Method for rapidly germinating seeds of sinosenecio crassifolius

Abstract

The application provides a kind of fast germination method of chuanmingshen seed, comprising the following steps: in the bottom of first container, lay a layer of white defatted cotton, wet defatted cotton with Tris-hydrochloride solution;Chuanmingshen seed is laid on defatted cotton with first density, and the first container is sealed, and incubation treatment is carried out in dark condition;Wherein, first density meets that adjacent chuanmingshen seed does not overlap each other;During incubation treatment, Tris-hydrochloride solution is supplemented into the first container, and the liquid level in the first container is kept flush with the top of chuanmingshen seed;The treated chuanmingshen seed is placed in second container for germination culture.The above-mentioned method solves the problems of long germination time and low seed germination rate caused by thick seed coat and low permeability of chuanmingshen seed, shortens the germination time of chuanmingshen, and provides a basis for evaluating the seed vigor and germination capacity of chuanmingshen in a short period.

Description

Technical Field

[0001] This invention belongs to the field of forest tree propagation technology, specifically relating to a method for rapid germination of Sichuan ginseng seeds. Background Technology

[0002] *Codonopsis pilosula*, belonging to the genus *Codonopsis* in the family Apiaceae, is a traditional Chinese medicinal herb. Its dried underground root, commonly known as *Ming Sha Shen* or *Ming Shen*, is highly valued for its stomach-soothing, fluid-generating, lung-moistening, and phlegm-resolving properties. As a herbaceous medicinal plant, and because its medicinal part is the underground tuber, harvesting mature *Codonopsis pilosula* requires the entire plant to be destructively dug up. Therefore, large-scale cultivation of *Codonopsis pilosula* currently relies on seed propagation. However, seed propagation of *Codonopsis pilosula* presents significant challenges. The seeds are small and short-lived; most seeds from the previous year lose their germination ability, and maturity is uneven. Furthermore, the seed coat is leathery, thick, and has low permeability, resulting in low germination rates and prolonged germination times.

[0003] Currently, most methods utilize corrosive agents such as concentrated sulfuric acid, hydrogen peroxide, and quicklime, or mechanical damage methods like grinding, to break down the seed coat, aiming to shorten germination time and increase germination rate. However, the concentrated sulfuric acid and quicklime used in these methods are hazardous chemicals. Furthermore, the small size and uneven maturity of *Codonopsis pilosula* seeds make it difficult to control the degree of chemical corrosion and physical damage. Insufficient or excessive treatment can lead to seed inactivation, resulting in a still low germination rate. While KMnO4 is also used to treat *Codonopsis pilosula* seeds, the germination time remains long, preventing growers from quickly assessing seed viability and thus missing the optimal planting period. This significantly limits the development of large-scale artificial propagation of *Codonopsis pilosula*.

[0004] Therefore, how to provide a method for rapidly germinating Sichuan ginseng seeds that can shorten germination time and improve seed germination rate is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] This invention provides a method for rapid germination of Sichuan ginseng seeds. By incubating the Sichuan ginseng seeds, the method solves the problems of long germination time and low germination rate caused by the thick seed coat and low permeability of Sichuan ginseng seeds, thus shortening the germination time and providing a basis for evaluating the vigor and germination ability of Sichuan ginseng seeds in a short period of time.

[0006] This invention provides a method for rapid germination of Sichuan ginseng seeds, comprising the following steps:

[0007] Line the bottom of the first container with a layer of white absorbent cotton and moisten the cotton with Tris-hydrochloride solution;

[0008] The seeds of Codonopsis pilosula were spread evenly on absorbent cotton at a first density, and the first container was sealed and incubated in the dark. The first density was such that adjacent seeds of Codonopsis pilosula did not overlap. During the incubation process, Tris-hydrochloride solution was added to the first container, and the liquid level in the first container was kept level with the top of the seeds of Codonopsis pilosula.

[0009] The treated Sichuan ginseng seeds were placed in a second container for germination culture.

[0010] The method described above, wherein the pH of the Tris-hydrochloride solution is 4.2–6.0; and / or,

[0011] The Tris-hydrochloride solution contains 4-8% Tris-hydrochloride by mass.

