Multifunctional bionic oral cavity repairing support and manufacturing method thereof

Abstract

The application discloses a multifunctional bionic oral cavity repairing support and a manufacturing method thereof, wherein the multifunctional bionic oral cavity repairing support is provided with a convex part and a concave part, so as to simulate the combined structure of natural periodontal connective tissue and alveolar bone, ensure that the space of osteoblasts invaded by fibroblasts is limited, and maximize the retention of the natural growth space of osteogenic fibers, so that the periodontal tissue and the alveolar bone are more closely and stably combined. In addition, the multifunctional bionic oral cavity repairing support comprises a three-layer structure, the loose layer is in contact with soft tissue and can effectively repair the soft tissue, the dense layer effectively blocks fibroblasts, and the bone repairing layer is more conducive to the repair of the alveolar bone. In addition, the biological membrane is taken from animals, has low cost, and has no complicated collagen extraction process, and collagen fibers are extracted, compared with a pure collagen oral cavity repairing support, the mechanical property of the collagen fibers is greatly improved, and the collagen fibers are convenient to use in a surgical process.

Description

Technical Field

[0001] This invention relates to the technical field of dental restoration equipment, and in particular to a multifunctional bionic dental restoration framework and its manufacturing method. Background Technology

[0002] In recent years, dental implants have become increasingly widely used. The key to successful implant restoration is ensuring sufficient alveolar bone reserve or sufficient alveolar bone augmentation in the tooth area. For patients with severe periodontitis, after tooth extraction, the gums and other soft tissues will atrophy and the alveolar ridge will be absorbed over time. This will affect the shape of the gums and the fullness of the alveolar bone in the extraction area, resulting in alveolar bone loss, reduced bone width and height, etc., which will affect the effect of subsequent denture restoration or implant placement and is not conducive to the restoration of missing teeth in the later stage.

[0003] Different cell types have varying characteristics, growth rates, and migration speeds. For example, bone cells typically grow slowly, while fibroblasts in periodontal soft tissues grow rapidly. Therefore, during bone repair, if these two cell types are not effectively isolated, osteoblasts and fibroblasts can easily encroach on each other's space and compete for nutrients, leading to competitive inhibition. This results in bone cells lacking sufficient space and adequate nutrition to maintain normal bone tissue growth. To address this, dental prosthetic frameworks have been developed. These frameworks are surgically placed between the oral soft tissues and bone defects to create a biological barrier, thereby creating a relatively closed bone regeneration environment. This selectively blocks faster-migrating fibroblasts and epithelial cells from entering the bone defect area, while not hindering natural wound healing.

[0004] However, existing dental prosthetic frameworks only focus on the occupation of osteogenic space by fibroblasts, making them act as a physical barrier to prevent the growth of gingival connective tissue and epithelium into the defect, providing and maintaining the corresponding osteogenic space, and creating a relatively closed and favorable space for the regeneration of periodontal tissues and the repair of peri-implant bone defects, but neglect the functional role of fibroblasts in the natural formation of alveolar bone. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a multifunctional bionic oral restoration framework.

[0006] To achieve the above objectives, the technical solution provided by this invention is as follows:

[0007] A multifunctional bionic oral prosthetic support includes a loose layer that contacts soft tissue, a dense layer that provides physical shielding and antibacterial function, and a bone repair layer that guides bone tissue regeneration. All three layers have the same shape, are wavy, and have convex and concave portions.

[0008] The dense layer is located between the loose layer and the bone repair layer, and is closely attached to both the loose layer and the bone repair layer.

[0009] In this technical solution, the multifunctional bionic oral restoration framework has convex and concave parts, thereby simulating the natural combination structure of periodontal connective tissue and alveolar bone. This ensures that while restricting fibroblasts from encroaching on osteoblast space, it can maximize the preservation of the natural growth space of osteoblast fibers, making the periodontal tissue and alveolar bone more tightly and stably combined.

[0010] In addition, the multifunctional bionic oral restoration framework has a three-layer structure. The loose layer is in contact with soft tissue and can effectively repair soft tissue; the dense layer effectively blocks fibroblasts; and the bone repair layer is more conducive to the repair of alveolar bone.

