31). Step 5: Twist the sealing cap (2) to lower it, so that the separation connection port (22) is fully connected with the chamber channel (33), and the CGF collection cylinder (11) is connected to the blood containment chamber (32); Step 6: Continue to move the push rod (71) and the separation piston (4) upward by turning the push screw component (7) installed on the lower cover (6) to completely transfer the target product CGF (concentrated growth factor) into the CGF collection section (1); Step 7: Hold and twist the CGF collection cylinder (11) upwards to unscrew the CGF collection part (1) from the CGF collection part receiving cavity (21), store the CGF collection part (1) containing the target product CGF for later use, and complete the extraction and collection.

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