[0012] In the method described above, the incubation treatment is carried out at a temperature of 35–40°C for a duration of 24–72 hours.

[0013] The method described above, before spreading the Sichuan ginseng seeds on absorbent cotton at a first density, further includes pre-treatment of the Sichuan ginseng seeds. The pre-treatment includes the following steps: placing the Sichuan ginseng seeds, laundry detergent, Tween 20, and water in a sealed container, shaking and washing thoroughly for 4-6 minutes, then rinsing the Sichuan ginseng seeds with running water for 15-25 minutes; subsequently soaking the Sichuan ginseng seeds in sodium hypochlorite solution for 4-6 minutes, and then rinsing with water.

[0014] The effective chlorine content in the sodium hypochlorite solution is 0.3-0.6%; the mass ratio of Sichuan ginseng seeds to laundry detergent is (90-110):(4.5-5.5g); and the mass-volume ratio of Sichuan ginseng seeds, Tween 20, and water is (90-110g):(450-550μL):(450-550mL).

[0015] The method described above further includes, after incubation, soaking the incubated Codonopsis pilosula seeds in a solution, wherein the solution for soaking is selected from at least one of potassium permanganate solution and gibberellin solution.

[0016] The method described above also includes soaking the incubated ginseng seeds in water and subjecting them to ultrasonic treatment under the action of ultrasound.

[0017] The method described above, wherein the mass concentration of potassium permanganate in the potassium permanganate solution is 1–1.4%; and / or,

[0018] The concentration of gibberellin in the gibberellin solution is 30–40 mg / L; and / or,

[0019] The soaking treatment is performed at a temperature of 4°C for 12–25 hours.

[0020] In the method described above, the temperature of the ultrasonic treatment is not higher than 25°C, the time is 10 to 20 minutes, and the frequency of the ultrasonic wave is 30 to 50 kHz.

[0021] The method described above, wherein the solution soaking treatment of the incubated ginseng seeds includes the following steps:

[0022] The incubated ginseng seeds were soaked in potassium permanganate solution, water and gibberellin solution in sequence. The soaking of ginseng seeds in water was assisted by ultrasound.

[0023] The method described above, wherein placing the treated Sichuan ginseng seeds in a second container for germination cultivation includes the following steps:

[0024] Place a layer of defatted cotton at the bottom of the second container, spread the soaked Sichuan ginseng seeds on the defatted cotton, and cover the side of the Sichuan ginseng seeds away from the defatted cotton with a layer of filter paper.

[0025] Add distilled water to the second container to ensure that the liquid level in the second container is level with the bottom of the Sichuan ginseng seeds, and keep the filter paper moist; the temperature for germination culture is 18-22℃, and the light and dark are alternated for 12 hours a day, with a light intensity of 1000-1500 Lx.

[0026] The implementation of this invention has at least the following beneficial effects:

[0027] The method for rapid germination of Sichuan ginseng seeds provided by this invention, through incubation treatment of Sichuan ginseng seeds, not only solves the problem of long germination time caused by the thick seed coat and low permeability of Sichuan ginseng seeds, but also helps to improve the penetration effect of subsequent solution soaking treatment. This shortens the germination initiation date of Sichuan ginseng seeds to within 21 days, facilitating the evaluation of seed vigor and germination ability within the optimal sowing period of Sichuan ginseng, and providing guidance for timely replanting. Secondly, it can also improve the seed germination rate, specifically increasing the germination rate of Sichuan ginseng seeds to over 38% within 30 days, while ensuring that the germination uniformity of Sichuan ginseng seeds in the same batch is basically consistent. This helps to save seed usage in actual production sowing, improve seedbed utilization, and facilitate subsequent planting and maintenance management. Furthermore, the method provided by this invention is simple, convenient to operate, and does not produce toxic or harmful waste, enabling large-scale seed processing in actual production. Attached Figure Description

[0028] Figure 1 This is the germination status of the Sichuan ginseng seeds 20 days after the germination process in Example 1;

[0029] Figure 2 This describes the growth of *Gynostemma pentaphyllum* seeds in Example 1 after germination and transplanting to a seedbed 60 days later.