[0011] Furthermore, the porous layer is made of a mixture of collagen fibers and carboxymethyl chitosan;

[0012] The dense layer is made of carboxymethyl chitosan material;

[0013] The bone repair layer is made of a mixture of carboxymethyl chitosan, hydroxyapatite, and collagen fibers.

[0014] In this technical solution, the loose layer, dense layer, and bone repair layer all contain carboxymethyl chitosan, which can promote wound healing, that is, facilitate fibrous hyperplasia and new blood vessel formation.

[0015] Adding hydroxyapatite to the bone repair layer helps guide bone tissue regeneration.

[0016] To achieve the above objectives, the present invention further provides a method for fabricating a multifunctional bionic dental prosthetic framework, used to fabricate the aforementioned multifunctional bionic dental prosthetic framework, the method comprising:

[0017] Preparation of collagen fiber slurry;

[0018] Preparation of carboxymethyl chitosan slurry;

[0019] Carboxymethyl chitosan slurry and collagen fiber slurry are mixed to prepare carboxymethyl chitosan-collagen fiber mixed slurry;

[0020] Carboxymethyl chitosan slurry, collagen fiber slurry, and hydroxyapatite are mixed to prepare a carboxymethyl chitosan slurry-collagen fiber slurry-hydroxyapatite mixed slurry;

[0021] Carboxymethyl chitosan slurry, carboxymethyl chitosan-collagen fiber mixed slurry, and carboxymethyl chitosan-collagen fiber-hydroxyapatite mixed slurry were respectively installed in the corresponding nozzles of the 3D printer for printing, thereby obtaining a three-layer oral restoration framework.

[0022] Strengthen the connections between the three layers of the dental prosthesis framework;

[0023] The reinforced dental prosthetic framework is then processed to obtain a multifunctional bionic dental prosthetic framework.

[0024] Further, the preparation of collagen fiber slurry includes:

[0025] Animal viscera are obtained and processed to obtain animal-derived biofilms;

[0026] Animal-derived biofilms are cut into strips of a predetermined length using sterile scissors;

[0027] Prepare a 0.1-0.5% benzalkonium chloride solution. Take 1 volume of a long strip of animal-derived biofilm and add it to 10 times the volume of benzalkonium chloride solution for disinfection. Soak for 10-30 minutes. After that, take it out and soak and wash it with sterile water 3-5 times, 10-30 minutes each time.

[0028] Prepare a NaOH solution with a concentration of 1-4 mol / L. Take 1 volume of sterilized animal biofilm and add it to 10 times the volume of NaOH solution for virus inactivation. Soak for 1-4 hours. After completion, take it out and soak and wash it with sterile water 3-5 times, each time for 10-30 minutes.

[0029] Prepare a 0.5-2% sodium deoxycholate solution. Take 1 volume of animal-derived biofilm that has been inactivated by virus and add it to 10 times the volume of sodium deoxycholate solution for degreasing. Soak for 24-48 hours. After completion, take it out and soak and wash it with sterile water 3-5 times, 30 minutes each time. Repeat the degreasing step twice.

[0030] Prepare an X-100 Triton solution with a concentration of 0.5-1.5%. Take 1 volume of defatted animal biomembrane and add it to 10 volumes of X-100 Triton solution for decellularization. Soak for 24-48 hours. After completion, remove the biomembrane and soak and wash it with sterile water 3-5 times, 30 minutes each time. Repeat the decellularization step twice to obtain an antigen-free animal biomembrane layer.

[0031] The deantigenated animal-derived biofilm layer was mixed with sterile water at a ratio of 1:(1.5-2.5), and then homogenized using a homogenizer at a speed of 3000-6000 rpm / min for 15-30 min to obtain collagen fiber slurry.

