一组叶绿体核周聚集运动的标记物、抑制物及其应用

By using VB1, VB6, and Chitin to activate perinuclear chloroplast aggregation, LATB and DPI to inhibit perinuclear chloroplast aggregation, and Avr2-StTHI4 markers to track chloroplast-nuclear material exchange, the research challenges of perinuclear chloroplast aggregation and exchange mechanisms have been solved, providing molecular tools to support research on plant growth, development, and stress resistance.

CN117849357BActive Publication Date: 2026-07-17WUHAN UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN UNIV
Filing Date
2023-12-28
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The molecular mechanisms and functions of perinuclear chloroplast aggregation and dissociation are still unclear, and there is a lack of effective molecular tools for studying chloroplast movement and chloroplast-nuclear material exchange.

Method used

Vitamin B1 (VB1), vitamin B6 (VB6), and chitin were used as markers to activate perinuclear chloroplast aggregation, while LATB and DPI were used as inhibitors to suppress perinuclear chloroplast aggregation. Avr2-StTHI4 was used as a marker to track chloroplast-nuclear material exchange.

Benefits of technology

It provides visualized molecular tools to study the perinuclear aggregation function of chloroplasts and the mechanism of material information exchange, thus promoting our understanding of plant growth, development and stress resistance.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一组叶绿体核周聚集运动的标记物、抑制物及其应用。本发明中维生素B1(VB1)能显著诱导植物叶绿体在细胞核周围形成聚集;另有维生素B6(VB6)、几丁质(Chitin)等可以激活植物活性氧(ROS)积累的物质也可以触发核周叶绿体聚集现象;微丝抑制剂Latrunculin B(LATB)和活性氧抑制剂Diphenyleneiodonium chloride(DPI)均可显著抑制叶绿体核周聚集。在致病疫霉效应蛋白Avr2诱导下,StTHI4通过核周叶绿体聚集结构进入细胞核,融合荧光集团的StTHI4蛋白可以作为叶绿体‑细胞核发生物质信息交换的可视化标记探针。
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