[0030] Figure 3 This is the germination status of the Sichuan ginseng seeds 20 days after the germination process in Example 3. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions in the embodiments of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0032] In a first aspect, the present invention provides a method for rapid germination of Codonopsis pilosula seeds, comprising the following steps: laying a layer of white absorbent cotton at the bottom of a first container and moistening the absorbent cotton with Tris-hydrochloride solution; spreading Codonopsis pilosula seeds evenly on the absorbent cotton at a first density, sealing the first container, and incubating in the dark; wherein the first density ensures that adjacent Codonopsis pilosula seeds do not overlap; during the incubation process, adding Tris-hydrochloride solution to the first container, ensuring that the liquid level in the first container is level with the top of the Codonopsis pilosula seeds; and placing the treated Codonopsis pilosula seeds in a second container for germination cultivation.

[0033] The seeds of *Gynostemma pentaphyllum* in this invention are used as the target for germination. The purpose of germination is to induce the seeds to sprout, that is, to begin germination. When it is necessary to propagate, cultivate, or apply *Gynostemma pentaphyllum*, the germinated seeds can be directly transplanted to cultivate large-diameter timber.

[0034] A layer of white absorbent cotton was placed at the bottom of the first container to form a layer of white absorbent cotton with a certain thickness, making it easy to observe the moisture level of the absorbent cotton and the liquid level in the first container. The absorbent cotton was moistened with Tris-hydrochloride solution to ensure that the absorbent cotton could absorb enough Tris-hydrochloride solution, which would be beneficial for subsequent incubation treatment.

[0035] The seeds of *Codonopsis pilosula* were laid flat on absorbent cotton at a density of 1, with the seeds spaced apart and not overlapping. The first container was then sealed to create a near-sealed space. Sealing with plastic wrap was recommended. This sealing provides the necessary humidity for seed germination, prevents excessive water evaporation leading to frequent watering, and limits the stress from external factors such as non-target species, pests, and diseases.

[0036] During the incubation process, Tris-hydrochloride solution is added to the first container, ensuring that the liquid level in the first container is flush with the top of the Sichuan ginseng seeds. This means that the Tris-hydrochloride solution in the first container not only soaks the absorbent cotton but also has excess solution submerging the Sichuan ginseng seeds. This incubation process softens the seed coat, facilitating germination and emergence, and also breaks down the impermeability of the seed coat, improving the water and air permeability of the seeds. This enhances the penetration effect of subsequent solution soaking, maximizing the benefits of solution soaking.

[0037] After incubation, the Sichuan ginseng seeds are soaked in a solution, which helps to further promote germination, shorten germination time, and improve germination rate. Finally, the soaked Sichuan ginseng seeds are placed in a second container for germination cultivation. Due to the combination of incubation and solution soaking treatments, the germination uniformity of Sichuan ginseng seeds in the same batch can be guaranteed.

[0038] Furthermore, in actual production, seed vigor and germination ability are evaluated based on germination status, and reseeding is carried out to meet density requirements. Specifically, during the germination culture process, seed germination is observed daily. If seeds fail to germinate, germinate unevenly, or do not meet density requirements, additional compensation seeds are promptly added to the areas with abnormal seeds to ensure that seeds in those areas germinate as much as possible. Because the germination time of Sichuan ginseng seeds is shortened, it is beneficial to evaluate seed vigor and germination ability within the optimal sowing period, providing guidance for timely reseeding. The optimal sowing period refers to the 7 days before and after the Autumnal Equinox, and sowing can be carried out on any day within this optimal sowing period.

[0039] The first and second containers can be conventional petri dishes, seed germination boxes, etc., as long as they are of suitable size, have good light transmission, and can maintain air humidity. Preferably, the first and second containers are transparent, which facilitates observation and provides light to maintain the necessary photosynthesis of Sichuan ginseng seeds to prevent them from dying.