[0032] Furthermore, animal viscera include pig small intestine or bovine or porcine pericardium;

[0033] When the animal viscera are pig small intestines, the pig small intestines are processed to obtain animal-derived biofilms, including:

[0034] Cleanse the small intestine with purified water 3-5 times;

[0035] Cut the cleaned pig small intestine open until it can be flattened, scrape off the mucosal layer, muscular layer and serous layer, and retain the basement membrane, which is the required animal-derived biomembrane;

[0036] When the animal viscera are bovine or porcine pericardial membranes, the bovine or porcine pericardial membranes are processed to obtain animal-derived biofilms, including:

[0037] Wash the bovine or porcine pericardium with purified water 3-5 times;

[0038] The cleaned bovine or porcine pericardium is scraped off, leaving only the corresponding pericardial tissue, which is the desired animal-derived biofilm.

[0039] Further, the preparation of carboxymethyl chitosan slurry includes:

[0040] Take 50g of carboxymethyl chitosan, add 200ml of sterile water, and stir with a mixer at 800-1500rpm / min for 3-5 hours to obtain 200ml of carboxymethyl chitosan slurry;

[0041] Preparation of carboxymethyl chitosan-collagen fiber mixed slurry includes:

[0042] Take 50ml of carboxymethyl chitosan slurry, add 50ml of collagen fiber slurry, and stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of carboxymethyl chitosan-collagen fiber mixed slurry;

[0043] Preparation of a carboxymethyl chitosan slurry-collagen fiber slurry-hydroxyapatite mixed slurry includes:

[0044] Take 30ml of carboxymethyl chitosan slurry, add 25g of hydroxyapatite and 50ml of collagen fiber slurry, and stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of mixed slurry of carboxymethyl chitosan slurry, collagen fiber slurry and hydroxyapatite.

[0045] Furthermore, the connections between the three layers of the dental prosthesis framework are reinforced, including:

[0046] The printed three-layer dental prosthetic framework is immersed in a 5%-10% genipin solution at a ratio of 1:5 for 1-3 hours. After immersion, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time.

[0047] The dental prosthetic framework, which has already been soaked in genipin solution and sterile water, is then soaked in a 10%-15% calcium chloride solution at a ratio of 1:5 for 1-3 hours. After soaking, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time, finally resulting in a reinforced dental prosthetic framework.

[0048] Further processing of the reinforced dental prosthesis includes freeze-drying, packaging, and sterilization.

[0049] Furthermore, the freeze-drying process includes:

[0050] The reinforced dental prosthesis was placed in a freeze dryer and pre-cooled at -70°C for 8 hours.

[0051] Start vacuuming and perform gradient freeze drying for 24-48 hours. After 24-48 hours, turn off the freeze dryer and remove the dental prosthesis.

[0052] Furthermore, packaging and sterilization processes include:

[0053] The freeze-dried dental prosthetic framework was inner-packaged and sterilized by irradiation with a dose of 25-40 KGY to obtain a sterile three-layer dental prosthetic framework, namely a multifunctional bionic dental prosthetic framework.

[0054] Compared with existing technologies, the principles and advantages of this solution are as follows:

[0055] 1. The multifunctional bionic dental prosthesis framework features convex and concave sections, mimicking the natural bonding structure of periodontal connective tissue and alveolar bone. This ensures that fibroblasts are limited from encroaching on osteoblast space while maximizing the preservation of natural growth space for osteoblasts, resulting in a tighter and more stable bond between periodontal tissue and alveolar bone. Furthermore, the multifunctional bionic dental prosthesis framework comprises a three-layer structure: a porous layer that contacts soft tissue and effectively repairs it; a dense layer that effectively blocks fibroblasts; and a bone repair layer that further facilitates alveolar bone repair.

[0056] 2. The loose layer, dense layer, and bone repair layer all contain carboxymethyl chitosan, which promotes wound healing, that is, it facilitates fibrous proliferation and new blood vessel formation. Adding hydroxyapatite to the bone repair layer helps guide bone tissue regeneration.

[0057] 3. Biomembranes are derived from animals, resulting in lower costs. Furthermore, they do not involve complex collagen extraction processes; instead, they extract collagen fibers. Compared to pure collagen oral repair scaffolds, collagen fibers exhibit significantly improved mechanical properties, making them easier to use during surgery.

[0058] 4. Using 3D printing and gradient function settings, a bionic oral restoration framework with multiple functions such as guiding periodontal soft tissue regeneration, guiding bone tissue regeneration, antibacterial and wound healing promotion can be obtained, which is convenient and quick. Attached Figure Description

[0059] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the services required in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0060] Figure 1 This is a perspective view of a multifunctional bionic dental prosthesis frame according to the present invention;

[0061] Figure 2 This is a schematic diagram of the separation of the three-layer structure in the multifunctional bionic oral prosthetic scaffold of the present invention;

[0062] Figure 3 This is a flowchart illustrating the principle of a multifunctional bionic dental prosthesis fabrication method according to the present invention.

[0063] Figure 4 This is a flowchart illustrating the principle of preparing collagen fiber slurry in the fabrication method of a multifunctional biomimetic oral restoration scaffold according to the present invention.

[0064] Figure label:

[0065] 1-Porous layer; 2-Dense layer; 3-Bone repair layer. Detailed Implementation

[0066] The present invention will be further described below with reference to specific embodiments:

[0067] like Figure 1 and Figure 2 As shown in the figure, the multifunctional bionic oral prosthetic scaffold described in this embodiment includes a loose layer 1 that contacts soft tissue, a dense layer 2 that provides physical shielding and antibacterial function, and a bone repair layer 3 that guides bone tissue regeneration. All three have the same shape, are wavy, and have convex and concave parts. The dense layer 2 is located between the loose layer 1 and the bone repair layer 3 and is closely attached to the loose layer 1 and the bone repair layer 3.

[0068] Specifically, in this embodiment, the loose layer 1 is made of a mixture of collagen fibers and carboxymethyl chitosan; the dense layer 2 is made of carboxymethyl chitosan; and the bone repair layer 3 is made of a mixture of carboxymethyl chitosan, hydroxyapatite, and collagen fibers.

[0069] like Figure 3 and Figure 4As shown, the fabrication method of the multifunctional bionic dental prosthesis framework described in this embodiment includes the following steps:

[0070] S1. Preparation of collagen fiber slurry;

[0071] This step specifically includes:

[0072] S1-1. Cleaning: Clean the pig small intestine with purified water 3-5 times;

[0073] S1-2, Scraping: Cut the cleaned pig small intestine open until it can be flattened, scrape off the mucosal layer, muscle layer and serous layer, and retain the basement membrane to obtain the animal-derived biofilm;

[0074] S1-3. Cutting: Cut the animal-derived biofilm into strips of about 10cm using sterile scissors;

[0075] S1-4. Disinfection: Prepare a 0.1-0.5% benzalkonium chloride solution. Take 1 volume of a long strip of animal-derived biofilm and add it to 10 times the volume of benzalkonium chloride solution for disinfection. Soak for 10-30 minutes. After completion, take it out and soak and wash it with sterile water 3-5 times, 10-30 minutes each time.

[0076] S1-5. Virus inactivation: Prepare a NaOH solution with a concentration of 1-4 mol / L. Take 1 volume of sterilized animal biofilm and add it to 10 times the volume of NaOH solution for virus inactivation. Soak for 1-4 hours. After completion, take it out and soak and wash it with sterile water 3-5 times, each time for 10-30 minutes.

[0077] S1-6. Degreasing: Prepare a 0.5-2% sodium deoxycholate solution. Take 1 volume of the animal-derived biofilm that has been inactivated by the virus and add it to 10 times the volume of sodium deoxycholate solution for degreasing. Soak for 24-48 hours. After completion, take it out and soak and wash it with sterile water 3-5 times, 30 minutes each time. Repeat the degreasing step twice.

[0078] S1-7. Decellularization: Prepare a 0.5-1.5% X-100 Triton solution. Take 1 volume of defatted animal biomembrane and add it to 10 volumes of X-100 Triton solution for decellularization. Soak for 24-48 hours. After completion, remove the biomembrane and rinse it with sterile water 3-5 times for 30 minutes each time. Repeat the decellularization step twice to obtain a deantigenated animal biomembrane layer.

[0079] S1-8. Mix the deantigenated animal-derived biofilm layer with sterile water at a ratio of 1:(1.5-2.5), and then homogenize it using a homogenizer at a speed of 3000-6000 rpm / min for 15-30 min to obtain collagen fiber slurry.