[0040] This invention does not limit the specific parameters of the Tris-hydrochloride solution, which can be adjusted according to actual needs. In some embodiments, the pH of the Tris-hydrochloride solution is 4.2 to 6.0; and / or, the mass content of Tris-hydrochloride in the Tris-hydrochloride solution is 4% to 8%, for example, a range of 4%, 5%, 6%, 7%, 8%, or any combination thereof.

[0041] The present invention does not limit the operating conditions of the incubation treatment, and can be adjusted according to the actual situation. For example, in some embodiments, the incubation temperature is 35-40°C, such as 35°C, 36°C, 37°C, 38°C, 39°C, 40°C or any combination thereof, and the time is 24-72h, such as 24h, 36h, 72h or any combination thereof.

[0042] Before implementing the incubation treatment, the process also includes seed selection. In this invention, healthy, plump, pure, and uniformly sized Sichuan ginseng seeds can be selected as research objects.

[0043] The seed coat of *Codonopsis pilosula* has many grooves. To prevent mold and rot during germination, the seeds need to be cleaned and disinfected. The cleaning and disinfection solution should be of appropriate concentration, non-toxic to the seeds, and capable of killing fungi on the seed surface. For example, in some embodiments, before spreading the Sichuan ginseng seeds on absorbent cotton at a first density, the Sichuan ginseng seeds are pretreated. The pretreatment includes the following steps: placing the Sichuan ginseng seeds, laundry detergent, Tween 20 and water in a sealed container, shaking and washing thoroughly for 4-6 minutes, and then rinsing the Sichuan ginseng seeds with running water for 15-25 minutes; subsequently soaking the Sichuan ginseng seeds in sodium hypochlorite solution for 4-6 minutes, and then rinsing with water; wherein the effective chlorine content in the sodium hypochlorite solution is 0.3-0.6%; the mass ratio of Sichuan ginseng seeds to laundry detergent is (90-110):(4.5-5.5g), and the mass-volume ratio of Sichuan ginseng seeds, Tween 20 and water is (90-110g):(450-550μL):(450-550mL).

[0044] Specifically, the pretreatment process is as follows: Place 90–110 g of seeds, 4.5–5.5 g of laundry detergent, 450–550 μL of Tween 20, and 450–550 mL of water into a sealed container and shake thoroughly for 4–6 minutes. After shaking and washing, rinse with running water for 15–25 minutes, then soak in a sodium hypochlorite solution with an effective chlorine content of 0.3–0.6% for 4–6 minutes. Finally, rinse with ultrapure water to remove any residual sodium hypochlorite. Here, effective chlorine content refers to oxidizing capacity. When the effective chlorine content in the sodium hypochlorite solution is 0.5%, it means that the oxidizing capacity of 100 g of sodium hypochlorite solution when fully reacted is equivalent to the oxidizing capacity of 0.5 g of chlorine.

[0045] The incubated *Codonopsis pilosula* seeds are subjected to a solution soaking treatment. In some embodiments, this soaking treatment is further performed after incubation. The solution used for soaking is selected from at least one of potassium permanganate solution and gibberellin solution. That is, potassium permanganate solution, gibberellin solution, or a combination of both can be used for the soaking treatment. When potassium permanganate solution and / or gibberellin solution are used for soaking the incubated *Codonopsis pilosula* seeds, a certain degree of sterilization and disinfection is achieved, preventing the formation of mold and mycelium, reducing the rate of seed mold and rot, and ensuring normal seed growth.

[0046] Furthermore, the mass concentration of potassium permanganate in the potassium permanganate solution is 1–1.4%; and / or the concentration of gibberellin in the gibberellin solution is 30–40 mg / L; and / or the soaking treatment temperature is 4°C and the time is 12–25 h.

[0047] During the soaking process, the ratio of the number of Sichuan ginseng seeds to the volume of the solution should be: 100 Sichuan ginseng seeds : 100 mL solution.

[0048] In some embodiments, the method further includes soaking the incubated Codonopsis pilosula seeds in water and then subjecting them to ultrasonic treatment. Ultrasonic treatment can further overcome the defects of Codonopsis pilosula seeds, such as thick seed coats and low permeability, thereby shortening germination time and increasing germination rate.

[0049] Furthermore, the ultrasonic treatment temperature is no higher than 25℃, the time is 10-20 min, and the ultrasonic frequency is 30-50 kHz.

[0050] During ultrasonic treatment, the ratio of the number of Sichuan ginseng seeds to the volume of water should be: 100 Sichuan ginseng seeds: 500 mL of water. Specifically, the seeds are soaked in pure water at a ratio of 100 Sichuan ginseng seeds / 500 mL and then ultrasonically treated. Ice is added during the process to ensure that the liquid temperature does not exceed 25°C.

[0051] In a preferred embodiment, the solution soaking treatment of the incubated *Codonopsis pilosula* seeds includes the following steps: the incubated *Codonopsis pilosula* seeds are soaked sequentially in potassium permanganate solution, water, and gibberellin solution, wherein the soaking of *Codonopsis pilosula* seeds in water is assisted by ultrasound. Specifically, the *Codonopsis pilosula* seeds are first soaked in potassium permanganate solution at a ratio of 100 seeds / 100 mL; then, the *Codonopsis pilosula* seeds are soaked in 500 mL of pure water at a ratio of 100 seeds / 500 mL and treated in an ultrasonic machine; finally, the *Codonopsis pilosula* seeds are soaked in gibberellin solution at a ratio of 100 seeds / 100 mL.

[0052] In some embodiments, placing the soaked and treated Sichuan ginseng seeds in a second container for germination cultivation includes the following steps: laying a layer of defatted cotton at the bottom of the second container, spreading the soaked and treated Sichuan ginseng seeds flat on the defatted cotton, and covering the side of the Sichuan ginseng seeds away from the defatted cotton with a layer of filter paper; adding distilled water to the second container to ensure that the liquid level in the second container is level with the bottom of the Sichuan ginseng seeds, and ensuring that the filter paper is moist; the germination cultivation temperature is 18-22℃, with alternating 12h light and 12h darkness per day, and the light intensity is 1000-1500 Lx.

[0053] The present invention will be further described below through specific embodiments and comparative examples. Unless otherwise specified, the reagents, materials and instruments used below are all conventional reagents, materials and instruments, all of which are commercially available, and the reagents and materials involved can also be synthesized by conventional synthetic methods.

[0054] Example 1

[0055] This embodiment describes a method for rapid germination of Sichuan ginseng seeds, including the following steps:

[0056] S1. Pretreatment: Place 100g of Sichuan ginseng seeds, 5g of laundry detergent, 500μL of Tween 20 and 500mL of water into a sealed container, shake and wash thoroughly for 5 minutes. After shaking and washing, rinse with running water for 20 minutes. Then soak the Sichuan ginseng seeds in a sodium hypochlorite solution with an effective chlorine content of 0.5% for 5 minutes. After that, rinse with ultrapure water to remove the residual liquid.

[0057] S2. Incubation treatment: Place a layer of white defatted cotton at the bottom of the incubation container. After wetting the defatted cotton with a 5% Tris-hydrochloride solution at pH 5.5, spread the Sichuan ginseng seeds (after step S1) in a single layer on the defatted cotton. Seal the incubation container with plastic wrap and place it in the dark at 38°C for 48 hours. During this period, observe and replenish the Tris-hydrochloride solution to ensure that the liquid level in the incubation container is level with the top of the seeds.

[0058] S3. Potassium permanganate solution soaking treatment: Use a 1.2% potassium permanganate solution to soak the Sichuan ginseng seeds after step S2 at a ratio of 100 seeds / 100mL for 14h at 4℃.

[0059] S4. Ultrasonic treatment: Soak the Sichuan ginseng seeds after step S3 in pure water at a ratio of 100 seeds / 500mL, and treat them in an ultrasonic machine at a frequency of 40kHz for 15min. During this period, add ice to ensure that the liquid temperature in the ultrasonic machine does not exceed 25℃.