[0080] S2. Preparation of carboxymethyl chitosan slurry, the specific process is as follows:

[0081] Take 50g of carboxymethyl chitosan, add 200ml of sterile water, and stir with a mixer at 800-1500rpm / min for 3-5 hours to obtain 200ml of carboxymethyl chitosan slurry.

[0082] S3. Mix carboxymethyl chitosan slurry and collagen fiber slurry to prepare carboxymethyl chitosan-collagen fiber mixed slurry. The specific process is as follows:

[0083] Take 50ml of carboxymethyl chitosan slurry and add 50ml of collagen fiber slurry. Stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of carboxymethyl chitosan-collagen fiber mixed slurry.

[0084] S4. Mix carboxymethyl chitosan slurry, collagen fiber slurry, and hydroxyapatite to prepare a carboxymethyl chitosan slurry-collagen fiber slurry-hydroxyapatite mixed slurry. The specific process is as follows:

[0085] Take 30ml of carboxymethyl chitosan slurry, add 25g of hydroxyapatite and 50ml of collagen fiber slurry, and stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of mixed slurry of carboxymethyl chitosan slurry, collagen fiber slurry and hydroxyapatite.

[0086] S5. Carboxymethyl chitosan slurry, carboxymethyl chitosan-collagen fiber mixed slurry, and carboxymethyl chitosan-collagen fiber-hydroxyapatite mixed slurry are respectively installed in the corresponding nozzles of the 3D printer for printing, thereby obtaining a three-layer oral restoration framework.

[0087] The specific process of this step includes:

[0088] Carboxymethyl chitosan slurry, carboxymethyl chitosan-collagen fiber mixed slurry, and carboxymethyl chitosan-collagen fiber-hydroxyapatite mixed slurry are respectively transferred into the printing cylinder and installed in nozzles 1, 2, and 3 of the 3D printer; the printing parameters are set as follows: the length and width of the print are approximately 20*15mm, the thickness is 0.6-1.8mm, the number of print layers is 3-6, and the layer height is approximately 0.2-0.3mm.

[0089] Printing loose layer 1: No. 2 nozzle first prints 2 loose sub-layers with a spacing of 0.2-0.3mm. The first loose sub-layer is horizontal and the second loose sub-layer is vertical. The total height of the loose layer is 0.2-0.6mm.

[0090] Printing dense layer 2: On the basis of loose layer 1, print head 1 continues to print 2 dense sub-layers. The first dense sub-layer is horizontal and the second dense sub-layer is vertical. The total height of dense layer 2 is 0.2-0.6mm.

[0091] Printing bone repair layer 3: On top of the dense layer 2, nozzle 3 continues to print 2 more bone repair sub-layers. The first bone repair sub-layer is horizontal and the second bone repair sub-layer is vertical. The total height of the dense layer 2 is 0.2-0.6mm.

[0092] (The sub-layers included in each of the loose layer 1, dense layer 2, and bone repair layer 3 are modified according to the actual situation, and there may also be only one sub-layer.)

[0093] S6. Reinforce the connection between the three layers of the dental prosthesis framework;

[0094] This step specifically includes:

[0095] The printed three-layer dental prosthetic framework is immersed in a 5%-10% genipin solution at a ratio of 1:5 for 1-3 hours. After immersion, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time.

[0096] The dental prosthetic framework, which has already been soaked in genipin solution and sterile water, is then soaked in a 10%-15% calcium chloride solution at a ratio of 1:5 for 1-3 hours. After soaking, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time, finally resulting in a reinforced dental prosthetic framework.

[0097] S7. The reinforced dental prosthesis framework is freeze-dried, specifically including:

[0098] The reinforced dental prosthesis was placed in a freeze dryer and pre-cooled at -70°C for 8 hours.

[0099] Start vacuuming and perform gradient freeze drying for 24-48 hours. After 24-48 hours, turn off the freeze dryer and remove the dental prosthesis.