[0060] S5. Gibberellin solution soaking treatment: Use 35 mg / L gibberellin (GA3) solution to soak the Sichuan ginseng seeds after step S4 for 20 h at a ratio of 100 seeds / 100 mL;

[0061] S6. Germination: Place defatted cotton at the bottom of the petri dish, place the Sichuan ginseng seeds after step S5 in the petri dish, cover with a layer of filter paper, and pour in distilled water. The standard is that the water surface is in contact with the bottom of the seed after pouring. The cultivation environment is at room temperature of 20℃, with 12 hours of light and 12 hours of darkness alternating every day, and the light intensity is 1200 Lx.

[0062] Example 2

[0063] The germination method is basically the same as that in Example 1, except that step S3 is omitted and the seeds of Codonopsis pilosula after step S2 are directly used in step S4 of the example, while other conditions remain unchanged.

[0064] Example 3

[0065] The germination method is basically the same as that in Example 1, except that step S4 is omitted and the seeds of Codonopsis pilosula after step S3 are directly used in step S5 of the example, while other conditions remain unchanged.

[0066] Example 4

[0067] The germination method is basically the same as that in Example 1, except that step S5 is omitted and the seeds of Codonopsis pilosula after step S4 are directly used in step S6 of the example, while other conditions remain unchanged.

[0068] Example 5

[0069] The germination method is basically the same as that in Example 1, except that steps S3, S4 and S5 are omitted, and the seeds of Sichuan ginseng after step S2 are directly used in step S6 of the example, while other conditions remain unchanged.

[0070] Comparative Example 1

[0071] The germination method is basically the same as that in Example 1, except that step S2 is omitted and the seeds of Codonopsis pilosula after step S1 are directly used in step S3 of the example, while other conditions remain unchanged.

[0072] Comparative Example 2

[0073] The germination method is basically the same as that in Example 1, except that steps S2 and S3 are omitted, and the seeds of Codonopsis pilosula after step S1 are directly used in step S4 of the example; other conditions remain unchanged.

[0074] Comparative Example 3

[0075] The germination method is basically the same as that in Example 1, except that steps S2 and S4 are omitted, and the seeds of Codonopsis pilosula after step S1 are directly used in step S3 of the example, and the seeds of Codonopsis pilosula after step S3 are used in step S5 of the example, with other conditions remaining unchanged.

[0076] Comparative Example 4

[0077] The germination method is basically the same as that in Example 1, except that steps S2 and S5 are omitted, and the seeds of Codonopsis pilosula after step S1 are directly used in step S3 of the example, and the seeds of Codonopsis pilosula after step S4 are used in step S6 of the example, while other conditions remain unchanged.

[0078] Comparative Example 5

[0079] The germination method is basically the same as that in Example 1, except that steps S2, S3, S4 and S5 are omitted, and the seeds of Sichuan ginseng after step S1 are directly used in step S6 of the example.

[0080] Test case

[0081] Using 80 seeds of Sichuan ginseng as the research object, the above-mentioned methods for germination were adopted and compared. According to the germination status and germination time, the germination time corresponding to the earliest germination of the seeds was recorded, and the number of seeds that germinated within 30 days was recorded. The germination rate in 30 days was calculated as: number of seeds that germinated within 30 days / total number of Sichuan ginseng seeds * 100. The results are shown in Table 1.

[0082] Table 1

[0083] Example 1 14 49.32％ Example 2 16 47.14％ Example 3 21 42.67％ Example 4 20 44.00％ Example 5 16 38.64％ Comparative Example 1 23 14.86％ Comparative Example 2 26 15.79％ Comparative Example 3 22 14.67％ Comparative Example 4 24 13.33％ Comparative Example 5 45 8.33％

[0084] Figure 1 The image shows the germination of *Gynostemma pentaphyllum* seeds 20 days after the germination process in Example 1. Figure 2 The growth of the Sichuan ginseng seeds in Example 1 after germination and transplanting to the seedbed for 60 days is shown. Figure 3 This refers to the germination status of *Gynostemma pentaphyllum* seeds 20 days after the germination process in Example 3. Figure 1and Figure 3 It can be seen that, compared with Example 2, there is moldy mycelium in Example 3.

[0085] As shown in Table 1, the present invention, through incubation treatment, can significantly shorten the germination time and increase the germination rate of *Codonopsis pilosula* seeds. In particular, the fastest germination start date for *Codonopsis pilosula* seeds in Example 1 is 14 days, and in Example 2 it is 16 days, significantly shortening the germination time. Furthermore, Example 1 increases the germination rate to 49.32%, greatly overcoming the problems of low germination rate and uneven germination uniformity in *Codonopsis pilosula* seeds. Comparing Examples 1-5, Example 5 shows that some seeds became moldy during the germination process, resulting in a lower overall germination rate. Example 3, compared to Example 2, shows mold mycelium formation, which may subsequently affect the normal growth of the seeds. Therefore, adding steps such as soaking in potassium permanganate solution, ultrasonic treatment, and gibberellin solution treatment helps to further shorten the germination time and increase the germination rate of *Codonopsis pilosula* seeds.

[0086] The preferred embodiments and experimental verifications of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the protection scope of the present invention.

Claims

1. A method for rapid germination of Sichuan ginseng seeds, characterized in that, Includes the following steps: Line the bottom of the first container with a layer of white absorbent cotton and moisten the cotton with Tris-hydrochloride solution; The seeds of Codonopsis pilosula were spread evenly on absorbent cotton at a first density, and the first container was sealed and incubated in the dark. The first density was such that adjacent seeds of Codonopsis pilosula did not overlap. During the incubation process, Tris-hydrochloride solution was added to the first container, and the liquid level in the first container was kept level with the top of the seeds of Codonopsis pilosula. The treated Sichuan ginseng seeds were placed in a second container for germination culture. The incubation treatment is carried out at a temperature of 35~40℃ for 24~72h. The incubation process includes soaking the incubated Sichuan ginseng seeds in a solution, wherein the solution is selected from at least one of potassium permanganate solution and gibberellin solution; the mass concentration of potassium permanganate in the potassium permanganate solution is 1-1.4%. The concentration of gibberellin in the gibberellin solution is 30~40 mg / L; The soaking treatment is performed at a temperature of 4°C for 12-25 hours. This also includes soaking the incubated ginseng seeds in water and then subjecting them to ultrasonic treatment under the action of ultrasound. The ultrasonic treatment temperature is no higher than 25°C, the time is 10~20 min, and the ultrasonic frequency is 30~50 kHz. The pH of the Tris-hydrochloride solution is 4.2~6.0; The Tris-hydrochloride solution contains 4-8% Tris-hydrochloride by mass.

2. The method according to claim 1, characterized in that, Before spreading the Sichuan ginseng seeds evenly on the absorbent cotton at the first density, the Sichuan ginseng seeds are also pretreated. The pretreatment includes the following steps: placing the Sichuan ginseng seeds, laundry detergent, Tween 20 and water in a sealed container, shaking and washing thoroughly for 4-6 minutes, then rinsing the Sichuan ginseng seeds with running water for 15-25 minutes; then soaking the Sichuan ginseng seeds in sodium hypochlorite solution for 4-6 minutes, and then rinsing with water. The effective chlorine content in the sodium hypochlorite solution is 0.3-0.6%; the mass ratio of Sichuan ginseng seeds to laundry detergent is (90-110g): (4.5-5.5g); and the mass-volume ratio of Sichuan ginseng seeds, Tween 20, and water is (90-110g): (450-550μL): (450-550mL).

3. The method according to claim 1, characterized in that, The solution soaking treatment of the incubated ginseng seeds includes the following steps: The incubated ginseng seeds were soaked in potassium permanganate solution, water and gibberellin solution in sequence. The soaking of ginseng seeds in water was assisted by ultrasound.

4. The method according to claim 1, characterized in that, The treated Sichuan ginseng seeds were placed in a second container for germination and cultivation, including the following steps: Place a layer of defatted cotton at the bottom of the second container, spread the soaked Sichuan ginseng seeds on the defatted cotton, and cover the side of the Sichuan ginseng seeds away from the defatted cotton with a layer of filter paper. Add distilled water to the second container to ensure that the liquid level in the second container is level with the bottom of the Sichuan ginseng seeds, and keep the filter paper moist; The temperature for germination culture is 18~22℃, with alternating 12h light and 12h darkness per day, and a light intensity of 1000~1500Lx.

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