[0100] S8. The freeze-dried dental prosthetic framework is inner-packaged and sterilized by irradiation with a dose of 25-40KGY to obtain a sterile three-layer dental prosthetic framework, namely a multifunctional bionic dental prosthetic framework.

[0101] In this embodiment,

[0102] The multifunctional biomimetic oral restoration framework has convex and concave parts, which simulate the natural combination structure of periodontal connective tissue and alveolar bone. This ensures that fibroblasts are restricted from encroaching on osteoblast space while maximizing the preservation of the natural growth space of osteoblasts, making the periodontal tissue and alveolar bone more tightly and stably integrated.

[0103] In addition, the multifunctional bionic oral restoration framework has a three-layer structure. The loose layer 1 is in contact with soft tissue and can effectively repair soft tissue; the dense layer 2 effectively blocks fibroblasts; and the bone repair layer 3 is more conducive to the repair of alveolar bone.

[0104] Furthermore, biomembranes are derived from animals, resulting in lower costs. Moreover, they do not involve complex collagen extraction processes; instead, they extract collagen fibers. Compared to pure collagen oral repair scaffolds, collagen fibers have significantly improved mechanical properties, making them easier to use during surgery.

[0105] Finally, by using 3D printing and setting gradient functions, a bionic oral restoration framework with multiple functions such as guiding periodontal soft tissue regeneration, guiding bone tissue regeneration, and antibacterial and wound healing promotion can be obtained, which is convenient and quick.

[0106] The above-described embodiments are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Therefore, any changes made in accordance with the shape and principle of the present invention should be covered within the protection scope of the present invention.

Claims

1. A method for fabricating a multifunctional bionic oral prosthetic framework, the multifunctional bionic oral prosthetic framework comprising a loose layer in contact with soft tissue, a dense layer providing physical shielding and antibacterial functions, and a bone repair layer guiding bone tissue regeneration, all three having the same shape, a wavy structure, and having convex and concave portions; the dense layer being disposed between the loose layer and the bone repair layer, and being tightly fitted to both the loose layer and the bone repair layer; the loose layer being made of a mixture of collagen fibers and carboxymethyl chitosan. The dense layer is made of carboxymethyl chitosan material; The bone repair layer is made of a mixture of carboxymethyl chitosan, hydroxyapatite, and collagen fibers, characterized in that the manufacturing method includes: Preparation of collagen fiber slurry; Preparation of carboxymethyl chitosan slurry; Carboxymethyl chitosan slurry and collagen fiber slurry are mixed to prepare carboxymethyl chitosan-collagen fiber mixed slurry; Carboxymethyl chitosan slurry, collagen fiber slurry, and hydroxyapatite are mixed to prepare a carboxymethyl chitosan slurry-collagen fiber slurry-hydroxyapatite mixed slurry; Carboxymethyl chitosan slurry, carboxymethyl chitosan-collagen fiber mixed slurry, and carboxymethyl chitosan-collagen fiber-hydroxyapatite mixed slurry were respectively installed in the corresponding nozzles of the 3D printer for printing, thereby obtaining a three-layer oral restoration framework. Strengthen the connections between the three layers of the dental prosthesis framework; The reinforced dental prosthetic framework is then processed to obtain a multifunctional bionic dental prosthetic framework.

2. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 1, characterized in that, Preparation of collagen fiber slurry includes: Animal viscera are obtained and processed to obtain animal-derived biofilms; Animal-derived biofilms are cut into strips of a predetermined length using sterile scissors; Prepare a 0.1-0.5% benzalkonium chloride solution. Take 1 volume of a long strip of animal-derived biofilm and add it to 10 times the volume of benzalkonium chloride solution for disinfection. Soak for 10-30 minutes. After that, take it out and soak and wash it with sterile water 3-5 times, 10-30 minutes each time. Prepare a NaOH solution with a concentration of 1~4 mol / L. Take 1 volume of sterilized animal-derived biofilm and add it to 10 times the volume of NaOH solution for virus inactivation. Soak for 1~4 hours. After completion, take it out and soak and wash it with sterile water 3~5 times, 10~30 minutes each time. Prepare a 0.5-2% sodium deoxycholate solution. Take 1 volume of animal-derived biofilm that has been inactivated by virus and add it to 10 times the volume of sodium deoxycholate solution for degreasing. Soak for 24-48 hours. After completion, take it out and soak and wash it with sterile water 3-5 times, 30 minutes each time. Repeat the degreasing step twice. Prepare an X-100 Triton solution with a concentration of 0.5-1.5%. Take 1 volume of defatted animal biomembrane and add it to 10 volumes of X-100 Triton solution for decellularization. Soak for 24-48 hours. After completion, remove the biomembrane and soak and wash it with sterile water 3-5 times, 30 minutes each time. Repeat the decellularization step twice to obtain an antigen-free animal biomembrane layer. The deantigenated animal-derived biofilm layer was mixed with sterile water at a ratio of 1:(1.5-2.5), and then homogenized using a homogenizer at a speed of 3000-6000 rpm / min for 15-30 min to obtain collagen fiber slurry.

3. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 2, characterized in that, Animal offal includes pig small intestine or bovine or porcine pericardium; When the animal viscera are pig small intestines, the pig small intestines are processed to obtain animal-derived biofilms, including: Cleanse the small intestine with purified water 3-5 times; Cut the cleaned pig small intestine open until it can be flattened, scrape off the mucosal layer, muscular layer and serous layer, and retain the basement membrane, which is the required animal-derived biomembrane; When the animal viscera are bovine or porcine pericardial membranes, the bovine or porcine pericardial membranes are processed to obtain animal-derived biofilms, including: Wash the bovine or porcine pericardium with purified water 3-5 times; The cleaned bovine or porcine pericardium is scraped off, leaving only the corresponding pericardial tissue, which is the desired animal-derived biofilm.

4. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 1, characterized in that, Preparation of carboxymethyl chitosan slurry includes: Take 50g of carboxymethyl chitosan, add 200ml of sterile water, and stir with a mixer at 800-1500rpm / min for 3-5 hours to obtain 200ml of carboxymethyl chitosan slurry; Preparation of carboxymethyl chitosan-collagen fiber mixed slurry includes: Take 50ml of carboxymethyl chitosan slurry, add 50ml of collagen fiber slurry, and stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of carboxymethyl chitosan-collagen fiber mixed slurry; Preparation of a carboxymethyl chitosan slurry-collagen fiber slurry-hydroxyapatite mixed slurry includes: Take 30ml of carboxymethyl chitosan slurry, add 25g of hydroxyapatite and 50ml of collagen fiber slurry, and stir with a mixer at 800-1000rpm / min for 1-2 hours to obtain 100ml of mixed slurry of carboxymethyl chitosan slurry, collagen fiber slurry and hydroxyapatite.

5. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 1, characterized in that, Strengthening the connections between the three layers of the dental prosthesis framework, including: The printed three-layer dental prosthetic framework is immersed in a 5%-10% genipin solution at a ratio of 1:5 for 1-3 hours. After immersion, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time. The dental prosthetic framework, which has already been soaked in genipin solution and sterile water, is then soaked in a 10%-15% calcium chloride solution at a ratio of 1:5 for 1-3 hours. After soaking, it is removed and rinsed with sterile water 3-5 times for 10-30 minutes each time, finally resulting in a reinforced dental prosthetic framework.

6. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 1, characterized in that, The reinforced dental prosthesis undergoes post-processing, including freeze-drying, packaging, and sterilization.

7. The method for fabricating a multifunctional bionic dental prosthesis according to claim 6, characterized in that, Freeze-drying process, including: The reinforced dental prosthesis was placed in a freeze dryer and pre-cooled at -70°C for 8 hours. Start vacuuming and perform gradient freeze drying for 24-48 hours. After 24-48 hours, turn off the freeze dryer and remove the dental prosthesis.

8. The method for fabricating a multifunctional bionic dental prosthesis framework according to claim 6, characterized in that, Packaging and sterilization processes, including: The freeze-dried dental prosthetic framework was inner-packaged and sterilized by irradiation with a dose of 25-40 KGY to obtain a sterile three-layer dental prosthetic framework, namely a multifunctional bionic dental prosthetic framework.

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